Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,080,323 T→C T85A (ACG→GCG)  dacD ← penicillin binding protein 6b

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,080,3230TC100.0% 854.6 / NA 271T85A (ACG→GCG) dacDpenicillin binding protein 6b
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (139/132);  total (139/132)

Only 100 of 280 total aligned reads displayed.

CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAC  >  NC_000913/2080233‑2080412
                                                                                          |                                                                                         
ccTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCTACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCaa                                                                                         <  2:3544206/93‑1 (MQ=255)
 ctctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAt                                                                                        >  2:7370085/1‑93 (MQ=255)
 ctctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAt                                                                                        >  1:1746353/1‑93 (MQ=255)
 ctctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAt                                                                                        <  2:5390035/93‑1 (MQ=255)
  tctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATg                                                                                       >  2:4093376/1‑93 (MQ=255)
  tctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATg                                                                                       >  1:4086159/1‑93 (MQ=255)
  tctTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATg                                                                                       >  1:4975849/1‑93 (MQ=255)
      tCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGAt                                                                                   <  2:7484714/93‑1 (MQ=255)
        aaaaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGa                                                                                 <  1:1814691/93‑1 (MQ=255)
          aaaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACt                                                                               >  1:7094931/1‑93 (MQ=255)
           aaCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTa                                                                              <  2:4977447/93‑1 (MQ=255)
            aCATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTAt                                                                             <  2:6881512/93‑1 (MQ=255)
             cATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATc                                                                            <  2:337683/93‑1 (MQ=255)
             cATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATc                                                                            >  2:6276841/1‑93 (MQ=255)
              aTCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCg                                                                           <  2:7108287/93‑1 (MQ=255)
              aTCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCg                                                                           >  1:2532738/1‑93 (MQ=255)
              aTCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCg                                                                           >  2:4128485/1‑93 (MQ=255)
               tCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGa                                                                          >  1:6743014/1‑93 (MQ=255)
                 aGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATa                                                                        <  2:5246037/93‑1 (MQ=255)
                 aGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATa                                                                        <  2:7635919/93‑1 (MQ=255)
                 aGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATa                                                                        <  1:3721325/93‑1 (MQ=255)
                  gTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAg                                                                       <  1:681823/93‑1 (MQ=255)
                   tGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAgc                                                                      <  2:1479935/93‑1 (MQ=255)
                   tGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAgc                                                                      >  1:2105102/1‑93 (MQ=255)
                       gaaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGa                                                                  >  2:303259/1‑93 (MQ=255)
                        aaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGAt                                                                 <  2:1497108/93‑1 (MQ=255)
                        aaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGAt                                                                 >  2:427847/1‑93 (MQ=255)
                        aaCCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGAt                                                                 >  2:7207076/1‑93 (MQ=255)
                           cGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCa                                                              <  1:3023393/93‑1 (MQ=255)
                            gACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCCCGAGCCAc                                                             >  2:3311855/1‑93 (MQ=255)
                            gACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGCTGACAATATCGTCTGTCGCAATGCGATGACTATCCATAGCGCGATCCAc                                                             >  1:6075146/1‑93 (MQ=255)
                              cAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGa                                                           <  1:3411927/93‑1 (MQ=255)
                              cAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGa                                                           >  2:2445534/1‑93 (MQ=255)
                              cAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGa                                                           <  1:7699835/93‑1 (MQ=255)
                              cAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGa                                                           >  1:1282813/1‑93 (MQ=255)
                                 acacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACAt                                                        <  1:565488/93‑1 (MQ=255)
                                  cacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGCCATa                                                       >  1:7416672/1‑93 (MQ=255)
                                  cacCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATa                                                       >  1:2465600/1‑93 (MQ=255)
                                   acCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATaa                                                      >  2:5670560/1‑93 (MQ=255)
