Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,147,364	A→G	R548R (CGT→CGC)	b2070 ←	putative chaperonin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,147,364	0	A	G	100.0%	370.0 / NA	117	R548R (CGT→CGC)	b2070	putative chaperonin
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (66/51); total (66/51)

Only 100 of 136 total aligned reads displayed.

CCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATC  >  NC_000913/2147274‑2147453

ccTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCcgcg                                                                                         <  2:764781/93‑1 (MQ=255)

ccTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCcgcg                                                                                         <  1:3530821/93‑1 (MQ=255)

ccTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCcgcg                                                                                         >  2:2003380/1‑93 (MQ=255)

 cTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCgcga                                                                                        <  1:1455853/93‑1 (MQ=255)

 cTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCgcga                                                                                        <  1:997407/93‑1 (MQ=255)

  tGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCgcgac                                                                                       >  2:4259544/1‑93 (MQ=255)

  tGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCgcgac                                                                                       >  2:3483086/1‑93 (MQ=255)

  tGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCgcgac                                                                                       >  1:3667834/1‑93 (MQ=255)

  tGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCgcgac                                                                                       >  1:1943943/1‑93 (MQ=255)

   gAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCgcgacg                                                                                      <  1:1856493/93‑1 (MQ=255)

      cacaAACCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGt                                                                                   <  2:3471036/93‑1 (MQ=255)

      cacaAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGt                                                                                   >  2:718273/1‑93 (MQ=255)

       acaAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTa                                                                                  <  2:3978178/93‑1 (MQ=255)

       acaAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTa                                                                                  >  1:3213134/1‑93 (MQ=255)

          aaaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACa                                                                               <  2:4532580/93‑1 (MQ=255)

          aaaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACa                                                                               >  1:731055/1‑93 (MQ=255)

          aaaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACa                                                                               <  1:483072/93‑1 (MQ=255)

          aaaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACa                                                                               <  1:1059370/93‑1 (MQ=255)

           aaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACAt                                                                              >  1:1114587/1‑93 (MQ=255)

           aaCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACAt                                                                              >  1:691559/1‑93 (MQ=255)

            aCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATc                                                                             <  1:3124282/93‑1 (MQ=255)

            aCGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATc                                                                             <  1:119308/93‑1 (MQ=255)

             cGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCt                                                                            >  2:1271679/1‑93 (MQ=255)

              ggCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGGTGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTg                                                                           >  1:785931/1‑93 (MQ=255)

               gCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGc                                                                          >  1:3950324/1‑93 (MQ=255)

               gCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGc                                                                          >  1:3674959/1‑93 (MQ=255)

               gCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGc                                                                          <  1:1593769/93‑1 (MQ=255)

                  ttCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGc                                                                       <  2:1917220/93‑1 (MQ=255)

                  ttCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGc                                                                       <  1:3695520/93‑1 (MQ=255)

                  ttCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGc                                                                       >  1:1643980/1‑93 (MQ=255)

                    cacaGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTg                                                                     <  1:2735165/93‑1 (MQ=255)

                    cacaGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTg                                                                     <  1:3432021/93‑1 (MQ=255)

                        gCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTc                                                                 <  2:3239216/93‑1 (MQ=255)

                          tCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCaa                                                               >  2:1015165/1‑93 (MQ=255)

                          tCGCTTTCCAATCGATAACCTCCTCGGTCAGATGGTCGCCAAACCCCTTCACTTGCTTAACGCCGCGAAGGGCAACATCTGCGGCTGTTTTaa                                                               >  2:2848229/1‑93 (MQ=255)

                           cGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAAt                                                              >  1:223455/1‑93 (MQ=255)

                            gCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATa                                                             >  1:1470165/1‑93 (MQ=255)

                              tttCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATAcc                                                           >  1:3129083/1‑93 (MQ=255)

                                tCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCaa                                                         <  2:4678524/93‑1 (MQ=255)

                                 ccAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCaaa                                                        <  2:2576916/93‑1 (MQ=255)

                                 ccAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCaaa                                                        >  1:2325945/1‑93 (MQ=255)

                                  cAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCaaaa                                                       <  1:393848/93‑1 (MQ=255)

