Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,165,874	T→C	L50L (TTA→TTG)	b2084 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,165,874	0	T	C	99.3%	864.6 / NA	273	L50L (TTA→TTG)	b2084	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (154/117); minor base A (1/1); total (155/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC  >  NC_000913/2165784‑2165963

tCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCaa                                                                                         >  1:392713/1‑93 (MQ=255)

tCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCaa                                                                                         <  2:5455870/93‑1 (MQ=255)

 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTCCACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        <  1:3385701/93‑1 (MQ=255)

 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        >  2:3632871/1‑93 (MQ=255)

 ccGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        <  1:550575/93‑1 (MQ=255)

 ccGTGTAAAGATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAAc                                                                                        <  1:6645481/93‑1 (MQ=255)

     gtAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTc                                                                                    <  1:115047/93‑1 (MQ=255)

      tAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCt                                                                                   >  2:4515040/1‑93 (MQ=255)

      tAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCt                                                                                   >  1:6142024/1‑93 (MQ=255)

       aaaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCtt                                                                                  <  2:1477981/93‑1 (MQ=255)

        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                                 <  1:4920801/93‑1 (MQ=255)

        aaTATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTc                                                                                 >  1:1236208/1‑93 (MQ=255)

          tatCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCa                                                                               <  1:7269135/93‑1 (MQ=255)

           atCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAt                                                                              <  1:3231857/93‑1 (MQ=255)

            tCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAtt                                                                             >  1:7786394/1‑93 (MQ=255)

              tGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGa                                                                           >  2:404183/1‑93 (MQ=255)

               gCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAc                                                                          >  2:6911488/1‑93 (MQ=255)

               gCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAc                                                                          <  1:6001028/93‑1 (MQ=255)

                cccGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAcc                                                                         >  2:1688868/1‑93 (MQ=255)

                      cGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCg                                                                   >  2:3879793/1‑93 (MQ=255)

                        gTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAt                                                                 >  2:6212903/1‑93 (MQ=255)

                        gTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAt                                                                 >  1:5643876/1‑93 (MQ=255)

                         tAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATa                                                                <  1:4096227/93‑1 (MQ=255)

                         tAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATa                                                                >  2:2128403/1‑93 (MQ=255)

                          aGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAg                                                               <  1:2976320/93‑1 (MQ=255)

                          aGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAg                                                               >  1:5124024/1‑93 (MQ=255)

                           gCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGa                                                              >  1:3655733/1‑93 (MQ=255)

                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            >  1:2477190/1‑93 (MQ=255)

                             cAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAt                                                            >  2:4317652/1‑93 (MQ=255)

                              aGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtc                                                           <  2:4264656/93‑1 (MQ=255)

                               ggCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAGtcc                                                          >  1:6235430/1‑93 (MQ=255)

                               agCTCATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATACGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcc                                                          <  1:4352066/92‑1 (MQ=255)

                                  tGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcctcc                                                       <  1:3492657/93‑1 (MQ=255)

                                  tGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAtcctcc                                                       <  2:7496187/93‑1 (MQ=255)

                                   gATGGAGAACTCTGCTACACGGGCTTATTGTTGTATGCATAAGCCCTATCTCTGGCAACCGTCTGCCAGCGACCACAGCGATAGAATcctcct                                                      >  1:7027721/1‑93 (MQ=255)

                                     tGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTc                                                    <  2:7203116/93‑1 (MQ=255)

                                      ggAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCa                                                   <  1:6570440/93‑1 (MQ=255)

                                      ggAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCa                                                   >  2:6737685/1‑93 (MQ=255)

                                       gagaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAACACATCGATAGAATCCTCCTTCAt                                                  <  1:1062202/93‑1 (MQ=255)

                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                                 <  2:7178219/93‑1 (MQ=255)

                                        agaACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATa                                                 >  2:4863497/1‑93 (MQ=255)