                                   acCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATaa                                                      >  1:6576096/1‑93 (MQ=255)
                                    ccGGATTATCGTGTGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAc                                                     <  1:2720735/93‑1 (MQ=255)
                                     cGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAcc                                                    <  2:5658081/93‑1 (MQ=255)
                                     cGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAcc                                                    >  2:5076110/1‑93 (MQ=255)
                                      ggATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAccc                                                   <  1:2272861/93‑1 (MQ=255)
                                      ggATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAccc                                                   >  2:1808169/1‑93 (MQ=255)
                                      ggATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAccc                                                   <  1:2919855/93‑1 (MQ=255)
                                      ggATGATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAccc                                                   <  1:3123466/93‑1 (MQ=255)
                                      cgATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAccc                                                   <  2:2983491/92‑1 (MQ=255)
                                       gATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAATCCg                                                  >  1:4452152/1‑93 (MQ=255)
                                       gATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCg                                                  >  1:1057434/1‑93 (MQ=255)
                                          tATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtca                                               >  2:452076/1‑93 (MQ=255)
                                          tATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtca                                               >  2:677770/1‑93 (MQ=255)
                                            tCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtcatc                                             <  2:3720441/93‑1 (MQ=255)
                                             cTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGtcatca                                            >  2:7840243/1‑93 (MQ=255)
                                                tCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCt                                         <  1:909945/93‑1 (MQ=255)
                                                 cGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCtt                                        <  2:1971746/93‑1 (MQ=255)
                                                 cGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCtt                                        <  2:2310555/93‑1 (MQ=255)
                                                   cccACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTg                                      <  1:164304/93‑1 (MQ=255)
                                                    ccACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTgc                                     >  1:4518102/1‑92 (MQ=255)
                                                    ccACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGt                                     >  1:6390862/1‑93 (MQ=255)
                                                     cACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCCATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTc                                    >  2:4053829/1‑93 (MQ=255)
                                                     cACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTc                                    >  2:5624139/1‑93 (MQ=255)
                                                     cACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTc                                    >  2:4331367/1‑93 (MQ=255)
                                                      aCGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCa                                   >  1:718395/1‑93 (MQ=255)
                                                      aCGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCa                                   <  1:3917351/93‑1 (MQ=255)
                                                       cGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAg                                  <  1:811003/93‑1 (MQ=255)
                                                         cATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGc                                <  2:5136404/93‑1 (MQ=255)
                                                          aTCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCt                               <  1:6891746/93‑1 (MQ=255)
                                                           tCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTg                              >  1:539004/1‑93 (MQ=255)
                                                             gcgcCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGc                            <  2:3191734/93‑1 (MQ=255)
                                                                ccccACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCggg                         >  1:1317311/1‑93 (MQ=255)
                                                                 cccACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGa                        >  1:5976524/1‑93 (MQ=255)
                                                                  ccccGGTGACAATATCGTCTGGCGGAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGAt                       <  2:6531743/90‑1 (MQ=255)
                                                                  ccACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGAt                       >  2:1324922/1‑93 (MQ=255)
                                                                   cACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGAtt                      >  2:1159974/1‑93 (MQ=255)
                                                                   cACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGAtt                      <  1:2590101/93‑1 (MQ=255)
                                                                     cgttGACAATATCGTCTGGCGCAATGCGAGGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTgc                    <  2:4709620/90‑1 (MQ=255)
                                                                      ggTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGAGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGCCTGGCGGGGTTgac                   >  2:5736098/1‑91 (MQ=255)
                                                                       gTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTgcgc                  >  1:4257976/1‑93 (MQ=255)
                                                                       gTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTgcgc                  >  2:4621327/1‑93 (MQ=255)
                                                                        tGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCt                 >  2:2158079/1‑93 (MQ=255)