                                  cAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCaaaa                                                       <  2:2170488/93‑1 (MQ=255)

                                  cAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCaaaa                                                       <  1:2436005/93‑1 (MQ=255)

                                   aGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAg                                                      <  2:2191270/93‑1 (MQ=255)

                                    gTCGATAACCGCCTGGGTCAGATGGTCGCCGAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGa                                                     >  2:2862086/1‑93 (MQ=255)

                                      cGATAACCGCCTGGGTCAGATGGTCGCCAAACTCTTGCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATCCCAAAAGAcc                                                   <  2:3207616/93‑1 (MQ=255)

                                        aTAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGt                                                 >  2:3149809/1‑93 (MQ=255)

                                        aTAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGt                                                 <  2:144007/93‑1 (MQ=255)

                                         tAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTa                                                >  1:1231063/1‑93 (MQ=255)

                                         tAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTa                                                >  2:4222801/1‑93 (MQ=255)

                                         tAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTa                                                >  2:1110451/1‑93 (MQ=255)

                                           aCCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAAc                                              >  2:3898430/1‑93 (MQ=255)

                                            ccGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAAcg                                             <  2:1418338/93‑1 (MQ=255)

                                             cGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAAcgc                                            <  1:4658963/93‑1 (MQ=255)

                                             cGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAAcgc                                            >  2:2514403/1‑93 (MQ=255)

                                             cGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAAcgc                                            <  1:1146269/93‑1 (MQ=255)

                                              gCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAAcgcc                                           >  2:4255836/1‑93 (MQ=255)

                                                cTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGATCGTAAcgccga                                         >  1:2174138/1‑92 (MQ=255)

                                                cTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGCAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAAcgccgc                                         >  2:1996675/1‑93 (MQ=255)

                                                 tGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCt                                        >  2:4623828/1‑93 (MQ=255)

                                                  gggTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCtt                                       <  2:1855080/93‑1 (MQ=255)

                                                   ggTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCttt                                      >  1:1928291/1‑93 (MQ=255)

                                                     tCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTAc                                    >  1:3525358/1‑93 (MQ=255)

                                                       aGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTAcgc                                  >  1:1030624/1‑93 (MQ=255)

                                                       aGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTAcgc                                  <  1:2981474/93‑1 (MQ=255)

                                                        gATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTAcgcg                                 >  2:3167830/1‑93 (MQ=255)

                                                            gTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAgg                             >  2:1660059/1‑93 (MQ=255)

                                                             tCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAggg                            >  2:550138/1‑93 (MQ=255)

                                                              cGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGc                           <  2:1664231/93‑1 (MQ=255)

                                                               gCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGcc                          >  2:4507878/1‑93 (MQ=255)

                                                               gCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGcc                          >  1:1746921/1‑93 (MQ=255)

                                                                ccAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCa                         >  1:1920944/1‑93 (MQ=255)

                                                                 cAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACACCGCTTTACGCGCAGGGACaa                        >  1:1442834/1‑93 (MQ=255)

                                                                  aaaCCCTTTCACTTGCTTTAAGCCGCGGCGGGGAAAATCTGCGGGTGGTTCAATTCCCAAAGGCCGTAAAGCCGCCTTACGCGCAAGGCCaaa                       >  2:529568/1‑92 (MQ=255)

                                                                  aaaCCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAAc                       >  2:4664375/1‑93 (MQ=255)

                                                                  aaaCCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAAc                       >  1:3320484/1‑93 (MQ=255)

                                                                   aaCCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACg                      >  2:1647219/1‑93 (MQ=255)

                                                                    aCCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGc                     <  1:2449427/93‑1 (MQ=255)

                                                                    aCCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGc                     >  1:4183687/1‑93 (MQ=255)

                                                                      ccTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCg                   >  1:2950793/1‑93 (MQ=255)

                                                                        tttCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCggg                 >  1:3330876/1‑93 (MQ=255)

                                                                          tCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGaa               >  2:2213801/1‑93 (MQ=255)

                                                                          tCACTTGCCTAAAGCCGCGAAGGGTAACATCTGCGGCTTTTTCAATACCAAAAGCCCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGaa               <  2:1455130/93‑1 (MQ=255)

                                                                            aCTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATa             >  1:1604112/1‑93 (MQ=255)

