Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,167,779	T→C	intergenic (+146/‑470)	b2086 → / → b2088	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,167,779	0	T	C	99.6%	764.5 / ‑5.0	243	intergenic (+146/‑470)	b2086/b2088	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (123/119); total (123/120)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01

Only 100 of 259 total aligned reads displayed.

CACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATC  >  NC_000913/2167689‑2167868

cacCTTATCCGGCATACAGAACAATACTACCCCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGcacca                                                                                         <  1:7432008/93‑1 (MQ=255)

cacCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGcacca                                                                                         <  1:4884283/93‑1 (MQ=255)

 cccTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGcaccat                                                                                        <  2:1729047/92‑1 (MQ=255)

  ccTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCaccatc                                                                                       <  2:1932894/93‑1 (MQ=255)

  ccTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCaccatc                                                                                       <  2:6169168/93‑1 (MQ=255)

    ttATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCgg                                                                                     >  2:1107421/1‑93 (MQ=255)

    ttATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCgg                                                                                     >  2:6031142/1‑93 (MQ=255)

     tATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGt                                                                                    >  1:2357786/1‑93 (MQ=255)

     tATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGt                                                                                    >  1:4199713/1‑93 (MQ=255)

       tCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCGCCTCTGACACAGCACCATCGGtaa                                                                                  <  1:4417447/93‑1 (MQ=255)

         cGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGtaata                                                                                <  1:5364231/93‑1 (MQ=255)

         cGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGCTTATCCACCTCTGACACAGCACCATCGGtaata                                                                                >  1:7619508/1‑93 (MQ=255)

          ggCATACAGAACAATACTGCACCGTAATTAATTTTACCCGCGTCTTCTGCATTGCCAGTTTATCCGCCTCTGACACAGCACCATCGGtaataa                                                                               <  2:5634043/93‑1 (MQ=255)

          ggCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGtaataa                                                                               >  2:3297349/1‑93 (MQ=255)

          ggCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGtaataa                                                                               >  1:2599579/1‑93 (MQ=255)

          ggCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGtaataa                                                                               >  1:5441616/1‑93 (MQ=255)

           gCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGtaataat                                                                              <  2:5741415/93‑1 (MQ=255)

            cATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGtaataata                                                                             <  1:2104029/93‑1 (MQ=255)

            cATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGtaataata                                                                             <  2:4491887/93‑1 (MQ=255)

              tACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTaataataat                                                                           >  1:3322240/1‑93 (MQ=255)

              tACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTaataataat                                                                           >  2:4572578/1‑93 (MQ=255)

               aCAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATg                                                                          >  1:563791/1‑93 (MQ=255)

               aCAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATg                                                                          <  2:1037568/93‑1 (MQ=255)

                 aGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTc                                                                        >  1:4178694/1‑93 (MQ=255)

                 aGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTc                                                                        <  2:2689569/93‑1 (MQ=255)

                  gAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCa                                                                       >  2:4999642/1‑93 (MQ=255)

                  gAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCa                                                                       >  2:7577546/1‑93 (MQ=255)

                  gAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCa                                                                       <  2:1400543/93‑1 (MQ=255)

                  gAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCa                                                                       >  2:7417488/1‑93 (MQ=255)

                  gAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCa                                                                       <  2:6783828/93‑1 (MQ=255)

                  gAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACACCACCATCGGTAATAATAATGTCa                                                                       >  2:1370180/1‑93 (MQ=255)

                      aaTACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAAttt                                                                   >  1:1593357/1‑93 (MQ=255)

                        tactacACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAAttttt                                                                 <  1:6243780/93‑1 (MQ=255)

                        tactacACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAAttttt                                                                 <  1:7805965/93‑1 (MQ=255)

                        tactacACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAAttttt                                                                 >  2:317632/1‑93 (MQ=255)

                           tacACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCg                                                              >  1:2863658/1‑93 (MQ=255)

                           tacACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCg                                                              >  2:1854386/1‑93 (MQ=255)

                            acacCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGa                                                             <  2:4199712/93‑1 (MQ=255)

                             cacCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGAt                                                            >  1:856722/1‑93 (MQ=255)

                               ccGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTg                                                          <  1:7566243/93‑1 (MQ=255)

                               ccGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTg                                                          >  2:3404899/1‑93 (MQ=255)

                               ccGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTg                                                          <  2:3599769/93‑1 (MQ=255)

                               ccGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTg                                                          >  1:3662705/1‑93 (MQ=255)

