Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,261,454 G→A A21V (GCG→GTG)  fruB ← PTS system, fructose‑specific IIA/fpr component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,261,4540GA100.0% 505.9 / NA 152A21V (GCG→GTG) fruBPTS system, fructose‑specific IIA/fpr component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (78/74);  total (78/74)

Only 100 of 155 total aligned reads displayed.

GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC  >  NC_000913/2261364‑2261542
                                                                                          |                                                                                        
gAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAcc                                                                                        >  2:1275163/1‑93 (MQ=255)
gAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAcc                                                                                        >  1:4490203/1‑93 (MQ=255)
 aGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACct                                                                                       >  2:3683126/1‑93 (MQ=255)
  ggTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctc                                                                                      >  2:4672899/1‑93 (MQ=255)
  ggTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctc                                                                                      <  1:1881181/93‑1 (MQ=255)
     ttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttt                                                                                   >  1:310161/1‑92 (MQ=255)
      tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                  <  2:1959297/93‑1 (MQ=255)
      tGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttct                                                                                  <  2:3196846/93‑1 (MQ=255)
       gCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTcttctt                                                                                 >  1:2433851/1‑93 (MQ=255)
        cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCttt                                                                                >  1:3642265/1‑93 (MQ=255)
         tGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTg                                                                               >  2:3786373/1‑93 (MQ=255)
          gTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGt                                                                              >  1:2198312/1‑93 (MQ=255)
           ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtc                                                                             <  1:1533613/93‑1 (MQ=255)
           ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtc                                                                             >  1:36130/1‑93 (MQ=255)
             cgcgcgCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtctc                                                                           <  1:1585727/93‑1 (MQ=255)
              gcgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTcc                                                                          <  1:378636/93‑1 (MQ=255)
                gcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCgg                                                                        >  1:4080448/1‑93 (MQ=255)
                gcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCgg                                                                        <  1:1660444/93‑1 (MQ=255)
                  gcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGcc                                                                      <  1:671888/93‑1 (MQ=255)
                  gcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGcc                                                                      >  1:857153/1‑93 (MQ=255)
                   ccAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCt                                                                     >  1:2523861/1‑93 (MQ=255)
                    cAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtt                                                                    >  1:2467015/1‑93 (MQ=255)
                     aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                   >  1:4354753/1‑93 (MQ=255)
                     aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                   >  2:764247/1‑93 (MQ=255)
                     aGCATGCCATTGACGTACCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCttt                                                                   >  2:1823210/1‑93 (MQ=255)
                      gCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtttt                                                                  >  2:2338034/1‑93 (MQ=255)
                      gCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtttt                                                                  >  1:535141/1‑93 (MQ=255)
                       cATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTc                                                                 <  2:3472362/93‑1 (MQ=255)
                       cATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTc                                                                 <  2:480630/93‑1 (MQ=255)
                        aTGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCg                                                                >  2:2390980/1‑93 (MQ=255)
                         tGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGc                                                               >  2:4659079/1‑93 (MQ=255)
                         tGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGACGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGc                                                               >  1:3041975/1‑93 (MQ=255)
                           ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGccc                                                             >  1:3368912/1‑93 (MQ=255)
                           ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGccc                                                             >  1:2647763/1‑93 (MQ=255)
                           ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGccc                                                             <  2:4591212/93‑1 (MQ=255)
                           ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTAGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGcac                                                             >  2:2920492/1‑91 (MQ=255)
                            cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCg                                                            >  2:4423600/1‑93 (MQ=255)
                             aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGcccag                                                           >  2:35364/1‑91 (MQ=255)
                             aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgg                                                           >  1:487935/1‑93 (MQ=255)
                             aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGACTTTTCGCCCgg                                                           >  2:4101226/1‑93 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                          >  1:4493424/1‑93 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                          >  1:3397795/1‑93 (MQ=255)
                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                          <  1:1022566/93‑1 (MQ=255)
                               tGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggat                                                         <  2:2583851/93‑1 (MQ=255)
                               tGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggat                                                         <  2:2244148/93‑1 (MQ=255)
                                gACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatg                                                        >  1:1722053/1‑93 (MQ=255)
