Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,087,857 T→C intergenic (‑95/‑161) yefM ← / → hisL hypothetical protein/his operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,087,8570TC98.8% 772.5 / ‑4.2 246intergenic (‑95/‑161)yefM/hisLhypothetical protein/his operon leader peptide
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (131/112);  total (133/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

CAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAG  >  NC_000913/2087767‑2087946
                                                                                          |                                                                                         
cAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGgcgc                                                                                         <  2:7914691/93‑1 (MQ=255)
 aaTGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGcc                                                                                        >  2:3865704/1‑93 (MQ=255)
 aaTGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGcc                                                                                        >  1:1890666/1‑93 (MQ=255)
 aaTGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGcc                                                                                        >  1:3688159/1‑93 (MQ=255)
    gatgaGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATc                                                                                     >  1:925372/1‑93 (MQ=255)
       gaGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGc                                                                                  <  1:7751048/93‑1 (MQ=255)
       gaGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGc                                                                                  >  2:2904766/1‑93 (MQ=255)
       gaGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGc                                                                                  >  1:4146264/1‑93 (MQ=255)
          cGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTaa                                                                               <  1:5424447/93‑1 (MQ=255)
          cGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTaa                                                                               >  1:2893144/1‑93 (MQ=255)
           gTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAag                                                                              >  1:3480084/1‑93 (MQ=255)
            ttaattaaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAaga                                                                             <  2:3721021/93‑1 (MQ=255)
            ttaattaaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAaga                                                                             <  1:1776847/93‑1 (MQ=255)
                ttaaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGAc                                                                         >  2:3224458/1‑93 (MQ=255)
                ttaaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGAc                                                                         >  2:6129920/1‑93 (MQ=255)
                ttaaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGAc                                                                         <  2:5502444/93‑1 (MQ=255)
                 taaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACa                                                                        >  1:7502985/1‑93 (MQ=255)
                 taaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACa                                                                        <  2:7644377/93‑1 (MQ=255)
                  aaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAg                                                                       >  2:6040489/1‑93 (MQ=255)
                  aaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAg                                                                       >  2:6208385/1‑93 (MQ=255)
                   aCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAgc                                                                      <  2:3489136/93‑2 (MQ=255)
                   aCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGt                                                                      >  1:4718465/1‑93 (MQ=255)
                    cTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTc                                                                     >  1:5704671/1‑93 (MQ=255)
                    cTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTc                                                                     >  1:3791003/1‑93 (MQ=255)
                     tatttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTcc                                                                    >  1:6596901/1‑93 (MQ=255)
                     tatttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTcc                                                                    <  1:7118005/93‑1 (MQ=255)
                     tatttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTcc                                                                    <  1:1316382/93‑1 (MQ=255)
                        ttattaATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCctct                                                                 <  1:152736/93‑1 (MQ=255)
                         tattaATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCtt                                                                <  1:2704783/93‑1 (MQ=255)
                         tattaATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCGCtt                                                                >  1:494173/1‑93 (MQ=255)
                         tattaATTAGTTTGTAGATCAAGCTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCtt                                                                <  2:5661439/93‑1 (MQ=255)
                          attaattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTa                                                               <  1:1109056/93‑1 (MQ=255)
                           ttaattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGTCAGTCCTCTTAg                                                              <  1:5042756/93‑1 (MQ=255)
                            taattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGc                                                             >  1:5787931/1‑93 (MQ=255)
                            taattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGc                                                             >  2:5176603/1‑93 (MQ=255)
                              attaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGccc                                                           >  2:4319774/1‑93 (MQ=255)
                              attaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGccc                                                           <  1:6611536/93‑1 (MQ=255)
                               ttaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCTATCAGCTAAGAGGACAGCCCTCTTAGcccc                                                          >  2:7728163/1‑93 (MQ=255)
                               ttaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGcccc                                                          >  1:2263643/1‑93 (MQ=255)
