Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,940,324 G→A A89V (GCC→GTC)  gcvA ← GcvA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,940,3240GA100.0% 633.4 / NA 180A89V (GCC→GTC) gcvAGcvA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (69/111);  total (69/111)

Only 100 of 186 total aligned reads displayed.

GCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAG  >  NC_000913/2940234‑2940412
                                                                                          |                                                                                        
gCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGAcc                                                                                        <  1:4319618/93‑1 (MQ=255)
gCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGAcc                                                                                        <  2:2899429/93‑1 (MQ=255)
gCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGAcc                                                                                        <  2:2992262/93‑1 (MQ=255)
   gAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTgg                                                                                     <  1:2695700/93‑1 (MQ=255)
       tAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGtt                                                                                 <  2:3896347/93‑1 (MQ=255)
          aGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTAc                                                                              <  1:4187022/93‑1 (MQ=255)
           gCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTAcg                                                                             >  1:1033276/1‑93 (MQ=255)
           gCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTAcg                                                                             >  1:284597/1‑93 (MQ=255)
             tGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTAcgcg                                                                           >  1:4543097/1‑93 (MQ=255)
             tGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTAcgcg                                                                           >  1:3762634/1‑93 (MQ=255)
              ggAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGt                                                                          <  1:1292610/93‑1 (MQ=255)
               gAAAGTCGCGGAACCAACCAAAGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCATGGCGCTACGGACCTGGAGTTTACGCGTc                                                                         >  2:1277397/1‑93 (MQ=255)
                aaaGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCg                                                                        <  2:4503171/93‑1 (MQ=255)
                 aaGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGc                                                                       >  1:1656548/1‑93 (MQ=255)
                 aaGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGc                                                                       <  2:2132179/93‑1 (MQ=255)
                  aGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCt                                                                      >  1:1863259/1‑93 (MQ=255)
                   gTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCtt                                                                     <  1:1024712/93‑1 (MQ=255)
                   gTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCtt                                                                     <  2:4667194/93‑1 (MQ=255)
                    tCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTc                                                                    <  2:3484247/93‑1 (MQ=255)
                    tCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTc                                                                    >  1:1762079/1‑93 (MQ=255)
                     cgcgGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCg                                                                   <  1:982385/93‑1 (MQ=255)
                        ggAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGtt                                                                <  2:4397628/93‑1 (MQ=255)
                         gAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTa                                                               >  1:824891/1‑93 (MQ=255)
                         gAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTa                                                               <  1:2398361/93‑1 (MQ=255)
                          aaccaaccAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTaa                                                              <  1:1547643/93‑1 (MQ=255)
                           accaaccaATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAAt                                                             <  1:135952/93‑1 (MQ=255)
                           accaaccaATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAAt                                                             >  1:542897/1‑93 (MQ=255)
                            ccaaccaaTGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAAtt                                                            <  2:532588/93‑1 (MQ=255)
                            ccaaccaaTGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAAtt                                                            <  1:951655/93‑1 (MQ=255)
                            ccaaccaaTGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAAtt                                                            <  2:3308639/93‑1 (MQ=255)
                              aaccaaTGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGc                                                          >  2:1474/1‑93 (MQ=255)
                              aaccaaTGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGCCGCTTCGGTTAATTGc                                                          >  2:2482188/1‑93 (MQ=255)
                                 caaTGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGaa                                                       >  2:272142/1‑93 (MQ=255)
                                  aatgaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGaaa                                                      >  1:896883/1‑93 (MQ=255)
                                   atgaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGaaaa                                                     <  2:926759/93‑1 (MQ=255)
                                   atgaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGaaaa                                                     >  2:4676629/1‑93 (MQ=255)
                                    tgaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAat                                                    >  1:3542848/1‑93 (MQ=255)
                                     gaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAata                                                   >  2:3174458/1‑93 (MQ=255)
                                     gaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAata                                                   >  1:2621913/1‑93 (MQ=255)
                                     gaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAata                                                   <  2:4025573/93‑1 (MQ=255)