                                 aCGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCTggatgg                                                       <  2:1488792/93‑1 (MQ=255)
                                  cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                      <  2:1579190/93‑1 (MQ=255)
                                  cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                      <  1:3313749/93‑1 (MQ=255)
                                  cGTAACCTTCTGCTACATTACCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                      <  2:1691971/93‑1 (MQ=255)
                                   gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatggat                                                     <  1:1793511/93‑1 (MQ=255)
                                   gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatggat                                                     <  1:2723366/93‑1 (MQ=255)
                                     aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGt                                                   <  1:4370785/93‑1 (MQ=255)
                                     aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGt                                                   <  1:4255055/93‑1 (MQ=255)
                                        cTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCt                                                >  2:1251791/1‑93 (MQ=255)
                                         ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                               >  1:2251738/1‑93 (MQ=255)
                                         ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg                                               >  1:2363657/1‑93 (MQ=255)
                                          tCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGt                                              <  1:4598150/93‑1 (MQ=255)
                                          tCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGt                                              >  2:1346182/1‑93 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                         >  1:2906752/1‑93 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                         <  1:465680/93‑1 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                         >  2:2650458/1‑93 (MQ=255)
                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                         <  1:3260222/93‑1 (MQ=255)
                                                aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAt                                        <  1:1678918/93‑1 (MQ=255)
                                                 cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATa                                       <  1:1579684/93‑1 (MQ=255)
                                                 cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATa                                       >  2:816748/1‑93 (MQ=255)
                                                  aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                      >  1:2326138/1‑93 (MQ=255)
                                                   ttACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTACTGTACGGATAAc                                     >  1:4080760/1‑93 (MQ=255)
                                                      ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg                                  <  2:3254596/93‑1 (MQ=255)
                                                          ccTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa                              <  1:1470211/93‑1 (MQ=255)
                                                           cTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAt                             >  2:1201775/1‑93 (MQ=255)
                                                             gCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAg                           <  1:2489547/93‑1 (MQ=255)
                                                             gCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAg                           >  1:3248834/1‑93 (MQ=255)
                                                             gCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAg                           >  1:3422153/1‑93 (MQ=255)
                                                              cACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGt                          <  2:4353137/93‑1 (MQ=255)
                                                              cACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGt                          <  1:2938770/93‑1 (MQ=255)
                                                               aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                         <  1:2428792/93‑1 (MQ=255)
                                                               aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                         >  2:2280336/1‑93 (MQ=255)
                                                               aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                         >  1:2764096/1‑93 (MQ=255)
                                                                ccAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                        <  2:2938990/93‑1 (MQ=255)
                                                                ccAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                        <  2:3164756/93‑1 (MQ=255)
                                                                   gcgcCGCAGCGACCTGGCGAATCACCTCTTCTTTGACTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                     <  1:2619871/93‑1 (MQ=255)
                                                                     gccgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcctc                   <  1:4236594/93‑1 (MQ=255)
                                                                     gccgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcctc                   <  1:2933221/93‑1 (MQ=255)
                                                                       cgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctc                 <  1:1217807/93‑1 (MQ=255)
                                                                        gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                <  2:4188099/93‑1 (MQ=255)
                                                                        gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                >  2:800750/1‑93 (MQ=255)
                                                                         cAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCtt               <  1:1301738/93‑1 (MQ=255)
                                                                          aGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg              >  2:1699410/1‑93 (MQ=255)
                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc             >  1:532581/1‑93 (MQ=255)
                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc             >  2:225775/1‑93 (MQ=255)
                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCACCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc             >  2:2425358/1‑93 (MQ=255)
                                                                              aCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa          >  2:957822/1‑93 (MQ=255)
                                                                              aCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa          <  2:2191717/93‑1 (MQ=255)
                                                                                cTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt        >  2:392600/1‑93 (MQ=255)
                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt       <  1:1069944/93‑1 (MQ=255)
                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa   <  2:171003/93‑1 (MQ=255)
                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc  >  2:715959/1‑93 (MQ=255)
                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc  <  2:753203/93‑1 (MQ=255)
