Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,069,029	T→C	S169S (AGT→AGC)	yeeP →	putative histone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,069,029	0	T	C	99.0%	299.5 / ‑4.7	99	S169S (AGT→AGC)	yeeP	putative histone
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (56/42); total (57/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 108 total aligned reads displayed.

TCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGAT  >  NC_000913/2068939‑2069118

tCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGccc                                                                                         >  2:1425038/1‑93 (MQ=255)

 ccGGTTGTGGCCGTATCGGCCCGCACGGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATTACAGCTCTTCCCGACCATGCCGCCAGcccc                                                                                        <  1:1644245/93‑1 (MQ=255)

 ccGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGcccc                                                                                        >  2:1964289/1‑93 (MQ=255)

  cGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCcccc                                                                                       <  2:150261/93‑1 (MQ=255)

  cGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCcccc                                                                                       <  1:1374589/93‑1 (MQ=255)

   ggTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCt                                                                                      >  2:2265612/1‑93 (MQ=255)

    gTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtg                                                                                     <  2:4063470/93‑1 (MQ=255)

     ttGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtga                                                                                    <  1:2463617/93‑1 (MQ=255)

     ttGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtga                                                                                    >  2:4588848/1‑93 (MQ=255)

      tgtgGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgat                                                                                   <  1:3893585/93‑1 (MQ=255)

       gtgGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgatg                                                                                  >  2:2621774/1‑93 (MQ=255)

       gtgGCCGGATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCtgatg                                                                                  >  2:4608360/1‑93 (MQ=255)

          gCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGAcc                                                                               <  2:1371840/93‑1 (MQ=255)

          gCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGAcc                                                                               >  1:4159361/1‑93 (MQ=255)

            cGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGGGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCg                                                                             >  1:2510881/1‑93 (MQ=255)

             gTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGa                                                                            >  1:3415795/1‑93 (MQ=255)

             gTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGa                                                                            <  2:2644422/93‑1 (MQ=255)

              tATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAc                                                                           <  1:2040018/93‑1 (MQ=255)

              tATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAc                                                                           <  1:4608125/93‑1 (MQ=255)

              tATCGGCCCGCACCGGATGGGAACTGGGTACGCTGGGCAGGGCGCTCATGATCGCGCTTCCCGACCCTGCCGGCCGCCCACTGATGACCCGAc                                                                           >  2:1629343/1‑93 (MQ=255)

               aTCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACt                                                                          >  2:2636558/1‑93 (MQ=255)

                tCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTg                                                                         >  1:3986389/1‑93 (MQ=255)

                tCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTg                                                                         >  1:4213378/1‑93 (MQ=255)

                tCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTg                                                                         >  2:260013/1‑93 (MQ=255)

                  ggCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCa                                                                       <  2:1338915/93‑1 (MQ=255)

                   gCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAg                                                                      <  2:5058/93‑1 (MQ=255)

                   gCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAg                                                                      >  1:497240/1‑93 (MQ=255)

                     ccGCGCCGGCTGGGAACTGCATACGGTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGa                                                                    <  1:3655138/93‑1 (MQ=255)

                     ccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGa                                                                    <  2:3052095/93‑1 (MQ=255)

                     ccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGa                                                                    <  1:1253606/93‑1 (MQ=255)

                     ccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGa                                                                    <  2:3836449/93‑1 (MQ=255)

                     ccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGa                                                                    <  2:2013906/93‑1 (MQ=255)

                     ccGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTACCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGa                                                                    >  1:2442321/1‑93 (MQ=255)

                     ccGCACCGGCTGGGAACTGGATAAGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGAATGCAGGa                                                                    >  2:4261719/1‑93 (MQ=255)

                       gCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGAAAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACg                                                                  >  2:2073875/1‑93 (MQ=255)

                        cACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGa                                                                 >  2:3029684/1‑93 (MQ=255)

                           cGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCt                                                              >  1:1683169/1‑93 (MQ=255)

