Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,160,251	A→G	T145T (ACT→ACC)	sufI ←	suppressor of ftsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,160,251	0	A	G	99.5%	579.7 / NA	184	T145T (ACT→ACC)	sufI	suppressor of ftsI
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (79/104); minor base T (1/0); total (80/104)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 194 total aligned reads displayed.

GTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCG  >  NC_000913/3160161‑3160339

gTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGt                                                                                        >  1:27254/1‑93 (MQ=255)

  tGGGGATAGGCAGCGTCTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTAt                                                                                      <  1:900192/93‑1 (MQ=255)

    gggATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTg                                                                                    <  1:2489841/93‑1 (MQ=255)

    gggATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTg                                                                                    <  2:2635352/93‑1 (MQ=255)

      gATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGc                                                                                  >  1:1180424/1‑93 (MQ=255)

      gATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGc                                                                                  >  2:1848156/1‑93 (MQ=255)

       aTAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTAAGCCGTGCGGTTGGGGGTATTGGCg                                                                                 >  2:780033/1‑93 (MQ=255)

           gCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGAt                                                                             <  2:3551503/93‑1 (MQ=255)

            cAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATa                                                                            <  1:284038/93‑1 (MQ=255)

            cAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATa                                                                            >  2:2877335/1‑93 (MQ=255)

             aGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATAc                                                                           <  1:3404889/93‑1 (MQ=255)

               cGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACca                                                                         <  2:1374014/93‑1 (MQ=255)

               cGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACca                                                                         <  1:1720141/93‑1 (MQ=255)

                gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcac                                                                        >  1:1448799/1‑93 (MQ=255)

                 aCTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcaca                                                                       >  1:3297168/1‑93 (MQ=255)

                      cTGATTTCATCTGCCACCAGCCACCTTCCGGCAAGGCCGTTATAGACGTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATTCCACAGATTa                                                                  <  2:1923577/93‑1 (MQ=255)

                      cTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTa                                                                  <  1:3555720/93‑1 (MQ=255)

                       tGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAg                                                                 >  2:4631164/1‑93 (MQ=255)

                       tGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAg                                                                 >  2:1735034/1‑93 (MQ=255)

                         aCTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCt                                                               <  1:547252/93‑1 (MQ=255)

                         aCTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCt                                                               >  1:641392/1‑93 (MQ=255)

                           ttCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGc                                                             >  1:1061963/1‑93 (MQ=255)

                           ttCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGc                                                             >  1:194781/1‑93 (MQ=255)

                             cATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGt                                                           <  2:2931658/93‑1 (MQ=255)

                               tCTTCCACCAGCCACATTCCTGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTc                                                         <  2:1412580/93‑1 (MQ=255)

                               tCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTc                                                         <  1:1391015/93‑1 (MQ=255)

                               tCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTc                                                         <  1:3980972/93‑1 (MQ=255)

                                cTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCt                                                        <  2:1435046/93‑1 (MQ=255)

                                cTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCt                                                        <  1:1645943/93‑1 (MQ=255)

                                 ttCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTg                                                       <  1:2219504/93‑1 (MQ=255)

                                 ttCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTg                                                       <  1:4457767/93‑1 (MQ=255)

                                 ttCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTg                                                       >  1:1843281/1‑93 (MQ=255)

                                 ttCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTg                                                       >  2:4654570/1‑93 (MQ=255)

                                    caccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCTGCGTGGTATGGGGTATTGGCGTGATATCACAGAGTATCTGCGTTCTGGCg                                                    >  2:4685758/1‑93 (MQ=255)

                                      ccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGaa                                                  <  1:410137/93‑1 (MQ=255)

                                       caGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAt                                                 >  2:3464546/1‑93 (MQ=255)

                                       caGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAt                                                 <  2:2854442/93‑1 (MQ=255)

                                        aGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATg                                                <  1:2675844/93‑1 (MQ=255)

                                         gCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATgg                                               <  1:152171/93‑1 (MQ=255)

                                          ccACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATggg                                              <  2:1465718/93‑1 (MQ=255)

                                          ccACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATggg                                              <  1:1976435/93‑1 (MQ=255)

                                           cacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGc                                             <  1:1128811/93‑1 (MQ=255)

                                           cacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGc                                             >  2:1457794/1‑93 (MQ=255)

