Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,099,661 (A)5→6 intergenic (‑19/+164) yggN ← / ← b2959 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,099,6561.A99.5% 1356.5 / ‑1.4 368intergenic (‑14/+169)yggN/b2959hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base A (172/194);  total (172/196)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

Only 100 of 399 total aligned reads displayed.

TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC  >  NC_000913/3099564‑3099743
                                                                                             |                                                                                       
tGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAg                                                                                          >  2:245087/1‑93 (MQ=255)
tGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAg                                                                                          <  1:2815995/93‑1 (MQ=255)
    gCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaa                                                                                     <  1:5507080/93‑1 (MQ=255)
     cTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaa                                                                                    >  1:4575813/1‑93 (MQ=255)
      tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaa                                                                                   >  1:1247069/1‑92 (MQ=255)
      tGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaa                                                                                   <  1:7529529/93‑2 (MQ=255)
       gCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaat                                                                                  >  2:7158239/1‑91 (MQ=255)
         aCTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑aaaaaatca                                                                                <  2:5425106/93‑5 (MQ=255)
           tGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAgg                                                                               <  2:1763139/93‑1 (MQ=255)
            ggTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGc                                                                              <  2:4790694/93‑1 (MQ=255)
            ggTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGc                                                                              <  1:7097874/93‑1 (MQ=255)
            ggTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGc                                                                              <  1:1644857/93‑1 (MQ=255)
              tAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCa                                                                            >  2:256480/1‑93 (MQ=255)
                gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGc                                                                          >  2:3360705/1‑93 (MQ=255)
                gTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGc                                                                          >  2:371823/1‑93 (MQ=255)
                  cGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAt                                                                        <  2:5881118/93‑1 (MQ=255)
                   ggCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAtt                                                                       <  2:6773492/93‑1 (MQ=255)
                    gCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCAttt                                                                      >  1:5439199/1‑93 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGc                                                                    <  1:2633052/93‑1 (MQ=255)
                      gTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGc                                                                    <  2:1416348/93‑1 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCt                                                                   >  2:3854466/1‑93 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCt                                                                   >  2:7340474/1‑93 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCt                                                                   >  1:6269407/1‑93 (MQ=255)
                       tGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCt                                                                   <  2:4333881/93‑1 (MQ=255)
                        gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTg                                                                  <  2:753499/93‑1 (MQ=255)
                        gAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTg                                                                  <  1:2325192/93‑1 (MQ=255)
                         aGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTgg                                                                 >  2:860293/1‑93 (MQ=255)
                          gCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGc                                                                >  1:6783138/1‑93 (MQ=255)
                          gCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGc                                                                >  1:6410543/1‑93 (MQ=255)
                          gCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGc                                                                >  1:1133220/1‑93 (MQ=255)
                            ggTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGccc                                                              <  1:6304621/93‑1 (MQ=255)
                               cATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAg                                                           <  1:2271759/93‑1 (MQ=255)
                                aTTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa                                                          >  2:5965085/1‑93 (MQ=255)
                                aTTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGa                                                          <  1:7889625/93‑1 (MQ=255)
                                    ccGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTCCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGa                                                      <  1:7399769/93‑1 (MQ=255)
                                     cGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGAt                                                     <  1:5217120/93‑1 (MQ=255)
                                       tCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATaa                                                   >  1:2964238/1‑93 (MQ=255)
                                        cACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAc                                                  <  2:5637531/93‑1 (MQ=255)
                                           tGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACaaa                                               >  2:2776088/1‑93 (MQ=255)
                                            gAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg                                              <  2:2686152/93‑1 (MQ=255)
                                            gAAAGTGCTGCCGCCAGCAGAATATTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAg                                              <  2:4297957/93‑1 (MQ=255)
                                             aaaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt                                             <  2:1243833/93‑1 (MQ=255)
                                             aaaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGt                                             <  2:7734515/93‑1 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                            >  1:6040811/1‑93 (MQ=255)
                                              aaGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTa                                            <  2:6238273/93‑1 (MQ=255)
                                               aGTGCTGCCGCCAGAAACATTTAAAGAATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTAGCTGGCCCCAGATTGATAACAAAGtcc                                           <  1:6999318/93‑3 (MQ=255)
                                                gTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcg                                          >  2:4404245/1‑93 (MQ=255)
                                                 tgctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgc                                         <  1:6583506/93‑1 (MQ=255)
