Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,074,403 T→C V25A (GTG→GCG)  yeeS → putative DNA repair protein, RADC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,4030TC100.0% 353.0 / NA 114V25A (GTG→GCG) yeeSputative DNA repair protein, RADC family
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (53/61);  total (53/61)

Only 100 of 123 total aligned reads displayed.

GGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGA  >  NC_000913/2074313‑2074492
                                                                                          |                                                                                         
ggTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCgg                                                                                         >  1:937747/1‑93 (MQ=255)
 gTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGc                                                                                        >  1:4381397/1‑93 (MQ=255)
 gTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGc                                                                                        >  2:4371472/1‑93 (MQ=255)
  tAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGcc                                                                                       <  2:4143605/93‑1 (MQ=255)
  tAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGcc                                                                                       >  1:2803615/1‑93 (MQ=255)
   aaaGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCt                                                                                      <  2:4344118/93‑1 (MQ=255)
      gTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCa                                                                                   >  2:1159286/1‑93 (MQ=255)
        agagGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAcc                                                                                 <  2:4213612/93‑1 (MQ=255)
        agagGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAcc                                                                                 <  1:2258678/93‑1 (MQ=255)
        agagGAGAGAGGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAcc                                                                                 <  2:1749131/93‑1 (MQ=255)
         gaggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCt                                                                                <  1:1337627/93‑1 (MQ=255)
         gaggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCt                                                                                >  2:1538707/1‑93 (MQ=255)
          aggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTc                                                                               >  1:2607812/1‑93 (MQ=255)
            gagagaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCa                                                                             >  1:1236419/1‑93 (MQ=255)
             agagaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAc                                                                            >  1:172726/1‑93 (MQ=255)
              gagaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAcc                                                                           >  2:4559563/1‑93 (MQ=255)
               agaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAccc                                                                          >  2:3787277/1‑93 (MQ=255)
                gaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCg                                                                         >  2:4247106/1‑93 (MQ=255)
                gaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCg                                                                         >  2:4435450/1‑93 (MQ=255)
                 aTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGt                                                                        <  1:94191/93‑1 (MQ=255)
                   gACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgc                                                                      >  1:1674822/1‑93 (MQ=255)
                   gACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTAACCTCCACCCGTgc                                                                      >  1:3782001/1‑93 (MQ=255)
                    aCGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgca                                                                     <  1:4485928/93‑1 (MQ=255)
                      ccccGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgcagc                                                                   <  2:3609082/92‑1 (MQ=255)
                        ccGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCAc                                                                 <  1:3903625/93‑1 (MQ=255)
                         cGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACg                                                                >  1:1424147/1‑93 (MQ=255)
                           gCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACggg                                                              <  2:1149992/93‑1 (MQ=255)
                           gCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACggg                                                              >  2:4165958/1‑93 (MQ=255)
                              ggcgcAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCGCCCGTGCAGCACGGGAAt                                                           <  1:2208150/92‑1 (MQ=255)
                              aGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAt                                                           >  1:4318968/1‑93 (MQ=255)
                                 gcAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGc                                                        <  1:1747377/93‑1 (MQ=255)
                                 gcAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCAGGCGCGGCCTTCACCTCCACCCGTGCAGCACTGGAATggt                                                        >  2:1338670/1‑92 (MQ=255)
                                  cAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCt                                                       <  1:2595654/93‑1 (MQ=255)
                                   aGCCTCATTCAACGGGCCCTGAAAACCCTGGACCTCCATCTTCATGAACCCTGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTg                                                      >  1:2551532/1‑93 (MQ=255)
                                   aGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTg                                                      >  2:2061971/1‑93 (MQ=255)
                                    gCCTCATTCAACGGGCCCTGAAACCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGa                                                     <  2:1627378/93‑1 (MQ=255)
                                    gCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGa                                                     >  1:1006770/1‑93 (MQ=255)
                                    gCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGa                                                     <  1:3012320/93‑1 (MQ=255)
                                    gCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGa                                                     >  1:4350468/1‑93 (MQ=255)
                                     ccTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAt                                                    >  1:1995580/1‑93 (MQ=255)
                                     ccTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAt                                                    <  1:3985488/93‑1 (MQ=255)
                                      cTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAtt                                                   >  1:4065150/1‑93 (MQ=255)
                                       tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTTCCACCGTGCAGAACGGGCCTGGCTGCTTc                                                  >  2:1629738/1‑93 (MQ=255)