                                            ctctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCAc                                             <  1:6297105/93‑1 (MQ=255)

                                              ctGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGa                                           <  2:5533150/93‑1 (MQ=255)

                                                gCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATg                                         <  1:2026025/93‑1 (MQ=255)

                                                   acacGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCt                                      <  2:4730718/93‑1 (MQ=255)

                                                    cacGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCtt                                     <  2:548662/93‑1 (MQ=255)

                                                    cacGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCtt                                     <  2:3581886/93‑1 (MQ=255)

                                                     acGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCttt                                    <  2:5426394/93‑1 (MQ=255)

                                                     acGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCttt                                    >  1:2785874/1‑93 (MQ=255)

                                                      cGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTc                                   <  1:2190413/93‑1 (MQ=255)

                                                      cGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTc                                   >  2:5808407/1‑93 (MQ=255)

                                                       gggCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCa                                  >  1:3170205/1‑93 (MQ=255)

                                                         gCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACt                                <  2:732737/93‑1 (MQ=255)

                                                          cTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACtt                               <  2:3400745/93‑1 (MQ=255)

                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              >  1:7187657/1‑93 (MQ=255)

                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              <  2:2869979/93‑1 (MQ=255)

                                                           ttATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTa                              <  1:2345141/93‑1 (MQ=255)

                                                             aTTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATc                            >  2:4626838/1‑93 (MQ=255)

                                                              tttttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCg                           >  2:517238/1‑93 (MQ=255)

                                                                tttttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGc                         >  2:1550668/1‑93 (MQ=255)

                                                                  tttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAt                       >  1:4654350/1‑93 (MQ=255)

                                                                   ttATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATc                      >  2:2112337/1‑93 (MQ=255)

                                                                   ttATGCATAAGCCCTATCTCTGGCAACCATCTTCCATTGACCACATCGCTAGAATTCCCCTTCATAGCACGATGCCTTTCACTTATCGGCATc                      >  2:5045137/1‑93 (MQ=255)

                                                                    tATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCg                     >  2:4210297/1‑93 (MQ=255)

                                                                     aTGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATATAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGt                    >  1:6983164/1‑93 (MQ=255)

                                                                     aTGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGt                    >  1:4916030/1‑93 (MQ=255)

                                                                      tGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTg                   >  2:117885/1‑93 (MQ=255)

                                                                      tGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTg                   <  2:3209693/93‑1 (MQ=255)

                                                                        cATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCt                 >  2:5897634/1‑93 (MQ=255)

                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               >  1:5621171/1‑93 (MQ=255)

                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               >  2:2699009/1‑93 (MQ=255)

                                                                          tAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTcc               >  1:1786528/1‑93 (MQ=255)

                                                                            aGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCca             >  2:4833277/1‑93 (MQ=255)

                                                                             gCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCcac            >  1:6535452/1‑93 (MQ=255)

                                                                              cccTTTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCcaca           <  1:6779727/93‑1 (MQ=255)

                                                                              cccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCcaca           >  1:4857376/1‑93 (MQ=255)

                                                                              cccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGCGCTCCcaca           <  1:3891126/93‑1 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATATCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  2:7128074/1‑93 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          <  2:3376901/93‑1 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  2:4108397/1‑93 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  1:845398/1‑93 (MQ=255)

                                                                               ccTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAg          >  1:950791/1‑93 (MQ=255)

                                                                                cTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg         >  2:7733798/1‑93 (MQ=255)

                                                                                cTATCTCTGACAACCGTCTTCCATTGGCCACATCGATAGCATCCTCCTACATAGCCCGATGCCTTTCACTTATCGGCATCGTGCTCCCACAgg         <  2:7029587/93‑1 (MQ=255)

                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        <  2:2453406/93‑1 (MQ=255)

                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  2:6131308/1‑93 (MQ=255)

