Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,223,632 A→G L15P (CTG→CCG)  yohC ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,223,6320AG99.6% 733.3 / ‑5.0 236L15P (CTG→CCG) yohChypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (119/116);  total (120/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.91e-01

Only 100 of 240 total aligned reads displayed.

GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT  >  NC_000913/2223542‑2223721
                                                                                          |                                                                                         
gccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGt                                                                                         >  1:7928312/1‑93 (MQ=255)
gccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGt                                                                                         <  2:2627304/93‑1 (MQ=255)
gccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGt                                                                                         >  1:6552002/1‑93 (MQ=255)
gccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGt                                                                                         <  1:1802796/93‑1 (MQ=255)
gccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGt                                                                                         >  1:6340527/1‑93 (MQ=255)
 ccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTc                                                                                        >  1:3122702/1‑93 (MQ=255)
 ccATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTc                                                                                        <  2:4445296/93‑1 (MQ=255)
  cATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcc                                                                                       >  1:5249534/1‑93 (MQ=255)
  cATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcc                                                                                       <  1:272684/93‑1 (MQ=255)
   aTCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTccc                                                                                      >  1:3096128/1‑93 (MQ=255)
    tCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcccc                                                                                     >  1:7249380/1‑93 (MQ=255)
    tCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTcccc                                                                                     >  1:7660306/1‑93 (MQ=255)
    tCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGCcccc                                                                                     >  1:5733998/1‑93 (MQ=255)
     cAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCa                                                                                    <  2:6592322/93‑1 (MQ=255)
     cAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCa                                                                                    <  1:1650863/93‑1 (MQ=255)
         aaagCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACa                                                                                <  1:4410095/93‑1 (MQ=255)
         aaaaCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACa                                                                                >  2:3373447/1‑93 (MQ=255)
             cGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGc                                                                            <  2:5579020/93‑1 (MQ=255)
              gtggtgGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGcc                                                                           >  1:2281447/1‑92 (MQ=255)
               tggtggGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTc                                                                          >  1:401498/1‑93 (MQ=255)
                ggtgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCa                                                                         <  2:7384063/93‑1 (MQ=255)
                 gtgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCAt                                                                        <  2:7628792/93‑1 (MQ=255)
                  tgggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATa                                                                       <  1:7825905/93‑1 (MQ=255)
                  tgggtgTAATGGTGAGAAATCGTTTAGTTTTCGTGATTAATCACTTTCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATa                                                                       <  2:6802599/93‑1 (MQ=255)
                   gggtgTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAc                                                                      >  2:3363000/1‑93 (MQ=255)
                     gtgtAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCAtcacg                                                                    >  1:2398047/1‑89 (MQ=255)
                      tgtAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGc                                                                   <  2:7004993/93‑1 (MQ=255)
                       gtAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGcc                                                                  >  2:831112/1‑93 (MQ=255)
                        tAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccc                                                                 >  1:3473292/1‑93 (MQ=255)
                        tAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGccc                                                                 >  1:7437735/1‑93 (MQ=255)
                           tGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAACGCCCCCt                                                              >  1:4761989/1‑93 (MQ=255)
                             gTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCttg                                                            >  1:5943524/1‑93 (MQ=255)
                               gagaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCttgtt                                                          >  2:5975615/1‑93 (MQ=255)
                                 gaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTtgttgt                                                        <  2:489234/93‑1 (MQ=255)
                                 gaAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTtgttgt                                                        >  2:2438694/1‑93 (MQ=255)
                                   aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      <  1:2012547/93‑1 (MQ=255)
                                   aaTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGa                                                      >  1:1850596/1‑93 (MQ=255)
                                    aTCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAg                                                     <  2:6633535/93‑1 (MQ=255)
                                     tcgtttcgttTTCGCGATTAATCACCTGCATTTCACGATCGGCATGGAAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGt                                                    <  2:3503408/93‑1 (MQ=255)
                                       gtttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTc                                                  <  1:1225483/93‑1 (MQ=255)
                                        tttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCa                                                 >  2:6089711/1‑93 (MQ=255)
                                        tttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCa                                                 >  2:2767803/1‑93 (MQ=255)
                                         ttcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAt                                                >  2:6740484/1‑93 (MQ=255)
                                          tcgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATg                                               <  1:3573431/93‑1 (MQ=255)
                                           cgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGa                                              <  2:1870544/93‑1 (MQ=255)
                                           cgtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGa                                              <  1:4246135/93‑1 (MQ=255)