                                                                        tGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCt                 >  2:1092848/1‑93 (MQ=255)
                                                                         gACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTCGTCAGGCTGGCGGGATTGCGCTg                <  1:1717314/93‑1 (MQ=255)
                                                                          aCAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGt               <  1:4856424/93‑1 (MQ=255)
                                                                          aCAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGt               <  1:7204698/93‑1 (MQ=255)
                                                                             atatCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGa            <  1:1032809/93‑1 (MQ=255)
                                                                             atatCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGa            <  2:2664304/93‑1 (MQ=255)
                                                                              tatCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGAt           >  1:7558187/1‑93 (MQ=255)
                                                                               atCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGGTg          >  2:380313/1‑93 (MQ=255)
                                                                                 cGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCt        >  2:2841092/1‑93 (MQ=255)
                                                                                  gTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTc       >  2:1867498/1‑93 (MQ=255)
                                                                                  gTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTc       >  1:3818086/1‑93 (MQ=255)
                                                                                   tCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCa      <  1:856925/93‑1 (MQ=255)
                                                                                   tCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCa      >  2:87860/1‑93 (MQ=255)
                                                                                     tGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCAtt    <  2:6080349/93‑1 (MQ=255)
                                                                                     tGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCAtt    >  2:3674091/1‑93 (MQ=255)
                                                                                      ggCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCCCTGTTGATGCTCATTa   <  2:5217498/93‑1 (MQ=255)
                                                                                       gcgcAATGCTATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAc  <  1:6150428/93‑1 (MQ=255)
                                                                                       gcgcAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAc  <  2:6745101/93‑1 (MQ=255)
                                                                                       gcgcAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAc  <  1:840355/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTAC  >  NC_000913/2080233‑2080412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGC  >  NC_000913/2080230‑2080416
                                                                                             |                                                                                             
CGCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGC                                                                                               >  SRR4302543.4064663/1‑94 (MQ=60)
 GCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCA                                                                                              >  SRR4302543.2486932/1‑94 (MQ=60)
   CCTCTTTCAAAAACATCGGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAT                                                                                            <  SRR4302543.7115411/94‑1 (MQ=60)
   CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAT                                                                                            >  SRR4302543.7267737/1‑94 (MQ=60)
   CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAT                                                                                            >  SRR4302543.1761947/1‑94 (MQ=60)
   CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAT                                                                                            >  SRR4302543.1702339/1‑94 (MQ=60)
   CCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAAT                                                                                            <  SRR4302543.1186545/94‑1 (MQ=60)
    CTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATG                                                                                           >  SRR4302543.5021141/1‑94 (MQ=60)
    CTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATG                                                                                           >  SRR4302543.4122798/1‑94 (MQ=60)
    CTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATG                                                                                           >  SRR4302543.1787656/1‑94 (MQ=60)
     TCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGC                                                                                          >  SRR4302543.5926759/1‑94 (MQ=60)
     TCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGC                                                                                          <  SRR4302543.7736902/94‑1 (MQ=60)
     TCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGC                                                                                          <  SRR4302543.7280240/94‑1 (MQ=60)
      CTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCG                                                                                         >  SRR4302543.3921130/1‑94 (MQ=60)
      CTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCG                                                                                         <  SRR4302543.1321032/94‑1 (MQ=60)
      CTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCG                                                                                         >  SRR4302543.4644337/1‑94 (MQ=60)
        TTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGAT                                                                                       <  SRR4302543.635564/94‑1 (MQ=60)
         TCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATG                                                                                      <  SRR4302543.4949121/94‑1 (MQ=60)
           AAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGAC                                                                                    <  SRR4302543.1830969/94‑1 (MQ=60)
           AAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGAC                                                                                    <  SRR4302543.7493502/94‑1 (MQ=60)
            AAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACT                                                                                   >  SRR4302543.7163828/1‑94 (MQ=60)
            AAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACT                                                                                   >  SRR4302543.7163829/1‑94 (MQ=60)
             AAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTA                                                                                  >  SRR4302543.1305141/1‑94 (MQ=60)
              AACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTAT                                                                                 <  SRR4302543.3706719/94‑1 (MQ=60)
               ACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATC                                                                                <  SRR4302543.3766945/94‑1 (MQ=60)
               ACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATC                                                                                >  SRR4302543.7798991/1‑94 (MQ=60)
               ACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATC                                                                                <  SRR4302543.6688333/94‑1 (MQ=60)
                CATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCG                                                                               >  SRR4302543.2555799/1‑94 (MQ=60)
                 ATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGA                                                                              >  SRR4302543.6806692/1‑94 (MQ=60)
                 ATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGA                                                                              >  SRR4302543.4295163/1‑94 (MQ=60)
                 ATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGA                                                                              <  SRR4302543.1989624/94‑1 (MQ=60)
                  TCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGAT                                                                             <  SRR4302543.6078377/94‑1 (MQ=60)
                   CAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATA                                                                            <  SRR4302543.876580/94‑1 (MQ=60)
                    AGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAG                                                                           >  SRR4302543.6462092/1‑94 (MQ=60)
                    AGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAG                                                                           <  SRR4302543.3754053/94‑1 (MQ=60)
                     GTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGC                                                                          >  SRR4302543.2123937/1‑94 (MQ=60)
                     GTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGC                                                                          <  SRR4302543.688119/94‑1 (MQ=60)
                     GTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGC                                                                          >  SRR4302543.2699646/1‑94 (MQ=60)
                     GTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATAGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGC                                                                          >  SRR4302543.7714194/1‑94 (MQ=60)
                          GAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGAT                                                                     <  SRR4302543.6364604/94‑1 (MQ=60)
                           AACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATC                                                                    >  SRR4302543.2529511/1‑94 (MQ=60)
                           AACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATC                                                                    <  SRR4302543.3703894/94‑1 (MQ=60)
                              CGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCAC                                                                 <  SRR4302543.3050721/94‑1 (MQ=60)
                              CGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGCTGACAATATCGTCTGTCGCAATGCGATGACTATCCATAGCGCGATCCAC                                                                 >  SRR4302543.6132725/1‑94 (MQ=60)
                               GACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACG                                                                >  SRR4302543.3921150/1‑94 (MQ=60)
                                ACAAACACCGGATTATCTTTCGCCCACGCATCGTGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGA                                                               >  SRR4302543.5298839/1‑94 (MQ=60)
                                ACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGTCTATCGATAGCGCGATCAACGA                                                               >  SRR4302543.4813235/1‑94 (MQ=60)
                                ACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGA                                                               >  SRR4302543.1294252/1‑94 (MQ=60)
                                 CAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGAC                                                              <  SRR4302543.7772713/94‑1 (MQ=60)
                                 CAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGAC                                                              <  SRR4302543.3442257/94‑1 (MQ=60)
                                 CAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGAC                                                              <  SRR4302543.3442471/94‑1 (MQ=60)
                                   AACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACAT                                                            <  SRR4302543.3765080/94‑1 (MQ=60)
                                    ACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGCCATA                                                           >  SRR4302543.7487820/1‑94 (MQ=60)
                                    ACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATA                                                           <  SRR4302543.570668/94‑1 (MQ=60)
                                    ACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATA                                                           >  SRR4302543.2487845/1‑94 (MQ=60)
                                     CACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAC                                                          >  SRR4302543.2712564/1‑94 (MQ=60)
                                     CACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAA                                                          >  SRR4302543.6638018/1‑94 (MQ=60)
                                     CACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAA                                                          >  SRR4302543.564405/1‑94 (MQ=60)
                                      ACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAAC                                                         <  SRR4302543.5440364/94‑1 (MQ=60)
