Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,092,192 A→G T235A (ACC→GCC)  b2022 → imidazoleglycerolphosphate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,092,1920AG100.0% 382.3 / NA 120T235A (ACC→GCC) b2022imidazoleglycerolphosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (62/58);  total (62/58)

Only 100 of 124 total aligned reads displayed.

CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAA  >  NC_000913/2092102‑2092281
                                                                                          |                                                                                         
cTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGcc                                                                                         <  2:1897868/93‑1 (MQ=255)
 tGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCg                                                                                        <  2:94573/93‑1 (MQ=255)
 tGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCg                                                                                        >  1:4283788/1‑93 (MQ=255)
  ggATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCgg                                                                                       <  1:2252391/93‑1 (MQ=255)
    aTCAGATCTCTGCCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGcc                                                                                     <  2:4265379/93‑1 (MQ=255)
    aTCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGcc                                                                                     <  2:3689519/93‑1 (MQ=255)
     tCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCt                                                                                    >  1:3644822/1‑93 (MQ=255)
      cAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTg                                                                                   <  1:1361908/93‑1 (MQ=255)
      cAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTg                                                                                   <  1:941607/93‑1 (MQ=255)
       aGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTgg                                                                                  >  1:3039331/1‑93 (MQ=255)
       aGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTgg                                                                                  >  1:3013427/1‑93 (MQ=255)
           cGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCt                                                                              >  2:4394286/1‑93 (MQ=255)
            gCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTg                                                                             >  2:4553989/1‑93 (MQ=255)
            gCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTg                                                                             <  1:3951026/93‑1 (MQ=255)
             cTACCCAAGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCCCCACACCGTCGAAGATGCCGGCCTGGCGCTgg                                                                            <  1:500975/93‑1 (MQ=255)
              tACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTggg                                                                           >  1:2196374/1‑93 (MQ=255)
              tACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTggg                                                                           >  2:4063508/1‑93 (MQ=255)
               aCCCACGACGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGc                                                                          <  2:418918/93‑1 (MQ=255)
                cccACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCg                                                                         >  1:1086864/1‑93 (MQ=255)
                 ccACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGa                                                                        >  2:3562279/1‑93 (MQ=255)
                  cACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGaa                                                                       <  2:1477628/93‑1 (MQ=255)
                   aCGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAg                                                                      <  2:3443661/93‑1 (MQ=255)
                   aCGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAg                                                                      >  1:1901343/1‑93 (MQ=255)
                    cggcggTTTCCGCATGGAAATCCACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAgc                                                                     <  1:1663593/93‑1 (MQ=255)
                    cggcggTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAgc                                                                     <  1:196365/93‑1 (MQ=255)
                        ggTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTa                                                                 >  2:2600864/1‑93 (MQ=255)
                          tttCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTaaa                                                               >  1:1091233/1‑93 (MQ=255)
                            tCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTaaaaa                                                             <  1:175040/93‑1 (MQ=255)
                               gCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCg                                                          >  1:2268669/1‑93 (MQ=255)
                               gCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCg                                                          >  1:616789/1‑93 (MQ=255)
                                cATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGc                                                         >  1:129284/1‑93 (MQ=255)
                                cATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTAGCGCTGGGCGAAGCGCTAAAAATCGc                                                         >  2:1624819/1‑93 (MQ=255)
                                 aTGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGcc                                                        >  1:4146846/1‑93 (MQ=255)
                                     aaaTCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCg                                                    <  1:3056156/93‑1 (MQ=255)
                                      aaTCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGACCTCgg                                                   >  2:4279399/1‑93 (MQ=255)
                                        tCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGAGCTGGGAGAAGCGCTAAAAATCGCCCTCGgag                                                 >  2:3120349/1‑93 (MQ=255)
                                         cAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGgaga                                                <  1:3258940/93‑1 (MQ=255)
                                         cAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGAGCTGGGCGAAGCGCTAAAAATCGCCCTCGgaga                                                >  1:2850286/1‑93 (MQ=255)
                                          aaCGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAc                                               <  1:2637124/93‑1 (MQ=255)
                                          aaCGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAc                                               <  1:2547238/93‑1 (MQ=255)
                                           aCGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGAGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACa                                              >  2:3656763/1‑93 (MQ=255)
                                             gTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACaaa                                            <  2:1920995/93‑1 (MQ=255)
                                             gTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACaaa                                            >  2:3086450/1‑93 (MQ=255)