                                            gtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGaa                                             <  2:1558443/93‑1 (MQ=255)
                                            gtttTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGaa                                             <  2:3772636/93‑1 (MQ=255)
                                             ttttCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            >  2:500882/1‑93 (MQ=255)
                                             ttttCGAGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAc                                            <  1:5589207/93‑1 (MQ=255)
                                                  gcgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAc                                       >  2:2579139/1‑93 (MQ=255)
                                                   cgATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACa                                      <  2:367426/93‑1 (MQ=255)
                                                    gATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAg                                     <  1:4968804/93‑1 (MQ=255)
                                                    gATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGGCCCCAGACATGGCTCATACCGGCCCCTTGTTGTGAGTTCATGAACCTGACAg                                     >  2:4252733/1‑93 (MQ=255)
                                                     aTTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGt                                    >  1:7160851/1‑93 (MQ=255)
                                                        aaTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCa                                 >  1:5269392/1‑93 (MQ=255)
                                                        aaTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCa                                 >  1:2849798/1‑93 (MQ=255)
                                                        aaTCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCa                                 <  1:6656072/93‑1 (MQ=255)
                                                          tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAg                               >  2:4821291/1‑93 (MQ=255)
                                                          tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAg                               >  1:5104129/1‑93 (MQ=255)
                                                          tCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAg                               >  2:5747851/1‑93 (MQ=255)
                                                            cccTGCATTTCACGATCGGTATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGta                             <  1:2190234/92‑1 (MQ=255)
                                                            aCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGta                             >  1:4301125/1‑93 (MQ=255)
                                                            aCCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGta                             >  1:26627/1‑93 (MQ=255)
                                                              cTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGtata                           >  2:7867863/1‑93 (MQ=255)
                                                              cTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGtata                           <  2:2850428/93‑1 (MQ=255)
                                                              cTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGtata                           >  1:2919472/1‑93 (MQ=255)
                                                               tGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATaa                          <  1:7149118/93‑1 (MQ=255)
                                                                gCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATCCCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAg                         <  2:3075414/93‑1 (MQ=255)
                                                                gCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAg                         <  1:5447560/93‑1 (MQ=255)
                                                                gCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAg                         >  2:1665306/1‑93 (MQ=255)
                                                                gCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAg                         >  1:661221/1‑93 (MQ=255)
                                                                  aTTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTc                       <  2:4710708/93‑1 (MQ=255)
                                                                   tttCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCa                      >  2:630207/1‑93 (MQ=255)
                                                                    ttCACGATCTGGATGGGAAAACGGTCCCCAGACCTGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAg                     <  1:1369750/93‑1 (MQ=255)
                                                                     tCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGc                    >  1:1028686/1‑93 (MQ=255)
                                                                      cACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCt                   >  1:6079808/1‑93 (MQ=255)
                                                                      cACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCt                   >  2:5314766/1‑93 (MQ=255)
                                                                       aCGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCtt                  >  2:1399711/1‑93 (MQ=255)
                                                                          aTCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTtgtg               >  1:5480540/1‑93 (MQ=255)
                                                                           tCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGa              >  1:4574639/1‑93 (MQ=255)
                                                                            cGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGat             <  2:2189459/93‑1 (MQ=255)
                                                                            cGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGat             <  2:7310652/93‑1 (MQ=255)
                                                                            cGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGat             <  2:6918618/93‑1 (MQ=255)
                                                                             gggATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGatt            >  2:272590/1‑93 (MQ=255)
                                                                               gATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGattat          >  2:6504342/1‑93 (MQ=255)
                                                                               gATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGattat          >  2:7546941/1‑93 (MQ=255)
                                                                               gATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTCCAAGTATAAGTCAGCTTGTGattat          >  1:880928/1‑93 (MQ=255)
                                                                                aTGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGattatt         >  2:1185169/1‑93 (MQ=255)
                                                                                 tGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttt        <  1:4663713/93‑1 (MQ=255)
                                                                                 tGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAttt        <  1:1435925/93‑1 (MQ=255)
                                                                                    gAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAtttttt     <  2:7526537/93‑1 (MQ=255)
                                                                                    gAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTAtttttt     <  2:3984466/93‑1 (MQ=255)
                                                                                     aaaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTg    <  1:6898549/93‑1 (MQ=255)
                                                                                     aaaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTg    <  1:6851478/93‑1 (MQ=255)
                                                                                     aaaaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTg    <  1:3793632/93‑1 (MQ=255)