                                       CCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACC                                                        <  SRR4302543.2745475/94‑1 (MQ=60)
                                       CCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACC                                                        <  SRR4302543.228389/94‑1 (MQ=60)
                                       CCGGATTATCGTGTGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACC                                                        <  SRR4302543.2745474/94‑1 (MQ=60)
                                        CGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCC                                                       >  SRR4302543.6833918/1‑94 (MQ=60)
                                        CGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCC                                                       >  SRR4302543.4021844/1‑94 (MQ=60)
                                        CGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCC                                                       >  SRR4302543.1560631/1‑94 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAATCCG                                                      >  SRR4302543.4492469/1‑94 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      >  SRR4302543.822408/1‑94 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      >  SRR4302543.1066919/1‑94 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      >  SRR4302543.1385403/1‑94 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      <  SRR4302543.2293637/94‑1 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      >  SRR4302543.1066918/1‑94 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      >  SRR4302543.2556647/1‑94 (MQ=60)
                                         GGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      <  SRR4302543.2946388/94‑1 (MQ=60)
                                         GGATGATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCG                                                      <  SRR4302543.3151559/94‑1 (MQ=60)
                                          GATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGT                                                     >  SRR4302543.7138296/1‑94 (MQ=60)
                                           ATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTC                                                    >  SRR4302543.965128/1‑94 (MQ=60)
                                           ATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTC                                                    >  SRR4302543.1799364/1‑94 (MQ=60)
                                            TTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCA                                                   >  SRR4302543.2772622/1‑94 (MQ=60)
                                            TTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCA                                                   >  SRR4302543.5029568/1‑94 (MQ=60)
                                            TTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCA                                                   <  SRR4302543.6871560/94‑1 (MQ=60)
                                            TTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCA                                                   >  SRR4302543.2604121/1‑94 (MQ=60)
                                             TATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCAT                                                  >  SRR4302543.7265425/1‑94 (MQ=60)
                                               TCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCA                                                <  SRR4302543.4504034/94‑1 (MQ=60)
                                                CGTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAG                                               <  SRR4302543.6899486/94‑1 (MQ=60)
                                                 TTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGC                                              <  SRR4302543.3102458/94‑1 (MQ=60)
                                                  TTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCT                                             <  SRR4302543.2454016/94‑1 (MQ=60)
                                                   TCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTT                                            <  SRR4302543.917943/94‑1 (MQ=60)
                                                     GCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTG                                          >  SRR4302543.1744480/1‑94 (MQ=60)
                                                      CCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGT                                         >  SRR4302543.3014044/1‑94 (MQ=60)
                                                      CCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGT                                         <  SRR4302543.165720/94‑1 (MQ=60)
                                                      CCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGT                                         >  SRR4302543.6451019/1‑94 (MQ=60)
                                                      CCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGC                                         >  SRR4302543.4559197/1‑94 (MQ=60)
                                                       CCACGCCTCCCGCCCCGCGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAGCCCGTCATCAGCTTTGTC                                        <  SRR4302543.2245925/94‑1 (MQ=60)
                                                       CCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTC                                        >  SRR4302543.4181052/1‑94 (MQ=60)
                                                       CCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTC                                        >  SRR4302543.435991/1‑94 (MQ=60)
                                                        CACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCA                                       >  SRR4302543.724889/1‑94 (MQ=60)
                                                        CACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCA                                       <  SRR4302543.4299062/94‑1 (MQ=60)
                                                         ACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAG                                      <  SRR4302543.3951924/94‑1 (MQ=60)
                                                         ACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAG                                      <  SRR4302543.1292939/94‑1 (MQ=60)
                                                          CGCATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGG                                     <  SRR4302543.818146/94‑1 (MQ=60)
                                                            CATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATATCGCGATCCACCACATAACCCGTCATCAGCTTTGTCAGGCT                                   >  SRR4302543.669510/1‑94 (MQ=60)
                                                             ATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTG                                  <  SRR4302543.7184746/94‑1 (MQ=60)
                                                             ATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTG                                  >  SRR4302543.829491/1‑94 (MQ=60)
