Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,008,480 +A coding (637/654 nt) yedK → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,008,4761.A99.0% 708.3 / ‑1.7 196coding (633/654 nt)yedKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base A (90/104);  total (91/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 223 total aligned reads displayed.

AGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA  >  NC_000913/2008384‑2008563
                                                                                              |                                                                                       
aGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑Gtt                                                                                          <  2:377657/93‑1 (MQ=255)
 gCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑a                                                                                        >  2:1597086/1‑93 (MQ=255)
 gCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGGGGGTAAT‑GTT‑a                                                                                        >  2:2309133/1‑93 (MQ=255)
  cGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aa                                                                                       <  1:1686473/93‑1 (MQ=255)
    gTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aaaa                                                                                     >  1:4243489/1‑93 (MQ=255)
    gTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aaaa                                                                                     >  1:7877913/1‑93 (MQ=255)
    gTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aaaa                                                                                     <  1:4487410/93‑1 (MQ=255)
     tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aaaaa                                                                                    <  1:7367234/93‑2 (MQ=255)
     tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aaaaa                                                                                    <  1:4090806/93‑2 (MQ=255)
     tAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aaaaa                                                                                    <  1:5829730/93‑2 (MQ=255)
       aagaagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑aaaaacc                                                                                  <  1:4140573/93‑4 (MQ=255)
          aagCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAgg                                                                                >  2:5695586/1‑93 (MQ=255)
           agCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAggg                                                                               >  1:975423/1‑93 (MQ=255)
            gCCTCAGAAATCGCGGCCAGCGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGgc                                                                              >  2:7605669/1‑93 (MQ=255)
              cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGgcgc                                                                            <  2:5086405/93‑1 (MQ=255)
              cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGgcgc                                                                            >  1:306027/1‑93 (MQ=255)
              cTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGgcgc                                                                            <  1:6863016/93‑1 (MQ=255)
               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGcgcg                                                                           >  1:3433497/1‑93 (MQ=255)
               tCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGcgcg                                                                           <  2:5993254/93‑1 (MQ=255)
                 aGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGa                                                                         >  2:3798504/1‑93 (MQ=255)
                 aGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGa                                                                         <  1:6008020/93‑1 (MQ=255)
                 aGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGa                                                                         <  1:6510277/93‑1 (MQ=255)
                  gAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTACCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAg                                                                        <  2:5386575/93‑1 (MQ=255)
                   aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGt                                                                       <  1:4769348/93‑1 (MQ=255)
                   aaaTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGt                                                                       <  1:7559997/93‑1 (MQ=255)
                     aTCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTa                                                                     >  1:5703048/1‑93 (MQ=255)
                       cgcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAAt                                                                   <  2:4254540/93‑1 (MQ=255)
                       cgcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAAt                                                                   <  1:3247640/93‑1 (MQ=255)
                        gcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAAtt                                                                  >  1:1093314/1‑93 (MQ=255)
                        gcgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAAtt                                                                  >  2:2064289/1‑93 (MQ=255)
                         cgGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTc                                                                 <  1:6156893/93‑1 (MQ=255)
                          ggCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCa                                                                >  1:7205098/1‑93 (MQ=255)
                          ggCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCa                                                                >  2:6831698/1‑93 (MQ=255)
                           gCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCaa                                                               >  2:1394311/1‑93 (MQ=255)
                           gCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCaa                                                               <  2:1326220/93‑1 (MQ=255)
                            ccAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAAc                                                              <  1:3958686/93‑1 (MQ=255)
                             cAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAAcc                                                             <  2:2177440/93‑1 (MQ=255)
                             cAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAAcc                                                             >  2:7680228/1‑93 (MQ=255)
                              aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCt                                                            >  1:2435795/1‑93 (MQ=255)