                                                                                 tATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGt        >  2:1360119/1‑93 (MQ=255)

                                                                                  aTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGtt       >  1:4450578/1‑93 (MQ=255)

                                                                                  aTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGtt       <  2:2359823/93‑1 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      <  2:2897937/93‑1 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      >  1:4595644/1‑93 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      >  1:7459124/1‑93 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      >  2:1265404/1‑93 (MQ=255)

                                                                                   tctcTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTc      >  2:1933551/1‑93 (MQ=255)

                                                                                    ctctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTcc     <  1:5832976/93‑1 (MQ=255)

                                                                                    ctctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTcc     >  1:1922158/1‑93 (MQ=255)

                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCTGCATCGTGCTCCCACACGTTCCg    >  1:3524905/1‑93 (MQ=255)

                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg    >  2:6351526/1‑93 (MQ=255)

                                                                                     tctGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCg    <  2:4444960/93‑1 (MQ=255)

                                                                                       tGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGc  >  1:4258878/1‑93 (MQ=255)

TCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC  >  NC_000913/2165784‑2165963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  NC_000913/2165781‑2165967

GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGC                                                                                               >  SRR4302543.7949999/1‑94 (MQ=60)

  TTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAA                                                                                             >  SRR4302543.396336/1‑94 (MQ=60)

  TTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAA                                                                                             <  SRR4302543.5776911/94‑1 (MQ=60)

    CCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTCCACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACC                                                                                           <  SRR4302543.3416059/94‑1 (MQ=60)

    CCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACC                                                                                           <  SRR4302543.1179171/94‑1 (MQ=60)

    CCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACC                                                                                           <  SRR4302543.555642/94‑1 (MQ=60)

    CCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACC                                                                                           <  SRR4302543.6708201/94‑1 (MQ=60)

    CCGTGTAAAGATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACC                                                                                           <  SRR4302543.6708202/94‑1 (MQ=60)

      GTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGT                                                                                         >  SRR4302543.4994005/1‑94 (MQ=60)

        GTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCT                                                                                       >  SRR4302543.6200199/1‑94 (MQ=60)

        GTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCT                                                                                       <  SRR4302543.116089/94‑1 (MQ=60)

          AAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTC                                                                                     >  SRR4302543.1247335/1‑94 (MQ=60)

           AATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCC                                                                                    <  SRR4302543.4346115/94‑1 (MQ=60)

           AATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCC                                                                                    <  SRR4302543.4965634/94‑1 (MQ=60)

           AATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCC                                                                                    >  SRR4302543.6692924/1‑94 (MQ=60)

           AATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCC                                                                                    <  SRR4302543.4965633/94‑1 (MQ=60)

             TATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAT                                                                                  <  SRR4302543.4552367/94‑1 (MQ=60)

             TATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAT                                                                                  <  SRR4302543.7339364/94‑1 (MQ=60)

             TATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCAT                                                                                  <  SRR4302543.7339365/94‑1 (MQ=60)

              ATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATT                                                                                 <  SRR4302543.3261086/94‑1 (MQ=60)

              ATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATT                                                                                 >  SRR4302543.4059569/1‑94 (MQ=60)

              ATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATT                                                                                 >  SRR4302543.7859800/1‑94 (MQ=60)

              ATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATT                                                                                 >  SRR4302543.7859799/1‑94 (MQ=60)

              ATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGCAAACCGTCTTCCATT                                                                                 >  SRR4302543.4059571/1‑94 (MQ=60)

               TCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTG                                                                                <  SRR4302543.1805381/94‑1 (MQ=60)

               TCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTG                                                                                <  SRR4302543.7477208/94‑1 (MQ=60)

                  GCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACC                                                                             <  SRR4302543.4946765/94‑1 (MQ=60)

                  GCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACC                                                                             <  SRR4302543.6056173/94‑1 (MQ=60)