                                                  gctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg                                        >  1:3662381/1‑93 (MQ=255)
                                                  gctgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAcgcg                                        <  2:1044489/93‑1 (MQ=255)
                                                    tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtt                                      >  2:2887468/1‑93 (MQ=255)
                                                    tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtt                                      >  1:4154453/1‑93 (MQ=255)
                                                    tgcCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtt                                      >  1:2043909/1‑93 (MQ=255)
                                                     gccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGttt                                     <  2:1193396/93‑1 (MQ=255)
                                                      ccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                                    <  1:4285352/93‑1 (MQ=255)
                                                      ccgccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGtttt                                    <  2:7863590/93‑1 (MQ=255)
                                                        gccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTcc                                  <  1:3061472/93‑1 (MQ=255)
                                                         cccgcagcaTGTTGCGGATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCa                                 <  1:612387/90‑1 (MQ=255)
                                                         ccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCa                                 <  2:901935/93‑1 (MQ=255)
                                                         ccAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCa                                 <  1:7710447/93‑1 (MQ=255)
                                                         ccAGAAGCATTTTAAGAATAATAGTATTCCATCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCa                                 <  1:1710434/93‑1 (MQ=255)
                                                            gcagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGc                              >  2:1539704/1‑93 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCTCAGATTGATAACAAAGTACGCGTTTTCCATGcc                             >  1:7601279/1‑93 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                             >  1:3748180/1‑93 (MQ=255)
                                                             cagcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGcc                             <  2:510917/93‑1 (MQ=255)
                                                              agcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCg                            >  1:1043187/1‑93 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTGCGCGTTTTCCATGCCgg                           >  1:5949462/1‑93 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                           >  2:1146166/1‑93 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                           <  2:5023547/93‑1 (MQ=255)
                                                               gcaTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCgg                           >  1:5094132/1‑93 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                         <  2:1113883/93‑1 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                         <  1:3989845/93‑1 (MQ=255)
                                                                 aTTTTGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAt                         <  1:4736404/93‑1 (MQ=255)
                                                                  ttttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATg                        <  2:2090389/93‑1 (MQ=255)
                                                                   tttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgc                       <  2:4023349/93‑1 (MQ=255)
                                                                   tttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgc                       <  2:3170734/93‑1 (MQ=255)
                                                                    ttGCGCATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcg                      <  1:3572591/93‑1 (MQ=255)
                                                                       cgcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcg                   >  1:5724322/1‑93 (MQ=255)
                                                                       cgcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcg                   >  2:3448281/1‑93 (MQ=255)
                                                                        gcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCGCCAGATTGCTCACACAGTACGCGTTTTCCGTGCCGGCTggggcgc                  >  1:2911951/1‑92 (MQ=255)
                                                                        gcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcgc                  >  2:5248621/1‑92 (MQ=255)
                                                                        gcATCATAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATgcggcgc                  >  2:3263353/1‑92 (MQ=255)
                                                                          atcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaa                >  1:3864635/1‑93 (MQ=255)
                                                                          atcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaa                >  2:7665924/1‑93 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaaa               >  2:518009/1‑93 (MQ=255)
                                                                           tcatAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaaa               >  1:2412665/1‑93 (MQ=255)
                                                                            catAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAac              <  1:1820952/93‑1 (MQ=255)
                                                                            catAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAac              <  1:3459539/93‑1 (MQ=255)
                                                                             atAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAaca             >  2:6526512/1‑93 (MQ=255)
                                                                              tAGTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAacac            >  2:7441712/1‑93 (MQ=255)
                                                                                gTCTTCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCt          <  2:6135642/93‑1 (MQ=38)
                                                                                   ttCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAt       >  2:2300915/1‑93 (MQ=255)
                                                                                    tCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATc      <  1:900737/93‑1 (MQ=38)
                                                                                    tCCCTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATc      <  2:4149792/93‑1 (MQ=38)
                                                                                     cccTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATcc     >  2:4742063/1‑93 (MQ=255)
                                                                                     cccTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATcc     >  2:6977369/1‑93 (MQ=255)
                                                                                     cccTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATcc     <  1:4181193/93‑1 (MQ=38)
                                                                                     cccTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATcc     >  1:4805/1‑93 (MQ=255)
                                                                                      ccTCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATccc    >  1:1470218/1‑93 (MQ=255)
                                                                                        tCAAGAAAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGc  >  2:6400466/1‑93 (MQ=255)
                                                                                             |                                                                                       
TGACGCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAAATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGC  >  NC_000913/3099564‑3099743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 217 total aligned reads displayed.
