Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,099,727 T→C intergenic (‑85/+98) yggN ← / ← b2959 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,099,7270TC99.2% 1137.1 / ‑2.4 364intergenic (‑85/+98)yggN/b2959hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base C (174/187);  total (177/187)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

Only 100 of 381 total aligned reads displayed.

ATCATAGTCTTCCCTCAAG‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGT  >  NC_000913/3099638‑3099816
                                                                                           |                                                                                         
atcatAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaa                                                                                         <  2:6785647/93‑1 (MQ=255)
atcatAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaa                                                                                         >  1:3864635/1‑93 (MQ=255)
atcatAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaa                                                                                         >  2:7665924/1‑93 (MQ=255)
 tcatAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaaa                                                                                        <  1:1635432/93‑1 (MQ=255)
 tcatAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaaa                                                                                        >  1:4181633/1‑93 (MQ=255)
 tcatAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCaaa                                                                                        <  1:4238022/93‑1 (MQ=255)
  catAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAac                                                                                       >  1:2147595/1‑93 (MQ=255)
   atAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAaca                                                                                      >  2:6526512/1‑93 (MQ=255)
    tAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAacac                                                                                     >  2:2304176/1‑93 (MQ=255)
    tAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAacac                                                                                     >  2:7441712/1‑93 (MQ=255)
     aGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAcc                                                                                    >  1:4494222/1‑93 (MQ=255)
     aGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAcc                                                                                    <  2:1137688/93‑1 (MQ=255)
      gTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCt                                                                                   <  2:6135642/93‑1 (MQ=38)
        cTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTa                                                                                 <  1:3618885/93‑1 (MQ=255)
         ttCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGGCGGATGCGGCGCAAACACCTTAt                                                                                >  2:4307879/1‑93 (MQ=255)
         ttCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAt                                                                                >  1:6298343/1‑93 (MQ=255)
         ttCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAt                                                                                >  1:1126400/1‑93 (MQ=255)
          tCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATc                                                                               <  2:4149793/93‑1 (MQ=255)
          tCCCTCAAG‑AAAACATCAGGCCGGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATc                                                                               <  1:7013865/93‑1 (MQ=38)
           cccTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATcc                                                                              <  1:6070613/93‑1 (MQ=255)
            ccTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATccc                                                                             >  2:2985740/1‑93 (MQ=255)
            ccTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATccc                                                                             <  2:4763794/93‑1 (MQ=38)
             cTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCg                                                                            >  2:4788096/1‑93 (MQ=255)
              tCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGc                                                                           >  2:3787706/1‑93 (MQ=255)
                aagaaaaa‑aTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCt                                                                         >  1:4889136/5‑93 (MQ=255)
                  gaaaaa‑aTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTAc                                                                       <  1:7560095/91‑1 (MQ=255)
                  gaaaaa‑aTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTAc                                                                       <  2:7848815/91‑1 (MQ=255)
                   aaaaa‑aTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACt                                                                      <  1:1458565/92‑1 (MQ=255)
                   aaaaa‑aTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACt                                                                      >  2:6944215/2‑93 (MQ=255)
                    aaaa‑aTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTc                                                                     <  2:4555348/93‑1 (MQ=255)
                       a‑aTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGAt                                                                  <  2:3112381/93‑1 (MQ=255)
                       a‑aTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGAt                                                                  <  2:4901273/93‑1 (MQ=255)
                       a‑aTCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCCACTCGAt                                                                  >  2:5668409/1‑93 (MQ=255)
                       a‑aTCAGGCAAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGAt                                                                  <  1:6199772/93‑1 (MQ=255)
                             ggCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGc                                                             >  1:6567539/1‑93 (MQ=255)
                              gCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCa                                                            <  1:2041749/93‑1 (MQ=255)
                                cAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATc                                                          >  1:7211857/1‑93 (MQ=255)
                                cAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATc                                                          <  1:6680521/93‑1 (MQ=255)
                                 aGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCt                                                         <  1:991531/93‑1 (MQ=255)
                                 aGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCt                                                         >  2:4240885/1‑93 (MQ=255)
                                    aTTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTc                                                      <  1:233377/93‑1 (MQ=255)
                                     tttGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTcc                                                     >  2:2480918/1‑93 (MQ=255)
                                     tttGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTcc                                                     <  2:6053947/93‑1 (MQ=255)
                                     tttGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTcc                                                     >  1:5923470/1‑93 (MQ=255)
                                      ttGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCa                                                    <  2:3565138/93‑1 (MQ=255)
                                        gCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAgg                                                  >  1:5709541/1‑93 (MQ=255)
                                         cTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGc                                                 <  1:5114578/93‑1 (MQ=255)
                                          tGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGcc                                                >  2:5257588/1‑93 (MQ=255)
                                          tGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGcc                                                <  2:2357341/93‑1 (MQ=255)
