Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,081,054 A→G T93A (ACC→GCC)  sbcB → exonuclease I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,081,0540AG99.5% 651.2 / ‑5.3 210T93A (ACC→GCC) sbcBexonuclease I
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (89/120);  total (89/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 238 total aligned reads displayed.

GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT  >  NC_000913/2080964‑2081143
                                                                                          |                                                                                         
gCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgcc                                                                                         >  2:3884952/1‑93 (MQ=255)
   gTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgta                                                                                      >  1:4795105/1‑93 (MQ=255)
   gTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgta                                                                                      >  2:7630873/1‑93 (MQ=255)
    tATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtac                                                                                     >  1:1078825/1‑93 (MQ=255)
    tATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtac                                                                                     >  2:4967855/1‑93 (MQ=255)
    tATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtac                                                                                     >  1:1251009/1‑93 (MQ=255)
     attaattaCCGGTATTCCCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtacc                                                                                    <  1:1562580/93‑1 (MQ=255)
     attaattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtacc                                                                                    >  2:2522562/1‑93 (MQ=255)
      ttaattaCCTGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtaccg                                                                                   <  1:5742288/93‑1 (MQ=255)
      ttaattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtaccg                                                                                   >  2:6133507/1‑93 (MQ=255)
      ttaattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtaccg                                                                                   >  2:5350349/1‑93 (MQ=255)
      ttaattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTtgccgtaccg                                                                                   >  2:6520888/1‑93 (MQ=255)
        aattaCCGGTATTGCCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGaa                                                                                 <  1:3720440/93‑1 (MQ=255)
        aattaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGaa                                                                                 <  2:1328142/93‑1 (MQ=255)
         attaCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAg                                                                                >  1:1881140/1‑93 (MQ=255)
            aCCGGTATTCCCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGAcc                                                                             <  2:1727537/93‑1 (MQ=255)
             ccGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCt                                                                            >  2:6815656/1‑93 (MQ=255)
             ccGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCt                                                                            <  1:411095/93‑1 (MQ=255)
              cGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTg                                                                           <  1:3185511/93‑1 (MQ=255)
               ggTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGt                                                                          <  1:1064267/93‑1 (MQ=255)
                  aTTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTAtt                                                                       >  2:1507969/1‑93 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCTCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      <  2:3782018/93‑1 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      >  1:2546783/1‑93 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      <  1:7900205/93‑1 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      <  1:7285094/93‑1 (MQ=255)
                   ttACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTc                                                                      >  2:7116825/1‑93 (MQ=255)
                      ccccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTgg                                                                   <  2:4372474/93‑1 (MQ=255)
                        ccGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGc                                                                 >  1:7764007/1‑93 (MQ=255)
                          gCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTa                                                               >  1:6839224/1‑93 (MQ=255)
                            aGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTaca                                                             >  2:2830837/1‑93 (MQ=255)
                            aGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTaca                                                             >  1:4956406/1‑93 (MQ=255)
                             ggAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                            >  2:1433455/1‑93 (MQ=255)
                             ggAAGCACGGGCGAAAGGAGAAAACGAAACCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTacaa                                                            >  2:514112/1‑93 (MQ=255)
                                aGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTAcaacaa                                                         <  1:3437300/93‑1 (MQ=255)
                                   aCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgt                                                      <  2:2395402/93‑1 (MQ=255)
                                   aCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgt                                                      >  1:7832512/1‑93 (MQ=255)
                                   aCGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgt                                                      >  1:5596728/1‑93 (MQ=255)
                                    cGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAtgtg                                                     <  1:610927/93‑1 (MQ=255)
                                     gggCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGc                                                    <  1:4596556/93‑1 (MQ=255)
                                      ggCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCg                                                   >  1:966045/1‑93 (MQ=255)
                                       gCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGt                                                  <  2:7810084/93‑1 (MQ=255)
                                        cGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGtt                                                 >  1:7351719/1‑93 (MQ=255)
                                        cGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGtt                                                 <  2:4559946/93‑1 (MQ=255)
                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                                <  2:5571545/93‑1 (MQ=255)
                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                                >  2:4802655/1‑93 (MQ=255)
                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                                >  1:1654194/1‑93 (MQ=255)
