Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,225,570 T→C T324A (ACA→GCA)  yohG ← putative channel/filament proteins

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,225,5700TC100.0% 490.1 / NA 153T324A (ACA→GCA) yohGputative channel/filament proteins
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (64/89);  total (64/89)

Only 100 of 159 total aligned reads displayed.

TGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTT  >  NC_000913/2225480‑2225659
                                                                                          |                                                                                         
 gCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTg                                                                                        <  2:230316/93‑1 (MQ=255)
  cccGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGa                                                                                       >  1:3443385/1‑93 (MQ=255)
   ccGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGaa                                                                                      <  1:2596327/93‑1 (MQ=255)
      cgTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCt                                                                                   <  2:1122538/93‑1 (MQ=255)
       gTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTg                                                                                  <  2:1598332/93‑1 (MQ=255)
         tAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGAc                                                                                <  2:3209555/93‑1 (MQ=255)
          aaaGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACt                                                                               <  1:1259103/93‑1 (MQ=255)
           aaGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTg                                                                              <  2:541483/93‑1 (MQ=255)
             acgcgcgTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGc                                                                            <  2:3942846/92‑1 (MQ=255)
                 gcCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGcc                                                                        >  2:3456776/1‑93 (MQ=255)
                  ccTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGccc                                                                       <  1:304532/93‑1 (MQ=255)
                    tGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCcgc                                                                     >  1:3731266/1‑93 (MQ=255)
                       gcAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGcc                                                                  <  2:2364670/93‑1 (MQ=255)
                       gcAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGcc                                                                  >  1:3973202/1‑93 (MQ=255)
                          aGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACg                                                               >  1:3694710/1‑93 (MQ=255)
                           gACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGt                                                              <  1:522199/93‑1 (MQ=255)
                           gACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGt                                                              <  2:2620984/93‑1 (MQ=255)
                            aCCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTc                                                             <  1:825416/93‑1 (MQ=255)
                            aCCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTc                                                             >  2:3025222/1‑93 (MQ=255)
                            aCCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTc                                                             <  2:2598571/93‑1 (MQ=255)
                             ccTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCa                                                            >  1:4398299/1‑93 (MQ=255)
                              cTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAt                                                           <  1:1176085/93‑1 (MQ=255)
                              cTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAt                                                           <  2:4255053/93‑1 (MQ=255)
                                 ccACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCa                                                        <  1:30202/93‑1 (MQ=255)
                                 ccACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCa                                                        <  2:451207/93‑1 (MQ=255)
                                  cacaCGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCAc                                                       >  2:27310/1‑93 (MQ=255)
                                  cacaCGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCAc                                                       <  1:1995076/93‑1 (MQ=255)
                                   acacGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCAcc                                                      <  2:413059/93‑1 (MQ=255)
                                     acGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGc                                                    >  2:4679952/1‑93 (MQ=255)
                                     acGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGc                                                    <  1:3556670/93‑1 (MQ=255)
                                       gCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCtt                                                  >  1:3351304/1‑93 (MQ=255)
                                       gCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCtt                                                  >  2:4302466/1‑93 (MQ=255)
                                        cAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTc                                                 >  1:2788953/1‑93 (MQ=255)
                                        cAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTc                                                 <  1:4166583/93‑1 (MQ=255)
                                         aaGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCa                                                >  2:2554149/1‑93 (MQ=255)
                                         aaGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCa                                                <  1:2672049/93‑1 (MQ=255)
                                          aGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCaa                                               >  2:659738/1‑93 (MQ=255)
                                           ggCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAac                                              <  2:3133170/93‑1 (MQ=255)
                                           ggCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAac                                              <  2:4019714/93‑1 (MQ=255)
                                               tCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAaccacc                                          <  2:2962861/93‑1 (MQ=255)
                                               tCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAaccacc                                          >  1:2694550/1‑93 (MQ=255)
                                                 gcgcTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGc                                        <  2:952899/93‑1 (MQ=255)
                                                 gcgcTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGc                                        <  1:384326/93‑1 (MQ=255)
                                                   gcTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCtt                                      >  2:597065/1‑93 (MQ=255)
                                                   gcTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCtt                                      >  1:1512948/1‑93 (MQ=255)
                                                   gcTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCtt                                      <  2:3582620/93‑1 (MQ=255)
                                                    cTCAATTTGCGCGTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCttt                                     <  2:1817924/93‑1 (MQ=255)