                                              cccAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGAt                                            >  1:2486270/1‑93 (MQ=255)
                                               ccAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATa                                           <  1:5316308/93‑1 (MQ=255)
                                               ccAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATa                                           <  1:444973/93‑1 (MQ=255)
                                               ccAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATa                                           >  1:3982526/1‑93 (MQ=255)
                                               ccAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATa                                           <  2:6226947/93‑1 (MQ=255)
                                                 aGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAg                                         >  1:4701978/1‑93 (MQ=255)
                                                 aGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAg                                         <  1:3101209/93‑1 (MQ=255)
                                                  gATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGc                                        >  1:1904219/1‑93 (MQ=255)
                                                   aTTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCg                                       >  2:3935050/1‑93 (MQ=255)
                                                   aTTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCg                                       >  2:828571/1‑93 (MQ=255)
                                                    ttGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGt                                      >  2:1239414/1‑93 (MQ=255)
                                                       aTAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAgc                                   <  2:2095728/93‑1 (MQ=255)
                                                       aTAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAgc                                   >  2:5005131/1‑93 (MQ=255)
                                                       aTAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAgc                                   <  1:6863254/93‑1 (MQ=255)
                                                        tAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAgcg                                  >  2:4805032/1‑93 (MQ=255)
                                                        tAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAgcg                                  >  1:5024335/1‑93 (MQ=255)
                                                          aCAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCa                                >  1:1011291/1‑93 (MQ=255)
                                                          aCAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCa                                <  2:5858458/93‑1 (MQ=255)
                                                           cAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATACGCGTAGCGCAt                               >  1:2418841/1‑93 (MQ=255)
                                                            aaaGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATc                              <  1:4592763/93‑1 (MQ=255)
                                                             aaGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCa                             >  1:4085026/1‑93 (MQ=255)
                                                              aGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAg                            <  2:2640556/93‑1 (MQ=255)
                                                               gTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAgg                           <  2:7339512/93‑1 (MQ=255)
                                                                tACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGc                          <  1:2225870/93‑1 (MQ=255)
                                                                 aCGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCa                         <  2:5117080/93‑1 (MQ=255)
                                                                  cgcgTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAg                        >  2:6307525/1‑93 (MQ=255)
                                                                   gctttttCCATGCCGGATGCGCCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGt                       <  1:2884054/90‑1 (MQ=255)
                                                                   gcgTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGt                       >  1:4124325/1‑93 (MQ=255)
                                                                     gTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGttt                     <  1:925095/93‑1 (MQ=255)
                                                                      ttttCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGtttt                    <  1:2726098/93‑1 (MQ=255)
                                                                      ttttCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGtttt                    >  1:7330102/1‑93 (MQ=255)
                                                                      ttttCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGtttt                    >  2:5358506/1‑93 (MQ=255)
                                                                      ttttCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGAGCATCAGGCAGtttt                    >  1:3445355/1‑93 (MQ=255)
                                                                      ttttCAATGCCGGATGCGGCGCAAACCCCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGtttt                    <  2:4034949/93‑1 (MQ=255)
                                                                          ccATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCAt                <  1:2774641/93‑1 (MQ=255)
                                                                           cATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCAtt               >  1:5387127/1‑93 (MQ=255)
                                                                            aTGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCAttt              >  2:1628844/1‑93 (MQ=255)
                                                                            aTGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCAttt              <  1:518009/93‑1 (MQ=255)
                                                                             tGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTg             >  1:6331633/1‑93 (MQ=255)
                                                                               ccGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTc           <  1:7910824/93‑1 (MQ=255)
                                                                               ccGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTc           <  2:443669/93‑1 (MQ=255)
                                                                                 ggATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCAt         <  1:2668964/93‑1 (MQ=255)
                                                                                 ggATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCAt         >  2:1828752/1‑93 (MQ=255)
                                                                                  gATGCGGCGCAAACCCCTTATCCCGCCTTCTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATc        <  1:7856330/93‑1 (MQ=255)
                                                                                  gATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATc        <  1:169267/93‑1 (MQ=255)
                                                                                    tGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGc      >  2:2796425/1‑93 (MQ=255)
                                                                                    tGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGc      >  1:6586723/1‑93 (MQ=255)
                                                                                     gcggcgCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGcc     >  1:3504028/1‑93 (MQ=255)
                                                                                      cggcgCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGccc    <  2:7502257/93‑1 (MQ=255)
                                                                                      cggcgCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGccc    <  2:3704738/93‑1 (MQ=255)
                                                                                       ggcgCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCg   >  2:3256355/1‑93 (MQ=255)
                                                                                           |                                                                                         
ATCATAGTCTTCCCTCAAG‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGT  >  NC_000913/3099638‑3099816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 226 total aligned reads displayed.