                                  gTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCcctc                                                       >  2:5638964/1‑93 (MQ=255)
                                   tttGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGGCAGTCCTCTTAGCCCcctcc                                                      >  1:4005376/1‑93 (MQ=255)
                                   tttGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCcctcc                                                      >  1:1649020/1‑93 (MQ=255)
                                   tttGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCcctcc                                                      <  2:4067087/93‑1 (MQ=255)
                                   tttGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCcctcc                                                      >  1:5256385/1‑93 (MQ=255)
                                   tttGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAACCCcctcc                                                      >  1:5576832/1‑93 (MQ=255)
                                     tGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCtt                                                    >  2:1392789/1‑93 (MQ=255)
                                      gTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGCCCTCTTAGCCCCCTCCttt                                                   >  2:3515292/1‑93 (MQ=255)
                                       tAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTc                                                  >  1:6421748/1‑93 (MQ=255)
                                        aGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTcc                                                 >  2:5763022/1‑93 (MQ=255)
                                         gATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTccc                                                >  1:4223089/1‑93 (MQ=255)
                                         gATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTccc                                                <  1:976714/93‑1 (MQ=255)
                                           tCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCg                                              >  1:6924118/1‑93 (MQ=255)
                                              tggTATTGTCAGTGAGTCGAAAATCCAGGCTTCGCTATTTTTGGCGCCAGCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCTCCGCtc                                           >  2:7020186/2‑93 (MQ=255)
                                              aGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtc                                           >  2:1954007/1‑93 (MQ=255)
                                                gTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcat                                         <  1:3137559/93‑1 (MQ=255)
                                                gTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcat                                         >  1:4840101/1‑93 (MQ=255)
                                                gTATTGTCAGTGAGACGAAAAGCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcat                                         <  1:6170830/93‑1 (MQ=255)
                                                 tATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcatt                                        >  2:265972/1‑93 (MQ=255)
                                                 tATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcatt                                        >  1:3263516/1‑93 (MQ=255)
                                                 tATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcatt                                        <  1:860277/93‑1 (MQ=255)
                                                  aTTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAG‑CCCCTCCTTTCCCCGCtcattca                                      >  2:5058223/1‑93 (MQ=255)
                                                   ttGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcattca                                      <  1:6253502/93‑1 (MQ=255)
                                                   ttGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcattca                                      >  2:4015728/1‑93 (MQ=255)
                                                   ttGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGCCCTCTTAGCCCCCTCCTTTCCCCGCtcattca                                      <  1:4344423/93‑1 (MQ=255)
                                                    tGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcattcat                                     >  1:4496643/1‑93 (MQ=255)
                                                      tCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTa                                   >  2:7373296/1‑93 (MQ=255)
                                                      tCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTa                                   <  1:7851838/93‑1 (MQ=255)
                                                        aGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTaaa                                 <  2:1717251/93‑1 (MQ=255)
                                                         gTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAAc                                <  1:6929897/93‑1 (MQ=255)
                                                          tGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACa                               <  1:729937/93‑1 (MQ=255)
                                                           gagaCGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACaa                              <  2:1777806/93‑1 (MQ=255)
                                                             gaCGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAAt                            >  1:3928390/1‑93 (MQ=255)
                                                                gAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCa                         <  2:5402059/93‑1 (MQ=255)
                                                                 aaaaTCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCAt                        >  1:5110528/1‑93 (MQ=255)
                                                                 aaaaTCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCAt                        >  2:1009101/1‑93 (MQ=255)
                                                                 aaaaTCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCAt                        <  1:4578482/93‑1 (MQ=255)
                                                                  aaaTCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCAtt                       >  2:6315968/1‑93 (MQ=255)
                                                                  aaaTCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCAtt                       <  2:6787956/93‑1 (MQ=255)
                                                                  aaaTCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCAtt                       <  1:109961/93‑1 (MQ=255)
                                                                  aaaTCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCCCCTTTCCCCGCTCATTCATTAAACAAATCCAtt                       >  1:7614896/1‑93 (MQ=255)
                                                                   aaTCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTg                      >  2:1521639/1‑93 (MQ=255)
                                                                      ccAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCa                   <  1:6313452/93‑1 (MQ=255)