                                     gaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAata                                                   <  2:4065850/93‑1 (MQ=255)
                                     gaatgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAata                                                   <  2:4118830/93‑1 (MQ=255)
                                        tgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATAtct                                                >  2:2763680/1‑93 (MQ=255)
                                        tgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATAtct                                                >  2:3794582/1‑93 (MQ=255)
                                        tgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATAtct                                                >  2:2328974/1‑93 (MQ=255)
                                        tgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATAtct                                                <  1:1800245/93‑1 (MQ=255)
                                        tgGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATAtct                                                <  1:3417578/93‑1 (MQ=255)
                                         ggCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATAtctc                                               <  2:4327457/93‑1 (MQ=255)
                                           cGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCtt                                             >  2:753382/1‑93 (MQ=255)
                                           cGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCtt                                             <  2:1180920/93‑1 (MQ=255)
                                            gAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCttt                                            <  1:1660645/93‑1 (MQ=255)
                                             aaaCTTGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTg                                           <  1:2991104/93‑1 (MQ=255)
                                             aaaCTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATCGCGAAAATATCTCTTTg                                           <  1:596349/93‑1 (MQ=255)
                                              aaCTGGGGAGTAAACTGACCGTCAACGCCGCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGa                                          <  2:2202015/93‑1 (MQ=255)
                                              aaCTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGa                                          <  2:3963523/93‑1 (MQ=255)
                                              aaCTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGa                                          <  2:2301947/93‑1 (MQ=255)
                                               aCTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGat                                         <  1:4175881/93‑1 (MQ=255)
                                                cTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGata                                        <  1:718970/93‑1 (MQ=255)
                                                cTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGata                                        <  1:291895/93‑1 (MQ=255)
                                                cTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGata                                        >  2:3370441/1‑93 (MQ=255)
                                                 tGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGatat                                       >  1:3445409/1‑93 (MQ=255)
                                                 tGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGatat                                       <  2:266705/93‑1 (MQ=255)
                                                  ggggAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATc                                      <  2:1124241/93‑1 (MQ=255)
                                                  ggggAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATc                                      <  1:4206130/93‑1 (MQ=255)
                                                    ggAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGa                                    >  2:2565174/1‑93 (MQ=255)
                                                     gAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAg                                   <  1:3431812/93‑1 (MQ=255)
                                                       gTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGa                                 <  1:2073215/93‑1 (MQ=255)
                                                         aaaCTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGaaa                               <  2:1948127/93‑1 (MQ=255)
                                                          aaCTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAAt                              >  2:578905/1‑93 (MQ=255)
                                                          aaCTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAAt                              <  2:464500/93‑1 (MQ=255)
                                                           aCTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATa                             >  2:4419493/1‑93 (MQ=255)
                                                           aCTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATa                             <  1:578704/93‑1 (MQ=255)
                                                           aCTGACCGTCAACGCCCCCGTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATa                             <  2:3612812/93‑1 (MQ=255)
                                                            cTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAg                            <  1:4360997/93‑1 (MQ=255)
                                                             tGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGc                           <  1:3085518/93‑1 (MQ=255)
                                                              gACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCt                          <  2:1245207/93‑1 (MQ=255)
                                                              gACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCt                          <  1:362630/93‑1 (MQ=255)
                                                                ccGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCttt                        >  1:276187/1‑93 (MQ=255)
                                                                  gTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGc                      <  2:635304/93‑1 (MQ=255)
                                                                    cAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGccc                    <  2:817120/93‑1 (MQ=255)
                                                                     aaCGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCt                   <  1:4625485/93‑1 (MQ=255)
                                                                     aaCGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCt                   <  1:1515448/93‑1 (MQ=255)
                                                                      aCGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCtt                  >  1:3426596/1‑93 (MQ=255)