CGCATCATAGTCTTCCCTCAAG‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGC  >  NC_000913/3099635‑3099820
                                                                                              |                                                                                             
CGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGC                                                                                               >  SRR4302543.2938415/1‑94 (MQ=60)
CGCATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGC                                                                                               >  SRR4302543.3268412/1‑94 (MQ=60)
   ATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAA                                                                                            >  SRR4302543.2434468/1‑94 (MQ=60)
   ATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAA                                                                                            >  SRR4302543.1689442/1‑94 (MQ=60)
   ATCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAA                                                                                            >  SRR4302543.27737/1‑94 (MQ=60)
    TCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                                                                                           <  SRR4302543.4276466/94‑1 (MQ=60)
    TCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                                                                                           >  SRR4302543.2166822/1‑94 (MQ=60)
    TCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                                                                                           <  SRR4302543.1650018/94‑1 (MQ=60)
    TCATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAAC                                                                                           <  SRR4302543.158200/94‑1 (MQ=60)
     CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACC                                                                                          >  SRR4302543.3965046/1‑94 (MQ=60)
     CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                                                                                          >  SRR4302543.5252356/1‑94 (MQ=60)
     CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                                                                                          <  SRR4302543.3490534/94‑1 (MQ=60)
     CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                                                                                          <  SRR4302543.1837288/94‑1 (MQ=60)
     CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                                                                                          <  SRR4302543.3574618/94‑1 (MQ=60)
     CATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACA                                                                                          >  SRR4302543.7801643/1‑94 (MQ=60)
      ATAGTCTTCCCTCAAGAAAAA‑ATCATGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGATTTCCATGCCGGATGCGGCGCAAACAC                                                                                         >  SRR4302543.4666748/1‑94 (MQ=60)
      ATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAC                                                                                         >  SRR4302543.2300488/1‑94 (MQ=60)
      ATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAC                                                                                         <  SRR4302543.2551660/94‑1 (MQ=60)
      ATAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACAC                                                                                         >  SRR4302543.4666747/1‑94 (MQ=60)
       TAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACC                                                                                        >  SRR4302543.4535069/1‑94 (MQ=60)
       TAGTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATCTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACC                                                                                        <  SRR4302543.3854750/94‑1 (MQ=60)
         GTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTT                                                                                      <  SRR4302543.2873599/94‑1 (MQ=60)
         GTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTT                                                                                      >  SRR4302543.1948816/1‑94 (MQ=60)
         GTCTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTT                                                                                      <  SRR4302543.2410002/94‑1 (MQ=60)
           CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                                                                                    <  SRR4302543.2989929/94‑1 (MQ=60)
           CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                                                                                    <  SRR4302543.3650777/94‑1 (MQ=60)
           CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                                                                                    >  SRR4302543.5319794/1‑94 (MQ=60)
           CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                                                                                    <  SRR4302543.6263682/94‑1 (MQ=60)
           CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                                                                                    <  SRR4302543.7084749/94‑1 (MQ=60)
           CTTCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                                                                                    >  SRR4302543.1136620/1‑94 (MQ=60)
           CTTCCCTCAAGAAAAA‑ATCAGGCCAGCAGTTGCTGGCCCCATATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTAT                                                                                    <  SRR4302543.4163025/94‑1 (MQ=60)
             TCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCC                                                                                  >  SRR4302543.16967/1‑94 (MQ=60)
             TCCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCC                                                                                  <  SRR4302543.908684/94‑1 (MQ=60)
             TCCCTCAAG‑AAAACATCAGGCCGGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCC                                                                                  <  SRR4302543.7080810/94‑1 (MQ=60)
              CCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC                                                                                 >  SRR4302543.6721123/1‑94 (MQ=60)
              CCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC                                                                                 >  SRR4302543.1483459/1‑94 (MQ=60)
              CCCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCC                                                                                 <  SRR4302543.4219082/94‑1 (MQ=60)
               CCTCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCG                                                                                >  SRR4302543.212168/1‑94 (MQ=60)
                cctcaataaaaaaccagtCCAGCAGTTGCTGGCCCCAGATGGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCG                                                                                <  SRR4302543.1914693/76‑1 (MQ=60)