                                                                      ccAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCa                   >  1:2177084/1‑93 (MQ=255)
                                                                      ccAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGCCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCa                   <  2:2824031/93‑1 (MQ=255)
                                                                       cAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCAt                  <  1:5656375/93‑1 (MQ=255)
                                                                       cAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCAt                  >  2:5577301/1‑93 (MQ=255)
                                                                         ggCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATaa                >  1:441870/1‑93 (MQ=255)
                                                                           cTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATaaaa              >  2:1100998/1‑93 (MQ=255)
                                                                           cTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGCCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATaaaa              <  2:2388816/93‑1 (MQ=255)
                                                                              cGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAatat           >  2:4364910/1‑93 (MQ=255)
                                                                               gCTATTTTTGGCGCCCTCAGCTAAGAGGACAGTCCTCTTCGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTTCCATAAAatata          >  1:7060886/1‑93 (MQ=255)
                                                                               gCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAatata          >  1:6017950/1‑93 (MQ=255)
                                                                                cTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAatatat         >  2:187482/1‑93 (MQ=255)
                                                                                cTATTTTTGCCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCACTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAatatat         <  2:4646288/93‑1 (MQ=255)
                                                                                 tATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAAtatata        >  2:5877157/1‑93 (MQ=255)
                                                                                   tttttGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATaaa      <  2:4858670/93‑1 (MQ=255)
                                                                                   tttttGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATaaa      <  2:3228321/93‑1 (MQ=255)
                                                                                     tttGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATaaaaa    >  2:2220348/1‑93 (MQ=255)
                                                                                     tgtGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATaaaaa    <  1:3429083/91‑1 (MQ=255)
                                                                                      ttGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATaaaaaa   <  2:1258481/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAG  >  NC_000913/2087767‑2087946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCT  >  NC_000913/2087764‑2087950
                                                                                             |                                                                                             
GTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGC                                                                                               <  SRR4302543.3290223/94‑1 (MQ=60)
GTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGC                                                                                               <  SRR4302543.2910697/94‑1 (MQ=60)
   CAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCC                                                                                            >  SRR4302543.3720573/1‑94 (MQ=60)
   CAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCC                                                                                            >  SRR4302543.1907666/1‑94 (MQ=60)
      TGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATC                                                                                         >  SRR4302543.933464/1‑94 (MQ=60)
        ATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAG                                                                                       <  SRR4302543.991669/94‑1 (MQ=60)
        ATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAG                                                                                       >  SRR4302543.4907755/1‑94 (MQ=60)
         TGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGC                                                                                      >  SRR4302543.4183738/1‑94 (MQ=60)
          GAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCT                                                                                     <  SRR4302543.7824240/94‑1 (MQ=60)
          GAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCT                                                                                     <  SRR4302543.4879227/94‑1 (MQ=60)
          GAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCT                                                                                     >  SRR4302543.4490385/1‑94 (MQ=60)
            GCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAA                                                                                   >  SRR4302543.2919458/1‑94 (MQ=60)
             CGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAG                                                                                  <  SRR4302543.5473751/94‑1 (MQ=60)
             CGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAG                                                                                  <  SRR4302543.1317144/94‑1 (MQ=60)
             CGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAG                                                                                  >  SRR4302543.3511254/1‑94 (MQ=60)
               TTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAG                                                                                <  SRR4302543.1792721/94‑1 (MQ=60)
               TTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAG                                                                                <  SRR4302543.7437386/94‑1 (MQ=60)
               TTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAG                                                                                <  SRR4302543.7232164/94‑1 (MQ=60)
                TAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGG                                                                               <  SRR4302543.3635095/94‑1 (MQ=60)
                TAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGG                                                                               <  SRR4302543.399559/94‑1 (MQ=60)
                  ATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGAC                                                                             <  SRR4302543.6920331/94‑1 (MQ=60)