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  >  NC_000913/3099568‑3099754
                                                                                               |                                                                                             
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAA‑A                                                                                               >  SRR4302543.4617415/1‑94 (MQ=60)
 CTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAA‑AA                                                                                              >  SRR4302543.1258264/1‑94 (MQ=60)
  TGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAA‑Aat                                                                                             <  SRR4302543.7601351/94‑3 (MQ=60)
  TGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAA‑Aat                                                                                             >  SRR4302543.1975165/1‑92 (MQ=60)
  TGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAA‑Aat                                                                                             <  SRR4302543.7601350/94‑3 (MQ=60)
   GCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAA‑Aatc                                                                                            <  SRR4302543.7637549/94‑4 (MQ=60)
    CACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCA                                                                                            <  SRR4302543.4402624/94‑1 (MQ=60)
     ACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAG                                                                                           >  SRR4302543.428069/1‑94 (MQ=60)
     ACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAG                                                                                           >  SRR4302543.3327285/1‑94 (MQ=60)
     ACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAG                                                                                           >  SRR4302543.3327283/1‑94 (MQ=60)
     ACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAG                                                                                           <  SRR4302543.2429981/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGGGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGG                                                                                          <  SRR4302543.6839074/94‑1 (MQ=60)
      CTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGG                                                                                          <  SRR4302543.3646702/94‑1 (MQ=60)
        GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCC                                                                                        <  SRR4302543.7166796/94‑1 (MQ=60)
        GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCC                                                                                        <  SRR4302543.7166797/94‑1 (MQ=60)
        GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCC                                                                                        <  SRR4302543.1659547/94‑1 (MQ=60)
        GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCC                                                                                        >  SRR4302543.4669586/1‑94 (MQ=60)
        GGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCC                                                                                        <  SRR4302543.983740/94‑1 (MQ=60)
          GAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAG                                                                                      <  SRR4302543.2510754/94‑1 (MQ=60)
           AGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGC                                                                                     >  SRR4302543.3021166/1‑94 (MQ=60)
            GTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCA                                                                                    >  SRR4302543.4206554/1‑94 (MQ=60)
             TCGGCGTGAGCGGCCACTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCAT                                                                                   <  SRR4302543.2317415/94‑1 (MQ=60)
              CGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATT                                                                                  <  SRR4302543.4800346/94‑1 (MQ=60)
               GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTT                                                                                 >  SRR4302543.5488581/1‑94 (MQ=60)
               GGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTT                                                                                 <  SRR4302543.5361236/94‑1 (MQ=60)
                GCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTG                                                                                <  SRR4302543.6050033/94‑1 (MQ=60)
                GCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTG                                                                                <  SRR4302543.1129660/94‑1 (MQ=60)
                 CGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGC                                                                               <  SRR4302543.7714943/94‑1 (MQ=60)
                 CGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGC                                                                               >  SRR4302543.1216806/1‑94 (MQ=60)
                 CGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGC                                                                               >  SRR4302543.882770/1‑94 (MQ=60)
                  GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCT                                                                              <  SRR4302543.1500739/94‑1 (MQ=60)
                  GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCT                                                                              <  SRR4302543.2656891/94‑1 (MQ=60)
                  GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCT                                                                              <  SRR4302543.2329924/94‑1 (MQ=60)
                  GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCT                                                                              >  SRR4302543.3523421/1‑94 (MQ=60)
                  GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCT                                                                              <  SRR4302543.3468637/94‑1 (MQ=60)
                  GTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCT                                                                              >  SRR4302543.6328530/1‑94 (MQ=60)
                   TGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTG                                                                             <  SRR4302543.4076083/94‑1 (MQ=60)