                                cGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTgg                                                         <  1:7886247/93‑1 (MQ=255)

                                cGTAATTAATTTTACCCGCGTCTTCTAAAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTgg                                                         <  2:7103567/93‑1 (MQ=255)

                                 gTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgt                                                        <  2:5892923/93‑1 (MQ=255)

                                 gTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgt                                                        >  2:7615982/1‑93 (MQ=255)

                                  taattaatTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgta                                                       <  1:5773079/93‑1 (MQ=255)

                                   aattaattTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgtag                                                      <  2:6499124/93‑1 (MQ=255)

                                    attaattTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgtagt                                                     <  1:1247447/93‑1 (MQ=255)

                                     ttaattTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgtagta                                                    <  1:1164682/93‑1 (MQ=255)

                                     ttaattTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgtagta                                                    >  1:3170813/1‑93 (MQ=255)

                                     ttaattTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgtagta                                                    >  1:5110925/1‑93 (MQ=255)

                                     ttaattTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgtagta                                                    <  2:5222988/93‑1 (MQ=255)

                                     ttaattTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGgtagta                                                    >  2:3206916/1‑93 (MQ=255)

                                      taattTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTAc                                                   <  2:4712636/93‑1 (MQ=255)

                                       aattTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTAcc                                                  <  2:2561824/93‑1 (MQ=255)

                                           ttACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGAt                                              <  1:4350199/93‑1 (MQ=255)

                                           tcaCCCACGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGAt                                              <  2:681472/91‑1 (MQ=255)

                                            tACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGAtt                                             <  2:7386440/93‑1 (MQ=255)

                                            tACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGAtt                                             >  2:719698/1‑93 (MQ=255)

                                             aCCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTa                                            >  2:7919837/1‑93 (MQ=255)

                                              cccGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTaa                                           >  2:407936/1‑93 (MQ=255)

                                                cgcgTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTaaaa                                         >  2:4333247/1‑93 (MQ=255)

                                                cgcgTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTaaaa                                         <  2:5524902/93‑1 (MQ=255)

                                                cgcgTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTaaaa                                         <  2:629882/93‑1 (MQ=255)

                                                 gcgTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAAc                                        <  1:6034137/93‑1 (MQ=255)

                                                 gcgTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAAc                                        >  2:1526924/1‑93 (MQ=255)

                                                    tcttctGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACcgc                                     >  1:2140950/1‑93 (MQ=255)

                                                    tcttctGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACcgc                                     <  1:1787257/93‑1 (MQ=255)

                                                           cAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGtt                              >  1:6989257/1‑93 (MQ=255)

                                                             aTGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTaa                            >  1:3399713/1‑93 (MQ=255)

                                                              tGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTaaa                           <  1:9526/93‑1 (MQ=255)

                                                              tGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTaaa                           >  2:3835840/1‑93 (MQ=255)

                                                              tGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTaaa                           >  1:1639756/1‑93 (MQ=255)

                                                                  aGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTc                       >  1:2303764/1‑93 (MQ=255)

                                                                  aGTTGATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTc                       <  2:6560811/93‑1 (MQ=255)

                                                                   gTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCg                      >  1:2655332/1‑93 (MQ=255)

                                                                   gTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCg                      >  1:2501653/1‑93 (MQ=255)

                                                                    tttATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGa                     >  1:4633298/1‑93 (MQ=255)

                                                                     ttATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGAt                    <  2:2153873/93‑1 (MQ=255)

                                                                       aTCCATCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGa                  <  2:2917060/93‑1 (MQ=255)

                                                                        tCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGaa                 <  1:4134839/93‑1 (MQ=255)

                                                                           aCCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCg              >  1:1890674/1‑93 (MQ=255)

                                                                             ctctGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCgag            <  1:3084171/93‑1 (MQ=255)

                                                                              tctGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCgaga           <  1:2201829/93‑1 (MQ=255)

                                                                              cctGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCgaga           <  1:364626/92‑1 (MQ=255)

                                                                              cctGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCgaga           <  1:5840995/92‑1 (MQ=255)

                                                                               ctGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGaa          <  1:7681468/93‑1 (MQ=255)

                                                                               ctGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGaa          <  2:5163570/93‑1 (MQ=255)

                                                                               ctGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGaa          >  2:3522544/1‑93 (MQ=255)

                                                                                tGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAAc         <  1:6618846/93‑1 (MQ=255)

                                                                                tGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAAc         <  2:7717425/93‑1 (MQ=255)

