Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,864	T→C	M169T (ATG→ACG)	b2810 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,864	0	T	C	100.0%	490.9 / NA	155	M169T (ATG→ACG)	b2810	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (69/86); total (69/86)

Only 100 of 162 total aligned reads displayed.

ATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTC  >  NC_000913/2941775‑2941953

aTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTc                                                                                        <  1:2645687/93‑1 (MQ=255)

 ttGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCg                                                                                       >  1:589356/1‑93 (MQ=255)

    ccGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAAc                                                                                    <  2:137408/93‑1 (MQ=255)

     cGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACg                                                                                   >  1:3961582/1‑93 (MQ=255)

      gCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGt                                                                                  >  2:4219165/1‑93 (MQ=255)

       cTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGtt                                                                                 >  2:3696591/1‑93 (MQ=255)

       cTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGtt                                                                                 <  1:2295535/93‑1 (MQ=255)

        ttAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTa                                                                                >  1:3788501/1‑93 (MQ=255)

         tAATGCGCAGCGACGGGCGTATGTCGATTTGATGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTAc                                                                               <  2:3404488/93‑1 (MQ=255)

          aaTGCGCAGCGACTGCCGGATGTCGATTTGTTGTCAGAACTGATTACTCCCCGTAGTCGGATTCTGTCGTTGGGCCAGGCGTCGAACGTTACt                                                                              >  2:863058/1‑93 (MQ=255)

          aaTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACt                                                                              <  1:2212956/93‑1 (MQ=255)

          aaTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTATGGCGTTGGGTCAGACGTCGAACGTTACt                                                                              >  2:3445599/1‑93 (MQ=255)

          aaTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGGAGTCGGATTCTGGCGTTGCGTTAGACGTCGAACGTTaat                                                                              >  1:615337/1‑91 (MQ=255)

           aTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTCCTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTg                                                                             <  2:3303485/93‑1 (MQ=255)

           aTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTg                                                                             <  2:4338821/93‑1 (MQ=255)

            tGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCTTTGGGTCAGACGTCGAACGTTACTgg                                                                            <  1:1942506/93‑1 (MQ=255)

              cgcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCg                                                                          <  2:1283065/93‑1 (MQ=255)

               gcAGCGACTGCTGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgg                                                                         <  2:2023/93‑1 (MQ=255)

               gcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgg                                                                         <  1:2204938/93‑1 (MQ=255)

                cAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGt                                                                        >  1:1054547/1‑93 (MQ=255)

                cAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGt                                                                        <  2:3588616/93‑1 (MQ=255)

                 aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                       <  2:3131975/93‑1 (MQ=255)

                    gACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTggc                                                                    >  1:860717/1‑91 (MQ=255)

                     aCTGCTGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGccc                                                                   <  2:2407599/93‑1 (MQ=255)

                        gCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGa                                                                <  2:3292181/93‑1 (MQ=255)

                          cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATc                                                              <  1:4022762/93‑1 (MQ=255)

                           ggATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCt                                                             <  1:4565633/93‑1 (MQ=255)

                            gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTg                                                            >  2:2347700/1‑93 (MQ=255)

                            gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTg                                                            <  2:2402252/93‑1 (MQ=255)

                            gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTg                                                            <  1:3541159/93‑1 (MQ=255)

                            gATGTCGATTTGTTGACAGAACTGATTACGCCCCGTAGTCGGATTCTGGGGTTGGGTGAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTg                                                            <  2:3974326/93‑1 (MQ=255)

                             aTGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGTGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTgg                                                           <  1:4344735/93‑1 (MQ=255)

                             aTGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTgg                                                           >  2:323161/1‑93 (MQ=255)

                             aTGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTgg                                                           >  1:3179045/1‑93 (MQ=255)

                             aTGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTgg                                                           <  2:3769199/93‑1 (MQ=255)

                               gTCGATTTGCTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGgcg                                                         >  1:4682054/1‑93 (MQ=255)

                                tCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGCTCTGgcgc                                                        >  2:1512764/1‑93 (MQ=255)