                   CCCGACCGGTAGCCAGGCTGTTGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACTGCCTTCCATTGACCA                                                                            >  SRR4302543.7439997/1‑94 (MQ=60)

                    CCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCAC                                                                           <  SRR4302543.3639768/94‑1 (MQ=60)

                     CGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACA                                                                          >  SRR4302543.3228447/1‑94 (MQ=60)

                     CGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACA                                                                          >  SRR4302543.239872/1‑94 (MQ=60)

                     CGACCGGTAGCCAGACTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACA                                                                          <  SRR4302543.2782097/94‑1 (MQ=60)

                      GACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACAT                                                                         <  SRR4302543.2301869/94‑1 (MQ=60)

                          GGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATACGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAT                                                                     <  SRR4302543.680560/94‑1 (MQ=60)

                          GGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAT                                                                     <  SRR4302543.3024793/94‑1 (MQ=60)

                          GGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGAT                                                                     >  SRR4302543.5695323/1‑94 (MQ=60)

                           GTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATA                                                                    <  SRR4302543.627582/94‑1 (MQ=60)

                            TAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAG                                                                   <  SRR4302543.4133003/94‑1 (MQ=60)

                            TAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAG                                                                   >  SRR4302543.5170353/1‑94 (MQ=60)

                            GAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAG                                                                   <  SRR4302543.5872752/94‑1 (MQ=60)

                             AGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGA                                                                  >  SRR4302543.3290794/1‑94 (MQ=60)

                             AGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGA                                                                  >  SRR4302543.3687843/1‑94 (MQ=60)

                             AGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGA                                                                  <  SRR4302543.3003349/94‑1 (MQ=60)

                               CCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAT                                                                >  SRR4302543.2499590/1‑94 (MQ=60)

                               CCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAAT                                                                >  SRR4302543.2140284/1‑94 (MQ=60)

                                 AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAGTCC                                                              >  SRR4302543.6294279/1‑94 (MQ=60)

                                 AGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCC                                                              <  SRR4302543.3238078/94‑1 (MQ=60)

                                  agctcATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATACGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCT                                                             <  SRR4302543.4391512/89‑1 (MQ=60)

                                    CTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCC                                                           >  SRR4302543.1100141/1‑94 (MQ=60)

                                    CTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCC                                                           >  SRR4302543.3686769/1‑94 (MQ=60)

                                    CTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCC                                                           >  SRR4302543.6627534/1‑94 (MQ=60)

                                     TGATGGAGAACTCTGTTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCT                                                          <  SRR4302543.47942/94‑1 (MQ=60)

                                     TGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCT                                                          <  SRR4302543.3523915/94‑1 (MQ=60)

                                     TGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCT                                                          <  SRR4302543.6981811/94‑1 (MQ=60)

                                     TGATGGAGAACTCTGCTACACGGGCTTATTGTTGTATGCATAAGCCCTATCTCTGGCAACCGTCTGCCAGCGACCACAGCGATAGAATCCTCCT                                                          >  SRR4302543.7095190/1‑94 (MQ=60)

                                      GATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTT                                                         <  SRR4302543.7962131/94‑1 (MQ=60)

                                       ATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTC                                                        >  SRR4302543.3543214/1‑94 (MQ=60)

                                        TGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCA                                                       <  SRR4302543.7219052/94‑1 (MQ=60)

                                        TGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCA                                                       <  SRR4302543.3416963/94‑1 (MQ=60)

                                         GGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCAT                                                      <  SRR4302543.6632296/94‑1 (MQ=60)

                                          GAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGAACACATCGATAGAATCCTCCTTCATA                                                     <  SRR4302543.1071739/94‑1 (MQ=60)

                                           AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    >  SRR4302543.6137486/1‑94 (MQ=60)

                                           AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    >  SRR4302543.6573670/1‑94 (MQ=60)

                                           AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    <  SRR4302543.63337/94‑1 (MQ=60)