                                             gTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGAGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACaaa                                            >  1:3013444/1‑93 (MQ=255)
                                              tCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAc                                           >  2:3333273/1‑93 (MQ=255)
                                                aaaGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAcgc                                         <  1:3411816/93‑1 (MQ=255)
                                                aaaGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAcgc                                         <  1:4278438/93‑1 (MQ=255)
                                                 aaGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCCATAAATCGCCCTCGGAGACAAAcgtg                                        >  2:1941515/1‑91 (MQ=255)
                                                  aGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCgg                                       >  2:4564972/1‑93 (MQ=255)
                                                    gCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTa                                     <  2:695466/93‑1 (MQ=255)
                                                    gCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTa                                     >  2:1207978/1‑93 (MQ=255)
                                                     cGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAt                                    <  2:1170825/93‑1 (MQ=255)
                                                      gACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGATACAAACGCGGTAtt                                   >  1:1749150/1‑93 (MQ=255)
                                                      gACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAtt                                   <  1:2551093/93‑1 (MQ=255)
                                                      gACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAtt                                   >  2:75180/1‑93 (MQ=255)
                                                       aCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAttt                                  >  2:1353981/1‑93 (MQ=255)
                                                        ccTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTg                                 <  2:3889830/93‑1 (MQ=255)
                                                        ccTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTg                                 <  2:4020653/93‑1 (MQ=255)
                                                         ctctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGc                                >  2:4322929/1‑93 (MQ=255)
                                                          tctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGcc                               >  1:4514552/1‑93 (MQ=255)
                                                          tctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGcc                               >  1:3378412/1‑93 (MQ=255)
                                                            tataTCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGc                             <  2:2287577/93‑1 (MQ=255)
                                                            tataTCGACGATCACCACACCGTCGAAGATGCACGCCTGGCGCCTGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGc                             >  1:980088/1‑93 (MQ=255)
                                                             atatCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCt                            >  1:3885358/1‑93 (MQ=255)
                                                             atatCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCt                            <  2:4118528/93‑1 (MQ=255)
                                                             atatCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCt                            >  2:1044582/1‑93 (MQ=255)
                                                             atatCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCACTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCt                            <  2:114427/93‑1 (MQ=255)
                                                               atCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCttt                          <  1:1906227/93‑1 (MQ=255)
                                                                tCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTg                         <  2:645137/93‑1 (MQ=255)
                                                                tCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTg                         <  2:2843723/93‑1 (MQ=255)
                                                                tCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTg                         <  1:4471240/93‑1 (MQ=255)
                                                                  gacgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGt                       <  2:2846346/93‑1 (MQ=255)
                                                                   acgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGtt                      >  1:3898312/1‑93 (MQ=255)
                                                                   acgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGtt                      <  1:3685918/93‑1 (MQ=255)
                                                                   acgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGtt                      >  2:3281110/1‑93 (MQ=255)
                                                                      aTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTtg                   >  1:1299647/1‑93 (MQ=255)
                                                                      aTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTtg                   <  2:188384/93‑1 (MQ=255)
                                                                       tCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTtgt                  >  1:502600/1‑93 (MQ=255)
                                                                         accacACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGc                >  2:459859/1‑93 (MQ=255)
                                                                          ccacACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCt               <  1:2522012/93‑1 (MQ=255)
                                                                          ccacACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCt               >  2:360059/1‑93 (MQ=255)
                                                                           cacaCCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTa              >  2:1638613/1‑93 (MQ=255)
                                                                           cacaCCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTa              >  2:1076924/1‑93 (MQ=255)
                                                                            ccacCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAc             <  1:1899845/92‑1 (MQ=255)
                                                                            acacCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAc             >  2:203882/1‑93 (MQ=255)
                                                                            acacCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAc             >  2:3708771/1‑93 (MQ=255)
                                                                            acacCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAc             <  1:4479867/93‑1 (MQ=255)
                                                                             cacCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAcc            <  1:868936/93‑1 (MQ=255)
                                                                               ccGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGa          >  1:3121938/1‑93 (MQ=255)
