Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,019,825 G→A P163P (CCG→CCA)  b2881 → putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,019,8250GA99.0% 722.6 / ‑4.3 205P163P (CCG→CCA) b2881putative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (74/129);  total (74/131)
Fisher's exact test for biased strand distribution p-value = 5.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

Only 100 of 214 total aligned reads displayed.

CCGCGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGA  >  NC_000913/3019735‑3019914
                                                                                          |                                                                                         
ccGCGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAAc                                                                                         <  1:2672722/93‑1 (MQ=255)
  gcgATGCTGGCTAGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATTATCCGCAGGCCACCATTCATATCGCTCCAACtt                                                                                       >  2:3133075/1‑93 (MQ=255)
    gATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTc                                                                                     <  1:3811624/93‑1 (MQ=255)
    caTGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTc                                                                                     <  2:2134273/92‑1 (MQ=255)
     aTGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTcc                                                                                    >  1:299570/1‑93 (MQ=255)
      tGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGGCACCATTGATATCGCTCCAACTTTCCg                                                                                   >  2:250184/1‑93 (MQ=255)
      tGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCg                                                                                   >  2:940301/1‑93 (MQ=255)
       gctggctgGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGt                                                                                  <  2:1271418/93‑1 (MQ=255)
       gctggctgGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGt                                                                                  <  1:4271858/93‑1 (MQ=255)
        ctggctggCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCAACCATTGATATCGCTCCAACTTTCCGTg                                                                                 >  2:4305286/1‑93 (MQ=255)
         tggctggcAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTga                                                                                <  1:3561974/93‑1 (MQ=255)
         tggctggcAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTga                                                                                >  1:3922866/1‑93 (MQ=255)
          ggctggcAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTgac                                                                               <  1:3586288/93‑1 (MQ=255)
             tggcAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGGCACCATTGATATCGCTCCAACTTTCCGTgacgac                                                                            >  2:1750909/1‑93 (MQ=255)
             tggcAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTgacgac                                                                            >  1:20937/1‑93 (MQ=255)
             tggcAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTgacgac                                                                            >  1:3863280/1‑93 (MQ=255)
                cagcaATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTa                                                                         <  1:3674208/93‑1 (MQ=255)
                  gcaATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGAACTAGa                                                                       >  2:3212055/1‑93 (MQ=255)
                   caATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGaa                                                                      <  1:2907667/93‑1 (MQ=255)
                    aaTACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAg                                                                     <  1:1834036/93‑1 (MQ=255)
                    aaTACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAg                                                                     <  1:1529337/93‑1 (MQ=255)
                    aaTACTATCAGGTCATTGAACTGGCGGTTCCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAg                                                                     <  2:645994/93‑1 (MQ=255)
                     aTACTATCAGGTCATTGAACTGGCGGTTGCACGGAAAAATAATACGCAGGCCACCATTGATATCGCTCCAACTTTACGTGACGACCTAGAAgc                                                                    >  2:783571/1‑92 (MQ=255)
                      tACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTc                                                                   >  1:577135/1‑93 (MQ=255)
                      tACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTc                                                                   <  1:4154283/93‑1 (MQ=255)
                      tACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTc                                                                   <  1:3714397/93‑1 (MQ=255)
                      tACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTc                                                                   >  2:1922816/1‑93 (MQ=255)
                        cTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCAt                                                                 >  1:3057080/1‑93 (MQ=255)
                         tATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCAtt                                                                <  2:3545551/93‑1 (MQ=255)
                         tATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCAtt                                                                >  2:2289977/1‑93 (MQ=255)
                         tATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCAtt                                                                <  2:398037/93‑1 (MQ=255)
                         tATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCAtt                                                                <  1:2723872/93‑1 (MQ=255)
                          aTCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTg                                                               >  2:136415/1‑93 (MQ=255)
                              ggTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTaa                                                           <  2:4368537/93‑1 (MQ=255)
                              ggTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTaa                                                           >  2:1329869/1‑93 (MQ=255)
                              ggTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTaa                                                           <  1:2827131/93‑1 (MQ=255)
                                 cATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCa                                                        <  1:3300667/93‑1 (MQ=255)
                                  aTTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAt                                                       <  2:2759045/93‑1 (MQ=255)
                                  aTTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAt                                                       <  2:1687547/93‑1 (MQ=255)
