Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,261,454	G→A	A21V (GCG→GTG)	fruB ←	PTS system, fructose‑specific IIA/fpr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,261,454	0	G	A	98.7%	770.2 / NA	235	A21V (GCG→GTG)	fruB	PTS system, fructose‑specific IIA/fpr component
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (124/107); minor base C (0/3); total (124/111)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC  >  NC_000913/2261364‑2261542

gAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAcc                                                                                        >  1:7524947/1‑93 (MQ=255)

   gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctct                                                                                     <  2:5614441/93‑1 (MQ=255)

   gTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACctct                                                                                     >  2:2983298/1‑93 (MQ=255)

    tttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtctt                                                                                    >  1:5611121/1‑93 (MQ=255)

     ttGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCtcttc                                                                                   >  2:3213885/1‑93 (MQ=255)

        cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTTCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCttt                                                                                <  1:6944375/93‑1 (MQ=255)

        cTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCttt                                                                                >  1:2827526/1‑93 (MQ=255)

           ttCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGtc                                                                             <  2:6323718/93‑1 (MQ=255)

              gcgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTcc                                                                          <  1:7395434/93‑1 (MQ=255)

               cgcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCg                                                                         >  1:2049067/1‑93 (MQ=255)

                   ccAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCt                                                                     >  1:7352797/1‑93 (MQ=255)

                   ccAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCt                                                                     >  1:6341743/1‑93 (MQ=255)

                   ccAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCt                                                                     >  1:6249254/1‑93 (MQ=255)

                   ccAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCt                                                                     >  2:6728974/1‑93 (MQ=255)

                      gCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtttt                                                                  <  2:1707624/93‑1 (MQ=255)

                      gCATGCCATTGACGTAGCCTTCTGCTACATTACCCGCCTGCACCAGCGCCGCAGCGCCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCtttt                                                                  >  1:5497663/1‑93 (MQ=255)

                        aTGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCg                                                                >  2:3511789/1‑93 (MQ=255)

                         tGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGc                                                               >  1:3904258/1‑93 (MQ=255)

                         tGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGc                                                               >  2:4682590/1‑93 (MQ=255)

                         tGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGc                                                               >  1:5412946/1‑93 (MQ=255)

                           ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGccc                                                             <  1:4174102/93‑1 (MQ=255)

                           ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGccc                                                             >  1:6817584/1‑93 (MQ=255)

                            cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCg                                                            >  2:3966345/1‑93 (MQ=255)

                             aTTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgg                                                           >  2:2160146/1‑93 (MQ=255)

                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                          <  2:4208197/93‑1 (MQ=255)

                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                          <  2:4848830/93‑1 (MQ=255)

                              ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga                                                          >  2:82885/1‑93 (MQ=255)

                                  cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                      >  2:2368887/1‑93 (MQ=255)

                                  cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                      <  1:6618098/93‑1 (MQ=255)

                                  cGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgga                                                      <  2:3597020/93‑1 (MQ=255)

                                   gTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatggat                                                     >  2:2411761/1‑93 (MQ=255)

                                      gCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTc                                                  <  1:6054940/93‑1 (MQ=255)

                                       ccTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTcc                                                 <  1:2327856/93‑1 (MQ=255)

                                           cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTa                                             <  1:5010365/93‑1 (MQ=255)

                                            tGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc                                            >  2:5989536/1‑93 (MQ=255)

                                            tGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAc                                            <  1:1465402/93‑1 (MQ=255)

                                             gCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACg                                           >  2:7454487/1‑93 (MQ=255)

                                             gCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACg                                           <  2:1527827/93‑1 (MQ=255)

                                             gCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACg                                           <  2:6606284/93‑1 (MQ=255)

                                               tACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa                                         >  1:4275933/1‑93 (MQ=255)

                                                aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAt                                        >  1:3195739/1‑93 (MQ=255)

                                                  aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                      <  1:374242/93‑1 (MQ=255)

                                                  aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATaa                                      <  1:970766/93‑1 (MQ=255)

                                                   ttACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc                                     >  1:1694727/1‑93 (MQ=255)

                                                   ttACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAc                                     >  1:587619/1‑93 (MQ=255)