                                                                                cGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGAt         <  1:3973396/93‑1 (MQ=255)
                                                                                  tCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATgg       >  1:516114/1‑93 (MQ=255)
                                                                                  tCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATgg       >  1:148324/1‑93 (MQ=255)
                                                                                  tCGAAGATGCCGGCCCGGCGCTGGGGGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGGATTTGCCGCCTTTGGTTTGTGCTTCCGacag       >  2:164818/1‑90 (MQ=255)
                                                                                    gAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGAc     <  1:4552996/93‑1 (MQ=255)
                                                                                    gAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGAc     >  1:4436308/1‑93 (MQ=255)
                                                                                    gAAGATGCCGGCCTGGCACTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGat     >  1:3040902/1‑92 (MQ=255)
                                                                                      aGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGa   >  2:384283/1‑93 (MQ=255)
                                                                                       gATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  <  1:2534837/93‑1 (MQ=255)
                                                                                       gATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  >  2:2521957/1‑93 (MQ=255)
                                                                                       gATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  <  1:2216925/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAA  >  NC_000913/2092102‑2092281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATG  >  NC_000913/2092099‑2092283
                                                                                             |                                                                                           
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATG                                                                                             <  SRR4302535.1067185/94‑1 (MQ=60)
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATG                                                                                             <  SRR4302535.2230066/94‑1 (MQ=60)
 TGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGC                                                                                            <  SRR4302535.4623994/94‑1 (MQ=60)
 TGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGC                                                                                            >  SRR4302535.1695842/1‑94 (MQ=60)
 TGCTGGAACAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGC                                                                                            <  SRR4302535.971661/94‑1 (MQ=60)
  GCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCC                                                                                           <  SRR4302535.2309875/94‑1 (MQ=60)
  GCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCC                                                                                           <  SRR4302535.260652/94‑1 (MQ=60)
  GCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCC                                                                                           <  SRR4302535.4488528/94‑1 (MQ=60)
   CTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCG                                                                                          >  SRR4302535.4313094/1‑94 (MQ=60)
    TGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGG                                                                                         <  SRR4302535.3324328/94‑1 (MQ=60)
    TGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGG                                                                                         >  SRR4302535.4469182/1‑94 (MQ=60)
     GGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGC                                                                                        <  SRR4302535.2268429/94‑1 (MQ=60)
       ATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCT                                                                                      <  SRR4302535.4100812/94‑1 (MQ=60)
       ATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCT                                                                                      >  SRR4302535.3670065/1‑94 (MQ=60)
        TCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTG                                                                                     <  SRR4302535.692413/94‑1 (MQ=60)
         CAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGG                                                                                    >  SRR4302535.3060618/1‑94 (MQ=60)
         CAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGG                                                                                    >  SRR4302535.3034544/1‑94 (MQ=60)
         CAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGG                                                                                    <  SRR4302535.1371648/94‑1 (MQ=60)
         CAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGG                                                                                    <  SRR4302535.948272/94‑1 (MQ=60)
           GATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCG                                                                                  >  SRR4302535.2571356/1‑94 (MQ=60)
               GCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGG                                                                              <  SRR4302535.3978003/94‑1 (MQ=60)
                CTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGG                                                                             >  SRR4302535.2212030/1‑94 (MQ=60)
                CTACCCAAGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCCCCACACCGTCGAAGATGCCGGCCTGGCGCTGGG                                                                             <  SRR4302535.504482/94‑1 (MQ=60)
                  ACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCG                                                                           >  SRR4302535.1094590/1‑94 (MQ=60)
                     CACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAG                                                                        >  SRR4302535.1915027/1‑94 (MQ=60)
                       CGGCGGTTTCCGCATGGAAATCCACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCG                                                                      <  SRR4302535.1675613/94‑1 (MQ=60)
                       CGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCG                                                                      <  SRR4302535.197716/94‑1 (MQ=60)
                            GTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAA                                                                 >  SRR4302535.1099009/1‑94 (MQ=60)
                               TCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAAT                                                              <  SRR4302535.176254/94‑1 (MQ=60)
                               TCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAAT                                                              <  SRR4302535.1372701/94‑1 (MQ=60)
                                 CGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCG                                                            >  SRR4302535.621080/1‑94 (MQ=60)
                                 CGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCG                                                            >  SRR4302535.2284843/1‑94 (MQ=60)