                  ATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGAC                                                                             <  SRR4302543.4305563/94‑1 (MQ=60)
                   TTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACA                                                                            >  SRR4302543.7574716/1‑94 (MQ=60)
                   TTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACA                                                                            <  SRR4302543.3303930/94‑1 (MQ=60)
                   TTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACA                                                                            >  SRR4302543.4241022/1‑94 (MQ=60)
                   TTAACTATTTATTAATTAGTTTGTACATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACA                                                                            >  SRR4302543.6624826/1‑94 (MQ=60)
                    TAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAG                                                                           >  SRR4302543.1386659/1‑94 (MQ=60)
                     AACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGT                                                                          >  SRR4302543.4761578/1‑94 (MQ=60)
                     AACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGT                                                                          <  SRR4302543.7635715/94‑1 (MQ=60)
                     AACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGT                                                                          >  SRR4302543.78984/1‑94 (MQ=60)
                      ACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTC                                                                         <  SRR4302543.4057016/94‑1 (MQ=60)
                      ACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTC                                                                         >  SRR4302543.3824404/1‑94 (MQ=60)
                      ACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTC                                                                         >  SRR4302543.5756702/1‑94 (MQ=60)
                       CTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCC                                                                        <  SRR4302543.3433576/94‑1 (MQ=60)
                       CTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCC                                                                        >  SRR4302543.6659069/1‑94 (MQ=60)
                       CTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCC                                                                        >  SRR4302543.7234000/1‑94 (MQ=60)
                       CTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCC                                                                        >  SRR4302543.7954997/1‑94 (MQ=60)
                        TATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCT                                                                       >  SRR4302543.7180112/1‑94 (MQ=60)
                        TATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCT                                                                       <  SRR4302543.7187152/94‑1 (MQ=60)
                        TATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCT                                                                       >  SRR4302543.1589234/1‑94 (MQ=60)
                        TATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCT                                                                       <  SRR4302543.1328079/94‑1 (MQ=60)
                         ATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTC                                                                      >  SRR4302543.6429094/1‑94 (MQ=60)
                         ATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTC                                                                      >  SRR4302543.7745528/1‑94 (MQ=60)
                          TTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCT                                                                     <  SRR4302543.3547994/94‑1 (MQ=60)
                           TTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTT                                                                    >  SRR4302543.5625853/1‑94 (MQ=60)
                           TTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTT                                                                    >  SRR4302543.5966072/1‑94 (MQ=60)
                           TTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTT                                                                    <  SRR4302543.154060/94‑1 (MQ=60)
                           TTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCGCTT                                                                    >  SRR4302543.498745/1‑94 (MQ=60)
                            TATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTA                                                                   >  SRR4302543.1698670/1‑94 (MQ=60)
                            TATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTA                                                                   <  SRR4302543.2729328/94‑1 (MQ=60)
                            TATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTA                                                                   >  SRR4302543.5071562/1‑94 (MQ=60)
                             ATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAG                                                                  >  SRR4302543.7122989/1‑94 (MQ=60)
                             ATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAG                                                                  <  SRR4302543.1119062/94‑1 (MQ=60)
                             ATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAG                                                                  >  SRR4302543.6243914/1‑94 (MQ=60)
                              TTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGTCAGTCCTCTTAGC                                                                 <  SRR4302543.5088614/94‑1 (MQ=60)
                              TTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGC                                                                 >  SRR4302543.5840595/1‑94 (MQ=60)
                               TAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCC                                                                >  SRR4302543.4770923/1‑94 (MQ=60)
                                 ATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCC                                                              <  SRR4302543.6673855/94‑1 (MQ=60)
                                 ATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCC                                                              >  SRR4302543.2284300/1‑94 (MQ=60)
                                  TTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCC                                                             >  SRR4302543.821067/1‑94 (MQ=60)
                                   TAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCT                                                            <  SRR4302543.2931970/94‑1 (MQ=60)
                                    AGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTC                                                           >  SRR4302543.5184621/1‑94 (MQ=60)