                           cGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCt                                                              >  2:598675/1‑93 (MQ=255)

                            ggCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTg                                                             <  1:2751153/93‑1 (MQ=255)

                            ggCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTg                                                             <  2:1904584/93‑1 (MQ=255)

                            ggCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTg                                                             >  2:3433140/1‑93 (MQ=255)

                              cTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgcg                                                           >  1:738995/1‑93 (MQ=255)

                               tGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgcgc                                                          <  1:3883182/93‑1 (MQ=255)

                               tGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTgcgc                                                          >  2:311337/1‑93 (MQ=255)

                                gggAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCa                                                         >  1:1362929/1‑93 (MQ=255)

                                 ggAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCAc                                                        >  1:1213723/1‑93 (MQ=255)

                                      tGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAAt                                                   >  2:975395/1‑93 (MQ=255)

                                       ggATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATc                                                  >  1:2931854/1‑93 (MQ=255)

                                       ggATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATc                                                  <  2:348212/93‑1 (MQ=255)

                                         aTACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTg                                                >  2:4474465/1‑93 (MQ=255)

                                            cGCTGTTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTcc                                             <  2:1824218/93‑1 (MQ=255)

                                                ggTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGAGCACGGAATCTGTCCGGtc                                         >  2:787586/1‑93 (MQ=255)

                                                 gTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtct                                        <  2:1719381/93‑1 (MQ=255)

                                                 gTCAGTCCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGtct                                        <  2:706586/93‑1 (MQ=255)

                                                   cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                      <  1:672969/93‑1 (MQ=255)

                                                   cAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCa                                      <  2:384474/93‑1 (MQ=255)

                                                    aGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAg                                     <  1:3311251/93‑1 (MQ=255)

                                                         gcTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCg                                <  2:3028512/93‑1 (MQ=255)

                                                           tCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTg                              >  1:250312/1‑93 (MQ=255)

                                                           tCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTg                              >  2:3825468/1‑93 (MQ=255)

                                                           tCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTg                              >  2:449615/1‑93 (MQ=255)

                                                             aTGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGaa                            <  1:1080328/93‑1 (MQ=255)

                                                              tGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAAc                           >  1:3727796/1‑93 (MQ=255)

                                                               gACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACa                          <  2:1488722/93‑1 (MQ=255)

                                                               gACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACa                          <  2:747526/93‑1 (MQ=255)

                                                                aCAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAg                         >  2:2435962/1‑93 (MQ=255)

                                                                aCAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAg                         >  2:616327/1‑93 (MQ=255)

                                                                aCAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAg                         <  1:3430204/93‑1 (MQ=255)

                                                                 cAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGt                        >  2:1245541/1‑93 (MQ=255)

                                                                  aGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGtt                       >  1:705585/1‑93 (MQ=255)

                                                                  aGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGtt                       <  1:498738/93‑1 (MQ=255)

                                                                  aGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGtt                       >  1:29555/1‑93 (MQ=255)

                                                                  aGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGtt                       <  1:1839881/93‑1 (MQ=255)

                                                                   gcgcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGttt                      >  2:2733236/1‑93 (MQ=255)

                                                                   gcgcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGttt                      <  1:3874320/93‑1 (MQ=255)

                                                                   gcgcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGttt                      >  2:3708798/1‑93 (MQ=255)

                                                                     gcTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGCCCCGTGAACAGTTTAc                    >  2:2550112/1‑93 (MQ=255)

                                                                      cTTCCCGACCATGCCTCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGTGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAcc                   <  1:39301/93‑1 (MQ=255)

                                                                      cTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTAcc                   >  2:537934/1‑93 (MQ=255)

                                                                       ttCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                  >  1:3519242/1‑93 (MQ=255)

                                                                       ttCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                  >  1:1059921/1‑93 (MQ=255)

                                                                       ttCCCGACCATGCCGCCAGACCCCTGATGACCCGACTGCAGGACGAGCGGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcg                  <  2:785237/93‑1 (MQ=255)