                                   aTTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgag                                                     <  1:2928271/93‑1 (MQ=255)

                                   aTTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgag                                                     >  1:2841875/1‑93 (MQ=255)

                                   aTTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgag                                                     <  2:1840331/93‑1 (MQ=255)

                                    tttGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagc                                                    <  2:1896653/93‑1 (MQ=255)

                                     ttgttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagcg                                                   <  1:3109961/93‑1 (MQ=255)

                                     ttgttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagcg                                                   <  2:2445610/93‑1 (MQ=255)

                                       gttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTAGCGGATGCCCGGATCCGGCGCGAGCGAt                                                 >  2:2818165/1‑93 (MQ=255)

                                        ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                                >  2:4475626/1‑93 (MQ=255)

                                        ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                                >  2:3185358/1‑93 (MQ=255)

                                        ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                                <  2:4282796/93‑1 (MQ=255)

                                        ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                                <  1:3335121/93‑1 (MQ=255)

                                          gCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAc                                              >  1:3661275/1‑93 (MQ=255)

                                           ccAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAcc                                             <  2:3962579/93‑1 (MQ=255)

                                           ccAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAcc                                             <  1:1666632/93‑1 (MQ=255)

                                            cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                            <  1:3664878/93‑1 (MQ=255)

                                             aGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCtt                                           <  2:1197406/93‑1 (MQ=255)

                                                aCTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTgt                                        >  1:2980374/1‑92 (MQ=255)

                                                aCTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGc                                        >  1:3202665/1‑93 (MQ=255)

                                                   gATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCa                                     <  1:878815/93‑1 (MQ=255)

                                                   gATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCa                                     <  2:3319744/93‑1 (MQ=255)

                                                   gATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCa                                     >  1:1386762/1‑93 (MQ=255)

                                                    aTTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGGGCGGATCTGGGGCGGGCGATTACCTTTGCTcgt                                    >  2:4290710/1‑91 (MQ=255)

                                                    aTTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAt                                    >  1:2770641/1‑93 (MQ=255)

                                                    aTTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAt                                    >  1:1996946/1‑93 (MQ=255)

                                                    aTTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAt                                    <  1:1725697/93‑1 (MQ=255)

                                                      tACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTc                                  <  2:2397815/93‑1 (MQ=255)

                                                       tcTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCa                                 <  1:251076/92‑1 (MQ=255)

                                                        cTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAg                                <  2:7081/93‑1 (MQ=255)

                                                        cTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAg                                <  2:1590672/93‑1 (MQ=255)

                                                         tCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCGGTTACCTTTGCTCATTCatc                               >  2:54558/1‑91 (MQ=255)

                                                         tCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCCTTCAGc                               >  1:3682544/1‑93 (MQ=255)

                                                         tCCCCGTAGTCGGATTCTGGCGTTAGGACAGACGTCGAACATTAATGGCAGATGCCCGGATATGGCGCGAGCGATTACCTTTGCTCATTCAGc                               <  1:4685696/93‑1 (MQ=255)

                                                         cccccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGc                               <  1:1127511/92‑1 (MQ=255)

                                                           cccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCg                             >  2:4084526/1‑93 (MQ=255)

                                                            ccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgg                            <  2:1664637/93‑1 (MQ=255)

                                                            ccGCAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgg                            <  1:2384620/93‑1 (MQ=255)

                                                              gTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgggg                          >  2:3779232/1‑92 (MQ=255)

                                                              gTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGa                          <  2:1479481/93‑1 (MQ=255)

                                                              gTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGa                          <  2:3973169/93‑1 (MQ=255)

                                                              gTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGa                          <  1:1701802/93‑1 (MQ=255)

                                                                aGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGGCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGGtg                        >  1:1828735/1‑93 (MQ=255)

                                                                 gTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtgg                       <  1:815213/93‑1 (MQ=255)

                                                                   gggATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGCATCGGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtg                     <  2:3336617/92‑1 (MQ=255)

                                                                   cGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtg                     <  2:3614152/93‑1 (MQ=255)

                                                                   cGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtg                     <  2:2902390/93‑1 (MQ=255)