                                  aTTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAt                                                       >  1:1034439/1‑93 (MQ=255)
                                  aTTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAt                                                       >  2:4543784/1‑93 (MQ=255)
                                  aTTGAACTGGCAGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAt                                                       <  1:4681314/93‑1 (MQ=255)
                                   ttGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAtt                                                      >  1:1141770/1‑93 (MQ=255)
                                   ttGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAtt                                                      <  1:2558836/93‑1 (MQ=255)
                                   ttGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAtt                                                      >  1:806457/1‑93 (MQ=255)
                                     gAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTAt                                                    <  2:2723995/93‑1 (MQ=255)
                                     gAACTGGCGCTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTAt                                                    <  2:2641754/93‑1 (MQ=255)
                                       aCTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATcc                                                  >  2:577624/1‑93 (MQ=255)
                                        cTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCt                                                 <  2:4093643/93‑1 (MQ=255)
                                              gTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACt                                           >  2:3369429/1‑93 (MQ=255)
                                                tGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGa                                         <  2:202461/93‑1 (MQ=255)
                                                 gCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGAt                                        <  1:639663/93‑1 (MQ=255)
                                                 gCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGAt                                        <  1:1974956/93‑1 (MQ=255)
                                                 gCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGAt                                        >  2:890443/1‑93 (MQ=255)
                                                   aCGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGc                                      <  2:3244488/93‑1 (MQ=255)
                                                      tAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCcgc                                   >  1:3397591/1‑93 (MQ=255)
                                                        aaaaTAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGa                                 <  2:4444349/93‑1 (MQ=255)
                                                         aaaTAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGaa                                <  2:1984542/93‑1 (MQ=255)
                                                           ataatCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGaaaa                              >  2:506857/1‑93 (MQ=255)
                                                             aatCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATg                            <  2:2889140/93‑1 (MQ=255)
                                                             aatCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATg                            <  2:1496187/93‑1 (MQ=255)
                                                             aatCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATg                            <  2:1635716/93‑1 (MQ=255)
                                                              atCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATgg                           <  2:3813175/93‑1 (MQ=255)
                                                              atCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATgg                           <  1:1920213/93‑1 (MQ=255)
                                                              atCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATgg                           <  2:2337993/93‑1 (MQ=255)
                                                              atCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATgg                           <  1:1167110/93‑1 (MQ=255)
                                                              atCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATgg                           >  1:3772556/1‑93 (MQ=255)
                                                              atCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATgg                           >  1:484077/1‑93 (MQ=255)
                                                               tCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGt                          <  1:443492/93‑1 (MQ=255)
                                                               tCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGt                          >  2:1742301/1‑93 (MQ=255)
                                                                 cGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGc                        >  1:4204422/1‑93 (MQ=255)
                                                                 cGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGc                        <  1:555044/93‑1 (MQ=255)
                                                                 cGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGc                        <  1:93344/93‑1 (MQ=255)
                                                                  gCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCa                       <  2:4231831/93‑1 (MQ=255)
                                                                  gCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCa                       >  2:4607884/1‑93 (MQ=255)
                                                                  gCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCa                       >  2:1614803/1‑93 (MQ=255)
                                                                  gCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCa                       >  1:2763120/1‑93 (MQ=255)
                                                                    aGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAgg                     <  2:1859184/93‑1 (MQ=255)
                                                                    aGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAgg                     <  1:1668172/93‑1 (MQ=255)
                                                                     ggCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGc                    >  2:2974350/1‑93 (MQ=255)
                                                                     ggCCACCATCGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGc                    <  2:257045/93‑1 (MQ=255)
                                                                         cacaTTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGaaa                <  2:3506631/91‑1 (MQ=255)
                                                                         accaTTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGaaa                >  1:2413164/1‑93 (MQ=255)
                                                                         accaTTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGaaa                <  1:3907216/93‑1 (MQ=255)