                    GAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGG                                                                            <  SRR4302543.2346320/94‑1 (MQ=60)
                     AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGC                                                                           >  SRR4302543.1143512/1‑94 (MQ=60)
                     AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGC                                                                           >  SRR4302543.6847196/1‑94 (MQ=60)
                     AGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGC                                                                           >  SRR4302543.6470912/1‑94 (MQ=60)
                      GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCC                                                                          <  SRR4302543.6850376/94‑1 (MQ=60)
                      GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCC                                                                          <  SRR4302543.6839300/94‑1 (MQ=60)
                      GCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCC                                                                          >  SRR4302543.7977249/1‑94 (MQ=60)
                        GGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCC                                                                        <  SRR4302543.6989499/94‑1 (MQ=60)
                           CATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGA                                                                     <  SRR4302543.7281115/94‑1 (MQ=60)
                           CATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGA                                                                     <  SRR4302543.2292520/94‑1 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    >  SRR4302543.5491076/1‑94 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    <  SRR4302543.6599479/94‑1 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    <  SRR4302543.5241028/94‑1 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    <  SRR4302543.2002647/94‑1 (MQ=60)
                            ATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGAT                                                                    <  SRR4302543.7963832/94‑1 (MQ=60)
                             TTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATT                                                                   <  SRR4302543.1240537/94‑1 (MQ=60)
                             TTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATT                                                                   <  SRR4302543.5384627/94‑1 (MQ=60)
                             TTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATT                                                                   >  SRR4302543.6560637/1‑94 (MQ=60)
                              TGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTG                                                                  <  SRR4302543.2966678/94‑1 (MQ=60)
                                 CGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATA                                                               <  SRR4302543.5264116/94‑1 (MQ=60)
                                  GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                              >  SRR4302543.3070987/1‑94 (MQ=60)
                                  GTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAA                                                              >  SRR4302543.2991164/1‑94 (MQ=60)
                                   TCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAAC                                                             >  SRR4302543.3598608/1‑94 (MQ=60)
                                     ACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAA                                                           >  SRR4302543.4841189/1‑94 (MQ=60)
                                      CTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAA                                                          >  SRR4302543.817055/1‑94 (MQ=60)
                                      CTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAA                                                          >  SRR4302543.7238390/1‑94 (MQ=60)
                                        GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        <  SRR4302543.5549011/94‑1 (MQ=60)
                                        GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        <  SRR4302543.7560261/94‑1 (MQ=60)
                                        GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        <  SRR4302543.3883158/94‑1 (MQ=60)
                                        GAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGT                                                        <  SRR4302543.3883159/94‑1 (MQ=60)
                                         AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                       <  SRR4302543.4060176/94‑1 (MQ=60)
                                         AAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTA                                                       >  SRR4302543.7482833/1‑94 (MQ=60)
                                          AAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTAC                                                      <  SRR4302543.789486/94‑1 (MQ=60)
                                           AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                     <  SRR4302543.6366316/94‑1 (MQ=60)
                                           AGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACG                                                     >  SRR4302543.6110757/1‑94 (MQ=60)
                                           AGTGCTGCCGCCAGAAACATTTAAAGAATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTAGCTGGCCCCAGATTGATAACAAAGTCCG                                                     <  SRR4302543.7065544/94‑1 (MQ=57)
                                            GTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGC                                                    <  SRR4302543.6363648/94‑1 (MQ=60)
                                            GTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGC                                                    >  SRR4302543.1290851/1‑94 (MQ=60)
                                             TGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCG                                                   <  SRR4302543.3710447/94‑1 (MQ=60)
                                             TGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCG                                                   <  SRR4302543.6645510/94‑1 (MQ=60)
                                             TGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCG                                                   <  SRR4302543.4211907/94‑1 (MQ=60)
                                             TGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCG                                                   >  SRR4302543.3694545/1‑94 (MQ=60)