                                                                                      aaaCGGGCCCCAGGCATGGCCCATTCCGGCCCCTTGGTTTGAGTTTATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGt   <  2:67047/93‑1 (MQ=255)
                                                                                       aaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGtt  >  2:131084/1‑93 (MQ=255)
                                                                                       aaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGtt  >  1:6444541/1‑93 (MQ=255)
                                                                                       aaCGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGtt  >  1:3040572/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT  >  NC_000913/2223542‑2223721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGC  >  NC_000913/2223539‑2223724
                                                                                             |                                                                                            
GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACG                                                                                              >  SRR4302543.4438738/1‑94 (MQ=60)
 CCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGG                                                                                             >  SRR4302543.3336212/1‑94 (MQ=60)
  CGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGT                                                                                            >  SRR4302543.8002755/1‑94 (MQ=60)
  CGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGT                                                                                            >  SRR4302543.6400146/1‑94 (MQ=60)
  CGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGT                                                                                            >  SRR4302543.2368498/1‑94 (MQ=60)
  CGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGT                                                                                            >  SRR4302543.6613669/1‑94 (MQ=60)
  CGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGT                                                                                            >  SRR4302543.7002835/1‑94 (MQ=60)
   GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTC                                                                                           >  SRR4302543.3150788/1‑94 (MQ=60)
   GCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTC                                                                                           <  SRR4302543.1818930/94‑1 (MQ=60)
    CCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCC                                                                                          >  SRR4302543.5296857/1‑94 (MQ=60)
     CATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCC                                                                                         <  SRR4302543.275081/94‑1 (MQ=60)
     CATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCC                                                                                         >  SRR4302543.3123977/1‑94 (MQ=60)
      ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCC                                                                                        >  SRR4302543.6494261/1‑94 (MQ=60)
      ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCC                                                                                        >  SRR4302543.7732914/1‑94 (MQ=60)
      ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCC                                                                                        >  SRR4302543.7732915/1‑94 (MQ=60)
      ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCC                                                                                        >  SRR4302543.7319497/1‑94 (MQ=60)
      ATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGCCCCC                                                                                        >  SRR4302543.5786311/1‑94 (MQ=60)
        CAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAG                                                                                      <  SRR4302543.1665631/94‑1 (MQ=60)
        CAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAG                                                                                      >  SRR4302543.4588142/1‑94 (MQ=60)
           CAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACA                                                                                   >  SRR4302543.144984/1‑94 (MQ=60)
            AAAGCGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAT                                                                                  <  SRR4302543.4450068/94‑1 (MQ=60)
            AAAACGTGGTTGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACAT                                                                                  <  SRR4302543.5825567/94‑1 (MQ=60)
             AAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATG                                                                                 >  SRR4302543.6545359/1‑94 (MQ=60)
              AACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGG                                                                                >  SRR4302543.6949493/1‑94 (MQ=60)
                CGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCC                                                                              >  SRR4302543.2302298/1‑94 (MQ=60)
                 GTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTC                                                                             >  SRR4302543.405205/1‑94 (MQ=60)
                   GGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCAT                                                                           <  SRR4302543.1843820/94‑1 (MQ=60)
                    GTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATA                                                                          >  SRR4302543.7094055/1‑94 (MQ=60)
                     TGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAC                                                                         <  SRR4302543.1438851/94‑1 (MQ=60)
                     TGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAC                                                                         <  SRR4302543.7899614/94‑1 (MQ=60)
                      GGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACC                                                                        >  SRR4302543.1769498/1‑94 (MQ=60)
                      GGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACC                                                                        <  SRR4302543.658356/94‑1 (MQ=60)
                       GGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATcacg                                                                       >  SRR4302543.2419738/1‑90 (MQ=60)
                       GGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCG                                                                       <  SRR4302543.1275219/94‑1 (MQ=60)
                         TGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCC                                                                     >  SRR4302543.5609993/1‑94 (MQ=60)
                         TGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCC                                                                     >  SRR4302543.3130893/1‑94 (MQ=60)
                          GTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCC                                                                    >  SRR4302543.3504403/1‑94 (MQ=60)
                          GTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCC                                                                    >  SRR4302543.7509031/1‑94 (MQ=60)
                           TAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCC                                                                   >  SRR4302543.4010831/1‑94 (MQ=60)
                            AATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCC                                                                  >  SRR4302543.4258160/1‑94 (MQ=60)
                             ATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATAACGCCCCCT                                                                 >  SRR4302543.4805501/1‑94 (MQ=60)
