Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,080,323 T→C T85A (ACG→GCG)  dacD ← penicillin binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,080,3230TC100.0% 437.2 / NA 138T85A (ACG→GCG) dacDpenicillin binding protein 6b
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (75/63);  total (75/63)

Only 100 of 144 total aligned reads displayed.

CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAC  >  NC_000913/2080233‑2080412
                                                                                          |                                                                                         
ccTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCaa                                                                                         <  2:1715839/93‑1 (MQ=255)
ccTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCaa                                                                                         <  2:2898536/93‑1 (MQ=255)
ccTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCaa                                                                                         <  2:3960649/93‑1 (MQ=255)
ccTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAACATCGTCTGGCGCaa                                                                                         >  2:3545884/1‑93 (MQ=255)
 ctctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAt                                                                                        >  2:255410/1‑93 (MQ=255)
 ctctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAt                                                                                        <  2:3199868/93‑1 (MQ=255)
 ctctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAt                                                                                        >  1:3240774/1‑93 (MQ=255)
 ctctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAt                                                                                        >  2:4439922/1‑93 (MQ=255)
 ctctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAt                                                                                        <  1:3048675/93‑1 (MQ=255)
  tctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATg                                                                                       >  1:1125280/1‑93 (MQ=255)
  tctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATg                                                                                       >  1:588160/1‑93 (MQ=255)
  cctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATg                                                                                       <  1:2477790/92‑1 (MQ=255)
      tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGAt                                                                                   <  2:4317850/93‑1 (MQ=255)
        aaaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGa                                                                                 <  2:3762454/93‑1 (MQ=255)
         aaaaCATGAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATAGCGGCTGGCGCAATGCGATGAc                                                                                <  2:830670/93‑1 (MQ=255)
            aCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTAt                                                                             >  2:3651916/1‑93 (MQ=255)
               tCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGa                                                                          >  2:1972658/1‑93 (MQ=255)
                cAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCGCGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGAt                                                                         <  1:2730149/93‑1 (MQ=255)
                 aGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATa                                                                        <  2:3310441/93‑1 (MQ=255)
                 aGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATa                                                                        <  1:3901367/93‑1 (MQ=255)
                  gTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAg                                                                       <  2:3601140/93‑1 (MQ=255)
                  gTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAg                                                                       >  1:3924576/1‑93 (MQ=255)
                   tGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAgc                                                                      >  2:1098693/1‑93 (MQ=255)
                   tGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAgc                                                                      >  2:2957076/1‑93 (MQ=255)
                     aagaaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAgcgc                                                                    >  1:4090090/1‑93 (MQ=255)
                      agaaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGcgcg                                                                   <  1:2039431/93‑1 (MQ=255)
                      agaaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGcgcg                                                                   >  1:3580706/1‑93 (MQ=255)
                       gaaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGa                                                                  >  1:2666546/1‑93 (MQ=255)
                         aCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATc                                                                >  1:4248556/1‑93 (MQ=255)
                         aCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATc                                                                <  1:4682227/93‑1 (MQ=255)
                         aCCGACAAACACCGGATTATCTTTCGCCCACGCATAGCGCCCCACGGTGACAATATCGTCTGGCGCGATGCGATGACTATCGATAGGGCGATc                                                                >  2:2756445/1‑93 (MQ=255)
                          ccGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATcc                                                               >  2:3172382/1‑93 (MQ=255)
                            gACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCAc                                                             >  1:1882026/1‑93 (MQ=255)
                            gACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCAc                                                             >  2:3336101/1‑93 (MQ=255)
                              cAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGa                                                           >  2:1508677/1‑93 (MQ=255)
                                 acacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGCCaa                                                        >  2:3674417/1‑92 (MQ=255)
                                 acacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACAt                                                        >  1:674849/1‑93 (MQ=255)
                                  cacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATa                                                       <  2:3724045/93‑1 (MQ=255)
                                   acCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATaa                                                      >  2:685763/1‑93 (MQ=255)
                                    ccGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAc                                                     <  1:2115385/93‑1 (MQ=255)
                                    ccGGATTATCTTTCGCCCACGCATCGCGCCCCACGGGGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAc                                                     >  1:4088030/1‑93 (MQ=255)