                 TCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCC                                                                              >  SRR4302543.983099/1‑94 (MQ=60)
                 TCAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCC                                                                              <  SRR4302543.5165757/94‑1 (MQ=60)
                  CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCT                                                                             <  SRR4302543.4585498/94‑1 (MQ=60)
                  CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCT                                                                             >  SRR4302543.4933704/1‑94 (MQ=60)
                  CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCT                                                                             <  SRR4302543.4585497/94‑1 (MQ=60)
                  CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCT                                                                             <  SRR4302543.5630588/94‑1 (MQ=60)
                  CAAGAAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGAACGCGTTTTCAATGCCGGATGCGGCGCACACAACTTATCGCGCCT                                                                             >  SRR4302543.4933705/1‑94 (MQ=60)
                    agaAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTAC                                                                           >  SRR4302543.2227608/4‑94 (MQ=60)
                    agaAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTAC                                                                           <  SRR4302543.5241128/91‑1 (MQ=60)
                     gaAAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACT                                                                          <  SRR4302543.7632062/92‑1 (MQ=60)
                     A‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCTCAAACACCTTATCCCGCCTACTC                                                                         <  SRR4302543.3317291/94‑1 (MQ=60)
                     A‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTC                                                                         <  SRR4302543.2697707/94‑1 (MQ=60)
                     A‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTC                                                                         <  SRR4302543.1471723/94‑1 (MQ=60)
                        AAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGA                                                                       >  SRR4302543.3477252/1‑94 (MQ=60)
                          A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC                                                                     >  SRR4302543.1001970/1‑94 (MQ=60)
                          A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC                                                                     <  SRR4302543.6838016/94‑1 (MQ=60)
                          A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC                                                                     <  SRR4302543.6751785/94‑1 (MQ=60)
                          A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC                                                                     <  SRR4302543.537974/94‑1 (MQ=60)
                          A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC                                                                     <  SRR4302543.3062297/94‑1 (MQ=60)
                          A‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCAATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC                                                                     <  SRR4302543.4141422/94‑1 (MQ=60)
                          A‑ATCAGGCAAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATC                                                                     <  SRR4302543.6258333/94‑1 (MQ=60)
                            ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCG                                                                    <  SRR4302543.4424113/94‑1 (MQ=60)
                              CAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTG                                                                  <  SRR4302543.5078395/94‑1 (MQ=60)
                               AGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGC                                                                 >  SRR4302543.4535641/1‑94 (MQ=60)
                               AGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGC                                                                 >  SRR4302543.6629353/1‑94 (MQ=60)
                               AGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGC                                                                 >  SRR4302543.4175508/1‑94 (MQ=60)
                                 GCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCAT                                                               <  SRR4302543.6169827/94‑1 (MQ=60)
                                 GCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCAT                                                               <  SRR4302543.500162/94‑1 (MQ=60)
                                  CCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATC                                                              >  SRR4302543.7281766/1‑94 (MQ=60)
                                  CCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATC                                                              >  SRR4302543.2337393/1‑94 (MQ=60)
                                   CAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCT                                                             <  SRR4302543.5945973/94‑1 (MQ=60)
                                   CAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCT                                                             >  SRR4302543.5384804/1‑94 (MQ=60)
                                   CAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCT                                                             >  SRR4302543.7591603/1‑94 (MQ=60)
                                   CAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCT                                                             <  SRR4302543.6743591/94‑1 (MQ=60)
                                    AGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTT                                                            <  SRR4302543.1000284/94‑1 (MQ=60)
                                    AGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTT                                                            <  SRR4302543.2395622/94‑1 (MQ=60)
                                    AGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGAGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTT                                                            >  SRR4302543.2537492/1‑94 (MQ=60)
                                     GCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTT                                                           >  SRR4302543.6003734/1‑94 (MQ=60)
                                     GCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTT                                                           >  SRR4302543.6003733/1‑94 (MQ=60)
                                       ATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCC                                                         >  SRR4302543.2278848/1‑94 (MQ=60)
                                       ATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCC                                                         >  SRR4302543.5977597/1‑94 (MQ=60)