                                                                           caTTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAAcc              <  1:615177/93‑1 (MQ=255)
                                                                             ttGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCt            <  2:2409720/93‑1 (MQ=255)
                                                                               gATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGc          <  2:3714716/93‑1 (MQ=255)
                                                                                atatCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCt         >  1:3208367/1‑93 (MQ=255)
                                                                                 tatCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTa        >  1:4460481/1‑93 (MQ=255)
                                                                                 tatCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTa        <  2:3669907/93‑1 (MQ=255)
                                                                                  atCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTAt       <  2:742018/93‑1 (MQ=255)
                                                                                    cGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGt     <  1:4494989/93‑1 (MQ=255)
                                                                                    cGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGt     <  1:4394749/93‑1 (MQ=255)
                                                                                    cGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGt     <  1:2974740/93‑1 (MQ=255)
                                                                                     gCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGtt    >  1:2334607/1‑93 (MQ=255)
                                                                                      cTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTg   >  2:1763598/1‑93 (MQ=255)
                                                                                      cTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTg   <  1:4367535/93‑1 (MQ=255)
                                                                                      cTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTg   <  1:133063/93‑1 (MQ=255)
                                                                                       tCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGa  <  2:1448902/93‑1 (MQ=255)
                                                                                       tCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGa  >  1:2199654/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCGCGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGA  >  NC_000913/3019735‑3019914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCGCGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAG  >  NC_000913/3019734‑3019916
                                                                                           |                                                                                           
GCCGCGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAAC                                                                                           >  SRR4302535.321122/1‑94 (MQ=60)
 CCGCGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACT                                                                                          <  SRR4302535.2691843/94‑1 (MQ=60)
 CCGCGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACT                                                                                          <  SRR4302535.4414046/94‑1 (MQ=60)
    CGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTC                                                                                       >  SRR4302535.2732257/1‑94 (MQ=60)
    CGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTC                                                                                       >  SRR4302535.4432462/1‑94 (MQ=60)
     GATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCC                                                                                      >  SRR4302535.301658/1‑94 (MQ=60)
     GATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCC                                                                                      <  SRR4302535.3837922/94‑1 (MQ=60)
        GCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTG                                                                                   <  SRR4302535.4717001/94‑1 (MQ=60)
        GCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTG                                                                                   <  SRR4302535.4301103/94‑1 (MQ=60)
         CTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGA                                                                                  >  SRR4302535.3949677/1‑94 (MQ=60)
          TGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGAC                                                                                 <  SRR4302535.3586674/94‑1 (MQ=60)
          TGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGAC                                                                                 >  SRR4302535.3939262/1‑94 (MQ=60)
           GGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACG                                                                                <  SRR4302535.3611164/94‑1 (MQ=60)
             CTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGAC                                                                              >  SRR4302535.3889858/1‑94 (MQ=60)
             CTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGAC                                                                              >  SRR4302535.21092/1‑94 (MQ=60)
               GGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCT                                                                            <  SRR4302535.902528/94‑1 (MQ=60)
                GCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTA                                                                           <  SRR4302535.510666/94‑1 (MQ=60)
                 CAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAG                                                                          <  SRR4302535.3699649/94‑1 (MQ=60)
                    gaatacgagcaggctagTGAACTGGCGGTTGCACGGAAAAATAAGCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAG                                                                       <  SRR4302535.2144643/77‑1 (MQ=60)
                    CAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAG                                                                       >  SRR4302535.3417275/1‑94 (MQ=60)
                    CAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAG                                                                       <  SRR4302535.4203627/94‑1 (MQ=60)
                    CAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAG                                                                       <  SRR4302535.2928218/94‑1 (MQ=60)
                     AATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGT                                                                      <  SRR4302535.564550/94‑1 (MQ=60)
                     AATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGT                                                                      <  SRR4302535.1540354/94‑1 (MQ=60)
                     AATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGT                                                                      <  SRR4302535.1847269/94‑1 (MQ=60)