                              TGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTT                                                                <  SRR4302543.2804657/94‑1 (MQ=60)
                               GGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTG                                                               <  SRR4302543.4792702/94‑1 (MQ=60)
                               GGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTG                                                               >  SRR4302543.5997915/1‑94 (MQ=60)
                                GTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGT                                                              >  SRR4302543.747515/1‑94 (MQ=60)
                                   AGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGT                                                           >  SRR4302543.4633444/1‑94 (MQ=60)
                                     AAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGA                                                         >  SRR4302543.4842022/1‑94 (MQ=60)
                                     AAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGA                                                         >  SRR4302543.2398554/1‑94 (MQ=60)
                                     AAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGA                                                         >  SRR4302543.1867243/1‑94 (MQ=60)
                                      AATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAG                                                        >  SRR4302543.4319334/1‑94 (MQ=60)
                                      AATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAG                                                        <  SRR4302543.2030637/94‑1 (MQ=60)
                                      AATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAG                                                        >  SRR4302543.4319333/1‑94 (MQ=60)
                                       ATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGT                                                       >  SRR4302543.2950154/1‑94 (MQ=60)
                                          GTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCA                                                    >  SRR4302543.2912867/1‑94 (MQ=60)
                                          GTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCA                                                    <  SRR4302543.1236496/94‑1 (MQ=60)
                                          GTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCA                                                    >  SRR4302543.6225174/1‑94 (MQ=60)
                                           TTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAT                                                   >  SRR4302543.4353732/1‑94 (MQ=60)
                                           TTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAT                                                   <  SRR4302543.531630/94‑1 (MQ=60)
                                           TTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCAT                                                   <  SRR4302543.526263/94‑1 (MQ=60)
                                             TCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGA                                                 >  SRR4302543.5324868/1‑94 (MQ=60)
                                             TCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGA                                                 <  SRR4302543.3605018/94‑1 (MQ=60)
                                              CGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                <  SRR4302543.1151077/94‑1 (MQ=60)
                                              CGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                >  SRR4302543.6618222/1‑94 (MQ=60)
                                              CGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                <  SRR4302543.4284666/94‑1 (MQ=60)
                                              CGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAA                                                >  SRR4302543.2725638/1‑94 (MQ=60)
                                               GTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAAC                                               <  SRR4302543.3638123/94‑1 (MQ=60)
                                                TTTTCGAGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACC                                              <  SRR4302543.5640121/94‑1 (MQ=60)
                                                  TTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTG                                            <  SRR4302543.6130919/94‑1 (MQ=60)
                                                   TCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGA                                           <  SRR4302543.4834066/94‑1 (MQ=60)
                                                    CGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAC                                          >  SRR4302543.7968273/1‑94 (MQ=60)
                                                    CGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGAC                                          >  SRR4302543.7790977/1‑94 (MQ=60)
                                                     GCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACA                                         <  SRR4302543.2174605/94‑1 (MQ=60)
                                                      CGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAG                                        >  SRR4302543.6181650/1‑94 (MQ=60)
                                                       GATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGT                                       >  SRR4302543.7230425/1‑94 (MQ=60)
                                                       GATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGT                                       <  SRR4302543.5014028/94‑1 (MQ=60)
                                                       GATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGT                                       <  SRR4302543.3161594/94‑1 (MQ=60)
                                                       CATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGT                                       <  SRR4302543.2252392/94‑1 (MQ=60)
                                                        ATTAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTT                                      <  SRR4302543.2440398/94‑1 (MQ=60)
                                                          TAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCA                                    >  SRR4302543.2875814/1‑94 (MQ=60)
                                                          TAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCA                                    >  SRR4302543.5316934/1‑94 (MQ=60)
                                                          TAATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCA                                    <  SRR4302543.1984452/94‑1 (MQ=60)
                                                           AATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAA                                   <  SRR4302543.6718915/94‑1 (MQ=60)
                                                            ATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAG                                  >  SRR4302543.5150324/1‑94 (MQ=60)
                                                            ATCACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAG                                  <  SRR4302543.5716482/94‑1 (MQ=60)
                                                              CACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTA                                >  SRR4302543.26867/1‑94 (MQ=60)
                                                              CACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTA                                >  SRR4302543.6817580/1‑94 (MQ=60)
                                                              CACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTA                                >  SRR4302543.4340201/1‑94 (MQ=60)
                                                              CACCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTA                                <  SRR4302543.5504378/94‑1 (MQ=60)
                                                               CCCTGCATTTCACGATCGGTATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTAT                               <  SRR4302543.2209901/94‑1 (MQ=60)
