Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,225,570 T→C T324A (ACA→GCA)  yohG ← putative channel/filament proteins

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,225,5700TC99.6% 742.1 / ‑5.7 231T324A (ACA→GCA) yohGputative channel/filament proteins
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (113/117);  total (113/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

CCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTT  >  NC_000913/2225482‑2225659
                                                                                        |                                                                                         
  cgcgTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAAc                                                                                     >  2:7421613/1‑93 (MQ=255)
  cgcgTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAAc                                                                                     <  2:6985610/93‑1 (MQ=255)
   gcgTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAAcc                                                                                    >  1:4623420/1‑93 (MQ=255)
   gcgTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAAcc                                                                                    >  1:6305445/1‑93 (MQ=255)
     gTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTg                                                                                  >  2:2035333/1‑93 (MQ=255)
      ttAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGa                                                                                 <  2:2975903/93‑1 (MQ=255)
       tAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGAc                                                                                <  2:3331992/93‑1 (MQ=255)
        aaaGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACt                                                                               <  1:6154368/93‑1 (MQ=255)
           gcgcgcCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGc                                                                            <  2:563161/93‑1 (MQ=255)
                 cTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCcg                                                                      >  2:389237/1‑93 (MQ=255)
                 cTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCcg                                                                      >  1:2842049/1‑93 (MQ=255)
                   gcgcAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCcgcg                                                                    >  1:2542558/1‑93 (MQ=255)
                      cAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCa                                                                 >  2:1491480/1‑93 (MQ=255)
                      cAAGACCTACCACACGCAAGGCATCGCGCTAAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCa                                                                 <  2:2683400/93‑1 (MQ=255)
                       caGACCCACCCCACGCAAGGGATCGCGCTCAAATTTCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCAc                                                                <  1:6486203/92‑1 (MQ=255)
                       aaGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCAc                                                                >  1:4020189/1‑93 (MQ=255)
                        aGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACg                                                               >  2:3284743/1‑93 (MQ=255)
                        aGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACg                                                               <  1:3607933/93‑1 (MQ=255)
                          aCCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTc                                                             <  1:4152384/93‑1 (MQ=255)
                          aCCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTc                                                             <  2:2452694/93‑1 (MQ=255)
                            ctcccACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGTCTGCCCGCGCCACGTCAt                                                           <  2:2555174/90‑1 (MQ=255)
                            cTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAt                                                           <  1:2026945/93‑1 (MQ=255)
                            cTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAt                                                           >  1:5010714/1‑93 (MQ=255)
                            cTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAt                                                           >  1:4904691/1‑93 (MQ=255)
                             tACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGGACCTGACTGGCTGCCCGCGCCACGTCAtt                                                          >  1:2362205/1‑93 (MQ=255)
                             tACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAtt                                                          <  2:5967850/93‑1 (MQ=255)
                             tACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAtt                                                          >  1:1772085/1‑93 (MQ=255)
                              aCCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTc                                                         >  1:3534934/1‑93 (MQ=255)
                              aCCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTc                                                         <  1:865894/93‑1 (MQ=255)
                                cacaCGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCAc                                                       <  1:3249960/93‑1 (MQ=255)
                                cacaCGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCAc                                                       <  2:2516693/93‑1 (MQ=255)
                                  cacGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCg                                                     >  2:319326/1‑93 (MQ=255)
                                  cacGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCg                                                     <  1:4053630/93‑1 (MQ=255)
                                     gCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAAACTGACTGGCTGGCCGCGCCACGTCCTTCACCGCtt                                                  >  1:2971012/1‑93 (MQ=255)
                                      cAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTc                                                 <  1:6922317/93‑1 (MQ=255)
                                       aaGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCa                                                >  1:7934107/1‑93 (MQ=255)
                                        aGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCaa                                               <  2:7914459/93‑1 (MQ=255)
                                          gCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAacc                                             <  2:671923/93‑1 (MQ=255)
                                           cATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAacca                                            >  1:2782873/1‑93 (MQ=255)