                                         gAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGttt                                                <  2:5287890/93‑1 (MQ=255)
                                          aaaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTc                                               <  2:4734216/93‑1 (MQ=255)
                                          aaaGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTc                                               >  2:582458/1‑93 (MQ=255)
                                             ggAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgac                                            <  1:7514363/93‑1 (MQ=255)
                                             ggAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgac                                            >  2:1808430/1‑93 (MQ=255)
                                               agaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                          >  2:3685956/1‑93 (MQ=255)
                                               agaAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTcgacga                                          <  2:4820839/93‑1 (MQ=255)
                                                      gAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTc                                   >  2:3736974/1‑93 (MQ=255)
                                                       aaGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTca                                  >  2:3217508/1‑93 (MQ=255)
                                                       aaGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTca                                  <  1:43351/93‑1 (MQ=255)
                                                         gCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTcaca                                >  2:3413399/1‑93 (MQ=255)
                                                          ccGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCacac                               <  1:2997111/93‑1 (MQ=255)
                                                          ccGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCacac                               <  1:7138653/93‑1 (MQ=255)
                                                             cgTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCa                            <  2:6599402/93‑1 (MQ=255)
                                                              gTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCaa                           >  2:3003163/1‑93 (MQ=255)
                                                              gTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCaa                           >  1:537785/1‑93 (MQ=255)
                                                               tttGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAc                          >  2:1910122/1‑93 (MQ=255)
                                                               tttGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAAc                          <  1:3520314/93‑1 (MQ=255)
                                                                ttGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACa                         <  1:7114804/93‑1 (MQ=255)
                                                                ttGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTAATCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACa                         <  2:877374/93‑1 (MQ=255)
                                                                 tGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAt                        <  2:2100828/93‑1 (MQ=255)
                                                                  gccgccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtt                       <  2:752847/93‑1 (MQ=255)
                                                                  gccgccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtt                       <  1:2893043/93‑1 (MQ=255)
                                                                  gccgccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtt                       >  1:7807664/1‑93 (MQ=255)
                                                                    cgccCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtttt                     <  2:4326768/93‑1 (MQ=255)
                                                                      cccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtttttt                   >  1:2944928/1‑93 (MQ=255)
                                                                      cccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAtttttt                   >  1:3985396/1‑93 (MQ=255)
                                                                       ccGTATTCACTCTCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTa                  <  1:1777695/93‑1 (MQ=255)
                                                                       ccGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTa                  <  1:5143440/93‑1 (MQ=255)
                                                                        cGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAt                 <  2:4878233/93‑1 (MQ=255)
                                                                         gTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATc                >  1:1334493/1‑93 (MQ=255)
                                                                         gTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATc                >  1:243099/1‑93 (MQ=255)
                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg               <  2:722645/93‑1 (MQ=255)
                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg               >  1:6430724/1‑93 (MQ=255)
                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg               <  2:1147275/93‑1 (MQ=255)
                                                                          tATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCg               >  1:4540843/1‑93 (MQ=255)
                                                                             tCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTaa            <  1:6319721/93‑1 (MQ=255)
                                                                               ccTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt          <  2:7313552/92‑1 (MQ=255)
                                                                               aCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt          <  2:5986591/93‑1 (MQ=255)
                                                                               aCTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAtt          <  2:599683/93‑1 (MQ=255)
                                                                                 tCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTc        <  1:2121057/93‑1 (MQ=255)
                                                                                  cGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCt       <  2:1548376/93‑1 (MQ=255)
                                                                                   gCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTa      <  1:7487168/93‑1 (MQ=255)
                                                                                   gCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTa      <  2:4114831/93‑1 (MQ=255)
                                                                                    cTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAc     <  1:6685689/93‑1 (MQ=255)
                                                                                    cTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAc     <  1:870441/93‑1 (MQ=255)
                                                                                    cTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAc     >  1:5697148/1‑93 (MQ=255)
                                                                                     tttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACg    >  2:1319668/1‑93 (MQ=255)