                              aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCt                                                            <  1:59189/93‑1 (MQ=255)
                              aGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGCATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAAccg                                                            >  2:4546254/1‑92 (MQ=255)
                               gTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTg                                                           >  2:1640723/1‑93 (MQ=255)
                               gTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTg                                                           >  2:7369032/1‑93 (MQ=255)
                                  gCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGttt                                                        >  2:4751464/1‑93 (MQ=255)
                                  gCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGttt                                                        >  1:1068340/1‑93 (MQ=255)
                                   cTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTg                                                       >  2:5919993/1‑93 (MQ=255)
                                    tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                      >  2:6948094/1‑93 (MQ=255)
                                    tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                      <  1:4986424/93‑1 (MQ=255)
                                    tgtgTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGa                                                      >  2:2427616/1‑93 (MQ=255)
                                     gtgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGAt                                                     <  2:1527513/93‑1 (MQ=255)
                                      tgtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATa                                                    <  2:1470354/93‑1 (MQ=255)
                                       gtTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATaa                                                   <  1:6304972/93‑1 (MQ=255)
                                          tcAGGAACCCAGGTCTCCTGGCCGCCGGTAACGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGcc                                                <  1:1208818/92‑1 (MQ=255)
                                          ccAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGcc                                                <  1:2932070/93‑1 (MQ=255)
                                           cAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCt                                               <  1:754122/93‑1 (MQ=255)
                                           cAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGAGCGGAGTTAATTCAACCTGTTTGATAAGCCt                                               <  2:3753787/93‑1 (MQ=255)
                                             gCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTgg                                             <  2:1226513/93‑1 (MQ=255)
                                             gCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTgg                                             >  2:541979/1‑93 (MQ=255)
                                              gaaaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGga                                            <  1:5770771/92‑1 (MQ=255)
                                               aaaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgag                                           >  2:7201753/1‑93 (MQ=255)
                                                aaCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGgaga                                          >  1:7807090/1‑93 (MQ=255)
                                                 aCCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGat                                         >  1:6676510/1‑93 (MQ=255)
                                                    aGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGattat                                      <  1:4382348/93‑1 (MQ=255)
                                                       tctcCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGa                                   <  2:6970607/93‑1 (MQ=255)
                                                        ctcCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAt                                  <  1:4345477/93‑1 (MQ=255)
                                                        ctcCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAt                                  <  1:5873814/93‑1 (MQ=255)
                                                         tcCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATc                                 <  2:4846739/93‑1 (MQ=255)
                                                          ccTGGCACGCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATca                                <  2:3088733/93‑1 (MQ=255)
                                                             ggCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaaca                             >  2:7504990/1‑93 (MQ=255)
                                                              gCACCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATcaacaa                            >  1:6265383/1‑93 (MQ=255)
                                                                ccccGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCACCCTGTTTGATAAGCCTGGAGATTATTGATCAACAATa                          <  2:649071/92‑1 (MQ=255)
                                                                aCCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATa                          <  1:3033910/93‑1 (MQ=255)
                                                                aCCCGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATAAACAATa                          <  1:944470/93‑1 (MQ=255)
                                                                   cGGTATCGCGCGCGGTGGGTAATGGTA‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTg                       >  2:3734380/1‑93 (MQ=255)
                                                                   cGGTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTg                       >  1:5114315/1‑93 (MQ=255)
                                                                    ggTATCGCGCGCGGTGTGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGc                      <  2:1393184/93‑1 (MQ=255)
                                                                    ggTATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGc                      <  1:1897584/93‑1 (MQ=255)
                                                                      tATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGt                    <  1:673993/93‑1 (MQ=255)
                                                                      tATCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGt                    <  2:4684258/93‑1 (MQ=255)
                                                                       aTCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTc                   >  2:5333230/1‑93 (MQ=255)
                                                                       aTCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTc                   <  1:2131675/93‑1 (MQ=255)
                                                                        tCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCa                  >  2:5158428/1‑93 (MQ=255)