                                                                CCTGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATA                              >  SRR4302543.2946003/1‑94 (MQ=60)
                                                                  TGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAG                            <  SRR4302543.5768356/94‑1 (MQ=60)
                                                                  TGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAG                            >  SRR4302543.667297/1‑94 (MQ=60)
                                                                  TGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAG                            <  SRR4302543.7218608/94‑1 (MQ=60)
                                                                  TGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAG                            <  SRR4302543.7218607/94‑1 (MQ=60)
                                                                  TGCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAG                            <  SRR4302543.3118537/94‑1 (MQ=60)
                                                                   GCATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGT                           <  SRR4302543.5496987/94‑1 (MQ=60)
                                                                    CATTTCACGATCGGGATGGGACAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGCTCAAGTATAAGTC                          >  SRR4302543.802966/1‑94 (MQ=60)
                                                                    CATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTC                          <  SRR4302543.3574197/94‑1 (MQ=60)
                                                                     ATTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCA                         >  SRR4302543.6702257/1‑94 (MQ=60)
                                                                      TTTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAG                        <  SRR4302543.1761486/94‑1 (MQ=60)
                                                                       TTCACGATCTGGATGGGAAAACGGTCCCCAGACCTGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGC                       <  SRR4302543.1382047/94‑1 (MQ=60)
                                                                       TTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGC                       >  SRR4302543.1037801/1‑94 (MQ=60)
                                                                       TTCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGC                       <  SRR4302543.1382048/94‑1 (MQ=60)
                                                                        TCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCT                      <  SRR4302543.898420/94‑1 (MQ=60)
                                                                        TCACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCT                      >  SRR4302543.6137425/1‑94 (MQ=60)
                                                                         CACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTT                     <  SRR4302543.5268649/94‑1 (MQ=60)
                                                                          ACGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTG                    <  SRR4302543.3799328/94‑1 (MQ=60)
                                                                           CGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGT                   >  SRR4302543.4592354/1‑94 (MQ=60)
                                                                           CGATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGC                   >  SRR4302543.7316132/1‑94 (MQ=60)
                                                                            GATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTG                  >  SRR4302543.5530211/1‑94 (MQ=60)
                                                                             ATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATCCCGACCCCTTGTTGTGAGATCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGT                 >  SRR4302543.281814/1‑94 (MQ=60)
                                                                             ATCGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGA                 >  SRR4302543.4616234/1‑94 (MQ=60)
                                                                               CGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATT               >  SRR4302543.5508553/1‑94 (MQ=60)
                                                                               CGGGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATT               <  SRR4302543.2952305/94‑1 (MQ=60)
                                                                                 GGATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTCCAAGTATAAGTCAGCTTGTGATTAT             >  SRR4302543.888746/1‑94 (MQ=60)
                                                                                   ATGGTAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTT           <  SRR4302543.7780206/94‑1 (MQ=60)
                                                                                   ATGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTT           <  SRR4302543.211568/94‑1 (MQ=60)
                                                                                    TGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTT          <  SRR4302543.1448905/94‑1 (MQ=60)
                                                                                    TGGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTT          <  SRR4302543.4706212/94‑1 (MQ=60)
                                                                                     GGGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTT         <  SRR4302543.744860/94‑1 (MQ=60)
                                                                                      GGAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTT        <  SRR4302543.3315755/94‑1 (MQ=60)
                                                                                       GAAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTG       <  SRR4302543.5020037/94‑1 (MQ=60)
                                                                                        AAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGT      <  SRR4302543.3827057/94‑1 (MQ=60)
                                                                                        AAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGT      <  SRR4302543.6963380/94‑1 (MQ=60)
                                                                                        AAAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGT      <  SRR4302543.6916100/94‑1 (MQ=60)
                                                                                         AAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT     >  SRR4302543.3067992/1‑94 (MQ=60)
                                                                                         AAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT     <  SRR4302543.68870/94‑1 (MQ=60)
                                                                                         AAACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTT     >  SRR4302543.6505254/1‑94 (MQ=60)
                                                                                          AACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTG    >  SRR4302543.6010662/1‑94 (MQ=60)
                                                                                          AACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTC    >  SRR4302543.2043747/1‑94 (MQ=60)
                                                                                           ACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCG   <  SRR4302543.342359/94‑1 (MQ=60)
                                                                                           ACGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCG   <  SRR4302543.7889749/94‑1 (MQ=60)
                                                                                            CGGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGC  >  SRR4302543.6777229/1‑94 (MQ=60)
                                                                                             |                                                                                            
GCCGCCATCAGCAAAACGTGGTGGGTGTAATGGTGAGAAATCGTTTCGTTTTCGCGATTAATCACCTGCATTTCACGATCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGC  >  NC_000913/2223539‑2223724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