                                  GCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGC                                                           >  SRR4302535.130193/1‑94 (MQ=60)
                                   CATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCC                                                          >  SRR4302535.4175242/1‑94 (MQ=60)
                                        AAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGG                                                     <  SRR4302535.3077546/94‑1 (MQ=60)
                                          ATCAACGTCAAAGGCGACCTCTATATCGACGATCACCAAACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCACTCGGAG                                                   >  SRR4302535.1544741/1‑94 (MQ=60)
                                           TCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGAGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGA                                                  >  SRR4302535.2870483/1‑94 (MQ=60)
                                            CAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAC                                                 <  SRR4302535.3281591/94‑1 (MQ=60)
                                            CAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAC                                                 <  SRR4302535.303881/94‑1 (MQ=60)
                                             AACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACA                                                <  SRR4302535.2656016/94‑1 (MQ=60)
                                             AACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACA                                                <  SRR4302535.2565567/94‑1 (MQ=60)
                                               CGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGAGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAA                                              >  SRR4302535.3034561/1‑94 (MQ=60)
                                                   AAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCG                                          <  SRR4302535.3435555/94‑1 (MQ=60)
                                                   AAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCG                                          <  SRR4302535.4307718/94‑1 (MQ=60)
                                                        CGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGATACAAACGCGGTATT                                     >  SRR4302535.1761802/1‑94 (MQ=60)
                                                         GACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                    <  SRR4302535.2569462/94‑1 (MQ=60)
                                                            CTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCC                                 >  SRR4302535.4545508/1‑94 (MQ=60)
                                                            CTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCC                                 >  SRR4302535.3401901/1‑94 (MQ=60)
                                                              CTATATCGACGATCACCACACCGTCGAAGATGCACGCCTGGCGCCTGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGC                               >  SRR4302535.987054/1‑94 (MQ=60)
                                                               TATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCT                              >  SRR4302535.3912009/1‑94 (MQ=60)
                                                                  ATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTG                           <  SRR4302535.1919945/94‑1 (MQ=60)
                                                                   TCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGG                          <  SRR4302535.4501922/94‑1 (MQ=60)
                                                                     GACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTT                        >  SRR4302535.3925019/1‑94 (MQ=60)
                                                                      ACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTT                       <  SRR4302535.3711418/94‑1 (MQ=60)
                                                                        GATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTG                     >  SRR4302535.1308912/1‑94 (MQ=60)
                                                                         ATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGT                    >  SRR4302535.506114/1‑94 (MQ=60)
                                                                             CCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTA                <  SRR4302535.1770883/94‑1 (MQ=60)
                                                                             CCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTA                <  SRR4302535.2540187/94‑1 (MQ=60)
                                                                               CCACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACC              <  SRR4302535.1913517/94‑1 (MQ=60)
                                                                               ACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACC              <  SRR4302535.4510620/94‑1 (MQ=60)
                                                                               ACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACC              >  SRR4302535.4641574/1‑94 (MQ=60)
                                                                                CACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCG             <  SRR4302535.875087/94‑1 (MQ=60)
                                                                                 ACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGA            >  SRR4302535.3143680/1‑94 (MQ=60)
                                                                                   CGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATG          <  SRR4302535.4000537/94‑1 (MQ=60)
                                                                                    GTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGG         >  SRR4302535.149357/1‑94 (MQ=60)
                                                                                    GTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGG         >  SRR4302535.519722/1‑94 (MQ=60)
                                                                                    GTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGG         >  SRR4302535.2072471/1‑94 (MQ=60)
                                                                                     TCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGA        <  SRR4302535.2482661/94‑1 (MQ=60)
                                                                                      CGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGAC       >  SRR4302535.4466712/1‑94 (MQ=60)
                                                                                      CGAAGATGCCGGCCTGGCACTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGAT       >  SRR4302535.3062204/1‑94 (MQ=60)
                                                                                       GAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACG      <  SRR4302535.4584106/94‑1 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT   <  SRR4302535.2553096/94‑1 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT   <  SRR4302535.3798389/94‑1 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT   <  SRR4302535.2232706/94‑1 (MQ=60)
                                                                                           ATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATC  >  SRR4302535.601603/1‑94 (MQ=60)
                                                                                           ATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGCTTTGTGCTACCGATGGACGAATC  >  SRR4302535.2070708/1‑94 (MQ=60)
                                                                                             |                                                                                           
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATG  >  NC_000913/2092099‑2092283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