Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,165,874 T→C L50L (TTA→TTG)  b2084 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,165,8740TC100.0% 467.5 / NA 147L50L (TTA→TTG) b2084hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (85/62);  total (85/62)

Only 100 of 149 total aligned reads displayed.

TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG  >  NC_000913/2165784‑2165961
                                                                                          |                                                                                       
tCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCaa                                                                                       <  1:4277624/93‑1 (MQ=255)
 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                      >  2:488177/1‑93 (MQ=255)
 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                      >  2:4432441/1‑93 (MQ=255)
   gtgtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCg                                                                                    <  2:1218260/93‑1 (MQ=255)
   gtgtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCg                                                                                    >  2:2738568/1‑93 (MQ=255)
    tgtAAATATCTGCCCGCCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGt                                                                                   <  1:1644426/93‑1 (MQ=255)
     gtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTc                                                                                  >  2:1703164/1‑93 (MQ=255)
     gtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTc                                                                                  >  1:4309658/1‑93 (MQ=255)
        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                               <  2:1495902/93‑1 (MQ=255)
        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                               <  1:3516428/93‑1 (MQ=255)
        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                               <  2:1076076/93‑1 (MQ=255)
         atatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTcc                                                                              >  2:317585/1‑93 (MQ=255)
          tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCa                                                                             <  2:2125428/93‑1 (MQ=255)
          tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCa                                                                             <  2:393674/93‑1 (MQ=255)
          tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCa                                                                             <  1:3153025/93‑1 (MQ=255)
           atCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAt                                                                            <  1:2815308/93‑1 (MQ=255)
              tGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGa                                                                         <  1:1839285/93‑1 (MQ=255)
                cccGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAcc                                                                       >  2:657208/1‑93 (MQ=255)
                 ccGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACca                                                                      >  2:3847989/1‑93 (MQ=255)
                 ccGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACca                                                                      >  1:1789808/1‑93 (MQ=255)
                    aCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAt                                                                   >  2:2985742/1‑93 (MQ=255)
                    aCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAt                                                                   >  2:4309214/1‑93 (MQ=255)
                    aCCGGTAGCCAGGCTGATGGACAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAt                                                                   >  1:1824293/1‑93 (MQ=255)
                     ccGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATc                                                                  <  1:2143481/93‑1 (MQ=255)
                     ccGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATc                                                                  <  1:2355024/93‑1 (MQ=255)
                     ccGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATc                                                                  <  1:724372/93‑1 (MQ=255)
                       ggTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTTGCAACCGTCTTCCATTGACCACATCGa                                                                <  1:3377128/93‑1 (MQ=255)
                       ggTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGa                                                                <  2:2049381/93‑1 (MQ=255)
                       ggTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGa                                                                >  2:1928527/1‑93 (MQ=255)
                       ggTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTGTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGa                                                                <  2:2049380/93‑1 (MQ=255)
                        gTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAt                                                               >  1:926629/1‑93 (MQ=255)
                           gCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGa                                                            <  1:3008856/93‑1 (MQ=255)
                           gCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGa                                                            >  1:835494/1‑93 (MQ=255)
                           gCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGa                                                            >  1:4641788/1‑93 (MQ=255)
                            ccAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGaa                                                           >  1:3938300/1‑93 (MQ=255)
                            ccAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGaa                                                           <  1:258699/93‑1 (MQ=255)
                              aGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtc                                                         <  2:1901287/93‑1 (MQ=255)
                              aGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtc                                                         <  1:206770/93‑1 (MQ=255)
                              aGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtc                                                         >  2:4322038/1‑93 (MQ=255)
                               ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcc                                                        <  1:1076844/93‑1 (MQ=255)
                                   gATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAGTcctcct                                                    >  1:3149815/1‑93 (MQ=255)
                                   gATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATcctcct                                                    >  1:1767719/1‑93 (MQ=255)
                                    aTGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCtt                                                   <  1:2665733/93‑1 (MQ=255)
                                       gagaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCAt                                                <  2:3581048/93‑1 (MQ=255)