                                             GGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCG                                                   <  SRR4302543.4211908/94‑1 (MQ=60)
                                             GGCTGCCGCCAGCAGCATGTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCG                                                   <  SRR4302543.1804515/94‑1 (MQ=60)
                                               CTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                 >  SRR4302543.2062237/1‑94 (MQ=60)
                                               CTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTT                                                 >  SRR4302543.4192014/1‑94 (MQ=60)
                                                TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                <  SRR4302543.1677106/94‑1 (MQ=60)
                                                TGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTT                                                >  SRR4302543.2984037/1‑94 (MQ=60)
                                                 GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                               >  SRR4302543.5819913/1‑94 (MQ=60)
                                                 GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                               <  SRR4302543.6355653/94‑1 (MQ=60)
                                                 GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                               <  SRR4302543.3977285/94‑1 (MQ=60)
                                                 GCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTT                                               <  SRR4302543.6096711/94‑1 (MQ=60)
                                                  CCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTC                                              <  SRR4302543.4324295/94‑1 (MQ=60)
                                                   CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                             <  SRR4302543.1741890/94‑1 (MQ=60)
                                                   CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                             <  SRR4302543.567899/94‑1 (MQ=60)
                                                   CGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCC                                             >  SRR4302543.5807635/1‑94 (MQ=60)
                                                     ccagaagcattttaagaataatagtattccatcaagaAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302543.1725736/57‑1 (MQ=60)
                                                     CCCGCAGCATGTTGCGGATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302543.617986/94‑1 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302543.575397/94‑1 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302543.5501550/94‑1 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           >  SRR4302543.6593733/1‑94 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302543.6451882/94‑1 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           >  SRR4302543.2769435/1‑94 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302543.7851248/94‑1 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302543.720035/94‑1 (MQ=60)
                                                     CCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCAT                                           <  SRR4302543.3978756/94‑1 (MQ=60)
                                                      CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          <  SRR4302543.6061448/94‑1 (MQ=60)
                                                      CAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATG                                          >  SRR4302543.4959219/1‑94 (MQ=60)
                                                       AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                         >  SRR4302543.5802189/1‑94 (MQ=60)
                                                       AGCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGC                                         >  SRR4302543.3029186/1‑94 (MQ=60)
                                                        GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCTCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                        >  SRR4302543.7673473/1‑94 (MQ=60)
                                                        GCAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCC                                        >  SRR4302543.3781158/1‑94 (MQ=60)
                                                         CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                       >  SRR4302543.1052491/1‑94 (MQ=60)
                                                         CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                       >  SRR4302543.3636451/1‑94 (MQ=60)
                                                         CAGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCG                                       >  SRR4302543.4316763/1‑94 (MQ=60)
                                                          AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCCGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                      >  SRR4302543.7682267/1‑94 (MQ=60)
                                                          AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGCTTGATAACAAAGTACGCGCTTTCCATGCCGG                                      >  SRR4302543.1205074/1‑94 (MQ=60)
                                                          AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTGCGCGTTTTCCATGCCGG                                      >  SRR4302543.6003909/1‑94 (MQ=60)
                                                          AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                      >  SRR4302543.5140274/1‑94 (MQ=60)
                                                          AGCATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGG                                      >  SRR4302543.3767770/1‑94 (MQ=60)
                                                           ACATTTGGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGA                                     <  SRR4302543.4916422/94‑1 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    <  SRR4302543.978686/94‑1 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    >  SRR4302543.1284170/1‑94 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    <  SRR4302543.3770119/94‑1 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    >  SRR4302543.7671893/1‑94 (MQ=60)
                                                            CATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGAT                                    >  SRR4302543.267609/1‑94 (MQ=60)
                                                             ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   <  SRR4302543.4779691/94‑1 (MQ=60)
                                                             ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   <  SRR4302543.4025314/94‑1 (MQ=60)