                                           AGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAG                                                    <  SRR4302543.4670386/94‑1 (MQ=60)

                                             AACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCA                                                  >  SRR4302543.2459872/1‑94 (MQ=60)

                                               CTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACG                                                <  SRR4302543.6356373/94‑1 (MQ=60)

                                                 CTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGAT                                              <  SRR4302543.6037654/94‑1 (MQ=60)

                                                 CTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGAT                                              <  SRR4302543.4929271/94‑1 (MQ=60)

                                                  TGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATG                                             >  SRR4302543.2970659/1‑94 (MQ=60)

                                                  TGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATG                                             >  SRR4302543.3061815/1‑94 (MQ=60)

                                                   TCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGC                                            <  SRR4302543.6032516/94‑1 (MQ=60)

                                                   GCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGC                                            <  SRR4302543.2044232/94‑1 (MQ=60)

                                                   GCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGC                                            >  SRR4302543.6422485/1‑94 (MQ=60)

                                                    CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           >  SRR4302543.560495/1‑94 (MQ=60)

                                                    CTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCC                                           >  SRR4302543.3845715/1‑94 (MQ=60)

                                                     TACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCT                                          >  SRR4302543.4525691/1‑94 (MQ=60)

                                                     TACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCT                                          >  SRR4302543.5827465/1‑94 (MQ=60)

                                                       CACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTT                                        >  SRR4302543.2811289/1‑94 (MQ=60)

                                                       CACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTT                                        <  SRR4302543.6341420/94‑1 (MQ=60)

                                                       CACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTT                                        >  SRR4302543.3809986/1‑94 (MQ=60)

                                                         CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCA                                      <  SRR4302543.2210082/94‑1 (MQ=60)

                                                         CGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCA                                      >  SRR4302543.3198759/1‑94 (MQ=60)

                                                          GGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCAC                                     >  SRR4302543.4151650/1‑94 (MQ=60)

                                                          GGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTACCTCATAGCACGATGCCTTTCAC                                     >  SRR4302543.5652991/1‑94 (MQ=60)

                                                           GGCTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACT                                    <  SRR4302543.7773948/94‑1 (MQ=60)

                                                             CTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTA                                  >  SRR4302543.437913/1‑94 (MQ=60)

                                                             CTTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTA                                  >  SRR4302543.7257381/1‑94 (MQ=60)

                                                              TTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAT                                 <  SRR4302543.2366415/94‑1 (MQ=60)

                                                              TTATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTAT                                 >  SRR4302543.1970141/1‑94 (MQ=60)

                                                               TATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATC                                >  SRR4302543.2839555/1‑94 (MQ=60)

                                                                ATTTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCG                               >  SRR4302543.5218754/1‑94 (MQ=60)

                                                                  TTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGC                             >  SRR4302543.903118/1‑94 (MQ=60)

                                                                  TTTTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGC                             >  SRR4302543.4776066/1‑94 (MQ=60)

                                                                    TTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAT                           >  SRR4302543.2084639/1‑94 (MQ=60)

                                                                    TTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAT                           >  SRR4302543.4696735/1‑94 (MQ=60)

                                                                    TTTTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCAT                           >  SRR4302543.2211001/1‑94 (MQ=60)

                                                                      TTATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCG                         >  SRR4302543.7897025/1‑94 (MQ=60)

                                                                       TATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATATAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGT                        >  SRR4302543.7048887/1‑94 (MQ=60)

                                                                       TATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATATAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGT                        >  SRR4302543.7048888/1‑94 (MQ=60)

                                                                       TATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGT                        >  SRR4302543.4960822/1‑94 (MQ=60)

                                                                        ATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTG                       >  SRR4302543.1244529/1‑94 (MQ=60)

                                                                        ATGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTG                       <  SRR4302543.6927140/94‑1 (MQ=60)

                                                                        ATGCATAAGCCCTATCTCTGGCAACCGGCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTG                       <  SRR4302543.5681342/94‑1 (MQ=60)