                                       ATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCC                                                         <  SRR4302543.235391/94‑1 (MQ=60)
                                        TTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCA                                                        >  SRR4302543.6370473/1‑94 (MQ=60)
                                          TGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGG                                                      >  SRR4302543.738839/1‑94 (MQ=60)
                                          TGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGG                                                      <  SRR4302543.484817/94‑1 (MQ=60)
                                          TGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGG                                                      >  SRR4302543.5761630/1‑94 (MQ=60)
                                           GCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGC                                                     >  SRR4302543.1065847/1‑94 (MQ=60)
                                           GCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGC                                                     >  SRR4302543.4777371/1‑94 (MQ=60)
                                            CTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCGGCCTACTCGATCGTGCATCTTTCCAGGCC                                                    >  SRR4302543.6693228/1‑94 (MQ=60)
                                            CTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCC                                                    <  SRR4302543.1900322/94‑1 (MQ=60)
                                             TGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCT                                                   <  SRR4302543.3500714/94‑1 (MQ=60)
                                               GCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGA                                                 >  SRR4302543.3978534/1‑94 (MQ=60)
                                                CCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCTAGGCCTGAT                                                >  SRR4302543.3687634/1‑94 (MQ=60)
                                                CCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGAT                                                <  SRR4302543.2838996/94‑1 (MQ=60)
                                                CCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGAT                                                >  SRR4302543.6575705/1‑94 (MQ=60)
                                                CCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGAT                                                <  SRR4302543.4267902/94‑1 (MQ=60)
                                                CCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGAT                                                >  SRR4302543.2508789/1‑94 (MQ=60)
                                                 CCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATA                                               >  SRR4302543.647265/1‑94 (MQ=60)
                                                 CCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATA                                               <  SRR4302543.4141503/94‑1 (MQ=60)
                                                 CCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATA                                               >  SRR4302543.4017852/1‑94 (MQ=60)
                                                  CCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAA                                              >  SRR4302543.916384/1‑94 (MQ=60)
                                                  CCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAA                                              <  SRR4302543.449141/94‑1 (MQ=60)
                                                   CAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAG                                             <  SRR4302543.1332790/94‑1 (MQ=60)
                                                   CAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAG                                             >  SRR4302543.6639591/1‑94 (MQ=60)
                                                   CAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAG                                             >  SRR4302543.4744929/1‑94 (MQ=60)
                                                   CAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAG                                             <  SRR4302543.2579850/94‑1 (MQ=60)
                                                    AGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGC                                            >  SRR4302543.7703370/1‑94 (MQ=60)
                                                    AGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGC                                            <  SRR4302543.3129105/94‑1 (MQ=60)
                                                    AGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGC                                            >  SRR4302543.1921358/1‑94 (MQ=60)
                                                     GATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCACACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCG                                           >  SRR4302543.6379342/1‑94 (MQ=60)
                                                     GATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAGGCG                                           <  SRR4302543.7012611/94‑1 (MQ=60)
                                                     GATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCG                                           <  SRR4302543.6349495/94‑1 (MQ=60)
                                                     GATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCG                                           >  SRR4302543.3612241/1‑94 (MQ=60)
                                                     GATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCG                                           <  SRR4302543.1221125/94‑1 (MQ=60)
                                                      ATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGT                                          <  SRR4302543.955164/94‑1 (MQ=60)
                                                      ATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGT                                          <  SRR4302543.2238463/94‑1 (MQ=60)
                                                        TGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAG                                        <  SRR4302543.7034722/94‑1 (MQ=60)
                                                        TGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAG                                        <  SRR4302543.3865235/94‑1 (MQ=60)
                                                        TGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAG                                        <  SRR4302543.4043098/94‑1 (MQ=60)
                                                         GATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGC                                       >  SRR4302543.51630/1‑94 (MQ=60)
                                                         GATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGC                                       >  SRR4302543.1764159/1‑94 (MQ=60)
                                                          ATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCG                                      <  SRR4302543.6927922/94‑1 (MQ=60)
