Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,933,024	T→C	R256R (CGT→CGC)	fucP →	fucose permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,933,024	0	T	C	99.6%	744.5 / NA	233	R256R (CGT→CGC)	fucP	fucose permease
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (115/117); minor base A (1/0); total (116/117)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 245 total aligned reads displayed.

CATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGTATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGT  >  NC_000913/2932934‑2933113

cATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAt                                                                                         <  1:4348020/93‑1 (MQ=255)

cATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAt                                                                                         <  2:2419428/93‑1 (MQ=255)

 aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                        <  2:573318/93‑1 (MQ=255)

 aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                        >  2:138692/1‑93 (MQ=255)

  tGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTc                                                                                       <  2:804737/93‑1 (MQ=255)

  tGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTc                                                                                       >  1:3371238/1‑93 (MQ=255)

    cTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGc                                                                                     >  2:3101859/1‑93 (MQ=255)

     tGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCNCATTCGcc                                                                                    >  2:3284064/1‑93 (MQ=255)

     tGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGcc                                                                                    >  1:1185169/1‑93 (MQ=255)

      gACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCa                                                                                   >  1:4621401/1‑93 (MQ=255)

      gACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCa                                                                                   >  1:1992076/1‑93 (MQ=255)

       aCGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCAc                                                                                  <  2:2012503/93‑1 (MQ=255)

        cGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACt                                                                                 <  2:3327076/93‑1 (MQ=255)

         gAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTg                                                                                >  2:1924113/1‑93 (MQ=255)

          aaaTTCCCGGCATTGCAGAGTGATAATCACAGTGGCGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTgg                                                                               <  1:527937/93‑1 (MQ=255)

          aaaTTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTgg                                                                               >  2:3243094/1‑93 (MQ=255)

           aaTTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGgc                                                                              <  2:4314684/93‑1 (MQ=255)

             ttCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGgcgc                                                                            >  1:3086276/1‑93 (MQ=255)

              tCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCt                                                                           >  2:4685784/1‑93 (MQ=255)

                ccGGCATTGCAGAGTGATAATCACAGTGACGCCAATCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTgg                                                                         <  2:3725466/93‑1 (MQ=255)

                ccGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTgg                                                                         >  2:902095/1‑93 (MQ=255)

                ccGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTgg                                                                         >  1:1936292/1‑93 (MQ=255)

                  ggCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGc                                                                       >  2:1847604/1‑93 (MQ=255)

                  ggCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGc                                                                       >  2:1936614/1‑93 (MQ=255)

                  ggCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGc                                                                       <  2:3191265/93‑1 (MQ=255)

                   gCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCg                                                                      >  2:2698690/1‑93 (MQ=255)

                      ttGCAGAGTGATAATCCCAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTa                                                                   <  2:4378816/93‑1 (MQ=255)

                      ttGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTa                                                                   >  1:4312427/1‑93 (MQ=255)

                         cAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTa                                                                <  1:2792519/93‑1 (MQ=255)

                          agagTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAg                                                               <  2:299909/93‑1 (MQ=255)

                           gagTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAgc                                                              >  1:2018154/1‑93 (MQ=255)

                           gagTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAgc                                                              <  2:2552833/93‑1 (MQ=255)

                               gATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCaa                                                          <  2:3430438/93‑1 (MQ=255)

                               gATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCaa                                                          <  1:4235371/93‑1 (MQ=255)

                                aTAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAt                                                         >  2:3834775/1‑93 (MQ=255)

                                aTAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAt                                                         >  1:1217340/1‑93 (MQ=255)

                                 tAATCACAGTGACGCCAAACAAGGATCGGTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAtt                                                        >  1:2489578/1‑93 (MQ=255)

                                     cacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGc                                                    <  1:1051517/93‑1 (MQ=255)

                                     cacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGc                                                    >  2:305844/1‑93 (MQ=255)

                                     cacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGc                                                    >  1:2392062/1‑93 (MQ=255)

                                     cacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGc                                                    <  1:2307108/93‑1 (MQ=255)

                                      acaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCt                                                   >  2:693842/1‑93 (MQ=255)

                                       caGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTa                                                  <  2:2792383/93‑1 (MQ=255)

                                       caGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTa                                                  <  2:1657000/93‑1 (MQ=255)