                                                                             cTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATag            >  2:1192505/1‑93 (MQ=255)

                                                                              ttGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATaga           >  2:4322032/1‑93 (MQ=255)

                                                                              ttGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATaga           <  2:4515079/93‑1 (MQ=255)

                                                                                gCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAgg         <  2:333213/93‑1 (MQ=255)

                                                                                gCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAgg         >  2:2370254/1‑93 (MQ=255)

                                                                                gCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAgg         >  2:4448609/1‑93 (MQ=255)

                                                                                 cTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGc        <  1:252037/93‑1 (MQ=255)

                                                                                 cTTAACGACGCGACGGTTAACATCTGCTTCTGTTTCAATACCAAAAGGCCGTACGGCCGCTTTACTCGCCGTGCTAACGCCGGGAATAGAGGc        <  2:1340701/93‑1 (MQ=255)

                                                                                  ttAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCa       >  2:1221371/1‑93 (MQ=255)

                                                                                  ttAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCa       <  2:3527605/93‑1 (MQ=255)

                                                                                  ttAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCa       >  2:2751281/1‑93 (MQ=255)

                                                                                   tAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCaa      <  2:4025197/93‑1 (MQ=255)

                                                                                     aCGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACa    >  2:655346/1‑93 (MQ=255)

                                                                                     aCGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACa    <  2:1998971/93‑1 (MQ=255)

                                                                                       gCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATc  >  1:703050/1‑93 (MQ=255)

CCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATC  >  NC_000913/2147274‑2147453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATA  >  NC_000913/2147274‑2147457

CCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGA                                                                                            <  SRR4302535.3555335/94‑1 (MQ=60)

 CTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGAC                                                                                           >  SRR4302535.1957942/1‑94 (MQ=60)

 CTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGAC                                                                                           >  SRR4302535.3693235/1‑94 (MQ=60)

 CTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGAC                                                                                           <  SRR4302535.1004486/94‑1 (MQ=60)

 CTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGAC                                                                                           <  SRR4302535.3195101/94‑1 (MQ=60)

 CTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGAC                                                                                           <  SRR4302535.1466274/94‑1 (MQ=60)

  TGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACG                                                                                          >  SRR4302535.3357869/1‑94 (MQ=60)

  CGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACG                                                                                          <  SRR4302535.1903387/94‑1 (MQ=60)

   GAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGG                                                                                         <  SRR4302535.1869863/94‑1 (MQ=60)

      CACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTA                                                                                      >  SRR4302535.3235386/1‑94 (MQ=60)

         AAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACA                                                                                   >  SRR4302535.736189/1‑94 (MQ=60)

          AAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACAT                                                                                  >  SRR4302535.696405/1‑94 (MQ=60)

          AAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACAT                                                                                  <  SRR4302535.486460/94‑1 (MQ=60)

          AAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACAT                                                                                  >  SRR4302535.1122532/1‑94 (MQ=60)

          AAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACAT                                                                                  <  SRR4302535.1066915/94‑1 (MQ=60)

            ACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCT                                                                                <  SRR4302535.120143/94‑1 (MQ=60)

            ACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCT                                                                                <  SRR4302535.3146042/94‑1 (MQ=60)

             CGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGGTGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTG                                                                               >  SRR4302535.791440/1‑94 (MQ=60)

              GGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGC                                                                              >  SRR4302535.3977293/1‑94 (MQ=60)

              GGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGC                                                                              >  SRR4302535.3700403/1‑94 (MQ=60)

               GCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCG                                                                             <  SRR4302535.1605270/94‑1 (MQ=60)

               GCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCG                                                                             <  SRR4302535.778107/94‑1 (MQ=60)

                 GTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGC                                                                           >  SRR4302535.1655864/1‑94 (MQ=60)

                  TTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCT                                                                          <  SRR4302535.3721099/94‑1 (MQ=60)

                    cacggatatgtttgcagccgataacccgctaGGTCAGATGGTCTGCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGT                                                                        <  SRR4302535.2754679/63‑1 (MQ=60)

                    CACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGT                                                                        <  SRR4302535.3455872/94‑1 (MQ=60)

                    CACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGT                                                                        <  SRR4302535.2754680/94‑1 (MQ=60)