                                     GTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGGCAGTCCTCTTAGCCCCCTCC                                                          >  SRR4302543.4041115/1‑94 (MQ=60)
                                     GTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCC                                                          >  SRR4302543.1663771/1‑94 (MQ=60)
                                     GTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCC                                                          >  SRR4302543.5303793/1‑94 (MQ=60)
                                     GTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAACCCCCTCC                                                          >  SRR4302543.5627600/1‑94 (MQ=60)
                                      TTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCT                                                         >  SRR4302543.542601/1‑94 (MQ=60)
                                        TGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTT                                                       >  SRR4302543.2125470/1‑94 (MQ=60)
                                        TGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTT                                                       <  SRR4302543.515703/94‑1 (MQ=60)
                                         GTAGATCAAGGTATTGTCAGTGAGNCGANANTCCAGNCTTCGCTATTTTTGGCGCCATCNGCTAAGANNANANNCCTCNTANCNNCNTNCNNTN                                                      >  SRR4302543.7460805/1‑94 (MQ=60)
                                         GTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTC                                                      >  SRR4302543.6482256/1‑94 (MQ=60)
                                          TAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCC                                                     <  SRR4302543.5714197/94‑1 (MQ=60)
                                           AGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCC                                                    >  SRR4302543.4261386/1‑94 (MQ=60)
                                            GATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCC                                                   <  SRR4302543.985329/94‑1 (MQ=60)
                                             ATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCG                                                  >  SRR4302543.6989224/1‑94 (MQ=60)
                                             ATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCG                                                  >  SRR4302543.6989223/1‑94 (MQ=60)
                                             ATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCG                                                  <  SRR4302543.203553/94‑1 (MQ=60)
                                                 AGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCA                                              <  SRR4302543.6207507/94‑1 (MQ=60)
                                                 AGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCA                                              <  SRR4302543.2982533/94‑1 (MQ=60)
                                                  GGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCAT                                             >  SRR4302543.4884238/1‑94 (MQ=60)
                                                  GGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCAT                                             >  SRR4302543.3766448/1‑94 (MQ=60)
                                                  AGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCAT                                             <  SRR4302543.2787677/94‑1 (MQ=60)
                                                   GTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATT                                            <  SRR4302543.3165779/94‑1 (MQ=60)
                                                   GTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATT                                            >  SRR4302543.6668070/1‑94 (MQ=60)
                                                   GTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATT                                            >  SRR4302543.3293012/1‑94 (MQ=60)
                                                   GTATTGTCAGTGAGACGAAAAGCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATT                                            <  SRR4302543.6229201/94‑1 (MQ=60)
                                                    TATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTC                                           <  SRR4302543.4617537/94‑1 (MQ=60)
                                                    TATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTC                                           <  SRR4302543.867942/94‑1 (MQ=60)
                                                    TATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTC                                           >  SRR4302543.7645084/1‑94 (MQ=60)
                                                    TATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTC                                           <  SRR4302543.930245/94‑1 (MQ=60)
                                                     ATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCA                                          >  SRR4302543.3958504/1‑94 (MQ=60)
                                                      TTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCAT                                         <  SRR4302543.6312493/94‑1 (MQ=60)
                                                      TTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCAT                                         >  SRR4302543.4537512/1‑94 (MQ=60)
                                                      TTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGCCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCAT                                         <  SRR4302543.4383819/94‑1 (MQ=60)
                                                       TGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATT                                        >  SRR4302543.523697/1‑94 (MQ=60)
                                                       TGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATT                                        <  SRR4302543.4022129/94‑1 (MQ=60)
                                                       TGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATT                                        >  SRR4302543.347437/1‑94 (MQ=60)
                                                        GTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTA                                       >  SRR4302543.5701971/1‑94 (MQ=60)
                                                         TCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAA                                      <  SRR4302543.7925820/94‑1 (MQ=60)
                                                           AGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAAC                                    <  SRR4302543.18249/94‑1 (MQ=60)
                                                           AGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAAC                                    <  SRR4302543.23836/94‑1 (MQ=60)
                                                           AGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGCCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAAC                                    <  SRR4302543.167649/94‑1 (MQ=60)