                                           cATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAacca                                            <  1:7177240/93‑1 (MQ=255)
                                            aTCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAaccac                                           >  1:4479076/1‑93 (MQ=255)
                                             tCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAaccacc                                          >  1:7294753/1‑93 (MQ=255)
                                               gcgcTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGc                                        >  2:6288873/1‑93 (MQ=255)
                                                 gcTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCtt                                      <  1:5297145/93‑1 (MQ=255)
                                                 gcTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCtt                                      >  1:751652/1‑93 (MQ=255)
                                                  cTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCttt                                     <  2:5318124/93‑1 (MQ=255)
                                                  cTCAATTTGCGCCTAATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCttt                                     >  2:4153449/1‑93 (MQ=255)
                                                  cTCAACTTGCGCGTGATGCTGGTTTTGCTCCGCCCGCGCCTGAACCTGACTGGCTGTCAGCGCCACGTCATTCACCGCTTCAACCCCCGCttt                                     <  2:5318121/93‑1 (MQ=255)
                                                   tCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTttg                                    >  2:1433879/1‑93 (MQ=255)
                                                     aaTTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTttgtt                                  >  1:7035414/1‑93 (MQ=255)
                                                      aTTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTttgttg                                 >  1:135357/1‑93 (MQ=255)
                                                      aTTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTttgttg                                 <  1:1486934/93‑1 (MQ=255)
                                                       tttGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTtgttgt                                <  2:7748898/93‑1 (MQ=255)
                                                        ttGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTa                               <  1:341443/93‑1 (MQ=255)
                                                         tGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAg                              <  2:2310493/93‑1 (MQ=255)
                                                         tGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAg                              <  2:6669503/93‑1 (MQ=255)
                                                         tGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAg                              >  1:987165/1‑93 (MQ=255)
                                                         tGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAg                              <  1:5380187/93‑1 (MQ=255)
                                                          gcgcCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGc                             <  1:1798381/93‑1 (MQ=255)
                                                          gcgcCTGATGCGGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGc                             <  2:2417090/93‑1 (MQ=255)
                                                           cgcCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCt                            <  1:5264014/93‑1 (MQ=255)
                                                            gcCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTg                           <  2:1268737/93‑1 (MQ=255)
                                                            gcCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTg                           <  2:48579/93‑1 (MQ=255)
                                                            gcCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTg                           <  1:6188613/93‑1 (MQ=255)
                                                             ccTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTgg                          >  2:4747769/1‑93 (MQ=255)
                                                             ccTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTgg                          <  2:4481385/93‑1 (MQ=255)
                                                             ccTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTgg                          <  1:2538651/93‑1 (MQ=255)
                                                             ccTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTgg                          >  2:2053956/1‑93 (MQ=255)
                                                              cTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGc                         <  2:3968744/93‑1 (MQ=255)
                                                              cTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGc                         >  2:1408787/1‑93 (MQ=255)
                                                               tGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCg                        >  1:3295779/1‑93 (MQ=255)
                                                               tGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCg                        >  2:5019547/1‑93 (MQ=255)
                                                                gATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGa                       >  1:4790983/1‑93 (MQ=255)
                                                                gATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGa                       <  1:2003474/93‑1 (MQ=255)
                                                                gATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGa                       <  1:7794134/93‑1 (MQ=255)
                                                                gATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGGGATTCACCGCTTCAACCACCGCTTTGTTGTTGCTggtgg                       >  2:6196029/1‑90 (MQ=255)
                                                                 ttGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGAt                      <  1:1881751/92‑1 (MQ=255)
                                                                 aTGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGAt                      <  2:7615088/93‑1 (MQ=255)
                                                                  tGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATa                     >  1:977149/1‑93 (MQ=255)
                                                                   gCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAg                    >  1:4063832/1‑93 (MQ=255)
                                                                   gCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAg                    <  2:2865427/93‑1 (MQ=255)
                                                                     tGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGaa                  >  1:1533855/1‑92 (MQ=255)
                                                                     tGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGAc                  <  2:4401739/93‑1 (MQ=255)