                      ATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTC                                                                     >  SRR4302535.581190/1‑94 (MQ=60)
                       TACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCA                                                                    <  SRR4302535.3740131/94‑1 (MQ=60)
                       TACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCA                                                                    <  SRR4302535.4182724/94‑1 (MQ=60)
                       TACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCA                                                                    >  SRR4302535.2950496/1‑94 (MQ=60)
                        ACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCAT                                                                   >  SRR4302535.3078472/1‑94 (MQ=60)
                         CTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATT                                                                  <  SRR4302535.3721101/94‑1 (MQ=60)
                         CTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATT                                                                  <  SRR4302535.3906121/94‑1 (MQ=60)
                          TATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTG                                                                 <  SRR4302535.3200892/94‑1 (MQ=60)
                          TATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTG                                                                 <  SRR4302535.2743326/94‑1 (MQ=60)
                           ATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGG                                                                <  SRR4302535.470321/94‑1 (MQ=60)
                             CAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTA                                                              >  SRR4302535.200177/1‑94 (MQ=60)
                               GGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAG                                                            <  SRR4302535.2847196/94‑1 (MQ=60)
                                  CATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAT                                                         >  SRR4302535.1041786/1‑94 (MQ=60)
                                  CATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCAT                                                         <  SRR4302535.3323619/94‑1 (MQ=60)
                                   ATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATT                                                        >  SRR4302535.1149908/1‑94 (MQ=60)
                                   ATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATT                                                        >  SRR4302535.812115/1‑94 (MQ=60)
                                   ATTGAACTGGCAGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATT                                                        <  SRR4302535.4712943/94‑1 (MQ=60)
                                    TTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTA                                                       <  SRR4302535.2577256/94‑1 (MQ=60)
                                        ACTGGCGGTTGCACGTAAAAATAATTCGCAAGGCCCCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCGTTATCCT                                                   >  SRR4302535.2470798/1‑94 (MQ=60)
                                        ACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCT                                                   <  SRR4302535.19667/94‑1 (MQ=60)
                                        ACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCT                                                   <  SRR4302535.1335064/94‑1 (MQ=60)
                                        ACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCT                                                   <  SRR4302535.2320373/94‑1 (MQ=60)
                                        ACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCT                                                   <  SRR4302535.478429/94‑1 (MQ=60)
                                          TGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAA                                                 >  SRR4302535.2415912/1‑94 (MQ=60)
                                               GTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTG                                            <  SRR4302535.1149965/94‑1 (MQ=60)
                                                  TCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATG                                         <  SRR4302535.2741516/94‑1 (MQ=60)
                                                  GCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATG                                         <  SRR4302535.644133/94‑1 (MQ=60)
                                                  GCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATG                                         <  SRR4302535.1989181/94‑1 (MQ=60)
                                                   CACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGC                                        <  SRR4302535.196581/94‑1 (MQ=60)
                                                    ACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCC                                       <  SRR4302535.4029329/94‑1 (MQ=60)
                                                      GTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGC                                     >  SRR4302535.2832596/1‑94 (MQ=60)
                                                      GTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGC                                     >  SRR4302535.1404200/1‑94 (MQ=60)
                                                      GTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGC                                     >  SRR4302535.3421233/1‑94 (MQ=60)
                                                       TAAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCG                                    <  SRR4302535.616193/94‑1 (MQ=60)
                                                        AAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGA                                   >  SRR4302535.3661155/1‑94 (MQ=60)
                                                        AAAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGA                                   >  SRR4302535.2678705/1‑94 (MQ=60)
                                                         AAAATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAA                                  >  SRR4302535.3040242/1‑94 (MQ=60)
                                                           AATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAA                                >  SRR4302535.1864060/1‑94 (MQ=60)
                                                            ATAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAAT                               <  SRR4302535.374915/94‑1 (MQ=60)
                                                             TAATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATG                              <  SRR4302535.3793069/94‑1 (MQ=60)
                                                              AATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGG                             >  SRR4302535.3798577/1‑94 (MQ=60)
                                                              AATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGG                             >  SRR4302535.487469/1‑94 (MQ=60)
                                                               ATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGT                            <  SRR4302535.1934052/94‑1 (MQ=60)