                                                                                     tttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACg    >  2:2915507/1‑93 (MQ=255)
                                                                                     tttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACg    <  2:569070/93‑1 (MQ=255)
                                                                                     tttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACg    >  1:594972/1‑93 (MQ=255)
                                                                                      ttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGa   <  2:6566701/93‑1 (MQ=255)
                                                                                      ttttGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGa   <  1:3320345/93‑1 (MQ=255)
                                                                                      ttttGCCGTACCGAAGACCTGGATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGa   <  2:6826468/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT  >  NC_000913/2080964‑2081143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCT  >  NC_000913/2080961‑2081146
                                                                                             |                                                                                            
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTG                                                                                              <  SRR4302543.2159628/94‑1 (MQ=60)
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTG                                                                                              <  SRR4302543.1525252/94‑1 (MQ=60)
 GAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGC                                                                                             >  SRR4302543.6996668/1‑94 (MQ=60)
   GCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCG                                                                                           >  SRR4302543.7629676/1‑94 (MQ=60)
     CGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTA                                                                                         >  SRR4302543.2071549/1‑94 (MQ=60)
     CGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTA                                                                                         >  SRR4302543.4838931/1‑94 (MQ=60)
     CGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTA                                                                                         >  SRR4302543.653466/1‑94 (MQ=60)
      GTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTAC                                                                                        >  SRR4302543.1088522/1‑94 (MQ=60)
      GTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTAC                                                                                        >  SRR4302543.4435150/1‑94 (MQ=60)
      GTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTAC                                                                                        >  SRR4302543.1262227/1‑94 (MQ=60)
      GTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTAC                                                                                        >  SRR4302543.1088523/1‑94 (MQ=60)
       TATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACC                                                                                       >  SRR4302543.3786017/1‑94 (MQ=60)
        ATTAATTACCGGTATTCCCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCG                                                                                      <  SRR4302543.1576533/94‑1 (MQ=60)
        ATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTAACG                                                                                      >  SRR4302543.6395239/1‑94 (MQ=60)
         TTAATTACCTGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGA                                                                                     <  SRR4302543.5794637/94‑1 (MQ=60)
           AATTACCGGTATTGCCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAG                                                                                   <  SRR4302543.3753160/94‑1 (MQ=60)
           AATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAG                                                                                   <  SRR4302543.59662/94‑1 (MQ=60)
           AATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAG                                                                                   >  SRR4302543.572894/1‑94 (MQ=60)
           AATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAG                                                                                   >  SRR4302543.1898044/1‑94 (MQ=60)
             TTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGAC                                                                                 <  SRR4302543.932676/94‑1 (MQ=60)
             TTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGAC                                                                                 >  SRR4302543.3021090/1‑94 (MQ=60)
             TTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGAC                                                                                 <  SRR4302543.4838736/94‑1 (MQ=60)
               TCCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCT                                                                               <  SRR4302543.6860085/94‑1 (MQ=60)
               ACCGGTATTACCCCGCAGGAAGCATGGGCGAAAGGAGAAAACGAAGACGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTTCCGAAGACCT                                                                               >  SRR4302543.5664691/1‑94 (MQ=60)
                CCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTG                                                                              <  SRR4302543.6046203/94‑1 (MQ=60)
                CCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTG                                                                              <  SRR4302543.1262975/94‑1 (MQ=60)
                CCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTG                                                                              <  SRR4302543.414899/94‑1 (MQ=60)
                 CGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGT                                                                             <  SRR4302543.3214242/94‑1 (MQ=60)
                  GGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTA                                                                            <  SRR4302543.1073826/94‑1 (MQ=60)
                    TATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCGGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATT                                                                          >  SRR4302543.2387059/1‑94 (MQ=60)
                     ATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTC                                                                         >  SRR4302543.2570020/1‑94 (MQ=60)
                      TTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCT                                                                        <  SRR4302543.7974468/94‑1 (MQ=60)
                      TTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCT                                                                        <  SRR4302543.7355436/94‑1 (MQ=60)
                         CCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGG                                                                     >  SRR4302543.1614122/1‑94 (MQ=60)
                          CCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGC                                                                    <  SRR4302543.2946353/94‑1 (MQ=60)
                          CCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGC                                                                    >  SRR4302543.7837274/1‑94 (MQ=60)