                                       tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                  <  1:2233608/93‑1 (MQ=255)
                                       tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                  >  2:1147533/1‑93 (MQ=255)
                                       tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                  >  2:3114608/1‑93 (MQ=255)
                                       tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                  <  1:148941/93‑1 (MQ=255)
                                        cATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCt                                                 >  1:2875237/1‑93 (MQ=255)
                                            cAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAAc                                             <  2:3442368/93‑1 (MQ=255)
                                             aaCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACa                                            >  2:2004164/1‑93 (MQ=255)
                                             aaCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACa                                            >  2:230365/1‑93 (MQ=255)
                                             aaCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACa                                            >  1:620420/1‑93 (MQ=255)
                                              aCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACAt                                           >  1:1221808/1‑93 (MQ=255)
                                               cGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTGACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATg                                          <  1:3305729/93‑1 (MQ=255)
                                                gggCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATgg                                         <  2:3611825/93‑1 (MQ=255)
                                                gggCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATgg                                         >  2:4411022/1‑93 (MQ=255)
                                                 ggCGCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGc                                        <  2:3530131/93‑1 (MQ=255)
                                                 ggCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGc                                        <  2:4164565/93‑1 (MQ=255)
                                                  gCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCg                                       <  1:388796/93‑1 (MQ=255)
                                                   cccTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCgg                                      <  1:3419168/93‑1 (MQ=255)
                                                    ccTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCggg                                     <  2:497239/93‑1 (MQ=255)
                                                     cTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGa                                    >  1:2041072/1‑93 (MQ=255)
                                                      tGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGAc                                   <  2:4006721/93‑1 (MQ=255)
                                                      tGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGAc                                   >  2:4632209/1‑93 (MQ=255)
                                                      tGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGAc                                   <  1:3127126/93‑1 (MQ=255)
                                                        aaaaCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTg                                 >  2:3060849/1‑93 (MQ=255)
                                                            cccTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGc                             <  1:1009751/93‑1 (MQ=255)
                                                            cccTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGc                             <  1:1979789/93‑1 (MQ=255)
                                                              cTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCgg                           >  1:1211758/1‑92 (MQ=255)
                                                                 gACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTgaa                        <  1:1642350/93‑1 (MQ=255)
                                                                  aCCGCCATCTTCATGAACCCGGCGCTGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTgaag                       <  2:787383/93‑1 (MQ=255)
                                                                  aCCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGCCGGGACTGGAGCGTgaag                       <  1:1841767/93‑1 (MQ=255)
                                                                    cGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGGgaagaa                     >  1:887118/1‑93 (MQ=255)
                                                                      ccATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGACCATGGCGGGGCTGGAGCGTGAAGAAtt                   >  1:4223854/1‑93 (MQ=37)
                                                                      ccATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAAtt                   <  1:3944684/93‑1 (MQ=255)
                                                                       cATCTTCATGACCCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTc                  <  2:2419340/93‑1 (MQ=255)
                                                                       cATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTc                  <  2:2902402/93‑1 (MQ=255)
                                                                       cATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTc                  <  2:2763123/93‑1 (MQ=255)
                                                                       cATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTc                  <  1:3381372/93‑1 (MQ=255)
                                                                        ctCTTCATGAACCCGGCGCGGCCTTCCCCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTcc                 <  1:4505921/92‑1 (MQ=255)
                                                                        aTCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTcc                 >  2:2915456/1‑93 (MQ=255)
                                                                           ttCATGACCCCGGCGCGCCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCggg              <  2:214934/93‑1 (MQ=255)
                                                                           ttCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCggg              <  1:982488/93‑1 (MQ=255)
                                                                           ttCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCggg              <  1:2953250/93‑1 (MQ=255)
                                                                            taaTGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGGAAGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGGAGGATTCCgggg             >  2:1037765/3‑92 (MQ=255)
                                                                            tCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCgggg             >  1:2320052/1‑92 (MQ=255)
                                                                             cATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTg            >  1:3452679/1‑93 (MQ=255)
                                                                              aTGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGc           <  1:3756763/93‑1 (MQ=255)
                                                                              aTGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGc           >  1:988744/1‑93 (MQ=255)
                                                                               tGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCt          <  2:3785612/93‑1 (MQ=255)