                                                                        tCGCGCGCGGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCa                  <  1:5574642/93‑1 (MQ=255)
                                                                         cgcgcgcgGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCAt                 <  2:3482365/93‑1 (MQ=255)
                                                                              gcgGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaa            <  1:7647718/93‑1 (MQ=255)
                                                                               cgGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTGAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa           <  1:6331426/93‑1 (MQ=255)
                                                                               cgGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa           >  2:3909558/1‑93 (MQ=255)
                                                                               cgGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa           <  1:6051861/93‑1 (MQ=255)
                                                                               cgGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa           <  2:1890617/93‑1 (MQ=255)
                                                                               cgGTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa           <  1:5535248/93‑1 (MQ=255)
                                                                               cgGTGGATAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGaaa           <  1:5261252/93‑1 (MQ=255)
                                                                                 gTGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtc         <  2:6226784/93‑1 (MQ=255)
                                                                                  tGGGTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtct        >  1:3167439/1‑93 (MQ=255)
                                                                                   gggTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       <  1:3808344/93‑1 (MQ=255)
                                                                                   gggTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAtctc       <  1:4519293/93‑1 (MQ=255)
                                                                                    ggTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATctct      >  1:1254368/1‑93 (MQ=255)
                                                                                     gTAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAACAAATctctt     >  1:2627946/1‑92 (MQ=255)
                                                                                      tAAT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAt    >  1:1008697/1‑93 (MQ=255)
                                                                                       aaT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAtt   <  1:1802262/93‑1 (MQ=255)
                                                                                        aT‑GTTAAAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTa  >  1:4327118/1‑93 (MQ=255)
                                                                                              |                                                                                       
AGCGGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAAT‑GTT‑AAAACCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA  >  NC_000913/2008384‑2008563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  >  NC_000913/2008387‑2008573
                                                                                               |                                                                                              
GGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A                                                                                                >  SRR4302543.7952066/1‑94 (MQ=60)
GGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A                                                                                                >  SRR4302543.4281996/1‑94 (MQ=60)
GGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGGTCCAGCAAACCAGTTCTCCTGGCCCCCGGGATCGCGCGCGGTGGGTAATGTT‑AAA‑A                                                                                                >  SRR4302543.4281997/1‑94 (MQ=60)
 GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑A                                                                                              >  SRR4302543.952537/1‑94 (MQ=60)
 GTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑A                                                                                              <  SRR4302543.4528172/94‑1 (MQ=60)
  TAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑AC                                                                                             <  SRR4302543.4127495/94‑1 (MQ=60)
  TAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑AC                                                                                             <  SRR4302543.1231810/94‑1 (MQ=60)
  TAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑AC                                                                                             <  SRR4302543.7438072/94‑1 (MQ=60)
  TAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑AC                                                                                             <  SRR4302543.5327018/94‑1 (MQ=60)
  TAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑AC                                                                                             <  SRR4302543.5882752/94‑1 (MQ=60)
  TAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑AC                                                                                             <  SRR4302543.5882753/94‑1 (MQ=60)
   AAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑Aacc                                                                                             >  SRR4302543.427922/1‑91 (MQ=60)
    AAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCA                                                                                            <  SRR4302543.4177961/94‑1 (MQ=60)
    AAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCA                                                                                            >  SRR4302543.3118539/1‑94 (MQ=60)
     AGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAG                                                                                           >  SRR4302543.957785/1‑94 (MQ=60)
      GAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGG                                                                                          >  SRR4302543.5600/1‑94 (MQ=60)
      GAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGG                                                                                          <  SRR4302543.3191428/94‑1 (MQ=60)
       AAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGG                                                                                         >  SRR4302543.984019/1‑94 (MQ=60)
         GCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCG                                                                                       <  SRR4302543.5854090/94‑1 (MQ=60)
          CCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGC                                                                                      >  SRR4302543.308791/1‑94 (MQ=60)
           CTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCG                                                                                     >  SRR4302543.3464224/1‑94 (MQ=60)
           CTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCG                                                                                     >  SRR4302543.3450554/1‑94 (MQ=60)
           CTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCG                                                                                     <  SRR4302543.6927683/94‑1 (MQ=60)
           CTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCG                                                                                     >  SRR4302543.4441759/1‑94 (MQ=60)
            TCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGG                                                                                    >  SRR4302543.6505009/1‑94 (MQ=60)
            TCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGG                                                                                    <  SRR4302543.6342137/94‑1 (MQ=60)
              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAG                                                                                  <  SRR4302543.4022451/94‑1 (MQ=60)
              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAG                                                                                  <  SRR4302543.2168241/94‑1 (MQ=60)
              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAG                                                                                  <  SRR4302543.7365840/94‑1 (MQ=60)
              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAG                                                                                  >  SRR4302543.3103992/1‑94 (MQ=60)
              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAG                                                                                  <  SRR4302543.6571601/94‑1 (MQ=60)
              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAG                                                                                  >  SRR4302543.2093894/1‑94 (MQ=60)
              AGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAG                                                                                  <  SRR4302543.6063356/94‑1 (MQ=60)
                AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTT                                                                                <  SRR4302543.7631964/94‑1 (MQ=60)
                AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTT                                                                                <  SRR4302543.4812939/94‑1 (MQ=60)
                AAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTT                                                                                <  SRR4302543.6119201/94‑1 (MQ=60)
                 AATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTA                                                                               >  SRR4302543.5755064/1‑94 (MQ=60)
                    CGCGGCCAGTGGCTGTGTTCCAGCAAACCGGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑ACCCAGGGCGCGGCGTTAATT                                                                            >  SRR4302543.1103147/1‑94 (MQ=60)
                    CGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATT                                                                            >  SRR4302543.1103148/1‑94 (MQ=60)
                    CGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATT                                                                            <  SRR4302543.3276999/94‑1 (MQ=60)
                     GCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTC                                                                           <  SRR4302543.4555757/94‑1 (MQ=60)
                      CGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCA                                                                          >  SRR4302543.7274951/1‑94 (MQ=60)
                      CGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCA                                                                          <  SRR4302543.6215171/94‑1 (MQ=60)
                       GGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAA                                                                         <  SRR4302543.5204786/94‑1 (MQ=60)
                         CCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACC                                                                       <  SRR4302543.3993733/94‑1 (MQ=60)
                          CAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCT                                                                      <  SRR4302543.6581132/94‑1 (MQ=60)
                          CAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCT                                                                      >  SRR4302543.2457768/1‑94 (MQ=60)
                           AGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTG                                                                     <  SRR4302543.59708/94‑1 (MQ=60)
                            GTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGT                                                                    <  SRR4302543.4695869/94‑1 (MQ=60)
                              GGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTT                                                                  >  SRR4302543.2636333/1‑94 (MQ=60)
                              GGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTT                                                                  >  SRR4302543.1077942/1‑94 (MQ=60)
                               GCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTG                                                                 >  SRR4302543.969301/1‑94 (MQ=60)
                                 TGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGAT                                                               >  SRR4302543.6405145/1‑94 (MQ=60)
                                 TGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGAT                                                               <  SRR4302543.5031813/94‑1 (MQ=60)
                                  GTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATA                                                              <  SRR4302543.3940820/94‑1 (MQ=60)
                                    GTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAG                                                            <  SRR4302543.1104804/94‑1 (MQ=60)
                                    GTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAG                                                            <  SRR4302543.6364283/94‑1 (MQ=60)
                                       tcaggaacccaggTCTCCTGGCCGCCGGTAACGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCT                                                         <  SRR4302543.1219693/81‑1 (MQ=60)
                                       CCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCT                                                         <  SRR4302543.2958695/94‑1 (MQ=60)