                                                                      aCGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGCTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCtt                  >  1:2481130/1‑93 (MQ=255)
                                                                       cgtccccTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTc                 <  2:3686777/90‑1 (MQ=255)
                                                                       cGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTc                 <  2:1799850/93‑1 (MQ=255)
                                                                        gCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTcc                <  2:2238511/93‑1 (MQ=255)
                                                                          ccccTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTc              <  1:2672160/93‑1 (MQ=255)
                                                                          ccccTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTc              <  2:229962/93‑1 (MQ=255)
                                                                           cccTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCg             <  1:3321220/93‑1 (MQ=255)
                                                                            ccTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCgg            >  1:1698358/1‑93 (MQ=255)
                                                                             cTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGt           >  1:1628804/1‑93 (MQ=255)
                                                                              ttGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGTAAATAGCTTTGCCCTTCCTCGGTc          <  2:2277235/93‑1 (MQ=255)
                                                                              ttGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTc          >  1:2257636/1‑93 (MQ=255)
                                                                                 gcgcTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTcagc       <  2:1675816/93‑1 (MQ=255)
                                                                                   gcTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTcagcag     >  1:3837667/1‑93 (MQ=255)
                                                                                    cTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAgg    >  2:3719993/1‑93 (MQ=255)
                                                                                     tgcGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGa   <  2:1967589/91‑1 (MQ=255)
                                                                                     tACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGa   >  1:2013481/1‑93 (MQ=255)
                                                                                      aCGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAg  <  1:763234/93‑1 (MQ=255)
                                                                                          |                                                                                        
GCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAG  >  NC_000913/2940234‑2940412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAAC  >  NC_000913/2940232‑2940417
                                                                                            |                                                                                             
AAGCTGAATTAGAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGAC                                                                                              <  SRR4302535.1536482/94‑1 (MQ=60)
AAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGAC                                                                                              <  SRR4302535.4344869/94‑1 (MQ=60)
  GCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCT                                                                                            <  SRR4302535.4349168/94‑1 (MQ=60)
    TGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGG                                                                                          >  SRR4302535.3350279/1‑94 (MQ=60)
     GAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGCCCTGGG                                                                                         >  SRR4302535.3239801/1‑94 (MQ=60)
     GAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGA                                                                                         <  SRR4302535.2714966/94‑1 (MQ=60)
      AATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAG                                                                                        <  SRR4302535.86275/94‑1 (MQ=60)
           AAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTAC                                                                                   >  SRR4302535.1731019/1‑94 (MQ=60)
            AGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACG                                                                                  >  SRR4302535.1040617/1‑94 (MQ=60)
            AGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACG                                                                                  <  SRR4302535.2699336/94‑1 (MQ=60)
            AGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACG                                                                                  >  SRR4302535.286585/1‑94 (MQ=60)
            AGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACG                                                                                  <  SRR4302535.4215677/94‑1 (MQ=60)
             GCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGC                                                                                 >  SRR4302535.1566016/1‑94 (MQ=60)
              CTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCG                                                                                >  SRR4302535.4574170/1‑94 (MQ=60)
              CTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCG                                                                                >  SRR4302535.3788630/1‑94 (MQ=60)
               TGGAAAGTCGCGGAACCAACCAATGCATGTCGAAACTGAGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGC                                                                               >  SRR4302535.2083957/1‑94 (MQ=60)
               TGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGT                                                                               >  SRR4302535.746194/1‑94 (MQ=60)
                GGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTC                                                                              <  SRR4302535.4607816/94‑1 (MQ=60)
                GGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTC                                                                              >  SRR4302535.471755/1‑94 (MQ=60)
                GGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTC                                                                              <  SRR4302535.1301820/94‑1 (MQ=60)
                  AAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGC                                                                            >  SRR4302535.1668531/1‑94 (MQ=60)
                   AAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCT                                                                           >  SRR4302535.1876693/1‑94 (MQ=60)