                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                               <  1:2527624/93‑1 (MQ=255)
                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                               <  1:3271788/93‑1 (MQ=255)
                                         gaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAg                                              >  2:3847881/1‑93 (MQ=255)
                                         gaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAg                                              <  2:4425143/93‑1 (MQ=255)
                                           aCTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCa                                            >  1:52427/1‑93 (MQ=255)
                                           aCTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCa                                            <  2:438713/93‑1 (MQ=255)
                                           aCTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCa                                            <  2:4390312/93‑1 (MQ=255)
                                             tctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACg                                          >  2:3242113/1‑93 (MQ=255)
                                             tctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACg                                          >  2:4263954/1‑93 (MQ=255)
                                              ctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGa                                         <  1:2331322/93‑1 (MQ=255)
                                              ctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGa                                         <  1:4195167/93‑1 (MQ=255)
                                                 cTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGc                                      <  2:2627044/93‑1 (MQ=255)
                                                  tACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGcc                                     >  2:3319844/1‑93 (MQ=255)
                                                  tACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGcc                                     >  1:4489989/1‑93 (MQ=255)
                                                  tACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGcc                                     >  1:3593796/1‑93 (MQ=255)
                                                   acacGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCt                                    >  2:684456/1‑93 (MQ=255)
                                                   acacGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCt                                    >  2:2464466/1‑93 (MQ=255)
                                                     acGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCttt                                  >  2:3528920/1‑93 (MQ=255)
                                                        ggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGACAGAATCCTCCTTCATAGCAAGATGCCTTTCAc                               >  2:3024475/1‑93 (MQ=255)
                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                            >  2:935595/1‑93 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                          <  2:1214928/93‑1 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                          >  2:23708/1‑93 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                          >  2:773801/1‑93 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                          <  1:933155/93‑1 (MQ=255)
                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                          <  2:3741455/93‑1 (MQ=255)
                                                              tttttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCg                         >  2:3577847/1‑93 (MQ=255)
                                                                tttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGc                       <  2:1597017/93‑1 (MQ=255)
                                                                 ttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCa                      <  2:3994159/93‑1 (MQ=255)
                                                                 ttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCa                      <  1:2648483/93‑1 (MQ=255)
                                                                  tttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAt                     <  2:3191980/93‑1 (MQ=255)
                                                                   ttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATc                    <  1:961013/93‑1 (MQ=255)
                                                                    tATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCg                   >  1:1836165/1‑93 (MQ=255)
                                                                      tGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTg                 >  2:2421275/1‑93 (MQ=255)
                                                                       gCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGc                >  1:4095564/1‑93 (MQ=255)
                                                                       gCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGc                <  1:4422235/93‑1 (MQ=255)
                                                                       gCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGc                >  2:4233848/1‑93 (MQ=255)
                                                                         aTAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCGTCGTGCTc              >  1:4618214/1‑93 (MQ=255)
                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc             >  1:1032539/1‑93 (MQ=255)
                                                                              cccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCcaca         >  1:3284846/1‑93 (MQ=255)
                                                                              cccTATCTATGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCcaca         <  1:2306736/93‑1 (MQ=255)
                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg        >  1:584958/1‑93 (MQ=255)
                                                                               ccTATCTCTGGCAACCCTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg        <  2:4526093/93‑1 (MQ=255)
                                                                                cTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg       >  1:2995506/1‑93 (MQ=255)
                                                                                cTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg       >  1:3869901/1‑93 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt      >  1:2085488/1‑93 (MQ=255)
                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt      <  2:985965/93‑1 (MQ=255)
                                                                                  aTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgggt     >  2:1695939/1‑91 (MQ=255)
                                                                                  aTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGtt     >  2:3447636/1‑93 (MQ=255)
                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc    >  2:3812229/1‑93 (MQ=255)
                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc    >  1:1750153/1‑93 (MQ=255)
                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc    >  1:2939398/1‑93 (MQ=255)
                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc    >  1:1543876/1‑93 (MQ=255)
