Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,223,632 A→G L15P (CTG→CCG)  yohC ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,223,6320AG100.0% 374.4 / NA 121L15P (CTG→CCG) yohChypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (62/59);  total (62/59)

Only 100 of 127 total aligned reads displayed.

CCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT  >  NC_000913/2223543‑2223721
                                                                                         |                                                                                         
ccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTc                                                                                        >  1:4556325/1‑93 (MQ=255)
 cATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcc                                                                                       >  1:332396/1‑93 (MQ=255)
  aTCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTccc                                                                                      >  2:1227249/1‑93 (MQ=255)
   tCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcccc                                                                                     >  2:2398411/1‑93 (MQ=255)
   tCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcccc                                                                                     >  1:3996533/1‑93 (MQ=255)
    cAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCa                                                                                    <  1:3830110/93‑1 (MQ=255)
       cAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGaa                                                                                 >  1:4544110/1‑92 (MQ=255)
        aaaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACa                                                                                >  1:2362018/1‑93 (MQ=255)
        aaaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACa                                                                                >  2:594806/1‑93 (MQ=255)
        aaaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACa                                                                                <  1:145286/93‑1 (MQ=255)
        aaaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACa                                                                                >  2:3514420/1‑93 (MQ=255)
         aaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAGACGGGCCGCAGACAt                                                                               >  2:137888/1‑93 (MQ=255)
         aaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAt                                                                               >  1:4256275/1‑93 (MQ=255)
         aaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAt                                                                               >  2:2362574/1‑93 (MQ=255)
          aaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATg                                                                              >  1:1583445/1‑93 (MQ=255)
           aCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATgg                                                                             >  2:4369209/1‑93 (MQ=255)
            cGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGc                                                                            <  2:1726061/93‑1 (MQ=255)
            cGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGc                                                                            <  1:2990446/93‑1 (MQ=255)
             gtggtgGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCt                                                                           <  1:837557/93‑1 (MQ=255)
               ggtgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCa                                                                         >  1:3148400/1‑93 (MQ=255)
                gtgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCAt                                                                        >  1:730534/1‑93 (MQ=255)
                 tgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATa                                                                       <  1:3585160/93‑1 (MQ=255)
                 tgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATa                                                                       <  2:819037/93‑1 (MQ=255)
                 tgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATa                                                                       >  1:1009347/1‑93 (MQ=255)
                  gggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAc                                                                      <  1:1645594/93‑1 (MQ=255)
                  gggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAc                                                                      <  2:2386238/93‑1 (MQ=255)
                   ggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAcc                                                                     <  2:1316666/93‑1 (MQ=255)
                   ggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAcc                                                                     >  2:2821663/1‑93 (MQ=255)
                    gtgtAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCg                                                                    <  1:2527940/93‑1 (MQ=255)
                      gtAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGcc                                                                  <  2:3941149/93‑1 (MQ=255)
                      gtAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGcc                                                                  >  2:3096192/1‑93 (MQ=255)
                       tAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGGTCATACCGccc                                                                 >  2:173147/1‑93 (MQ=255)
                       tAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccc                                                                 <  1:1945770/93‑1 (MQ=255)
                         aTGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccccc                                                               >  2:3763061/1‑93 (MQ=255)
                          tGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCAGTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCt                                                              >  2:3828067/1‑93 (MQ=255)
                           ggTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCtt                                                             >  1:1726882/1‑93 (MQ=255)
                              gagaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCttgtt                                                          >  2:2643636/1‑93 (MQ=255)
                              gagaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCttgtt                                                          <  2:3548147/93‑1 (MQ=255)
                               agaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCttgttg                                                         >  1:1520722/1‑93 (MQ=255)
                                  aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      <  1:3269423/93‑1 (MQ=255)
                                  aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      <  2:468718/93‑1 (MQ=255)
                                  aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      <  1:4116317/93‑1 (MQ=255)
                                   aTCGTTTCGTTTTCGCGATTAATCCCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAg                                                     <  1:1590353/93‑1 (MQ=255)