                                                     tCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCTCCACGTCATTCACCGCTTCAACCACCGCTttg                                    <  2:4147565/93‑1 (MQ=255)
                                                        aTTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTttgttg                                 >  1:2980812/1‑93 (MQ=255)
                                                        aTTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTttgttg                                 <  1:2171074/93‑1 (MQ=255)
                                                         tttGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTtgttgt                                >  1:893786/1‑93 (MQ=255)
                                                         tttGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTtgttgt                                <  1:1589389/93‑1 (MQ=255)
                                                          ttGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTa                               <  2:1912179/93‑1 (MQ=255)
                                                             cgcCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCt                            <  2:2601031/93‑1 (MQ=255)
                                                             cgcCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCt                            <  2:356259/93‑1 (MQ=255)
                                                              gcCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTg                           <  1:1520392/93‑1 (MQ=255)
                                                              gcCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTg                           >  1:4041207/1‑93 (MQ=255)
                                                              cctggATGTTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTg                           <  2:801623/89‑1 (MQ=255)
                                                                cTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGc                         >  2:107292/1‑93 (MQ=255)
                                                                cTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGc                         >  1:1388228/1‑93 (MQ=255)
                                                                cTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGc                         >  1:1362505/1‑93 (MQ=255)
                                                                 tGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTCTGTTGTAGCTGGCg                        >  2:1905433/1‑93 (MQ=255)
                                                                  gATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGa                       >  2:1241886/1‑93 (MQ=255)
                                                                    tGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATa                     >  1:1555140/1‑93 (MQ=255)
                                                                    tGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATa                     >  1:2475072/1‑93 (MQ=255)
                                                                    tGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATa                     <  1:499995/93‑1 (MQ=255)
                                                                    tGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATa                     <  1:3328952/93‑1 (MQ=255)
                                                                    tGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATa                     >  2:2512694/1‑93 (MQ=255)
                                                                    tGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATa                     <  1:98616/93‑1 (MQ=255)
                                                                    tGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATa                     >  2:2051787/1‑93 (MQ=255)
                                                                      cTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGa                   <  1:3824285/93‑1 (MQ=255)
                                                                      cTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGa                   >  1:604452/1‑93 (MQ=255)
                                                                      cTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGa                   <  2:2170570/93‑1 (MQ=255)
                                                                       tGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGAc                  >  2:242468/1‑93 (MQ=255)
                                                                       tGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGAc                  >  2:1437110/1‑93 (MQ=255)
                                                                         gTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACaa                >  1:4208642/1‑93 (MQ=255)
                                                                         gTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACaa                >  2:398581/1‑93 (MQ=255)
                                                                           ttttCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGt              <  1:358087/93‑1 (MQ=255)
                                                                           ttttCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGt              <  1:4278215/93‑1 (MQ=255)
                                                                           ttttCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGt              <  2:3887378/93‑1 (MQ=255)
                                                                           ttttCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGt              >  2:3831908/1‑93 (MQ=255)
                                                                           ttttCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGt              >  2:3536293/1‑93 (MQ=255)
                                                                           ttttCTCCGCCACTGCCTGACCCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGt              <  2:2003895/93‑1 (MQ=255)
                                                                            tgtctcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGtt             <  1:1711943/91‑1 (MQ=255)
                                                                             ttCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTg            >  1:3168645/1‑93 (MQ=255)
                                                                              tctcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGc           <  1:2075042/93‑1 (MQ=255)
                                                                               ctcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCt          <  1:4508643/93‑1 (MQ=255)
                                                                                tcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCtt         <  1:3799410/93‑1 (MQ=255)
                                                                                 ccGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCttt        <  2:2101432/93‑1 (MQ=255)
                                                                                  cGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTc       <  2:3576607/93‑1 (MQ=255)
                                                                                  cGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTc       <  2:167623/93‑1 (MQ=255)
                                                                                  cGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTc       <  2:1959914/93‑1 (MQ=255)
                                                                                   gCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCg      >  1:635018/1‑93 (MQ=255)
                                                                                   gCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGCTGCTTTCg      >  1:1230958/1‑93 (MQ=255)
                                                                                    ccAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCgg     >  1:1888456/1‑93 (MQ=255)
                                                                                     cAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGc    >  1:545655/1‑93 (MQ=255)