                                        CAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTG                                                        <  SRR4302543.760795/94‑1 (MQ=60)
                                         AGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGG                                                       <  SRR4302543.4332502/94‑1 (MQ=60)
                                         AGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGG                                                       <  SRR4302543.4676555/94‑1 (MQ=60)
                                          GCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGA                                                      >  SRR4302543.7626313/1‑94 (MQ=60)
                                           GAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAG                                                     <  SRR4302543.5823317/94‑1 (MQ=60)
                                           CAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAG                                                     >  SRR4302543.2755327/1‑94 (MQ=60)
                                            AAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGA                                                    >  SRR4302543.7880682/1‑94 (MQ=60)
                                            AAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGA                                                    >  SRR4302543.2290229/1‑94 (MQ=60)
                                             AACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGAT                                                   <  SRR4302543.4137497/94‑1 (MQ=60)
                                             AACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGAT                                                   >  SRR4302543.6739542/1‑94 (MQ=60)
                                               CCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTA                                                 >  SRR4302543.6971763/1‑94 (MQ=60)
                                               CCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTA                                                 <  SRR4302543.4380262/94‑1 (MQ=60)
                                                CAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTAT                                                >  SRR4302543.1320723/1‑94 (MQ=60)
                                                 AGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATT                                               <  SRR4302543.4422074/94‑1 (MQ=60)
                                                   TTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGA                                             >  SRR4302543.5725747/1‑94 (MQ=60)
                                                    TCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAT                                            <  SRR4302543.7858730/94‑1 (MQ=60)
                                                    TCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAT                                            <  SRR4302543.5796990/94‑1 (MQ=60)
                                                    TCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGAT                                            >  SRR4302543.5832121/1‑94 (MQ=60)
                                                     CTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATC                                           <  SRR4302543.4384881/94‑1 (MQ=60)
                                                     CTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATC                                           <  SRR4302543.5927315/94‑1 (MQ=60)
                                                        CTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAAC                                        <  SRR4302543.5324456/94‑1 (MQ=60)
                                                        CTGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAAC                                        >  SRR4302543.1068096/1‑94 (MQ=60)
                                                         TGGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACA                                       <  SRR4302543.6856074/94‑1 (MQ=60)
                                                          GGCACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAA                                      >  SRR4302543.6324459/1‑94 (MQ=60)
                                                          GGCACCCGGTATCGCGCGCGGGGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAGTTCAACCTGGTTGATCAGCCTGGAGATTATTGACCACCAA                                      >  SRR4302543.6778805/1‑94 (MQ=60)
                                                             ACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAC                                   <  SRR4302543.6195653/94‑1 (MQ=60)
                                                             ACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAC                                   >  SRR4302543.1714412/1‑94 (MQ=60)
                                                             ACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAC                                   <  SRR4302543.3061299/94‑1 (MQ=60)
                                                             ACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATAC                                   >  SRR4302543.5153198/1‑94 (MQ=60)
                                                             ACCCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATAAACAATAC                                   <  SRR4302543.952726/94‑1 (MQ=60)
                                                               CCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTG                                 <  SRR4302543.5937473/94‑1 (MQ=60)
                                                               CCGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTG                                 >  SRR4302543.5160581/1‑94 (MQ=60)
                                                                CGGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGC                                <  SRR4302543.3909325/94‑1 (MQ=60)
                                                                 GGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCG                               >  SRR4302543.135869/1‑94 (MQ=60)
                                                                 GGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCG                               >  SRR4302543.3069919/1‑94 (MQ=60)
                                                                 GGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCG                               <  SRR4302543.1914656/94‑1 (MQ=60)
                                                                 GGTATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCG                               <  SRR4302543.1908822/94‑1 (MQ=60)
                                                                   TATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTC                             >  SRR4302543.6244979/1‑94 (MQ=60)
                                                                   TATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTC                             <  SRR4302543.680195/94‑1 (MQ=60)
                                                                    ATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCA                            >  SRR4302543.602337/1‑94 (MQ=60)