                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc    >  1:1301080/1‑93 (MQ=255)
                                                                                    ctctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTcc   <  2:1090760/93‑1 (MQ=255)
                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg  <  1:3409016/93‑1 (MQ=255)
                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGAACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg  >  2:942262/1‑93 (MQ=255)
                                                                                          |                                                                                       
TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG  >  NC_000913/2165784‑2165961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  NC_000913/2165781‑2165967
                                                                                             |                                                                                             
GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGC                                                                                               <  SRR4302535.1617678/94‑1 (MQ=60)
  TTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAA                                                                                             >  SRR4302535.1697096/1‑94 (MQ=60)
   TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAC                                                                                            <  SRR4302535.4306900/94‑1 (MQ=60)
      GTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGT                                                                                         >  SRR4302535.3260791/1‑94 (MQ=60)
       TGTAAATATCTGCCCGCCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTC                                                                                        <  SRR4302535.1656312/94‑1 (MQ=60)
       TGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTC                                                                                        >  SRR4302535.4339141/1‑94 (MQ=60)
        GTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCT                                                                                       >  SRR4302535.2988317/1‑94 (MQ=60)
           AATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCC                                                                                    <  SRR4302535.3540861/94‑1 (MQ=60)
             TATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAT                                                                                  <  SRR4302535.3174934/94‑1 (MQ=60)
              ATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATT                                                                                 <  SRR4302535.2835309/94‑1 (MQ=60)
                 TGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAC                                                                              <  SRR4302535.1852551/94‑1 (MQ=60)
                   CCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCA                                                                            >  SRR4302535.1802755/1‑94 (MQ=60)
                     CGCCCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACA                                                                          <  SRR4302535.4054444/94‑1 (MQ=60)
                      GACCGGTAGCCAGGCTGATGGACAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAT                                                                         >  SRR4302535.1837465/1‑94 (MQ=60)
                       ACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATC                                                                        >  SRR4302535.143636/1‑94 (MQ=60)
                        CCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCG                                                                       <  SRR4302535.2371909/94‑1 (MQ=60)
                        CCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCG                                                                       <  SRR4302535.729455/94‑1 (MQ=60)
                        CCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCG                                                                       <  SRR4302535.2158899/94‑1 (MQ=60)
                          GGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTTGCAACCGTCTTCCATTGACCACATCGAT                                                                     <  SRR4302535.3400611/94‑1 (MQ=60)
                          GGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAT                                                                     <  SRR4302535.3357544/94‑1 (MQ=60)
                          GGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAT                                                                     >  SRR4302535.933192/1‑94 (MQ=60)
                             AGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGA                                                                  >  SRR4302535.4673254/1‑94 (MQ=60)
                             AGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGA                                                                  >  SRR4302535.841353/1‑94 (MQ=60)
                              GCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAA                                                                 >  SRR4302535.3965179/1‑94 (MQ=60)
                              GCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAA                                                                 >  SRR4302535.4347695/1‑94 (MQ=60)
                              GCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAA                                                                 <  SRR4302535.3029931/94‑1 (MQ=60)
                               CCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAT                                                                <  SRR4302535.260489/94‑1 (MQ=60)
                                 AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCC                                                              <  SRR4302535.208189/94‑1 (MQ=60)
                                 AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCC                                                              <  SRR4302535.1858275/94‑1 (MQ=60)
                                 AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCC                                                              >  SRR4302535.1839168/1‑94 (MQ=60)
                                  GGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCT                                                             <  SRR4302535.1084502/94‑1 (MQ=60)
                                     TGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAGTCCTCCT                                                          >  SRR4302535.3171709/1‑94 (MQ=60)
                                     TGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCT                                                          >  SRR4302535.2107010/1‑94 (MQ=60)
                                     TGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCT                                                          >  SRR4302535.656656/1‑94 (MQ=60)
                                     TGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCT                                                          >  SRR4302535.1780537/1‑94 (MQ=60)
                                       ATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTC                                                        <  SRR4302535.2684819/94‑1 (MQ=60)
                                           AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    <  SRR4302535.2545833/94‑1 (MQ=60)