                                                                               tGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCt          >  1:195582/1‑93 (MQ=255)
                                                                                gAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTg         <  1:545004/93‑1 (MQ=255)
                                                                                 agcccGGCGCGGCCTTCACCTCCAGCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGt        <  2:1348439/91‑1 (MQ=255)
                                                                                 aaCCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGt        <  1:4595119/93‑1 (MQ=255)
                                                                                   ccccGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTAt      <  2:3913481/93‑1 (MQ=255)
                                                                                    ccGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATc     <  2:3181301/93‑1 (MQ=255)
                                                                                    ccGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATc     <  2:267572/93‑1 (MQ=255)
                                                                                     cGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTTGAGCGCGAAGAATTCCGGGTGCTGTATCt    >  1:4063255/1‑93 (MQ=255)
                                                                                     cGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCt    <  1:826116/93‑1 (MQ=255)
                                                                                       gcgcGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGa  <  1:1142905/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGA  >  NC_000913/2074313‑2074492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAA  >  NC_000913/2074311‑2074496
                                                                                            |                                                                                             
AAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCG                                                                                              <  SRR4302535.823606/94‑1 (MQ=60)
 AGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGG                                                                                             >  SRR4302535.944390/1‑94 (MQ=60)
 AGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGG                                                                                             <  SRR4302535.2274329/94‑1 (MQ=60)
  GGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGC                                                                                            >  SRR4302535.4411372/1‑94 (MQ=60)
   GTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCC                                                                                           >  SRR4302535.2823552/1‑94 (MQ=60)
    TAAAGTAGAGGAGAGATGACCCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCT                                                                                          <  SRR4302535.3344019/94‑1 (MQ=60)
      AAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTC                                                                                        >  SRR4302535.3806492/1‑94 (MQ=60)
          AGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCT                                                                                    <  SRR4302535.2274759/94‑1 (MQ=60)
           GAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTC                                                                                   <  SRR4302535.1347170/94‑1 (MQ=60)
           GAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTC                                                                                   >  SRR4302535.2626523/1‑94 (MQ=60)
             GGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCA                                                                                 >  SRR4302535.1245251/1‑94 (MQ=60)
              GAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAC                                                                                >  SRR4302535.173921/1‑94 (MQ=60)
                   ATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTG                                                                           <  SRR4302535.94861/94‑1 (MQ=60)
                    TGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGC                                                                          >  SRR4302535.1686948/1‑94 (MQ=60)
                    TGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTAACCTCCACCCGTGC                                                                          >  SRR4302535.3808097/1‑94 (MQ=60)
                      ACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAG                                                                        <  SRR4302535.4516721/94‑1 (MQ=60)
                          CCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACG                                                                    <  SRR4302535.3930365/94‑1 (MQ=60)
                          CCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACG                                                                    >  SRR4302535.1434316/1‑94 (MQ=60)
                               GAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAT                                                               >  SRR4302535.4348515/1‑94 (MQ=60)
                                GGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCGCCCGTGCAGCACGGGAATG                                                              <  SRR4302535.2223878/94‑1 (MQ=60)
                                   GCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCT                                                           <  SRR4302535.1760015/94‑1 (MQ=60)
                                    CAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCTCCATCTTCATGAACCCTGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTG                                                          >  SRR4302535.2569905/1‑94 (MQ=60)
                                    CAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTG                                                          <  SRR4302535.2614299/94‑1 (MQ=60)
                                     AGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGA                                                         >  SRR4302535.1013927/1‑94 (MQ=60)
                                     AGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGA                                                         >  SRR4302535.4380231/1‑94 (MQ=60)
                                      GCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAT                                                        <  SRR4302535.3033425/94‑1 (MQ=60)
                                      GCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAT                                                        >  SRR4302535.2009948/1‑94 (MQ=60)
                                      GCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAT                                                        <  SRR4302535.4352827/94‑1 (MQ=60)
                                       CCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATT                                                       >  SRR4302535.4092966/1‑94 (MQ=60)
                                       CCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATT                                                       <  SRR4302535.4012726/94‑1 (MQ=60)
                                       CCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATT                                                       <  SRR4302535.4560189/94‑1 (MQ=60)
                                         TCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCT                                                     <  SRR4302535.2249493/94‑1 (MQ=60)