                                     cGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAcc                                                    <  2:1512818/93‑1 (MQ=255)
                                      ggATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAccc                                                   <  2:628629/93‑1 (MQ=255)
                                      ggATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAccc                                                   >  2:3328566/1‑93 (MQ=255)
                                      ggATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAccc                                                   <  2:1057150/93‑1 (MQ=255)
                                       gATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCg                                                  >  2:351315/1‑93 (MQ=255)
                                       gATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCg                                                  >  1:1486117/1‑93 (MQ=255)
                                       gATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCg                                                  >  1:3740474/1‑93 (MQ=255)
                                       gATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCg                                                  <  1:3231409/93‑1 (MQ=255)
                                        aTTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGt                                                 >  2:2289783/1‑93 (MQ=255)
                                         ttATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtc                                                >  1:4518751/1‑93 (MQ=255)
                                         ttATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtc                                                >  1:4316896/1‑93 (MQ=255)
                                         ttATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtc                                                <  1:3706143/93‑1 (MQ=255)
                                         ttATCTTTCGCCCACGCACCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtc                                                <  1:984615/93‑1 (MQ=255)
                                          tATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtca                                               <  1:1395869/93‑1 (MQ=255)
                                             cTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtcatca                                            <  1:4358434/93‑1 (MQ=255)
                                              tttCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAg                                           >  2:2717180/1‑93 (MQ=255)
                                              tttCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAg                                           >  1:4319700/1‑93 (MQ=255)
                                              tttCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAg                                           >  2:3740922/1‑93 (MQ=255)
                                                tCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCt                                         <  2:2320831/93‑1 (MQ=255)
                                                 cGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCtt                                        <  1:4609936/93‑1 (MQ=255)
                                                 cGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCtt                                        <  2:1126830/93‑1 (MQ=255)
                                                 cGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCtt                                        >  1:504693/1‑93 (MQ=255)
                                                    ccACGCATCGCGCCCCACGGTGACAATATCGTCTGGGGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGt                                     >  2:3474037/1‑93 (MQ=255)
                                                    ccACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGt                                     <  2:1076870/93‑1 (MQ=255)
                                                     cACTCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTc                                    <  2:512861/93‑1 (MQ=255)
                                                     cACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTc                                    >  2:3365107/1‑93 (MQ=255)
                                                     cACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTc                                    >  1:4502573/1‑93 (MQ=255)
                                                      ccGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCa                                   <  1:925047/92‑1 (MQ=255)
                                                        gCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATTCGATGACTATCGATAGAGCGATCCACGACATAACCCGTCATCAGCTTTGTCaag                                 >  2:4286613/1‑91 (MQ=255)
                                                        gCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAgg                                 >  2:3353706/1‑93 (MQ=255)
                                                            cgcgCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATAAGCTTTGTCAGGCTgg                             >  1:2109616/1‑93 (MQ=255)
                                                                ccccACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCggg                         >  2:1441960/1‑93 (MQ=255)
                                                                 cccACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGa                        <  2:2029855/93‑1 (MQ=255)
                                                                 cccACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGa                        <  1:3265800/93‑1 (MQ=255)
                                                                  ccACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGAt                       <  2:161518/93‑1 (MQ=255)
                                                                  ccACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGAt                       <  1:1732161/93‑1 (MQ=255)
                                                                   cACGGTGACAATATCGTCTGGCGCAATGCTATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCATCTTTGTCAGGCTGGCGGGAtt                      <  1:3044439/93‑1 (MQ=255)
                                                                   cACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGAtt                      <  1:4146537/93‑1 (MQ=255)
                                                                    aCGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTg                     >  1:3875482/1‑93 (MQ=255)
                                                                     cGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTgc                    >  1:1478227/1‑93 (MQ=255)
                                                                     cGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTgc                    <  2:1803964/93‑1 (MQ=255)
                                                                       gTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTgcgc                  <  1:3563323/93‑1 (MQ=255)
                                                                          aCAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGt               >  2:2438697/1‑93 (MQ=255)
                                                                           cAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGtt              >  2:681499/1‑93 (MQ=255)