                                                          ATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCG                                      >  SRR4302543.5070056/1‑94 (MQ=60)
                                                          ATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCG                                      <  SRR4302543.325545/94‑1 (MQ=60)
                                                            AACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCA                                    >  SRR4302543.1020239/1‑94 (MQ=60)
                                                            AACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCA                                    >  SRR4302543.273498/1‑94 (MQ=60)
                                                            AACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCA                                    >  SRR4302543.5556425/1‑94 (MQ=60)
                                                             ACAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATACGCGTAGCGCAT                                   >  SRR4302543.2440685/1‑94 (MQ=60)
                                                              CAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACTCCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATC                                  <  SRR4302543.2764568/94‑1 (MQ=60)
                                                              CAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATC                                  >  SRR4302543.6150550/1‑94 (MQ=60)
                                                              CAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATC                                  >  SRR4302543.4531069/1‑94 (MQ=60)
                                                              CAAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATC                                  >  SRR4302543.5371329/1‑94 (MQ=60)
                                                               AAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCA                                 <  SRR4302543.5145859/94‑1 (MQ=60)
                                                               AAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCA                                 <  SRR4302543.4634475/94‑1 (MQ=60)
                                                               AAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCA                                 <  SRR4302543.4256672/94‑1 (MQ=60)
                                                               AAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCA                                 >  SRR4302543.4932161/1‑94 (MQ=60)
                                                               AAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCA                                 >  SRR4302543.4121651/1‑94 (MQ=60)
                                                               AAAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCA                                 >  SRR4302543.4121650/1‑94 (MQ=60)
                                                                AAGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAG                                <  SRR4302543.7241612/94‑1 (MQ=60)
                                                                 AGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGG                               <  SRR4302543.5781098/94‑1 (MQ=60)
                                                                 AGTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGG                               <  SRR4302543.4988096/94‑1 (MQ=60)
                                                                  GTACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGC                              <  SRR4302543.434990/94‑1 (MQ=60)
                                                                    ACGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAG                            >  SRR4302543.6388731/1‑94 (MQ=60)
                                                                     CGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGT                           >  SRR4302543.4161505/1‑94 (MQ=60)
                                                                     CGCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGT                           <  SRR4302543.2766582/94‑1 (MQ=60)
                                                                      cgGTTTTCCATGCCGGATGCGGCGCAAACCCCTTACCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTT                          <  SRR4302543.5667955/92‑1 (MQ=60)
                                                                      GCTTTTTCCATGCCGGATGCGCCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTT                          <  SRR4302543.2910302/94‑1 (MQ=60)
                                                                      GCGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTT                          >  SRR4302543.2014595/1‑94 (MQ=60)
                                                                       CGTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTT                         >  SRR4302543.7506014/1‑94 (MQ=60)
                                                                        GTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTT                        <  SRR4302543.933186/94‑1 (MQ=60)
                                                                        GTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTT                        >  SRR4302543.4720597/1‑94 (MQ=60)
                                                                        GTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTT                        >  SRR4302543.7400653/1‑94 (MQ=60)
                                                                        GTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTT                        >  SRR4302543.1022113/1‑94 (MQ=60)
                                                                        GTTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGAGCATCAGGCAGTTTT                        >  SRR4302543.3476208/1‑94 (MQ=60)
                                                                         TTTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTG                       <  SRR4302543.2750901/94‑1 (MQ=60)
                                                                          TTTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGC                      >  SRR4302543.91689/1‑94 (MQ=60)
                                                                           TTCCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCA                     <  SRR4302543.5611733/94‑1 (MQ=60)
                                                                             CCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATT                   >  SRR4302543.5436065/1‑94 (MQ=60)
                                                                             CCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATT                   <  SRR4302543.2799904/94‑1 (MQ=60)
                                                                             CCATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATT                   >  SRR4302543.4590716/1‑94 (MQ=60)
                                                                               ATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTG                 >  SRR4302543.6391179/1‑94 (MQ=60)
                                                                               ATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTG                 >  SRR4302543.4516412/1‑94 (MQ=60)
                                                                               ATGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTG                 <  SRR4302543.522819/94‑1 (MQ=60)