                                                                        tCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg                 <  2:723986/93‑1 (MQ=255)

                                                                        tCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcgg                 >  2:4168290/1‑93 (MQ=255)

                                                                         cccGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggt                <  1:1656823/93‑1 (MQ=255)

                                                                         cccGACCAGGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggt                <  1:1744454/93‑1 (MQ=255)

                                                                           cGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgc              >  2:3999889/1‑93 (MQ=255)

                                                                            gACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcg             >  1:3897890/1‑93 (MQ=255)

                                                                             aCCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcgg            >  1:3852414/1‑93 (MQ=255)

                                                                             aCCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcgg            <  1:704138/93‑1 (MQ=255)

                                                                              ccATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggt           >  2:1605393/1‑93 (MQ=255)

                                                                              ccATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggt           <  1:3790283/93‑1 (MQ=255)

                                                                              ccATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggt           >  1:3027267/1‑93 (MQ=255)

                                                                              ccATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACcggtgcggt           <  1:2878648/93‑1 (MQ=255)

                                                                                aTGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTgg         >  1:1029183/1‑93 (MQ=255)

                                                                                  gccgccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGAc       <  1:795862/93‑1 (MQ=255)

                                                                                     gccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCgg    >  2:830814/1‑93 (MQ=255)

                                                                                      ccAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGa   >  1:4534462/1‑93 (MQ=255)

                                                                                       cAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGat  >  2:2333427/1‑93 (MQ=255)

                                                                                       cAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGGGGACCggct  >  2:2459774/1‑91 (MQ=255)

TCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGAT  >  NC_000913/2068939‑2069118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTT  >  NC_000913/2068936‑2069122

ACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGC                                                                                               <  SRR4302535.4364616/94‑1 (MQ=60)

    CCGGTTGTGGCCGTATCGGCCCGCACGGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATTACAGCTCTTCCCGACCATGCCGCCAGCCCCC                                                                                           <  SRR4302535.1656130/94‑1 (MQ=60)

     CGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCT                                                                                          <  SRR4302535.1384410/94‑1 (MQ=60)

        TTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGAT                                                                                       <  SRR4302535.2481381/94‑1 (MQ=60)

         TGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATG                                                                                      <  SRR4302535.3920273/94‑1 (MQ=60)

            GGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACC                                                                                   >  SRR4302535.4187827/1‑94 (MQ=60)

              CCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGGGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCG                                                                                 >  SRR4302535.2528980/1‑94 (MQ=60)

               CGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGA                                                                                >  SRR4302535.3439557/1‑94 (MQ=60)

                 TATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACT                                                                              <  SRR4302535.2054711/94‑1 (MQ=60)

                 TATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACT                                                                              <  SRR4302535.4639440/94‑1 (MQ=60)

                  ATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTG                                                                             >  SRR4302535.4242213/1‑94 (MQ=60)

                  ATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTG                                                                             >  SRR4302535.4013633/1‑94 (MQ=60)

                     GGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAG                                                                          >  SRR4302535.500729/1‑94 (MQ=60)

                       CCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTACCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGA                                                                        >  SRR4302535.2459915/1‑94 (MQ=60)

                       CCCGCACCGGCTGGGAACTGGATACCCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATTACCCCACTGCcggc                                                                        >  SRR4302535.2132508/1‑90 (MQ=60)

                        CCGCGCCGGCTGGGAACTGCATACGGTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAC                                                                       <  SRR4302535.3680439/94‑1 (MQ=60)

                        CCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGAC                                                                       <  SRR4302535.1262570/94‑1 (MQ=60)

                          GCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGA                                                                     >  SRR4302535.2334343/1‑94 (MQ=60)

                             CCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCT                                                                  >  SRR4302535.1695362/1‑94 (MQ=60)

                               GGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGC                                                                <  SRR4302535.2770765/94‑1 (MQ=60)

                                GCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCG                                                               >  SRR4302535.744192/1‑94 (MQ=60)