                                                                      ggTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACa                 <  2:5703924/93‑1 (MQ=255)
                                                                          tttCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGtt             <  1:642383/93‑1 (MQ=255)
                                                                            tctcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGc           <  1:6653049/93‑1 (MQ=255)
                                                                            tctcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGc           <  2:797238/93‑1 (MQ=255)
                                                                            tctcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGc           >  2:2004757/1‑93 (MQ=255)
                                                                             ctcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAGCCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCt          <  1:6599389/93‑1 (MQ=255)
                                                                              tcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCtt         >  2:5565248/1‑93 (MQ=255)
                                                                              tcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCtt         >  1:4605158/1‑93 (MQ=255)
                                                                              tcCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCtt         <  2:3724828/93‑1 (MQ=255)
                                                                               ccGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCttt        <  1:5677934/93‑1 (MQ=255)
                                                                                 gCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCg      >  2:6630220/1‑93 (MQ=255)
                                                                                  ccAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCgg     >  2:1488268/1‑93 (MQ=255)
                                                                                  ccAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCgg     >  2:3511536/1‑93 (MQ=255)
                                                                                  ccAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCgg     <  1:3939168/93‑1 (MQ=255)
                                                                                    aGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCt   <  1:6870215/93‑1 (MQ=255)
                                                                                    aGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCt   >  2:5140463/1‑93 (MQ=255)
                                                                                     gtgcCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCtt  >  2:6479405/1‑93 (MQ=255)
                                                                                        |                                                                                         
CCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTT  >  NC_000913/2225482‑2225659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTGC  >  NC_000913/2225477‑2225663
                                                                                             |                                                                                             
TGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGC                                                                                               >  SRR4302543.895424/1‑94 (MQ=60)
 GATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCC                                                                                              <  SRR4302543.3464925/94‑1 (MQ=60)
 GATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCC                                                                                              >  SRR4302543.4136008/1‑94 (MQ=60)
  ATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCT                                                                                             <  SRR4302543.935470/94‑1 (MQ=60)
      CCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAAC                                                                                         >  SRR4302543.5403336/1‑94 (MQ=60)
      CCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAAC                                                                                         <  SRR4302543.1544334/94‑1 (MQ=60)
       CGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACC                                                                                        >  SRR4302543.4665423/1‑94 (MQ=60)
       CGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACC                                                                                        >  SRR4302543.6364757/1‑94 (MQ=60)
       CGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACC                                                                                        >  SRR4302543.4665424/1‑94 (MQ=60)
        GCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCT                                                                                       <  SRR4302543.6713202/94‑1 (MQ=60)
          GTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGA                                                                                     >  SRR4302543.7486016/1‑94 (MQ=60)
           TTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGAC                                                                                    <  SRR4302543.4717371/94‑1 (MQ=60)
           TTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGAC                                                                                    <  SRR4302543.5020925/94‑1 (MQ=60)
           TTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGAC                                                                                    >  SRR4302543.994149/1‑94 (MQ=60)
            TAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACT                                                                                   <  SRR4302543.3388308/94‑1 (MQ=60)
             AAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTG                                                                                  <  SRR4302543.6212626/94‑1 (MQ=60)
             AAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTG                                                                                  <  SRR4302543.4154609/94‑1 (MQ=60)
               AGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGC                                                                                >  SRR4302543.7309333/1‑94 (MQ=60)
                GCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCT                                                                               <  SRR4302543.1842484/94‑1 (MQ=60)
                   CGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCC                                                                            >  SRR4302543.2979938/1‑94 (MQ=60)
                    GCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCC                                                                           <  SRR4302543.6878776/94‑1 (MQ=60)
                     CCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCG                                                                          >  SRR4302543.2868020/1‑94 (MQ=60)