                                       caGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCATGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTa                                                  <  1:2686089/93‑1 (MQ=255)

                                          tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               <  2:3131783/93‑1 (MQ=255)

                                          tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               >  1:4390318/1‑93 (MQ=255)

                                          tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               <  2:4152162/93‑1 (MQ=255)

                                          tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               <  2:2239052/93‑1 (MQ=255)

                                          tGACACCAAACAAGGATCGTTCTCCTCATCGCTTTCGCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                               <  1:3920818/93‑1 (MQ=255)

                                            aCGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCg                                             >  1:1663332/1‑93 (MQ=255)

                                             cGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCgg                                            <  1:3440802/93‑1 (MQ=255)

                                                cAAACAAGGATCGTTCTCCACATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGgcgc                                         >  2:4526465/1‑93 (MQ=255)

                                                  aaCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGcac                                       >  2:2157865/1‑93 (MQ=255)

                                                  aaCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGcac                                       >  1:3146566/1‑93 (MQ=255)

                                                   aCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGcaca                                      >  1:2098770/1‑93 (MQ=255)

                                                   aCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGcaca                                      >  2:28182/1‑93 (MQ=255)

                                                    cAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACaa                                     >  2:2712643/1‑93 (MQ=255)

                                                     aaGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACaaa                                    <  2:1530843/93‑1 (MQ=255)

                                                     aaGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACaaa                                    <  1:79841/93‑1 (MQ=255)

                                                      aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                   >  1:3039372/1‑93 (MQ=255)

                                                      aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                   <  2:4621401/93‑1 (MQ=255)

                                                      aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                   <  1:457737/93‑1 (MQ=255)

                                                      aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCACCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                   >  1:1761165/1‑93 (MQ=255)

                                                         aTCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGc                                <  2:2895825/93‑1 (MQ=255)

                                                         aTCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGc                                <  1:104087/93‑1 (MQ=255)

                                                           cGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              <  1:1139594/93‑1 (MQ=255)

                                                           cGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              >  1:4433342/1‑93 (MQ=255)

                                                           cGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              >  1:159237/1‑93 (MQ=255)

                                                           cGTTCTCCGCATCGCTGTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              <  1:888975/93‑1 (MQ=255)

                                                           cGTGCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCct                              <  2:3894640/93‑1 (MQ=255)

                                                            gTTCTCCGCATCGCTTTCTCGCCTGGCGCGAATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCctg                             >  2:2459632/1‑93 (MQ=255)

                                                              tctcCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACACACGGCctgct                           >  2:448769/1‑93 (MQ=255)

                                                                tcCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTgg                         <  1:3605809/93‑1 (MQ=255)

                                                                tcCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTgg                         >  1:1369554/1‑93 (MQ=255)

                                                                  ctcgtCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAg                       <  1:2933899/89‑1 (MQ=255)

                                                                   gCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGc                      >  1:1797213/1‑93 (MQ=255)

                                                                   gCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGc                      <  2:148117/93‑1 (MQ=255)

                                                                      tCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAt                   >  2:1200020/1‑93 (MQ=255)

                                                                       cGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAtt                  <  1:3966087/93‑1 (MQ=255)

                                                                       cGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAtt                  <  1:13004/93‑1 (MQ=255)

                                                                       cGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAtt                  <  2:2315863/93‑1 (MQ=255)

                                                                        gCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAttt                 >  2:350044/1‑93 (MQ=255)

                                                                        gCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAttt                 <  1:4167810/93‑1 (MQ=255)

                                                                          tttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGa               <  2:2437199/93‑1 (MQ=255)

                                                                          tttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGa               >  1:3211896/1‑93 (MQ=255)

                                                                          tttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGa               >  1:3704782/1‑93 (MQ=255)

                                                                           ttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGAt              <  2:3608969/93‑1 (MQ=255)

                                                                           ttCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGAt              <  2:4012902/93‑1 (MQ=255)

                                                                             ctcGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTc            <  1:4520413/93‑1 (MQ=255)

                                                                                gCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCt         >  1:3822749/1‑93 (MQ=255)

                                                                                gCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCt         >  2:4089476/1‑93 (MQ=255)

                                                                                 ccTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTa        <  2:1796097/93‑1 (MQ=255)