                    AGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTT                                                                          >  SRR4302535.2879494/1‑94 (MQ=60)
                     GTNGNGNANCCNACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTC                                                                         <  SRR4302535.1360533/94‑1 (MQ=60)
                     GTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTC                                                                         >  SRR4302535.1774843/1‑94 (MQ=60)
                     GTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTC                                                                         <  SRR4302535.1031991/94‑1 (MQ=60)
                       CGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGG                                                                       <  SRR4302535.989365/94‑1 (MQ=60)
                         CGGAACCAACCAATGGATGGCGAAACTGGGGAGTAAACTGAGCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTT                                                                     <  SRR4302535.1731728/94‑1 (MQ=60)
                         CGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTT                                                                     >  SRR4302535.1903305/1‑94 (MQ=60)
                         CGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTT                                                                     <  SRR4302535.593321/94‑1 (MQ=60)
                         CGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTT                                                                     <  SRR4302535.1798121/94‑1 (MQ=60)
                          GGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTA                                                                    <  SRR4302535.1351439/94‑1 (MQ=60)
                          GGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTA                                                                    >  SRR4302535.830674/1‑94 (MQ=60)
                           GAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAA                                                                   <  SRR4302535.3856100/94‑1 (MQ=60)
                           GAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAA                                                                   <  SRR4302535.2415606/94‑1 (MQ=60)
                           GAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAA                                                                   <  SRR4302535.816638/94‑1 (MQ=60)
                            AACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAAT                                                                  <  SRR4302535.1558790/94‑1 (MQ=60)
                            AACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAAT                                                                  >  SRR4302535.546692/1‑94 (MQ=60)
                             ACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATT                                                                 <  SRR4302535.136900/94‑1 (MQ=60)
                              CCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTG                                                                <  SRR4302535.958402/94‑1 (MQ=60)
                               CAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGC                                                               >  SRR4302535.2511505/1‑94 (MQ=60)
                                AACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCG                                                              >  SRR4302535.2960534/1‑94 (MQ=60)
                                AACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCG                                                              <  SRR4302535.3443233/94‑1 (MQ=60)
                                 ACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGA                                                             >  SRR4302535.2385591/1‑94 (MQ=60)
                                  CCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAA                                                            >  SRR4302535.2452697/1‑94 (MQ=60)
                                   CAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAA                                                           >  SRR4302535.903242/1‑94 (MQ=60)
                                   CAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAA                                                           >  SRR4302535.1544261/1‑94 (MQ=60)
                                   CAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAA                                                           >  SRR4302535.3926739/1‑94 (MQ=60)
                                    AATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAA                                                          >  SRR4302535.4357714/1‑94 (MQ=60)
                                     ATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAAT                                                         >  SRR4302535.960431/1‑94 (MQ=60)
                                     ATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAAT                                                         <  SRR4302535.4220545/94‑1 (MQ=60)
                                     ATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAAT                                                         <  SRR4302535.3617216/94‑1 (MQ=60)
                                     ATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAAT                                                         >  SRR4302535.3567460/1‑94 (MQ=60)
                                      TGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATA                                                        >  SRR4302535.2640709/1‑94 (MQ=60)
                                      TGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATA                                                        <  SRR4302535.3092618/94‑1 (MQ=60)
                                          TGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTC                                                    <  SRR4302535.3441352/94‑1 (MQ=60)
                                          TGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTC                                                    <  SRR4302535.1813258/94‑1 (MQ=60)
                                           GGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCT                                                   <  SRR4302535.934703/94‑1 (MQ=60)
                                           GGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCT                                                   <  SRR4302535.1659864/94‑1 (MQ=60)
                                              GAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTG                                                <  SRR4302535.3663590/94‑1 (MQ=60)
                                              GAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTG                                                <  SRR4302535.1672646/94‑1 (MQ=60)
                                               AAACTTGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGA                                               <  SRR4302535.3012077/94‑1 (MQ=60)
                                               AAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATCGCGAAAATATCTCTTTGA                                               <  SRR4302535.600527/94‑1 (MQ=60)