                                                                                      aGTGCCTGAACCTGACTGGCTGCCCGCGCCCCGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGCCAAGTTGCTTTCGGCt   >  1:3166853/1‑93 (MQ=255)
                                                                                      aGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCt   <  2:3407099/93‑1 (MQ=255)
                                                                                       gtgcCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCtt  <  1:1504696/93‑1 (MQ=255)
                                                                                       gtgcCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCtt  <  1:1128747/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTT  >  NC_000913/2225480‑2225659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTGC  >  NC_000913/2225479‑2225663
                                                                                           |                                                                                             
ATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCT                                                                                             <  SRR4302535.3449037/94‑1 (MQ=60)
ATGCACGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCT                                                                                             <  SRR4302535.4196548/94‑1 (MQ=60)
  GCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGA                                                                                           >  SRR4302535.3467332/1‑94 (MQ=60)
    CCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAAC                                                                                         <  SRR4302535.2614974/94‑1 (MQ=60)
          TAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACT                                                                                   >  SRR4302535.563727/1‑94 (MQ=60)
           AAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTG                                                                                  <  SRR4302535.1268114/94‑1 (MQ=60)
             AGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGC                                                                                >  SRR4302535.325573/1‑94 (MQ=60)
              GCGCGCCTCCGCAAGACCTCCCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGGCTGGCT                                                                               <  SRR4302535.3071151/94‑1 (MQ=60)
                 CGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCC                                                                            <  SRR4302535.795793/94‑1 (MQ=60)
                  GCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCC                                                                           >  SRR4302535.3831491/1‑94 (MQ=60)
                   CCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCG                                                                          <  SRR4302535.306645/94‑1 (MQ=60)
                    CTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGC                                                                         >  SRR4302535.3757103/1‑94 (MQ=60)
                       CGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCC                                                                      >  SRR4302535.4000342/1‑94 (MQ=60)
                          AAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACG                                                                   >  SRR4302535.3720284/1‑94 (MQ=60)
                           AGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGT                                                                  >  SRR4302535.2504833/1‑94 (MQ=60)
                            GACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTC                                                                 <  SRR4302535.525852/94‑1 (MQ=60)
                             ACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCA                                                                <  SRR4302535.831199/94‑1 (MQ=60)
                             ACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCA                                                                >  SRR4302535.4428403/1‑94 (MQ=60)
                               CTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATT                                                              <  SRR4302535.1184482/94‑1 (MQ=60)
                                  CCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCAC                                                           <  SRR4302535.30435/94‑1 (MQ=60)
                                  CCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCAC                                                           <  SRR4302535.2395492/94‑1 (MQ=60)
                                   CACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACC                                                          <  SRR4302535.2009441/94‑1 (MQ=60)
                                      ACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCT                                                       <  SRR4302535.3581339/94‑1 (MQ=60)
                                       CGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTT                                                      >  SRR4302535.3374647/1‑94 (MQ=60)
                                        GCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTC                                                     >  SRR4302535.2808802/1‑94 (MQ=60)
                                         CAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCA                                                    <  SRR4302535.4195111/94‑1 (MQ=60)
                                          AAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAA                                                   <  SRR4302535.2691165/94‑1 (MQ=60)
                                            GGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACC                                                 <  SRR4302535.4102081/94‑1 (MQ=60)
                                             GCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGATCCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCA                                                <  SRR4302535.4195875/94‑1 (MQ=60)
                                               ATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACC                                              >  SRR4302535.2713809/1‑94 (MQ=60)
                                               ATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACC                                              >  SRR4302535.3461151/1‑94 (MQ=60)
                                               ATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACC                                              <  SRR4302535.1515955/94‑1 (MQ=60)
                                                  GCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCT                                           <  SRR4302535.386991/94‑1 (MQ=60)
                                                  GCCCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCAAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCT                                           <  SRR4302535.1284970/94‑1 (MQ=60)
                                                   CGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTT                                          >  SRR4302535.1523847/1‑94 (MQ=60)
                                                    cccCAATTTGCGCCTGATGCTTGTTTTTCCCCGCCAGTGCCTGAGCCTGACTGGCTGCCCGCGCCCCGTCATTCACCGCTTCAACCACCGCTTT                                         <  SRR4302535.1112466/91‑1 (MQ=60)
                                                    GCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTT                                         >  SRR4302535.1561115/1‑94 (MQ=60)
                                                      TCAATTTGCGCCGGATGCTTGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGT                                       <  SRR4302535.3048791/94‑1 (MQ=60)
                                                        AATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTG                                     >  SRR4302535.3001721/1‑94 (MQ=60)
                                                         ATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGT                                    >  SRR4302535.900122/1‑94 (MQ=60)
                                                         ATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGT                                    <  SRR4302535.2186614/94‑1 (MQ=60)