                                   aTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAg                                                     <  1:1065446/93‑1 (MQ=255)
                                   aTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCACAGACATGGCTCATACCGCCCCCTTGTTGTGAg                                                     >  2:1459711/1‑93 (MQ=255)
                                    tcgtttcgttTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGt                                                    >  2:3337302/1‑93 (MQ=255)
                                     cgtttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGtt                                                   <  1:3569760/93‑1 (MQ=255)
                                      gtttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTc                                                  <  1:3555906/93‑1 (MQ=255)
                                      gtttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTc                                                  >  1:3662767/1‑93 (MQ=255)
                                       tttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCa                                                 >  2:2936533/1‑93 (MQ=255)
                                        ttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAt                                                >  2:4450287/1‑93 (MQ=255)
                                        ttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAt                                                >  1:372571/1‑93 (MQ=255)
                                        ttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAt                                                <  1:1894447/93‑1 (MQ=255)
                                        ttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAt                                                <  1:322400/93‑1 (MQ=255)
                                           gtttTCGCGATTAATCCCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGaa                                             <  1:2724417/93‑1 (MQ=255)
                                            ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            >  2:2629864/1‑93 (MQ=255)
                                            ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            >  1:1440430/1‑93 (MQ=255)
                                            ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            >  1:2492414/1‑93 (MQ=255)
                                            ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            >  1:4323602/1‑93 (MQ=255)
                                              ttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCt                                          <  2:3254130/93‑1 (MQ=255)
                                                 gcgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAc                                       <  1:2891095/93‑1 (MQ=255)
                                                  cgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACa                                      <  2:3594422/93‑1 (MQ=255)
                                                  cgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACa                                      <  2:2771886/93‑1 (MQ=255)
                                                   gATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAg                                     <  1:2938897/93‑1 (MQ=255)
                                                    aTTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGt                                    >  2:1012755/1‑93 (MQ=255)
                                                     ttAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGtt                                   >  1:119689/1‑93 (MQ=255)
                                                     ttAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGtt                                   >  1:2078327/1‑93 (MQ=255)
                                                     ttAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGtt                                   >  2:2915227/1‑93 (MQ=255)
                                                       aaTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCa                                 <  2:1863154/93‑1 (MQ=255)
                                                       aaTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCa                                 <  2:249482/93‑1 (MQ=255)
                                                        aTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCaa                                <  2:1595428/93‑1 (MQ=255)
                                                        aTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCACCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCaa                                >  2:4433721/1‑93 (MQ=255)
                                                         tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAg                               <  1:1031023/93‑1 (MQ=255)
                                                         tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAg                               <  1:1071849/93‑1 (MQ=255)
                                                           aCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGta                             <  1:1793475/93‑1 (MQ=255)
                                                           aCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGta                             >  2:3455499/1‑93 (MQ=255)
                                                             cTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGtata                           <  1:2063870/93‑1 (MQ=255)
                                                              tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGCACCTGACAGTTCAAGTATaa                          >  2:1694937/1‑93 (MQ=255)
                                                              tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATaa                          <  2:2639797/93‑1 (MQ=255)
                                                              tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATaa                          <  2:80434/93‑1 (MQ=255)
                                                              tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATaa                          <  1:4650760/93‑1 (MQ=255)
                                                                cATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGt                        >  2:429274/1‑93 (MQ=255)
                                                                 aTTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTc                       >  1:2034997/1‑93 (MQ=255)
                                                                  tttCACGATCGGGATGGGAGAACGGTCCCCAGTCATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCa                      <  2:4082128/93‑1 (MQ=255)
                                                                    tCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGc                    <  1:714259/93‑1 (MQ=255)
                                                                    tCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGc                    >  2:2640758/1‑93 (MQ=255)
                                                                    tCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGc                    >  1:2471237/1‑93 (MQ=255)
                                                                     cACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCt                   <  2:3860736/93‑1 (MQ=255)