                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc  <  2:3722718/93‑1 (MQ=255)
                                                                                          |                                                                                        
GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC  >  NC_000913/2261364‑2261542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261362‑2261547
                                                                                            |                                                                                             
TTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAC                                                                                              >  SRR4302535.2206809/1‑94 (MQ=60)
 TGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACC                                                                                             >  SRR4302535.4521026/1‑94 (MQ=60)
    GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          <  SRR4302535.1954058/94‑1 (MQ=60)
    GGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCT                                                                                          <  SRR4302535.1894741/94‑1 (MQ=60)
      TTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTT                                                                                        >  SRR4302535.312312/1‑94 (MQ=60)
        TGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTT                                                                                      >  SRR4302535.2451384/1‑94 (MQ=60)
         GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     >  SRR4302535.3667491/1‑94 (MQ=60)
           TGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGT                                                                                   >  SRR4302535.2213975/1‑94 (MQ=60)
            GTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTC                                                                                  >  SRR4302535.36408/1‑94 (MQ=60)
             TTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCT                                                                                 <  SRR4302535.1544662/94‑1 (MQ=60)
               CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCC                                                                               <  SRR4302535.1597168/94‑1 (MQ=60)
                GCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCG                                                                              <  SRR4302535.381259/94‑1 (MQ=60)
                GCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCG‑ATCACCTCTTCTTTTTCTTCGG                                                                             >  SRR4302535.4592943/1‑94 (MQ=60)
                 CGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGG                                                                             >  SRR4302535.4108359/1‑94 (MQ=60)
                  GCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGC                                                                            <  SRR4302535.1672443/94‑1 (MQ=60)
                  GCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGC                                                                            >  SRR4302535.1558299/1‑94 (MQ=60)
                   CGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCC                                                                           >  SRR4302535.863195/1‑94 (MQ=60)
                    GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          <  SRR4302535.676577/94‑1 (MQ=60)
                    GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          >  SRR4302535.2542048/1‑94 (MQ=60)
                     CCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTT                                                                         >  SRR4302535.2484798/1‑94 (MQ=60)
                      CAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTT                                                                        >  SRR4302535.4384563/1‑94 (MQ=60)
                       AGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTT                                                                       >  SRR4302535.538881/1‑94 (MQ=60)
                        GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      <  SRR4302535.4307/94‑1 (MQ=60)
                        GCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTC                                                                      <  SRR4302535.3616215/94‑1 (MQ=60)
                         CATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCG                                                                     >  SRR4302535.2956053/1‑94 (MQ=60)
                          ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302535.2592475/1‑94 (MQ=60)
                          ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302535.3849998/1‑94 (MQ=60)
                          ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGACGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302535.3063290/1‑94 (MQ=60)
                            GCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCC                                                                  >  SRR4302535.2666728/1‑94 (MQ=60)
                            GCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCC                                                                  >  SRR4302535.3392353/1‑94 (MQ=60)
                              CATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGG                                                                >  SRR4302535.491354/1‑94 (MQ=60)
                              CATTGACGTAGCCTTCTGCGACATTACCGGCCTGCAGCAGCCCCGCCGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGG                                                                <  SRR4302535.137002/94‑1 (MQ=60)
                               ATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGA                                                               >  SRR4302535.3421438/1‑94 (MQ=60)
                               ATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGA                                                               >  SRR4302535.4524267/1‑94 (MQ=60)
                                TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302535.4468521/94‑1 (MQ=60)
                                TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302535.1029830/94‑1 (MQ=60)
                                 TGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATG                                                             >  SRR4302535.1734509/1‑94 (MQ=60)
                                    CGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGAT                                                          <  SRR4302535.3336807/94‑1 (MQ=60)
                                     GTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATG                                                         <  SRR4302535.1806478/94‑1 (MQ=60)
                                     GTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATG                                                         <  SRR4302535.2742820/94‑1 (MQ=60)
                                       AGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTC                                                       <  SRR4302535.4400694/94‑1 (MQ=60)
                                       AGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTC                                                       <  SRR4302535.4284206/94‑1 (MQ=60)
                                         CCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     <  SRR4302535.963372/94‑1 (MQ=60)
                                         CCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     <  SRR4302535.1727067/94‑1 (MQ=60)
                                          CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302535.2267773/1‑94 (MQ=60)
                                          CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    <  SRR4302535.2291301/94‑1 (MQ=60)