                                         TCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCT                                                     <  SRR4302535.149979/94‑1 (MQ=60)
                                         TCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCT                                                     >  SRR4302535.2895605/1‑94 (MQ=60)
                                              CAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACA                                                >  SRR4302535.624733/1‑94 (MQ=60)
                                               AACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACAT                                               >  SRR4302535.1230560/1‑94 (MQ=60)
                                                 CGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTGACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGG                                             <  SRR4302535.3328732/94‑1 (MQ=60)
                                                    GCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGG                                          <  SRR4302535.391497/94‑1 (MQ=60)
                                                     CCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGG                                         <  SRR4302535.406595/94‑1 (MQ=60)
                                                     CCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGG                                         <  SRR4302535.3442949/94‑1 (MQ=60)
                                                      CCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGA                                        >  SRR4302535.2055772/1‑94 (MQ=60)
                                                        TGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACT                                      >  SRR4302535.2569137/1‑94 (MQ=60)
                                                        TGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACT                                      <  SRR4302535.3148904/94‑1 (MQ=60)
                                                              CCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCG                                <  SRR4302535.1016924/94‑1 (MQ=60)
                                                              CCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCG                                <  SRR4302535.1994044/94‑1 (MQ=60)
                                                               CCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGG                               >  SRR4302535.1220456/1‑94 (MQ=60)
                                                                   GACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAG                           <  SRR4302535.1654223/94‑1 (MQ=60)
                                                                    ACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGCCGGGACTGGAGCGTGAAGA                          <  SRR4302535.1855050/94‑1 (MQ=60)
                                                                     CCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGGGAAGAA                         >  SRR4302535.893400/1‑94 (MQ=60)
                                                                       GCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGACCATGGCGGGGCTGGAGCGTGAAGAATT                       >  SRR4302535.4252757/1‑94 (MQ=60)
                                                                        CCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTC                      <  SRR4302535.1953863/94‑1 (MQ=60)
                                                                        CCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTC                      <  SRR4302535.2970149/94‑1 (MQ=60)
                                                                        CCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTC                      <  SRR4302535.3971620/94‑1 (MQ=60)
                                                                         CATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCC                     <  SRR4302535.3404880/94‑1 (MQ=60)
                                                                          CTCTTCATGAACCCGGCGCGGCCTTCCCCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCG                    <  SRR4302535.4536852/94‑1 (MQ=60)
                                                                            CTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGGAGAATTCCGGG                  >  SRR4302535.2201066/1‑94 (MQ=60)
                                                                             TTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGT                 <  SRR4302535.2973998/94‑1 (MQ=60)
                                                                             TTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGT                 <  SRR4302535.989468/94‑1 (MQ=60)
                                                                             TTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGG                 >  SRR4302535.2336658/1‑94 (MQ=60)
                                                                              TCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTG                >  SRR4302535.3476678/1‑94 (MQ=60)
                                                                               CATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGC               >  SRR4302535.995767/1‑94 (MQ=60)
                                                                                ATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCT              >  SRR4302535.196927/1‑94 (MQ=60)
                                                                                ATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCT              <  SRR4302535.3782744/94‑1 (MQ=60)
                                                                                 TGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTG             <  SRR4302535.1623009/94‑1 (MQ=60)
                                                                                  GAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGT            <  SRR4302535.548812/94‑1 (MQ=60)
                                                                                  GAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGT            <  SRR4302535.1232602/94‑1 (MQ=60)
                                                                                   AACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTA           <  SRR4302535.4626386/94‑1 (MQ=60)
                                                                                   AACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTA           >  SRR4302535.932317/1‑94 (MQ=60)
                                                                                      CCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTTGAGCGCGAAGAATTCCGGGTGCTGTATCT        >  SRR4302535.4091062/1‑94 (MQ=60)
                                                                                       CGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTG       <  SRR4302535.831900/94‑1 (MQ=60)
                                                                                         GCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAA     <  SRR4302535.1151052/94‑1 (MQ=60)
                                                                                          CGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAAC    >  SRR4302535.2149664/1‑94 (MQ=60)
                                                                                          CGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAAC    <  SRR4302535.3748153/94‑1 (MQ=60)
                                                                                            CGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAA  >  SRR4302535.1146440/1‑94 (MQ=60)
                                                                                            |                                                                                             
AAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAA  >  NC_000913/2074311‑2074496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