                       TGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCG                                                                        >  SRR4302543.2565739/1‑94 (MQ=60)
                       TGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCG                                                                        >  SRR4302543.2565740/1‑94 (MQ=60)
                       TGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCACG                                                                        >  SRR4302543.833796/1‑94 (MQ=60)
                         CGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCC                                                                      <  SRR4302543.221640/94‑1 (MQ=60)
                           CAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCAC                                                                    >  SRR4302543.4056098/1‑94 (MQ=60)
                           CAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCAC                                                                    >  SRR4302543.4058356/1‑94 (MQ=60)
                            CAGACCCACCCCACGCAAGGGATCGCGCTCAAATTTCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACG                                                                   <  SRR4302543.6547315/94‑1 (MQ=60)
                             AGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGT                                                                  >  SRR4302543.5192061/1‑94 (MQ=60)
                             AGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGT                                                                  <  SRR4302543.3639769/94‑1 (MQ=60)
                               ACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCA                                                                <  SRR4302543.4189925/94‑1 (MQ=60)
                                CCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAT                                                               >  SRR4302543.3491707/1‑94 (MQ=60)
                                CCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAT                                                               >  SRR4302543.4949393/1‑94 (MQ=60)
                                CCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAT                                                               >  SRR4302543.5056317/1‑94 (MQ=60)
                                CCCACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCAT                                                               <  SRR4302543.6829728/94‑1 (MQ=60)
                                 CTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGGACCTGACTGGCTGCCCGCGCCACGTCATT                                                              >  SRR4302543.2383627/1‑94 (MQ=60)
                                 CTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATT                                                              <  SRR4302543.2045160/94‑1 (MQ=60)
                                 CTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATT                                                              >  SRR4302543.1787925/1‑94 (MQ=60)
                                 CTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATT                                                              >  SRR4302543.1614904/1‑94 (MQ=60)
                                  TACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTC                                                             >  SRR4302543.3566388/1‑94 (MQ=60)
                                  TACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTC                                                             <  SRR4302543.1442692/94‑1 (MQ=60)
                                   ACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCA                                                            <  SRR4302543.873634/94‑1 (MQ=60)
                                     CACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACC                                                          >  SRR4302543.6582011/1‑94 (MQ=60)
                                     CACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACC                                                          >  SRR4302543.7168393/1‑94 (MQ=60)
                                     CACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACC                                                          <  SRR4302543.7984294/94‑1 (MQ=60)
                                     CACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACC                                                          <  SRR4302543.3279343/94‑1 (MQ=60)
                                       CACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGC                                                        <  SRR4302543.4089908/94‑1 (MQ=60)
                                         CGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTT                                                      >  SRR4302543.2997993/1‑94 (MQ=60)
                                         CGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAAACTGACTGGCTGGCCGCGCCACGTCCTTCACCGCTT                                                      >  SRR4302543.2997992/1‑94 (MQ=60)
                                           CAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCA                                                    <  SRR4302543.6987408/94‑1 (MQ=60)
                                           CAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCA                                                    >  SRR4302543.8008580/1‑94 (MQ=60)
                                           CAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCA                                                    >  SRR4302543.4526401/1‑94 (MQ=60)
                                           CAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCA                                                    >  SRR4302543.4396685/1‑94 (MQ=60)
                                            AAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAA                                                   >  SRR4302543.1006061/1‑94 (MQ=60)
                                              GGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACC                                                 <  SRR4302543.1805650/94‑1 (MQ=60)
                                               GCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCA                                                >  SRR4302543.2808246/1‑94 (MQ=60)
                                                CATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCAC                                               <  SRR4302543.7246911/94‑1 (MQ=60)
                                                CATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCAC                                               >  SRR4302543.4519746/1‑94 (MQ=60)
                                                CATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCAC                                               >  SRR4302543.2493344/1‑94 (MQ=60)
                                                 ATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACC                                              >  SRR4302543.3482519/1‑94 (MQ=60)
                                                 ATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACC                                              >  SRR4302543.7727967/1‑94 (MQ=60)