                                                                                  acacAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACgg       <  2:4626994/93‑1 (MQ=255)

                                                                                   cacaGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGc      >  1:6411923/1‑93 (MQ=255)

                                                                                    acaGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCa     >  2:6761030/1‑93 (MQ=255)

                                                                                       gCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCaa    >  1:6781250/1‑91 (MQ=255)

                                                                                       gCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATc  >  2:2603166/1‑93 (MQ=255)

                                                                                       gCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATc  >  2:2397528/1‑93 (MQ=255)

                                                                                       gCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATc  >  1:72202/1‑93 (MQ=255)

CACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATC  >  NC_000913/2167689‑2167868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATCACTT  >  NC_000913/2167686‑2167872

AAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCAC                                                                                               >  SRR4302543.3392595/1‑94 (MQ=60)

   CACCTTATCCGGCATACAGAACAATACTACCCCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCAT                                                                                            <  SRR4302543.7503258/94‑1 (MQ=60)

   CACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCAT                                                                                            >  SRR4302543.7229152/1‑94 (MQ=60)

   CACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCAT                                                                                            <  SRR4302543.4928812/94‑1 (MQ=60)

   CACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCAT                                                                                            <  SRR4302543.4928811/94‑1 (MQ=60)

   CACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCAT                                                                                            >  SRR4302543.4498305/1‑94 (MQ=60)

   CACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCAT                                                                                            >  SRR4302543.3680714/1‑94 (MQ=60)

    ACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATC                                                                                           <  SRR4302543.124457/94‑1 (MQ=60)

    ACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATC                                                                                           <  SRR4302543.3154897/94‑1 (MQ=60)

      CTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGT                                                                                         >  SRR4302543.1774064/1‑94 (MQ=60)

      CTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGG                                                                                         >  SRR4302543.4313390/1‑94 (MQ=60)

       TTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGT                                                                                        >  SRR4302543.2379167/1‑94 (MQ=60)

       TTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGT                                                                                        >  SRR4302543.4237790/1‑94 (MQ=60)

        TATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTA                                                                                       >  SRR4302543.2154566/1‑94 (MQ=60)

        TATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTA                                                                                       <  SRR4302543.1472007/94‑1 (MQ=60)

          TCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCGCCTCTGACACAGCACCATCGGTAAT                                                                                     <  SRR4302543.7909086/94‑1 (MQ=60)

          TCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCGCCTCTGACACAGCACCATCGGTAAT                                                                                     <  SRR4302543.4457489/94‑1 (MQ=60)

          TCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAAT                                                                                     <  SRR4302543.6017148/94‑1 (MQ=60)

           CCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGCTTATCCACCTCTGACACAGCACCATCGGTAATA                                                                                    >  SRR4302543.7691824/1‑94 (MQ=60)

            CGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAA                                                                                   <  SRR4302543.5412780/94‑1 (MQ=60)

            CGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAA                                                                                   >  SRR4302543.2623189/1‑94 (MQ=60)

            CGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAA                                                                                   >  SRR4302543.5491014/1‑94 (MQ=60)

             GGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAAT                                                                                  <  SRR4302543.4024005/94‑1 (MQ=60)

              GCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATA                                                                                 <  SRR4302543.4389899/94‑1 (MQ=60)

              GCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATA                                                                                 >  SRR4302543.4185769/1‑94 (MQ=60)

               CATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAA                                                                                <  SRR4302543.2122855/94‑1 (MQ=60)

                ATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAAT                                                                               <  SRR4302543.6850872/94‑1 (MQ=60)

                ATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAAT                                                                               >  SRR4302543.3352209/1‑94 (MQ=60)

                ATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAAT                                                                               >  SRR4302543.2412656/1‑94 (MQ=60)

                 TACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATG                                                                              >  SRR4302543.568958/1‑94 (MQ=60)

                  ACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGT                                                                             >  SRR4302543.886191/1‑94 (MQ=60)

                   CAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTC                                                                            >  SRR4302543.3575298/1‑94 (MQ=60)

                   CAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTC                                                                            >  SRR4302543.4216554/1‑94 (MQ=60)

                   CAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTC                                                                            >  SRR4302543.2961827/1‑94 (MQ=60)

                   CAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTC                                                                            >  SRR4302543.2532485/1‑94 (MQ=60)

                    AGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCA                                                                           <  SRR4302543.7335975/94‑1 (MQ=60)

                      AACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAAT                                                                         <  SRR4302543.3901977/94‑1 (MQ=60)