                                                 ACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTATGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATA                                             <  SRR4302535.610682/94‑1 (MQ=60)
                                                 ACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATA                                             <  SRR4302535.4204464/94‑1 (MQ=60)
                                                  CTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATAT                                            <  SRR4302535.724023/94‑1 (MQ=60)
                                                  CTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATAT                                            >  SRR4302535.3469365/1‑94 (MQ=60)
                                                  CTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATAT                                            <  SRR4302535.293936/94‑1 (MQ=60)
                                                    GGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCTTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCG                                          <  SRR4302535.3512895/94‑1 (MQ=60)
                                                    GGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCG                                          <  SRR4302535.4234913/94‑1 (MQ=60)
                                                       GAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGG                                       <  SRR4302535.3455662/94‑1 (MQ=60)
                                                         GTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAA                                     <  SRR4302535.2088150/94‑1 (MQ=60)
                                                          TAAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAA                                    >  SRR4302535.1866349/1‑94 (MQ=60)
                                                           AAACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAAT                                   >  SRR4302535.1162273/1‑94 (MQ=60)
                                                             ACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAG                                 <  SRR4302535.582771/94‑1 (MQ=60)
                                                             ACTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAG                                 <  SRR4302535.2678168/94‑1 (MQ=60)
                                                              CTGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGC                                <  SRR4302535.4390843/94‑1 (MQ=60)
                                                               TGACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCT                               <  SRR4302535.3107011/94‑1 (MQ=60)
                                                                GACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTT                              <  SRR4302535.365131/94‑1 (MQ=60)
                                                                 ACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTT                             >  SRR4302535.278114/1‑94 (MQ=60)
                                                                 ACCGTCAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTT                             <  SRR4302535.4456747/94‑1 (MQ=60)
                                                                      CAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCT                        >  SRR4302535.168821/1‑94 (MQ=60)
                                                                      CAACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCT                        <  SRR4302535.4302405/94‑1 (MQ=60)
                                                                       AACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTT                       <  SRR4302535.4656890/94‑1 (MQ=60)
                                                                       AACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTT                       <  SRR4302535.1526366/94‑1 (MQ=60)
                                                                       AACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTT                       >  SRR4302535.3450420/1‑94 (MQ=60)
                                                                       AACGCCCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGCTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTT                       >  SRR4302535.2499019/1‑94 (MQ=60)
                                                                            CCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCG                  <  SRR4302535.2691278/94‑1 (MQ=60)
                                                                            CCCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCG                  >  SRR4302535.2763828/1‑94 (MQ=60)
                                                                             CCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGG                 >  SRR4302535.1434069/1‑94 (MQ=60)
                                                                             CCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGG                 <  SRR4302535.3344329/94‑1 (MQ=60)
                                                                             CCCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGG                 >  SRR4302535.1710632/1‑94 (MQ=60)
                                                                              CCTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGT                >  SRR4302535.1640567/1‑94 (MQ=60)
                                                                               CTTGGCGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTC               >  SRR4302535.2273706/1‑94 (MQ=60)
                                                                                    CGCTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAG          >  SRR4302535.3864123/1‑94 (MQ=60)
                                                                                      CTACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGA        >  SRR4302535.2027987/1‑94 (MQ=60)
                                                                                        ACGGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGT      <  SRR4302535.768610/94‑1 (MQ=60)
                                                                                          GGACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGA    <  SRR4302535.1287482/94‑1 (MQ=60)
                                                                                            ACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAAC  <  SRR4302535.70359/94‑1 (MQ=60)
                                                                                            ACCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAAC  <  SRR4302535.3673230/94‑1 (MQ=60)
                                                                                            |                                                                                             
AAGCTGAATTAAAGCTGGAAAGTCGCGGAACCAACCAATGAATGGCGAAACTGGGGAGTAAACTGACCGTCAACGCCCCCTTGGCGCTACGGGCCTGGAGTTTACGCGTCGCTTCGGTTAATTGCGAAAATATCTCTTTGATATCGAGGAAATAGCTTTGCCCTTCCTCGGTCAGCAGGAGTGAAC  >  NC_000913/2940232‑2940417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