                          TCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAAT                                                                  >  SRR4302535.224994/1‑94 (MQ=60)

                           CGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATA                                                                 >  SRR4302535.1480694/1‑94 (MQ=60)

                             CTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACC                                                               >  SRR4302535.3150874/1‑94 (MQ=60)

                                TCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAA                                                            >  SRR4302535.2342587/1‑94 (MQ=60)

                                  CAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAG                                                          <  SRR4302535.2453558/94‑1 (MQ=60)

                                  CAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAG                                                          <  SRR4302535.396596/94‑1 (MQ=60)

                                  CAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAG                                                          <  SRR4302535.1312201/94‑1 (MQ=60)

                                  CAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAG                                                          <  SRR4302535.53748/94‑1 (MQ=60)

                                        ATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTA                                                    >  SRR4302535.1239865/1‑94 (MQ=60)

                                        ATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTA                                                    >  SRR4302535.2391405/1‑94 (MQ=60)

                                         TAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAA                                                   >  SRR4302535.1567869/1‑94 (MQ=60)

                                            CCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGC                                                <  SRR4302535.2095907/94‑1 (MQ=60)

                                             CGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCC                                               <  SRR4302535.4690477/94‑1 (MQ=60)

                                             CGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCC                                               <  SRR4302535.1154437/94‑1 (MQ=60)

                                               CCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGATCGTAACGCCGA                                             >  SRR4302535.2189688/1‑94 (MQ=60)

                                                 TGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTT                                           >  SRR4302535.3872769/1‑94 (MQ=60)

                                                  GGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTT                                          >  SRR4302535.1942188/1‑94 (MQ=60)

                                                    GTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTAC                                        >  SRR4302535.3549837/1‑94 (MQ=60)

                                                      CAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGC                                      >  SRR4302535.1037942/1‑94 (MQ=60)

                                                       AGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCG                                     >  SRR4302535.2971598/1‑94 (MQ=60)

                                                       AGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCG                                     <  SRR4302535.3002388/94‑1 (MQ=60)

                                                             TCGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGC                               >  SRR4302535.4014659/1‑94 (MQ=60)

                                                              CGCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCC                              >  SRR4302535.1759556/1‑94 (MQ=60)

                                                               GCCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCA                             >  SRR4302535.1934790/1‑94 (MQ=60)

                                                                CCAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACACCGCTTTACGCGCAGGGACAA                            >  SRR4302535.1453155/1‑94 (MQ=60)

                                                                 CAAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAAC                           >  SRR4302535.3343587/1‑94 (MQ=60)

                                                                  AAACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACG                          >  SRR4302535.3572969/1‑94 (MQ=60)

                                                                   AACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGC                         >  SRR4302535.4212313/1‑94 (MQ=60)

                                                                    ACCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCC                        <  SRR4302535.2467077/94‑1 (MQ=60)

                                                                     CCCTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCG                       >  SRR4302535.2971522/1‑94 (MQ=60)

                                                                       CTTTCACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGG                     >  SRR4302535.3354062/1‑94 (MQ=60)

                                                                           CACTTGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATA                 >  SRR4302535.1615683/1‑94 (MQ=60)

                                                                               TGCTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGG             <  SRR4302535.1712891/94‑1 (MQ=60)

                                                                                 CTTAACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCA           <  SRR4302535.253773/94‑1 (MQ=60)

                                                                                    AACGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACA        <  SRR4302535.1147115/94‑1 (MQ=60)

                                                                                      CGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATC      <  SRR4302535.2703187/94‑1 (MQ=60)

                                                                                      CGCCGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATC      >  SRR4302535.707980/1‑94 (MQ=60)

                                                                                         CGCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAAT   <  SRR4302535.4577349/94‑1 (MQ=60)

                                                                                          GCGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATA  <  SRR4302535.3442757/94‑1 (MQ=60)

CCTGAACACAAAACGGCGTTCACAGCTCGCTTTCCAGTCGATAACCGCCTGGGTCAGATGGTCGCCAAACCCTTTCACTTGCTTAACGCCACGACGGGTAACATCTGCGGCTGTTTCAATACCAAAAGACCGTAACGCCGCTTTACGCGCAGGGCCAACGCCGGGAATAGAGGCAACATCAATA  >  NC_000913/2147274‑2147457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`