                                           cacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACAACAGAGTAGCTGCGTTCTGGCGAATGGGc                                             >  1:156069/1‑93 (MQ=255)

                                            acaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCa                                            <  2:161230/93‑1 (MQ=255)

                                            acaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCa                                            >  1:1314262/1‑93 (MQ=255)

                                            acaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCa                                            >  1:2055615/1‑93 (MQ=255)

                                            acaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGGTGGGGGCATTGGCGTGAGATGACAGAGGAGCTGCGCTCTAGCGAATgggaa                                            >  2:3631841/1‑91 (MQ=255)

                                             caTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAg                                           >  2:4107281/1‑93 (MQ=255)

                                             caTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAg                                           <  1:2992353/93‑1 (MQ=255)

                                             caTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAg                                           <  2:1283859/93‑1 (MQ=255)

                                              aTTCCGGAAAGGCCGTTATAGACCTGCTGAGAAGTGCGGTTGGGGGTATTGGCGGGATAGTACAGTGTAGCTGCGTTCTGGCGACTGGGCAGt                                          >  2:3025783/1‑93 (MQ=255)

                                               ttCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTa                                         <  1:1008573/93‑1 (MQ=255)

                                                   ggCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                     <  2:1028747/93‑1 (MQ=255)

                                                   ggCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                     >  1:1173365/1‑93 (MQ=255)

                                                    gCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGt                                    <  1:2585262/93‑1 (MQ=255)

                                                    gCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGt                                    <  2:1199965/93‑1 (MQ=255)

                                                       aGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGcc                                 <  2:3392096/93‑1 (MQ=255)

                                                       aGGCCGTTATAGACCTGCTAAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGcc                                 >  1:3085576/1‑93 (MQ=255)

                                                        ggCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGccc                                <  2:1845922/93‑1 (MQ=255)

                                                          ccGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAg                              >  2:2057027/1‑93 (MQ=255)

                                                           cGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGt                             <  2:3332648/93‑1 (MQ=255)

                                                           cGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGt                             >  2:893334/1‑93 (MQ=255)

                                                           cGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGt                             <  2:671841/93‑1 (MQ=255)

                                                           cGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGt                             >  2:1104037/1‑93 (MQ=255)

                                                            gTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                            <  1:1635941/93‑1 (MQ=255)

                                                            gTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                            >  1:680862/1‑93 (MQ=255)

                                                             ttATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                           <  1:276385/93‑1 (MQ=255)

                                                             ttATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                           <  1:4650361/93‑1 (MQ=255)

                                                             ttATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                           <  2:3070949/93‑1 (MQ=255)

                                                              tataGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAg                          >  2:1672989/1‑93 (MQ=255)

                                                              tataGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAg                          >  1:1258083/1‑93 (MQ=255)

                                                              tataGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAg                          >  1:3775640/1‑93 (MQ=255)

                                                               ataGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGc                         >  1:1965789/1‑93 (MQ=255)

                                                               ataGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGc                         >  2:3184968/1‑93 (MQ=255)

                                                                taGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCg                        <  2:1489264/93‑1 (MQ=255)

                                                                  gACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCggt                      >  2:123184/1‑91 (MQ=255)

                                                                  gACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGtt                      <  1:2201947/93‑1 (MQ=255)

                                                                  gACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGtt                      >  1:2420818/1‑93 (MQ=255)

                                                                  gACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGtt                      >  1:587135/1‑93 (MQ=255)

                                                                   aCCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGttt                     >  2:2962064/1‑93 (MQ=255)

                                                                    ccTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTg                    <  1:3241942/93‑1 (MQ=255)

                                                                     ctgctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTgg                   >  1:40352/1‑93 (MQ=255)

                                                                      tgctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGc                  <  2:4324427/93‑1 (MQ=255)

                                                                          gAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAca              <  1:4365500/93‑1 (MQ=255)

                                                                          gAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAca              >  1:3914019/1‑93 (MQ=255)

                                                                           aGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcat             <  2:3967118/93‑1 (MQ=255)

                                                                            gCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatc            >  1:2569137/1‑93 (MQ=255)

                                                                              cGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatcat          <  1:793444/93‑1 (MQ=255)

                                                                              cGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatcat          <  2:3047797/93‑1 (MQ=255)

                                                                               gTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc         <  2:2436918/93‑1 (MQ=255)