                                                                      aCGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCtt                  >  2:540566/1‑93 (MQ=255)
                                                                       cGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtg                 >  2:866322/1‑93 (MQ=255)
                                                                       cGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtg                 <  1:4338600/93‑1 (MQ=255)
                                                                        gATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgt                <  1:3768562/93‑1 (MQ=255)
                                                                        gATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgt                <  2:3148344/93‑1 (MQ=255)
                                                                          tCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGa              <  1:987044/93‑1 (MQ=255)
                                                                           cGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTCAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGat             <  1:3298141/93‑1 (MQ=255)
                                                                              gATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGattat          >  2:4683815/1‑93 (MQ=255)
                                                                                  ggAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttttt      <  1:4236344/93‑1 (MQ=255)
                                                                                  ggAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttttt      <  1:4632953/93‑1 (MQ=255)
                                                                                   gAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAtttttt     >  1:127407/1‑93 (MQ=255)
                                                                                    aaaaCGGTCCCCAGACATGGCTCATAACGGCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGGATATGTCAGCTTGTGATTAtgttttt    >  2:3964730/1‑92 (MQ=255)
                                                                                         |                                                                                         
CCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT  >  NC_000913/2223543‑2223721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCG  >  NC_000913/2223540‑2223723
                                                                                            |                                                                                           
CCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGG                                                                                            >  SRR4302535.1279428/1‑94 (MQ=60)
  GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTC                                                                                          >  SRR4302535.4587440/1‑94 (MQ=60)
   CCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCC                                                                                         >  SRR4302535.334696/1‑94 (MQ=60)
    CATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCC                                                                                        >  SRR4302535.1739235/1‑94 (MQ=60)
     ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCC                                                                                       >  SRR4302535.4023876/1‑94 (MQ=60)
     ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCC                                                                                       >  SRR4302535.572099/1‑94 (MQ=60)
       CAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAG                                                                                     <  SRR4302535.3856527/94‑1 (MQ=60)
         GCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGAA                                                                                   >  SRR4302535.4575187/1‑94 (MQ=60)
          CAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACA                                                                                  >  SRR4302535.2378981/1‑94 (MQ=60)
           AAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAT                                                                                 >  SRR4302535.4285432/1‑94 (MQ=60)
           AAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAT                                                                                 <  SRR4302535.146299/94‑1 (MQ=60)
            AAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATG                                                                                >  SRR4302535.1594873/1‑94 (MQ=60)
             AACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGG                                                                               >  SRR4302535.1181193/1‑94 (MQ=60)
               CGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCT                                                                             <  SRR4302535.3011415/94‑1 (MQ=60)
                GTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTC                                                                            <  SRR4302535.843436/94‑1 (MQ=60)
                 TGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCA                                                                           >  SRR4302535.3170288/1‑94 (MQ=60)
                  GGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCAT                                                                          >  SRR4302535.735665/1‑94 (MQ=60)
                   GTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATA                                                                         >  SRR4302535.1016518/1‑94 (MQ=60)
                    TGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAC                                                                        <  SRR4302535.3610029/94‑1 (MQ=60)
                     GGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACC                                                                       <  SRR4302535.1657485/94‑1 (MQ=60)
                       GTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGC                                                                     <  SRR4302535.2546150/94‑1 (MQ=60)
                        TGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCC                                                                    >  SRR4302535.3600429/1‑94 (MQ=60)
                          TAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCC                                                                  <  SRR4302535.1959778/94‑1 (MQ=60)
                             TGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTT                                                               >  SRR4302535.1739367/1‑94 (MQ=60)
                                 GAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTG                                                           >  SRR4302535.1531676/1‑94 (MQ=60)
                                     AATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAG                                                       <  SRR4302535.3292135/94‑1 (MQ=60)
                                     AATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAG                                                       <  SRR4302535.4144492/94‑1 (MQ=60)
                                      ATCGTTTCGTTTTCGCGATTAATCCCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGT                                                      <  SRR4302535.1601828/94‑1 (MQ=60)