                                                                   cGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGATGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGCtggtg                     >  1:2360787/1‑93 (MQ=255)

                                                                    ggATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtgg                    >  1:1852725/1‑93 (MQ=255)

                                                                     gATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATggtggt                   >  2:2113845/1‑93 (MQ=255)

                                                                     gATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATggtggt                   >  1:2136226/1‑93 (MQ=255)

                                                                      aTTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGgtggtg                  <  1:389261/93‑1 (MQ=255)

                                                                       ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtga                 >  1:3768072/1‑93 (MQ=255)

                                                                       ttCTGGCGTTGGGTCAGACGTCGAACGGTACTGGCGGGTGCCCGGTACTTGCGCGGGCGGGTACCCTTGTTCATTCAGGCCGGGTGggggtgg                 >  2:29790/1‑92 (MQ=255)

                                                                        tCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgat                <  1:1453907/93‑1 (MQ=255)

                                                                         cTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCGGCCGGGATGGTGGtgatg               <  1:343136/93‑1 (MQ=255)

                                                                               ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat         <  2:2165772/93‑1 (MQ=255)

                                                                               ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat         <  2:1681845/93‑1 (MQ=255)

                                                                               ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCAGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGcgatggttgag         >  2:1536794/1‑92 (MQ=255)

                                                                                tgcgTCAGACGTCTAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGggatggttgatg        <  2:408280/90‑1 (MQ=255)

                                                                                tGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGGGGtgatggttgatg        >  1:3050130/1‑93 (MQ=255)

                                                                                  ggTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgatggt      <  1:1922617/93‑1 (MQ=255)

                                                                                   gTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTg     >  2:3164263/1‑93 (MQ=255)

                                                                                      aGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTc  >  2:4542463/1‑93 (MQ=255)

                                                                                      aGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTc  >  1:4387074/1‑93 (MQ=255)

ATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTC  >  NC_000913/2941775‑2941953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGG  >  NC_000913/2941771‑2941957

TGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGAC                                                                                               >  SRR4302535.405453/1‑94 (MQ=60)

TGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGAC                                                                                               >  SRR4302535.3087532/1‑94 (MQ=60)

TGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGAC                                                                                               <  SRR4302535.2408050/94‑1 (MQ=60)

TGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGAC                                                                                               <  SRR4302535.1241669/94‑1 (MQ=60)

 TAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACG                                                                                              <  SRR4302535.961710/94‑1 (MQ=60)

 GAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACG                                                                                              >  SRR4302535.4331695/1‑94 (MQ=60)

 GAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACG                                                                                              <  SRR4302535.2323649/94‑1 (MQ=60)

   aattcgccCTTAATGCGCAGCGACTGCCGGATGTCTATTTGTTGCCAGAACTGATTGCTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTC                                                                                            <  SRR4302535.1183864/86‑1 (MQ=60)

    ATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCG                                                                                           >  SRR4302535.593487/1‑94 (MQ=60)

    ATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCG                                                                                           <  SRR4302535.2664635/94‑1 (MQ=60)

       GCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGGAGTCGGATTCTGGCGTTGGGTCAGTCGTCGAAC                                                                                        >  SRR4302535.4470103/1‑94 (MQ=60)

        CCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACG                                                                                       <  SRR4302535.723474/94‑1 (MQ=60)

        CCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACG                                                                                       >  SRR4302535.3988636/1‑94 (MQ=60)

          GCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGAGTCTGGCGTTGGGTCAGACGTCGAACGTT                                                                                     >  SRR4302535.841643/1‑94 (MQ=60)

           CTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTA                                                                                    >  SRR4302535.3814631/1‑94 (MQ=60)

           CTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTA                                                                                    <  SRR4302535.2311957/94‑1 (MQ=60)

             TAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACT                                                                                  >  SRR4302535.91841/1‑94 (MQ=60)

             TAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGGAGTCGGATTCTGGCGTTGCGTTAGACGTCGAACGTTAAT                                                                                  >  SRR4302535.619617/1‑94 (MQ=60)

              AATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTG                                                                                 <  SRR4302535.2228715/94‑1 (MQ=60)

                TGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCTTTGGGTCAGACGTCGAACGTTACTGGC                                                                               <  SRR4302535.1956497/94‑1 (MQ=60)

                   GCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGT                                                                            <  SRR4302535.2220643/94‑1 (MQ=60)

                   GCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGT                                                                            >  SRR4302535.1062047/1‑94 (MQ=60)

                     AGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTG                                                                          >  SRR4302535.3195992/1‑94 (MQ=60)

                     AGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTG                                                                          <  SRR4302535.1744313/94‑1 (MQ=60)

                       CGACTGCCGGATGTTGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCC                                                                        <  SRR4302535.4496244/94‑1 (MQ=60)

                       CGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGGC                                                                        >  SRR4302535.866798/1‑94 (MQ=60)

                              CGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCT                                                                 <  SRR4302535.2278621/94‑1 (MQ=60)

                              CGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCT                                                                 <  SRR4302535.3105524/94‑1 (MQ=60)

                              CGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCT                                                                 <  SRR4302535.4050284/94‑1 (MQ=60)

                               GGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTG                                                                <  SRR4302535.4596781/94‑1 (MQ=60)

                                GATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGG                                                               <  SRR4302535.3565765/94‑1 (MQ=60)

                                GATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGG                                                               >  SRR4302535.3201027/1‑94 (MQ=60)

                                GATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGG                                                               <  SRR4302535.2068965/94‑1 (MQ=60)

                                 ATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGTGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGC                                                              <  SRR4302535.4374460/94‑1 (MQ=60)

                                  TGTCGATTTGCTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCG                                                             >  SRR4302535.4713697/1‑94 (MQ=60)

                                      GATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAG                                                         >  SRR4302535.2862032/1‑94 (MQ=60)

                                       ATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGC                                                        <  SRR4302535.2948907/94‑1 (MQ=60)

                                         TTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGA                                                      <  SRR4302535.3131612/94‑1 (MQ=60)

                                          TGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGCCCGGATCTGGCGCGAGCGAT                                                     >  SRR4302535.2449784/1‑94 (MQ=60)

                                            TTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTA                                                   <  SRR4302535.3358347/94‑1 (MQ=60)

                                             TGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGGGGGCGGTTAC                                                  >  SRR4302535.47905/1‑94 (MQ=60)

                                             TGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTAC                                                  >  SRR4302535.3686620/1‑94 (MQ=60)

                                               CCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTGCCT                                                <  SRR4302535.3707327/94‑1 (MQ=60)

                                               CCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCT                                                <  SRR4302535.1678691/94‑1 (MQ=60)

                                               CCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCT                                                >  SRR4302535.2998081/1‑94 (MQ=60)

                                                CAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTT                                               <  SRR4302535.3690257/94‑1 (MQ=60)

                                                 AGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTT                                              <  SRR4302535.3677058/94‑1 (MQ=60)

                                                 AGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTT                                              <  SRR4302535.3286668/94‑1 (MQ=60)

                                                  GAAGTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTG                                             <  SRR4302535.3828982/94‑1 (MQ=60)

                                                   AACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGT                                            >  SRR4302535.3001282/1‑94 (MQ=60)

                                                   AACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGC                                            >  SRR4302535.3224838/1‑94 (MQ=60)

                                                   AACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGC                                            >  SRR4302535.283933/1‑94 (MQ=60)

                                                     CTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCcgagcttgcgcgggcgatattctttgctc                                          >  SRR4302535.4026548/1‑65 (MQ=60)

                                                     CTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCGGTTACCTTTGCTC                                          >  SRR4302535.1645779/1‑94 (MQ=60)

                                                      TGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCA                                         <  SRR4302535.4647098/94‑1 (MQ=60)

                                                      TGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCA                                         >  SRR4302535.1396659/1‑94 (MQ=60)

                                                       GATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAT                                        >  SRR4302535.239635/1‑94 (MQ=60)

                                                       GATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAT                                        >  SRR4302535.2011322/1‑94 (MQ=60)