                                                             ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   >  SRR4302543.2180644/1‑94 (MQ=60)
                                                             ATTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   >  SRR4302543.7779572/1‑94 (MQ=60)
                                                             ATTTTGCGCATCATAGTCTCCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATG                                   <  SRR4302543.2912163/94‑1 (MQ=60)
                                                              TTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGC                                  <  SRR4302543.6325963/94‑1 (MQ=60)
                                                              TTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGC                                  <  SRR4302543.6325962/94‑1 (MQ=60)
                                                              TTTTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGC                                  <  SRR4302543.4578948/94‑1 (MQ=60)
                                                                TTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGG                                >  SRR4302543.4823818/1‑94 (MQ=60)
                                                                TTGCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGG                                <  SRR4302543.3604176/94‑1 (MQ=60)
                                                                  GCGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCG                              >  SRR4302543.5776556/1‑94 (MQ=60)
                                                                   CGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCGCCAGATTGCTCACACAGTACGCGTTTTCCGTGCCGGctggggcgc                             >  SRR4302543.2938416/1‑85 (MQ=60)
                                                                   CGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGC                             >  SRR4302543.2938415/1‑94 (MQ=60)
                                                                   CGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGC                             >  SRR4302543.3268413/1‑94 (MQ=60)
                                                                   CGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGC                             >  SRR4302543.3268412/1‑94 (MQ=60)
                                                                    GCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCA                            <  SRR4302543.5645104/94‑1 (MQ=60)
                                                                     CATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAA                           >  SRR4302543.3898772/1‑94 (MQ=60)
                                                                      ATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAA                          >  SRR4302543.27737/1‑94 (MQ=60)
                                                                      ATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAA                          >  SRR4302543.2434468/1‑94 (MQ=60)
                                                                      ATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAA                          >  SRR4302543.1689442/1‑94 (MQ=60)
                                                                       TCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                         <  SRR4302543.1650018/94‑1 (MQ=60)
                                                                       TCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                         >  SRR4302543.2166822/1‑94 (MQ=60)
                                                                       TCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                         <  SRR4302543.158200/94‑1 (MQ=60)
                                                                       TCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                         <  SRR4302543.4276466/94‑1 (MQ=60)
                                                                        CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                        >  SRR4302543.7801643/1‑94 (MQ=60)
                                                                        CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                        <  SRR4302543.1837288/94‑1 (MQ=60)
                                                                        CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                        <  SRR4302543.7595774/94‑1 (MQ=60)
                                                                        CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                        <  SRR4302543.3574618/94‑1 (MQ=60)
                                                                        CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                        <  SRR4302543.3490534/94‑1 (MQ=60)
                                                                        CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                        >  SRR4302543.5252356/1‑94 (MQ=60)
                                                                         ATAGTCTTCCCTCAAGAAAAA‑ATCATGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGATTTCCATGCCGGATGCGGCGCAAACAC                       >  SRR4302543.4666748/1‑94 (MQ=60)
                                                                         ATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAC                       >  SRR4302543.2300488/1‑94 (MQ=60)
                                                                         ATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAC                       <  SRR4302543.2551660/94‑1 (MQ=60)
                                                                          TAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACC                      >  SRR4302543.4535069/1‑94 (MQ=60)
                                                                          TAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATCTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACC                      <  SRR4302543.3854750/94‑1 (MQ=60)
                                                                            GTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTT                    <  SRR4302543.2410002/94‑1 (MQ=60)
                                                                            GTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTT                    <  SRR4302543.2460008/94‑1 (MQ=60)
                                                                              CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302543.2989929/94‑1 (MQ=60)
                                                                              CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  >  SRR4302543.6357620/1‑94 (MQ=60)
                                                                              CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302543.6263682/94‑1 (MQ=60)
                                                                              CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302543.7084749/94‑1 (MQ=60)