                      AACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAAT                                                                         <  SRR4302543.5395215/94‑1 (MQ=60)

                       ACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATT                                                                        <  SRR4302543.4218177/94‑1 (MQ=60)

                        CAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTT                                                                       <  SRR4302543.2437732/94‑1 (MQ=60)

                        CAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTT                                                                       >  SRR4302543.1607576/1‑94 (MQ=60)

                        CAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTT                                                                       >  SRR4302543.7382587/1‑94 (MQ=60)

                        CAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTT                                                                       >  SRR4302543.1977850/1‑94 (MQ=60)

                        CAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTT                                                                       >  SRR4302543.3298973/1‑94 (MQ=60)

                          ATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTT                                                                     >  SRR4302543.5844447/1‑94 (MQ=60)

                           TACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTT                                                                    <  SRR4302543.6302682/94‑1 (MQ=60)

                           TACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTT                                                                    <  SRR4302543.7879550/94‑1 (MQ=60)

                           TACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTT                                                                    >  SRR4302543.7647207/1‑94 (MQ=60)

                             CTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCG                                                                  <  SRR4302543.4218675/94‑1 (MQ=60)

                             CTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCG                                                                  >  SRR4302543.2889762/1‑94 (MQ=60)

                               ACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGAT                                                                >  SRR4302543.317603/1‑94 (MQ=60)

                               ACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGAT                                                                <  SRR4302543.2675530/94‑1 (MQ=60)

                               ACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGAT                                                                >  SRR4302543.864352/1‑94 (MQ=60)

                                CACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATGATAATGTCAATTTTTTCGATT                                                               >  SRR4302543.821534/1‑94 (MQ=60)

                                CACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATT                                                               <  SRR4302543.4830191/94‑1 (MQ=60)

                                 tctGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTG                                                              >  SRR4302543.2091619/4‑94 (MQ=60)

                                 ACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTG                                                              >  SRR4302543.3694870/1‑94 (MQ=60)

                                 ACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTG                                                              >  SRR4302543.4258157/1‑94 (MQ=60)

                                  CCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGG                                                             <  SRR4302543.1672866/94‑1 (MQ=60)

                                  CCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGG                                                             <  SRR4302543.7638242/94‑1 (MQ=60)

                                  CCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGG                                                             <  SRR4302543.593044/94‑1 (MQ=60)

                                  CCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGG                                                             <  SRR4302543.6898508/94‑1 (MQ=60)

                                   CGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGT                                                            <  SRR4302543.7960436/94‑1 (MQ=60)

                                     TAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAG                                                          <  SRR4302543.5825631/94‑1 (MQ=60)

                                     TAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAG                                                          <  SRR4302543.6262671/94‑1 (MQ=60)

                                     TAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAG                                                          >  SRR4302543.6416330/1‑94 (MQ=60)

                                      AATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGT                                                         <  SRR4302543.5730805/94‑1 (MQ=60)

                                       ATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTA                                                        <  SRR4302543.1258643/94‑1 (MQ=60)

                                       ATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTA                                                        >  SRR4302543.2841857/1‑94 (MQ=60)

                                       ATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTA                                                        >  SRR4302543.5157170/1‑94 (MQ=60)

                                       ATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTA                                                        >  SRR4302543.7955562/1‑94 (MQ=60)

                                       ATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTA                                                        >  SRR4302543.3199373/1‑94 (MQ=60)

                                        TTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTAC                                                       <  SRR4302543.1175278/94‑1 (MQ=60)

                                         TAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACC                                                      >  SRR4302543.5257437/1‑94 (MQ=60)

                                             TTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGAT                                                  <  SRR4302543.7742529/94‑1 (MQ=60)

                                              TTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATT                                                 <  SRR4302543.7754704/94‑1 (MQ=60)

                                              TTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATT                                                 <  SRR4302543.4389629/94‑1 (MQ=60)

                                                    GCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACC                                           <  SRR4302543.6090830/94‑1 (MQ=60)

                                                     CGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCG                                          >  SRR4302543.2595512/1‑94 (MQ=60)

                                                     CGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCG                                          >  SRR4302543.87283/1‑94 (MQ=60)

                                                      GTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGC                                         >  SRR4302543.5656950/1‑94 (MQ=60)

                                                      GTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGC                                         >  SRR4302543.2358718/1‑94 (MQ=60)

                                                      GTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGC                                         >  SRR4302543.2160110/1‑94 (MQ=60)

                                                       TCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCG                                        >  SRR4302543.6451887/1‑94 (MQ=60)