                                                                         TGCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGC                      <  SRR4302543.5356246/94‑1 (MQ=60)

                                                                          GCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCT                     <  SRR4302543.983070/94‑1 (MQ=60)

                                                                          GCATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCT                     <  SRR4302543.1899925/94‑1 (MQ=60)

                                                                            ATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCC                   >  SRR4302543.1802506/1‑94 (MQ=60)

                                                                            ATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCC                   >  SRR4302543.6069047/1‑94 (MQ=60)

                                                                            ATAAGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCC                   >  SRR4302543.5672400/1‑94 (MQ=60)

                                                                               AGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCAC                >  SRR4302543.6596972/1‑94 (MQ=60)

                                                                               AGCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCAC                >  SRR4302543.6596971/1‑94 (MQ=60)

                                                                                GCCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACA               >  SRR4302543.4901667/1‑94 (MQ=60)

                                                                                 CCCTTTCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              <  SRR4302543.6843751/94‑1 (MQ=60)

                                                                                 CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCTCAG              <  SRR4302543.7122353/94‑1 (MQ=60)

                                                                                 CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              >  SRR4302543.959099/1‑94 (MQ=60)

                                                                                 CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              >  SRR4302543.852894/1‑94 (MQ=60)

                                                                                 CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAG              <  SRR4302543.3282137/94‑1 (MQ=60)

                                                                                 CCCTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGCGCTCCCACAG              <  SRR4302543.3925480/94‑1 (MQ=60)

                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302543.3542790/1‑94 (MQ=60)

                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302543.3939814/1‑94 (MQ=60)

                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302543.1313411/1‑94 (MQ=60)

                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302543.170869/1‑94 (MQ=60)

                                                                                   CTATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGT            >  SRR4302543.4672065/1‑94 (MQ=60)

                                                                                    TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           >  SRR4302543.4490884/1‑94 (MQ=60)

                                                                                    TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           >  SRR4302543.4490883/1‑94 (MQ=60)

                                                                                    TATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTT           >  SRR4302543.500652/1‑94 (MQ=60)

                                                                                     ATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTC          >  SRR4302543.4637378/1‑94 (MQ=60)

                                                                                     ATCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTC          >  SRR4302543.7530576/1‑94 (MQ=60)

                                                                                      TCTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCC         >  SRR4302543.1939482/1‑94 (MQ=60)

                                                                                       CTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCTGCATCGTGCTCCCACACGTTCCG        >  SRR4302543.3556334/1‑94 (MQ=60)

                                                                                       CTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG        <  SRR4302543.5886029/94‑1 (MQ=60)

                                                                                       CTCTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCG        >  SRR4302543.4155723/1‑94 (MQ=60)

                                                                                         CTGGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGC      >  SRR4302543.4297548/1‑94 (MQ=60)

                                                                                          TGGCAGCCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCT     <  SRR4302543.131374/94‑1 (MQ=60)

                                                                                           GGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTA    >  SRR4302543.3566000/1‑94 (MQ=60)

                                                                                           GGCAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTA    >  SRR4302543.2487232/1‑94 (MQ=60)

                                                                                           GGCAACAGTCTTCCATTGACCACATGGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTA    >  SRR4302543.2487231/1‑94 (MQ=60)

                                                                                             CAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  SRR4302543.3474436/1‑94 (MQ=60)

                                                                                             CAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  SRR4302543.2253738/1‑94 (MQ=60)

GTTTCCGTGTAAATATCTGCCCGACCGGTAGCCAGGCTGATGGAGAACTCTGCTACACGGGCTTATTTTTTTATGCATAAGCCCTATCTCTGGTAACCGTCTTCCATTGACCACATCGATAGAATCCTCCTTCATAGCACGATGCCTTTCACTTATCGGCATCGTGCTCCCACAGGTTCCGGCTACG  >  NC_000913/2165781‑2165967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`