                                                               ATCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGT                            <  SRR4302535.1175443/94‑1 (MQ=60)
                                                                TCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTG                           <  SRR4302535.3586819/94‑1 (MQ=60)
                                                                TCCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTG                           <  SRR4302535.446596/94‑1 (MQ=60)
                                                                 CCGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGC                          >  SRR4302535.4233199/1‑94 (MQ=60)
                                                                  CGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCA                         <  SRR4302535.94006/94‑1 (MQ=60)
                                                                  CGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCA                         <  SRR4302535.558934/94‑1 (MQ=60)
                                                                  CGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCA                         <  SRR4302535.4467531/94‑1 (MQ=60)
                                                                  CGCAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCA                         >  SRR4302535.2782807/1‑94 (MQ=60)
                                                                    CAGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGG                       >  SRR4302535.2768938/1‑94 (MQ=60)
                                                                     AGGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGC                      <  SRR4302535.1680243/94‑1 (MQ=60)
                                                                      GGCCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCG                     <  SRR4302535.3291535/94‑1 (MQ=60)
                                                                        CCACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAA                   >  SRR4302535.170999/1‑94 (MQ=60)
                                                                         CACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAA                  >  SRR4302535.2430538/1‑94 (MQ=60)
                                                                          ACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAAC                 <  SRR4302535.3933985/94‑1 (MQ=60)
                                                                          ACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAAC                 <  SRR4302535.3817196/94‑1 (MQ=60)
                                                                          ACCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAAC                 <  SRR4302535.4525053/94‑1 (MQ=60)
                                                                           CCATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACC                >  SRR4302535.621905/1‑94 (MQ=60)
                                                                            CATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCC               <  SRR4302535.619457/94‑1 (MQ=60)
                                                                             ATTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCT              <  SRR4302535.3843162/94‑1 (MQ=60)
                                                                              TTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTG             >  SRR4302535.108863/1‑94 (MQ=60)
                                                                              TTGATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTG             <  SRR4302535.1650089/94‑1 (MQ=60)
                                                                                GATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCT           >  SRR4302535.3230589/1‑94 (MQ=60)
                                                                                GATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCT           <  SRR4302535.784515/94‑1 (MQ=60)
                                                                                 ATATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTA          >  SRR4302535.4491074/1‑94 (MQ=60)
                                                                                  TATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTAT         <  SRR4302535.4307538/94‑1 (MQ=60)
                                                                                   ATCGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATG        >  SRR4302535.4290104/1‑94 (MQ=60)
                                                                                     CGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTT      <  SRR4302535.4525842/94‑1 (MQ=60)
                                                                                     CGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTT      <  SRR4302535.2995618/94‑1 (MQ=60)
                                                                                     CGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTT      >  SRR4302535.2882669/1‑94 (MQ=60)
                                                                                     CGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTT      >  SRR4302535.2351315/1‑94 (MQ=60)
                                                                                     CGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTT      <  SRR4302535.4424824/94‑1 (MQ=60)
                                                                                     CGCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTT      >  SRR4302535.4240537/1‑94 (MQ=60)
                                                                                      GCTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTG     >  SRR4302535.2998601/1‑94 (MQ=60)
                                                                                       CTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGA    >  SRR4302535.2215330/1‑94 (MQ=60)
                                                                                       CTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGA    <  SRR4302535.133996/94‑1 (MQ=60)
                                                                                       CTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGA    <  SRR4302535.4397423/94‑1 (MQ=60)
                                                                                       CTCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGA    >  SRR4302535.3517884/1‑94 (MQ=60)
                                                                                        TCCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAA   >  SRR4302535.1581093/1‑94 (MQ=60)
                                                                                         CCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAG  <  SRR4302535.4450350/94‑1 (MQ=60)
                                                                                         CCAACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAG  <  SRR4302535.422751/94‑1 (MQ=60)
                                                                                           |                                                                                           
GCCGCGATGCTGGCTGGCAGCAATACTATCAGGTCATTGAACTGGCGGTTGCACGTAAAAATAATCCGCAGGCCACCATTGATATCGCTCCGACTTTCCGTGACGACCTAGAAGTCATTGGTAAGCATTATCCTAAAACTGATGCCGCGAAAATGGTGCAGGCGAAACCCTGCTATGTTGAAG  >  NC_000913/3019734‑3019916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