                                                                                 ccTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTa        <  2:207105/93‑1 (MQ=255)

                                                                                  cTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTAc       <  1:242741/93‑1 (MQ=255)

                                                                                  cTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTAc       >  2:3946816/1‑93 (MQ=255)

                                                                                   tGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACg      >  1:1313335/1‑93 (MQ=255)

                                                                                    ggCGCGCATTCGCCGCTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGc     <  2:1104545/93‑1 (MQ=255)

                                                                                    ggCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCGCAAACGGCCTGCTGGAGCTATTTGATTCGCTACGc     <  2:602434/93‑1 (MQ=255)

                                                                                      cgcgCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTg   <  2:3720603/93‑1 (MQ=255)

CATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGTATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGT  >  NC_000913/2932934‑2933113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGTATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTAGAA  >  NC_000913/2932933‑2933117

TCATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAT                                                                                             >  SRR4302535.980792/1‑94 (MQ=60)

 CATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATT                                                                                            <  SRR4302535.4377766/94‑1 (MQ=60)

  ATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTC                                                                                           >  SRR4302535.3394692/1‑94 (MQ=60)

     CTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCC                                                                                        >  SRR4302535.1193637/1‑94 (MQ=60)

      TGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCC                                                                                       >  SRR4302535.4652798/1‑94 (MQ=60)

      TGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCA                                                                                       >  SRR4302535.2006418/1‑94 (MQ=60)

        ACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACT                                                                                     <  SRR4302535.3474995/94‑1 (MQ=60)

         CGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTG                                                                                    >  SRR4302535.1177303/1‑94 (MQ=60)

           AAATTCCCGGCATTGCAGAGTGATAATCACAGTGGCGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGC                                                                                  <  SRR4302535.531624/94‑1 (MQ=60)

           AAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGC                                                                                  <  SRR4302535.818438/94‑1 (MQ=60)

             ATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGC                                                                                >  SRR4302535.3107770/1‑94 (MQ=60)

              TTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCT                                                                               <  SRR4302535.1643147/94‑1 (MQ=60)

               TCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTG                                                                              >  SRR4302535.4685014/1‑94 (MQ=60)

                CCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGG                                                                             >  SRR4302535.2495566/1‑94 (MQ=60)

                CCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGG                                                                             >  SRR4302535.1950237/1‑94 (MQ=60)

                 CCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGG                                                                            <  SRR4302535.1280870/94‑1 (MQ=60)

                 CCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGG                                                                            >  SRR4302535.3102216/1‑94 (MQ=60)

                   GGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCG                                                                          <  SRR4302535.1814634/94‑1 (MQ=60)

                     CATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGT                                                                        >  SRR4302535.2582405/1‑94 (MQ=60)

                      ATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTA                                                                       >  SRR4302535.4341927/1‑94 (MQ=60)

                        TGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATT                                                                     <  SRR4302535.1181430/94‑1 (MQ=60)

                        TGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATT                                                                     <  SRR4302535.1383801/94‑1 (MQ=60)

                          CAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAG                                                                   >  SRR4302535.3946267/1‑94 (MQ=60)

                          CAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAG                                                                   <  SRR4302535.1056797/94‑1 (MQ=60)

                          CAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAG                                                                   >  SRR4302535.3417178/1‑94 (MQ=60)

                          CAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAG                                                                   <  SRR4302535.2812391/94‑1 (MQ=60)

                           AGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGC                                                                  >  SRR4302535.3926172/1‑94 (MQ=60)

                           AGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGC                                                                  >  SRR4302535.2032696/1‑94 (MQ=60)

                            GANTNANANACACAGTGACGNNNAACANGGATCGTTCTCCGCNTCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCG                                                                 <  SRR4302535.1717826/94‑1 (MQ=60)

                             AGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGC                                                                >  SRR4302535.4351802/1‑94 (MQ=60)

                               TGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAA                                                              >  SRR4302535.805275/1‑94 (MQ=60)

                                GATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAT                                                             >  SRR4302535.3986004/1‑94 (MQ=60)

                                GATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAT                                                             <  SRR4302535.4264359/94‑1 (MQ=60)

                                GATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAT                                                             >  SRR4302535.1226072/1‑94 (MQ=60)

                                 ATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATT                                                            <  SRR4302535.4417545/94‑1 (MQ=60)