                                                 ATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACC                                              >  SRR4302543.3949350/1‑94 (MQ=60)
                                                 ATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACC                                              >  SRR4302543.7365144/1‑94 (MQ=60)
                                                  TCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCG                                             <  SRR4302543.2614630/94‑1 (MQ=60)
                                                   CGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGC                                            >  SRR4302543.6532721/1‑94 (MQ=60)
                                                     CGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTT                                          >  SRR4302543.758318/1‑94 (MQ=60)
                                                      GCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTT                                         <  SRR4302543.5344965/94‑1 (MQ=60)
                                                       CTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTTCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTG                                        <  SRR4302543.2629943/94‑1 (MQ=60)
                                                       CTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTG                                        >  SRR4302543.5251920/1‑94 (MQ=60)
                                                       CTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTG                                        >  SRR4302543.6412014/1‑94 (MQ=60)
                                                         CAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTT                                      >  SRR4302543.7445084/1‑94 (MQ=60)
                                                         CAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTT                                      >  SRR4302543.2039869/1‑94 (MQ=60)
                                                         CAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTT                                      >  SRR4302543.7103160/1‑94 (MQ=60)
                                                          AATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTG                                     >  SRR4302543.136554/1‑94 (MQ=60)
                                                          AATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTG                                     >  SRR4302543.850768/1‑94 (MQ=60)
                                                           ATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGT                                    <  SRR4302543.1500312/94‑1 (MQ=60)
                                                            TTTGCGCTTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTA                                   <  SRR4302543.5661392/94‑1 (MQ=60)
                                                             TTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAG                                  >  SRR4302543.995874/1‑94 (MQ=60)
                                                             TTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAG                                  <  SRR4302543.344586/94‑1 (MQ=60)
                                                              TGCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGC                                 <  SRR4302543.5428983/94‑1 (MQ=60)
                                                               GCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCT                                <  SRR4302543.3737463/94‑1 (MQ=60)
                                                               GCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCT                                <  SRR4302543.903297/94‑1 (MQ=60)
                                                               GCGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCT                                <  SRR4302543.1814485/94‑1 (MQ=60)
                                                                CGCCTTATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCTCTTTGTTGTAGCTG                               <  SRR4302543.5311508/94‑1 (MQ=60)
                                                                CGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTG                               <  SRR4302543.2010252/94‑1 (MQ=60)
                                                                CGCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTG                               <  SRR4302543.5311507/94‑1 (MQ=60)
                                                                 GCCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGG                              <  SRR4302543.6247082/94‑1 (MQ=60)
                                                                  CCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGC                             >  SRR4302543.3212403/1‑94 (MQ=60)
                                                                  CCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGC                             <  SRR4302543.2561788/94‑1 (MQ=60)
                                                                  CCTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGC                             <  SRR4302543.4635646/94‑1 (MQ=60)
                                                                   CTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCG                            >  SRR4302543.3325513/1‑94 (MQ=60)
                                                                   CTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCG                            >  SRR4302543.4003210/1‑94 (MQ=60)
                                                                   CTGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCACGCGCCACGTCCTTCACCGCTTNAANNACCNCTTTGTTNTANCNGNCG                            >  SRR4302543.1392794/1‑94 (MQ=60)
                                                                    TGATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGA                           >  SRR4302543.4834774/1‑94 (MQ=60)
                                                                     GATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGAT                          <  SRR4302543.2021471/94‑1 (MQ=60)
                                                                     GATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGAT                          <  SRR4302543.7867587/94‑1 (MQ=60)
                                                                      TTGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATA                         <  SRR4302543.1898659/94‑1 (MQ=60)
                                                                      ATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATA                         >  SRR4302543.2417692/1‑94 (MQ=60)
                                                                      ATGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATA                         >  SRR4302543.985767/1‑94 (MQ=60)
                                                                       TGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAG                        >  SRR4302543.1964907/1‑94 (MQ=60)
                                                                       TGCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAG                        >  SRR4302543.4100219/1‑94 (MQ=60)