                                                          TTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTA                                   <  SRR4302535.1600861/94‑1 (MQ=60)
                                                           TTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAG                                  <  SRR4302535.1617309/94‑1 (MQ=60)
                                                              CGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTG                               >  SRR4302535.4068874/1‑94 (MQ=60)
                                                               GCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGG                              <  SRR4302535.1531345/94‑1 (MQ=60)
                                                                CCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGC                             >  SRR4302535.1398135/1‑94 (MQ=60)
                                                                CCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGC                             >  SRR4302535.1372249/1‑94 (MQ=60)
                                                                CCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGC                             >  SRR4302535.4410052/1‑94 (MQ=60)
                                                                CCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGC                             <  SRR4302535.381026/94‑1 (MQ=60)
                                                                    ATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATA                         >  SRR4302535.2492923/1‑94 (MQ=60)
                                                                    ATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATA                         >  SRR4302535.1566348/1‑94 (MQ=60)
                                                                     TGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAG                        <  SRR4302535.3352111/94‑1 (MQ=60)
                                                                     TGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAG                        <  SRR4302535.503498/94‑1 (MQ=60)
                                                                     TGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAG                        <  SRR4302535.99313/94‑1 (MQ=60)
                                                                      GCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGA                       >  SRR4302535.608681/1‑94 (MQ=60)
                                                                       CTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGAC                      <  SRR4302535.3850664/94‑1 (MQ=60)
                                                                         GGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAA                    >  SRR4302535.4237446/1‑94 (MQ=60)
                                                                           TTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGT                  >  SRR4302535.2709268/1‑94 (MQ=60)
                                                                            TTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTT                 <  SRR4302535.360563/94‑1 (MQ=60)
                                                                            TTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTT                 <  SRR4302535.991966/94‑1 (MQ=60)
                                                                            TTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTT                 <  SRR4302535.4307492/94‑1 (MQ=60)
                                                                             TTTCTCCGCCAGTGCCTGAACCTGACTGTCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTG                <  SRR4302535.529382/94‑1 (MQ=60)
                                                                             TTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTG                >  SRR4302535.3190596/1‑94 (MQ=60)
                                                                             TGTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTG                <  SRR4302535.1724322/94‑1 (MQ=60)
                                                                              TTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGC               <  SRR4302535.2888478/94‑1 (MQ=60)
                                                                               TCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCT              <  SRR4302535.2089990/94‑1 (MQ=60)
                                                                               TCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCT              <  SRR4302535.1776393/94‑1 (MQ=60)
                                                                                CTCCGCCAGTGCCTGACCCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTT             <  SRR4302535.455450/94‑1 (MQ=60)
                                                                                CTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTT             <  SRR4302535.4539582/94‑1 (MQ=60)
                                                                                 TCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTT            <  SRR4302535.3825601/94‑1 (MQ=60)
                                                                                 TCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTT            <  SRR4302535.2037590/94‑1 (MQ=60)
                                                                                  CCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTC           <  SRR4302535.1193421/94‑1 (MQ=60)
                                                                                   CGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCG          >  SRR4302535.639441/1‑94 (MQ=60)
                                                                                   CGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGCTGCTTTCG          >  SRR4302535.1239760/1‑94 (MQ=60)
                                                                                    GCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGG         >  SRR4302535.1902058/1‑94 (MQ=60)
                                                                                    GCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGG         <  SRR4302535.3633898/94‑1 (MQ=60)
                                                                                     CCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGC        >  SRR4302535.549469/1‑94 (MQ=60)
                                                                                      CAGTGCCTGAACCTGACTGGCTGCCCGCGCCCCGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGCCAAGTTGCTTTCGGCT       >  SRR4302535.3188797/1‑94 (MQ=60)
                                                                                      CAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCT       <  SRR4302535.3499839/94‑1 (MQ=60)
                                                                                       AGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTT      <  SRR4302535.3907842/94‑1 (MQ=60)
                                                                                       AGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTT      >  SRR4302535.1510882/1‑94 (MQ=60)
                                                                                        GTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTT     <  SRR4302535.1515516/94‑1 (MQ=60)
                                                                                        GTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTT     <  SRR4302535.1136808/94‑1 (MQ=60)
                                                                                         TGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTT    <  SRR4302535.2144219/94‑1 (MQ=60)
                                                                                           CCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGATAAGTTGCTTTCGGCTTTTGC  <  SRR4302535.1636416/94‑1 (MQ=60)
                                                                                           CCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTGC  <  SRR4302535.1727116/94‑1 (MQ=60)
                                                                                           |                                                                                             
ATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTGC  >  NC_000913/2225479‑2225663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