                                           AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    <  SRR4302535.3294516/94‑1 (MQ=60)
                                             AACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCA                                                  >  SRR4302535.52809/1‑94 (MQ=60)
                                              ACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCAC                                                 >  SRR4302535.1797773/1‑94 (MQ=60)
                                                 CTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGAT                                              <  SRR4302535.4223874/94‑1 (MQ=60)
                                                 CTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGAT                                              <  SRR4302535.2347995/94‑1 (MQ=60)
                                                    CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           >  SRR4302535.3618737/1‑94 (MQ=60)
                                                    CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           >  SRR4302535.4520809/1‑94 (MQ=60)
                                                             CTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTA                                  <  SRR4302535.3490580/94‑1 (MQ=60)
                                                              TTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAT                                 <  SRR4302535.2660345/94‑1 (MQ=60)
                                                               TATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATC                                >  SRR4302535.4300404/1‑94 (MQ=60)
                                                                TTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCG                               <  SRR4302535.94961/94‑1 (MQ=60)
                                                                ATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCG                               >  SRR4302535.4589414/1‑94 (MQ=60)
                                                                ATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCG                               <  SRR4302535.939755/94‑1 (MQ=60)
                                                                  TTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGC                             <  SRR4302535.91518/94‑1 (MQ=60)
                                                                    TTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAT                           <  SRR4302535.2667453/94‑1 (MQ=60)
                                                                    TTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAT                           >  SRR4302535.2326590/1‑94 (MQ=60)
                                                                      TTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCG                         <  SRR4302535.967840/94‑1 (MQ=60)
                                                                      TTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCG                         >  SRR4302535.1849410/1‑94 (MQ=60)
                                                                         TGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGC                      >  SRR4302535.4123596/1‑94 (MQ=60)
                                                                          GCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCT                     <  SRR4302535.4452521/94‑1 (MQ=60)
                                                                           CATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCGTCGTGCTC                    >  SRR4302535.4649597/1‑94 (MQ=60)
                                                                            ATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCC                   >  SRR4302535.2692597/1‑94 (MQ=60)
                                                                            ATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCC                   >  SRR4302535.1039875/1‑94 (MQ=60)
                                                                              AAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCA                 >  SRR4302535.3767086/1‑94 (MQ=60)
                                                                                GCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACA               >  SRR4302535.3307679/1‑94 (MQ=60)
                                                                                 CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              >  SRR4302535.589058/1‑94 (MQ=60)
                                                                                 CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              >  SRR4302535.3101131/1‑94 (MQ=60)
                                                                                 CCCTATCTATGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              <  SRR4302535.2323233/94‑1 (MQ=60)
                                                                                  CCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGG             >  SRR4302535.3896500/1‑94 (MQ=60)
                                                                                  CCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGG             >  SRR4302535.3016504/1‑94 (MQ=60)
                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302535.2100507/1‑94 (MQ=60)
                                                                                    TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           >  SRR4302535.497550/1‑94 (MQ=60)
                                                                                    TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           <  SRR4302535.2425246/94‑1 (MQ=60)
                                                                                     ATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTC          >  SRR4302535.1762811/1‑94 (MQ=60)
                                                                                     ATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTC          >  SRR4302535.1555001/1‑94 (MQ=60)
                                                                                     ATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTC          >  SRR4302535.1310358/1‑94 (MQ=60)
                                                                                     ATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTC          >  SRR4302535.2960071/1‑94 (MQ=60)
                                                                                        TCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGG       <  SRR4302535.3432742/94‑1 (MQ=60)
                                                                                           GGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTA    >  SRR4302535.648820/1‑94 (MQ=60)
                                                                                           GGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTA    >  SRR4302535.2678582/1‑94 (MQ=60)
                                                                                            GCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTAC   <  SRR4302535.2379340/94‑1 (MQ=60)
                                                                                             CAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  <  SRR4302535.1582380/94‑1 (MQ=60)
                                                                                             CAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  SRR4302535.1840149/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  NC_000913/2165781‑2165967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