                                 ATAATCACAGTGACGCCAAACAAGGATCGGTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATT                                                            >  SRR4302535.2507532/1‑94 (MQ=60)

                                     TCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGC                                                        >  SRR4302535.2409260/1‑94 (MQ=60)

                                     TCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGC                                                        <  SRR4302535.2095221/94‑1 (MQ=60)

                                      CACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCT                                                       <  SRR4302535.1058995/94‑1 (MQ=60)

                                      CACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCT                                                       <  SRR4302535.2323610/94‑1 (MQ=60)

                                      CACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCT                                                       <  SRR4302535.4645624/94‑1 (MQ=60)

                                        CAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTAT                                                     <  SRR4302535.4552065/94‑1 (MQ=60)

                                        CAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCATGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTAT                                                     <  SRR4302535.2705293/94‑1 (MQ=60)

                                          GTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGT                                                   >  SRR4302535.4420357/1‑94 (MQ=60)

                                           TGACACCAAACAAGGATCGTTCTCCTCATCGCTTTCGCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTC                                                  <  SRR4302535.3947625/94‑1 (MQ=60)

                                            GACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCG                                                 >  SRR4302535.1675349/1‑94 (MQ=60)

                                              CGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGC                                               >  SRR4302535.3722576/1‑94 (MQ=60)

                                              CGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGC                                               <  SRR4302535.3464730/94‑1 (MQ=60)

                                                 CAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCA                                            >  SRR4302535.3132023/1‑94 (MQ=60)

                                                  AAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCAC                                           >  SRR4302535.4230551/1‑94 (MQ=60)

                                                  AAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCAC                                           >  SRR4302535.3168445/1‑94 (MQ=60)

                                                   AACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACA                                          <  SRR4302535.741151/94‑1 (MQ=60)

                                                   AACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACA                                          >  SRR4302535.2113908/1‑94 (MQ=60)

                                                   AACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACA                                          >  SRR4302535.3576867/1‑94 (MQ=60)

                                                   AACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACA                                          >  SRR4302535.4120286/1‑94 (MQ=60)

                                                   AACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACA                                          <  SRR4302535.1347263/94‑1 (MQ=60)

                                                      AAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAC                                       <  SRR4302535.80410/94‑1 (MQ=60)

                                                      AAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAC                                       <  SRR4302535.1779189/94‑1 (MQ=60)

                                                      AAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAC                                       <  SRR4302535.3382092/94‑1 (MQ=60)

                                                      AAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAC                                       >  SRR4302535.3060659/1‑94 (MQ=60)

                                                      AAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCACCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAC                                       >  SRR4302535.1773919/1‑94 (MQ=60)

                                                       AGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACG                                      <  SRR4302535.4099500/94‑1 (MQ=60)

                                                       AGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACG                                      <  SRR4302535.136007/94‑1 (MQ=60)

                                                       AGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACG                                      <  SRR4302535.2023288/94‑1 (MQ=60)

                                                       AGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACG                                      <  SRR4302535.460937/94‑1 (MQ=60)

                                                        AGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGG                                     <  SRR4302535.300117/94‑1 (MQ=60)

                                                         GATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGC                                    >  SRR4302535.2935761/1‑94 (MQ=60)

                                                          ATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCC                                   <  SRR4302535.104827/94‑1 (MQ=60)

                                                           TCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCT                                  >  SRR4302535.4463720/1‑94 (MQ=60)

                                                           TCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCT                                  >  SRR4302535.160354/1‑94 (MQ=60)

                                                            TGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTG                                 <  SRR4302535.3302351/94‑1 (MQ=60)

                                                            CGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTG                                 <  SRR4302535.1147715/94‑1 (MQ=60)

                                                            CGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTG                                 <  SRR4302535.4469839/94‑1 (MQ=60)

                                                            CGTTCTCCGCATCGCTGTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTG                                 <  SRR4302535.895274/94‑1 (MQ=60)

                                                              TTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCT                               <  SRR4302535.1653296/94‑1 (MQ=60)

                                                               TCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTG                              <  SRR4302535.2026246/94‑1 (MQ=60)

                                                                CTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGG                             >  SRR4302535.1379341/1‑94 (MQ=60)

                                                                CTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGG                             >  SRR4302535.1077829/1‑94 (MQ=60)