                                                    tACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACt                                    <  2:4735270/93‑1 (MQ=255)

                                                     aCCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTg                                   >  2:5300280/1‑93 (MQ=255)

                                                      ccGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg                                  >  2:3345251/1‑93 (MQ=255)

                                                       cGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGa                                 <  2:3890023/93‑1 (MQ=255)

                                                        ggCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa                                <  1:787473/93‑1 (MQ=255)

                                                        ggCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa                                >  2:4963464/1‑93 (MQ=255)

                                                        ggCCTGCACCAGCACCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa                                <  2:1239550/93‑1 (MQ=255)

                                                         gCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAAc                               <  1:3499128/93‑1 (MQ=255)

                                                          ccTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa                              <  2:2044207/93‑1 (MQ=255)

                                                          ccTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa                              <  1:1281975/93‑1 (MQ=255)

                                                          ccTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa                              <  2:7817590/93‑1 (MQ=255)

                                                          ccTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa                              <  1:5531870/93‑1 (MQ=255)

                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                            <  1:980166/93‑1 (MQ=255)

                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                            >  1:2494034/1‑93 (MQ=255)

                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                            <  1:3721232/93‑1 (MQ=255)

                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                            <  2:2516944/93‑1 (MQ=255)

                                                            tGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                            <  2:521796/93‑1 (MQ=255)

                                                            cgCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                            <  1:7712235/92‑1 (MQ=255)

                                                            cgCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATa                            <  1:3436523/92‑1 (MQ=255)

                                                             gCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAg                           >  1:6466244/1‑93 (MQ=255)

                                                             gCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAg                           >  2:6304093/1‑93 (MQ=255)

                                                             gCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAg                           <  1:3153639/93‑1 (MQ=255)

                                                               aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                         <  2:3436134/93‑1 (MQ=255)

                                                               aCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGtt                         >  2:4632541/1‑93 (MQ=255)

                                                                ccAGCTCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                        <  2:7614494/93‑1 (MQ=255)

                                                                ccAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTtc                        >  2:3579953/1‑93 (MQ=255)

                                                                   gcgcCGCAGCGACCTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                     <  2:6854143/93‑1 (MQ=255)

                                                                   gcgcCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                     >  1:6271854/1‑93 (MQ=255)

                                                                   gcgcCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTctcc                     >  1:1321617/1‑93 (MQ=255)

                                                                      ccgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctct                  >  2:1330454/1‑93 (MQ=255)

                                                                       cgcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctc                 >  1:1066094/1‑93 (MQ=255)

                                                                        gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTGCGGATAACTGGAACATAGTTCTCctctct                <  1:467861/93‑1 (MQ=255)

                                                                        gcAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCctctct                >  2:2265213/1‑93 (MQ=255)

                                                                         cAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCtt               >  2:6203663/1‑93 (MQ=255)

                                                                         cAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCtt               >  2:1595961/1‑93 (MQ=255)

                                                                          aGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg              >  2:91102/1‑93 (MQ=255)

                                                                          aGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTg              >  1:743991/1‑93 (MQ=255)

                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTTTACGTATAACTGGAACATAGTTCTCCTCTCTTGc             >  1:7032705/1‑93 (MQ=255)

                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc             <  2:1201278/93‑1 (MQ=255)

                                                                           gCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGc             >  1:5623203/1‑93 (MQ=255)

                                                                             gACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTg           <  2:5581154/93‑1 (MQ=255)

                                                                              aCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa          >  2:7574/1‑93 (MQ=255)

                                                                              aCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGa          <  1:5233899/93‑1 (MQ=255)

                                                                               ccTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGaa         <  2:3056264/93‑1 (MQ=255)

                                                                                cTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCGGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAt        <  1:2970311/93‑1 (MQ=255)

                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt       <  2:1864174/93‑1 (MQ=255)

                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt       >  1:5997553/1‑93 (MQ=255)

                                                                                 tGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAAtt       <  2:2706860/93‑1 (MQ=255)

                                                                                  ggCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTg      >  2:7315033/1‑93 (MQ=255)

                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa   >  2:5185624/1‑93 (MQ=255)

                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa   <  2:5895592/93‑1 (MQ=255)