                                                                              CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302543.3650777/94‑1 (MQ=60)
                                                                              CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  >  SRR4302543.5319794/1‑94 (MQ=60)
                                                                              CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  >  SRR4302543.1136620/1‑94 (MQ=60)
                                                                              CTTCCCTCAAGAAAAA‑ATCAGGCCAGCAGTTGCTGGCCCCATATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                  <  SRR4302543.4163025/94‑1 (MQ=60)
                                                                               TTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATC                 >  SRR4302543.6879740/1‑94 (MQ=60)
                                                                                TCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCC                >  SRR4302543.16967/1‑94 (MQ=60)
                                                                                TCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCC                <  SRR4302543.908684/94‑1 (MQ=60)
                                                                                TCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCC                >  SRR4302543.4849/1‑94 (MQ=60)
                                                                                TCCCTCAAG‑AAAACATCAGGCCGGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCC                <  SRR4302543.7080810/94‑1 (MQ=60)
                                                                                 CCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC               <  SRR4302543.6128145/94‑1 (MQ=60)
                                                                                 CCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC               >  SRR4302543.1483459/1‑94 (MQ=60)
                                                                                 CCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC               <  SRR4302543.4219082/94‑1 (MQ=60)
                                                                                 CCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC               >  SRR4302543.6721123/1‑94 (MQ=60)
                                                                                  CCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCG              >  SRR4302543.212168/1‑94 (MQ=60)
                                                                                    TCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCC            >  SRR4302543.983099/1‑94 (MQ=60)
                                                                                     CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCT           <  SRR4302543.5630588/94‑1 (MQ=60)
                                                                                     CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCT           <  SRR4302543.4585497/94‑1 (MQ=60)
                                                                                     CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCT           <  SRR4302543.4585498/94‑1 (MQ=60)
                                                                                     CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCT           >  SRR4302543.4933704/1‑94 (MQ=60)
                                                                                     CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGAACGCGTTTTCAATGCCGGATGCGGCGCACACAACTTATCGCGCCT           >  SRR4302543.4933705/1‑94 (MQ=60)
                                                                                       agaAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTAC         >  SRR4302543.2227608/4‑94 (MQ=60)
                                                                                       agaAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTAC         <  SRR4302543.5241128/91‑1 (MQ=60)
                                                                                        gaAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACT        <  SRR4302543.7632062/92‑1 (MQ=60)
                                                                                        A‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCTCAAACACCTTATCCCGCCTACTC       <  SRR4302543.3317291/94‑1 (MQ=60)
                                                                                        A‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTC       <  SRR4302543.2697707/94‑1 (MQ=60)
                                                                                        A‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTC       <  SRR4302543.1471723/94‑1 (MQ=60)
                                                                                          AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCG      <  SRR4302543.2741716/94‑1 (MQ=60)
                                                                                           AAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGA     >  SRR4302543.3477252/1‑94 (MQ=60)
                                                                                             A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC   <  SRR4302543.6838016/94‑1 (MQ=60)
                                                                                             A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC   <  SRR4302543.6751785/94‑1 (MQ=60)
                                                                                             A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC   <  SRR4302543.537974/94‑1 (MQ=60)
                                                                                             A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC   <  SRR4302543.3062297/94‑1 (MQ=60)
                                                                                             A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC   >  SRR4302543.1001970/1‑94 (MQ=60)
                                                                                             A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCAATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC   <  SRR4302543.4141422/94‑1 (MQ=60)
                                                                                             A‑ATCAGGCAAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC   <  SRR4302543.6258333/94‑1 (MQ=60)
                                                                                               ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCG  <  SRR4302543.4424113/94‑1 (MQ=60)
                                                                                               |                                                                                             
GCTGCACTGGTAGTCGGCGTGAGCGGTCATTGCCGTCACTGAAAGTGCTGCCGCCAGCAGCATTTTGCGCATCATAGTCTTCCCTCAAG‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCG  >  NC_000913/3099568‑3099754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