                                                                               gTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc         <  2:3838641/93‑1 (MQ=255)

                                                                               gTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc         >  1:2382535/1‑93 (MQ=255)

                                                                                   ggTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTg     >  2:3511783/1‑93 (MQ=255)

                                                                                   ggTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTCCCCAGTCAGCGTTTGGCGACATCATCCGTg     <  1:718239/93‑1 (MQ=255)

                                                                                   gcttGGTAGTAGTGGCGTGATACCACAGAGTAGCTGCTTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTg     <  2:3122396/91‑1 (MQ=255)

                                                                                    gTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGc    <  2:3780805/93‑1 (MQ=255)

                                                                                     ttGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGcc   >  2:4272983/1‑93 (MQ=255)

                                                                                     ttGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGcc   >  2:2086179/1‑93 (MQ=255)

                                                                                      tGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCg  >  1:4241335/1‑93 (MQ=255)

GTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCG  >  NC_000913/3160161‑3160339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCG  >  NC_000913/3160160‑3160344

GGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGT                                                                                             >  SRR4302535.1464807/1‑94 (MQ=60)

GGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGT                                                                                             >  SRR4302535.142675/1‑94 (MQ=60)

GGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGT                                                                                             >  SRR4302535.27469/1‑94 (MQ=60)

  TTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTAT                                                                                           <  SRR4302535.3648287/94‑1 (MQ=60)

   TGGGGATAGGCAGCGTCTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATT                                                                                          <  SRR4302535.906566/94‑1 (MQ=60)

   TGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATT                                                                                          >  SRR4302535.2773112/1‑94 (MQ=60)

    GGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTG                                                                                         <  SRR4302535.2825066/94‑1 (MQ=60)

    GGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTG                                                                                         >  SRR4302535.1289219/1‑94 (MQ=60)

     GGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGG                                                                                        <  SRR4302535.2507799/94‑1 (MQ=60)

     GGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGG                                                                                        <  SRR4302535.4484458/94‑1 (MQ=60)

      GGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGC                                                                                       >  SRR4302535.1188853/1‑94 (MQ=60)

          AGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGA                                                                                   >  SRR4302535.3220195/1‑94 (MQ=60)

          AGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGA                                                                                   >  SRR4302535.1185815/1‑94 (MQ=60)

            GCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATA                                                                                 <  SRR4302535.4104995/94‑1 (MQ=60)

            GCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATA                                                                                 <  SRR4302535.1591515/94‑1 (MQ=60)

             CAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATAC                                                                                <  SRR4302535.286020/94‑1 (MQ=60)

              AGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACC                                                                               <  SRR4302535.3428583/94‑1 (MQ=60)

                CGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCAC                                                                             >  SRR4302535.1459156/1‑94 (MQ=60)

                CGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCAC                                                                             <  SRR4302535.1732582/94‑1 (MQ=60)

                 GACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACA                                                                            >  SRR4302535.3320091/1‑94 (MQ=60)

                   CTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGA                                                                          <  SRR4302535.2730984/94‑1 (MQ=60)

                     TGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGT                                                                        <  SRR4302535.1398627/94‑1 (MQ=60)

                       CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACCGCGTAG                                                                      >  SRR4302535.3299144/1‑94 (MQ=60)

                       CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAG                                                                      <  SRR4302535.3580382/94‑1 (MQ=60)

                         GACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCT                                                                    >  SRR4302535.645871/1‑94 (MQ=60)

                          ACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTG                                                                   <  SRR4302535.551074/94‑1 (MQ=60)

                           CTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGC                                                                  >  SRR4302535.196121/1‑94 (MQ=60)

                           CTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGC                                                                  >  SRR4302535.1069524/1‑94 (MQ=60)

                            tctATCTTCCACCAGCCACATGCCGGCAATGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCG                                                                 <  SRR4302535.470917/91‑1 (MQ=60)

                                TCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCT                                                             <  SRR4302535.4008168/94‑1 (MQ=60)

                                TCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCT                                                             <  SRR4302535.1400944/94‑1 (MQ=60)

                                 CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTG                                                            >  SRR4302535.1856574/1‑94 (MQ=60)

                                 CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTG                                                            <  SRR4302535.1657836/94‑1 (MQ=60)

                                 CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTG                                                            <  SRR4302535.2520953/94‑1 (MQ=60)

                                  TTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGG                                                           <  SRR4302535.3449132/94‑1 (MQ=60)