                                          CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302535.2494554/1‑94 (MQ=60)
                                          CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302535.2380631/1‑94 (MQ=60)
                                           TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   >  SRR4302535.645343/1‑94 (MQ=60)
                                           TTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGT                                                   >  SRR4302535.2699457/1‑94 (MQ=60)
                                            TCTGCTACATTACCGGCTTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTA                                                  <  SRR4302535.3747646/94‑1 (MQ=60)
                                            TCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTA                                                  <  SRR4302535.4629431/94‑1 (MQ=60)
                                              TGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACG                                                >  SRR4302535.2472971/1‑94 (MQ=60)
                                                CTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGA                                              >  SRR4302535.2927296/1‑94 (MQ=60)
                                                CTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGA                                              >  SRR4302535.2814367/1‑94 (MQ=60)
                                                 TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             <  SRR4302535.468937/94‑1 (MQ=60)
                                                 TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             <  SRR4302535.3282877/94‑1 (MQ=60)
                                                 TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             >  SRR4302535.1615412/1‑94 (MQ=60)
                                                  ACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATA                                            <  SRR4302535.1691074/94‑1 (MQ=60)
                                                   CATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAA                                           >  SRR4302535.2342781/1‑94 (MQ=60)
                                                   CATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAA                                           <  SRR4302535.1591089/94‑1 (MQ=60)
                                                    ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTACTGTACGGATAAC                                          >  SRR4302535.4108673/1‑94 (MQ=60)
                                                      TACCGGCCTGCACCAGCGCCGCAGCGTCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTG                                        <  SRR4302535.1167303/94‑1 (MQ=60)
                                                          GGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAAC                                    >  SRR4302535.3306986/1‑94 (MQ=60)
                                                            CCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT                                  <  SRR4302535.1480741/94‑1 (MQ=60)
                                                              TGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                >  SRR4302535.3271403/1‑94 (MQ=60)
                                                              TGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                >  SRR4302535.3445957/1‑94 (MQ=60)
                                                               GCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGT                               <  SRR4302535.2507500/94‑1 (MQ=60)
                                                                CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              <  SRR4302535.2959441/94‑1 (MQ=60)
                                                                CACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTT                              >  SRR4302535.2783786/1‑94 (MQ=60)
                                                                 ACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTC                             <  SRR4302535.2446293/94‑1 (MQ=60)
                                                                    AGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCC                          >  SRR4302535.3414047/1‑94 (MQ=60)
                                                                     GCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGACTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCT                         <  SRR4302535.2638651/94‑1 (MQ=60)
                                                                       GCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCT                       <  SRR4302535.2953867/94‑1 (MQ=60)
                                                                       GCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCT                       <  SRR4302535.4265588/94‑1 (MQ=60)
                                                                         CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     >  SRR4302535.3154556/1‑94 (MQ=60)
                                                                         CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     <  SRR4302535.1226543/94‑1 (MQ=60)
                                                                         CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     <  SRR4302535.2388327/94‑1 (MQ=60)
                                                                           CAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTG                   <  SRR4302535.1311037/94‑1 (MQ=60)
                                                                           CAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTG                   <  SRR4302535.527997/94‑1 (MQ=60)
                                                                            AGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGC                  >  SRR4302535.536309/1‑94 (MQ=60)
                                                                                 CCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAT             >  SRR4302535.3103381/1‑94 (MQ=60)
                                                                                  CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            <  SRR4302535.1805187/94‑1 (MQ=60)
                                                                                   TGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTG           <  SRR4302535.1077549/94‑1 (MQ=60)
                                                                                   TGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTG           <  SRR4302535.472392/94‑1 (MQ=60)
                                                                                        AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      <  SRR4302535.4190747/94‑1 (MQ=60)
                                                                                          TCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGAT    <  SRR4302535.816832/94‑1 (MQ=60)
                                                                                           CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGACTTGAAACGATT   >  SRR4302535.2133198/1‑94 (MQ=60)
                                                                                            CCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  <  SRR4302535.1077691/94‑1 (MQ=60)
                                                                                            ACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  SRR4302535.2796547/1‑94 (MQ=60)
                                                                                            ACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  <  SRR4302535.3231795/94‑1 (MQ=60)
                                                                                            |                                                                                             
TTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261362‑2261547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