                                                                            aaTATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTg             >  1:1440735/1‑93 (MQ=255)
                                                                            aaTATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTg             >  1:3385970/1‑93 (MQ=255)
                                                                            aaTATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTg             >  2:1795788/1‑93 (MQ=255)
                                                                            aaTATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTg             >  2:4394607/1‑93 (MQ=255)
                                                                             atatCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGa            >  2:2407834/1‑93 (MQ=255)
                                                                              tatCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGAt           <  2:736223/93‑1 (MQ=255)
                                                                               atCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATg          <  2:3973884/93‑1 (MQ=255)
                                                                                tCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGc         <  2:4358412/93‑1 (MQ=255)
                                                                                tCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGc         >  1:2415033/1‑93 (MQ=255)
                                                                                 cGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCt        <  2:1960517/93‑1 (MQ=255)
                                                                                 cGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCt        <  1:3387018/93‑1 (MQ=255)
                                                                                      ggCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCAttc   >  1:4570502/1‑92 (MQ=255)
                                                                                      ggCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTa   <  1:724298/93‑1 (MQ=255)
                                                                                       gcgcAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAc  <  2:2097286/93‑1 (MQ=255)
                                                                                       gcgcAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAc  <  2:2097285/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAC  >  NC_000913/2080233‑2080412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGC  >  NC_000913/2080230‑2080416
                                                                                             |                                                                                             
CGCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGC                                                                                               >  SRR4302535.4175855/1‑94 (MQ=60)
   CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAT                                                                                            <  SRR4302535.4118410/94‑1 (MQ=60)
   CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAT                                                                                            >  SRR4302535.3263275/1‑94 (MQ=60)
    CTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATG                                                                                           >  SRR4302535.1133314/1‑94 (MQ=60)
    CTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATG                                                                                           >  SRR4302535.592282/1‑94 (MQ=60)
    CTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATG                                                                                           <  SRR4302535.3070032/94‑1 (MQ=60)
     CCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGC                                                                                          <  SRR4302535.2495660/94‑1 (MQ=60)
             AAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTA                                                                                  >  SRR4302535.1481704/1‑94 (MQ=60)
               ACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATC                                                                                <  SRR4302535.348143/94‑1 (MQ=60)
                  TCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGAT                                                                             <  SRR4302535.4034198/94‑1 (MQ=60)
                   CAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCGCGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATA                                                                            <  SRR4302535.2749639/94‑1 (MQ=60)
                    AGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAG                                                                           <  SRR4302535.3928091/94‑1 (MQ=60)
                    AGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAG                                                                           >  SRR4302535.3951393/1‑94 (MQ=60)
                      TGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCG                                                                         <  SRR4302535.871919/94‑1 (MQ=60)
                       GAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGC                                                                        >  SRR4302535.4118074/1‑94 (MQ=60)
                        AAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCG                                                                       >  SRR4302535.3605526/1‑94 (MQ=60)
                        AAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCG                                                                       <  SRR4302535.1981512/94‑1 (MQ=60)
                         AGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGA                                                                      <  SRR4302535.2054117/94‑1 (MQ=60)
                         AGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGA                                                                      >  SRR4302535.2685634/1‑94 (MQ=60)
                           AACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATC                                                                    >  SRR4302535.4277655/1‑94 (MQ=60)
                            ACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCC                                                                   <  SRR4302535.4713874/94‑1 (MQ=60)
                              CGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCAC                                                                 >  SRR4302535.1895591/1‑94 (MQ=60)
                              CGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCAC                                                                 >  SRR4302535.3803406/1‑94 (MQ=60)
                               GACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAAGATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACG                                                                <  SRR4302535.3320805/94‑1 (MQ=60)
                                 CAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGAC                                                              <  SRR4302535.2521191/94‑1 (MQ=60)
                                  AAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACA                                                             >  SRR4302535.2986454/1‑94 (MQ=60)