                                                                                TGCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGT                <  SRR4302543.7953537/94‑1 (MQ=60)
                                                                                 GCCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTC               <  SRR4302543.7982513/94‑1 (MQ=60)
                                                                                  CCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCA              <  SRR4302543.7985147/94‑1 (MQ=60)
                                                                                  CCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCA              >  SRR4302543.7303756/1‑94 (MQ=60)
                                                                                  CCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCA              >  SRR4302543.2132851/1‑94 (MQ=60)
                                                                                  CCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCA              <  SRR4302543.4247602/94‑1 (MQ=60)
                                                                                  CCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCA              <  SRR4302543.247212/94‑1 (MQ=60)
                                                                                  CCGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCA              >  SRR4302543.2075986/1‑94 (MQ=60)
                                                                                   CGGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCAT             >  SRR4302543.1494471/1‑94 (MQ=60)
                                                                                    GGATGCGGCGCAAACCCCTTTTCCCGCCTCCTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATC            <  SRR4302543.6074824/94‑1 (MQ=60)
                                                                                    GGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATC            <  SRR4302543.2693165/94‑1 (MQ=60)
                                                                                    GGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATC            <  SRR4302543.3978312/94‑1 (MQ=60)
                                                                                    GGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATC            >  SRR4302543.5208507/1‑94 (MQ=60)
                                                                                    GGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATC            >  SRR4302543.6318790/1‑94 (MQ=60)
                                                                                    GGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATC            >  SRR4302543.6544303/1‑94 (MQ=60)
                                                                                    GGATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATC            <  SRR4302543.6960384/94‑1 (MQ=60)
                                                                                     GATGCGGCGCAAACCCCTTATCCCGCCTTCTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCG           <  SRR4302543.7930342/94‑1 (MQ=60)
                                                                                     GATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCG           <  SRR4302543.170718/94‑1 (MQ=60)
                                                                                      ATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGC          >  SRR4302543.6648771/1‑94 (MQ=60)
                                                                                      ATGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGC          >  SRR4302543.6648770/1‑94 (MQ=60)
                                                                                       TGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCC         <  SRR4302543.6245520/94‑1 (MQ=60)
                                                                                       TGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCC         <  SRR4302543.2240374/94‑1 (MQ=60)
                                                                                       TGCGGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCC         >  SRR4302543.3535359/1‑94 (MQ=60)
                                                                                          GGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGT      >  SRR4302543.343633/1‑94 (MQ=60)
                                                                                          GGCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGT      >  SRR4302543.7661521/1‑94 (MQ=60)
                                                                                           GCGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTA     >  SRR4302543.4422540/1‑94 (MQ=60)
                                                                                            CGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTAT    <  SRR4302543.4196880/94‑1 (MQ=60)
                                                                                            CGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTAT    <  SRR4302543.7813295/94‑1 (MQ=60)
                                                                                            CGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTAT    <  SRR4302543.5585352/94‑1 (MQ=60)
                                                                                            CGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTAT    <  SRR4302543.4196879/94‑1 (MQ=60)
                                                                                            CGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTAT    <  SRR4302543.4081067/94‑1 (MQ=60)
                                                                                            CGCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTAT    >  SRR4302543.7219435/1‑94 (MQ=60)
                                                                                            CGCAAACACCTTACCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTAT    <  SRR4302543.3079401/94‑1 (MQ=60)
                                                                                             GCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATG   <  SRR4302543.3888525/94‑1 (MQ=60)
                                                                                             GCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATG   >  SRR4302543.4713038/1‑94 (MQ=60)
                                                                                             GCAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATG   >  SRR4302543.7162331/1‑94 (MQ=60)
                                                                                              CAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGC  <  SRR4302543.375251/94‑1 (MQ=60)
                                                                                              CAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGC  >  SRR4302543.1165102/1‑94 (MQ=60)
                                                                                              |                                                                                             
CGCATCATAGTCTTCCCTCAAG‑AAAA‑ATCAGGCCAGCATTTGCTGGCCCCAGATTGATAACAAAGTACGCGTTTTCCATGCCGGATGCGGCGTAAACACCTTATCCCGCCTACTCGATCGTGCATCTTTCCAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCATTTGTCATCGCCCGTATGC  >  NC_000913/3099635‑3099820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