                                                                                     gAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGaaa   <  2:1371603/93‑1 (MQ=255)

                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc  <  1:5098492/93‑1 (MQ=255)

                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc  <  2:5758872/93‑1 (MQ=255)

                                                                                      aaTCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAc  >  1:4748100/1‑93 (MQ=255)

GAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC  >  NC_000913/2261364‑2261542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261361‑2261547

GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCA                                                                                               <  SRR4302543.358674/94‑1 (MQ=60)

GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCA                                                                                               >  SRR4302543.5547116/1‑94 (MQ=60)

 TTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAC                                                                                              >  SRR4302543.6106776/1‑94 (MQ=60)

 TTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCAC                                                                                              >  SRR4302543.2715367/1‑94 (MQ=60)

  TGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACC                                                                                             >  SRR4302543.7596744/1‑94 (MQ=60)

  TGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACC                                                                                             <  SRR4302543.1831862/94‑1 (MQ=60)

      GTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTT                                                                                         >  SRR4302543.5662249/1‑94 (MQ=60)

      GTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTT                                                                                         <  SRR4302543.2530251/94‑1 (MQ=60)

        TTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCT                                                                                       <  SRR4302543.985792/94‑1 (MQ=60)

          GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     <  SRR4302543.3194462/94‑1 (MQ=60)

          GCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTT                                                                                     >  SRR4302543.2853392/1‑94 (MQ=60)

           CTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTTCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTG                                                                                    <  SRR4302543.7009710/94‑1 (MQ=60)

            TGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACGTCTTCTTTGT                                                                                   <  SRR4302543.1803645/94‑1 (MQ=60)

            TGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGT                                                                                   >  SRR4302543.4145006/1‑94 (MQ=60)

            TGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGT                                                                                   >  SRR4302543.6027894/1‑94 (MQ=60)

                CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCC                                                                               >  SRR4302543.5928224/1‑94 (MQ=60)

                CGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCC                                                                               >  SRR4302543.5928226/1‑94 (MQ=60)

                 GCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCG                                                                              <  SRR4302543.1379319/94‑1 (MQ=60)

                 GCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCG                                                                              >  SRR4302543.2067418/1‑94 (MQ=60)

                 GCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCG                                                                              <  SRR4302543.7466455/94‑1 (MQ=60)

                 GCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCG                                                                              >  SRR4302543.6828968/1‑94 (MQ=60)

                  CGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGG                                                                             <  SRR4302543.6181317/94‑1 (MQ=60)

                     GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          >  SRR4302543.7423529/1‑94 (MQ=60)

                     GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          >  SRR4302543.6308203/1‑94 (MQ=60)

                     GCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCT                                                                          >  SRR4302543.6401375/1‑94 (MQ=60)

                        AGCATGNCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTT                                                                       <  SRR4302543.779975/94‑1 (MQ=60)

                        AGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTT                                                                       >  SRR4302543.5547533/1‑94 (MQ=60)

                        AGCATGCCATTGACGTAGCCTTCTGCTACATTACCCGCCTGCACCAGCGCCGCAGCGCCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTT                                                                       >  SRR4302543.5547534/1‑94 (MQ=60)

                           TTGCCATTTACGTAGCCTTCTTCTACATTACCGGTCTGCACCTTCGCCGCAGCGACCTGGCGAATCACCTCTTCTTGGTCTCCGGCCTTTTCGC                                                                    >  SRR4302543.7088180/1‑94 (MQ=60)

                           ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCCCCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302543.3938729/1‑94 (MQ=60)

                           ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302543.7878514/1‑94 (MQ=60)

                           ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302543.7386525/1‑94 (MQ=60)

                           ATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGC                                                                    >  SRR4302543.5462186/1‑94 (MQ=60)

                            TGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCC                                                                   >  SRR4302543.7485639/1‑94 (MQ=60)

                             GCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCC                                                                  <  SRR4302543.4438188/94‑1 (MQ=60)

                             GCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCC                                                                  >  SRR4302543.6881947/1‑94 (MQ=60)

                              CCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCG                                                                 <  SRR4302543.4211904/94‑1 (MQ=60)

                                ATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGA                                                               >  SRR4302543.4272567/1‑94 (MQ=60)