                          CCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGC                                                                    <  SRR4302543.172658/94‑1 (MQ=60)
                           CCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCT                                                                   <  SRR4302543.172558/94‑1 (MQ=60)
                            CGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTA                                                                  >  SRR4302543.6903779/1‑94 (MQ=60)
                            CGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTA                                                                  >  SRR4302543.1482203/1‑94 (MQ=60)
                              CAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACA                                                                >  SRR4302543.319506/1‑94 (MQ=60)
                              CAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACA                                                                >  SRR4302543.5001532/1‑94 (MQ=60)
                               AGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAA                                                               >  SRR4302543.5780576/1‑94 (MQ=60)
                                GGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAAC                                                              <  SRR4302543.7882472/94‑1 (MQ=60)
                                GGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAAC                                                              <  SRR4302543.3926324/94‑1 (MQ=60)
                                 GAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACA                                                             >  SRR4302543.6961201/1‑94 (MQ=60)
                                   AGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAT                                                           >  SRR4302543.1180946/1‑94 (MQ=60)
                                   AGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAT                                                           <  SRR4302543.3468066/94‑1 (MQ=60)
                                   AGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAAT                                                           <  SRR4302543.6062962/94‑1 (MQ=60)
                                    GCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATG                                                          <  SRR4302543.7887810/94‑1 (MQ=60)
                                     CACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGT                                                         >  SRR4302543.336650/1‑94 (MQ=60)
                                     CACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGT                                                         >  SRR4302543.5647718/1‑94 (MQ=60)
                                     CACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGT                                                         >  SRR4302543.7906254/1‑94 (MQ=60)
                                       CGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGC                                                       <  SRR4302543.616506/94‑1 (MQ=60)
                                        GGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCG                                                      >  SRR4302543.7370312/1‑94 (MQ=60)
                                        GGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCG                                                      >  SRR4302543.974526/1‑94 (MQ=60)
                                        GGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCG                                                      <  SRR4302543.4638296/94‑1 (MQ=60)
                                         GGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGT                                                     >  SRR4302543.5226172/1‑94 (MQ=60)
                                          GCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTT                                                    >  SRR4302543.7422434/1‑94 (MQ=60)
                                           AGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT                                                   >  SRR4302543.1668997/1‑94 (MQ=60)
                                             AAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCG                                                 <  SRR4302543.80729/94‑1 (MQ=60)
                                               AGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGAC                                               >  SRR4302543.7965608/1‑94 (MQ=60)
                                                GGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACG                                              <  SRR4302543.7586125/94‑1 (MQ=60)
                                                  AGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGAC                                            <  SRR4302543.1993069/94‑1 (MQ=60)
                                                  AGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGAC                                            >  SRR4302543.6574082/1‑94 (MQ=60)
                                                  AGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGAC                                            <  SRR4302543.7459489/94‑1 (MQ=60)
                                                   GAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACG                                           <  SRR4302543.7292186/94‑1 (MQ=60)
                                                   GAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACG                                           <  SRR4302543.4442792/94‑1 (MQ=60)
                                                   GAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACG                                           >  SRR4302543.6207964/1‑94 (MQ=60)
                                                        CGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTC                                      <  SRR4302543.3423504/94‑1 (MQ=60)
                                                          AAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCAC                                    <  SRR4302543.43749/94‑1 (MQ=60)
                                                             CCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACG                                 <  SRR4302543.7208018/94‑1 (MQ=60)
                                                             CCGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACG                                 <  SRR4302543.3024299/94‑1 (MQ=60)
                                                              CGCGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGC                                >  SRR4302543.7678827/1‑94 (MQ=60)
                                                                CGTTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAA                              >  SRR4302543.542767/1‑94 (MQ=60)
                                                                  TTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACA                            >  SRR4302543.459701/1‑94 (MQ=60)
                                                                  TTTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACA                            <  SRR4302543.3551730/94‑1 (MQ=60)
                                                                   TTGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACAT                           <  SRR4302543.7183919/94‑1 (MQ=60)
                                                                    TGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATT                          >  SRR4302543.3643976/1‑94 (MQ=60)
                                                                    TGCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATT                          >  SRR4302543.7881260/1‑94 (MQ=60)
                                                                     GCCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTT                         <  SRR4302543.2919357/94‑1 (MQ=60)