                                                                    ATCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCA                            <  SRR4302543.2150758/94‑1 (MQ=60)
                                                                     TCGCGCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCAT                           <  SRR4302543.5625382/94‑1 (MQ=60)
                                                                         GCGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGA                       <  SRR4302543.6935470/94‑1 (MQ=60)
                                                                          CGCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAA                      >  SRR4302543.3528337/1‑94 (MQ=60)
                                                                           GCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAA                     <  SRR4302543.7720234/94‑1 (MQ=60)
                                                                           GCGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAA                     <  SRR4302543.7720235/94‑1 (MQ=60)
                                                                            CTGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAGTTGAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAT                    <  SRR4302543.6390971/94‑1 (MQ=60)
                                                                            CGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTGAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAT                    <  SRR4302543.6390972/94‑1 (MQ=60)
                                                                            CGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAT                    <  SRR4302543.6109148/94‑1 (MQ=60)
                                                                            CGGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAT                    <  SRR4302543.5585551/94‑1 (MQ=60)
                                                                            CGGTGGGTAATGTT‑AAACA‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAT                    <  SRR4302543.6109147/94‑1 (MQ=60)
                                                                            CGGTGGATAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAAT                    <  SRR4302543.5308715/94‑1 (MQ=60)
                                                                             GGTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATC                   >  SRR4302543.7784065/1‑94 (MQ=60)
                                                                              GTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCT                  <  SRR4302543.3207812/94‑1 (MQ=60)
                                                                              GTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCT                  <  SRR4302543.7943323/94‑1 (MQ=60)
                                                                              GTGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCT                  >  SRR4302543.3195960/1‑94 (MQ=60)
                                                                               TGGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTC                 >  SRR4302543.2996453/1‑94 (MQ=60)
                                                                                GGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCT                <  SRR4302543.4560400/94‑1 (MQ=60)
                                                                                GGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCT                <  SRR4302543.3841922/94‑1 (MQ=60)
                                                                                GGGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCT                >  SRR4302543.1265612/1‑94 (MQ=60)
                                                                                 GGTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAACAAATCTCTT               >  SRR4302543.2651727/1‑94 (MQ=60)
                                                                                  GTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAT              >  SRR4302543.7530749/1‑94 (MQ=60)
                                                                                  GTAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTAT              >  SRR4302543.1017620/1‑94 (MQ=60)
                                                                                   TAATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATT             >  SRR4302543.502326/1‑94 (MQ=60)
                                                                                    AATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA            <  SRR4302543.1818392/94‑1 (MQ=60)
                                                                                    AATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTA            >  SRR4302543.4366396/1‑94 (MQ=60)
                                                                                     ATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAG           >  SRR4302543.1184000/1‑94 (MQ=60)
                                                                                     ATGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAG           >  SRR4302543.6521450/1‑94 (MQ=60)
                                                                                      TGTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGA          >  SRR4302543.2295654/1‑94 (MQ=60)
                                                                                       GTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGAC         <  SRR4302543.2934659/94‑1 (MQ=60)
                                                                                       GTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGAC         >  SRR4302543.7501071/1‑94 (MQ=60)
                                                                                       GTTAAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGAC         <  SRR4302543.1611335/94‑1 (MQ=60)
                                                                                        ttaAAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACA        >  SRR4302543.4339915/4‑94 (MQ=60)
                                                                                           AAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAG     <  SRR4302543.5376166/94‑1 (MQ=60)
                                                                                            AA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGA    <  SRR4302543.751934/94‑1 (MQ=60)
                                                                                               A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  <  SRR4302543.3272348/94‑1 (MQ=60)
                                                                                               A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  <  SRR4302543.2168279/94‑1 (MQ=60)
                                                                                               |                                                                                              
GGTAAAGAAGCCTCAGAAATCGCGGCCAGTGGCTGTGTTCCAGCAAACCAGTTCTCCTGGCACCCGGTATCGCGCGCGGTGGGTAATGTT‑AAA‑A‑CCAGGGCGCGGAGTTAATTCAACCTGTTTGATAAGCCTGGAGATTATTGATCAACAATACTGCGTCATAAGAAATCTCTATTAGACAAAGATT  >  NC_000913/2008387‑2008573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