                                                                        GCTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGA                       <  SRR4302543.3636021/94‑1 (MQ=60)
                                                                         CTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGAC                      >  SRR4302543.6942963/1‑94 (MQ=60)
                                                                         CTGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGAA                      >  SRR4302543.1547598/1‑94 (MQ=60)
                                                                          TGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACA                     <  SRR4302543.4095977/94‑1 (MQ=60)
                                                                          TGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACA                     >  SRR4302543.6348555/1‑94 (MQ=60)
                                                                          TGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACA                     <  SRR4302543.2861854/94‑1 (MQ=60)
                                                                          TGGTTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACA                     >  SRR4302543.5529507/1‑94 (MQ=60)
                                                                             TTTTTCTCCGCTAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCACTCACCGCTTCAACCACCGCTTTGTTGTAGCTTGCGATAGACAAGT                  >  SRR4302543.7100407/1‑94 (MQ=60)
                                                                             TTTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGT                  <  SRR4302543.1559465/94‑1 (MQ=60)
                                                                              TTTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTT                 >  SRR4302543.4929177/1‑94 (MQ=60)
                                                                               TTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTG                <  SRR4302543.648244/94‑1 (MQ=60)
                                                                               TTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTG                <  SRR4302543.7165704/94‑1 (MQ=60)
                                                                               TTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTG                <  SRR4302543.6223745/94‑1 (MQ=60)
                                                                               TTTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTG                <  SRR4302543.648245/94‑1 (MQ=60)
                                                                                TTCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGC               <  SRR4302543.3301894/94‑1 (MQ=60)
                                                                                 TCTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCT              <  SRR4302543.6715856/94‑1 (MQ=60)
                                                                                  CTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAGCCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTT             <  SRR4302543.6661576/94‑1 (MQ=60)
                                                                                  CTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTT             <  SRR4302543.4854610/94‑1 (MQ=60)
                                                                                  CTCCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTT             >  SRR4302543.4646990/1‑94 (MQ=60)
                                                                                    CCGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTC           <  SRR4302543.5729762/94‑1 (MQ=60)
                                                                                     CGCCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCG          <  SRR4302543.4100142/94‑1 (MQ=60)
                                                                                       CCAGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGC        <  SRR4302543.3973990/94‑1 (MQ=60)
                                                                                         AGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTT      <  SRR4302543.6934925/94‑1 (MQ=60)
                                                                                         AGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTT      >  SRR4302543.5942593/1‑94 (MQ=60)
                                                                                         AGTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTT      >  SRR4302543.6271093/1‑94 (MQ=60)
                                                                                          GTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTT     >  SRR4302543.7279784/1‑94 (MQ=60)
                                                                                          GTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTT     >  SRR4302543.1390266/1‑94 (MQ=60)
                                                                                          GTGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTCCTTTCGGCTTT     >  SRR4302543.2120192/1‑94 (MQ=60)
                                                                                           TGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTT    >  SRR4302543.7529153/1‑94 (MQ=60)
                                                                                           TGCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTT    <  SRR4302543.3362704/94‑1 (MQ=60)
                                                                                            GCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTG   >  SRR4302543.6363708/1‑94 (MQ=60)
                                                                                            GCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTG   <  SRR4302543.550839/94‑1 (MQ=60)
                                                                                            GCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTG   <  SRR4302543.4497624/94‑1 (MQ=60)
                                                                                            GCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTG   >  SRR4302543.6747490/1‑94 (MQ=60)
                                                                                            GCCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTG   <  SRR4302543.4706407/94‑1 (MQ=60)
                                                                                             CCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTGC  >  SRR4302543.5284497/1‑94 (MQ=60)
                                                                                             CCTGAACCTGACTGGCTGCCCGCGCCACGTAATTCGCCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTGC  <  SRR4302543.3511239/94‑1 (MQ=60)
                                                                                             |                                                                                             
TGATGCCCGCGTTAAAGCGCGCCTGCGCAAGACCTACCACACGCAAGGCATCGCGCTCAATTTGCGCCTGATGCTGGTTTTTCTCCGCCAGTGTCTGAACCTGACTGGCTGCCCGCGCCACGTCATTCACCGCTTCAACCACCGCTTTGTTGTAGCTGGCGATAGACAAGTTGCTTTCGGCTTTTGC  >  NC_000913/2225477‑2225663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