                                                                      CCGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTT                        >  SRR4302543.1808843/1‑94 (MQ=60)
                                                                       CGCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTT                       >  SRR4302543.5317578/1‑94 (MQ=60)
                                                                        GCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTT                      <  SRR4302543.4044166/94‑1 (MQ=60)
                                                                        GCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTT                      >  SRR4302543.4020770/1‑94 (MQ=60)
                                                                        GCCCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTT                      >  SRR4302543.2971667/1‑94 (MQ=60)
                                                                          CCGTATTCACTCTCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAT                    <  SRR4302543.1793576/94‑1 (MQ=60)
                                                                          CCGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTAT                    <  SRR4302543.5189941/94‑1 (MQ=60)
                                                                           CGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATC                   >  SRR4302543.1346386/1‑94 (MQ=60)
                                                                           CGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATC                   >  SRR4302543.245204/1‑94 (MQ=60)
                                                                           CGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATC                   >  SRR4302543.3522321/1‑94 (MQ=60)
                                                                           CGTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATC                   <  SRR4302543.1324535/94‑1 (MQ=60)
                                                                            GTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCG                  >  SRR4302543.4582189/1‑94 (MQ=60)
                                                                            GTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCG                  >  SRR4302543.4709823/1‑94 (MQ=60)
                                                                            GTATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCG                  >  SRR4302543.6491316/1‑94 (MQ=60)
                                                                            GTATTCACTCGCTTTTTGCCGTACCGAAGACCTGGATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCG                  <  SRR4302543.3046539/94‑1 (MQ=60)
                                                                             TATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGT                 <  SRR4302543.4241176/94‑1 (MQ=60)
                                                                             TATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGT                 >  SRR4302543.5020231/1‑94 (MQ=60)
                                                                              ATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGTGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTA                <  SRR4302543.6909424/94‑1 (MQ=60)
                                                                              ATTCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTA                >  SRR4302543.2528415/1‑94 (MQ=60)
                                                                                TCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAT              <  SRR4302543.7843688/94‑1 (MQ=60)
                                                                                TCACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAAT              <  SRR4302543.6379151/94‑1 (MQ=60)
                                                                                 CACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT             <  SRR4302543.755832/94‑1 (MQ=60)
                                                                                 CACTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATT             <  SRR4302543.283032/94‑1 (MQ=60)
                                                                                  TCTCGCTTTTTGCAGTTCCGAAGACCTGTATTCTGGGCTACATCAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTT            <  SRR4302543.3249646/94‑1 (MQ=60)
                                                                                   CTCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTC           >  SRR4302543.909062/1‑94 (MQ=60)
                                                                                   CTCGCTTCTTGCCGTACCGAAGACCTGTATTCTGGAATACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTC           <  SRR4302543.2691028/94‑1 (MQ=60)
                                                                                    TCGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCT          <  SRR4302543.2140047/94‑1 (MQ=60)
                                                                                     CGCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTA         >  SRR4302543.7400438/1‑94 (MQ=60)
                                                                                      GCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAC        <  SRR4302543.7558810/94‑1 (MQ=60)
                                                                                      GCTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTAC        >  SRR4302543.5749122/1‑94 (MQ=60)
                                                                                       CTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG       <  SRR4302543.878207/94‑1 (MQ=60)
                                                                                       CTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG       >  SRR4302543.600430/1‑94 (MQ=60)
                                                                                       CTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG       <  SRR4302543.6748805/94‑1 (MQ=60)
                                                                                       CTTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG       >  SRR4302543.5514833/1‑94 (MQ=60)
                                                                                       CTTGTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACG       <  SRR4302543.7197078/94‑1 (MQ=60)
                                                                                        TTTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGA      >  SRR4302543.4943672/1‑94 (MQ=60)
                                                                                         TTTTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGAT     <  SRR4302543.3350305/94‑1 (MQ=60)
                                                                                           TTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCC   >  SRR4302543.1479170/1‑94 (MQ=60)
                                                                                           TTGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCC   <  SRR4302543.1921071/94‑1 (MQ=60)
                                                                                            TGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCT  <  SRR4302543.1918474/94‑1 (MQ=60)
                                                                                            TGCCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCT  <  SRR4302543.96909/94‑1 (MQ=60)
                                                                                             |                                                                                            
GGAGCCGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGACGACGAAGTCACACGCAACATTTTTTATCGTAATTTCTACGATCCT  >  NC_000913/2080961‑2081146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