                                  TGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCA                                                             <  SRR4302535.3909822/94‑1 (MQ=60)

                                  TGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCA                                                             >  SRR4302535.1372676/1‑94 (MQ=60)

                                   GGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCAC                                                            >  SRR4302535.1222428/1‑94 (MQ=60)

                                         TGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATC                                                      >  SRR4302535.2952496/1‑94 (MQ=60)

                                          GGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGGTGCGCACGcgaact                                                     >  SRR4302535.4689636/1‑88 (MQ=60)

                                                      CAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAG                                         <  SRR4302535.677665/94‑1 (MQ=60)

                                                       AGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGG                                        <  SRR4302535.3334295/94‑1 (MQ=60)

                                                           CGCTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCAGGGAAGCTGTCCGGTCTtcgacccc                                    >  SRR4302535.3948416/1‑86 (MQ=60)

                                                             CTCATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTG                                  >  SRR4302535.252037/1‑94 (MQ=60)

                                                                ATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAAC                               >  SRR4302535.3753618/1‑94 (MQ=60)

                                                                ATGACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAAC                               <  SRR4302535.1088014/94‑1 (MQ=60)

                                                                   ACAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGT                            <  SRR4302535.3454043/94‑1 (MQ=60)

                                                                    CAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTT                           >  SRR4302535.710540/1‑94 (MQ=60)

                                                                    CAGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTT                           >  SRR4302535.29783/1‑94 (MQ=60)

                                                                     AGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTT                          <  SRR4302535.1853155/94‑1 (MQ=60)

                                                                     AGCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTT                          <  SRR4302535.502235/94‑1 (MQ=60)

                                                                      GCGCTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTA                         <  SRR4302535.3900942/94‑1 (MQ=60)

                                                                         CTTCCCGACCATGCCTCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGTGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCG                      <  SRR4302535.39602/94‑1 (MQ=60)

                                                                         CTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCG                      >  SRR4302535.1067467/1‑94 (MQ=60)

                                                                         CTTCCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCG                      >  SRR4302535.3543689/1‑94 (MQ=60)

                                                                          CTCCCGACCATGCCCCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGG                     <  SRR4302535.4351359/94‑1 (MQ=60)

                                                                            CCCGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTG                   <  SRR4302535.1668806/94‑1 (MQ=60)

                                                                            CCCGACCAGGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTG                   <  SRR4302535.1757076/94‑1 (MQ=60)

                                                                              CGACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCG                 >  SRR4302535.3924596/1‑94 (MQ=60)

                                                                               GACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGG                >  SRR4302535.3878918/1‑94 (MQ=60)

                                                                                ACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGT               >  SRR4302535.3048460/1‑94 (MQ=60)

                                                                                ACCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGT               <  SRR4302535.709082/94‑1 (MQ=60)

                                                                                 CCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTG              <  SRR4302535.3816420/94‑1 (MQ=60)

                                                                                 CCATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTG              <  SRR4302535.2899031/94‑1 (MQ=60)

                                                                                  CATGCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGG             >  SRR4302535.1036490/1‑94 (MQ=60)

                                                                                     GCCGCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACC          <  SRR4302535.801448/94‑1 (MQ=60)

                                                                                        GCCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGA       >  SRR4302535.4565492/1‑94 (MQ=60)

                                                                                         CCAGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGAT      >  SRR4302535.3474538/1‑94 (MQ=60)

                                                                                           AGCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATAT    <  SRR4302535.2761356/94‑1 (MQ=60)

                                                                                            GCCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATT   <  SRR4302535.752206/94‑1 (MQ=60)

                                                                                             CCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTT  <  SRR4302535.2939200/94‑1 (MQ=60)

ACATCCGGTTGTGGCCGTATCGGCCCGCACCGGCTGGGAACTGGATACGCTGGTCAGTGCGCTCATGACAGCGCTTCCCGACCATGCCGCCAGTCCCCTGATGACCCGACTGCAGGACGAGCTGCGCACGGAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTT  >  NC_000913/2068936‑2069122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`