Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,099,661 (A)5→6 intergenic (‑19/+164) yggN ← / ← b2959 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,099,6561.A100.0% 722.4 / NA 195intergenic (‑14/+169)yggN/b2959hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (103/92);  total (103/92)

Only 100 of 218 total aligned reads displayed.

TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC  >  NC_000913/3099564‑3099743
                                                                                             |                                                                                       
 gaggCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑a                                                                                        <  2:921257/90‑1 (MQ=255)
 gACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑a                                                                                        <  2:2062166/93‑1 (MQ=255)
 gACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑a                                                                                        <  1:4147993/93‑1 (MQ=255)
  aCGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aa                                                                                       >  2:3837621/1‑93 (MQ=255)
  aCGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aa                                                                                       <  2:3636801/93‑1 (MQ=255)
  aCGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGCCTTCCCTCAAG‑aa                                                                                       >  1:912307/1‑93 (MQ=255)
   cGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaa                                                                                      <  2:3022835/93‑1 (MQ=255)
        cACTGGTAGTCGGCTTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaatc                                                                                 <  2:368366/93‑4 (MQ=255)
        cACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaatc                                                                                 >  1:2181770/1‑90 (MQ=255)
         aCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaatca                                                                                >  2:1314259/1‑89 (MQ=255)
           tGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAgg                                                                               <  2:2767216/93‑1 (MQ=255)
           tGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAgg                                                                               >  1:2451827/1‑93 (MQ=255)
             gTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGcc                                                                             <  1:4469221/93‑1 (MQ=255)
               aGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAg                                                                           <  2:2660823/93‑1 (MQ=255)
               aGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAg                                                                           <  2:4415329/93‑1 (MQ=255)
                gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGc                                                                          >  1:3985208/1‑93 (MQ=255)
                 tCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCa                                                                         <  1:2295053/93‑1 (MQ=255)
                  cGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAt                                                                        <  2:1534162/93‑1 (MQ=255)
                  cGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAt                                                                        <  2:636105/93‑1 (MQ=255)
                  cGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAt                                                                        <  2:2161181/93‑1 (MQ=255)
                   ggCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAtt                                                                       <  1:2612852/93‑1 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGc                                                                    >  2:1771449/1‑93 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGc                                                                    >  2:881155/1‑93 (MQ=255)
                        gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATTAGGCCAGCATTTGCTg                                                                  <  2:1620183/93‑1 (MQ=255)
                          gCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGc                                                                >  2:2742042/1‑93 (MQ=255)
                          gCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGc                                                                >  2:4328490/1‑93 (MQ=255)
                          gCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGc                                                                >  1:506465/1‑93 (MQ=255)
                           ctgTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGcc                                                               >  2:4218510/3‑93 (MQ=255)
                              tCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCa                                                            >  2:1245774/1‑93 (MQ=255)
                                aTTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa                                                          <  2:4358145/93‑1 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt                                                         >  1:3499145/1‑93 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt                                                         <  2:1894340/93‑1 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt                                                         >  2:82078/1‑93 (MQ=255)
                                 ttGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAt                                                         >  1:1404300/1‑93 (MQ=255)
                                  tGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAtt                                                        <  2:2317362/93‑1 (MQ=255)
                                   gCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTg                                                       <  1:3957508/93‑1 (MQ=255)
                                     cGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGAt                                                     >  1:3840388/1‑93 (MQ=255)
                                       tCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                   >  2:941811/1‑93 (MQ=255)
                                       tCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCCGGCCCCAGATTGATaa                                                   >  1:2537747/1‑93 (MQ=255)
                                         aCTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACa                                                 <  2:708122/93‑1 (MQ=255)
                                          cTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa                                                <  1:2206194/93‑1 (MQ=255)
                                          cTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaa                                                >  2:3868259/1‑93 (MQ=255)
                                           tGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa                                               >  1:2110736/1‑93 (MQ=255)
                                           tGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa                                               >  2:1862537/1‑93 (MQ=255)
                                            gAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg                                              <  2:3130177/93‑1 (MQ=255)
                                             aaaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTCGCCCCAGATTGATAACAAAGt                                             <  2:2983556/93‑1 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                            >  2:2418426/1‑93 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                            <  2:552159/93‑1 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                            <  1:729246/93‑1 (MQ=255)
                                               aGTGCTGCGGCCAGCAGCAGTTTGCGCATCATAGTCTTCCCACAAGAAAAAAGCAGGCCAGCTTTTGCGGGCCCCAGATTGATAACAAAGTAc                                           <  2:3965481/93‑1 (MQ=255)
                                               aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc                                           <  2:1355832/93‑1 (MQ=255)