                                                       TCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCG                                        <  SRR4302543.1803244/94‑1 (MQ=60)

                                                        CTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGA                                       >  SRR4302543.212564/1‑94 (MQ=60)

                                                         TTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGAC                                      <  SRR4302543.2206614/94‑1 (MQ=60)

                                                         TTCTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGAC                                      <  SRR4302543.4994879/94‑1 (MQ=60)

                                                           CTGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGG                                    >  SRR4302543.2858376/1‑94 (MQ=60)

                                                            TGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGT                                   >  SRR4302543.4592140/1‑94 (MQ=60)

                                                            TGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGT                                   <  SRR4302543.5864481/94‑1 (MQ=60)

                                                            TGCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGT                                   >  SRR4302543.1620368/1‑94 (MQ=60)

                                                             GCAATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTT                                  >  SRR4302543.7055036/1‑94 (MQ=60)

                                                               AATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAA                                >  SRR4302543.3430172/1‑94 (MQ=60)

                                                                ATGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAA                               >  SRR4302543.1654384/1‑94 (MQ=60)

                                                                 TGCCAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAAT                              <  SRR4302543.9613/94‑1 (MQ=60)

                                                                    CAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTC                           <  SRR4302543.3394988/94‑1 (MQ=60)

                                                                    CAGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTC                           >  SRR4302543.2324750/1‑94 (MQ=60)

                                                                     AGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCG                          >  SRR4302543.2679407/1‑94 (MQ=60)

                                                                     AGTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCG                          >  SRR4302543.2524356/1‑94 (MQ=60)

                                                                      GTTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGA                         >  SRR4302543.4675423/1‑94 (MQ=60)

                                                                       TTTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGAT                        <  SRR4302543.1564167/94‑1 (MQ=60)

                                                                        TTATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATG                       <  SRR4302543.3337027/94‑1 (MQ=60)

                                                                          ATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAA                     >  SRR4302543.5114844/1‑94 (MQ=60)

                                                                          ATCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAA                     >  SRR4302543.568760/1‑94 (MQ=60)

                                                                           TCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAAT                    <  SRR4302543.4172154/94‑1 (MQ=60)

                                                                           TCCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAAT                    <  SRR4302543.783556/94‑1 (MQ=60)

                                                                            CCACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATC                   <  SRR4302543.680930/94‑1 (MQ=60)

                                                                             CACCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCG                  >  SRR4302543.1907674/1‑94 (MQ=60)

                                                                               CCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAG                <  SRR4302543.2303500/94‑1 (MQ=60)

                                                                               CCTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAG                <  SRR4302543.2278403/94‑1 (MQ=60)

                                                                                CTCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGA               <  SRR4302543.3111903/94‑1 (MQ=60)

                                                                                 TCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAA              <  SRR4302543.7565254/94‑1 (MQ=60)

                                                                                 TCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAA              <  SRR4302543.2221643/94‑1 (MQ=60)

                                                                                 TCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAA              >  SRR4302543.359581/1‑94 (MQ=60)

                                                                                 CCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAA              <  SRR4302543.5894133/94‑1 (MQ=60)

                                                                                 CCTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAA              <  SRR4302543.368005/94‑1 (MQ=60)

                                                                                  CTGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAAC             <  SRR4302543.7754230/94‑1 (MQ=60)

                                                                                   TGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACG            <  SRR4302543.6681259/94‑1 (MQ=60)

                                                                                   TGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACG            >  SRR4302543.5428915/1‑94 (MQ=60)

                                                                                   TGACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACG            <  SRR4302543.6681260/94‑1 (MQ=60)

                                                                                     ACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGC          >  SRR4302543.6472302/1‑94 (MQ=60)

                                                                                     ACACAGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGC          >  SRR4302543.1872869/1‑94 (MQ=60)

                                                                                         AGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATC      >  SRR4302543.72862/1‑94 (MQ=60)

                                                                                         AGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATA      >  SRR4302543.6845287/1‑94 (MQ=60)

                                                                                         AGCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATA      >  SRR4302543.6845286/1‑94 (MQ=60)

                                                                                          GCACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATCA     <  SRR4302543.7688281/94‑1 (MQ=60)

                                                                                           CACCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATCAC    <  SRR4302543.6561698/94‑1 (MQ=60)

                                                                                             CCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATCACTT  >  SRR4302543.277849/1‑94 (MQ=60)

AAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAACCGCGACGGTTAAATTTCGATGAATCGAGAACGGCAATCACTT  >  NC_000913/2167686‑2167872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`