                                                             ATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTG                                  <  SRR4302543.6956542/94‑1 (MQ=60)
                                                             ATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTG                                  >  SRR4302543.543998/1‑94 (MQ=60)
                                                             ATCGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTG                                  >  SRR4302543.6056885/1‑94 (MQ=60)
                                                               CGCGCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGC                                <  SRR4302543.1673755/94‑1 (MQ=60)
                                                                  GCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGG                             <  SRR4302543.3034930/94‑1 (MQ=60)
                                                                  GCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGG                             >  SRR4302543.1329018/1‑94 (MQ=60)
                                                                  GCCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGG                             <  SRR4302543.5876395/94‑1 (MQ=60)
                                                                   CCCCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGA                            >  SRR4302543.6031271/1‑94 (MQ=60)
                                                                     CCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATT                          <  SRR4302543.2361036/94‑1 (MQ=60)
                                                                     CCACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATT                          <  SRR4302543.1878322/94‑1 (MQ=60)
                                                                      CACGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTG                         <  SRR4302543.2613645/94‑1 (MQ=60)
                                                                        CGGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCG                       >  SRR4302543.3329536/1‑94 (MQ=60)
                                                                         GGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGC                      >  SRR4302543.4296638/1‑94 (MQ=60)
                                                                         GGTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGC                      <  SRR4302543.6521388/94‑1 (MQ=60)
                                                                          GTGACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCT                     <  SRR4302543.6172499/94‑1 (MQ=60)
                                                                            GACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTCGTCAGGCTGGCGGGATTGCGCTGT                   <  SRR4302543.1732654/94‑1 (MQ=60)
                                                                             ACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTT                  <  SRR4302543.7274549/94‑1 (MQ=60)
                                                                             ACAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTT                  <  SRR4302543.4900706/94‑1 (MQ=60)
                                                                              CAATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTG                 >  SRR4302543.7907249/1‑94 (MQ=60)
                                                                               AATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGA                <  SRR4302543.7428051/94‑1 (MQ=60)
                                                                                ATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGAT               <  SRR4302543.1041980/94‑1 (MQ=60)
                                                                                ATATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGAT               >  SRR4302543.7630146/1‑94 (MQ=60)
                                                                                 TATCGTCTGGCGCAGTGCGATGACTATCGATAGCGCGATGCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATG              <  SRR4302543.4555879/94‑1 (MQ=60)
                                                                                 TATCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATG              <  SRR4302543.858269/94‑1 (MQ=60)
                                                                                 TATCGTCTGGCCCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATG              <  SRR4302543.858270/94‑1 (MQ=60)
                                                                                   TCGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCT            <  SRR4302543.4531775/94‑1 (MQ=60)
                                                                                    CGTCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTC           >  SRR4302543.3851749/1‑94 (MQ=60)
                                                                                      TCTGGCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCAT         <  SRR4302543.864556/94‑1 (MQ=60)
                                                                                          GCGCAATGCTATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACC     <  SRR4302543.6208664/94‑1 (MQ=60)
                                                                                          GCGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACC     <  SRR4302543.847773/94‑1 (MQ=60)
                                                                                           CGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGGTTGCGCTGTTGATGGTCATTACCC    >  SRR4302543.5023549/1‑94 (MQ=60)
                                                                                           CGCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCC    <  SRR4302543.159669/94‑1 (MQ=60)
                                                                                            GCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCG   <  SRR4302543.6993380/94‑1 (MQ=60)
                                                                                            GCAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCG   <  SRR4302543.7948406/94‑1 (MQ=60)
                                                                                             CAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCGTCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGC  >  SRR4302543.1290444/1‑94 (MQ=60)
                                                                                             CAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGC  >  SRR4302543.5619954/1‑94 (MQ=60)
                                                                                             CAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGC  >  SRR4302543.1406427/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCCCTCTTTCAAAAACATCAGTGAAGAACCGACAAACACCGGATTATCTTTCGCCCACGCATCGCGCCCCACGGTGACAATATCGTCTGGCGTAATGCGATGACTATCGATAGCGCGATCCACGACATAACCCGTCATCAGCTTTGTCAGGCTGGCGGGATTGCGCTGTTGATGCTCATTACCCGC  >  NC_000913/2080230‑2080416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