                                 TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302543.7558377/94‑1 (MQ=60)

                                 TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              >  SRR4302543.6522833/1‑94 (MQ=60)

                                 TTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGAT                                                              <  SRR4302543.5664405/94‑1 (MQ=60)

                                    ACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGA                                                           >  SRR4302543.6721062/1‑94 (MQ=60)

                                     CGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGAT                                                          <  SRR4302543.6680501/94‑1 (MQ=60)

                                       TAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGT                                                        <  SRR4302543.1246673/94‑1 (MQ=60)

                                        AGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTC                                                       >  SRR4302543.5185248/1‑94 (MQ=60)

                                         GCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCC                                                      >  SRR4302543.3758690/1‑94 (MQ=60)

                                         GCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCC                                                      <  SRR4302543.6112261/94‑1 (MQ=60)

                                         GACTTCTGCTACATTACCGGCGTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCC                                                      <  SRR4302543.3874061/94‑1 (MQ=60)

                                          CCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     <  SRR4302543.5602800/94‑1 (MQ=60)

                                          CCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCT                                                     <  SRR4302543.2348997/94‑1 (MQ=60)

                                           CTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTG                                                    >  SRR4302543.2974864/1‑94 (MQ=60)

                                             TCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTA                                                  >  SRR4302543.5091985/1‑94 (MQ=60)

                                              CTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAC                                                 >  SRR4302543.5289485/1‑94 (MQ=60)

                                              CTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTAC                                                 <  SRR4302543.5055964/94‑1 (MQ=60)

                                               TGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACG                                                <  SRR4302543.1478606/94‑1 (MQ=60)

                                               TGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACG                                                <  SRR4302543.3624567/94‑1 (MQ=60)

                                                GCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGG                                               >  SRR4302543.4973929/1‑94 (MQ=60)

                                                GCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGG                                               <  SRR4302543.96836/94‑1 (MQ=60)

                                                GCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGG                                               <  SRR4302543.6335202/94‑1 (MQ=60)

                                                 CTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGA                                              >  SRR4302543.1613186/1‑94 (MQ=60)

                                                 CTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGA                                              >  SRR4302543.3926127/1‑94 (MQ=60)

                                                 CTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGA                                              >  SRR4302543.4314796/1‑94 (MQ=60)

                                                  TACATTACCGGCCTGCACCAGCGCCGCCGCGACCTGGCGCATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             >  SRR4302543.3224551/1‑94 (MQ=60)

                                                  TACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAT                                             >  SRR4302543.3224552/1‑94 (MQ=60)

                                                    CATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAA                                           >  SRR4302543.3685574/1‑94 (MQ=60)

                                                     ATTCCCGGCCTGCACCAGCCCCGCAGCGCCCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          <  SRR4302543.6328521/94‑1 (MQ=60)

                                                     ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          <  SRR4302543.979303/94‑1 (MQ=60)

                                                     ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          >  SRR4302543.7264152/1‑94 (MQ=60)

                                                     ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          >  SRR4302543.592988/1‑94 (MQ=60)

                                                     ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          <  SRR4302543.377687/94‑1 (MQ=60)

                                                     ATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAAC                                          >  SRR4302543.1709927/1‑94 (MQ=60)

                                                      TTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACT                                         <  SRR4302543.1415498/94‑1 (MQ=60)

                                                          CGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAA                                     <  SRR4302543.662744/94‑1 (MQ=60)

                                                           GGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAAC                                    <  SRR4302543.794401/94‑1 (MQ=60)

                                                           GGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAAC                                    >  SRR4302543.5579417/1‑94 (MQ=60)

                                                            GCCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACA                                   <  SRR4302543.3530428/94‑1 (MQ=60)

                                                             CCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT                                  <  SRR4302543.1293408/94‑1 (MQ=60)

                                                             CCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT                                  <  SRR4302543.5582134/94‑1 (MQ=60)

                                                             CCTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT                                  >  SRR4302543.2728857/1‑94 (MQ=60)

                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 <  SRR4302543.6077553/94‑1 (MQ=60)

                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 >  SRR4302543.2516655/1‑94 (MQ=60)

                                                              CTGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATA                                 >  SRR4302543.5108246/1‑94 (MQ=60)