                                                                 TCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGA                            <  SRR4302535.3630823/94‑1 (MQ=60)

                                                                   ctcgTCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGC                          <  SRR4302535.2954547/90‑1 (MQ=60)

                                                                   CGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGC                          >  SRR4302535.1810206/1‑94 (MQ=60)

                                                                   CGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGC                          <  SRR4302535.4514633/94‑1 (MQ=60)

                                                                      ATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAT                       >  SRR4302535.131863/1‑94 (MQ=60)

                                                                      ATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAT                       >  SRR4302535.2186152/1‑94 (MQ=60)

                                                                       TCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATT                      <  SRR4302535.2689537/94‑1 (MQ=60)

                                                                        CGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTT                     <  SRR4302535.13103/94‑1 (MQ=60)

                                                                        CGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTT                     >  SRR4302535.1914232/1‑94 (MQ=60)

                                                                        CGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTT                     <  SRR4302535.3993178/94‑1 (MQ=60)

                                                                         GCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTG                    <  SRR4302535.4196348/94‑1 (MQ=60)

                                                                         GCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTG                    >  SRR4302535.3170218/1‑94 (MQ=60)

                                                                          CTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGA                   >  SRR4302535.3234140/1‑94 (MQ=60)

                                                                          CTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGA                   >  SRR4302535.1711133/1‑94 (MQ=60)

                                                                          CTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGA                   >  SRR4302535.3730441/1‑94 (MQ=60)

                                                                          CTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGA                   >  SRR4302535.4385184/1‑94 (MQ=60)

                                                                          CTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGA                   >  SRR4302535.1249291/1‑94 (MQ=60)

                                                                           TTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGAT                  >  SRR4302535.244365/1‑94 (MQ=60)

                                                                           TTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGAT                  >  SRR4302535.3143512/1‑94 (MQ=60)

                                                                           TTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGAT                  >  SRR4302535.2419615/1‑94 (MQ=60)

                                                                            TTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATT                 <  SRR4302535.1970086/94‑1 (MQ=60)

                                                                             TCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTC                <  SRR4302535.3813230/94‑1 (MQ=60)

                                                                              CTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCG               <  SRR4302535.4551394/94‑1 (MQ=60)

                                                                              CTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCG               <  SRR4302535.4295282/94‑1 (MQ=60)

                                                                              CTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCG               <  SRR4302535.4443571/94‑1 (MQ=60)

                                                                                CGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCT             >  SRR4302535.3849118/1‑94 (MQ=60)

                                                                                 GCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTA            <  SRR4302535.4672941/94‑1 (MQ=60)

                                                                                  CCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTTTTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTAC           >  SRR4302535.2214900/1‑94 (MQ=60)

                                                                                  CCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTAC           >  SRR4302535.2800975/1‑94 (MQ=60)

                                                                                  CCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTAC           >  SRR4302535.4339678/1‑94 (MQ=60)

                                                                                   CTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACG          <  SRR4302535.244403/94‑1 (MQ=60)

                                                                                   CTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACG          >  SRR4302535.1322723/1‑94 (MQ=60)

                                                                                     GGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCT        <  SRR4302535.2958439/94‑1 (MQ=60)

                                                                                      GCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTG       <  SRR4302535.1221438/94‑1 (MQ=60)

                                                                                       CGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGT      >  SRR4302535.2349420/1‑94 (MQ=60)

                                                                                        GCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTA     >  SRR4302535.2366506/1‑94 (MQ=60)

                                                                                         CGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTAG    >  SRR4302535.2259328/1‑94 (MQ=60)

                                                                                          GCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTAGA   <  SRR4302535.47009/94‑1 (MQ=60)

                                                                                           CATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTAGAA  >  SRR4302535.718547/1‑94 (MQ=60)

                                                                                           CATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTAGAA  >  SRR4302535.3749455/1‑94 (MQ=60)

                                                                                           CATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTAGAA  >  SRR4302535.3786293/1‑94 (MQ=60)

                                                                                           CATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTAGAA  >  SRR4302535.2469301/1‑94 (MQ=60)

                                                                                           CATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTAGGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTAGAA  <  SRR4302535.1199064/94‑1 (MQ=60)

TCATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGTATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTAGAA  >  NC_000913/2932933‑2933117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`