                                      ATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGT                                                      <  SRR4302535.1073026/94‑1 (MQ=60)
                                        CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTC                                                    >  SRR4302535.3688128/1‑94 (MQ=60)
                                        CGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTC                                                    <  SRR4302535.3594515/94‑1 (MQ=60)
                                        CGTGTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGAATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTC                                                    <  SRR4302535.2686913/94‑1 (MQ=60)
                                         GTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCA                                                   <  SRR4302535.3580569/94‑1 (MQ=60)
                                          TTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAT                                                  <  SRR4302535.3300188/94‑1 (MQ=60)
                                          TTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAT                                                  >  SRR4302535.375149/1‑94 (MQ=60)
                                           TTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATG                                                 <  SRR4302535.324631/94‑1 (MQ=60)
                                           TTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATG                                                 <  SRR4302535.1908083/94‑1 (MQ=60)
                                              GTTTTCGCGATTAATCCCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                              <  SRR4302535.2743874/94‑1 (MQ=60)
                                              GTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                              >  SRR4302535.2510400/1‑94 (MQ=60)
                                              GTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                              >  SRR4302535.4353177/1‑94 (MQ=60)
                                              GTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                              >  SRR4302535.1450731/1‑94 (MQ=60)
                                                TTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCT                                            >  SRR4302535.962896/1‑94 (MQ=60)
                                                   CGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAC                                         >  SRR4302535.3007683/1‑94 (MQ=60)
                                                    GCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACA                                        <  SRR4302535.2911548/94‑1 (MQ=60)
                                                      GATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGT                                      <  SRR4302535.2959568/94‑1 (MQ=60)
                                                       ATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTT                                     >  SRR4302535.2093300/1‑94 (MQ=60)
                                                       ATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTT                                     >  SRR4302535.120528/1‑94 (MQ=60)
                                                            TCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGT                                <  SRR4302535.1038344/94‑1 (MQ=60)
                                                            TCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGT                                <  SRR4302535.1079463/94‑1 (MQ=60)
                                                              ACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTAT                              <  SRR4302535.1806442/94‑1 (MQ=60)
                                                                CTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAA                            <  SRR4302535.2078749/94‑1 (MQ=60)
                                                                 TGCATTTCCCGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAG                           <  SRR4302535.191416/94‑1 (MQ=60)
                                                                 TGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAG                           <  SRR4302535.4682253/94‑1 (MQ=60)
                                                                   CATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTC                         <  SRR4302535.4229173/94‑1 (MQ=60)
                                                                   CATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTC                         >  SRR4302535.2049659/1‑94 (MQ=60)
                                                                      TTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGC                      >  SRR4302535.2489052/1‑94 (MQ=60)
                                                                       TCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCT                     <  SRR4302535.719275/94‑1 (MQ=60)
                                                                          CGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGT                  <  SRR4302535.4368269/94‑1 (MQ=60)
                                                                           GATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTG                 <  SRR4302535.3794576/94‑1 (MQ=60)
                                                                             TCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGAT               <  SRR4302535.994057/94‑1 (MQ=60)
                                                                              CGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATT              <  SRR4302535.3816724/94‑1 (MQ=60)
                                                                              CGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTCAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATT              <  SRR4302535.3321077/94‑1 (MQ=60)
                                                                               GGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTA             >  SRR4302535.4469645/1‑94 (MQ=60)
                                                                                   TGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTT         >  SRR4302535.1492451/1‑94 (MQ=60)
                                                                                     GGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTT       <  SRR4302535.4664384/94‑1 (MQ=60)
                                                                                     GGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTT       <  SRR4302535.4265337/94‑1 (MQ=60)
                                                                                     GGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTT       >  SRR4302535.128307/1‑94 (MQ=60)
                                                                                      GAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTG      <  SRR4302535.2112636/94‑1 (MQ=60)
                                                                                          ACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCG  >  SRR4302535.1773290/1‑94 (MQ=60)
                                                                                            |                                                                                           
CCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCG  >  NC_000913/2223540‑2223723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