                                                            GTGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACA                                   <  SRR4302543.6995077/94‑1 (MQ=60)
                                                             TGAGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAA                                  <  SRR4302543.736485/94‑1 (MQ=60)
                                                               AGACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAAT                                >  SRR4302543.3963064/1‑94 (MQ=60)
                                                                 ACGAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCC                              <  SRR4302543.5085375/94‑1 (MQ=60)
                                                                   GAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCAT                            >  SRR4302543.5156771/1‑94 (MQ=60)
                                                                   GAAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCAT                            <  SRR4302543.934832/94‑1 (MQ=60)
                                                                    AAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATT                           <  SRR4302543.5509303/94‑1 (MQ=60)
                                                                    AAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATT                           <  SRR4302543.3184884/94‑1 (MQ=60)
                                                                    AAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATT                           <  SRR4302543.4620100/94‑1 (MQ=60)
                                                                    AAAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCCCCTTTCCCCGCTCATTCATTAAACAAATCCATT                           >  SRR4302543.7687191/1‑94 (MQ=60)
                                                                     AAATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTG                          <  SRR4302543.110965/94‑1 (MQ=60)
                                                                       ATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACCAATCCATTGCC                        >  SRR4302543.6144202/1‑94 (MQ=60)
                                                                       ATCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCC                        >  SRR4302543.5769526/1‑94 (MQ=60)
                                                                        TCCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCA                       >  SRR4302543.2196594/1‑94 (MQ=60)
                                                                         CCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCAT                      >  SRR4302543.6604609/1‑94 (MQ=60)
                                                                         CCAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCAT                      <  SRR4302543.6372829/94‑1 (MQ=60)
                                                                          CAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATA                     <  SRR4302543.964255/94‑1 (MQ=60)
                                                                          CAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATA                     >  SRR4302543.4597102/1‑94 (MQ=60)
                                                                          CAGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATA                     <  SRR4302543.5707955/94‑1 (MQ=60)
                                                                           AGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAA                    >  SRR4302543.446000/1‑94 (MQ=60)
                                                                           AGGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAA                    >  SRR4302543.446001/1‑94 (MQ=60)
                                                                            GGCTTCGCTCTTTTTGGCGCCCTCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAACCAAATCCATTGTCATAAA                   >  SRR4302543.6339450/1‑94 (MQ=60)
                                                                            GGCTTCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAA                   >  SRR4302543.6339449/1‑94 (MQ=60)
                                                                              CTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAAT                 >  SRR4302543.4215797/1‑94 (MQ=60)
                                                                                TCGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATAT               <  SRR4302543.2341772/94‑1 (MQ=60)
                                                                                 CGCTATTTTTGGCGCCCTCAGCTAAGAGGACAGTCCTCTTCGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTTCCATAAAATATA              >  SRR4302543.7129418/1‑94 (MQ=60)
                                                                                 CGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATA              >  SRR4302543.1112079/1‑94 (MQ=60)
                                                                                 CGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATA              >  SRR4302543.6073669/1‑94 (MQ=60)
                                                                                 CGCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATA              >  SRR4302543.7129417/1‑94 (MQ=60)
                                                                                  GCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATAT             <  SRR4302543.1428608/94‑1 (MQ=60)
                                                                                  GCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATAT             <  SRR4302543.4644246/94‑1 (MQ=60)
                                                                                  GCTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATAT             >  SRR4302543.6689301/1‑94 (MQ=60)
                                                                                   CTATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATA            <  SRR4302543.576112/94‑1 (MQ=60)
                                                                                     ATTTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAA          <  SRR4302543.2223948/94‑1 (MQ=60)
                                                                                       TTTTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAA        >  SRR4302543.4448025/1‑94 (MQ=60)
                                                                                        TGTGGCGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAA       <  SRR4302543.3459766/94‑1 (MQ=60)
                                                                                             CGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCT  >  SRR4302543.2948677/1‑94 (MQ=60)
                                                                                             CGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCT  <  SRR4302543.6095880/94‑1 (MQ=60)
                                                                                             CGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCT  <  SRR4302543.2490334/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTACAATGATGAGCGTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCT  >  NC_000913/2087764‑2087950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