                                               aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc                                           <  2:320720/93‑1 (MQ=255)
                                               aGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCGCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAc                                           <  1:1074976/93‑1 (MQ=255)
                                                gTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcg                                          >  1:2602567/1‑93 (MQ=255)
                                                  gctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg                                        >  2:746908/1‑93 (MQ=255)
                                                  gctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg                                        >  2:3408717/1‑93 (MQ=255)
                                                   ctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGt                                       <  1:44029/93‑1 (MQ=255)
                                                    tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtt                                      <  1:4501654/93‑1 (MQ=255)
                                                    tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtt                                      <  2:2642431/93‑1 (MQ=255)
                                                      ccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                                    <  1:2247940/93‑1 (MQ=255)
                                                      ccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                                    >  1:1545337/1‑93 (MQ=255)
                                                       cgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                   >  2:1972857/1‑93 (MQ=255)
                                                       cgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTc                                   >  1:1653131/1‑93 (MQ=255)
                                                        gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                  >  2:1400355/1‑93 (MQ=255)
                                                         ccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCa                                 <  1:2005849/93‑1 (MQ=255)
                                                          cagcagCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAt                                <  2:115821/93‑1 (MQ=255)
                                                          cagcagCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAt                                >  1:2130312/1‑93 (MQ=255)
                                                          cagcagCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAt                                >  2:3524011/1‑93 (MQ=255)
                                                           agcagcATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATg                               >  1:1488137/1‑93 (MQ=255)
                                                            gcagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                              >  1:3219563/1‑93 (MQ=255)
                                                              agcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg                            >  2:1955074/1‑93 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                           >  2:1217482/1‑93 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                           >  1:836360/1‑93 (MQ=255)
                                                                caTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGa                          <  2:723077/93‑1 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                         >  1:4088233/1‑93 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                         <  1:2209110/93‑1 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                        >  1:1216714/1‑93 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                        >  2:3119488/1‑93 (MQ=255)
                                                                   tttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgc                       <  1:187396/93‑1 (MQ=255)
                                                                      gcgcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggc                    >  1:1728047/1‑93 (MQ=255)
                                                                      gcgcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggc                    <  2:3136940/93‑1 (MQ=255)
                                                                        gcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcgc                  >  1:2908139/1‑92 (MQ=255)
                                                                        gcATCATAGTCTTCCCTCAAGAAAAAATCAAGACAGCCTTTGCTGGCCCCAGATTGATAACAAAGCACGCGTTTTCCATGCCGGATgcggcgc                  >  2:1563068/1‑92 (MQ=37)
                                                                          atcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaa                >  1:277945/1‑93 (MQ=255)
                                                                          atcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaa                >  2:4637156/1‑93 (MQ=255)
                                                                          atcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaa                >  2:3316418/1‑93 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaaa               <  1:3482395/93‑1 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaaa               >  1:4340152/1‑93 (MQ=255)
                                                                             atAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAacc             >  1:4296404/1‑92 (MQ=38)
                                                                             atAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAaca             <  1:612946/93‑1 (MQ=38)
                                                                             atAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAaca             >  2:3484290/1‑93 (MQ=255)
                                                                               aGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAcc           <  2:4162023/93‑1 (MQ=38)
                                                                                  cTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGTTGCGGCGCAAACACCTTa        <  2:1117863/93‑1 (MQ=255)
                                                                                  cTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTa        <  2:267956/93‑1 (MQ=255)
                                                                                   ttCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAt       <  1:3534869/93‑1 (MQ=255)
                                                                                   ttCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAt       >  1:2698567/1‑93 (MQ=255)
                                                                                   ttCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAt       <  2:2458772/93‑1 (MQ=38)
                                                                                   ttCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAt       >  1:4201400/1‑93 (MQ=255)
                                                                                    tCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATc      <  2:2895706/93‑1 (MQ=255)
                                                                                      ccTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATccc    >  1:2380982/1‑93 (MQ=255)
                                                                                             |                                                                                       
TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC  >  NC_000913/3099564‑3099743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  >  NC_000913/3099568‑3099754
                                                                                              |                                                                                             
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAA                                                                                               <  SRR4302535.1941923/94‑1 (MQ=60)
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAA                                                                                               <  SRR4302535.1679620/94‑1 (MQ=60)
   GCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAatc                                                                                            >  SRR4302535.2197332/1‑91 (MQ=60)
   GCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAAatc                                                                                            <  SRR4302535.171200/94‑4 (MQ=60)
     ACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCGCAAGAAAAAATCAG                                                                                           <  SRR4302535.4501159/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGG                                                                                          >  SRR4302535.2469499/1‑94 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGG                                                                                          <  SRR4302535.1039926/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGG                                                                                          <  SRR4302535.3831723/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGG                                                                                          <  SRR4302535.441260/94‑1 (MQ=60)
         GTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCA                                                                                       <  SRR4302535.4499888/94‑1 (MQ=60)
          TAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAG                                                                                      <  SRR4302535.2811910/94‑1 (MQ=60)
           AGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGC                                                                                     >  SRR4302535.4012442/1‑94 (MQ=60)
             TCGGCGTGAGCGGTCATTGCTGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAT                                                                                   >  SRR4302535.4426567/1‑94 (MQ=60)
             TCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAT                                                                                   <  SRR4302535.4283329/94‑1 (MQ=60)
             TCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAT                                                                                   <  SRR4302535.2311469/94‑1 (MQ=60)
               GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTT                                                                                 <  SRR4302535.2631593/94‑1 (MQ=60)
                     AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGC                                                                           <  SRR4302535.2872936/94‑1 (MQ=60)
                     AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGC                                                                           >  SRR4302535.510004/1‑94 (MQ=60)
                        GGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCGTTTGCTGGCCCC                                                                        >  SRR4302535.3561793/1‑94 (MQ=60)
                        GGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCC                                                                        >  SRR4302535.4257335/1‑94 (MQ=60)
                         GTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCA                                                                       >  SRR4302535.1169671/1‑94 (MQ=60)
                         GTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCAAGCATTTGCTGGCCCCA                                                                       >  SRR4302535.697840/1‑94 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    >  SRR4302535.1414317/1‑94 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    >  SRR4302535.3523472/1‑94 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    >  SRR4302535.2355501/1‑94 (MQ=60)
                              TGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTG                                                                  <  SRR4302535.4151155/94‑1 (MQ=60)
                               GCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGA                                                                 <  SRR4302535.3984530/94‑1 (MQ=60)
                                CCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGAT                                                                >  SRR4302535.3866852/1‑94 (MQ=60)
                                CCGCCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTACCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGAT                                                                <  SRR4302535.4560727/94‑1 (MQ=60)
                                 CGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATA                                                               >  SRR4302535.403352/1‑94 (MQ=60)
                                  GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCCGGCCCCAGATTGATAA                                                              >  SRR4302535.2556027/1‑94 (MQ=60)
                                      CTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAA                                                          <  SRR4302535.2221908/94‑1 (MQ=60)
                                      CTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAA                                                          >  SRR4302535.2125961/1‑94 (MQ=60)
                                        TAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        <  SRR4302535.3169058/94‑1 (MQ=60)
                                        GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        <  SRR4302535.1289430/94‑1 (MQ=60)
                                          AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                      <  SRR4302535.734361/94‑1 (MQ=60)
                                          AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                      >  SRR4302535.3406102/1‑94 (MQ=60)
                                           AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                     >  SRR4302535.2621250/1‑94 (MQ=60)
                                           AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                     <  SRR4302535.2620714/94‑1 (MQ=60)
                                           AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCGCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                     <  SRR4302535.1082617/94‑1 (MQ=60)
                                           AGCGCTGCCGCGAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                     <  SRR4302535.1530946/94‑1 (MQ=60)
                                            GTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGC                                                    >  SRR4302535.2487161/1‑94 (MQ=60)
                                               CTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                 <  SRR4302535.44361/94‑1 (MQ=60)
                                               CTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                 >  SRR4302535.3517642/1‑94 (MQ=60)
                                                TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                >  SRR4302535.1635460/1‑94 (MQ=60)
                                                TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                <  SRR4302535.4532569/94‑1 (MQ=60)
                                                 GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                               >  SRR4302535.1556464/1‑94 (MQ=60)
                                                  CCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTC                                              <  SRR4302535.2263944/94‑1 (MQ=60)
                                                  CCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTC                                              >  SRR4302535.1665094/1‑94 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           >  SRR4302535.2145671/1‑94 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302535.2020297/94‑1 (MQ=60)
                                                     CCAGCAGCATTTGGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302535.225168/94‑1 (MQ=60)
                                                      CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          >  SRR4302535.1498805/1‑94 (MQ=60)
                                                      CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAAGCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          <  SRR4302535.3997642/94‑1 (MQ=60)
                                                       AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                         >  SRR4302535.3241871/1‑94 (MQ=60)
                                                         CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                       >  SRR4302535.982601/1‑94 (MQ=60)
                                                          AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                      >  SRR4302535.842228/1‑94 (MQ=60)