                                                       GATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAT                                        >  SRR4302535.2790372/1‑94 (MQ=60)

                                                       GATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAT                                        <  SRR4302535.885036/94‑1 (MQ=60)

                                                        ATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGACCGTTACTGGCGGTTGGCCGGATCTGGCGCGGGCGATTACCTTTGCTCATT                                       >  SRR4302535.4143819/1‑94 (MQ=60)

                                                        ATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATT                                       <  SRR4302535.1738176/94‑1 (MQ=60)

                                                          TACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCA                                     >  SRR4302535.1952645/1‑94 (MQ=60)

                                                           TCTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAG                                    <  SRR4302535.252804/94‑1 (MQ=60)

                                                           ACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAG                                    >  SRR4302535.3792230/1‑94 (MQ=60)

                                                            CTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCCTTCAGC                                   >  SRR4302535.3708028/1‑94 (MQ=60)

                                                             TCCCCGTAGTCGGATTCTGGCGTTAGGACAGACGTCGAACATTAATGGCAGATGCCCGGATATGGCGCGAGCGATTACCTTTGCTCATTCAGCC                                  <  SRR4302535.4717427/94‑1 (MQ=60)

                                                             CCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCC                                  <  SRR4302535.1135562/94‑1 (MQ=60)

                                                                CCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGG                               >  SRR4302535.2159301/1‑94 (MQ=60)

                                                                CCGCAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGG                               <  SRR4302535.2401746/94‑1 (MQ=60)

                                                                 CGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGA                              >  SRR4302535.1701266/1‑94 (MQ=60)

                                                                  GTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGCGCGATTACCTTTGCTCATTCAGCCGGGAT                             >  SRR4302535.356037/1‑94 (MQ=60)

                                                                  GTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAT                             <  SRR4302535.1714105/94‑1 (MQ=60)

                                                                   TAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGGCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGGTG                            >  SRR4302535.1841937/1‑94 (MQ=60)

                                                                    TGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGG                           <  SRR4302535.1932760/94‑1 (MQ=60)

                                                                     GTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGT                          <  SRR4302535.820929/94‑1 (MQ=60)

                                                                     GTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCCGATCTGGCGCGAGCGATTACCCTTGTTCCTTCAGCCGGGATGGG                          >  SRR4302535.1354578/1‑94 (MQ=60)

                                                                      TCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGATGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGCTGGTG                         >  SRR4302535.2377732/1‑94 (MQ=60)

                                                                       CGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGG                        >  SRR4302535.1866071/1‑94 (MQ=60)

                                                                        GGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGT                       >  SRR4302535.2151615/1‑94 (MQ=60)

                                                                        GGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGT                       <  SRR4302535.2727833/94‑1 (MQ=60)

                                                                          ATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGA                     >  SRR4302535.3794086/1‑94 (MQ=60)

                                                                          ATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGA                     <  SRR4302535.391968/94‑1 (MQ=60)

                                                                            TCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATG                   <  SRR4302535.1464311/94‑1 (MQ=60)

                                                                             CTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCGGCCGGGATGGTGGTGATGG                  <  SRR4302535.345508/94‑1 (MQ=60)

                                                                                   TTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGGGGTGATGGTTGATG            >  SRR4302535.3071494/1‑94 (MQ=60)

                                                                                      GGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTG         <  SRR4302535.1936477/94‑1 (MQ=60)

                                                                                         CAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTC      >  SRR4302535.4417084/1‑94 (MQ=60)

                                                                                           GACGTCGAACGTTACTGGCGGTTGCCCGGATCTTGCGCGAGCGGTTCAATTTGCTCATTCAGCCGGGGTGGCGGTGCTGGTTGATGGTGCTCAG    >  SRR4302535.2805432/1‑94 (MQ=57)

                                                                                             CGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTTCTCATTCAGCCGGGATGGTGGGGATGGTTGATGGTGCTCAGGG  >  SRR4302535.1213329/1‑94 (MQ=60)

                                                                                             CGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGG  <  SRR4302535.3411290/94‑1 (MQ=60)

TGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGG  >  NC_000913/2941771‑2941957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 5 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`