                                                               TGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                <  SRR4302543.988819/94‑1 (MQ=60)

                                                               TGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                <  SRR4302543.3753959/94‑1 (MQ=60)

                                                               TGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                >  SRR4302543.6527165/1‑94 (MQ=60)

                                                               CGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                <  SRR4302543.3467281/94‑1 (MQ=60)

                                                               CGCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAG                                <  SRR4302543.7785183/94‑1 (MQ=60)

                                                                GCACCAGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGT                               <  SRR4302543.3182017/94‑1 (MQ=60)

                                                                     AGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCC                          >  SRR4302543.6330987/1‑94 (MQ=60)

                                                                     AGCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCC                          >  SRR4302543.1333360/1‑94 (MQ=60)

                                                                      GCGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCT                         <  SRR4302543.2418185/94‑1 (MQ=60)

                                                                       CGCCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTC                        >  SRR4302543.6469186/1‑94 (MQ=60)

                                                                         CCGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTC                      >  SRR4302543.1075675/1‑94 (MQ=60)

                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     >  SRR4302543.5645541/1‑94 (MQ=60)

                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     <  SRR4302543.2262581/94‑1 (MQ=60)

                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTAT                     >  SRR4302543.7514717/1‑94 (MQ=60)

                                                                          CGCAGCGACCTGGCGAATCACCTCTTCTTTGTCGCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCT                     <  SRR4302543.2262578/94‑1 (MQ=60)

                                                                           GCAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTGCGGATAACTGGAACATAGTTCTCCTCTCTT                    <  SRR4302543.472209/94‑1 (MQ=60)

                                                                            CAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTG                   >  SRR4302543.750613/1‑94 (MQ=60)

                                                                            CAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTG                   >  SRR4302543.3327768/1‑94 (MQ=60)

                                                                            CAGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTG                   <  SRR4302543.7596963/94‑1 (MQ=60)

                                                                             AGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTTTACGTATAACTGGAACATAGTTCTCCTCTCTTGC                  >  SRR4302543.7100340/1‑94 (MQ=60)

                                                                             AGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGC                  >  SRR4302543.5674449/1‑94 (MQ=60)

                                                                             AGCGACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGC                  >  SRR4302543.7450368/1‑94 (MQ=60)

                                                                                GACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGA               >  SRR4302543.6976318/1‑94 (MQ=60)

                                                                                GACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGA               >  SRR4302543.3045013/1‑94 (MQ=60)

                                                                                 ACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAA              <  SRR4302543.1306639/94‑1 (MQ=60)

                                                                                 ACCTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAA              <  SRR4302543.5281056/94‑1 (MQ=60)

                                                                                   CTGGCGAATCCCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCGGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            <  SRR4302543.2997284/94‑1 (MQ=60)

                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            >  SRR4302543.6052590/1‑94 (MQ=60)

                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            >  SRR4302543.6187161/1‑94 (MQ=60)

                                                                                   CTGGCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATT            >  SRR4302543.4761005/1‑94 (MQ=60)

                                                                                      GCGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGCATGTCCTCTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAA         <  SRR4302543.5304337/94‑1 (MQ=60)

                                                                                       CGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAA        >  SRR4302543.3495170/1‑94 (MQ=60)

                                                                                       CGAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAA        <  SRR4302543.4713942/94‑1 (MQ=60)

                                                                                        GAATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAAC       >  SRR4302543.4791496/1‑94 (MQ=60)

                                                                                         AATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACG      <  SRR4302543.5144656/94‑1 (MQ=60)

                                                                                          ATCACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGA     >  SRR4302543.2204257/1‑94 (MQ=60)

                                                                                            CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   >  SRR4302543.7064467/1‑94 (MQ=60)

                                                                                            CACCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATT   >  SRR4302543.6548926/1‑94 (MQ=60)

                                                                                             CCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  <  SRR4302543.5195187/94‑1 (MQ=60)

                                                                                             ACCTCTTCTTTGTCTCCGGCCTTGTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  <  SRR4302543.5195186/94‑1 (MQ=60)

GTTGAGGTTTGCTGTTCGCGCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACATAGTTCTCCTCTCTTGCTGAATTGAAACGATTC  >  NC_000913/2261361‑2261547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`