                                                          AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                      >  SRR4302535.1756233/1‑94 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    >  SRR4302535.4116206/1‑94 (MQ=60)
                                                             ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   <  SRR4302535.2224845/94‑1 (MQ=60)
                                                             ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   >  SRR4302535.1225439/1‑94 (MQ=60)
                                                               TTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCG                                 <  SRR4302535.188691/94‑1 (MQ=60)
                                                                TTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGG                                <  SRR4302535.2197873/94‑1 (MQ=60)
                                                                 TGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGC                               >  SRR4302535.1740537/1‑94 (MQ=60)
                                                                  GCGCATCATACTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCG                              <  SRR4302535.2465504/94‑1 (MQ=60)
                                                                   CGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGC                             >  SRR4302535.2928695/1‑94 (MQ=60)
                                                                     CATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAA                           >  SRR4302535.279883/1‑94 (MQ=60)
                                                                     CATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAA                           <  SRR4302535.2222787/94‑1 (MQ=60)
                                                                      ATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAA                          >  SRR4302535.2642499/1‑94 (MQ=60)
                                                                      ATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAA                          >  SRR4302535.4369838/1‑94 (MQ=60)
                                                                       TCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                         <  SRR4302535.3506578/94‑1 (MQ=60)
                                                                        CATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACC                        >  SRR4302535.4325805/1‑94 (MQ=60)
                                                                         ATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAC                       <  SRR4302535.617209/94‑1 (MQ=60)
                                                                         ATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAC                       >  SRR4302535.2738472/1‑94 (MQ=60)
                                                                          TAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACC                      <  SRR4302535.2273888/94‑1 (MQ=60)
                                                                          TAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACC                      >  SRR4302535.3748380/1‑94 (MQ=60)
                                                                          TAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACC                      <  SRR4302535.2151557/94‑1 (MQ=60)
                                                                            GTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTT                    >  SRR4302535.4573667/1‑94 (MQ=60)
                                                                            GTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTT                    >  SRR4302535.3970576/1‑94 (MQ=60)
                                                                             TCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTA                   >  SRR4302535.2626986/1‑94 (MQ=60)
                                                                             TCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTA                   <  SRR4302535.4229838/94‑1 (MQ=60)
                                                                             TCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTA                   <  SRR4302535.3536950/94‑1 (MQ=60)
                                                                              CTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  >  SRR4302535.210201/1‑94 (MQ=60)
                                                                              CTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  >  SRR4302535.2717852/1‑94 (MQ=60)
                                                                              CTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  >  SRR4302535.4230148/1‑94 (MQ=60)
                                                                              CTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302535.149779/94‑1 (MQ=60)
                                                                               TTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATC                 <  SRR4302535.3559407/94‑1 (MQ=60)
                                                                                 CCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC               <  SRR4302535.2206744/94‑1 (MQ=60)
                                                                                 CCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC               <  SRR4302535.552950/94‑1 (MQ=60)
                                                                                 CCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC               >  SRR4302535.2398081/1‑94 (MQ=60)
                                                                                  CCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCG              >  SRR4302535.891079/1‑94 (MQ=60)
                                                                                  CCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCG              <  SRR4302535.2128408/94‑1 (MQ=60)
                                                                                  CCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCG              >  SRR4302535.4352014/1‑94 (MQ=60)
                                                                                   CTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGC             >  SRR4302535.2399803/1‑94 (MQ=60)
                                                                                    TCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCC            >  SRR4302535.2105244/1‑94 (MQ=60)
                                                                                     CAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCT           <  SRR4302535.2641738/94‑1 (MQ=60)
                                                                                      AAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCGTA          >  SRR4302535.281013/1‑94 (MQ=60)
                                                                                      AAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTA          >  SRR4302535.659229/1‑94 (MQ=60)
                                                                                       agaAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTAC         >  SRR4302535.1117045/4‑94 (MQ=60)
                                                                                          AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCG      <  SRR4302535.2807345/94‑1 (MQ=60)
                                                                                             AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC   >  SRR4302535.4564317/1‑94 (MQ=60)
                                                                                             AATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC   >  SRR4302535.3962663/1‑94 (MQ=60)
                                                                                              ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCG  <  SRR4302535.1454097/94‑1 (MQ=60)
                                                                                              ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCG  <  SRR4302535.215191/94‑1 (MQ=60)
                                                                                              ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCG  >  SRR4302535.3477505/1‑94 (MQ=60)
                                                                                              |                                                                                             
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  >  NC_000913/3099568‑3099754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