                                  AAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACA                                                             >  SRR4302535.537741/1‑94 (MQ=60)
                                   AACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACAT                                                            >  SRR4302535.679567/1‑94 (MQ=60)
                                      ACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGGGCGATCCACGACATAAC                                                         >  SRR4302535.4568812/1‑94 (MQ=60)
                                      ACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGGGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAC                                                         >  SRR4302535.4116003/1‑94 (MQ=60)
                                       CCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACC                                                        <  SRR4302535.2130653/94‑1 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      >  SRR4302535.3253673/1‑94 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      >  SRR4302535.1496752/1‑94 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      >  SRR4302535.3766362/1‑94 (MQ=60)
                                          GATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGT                                                     <  SRR4302535.3253815/94‑1 (MQ=60)
                                           ATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTC                                                    >  SRR4302535.4346427/1‑94 (MQ=60)
                                           ATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTC                                                    >  SRR4302535.4549725/1‑94 (MQ=60)
                                            TTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCA                                                   <  SRR4302535.3731808/94‑1 (MQ=60)
                                            TTATCTTTCGCCCACGCACCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCA                                                   <  SRR4302535.991608/94‑1 (MQ=60)
                                             TATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCAT                                                  <  SRR4302535.1405838/94‑1 (MQ=60)
                                                CTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAG                                               <  SRR4302535.4388271/94‑1 (MQ=60)
                                                CTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAG                                               >  SRR4302535.4349250/1‑94 (MQ=60)
                                                 TTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGC                                              >  SRR4302535.2260199/1‑94 (MQ=60)
                                                   TCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTT                                            >  SRR4302535.508221/1‑94 (MQ=60)
                                                    CGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTT                                           <  SRR4302535.4641258/94‑1 (MQ=60)
                                                     GCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTG                                          >  SRR4302535.4285947/1‑94 (MQ=60)
                                                       CCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTC                                        >  SRR4302535.4533492/1‑94 (MQ=60)
                                                         CCGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAG                                      <  SRR4302535.931597/94‑1 (MQ=60)
                                                              TCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATAAGCTTTGTCAGGCTGG                                 >  SRR4302535.2124830/1‑94 (MQ=60)
                                                                  GCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGG                             <  SRR4302535.3672250/94‑1 (MQ=60)
                                                                    CCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGAT                           <  SRR4302535.3288487/94‑1 (MQ=60)
                                                                     CCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATT                          <  SRR4302535.1744681/94‑1 (MQ=60)
                                                                      CACGGTGACAATATCGTCTGGCGCAATGCTATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCATCTTTGTCAGGCTGGCGGGATTG                         <  SRR4302535.3065764/94‑1 (MQ=60)
                                                                      CACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTG                         <  SRR4302535.4174929/94‑1 (MQ=60)
                                                                      CACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTG                         >  SRR4302535.3902105/1‑94 (MQ=60)
                                                                       CCGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGC                        <  SRR4302535.1737934/94‑1 (MQ=60)
                                                                       ACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGC                        >  SRR4302535.1488803/1‑94 (MQ=60)
                                                                          GTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCT                     <  SRR4302535.3588030/94‑1 (MQ=60)
                                                                            GACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGT                   >  SRR4302535.4497098/1‑94 (MQ=60)
                                                                              CAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTG                 >  SRR4302535.3409532/1‑94 (MQ=60)
                                                                              CAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTG                 >  SRR4302535.1451041/1‑94 (MQ=60)
                                                                                  ATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGC             >  SRR4302535.2432417/1‑94 (MQ=60)
                                                                                   TCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCT            <  SRR4302535.4651270/94‑1 (MQ=60)
                                                                                    CGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTC           <  SRR4302535.3410587/94‑1 (MQ=60)
                                                                                        TGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTC       >  SRR4302535.4601667/1‑94 (MQ=60)
                                                                                         GGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAC      <  SRR4302535.729381/94‑1 (MQ=60)
                                                                                           CGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCC    >  SRR4302535.2144321/1‑94 (MQ=60)
                                                                                           CGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCC    <  SRR4302535.4011300/94‑1 (MQ=60)
                                                                                             CAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGC  >  SRR4302535.4493119/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGC  >  NC_000913/2080230‑2080416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