                                  TTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGG                                                           <  SRR4302535.4488339/94‑1 (MQ=60)

                                  TTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGG                                                           <  SRR4302535.2235294/94‑1 (MQ=60)

                                   TCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGC                                                          >  SRR4302535.1561870/1‑94 (MQ=60)

                                   TCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGC                                                          >  SRR4302535.678866/1‑94 (MQ=60)

                                   TCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGC                                                          <  SRR4302535.2442745/94‑1 (MQ=60)

                                       CCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAT                                                      <  SRR4302535.413019/94‑1 (MQ=60)

                                        CAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATG                                                     <  SRR4302535.2280334/94‑1 (MQ=60)

                                         AGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGG                                                    <  SRR4302535.2694989/94‑1 (MQ=60)

                                         AGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGG                                                    >  SRR4302535.2220580/1‑94 (MQ=60)

                                          GCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGG                                                   >  SRR4302535.1971032/1‑94 (MQ=60)

                                          GCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGG                                                   <  SRR4302535.153236/94‑1 (MQ=60)

                                           CCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGC                                                  <  SRR4302535.1990669/94‑1 (MQ=60)

                                           CCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACAACAGAGTAGCTGCGTTCTGGCGAATGGGC                                                  >  SRR4302535.157174/1‑94 (MQ=60)

                                            CACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCA                                                 >  SRR4302535.2070424/1‑94 (MQ=60)

                                            CACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCA                                                 <  SRR4302535.1136872/94‑1 (MQ=60)

                                            CACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCA                                                 >  SRR4302535.1323655/1‑94 (MQ=60)

                                              CATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGT                                               <  SRR4302535.3013333/94‑1 (MQ=60)

                                                TTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTAC                                             <  SRR4302535.1015739/94‑1 (MQ=60)

                                                TTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTAC                                             <  SRR4302535.3920873/94‑1 (MQ=60)

                                                   CGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGG                                          >  SRR4302535.1181742/1‑94 (MQ=60)

                                                     GCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTG                                        <  SRR4302535.2603827/94‑1 (MQ=60)

                                                       AAGGCCGTTATAGACCTGCTAAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCC                                      >  SRR4302535.3107069/1‑94 (MQ=60)

                                                        AGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCC                                     <  SRR4302535.696799/94‑1 (MQ=60)

                                                         GGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCA                                    <  SRR4302535.2122415/94‑1 (MQ=60)

                                                            CGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTC                                 >  SRR4302535.685628/1‑94 (MQ=60)

                                                             GTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCA                                <  SRR4302535.1647762/94‑1 (MQ=60)

                                                             GTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCA                                <  SRR4302535.1890504/94‑1 (MQ=60)

                                                              TTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAG                               <  SRR4302535.278314/94‑1 (MQ=60)

                                                              TTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAG                               >  SRR4302535.3801671/1‑94 (MQ=60)

                                                              TTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAG                               >  SRR4302535.1267083/1‑94 (MQ=60)

                                                              TTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAG                               <  SRR4302535.4681853/94‑1 (MQ=60)

                                                               TATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGC                              >  SRR4302535.1979958/1‑94 (MQ=60)

                                                                ATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCG                             >  SRR4302535.583206/1‑94 (MQ=60)

                                                                ATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCG                             >  SRR4302535.2016542/1‑94 (MQ=60)

                                                                ATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCG                             >  SRR4302535.2643226/1‑94 (MQ=60)

                                                                  AGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTT                           >  SRR4302535.2438249/1‑94 (MQ=60)

                                                                  AGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTT                           >  SRR4302535.591248/1‑94 (MQ=60)

                                                                  AGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTT                           <  SRR4302535.242388/94‑1 (MQ=60)

                                                                  AGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTT                           >  SRR4302535.2595088/1‑94 (MQ=60)

                                                                   GACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTT                          <  SRR4302535.743776/94‑1 (MQ=60)

                                                                   GACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTT                          <  SRR4302535.2217640/94‑1 (MQ=60)

                                                                     CCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGG                        <  SRR4302535.3264459/94‑1 (MQ=60)

                                                                     CCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGG                        <  SRR4302535.1136756/94‑1 (MQ=60)

                                                                     CCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGG                        <  SRR4302535.4668997/94‑1 (MQ=60)

                                                                     CCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGG                        >  SRR4302535.40659/1‑94 (MQ=60)

                                                                      CTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGC                       >  SRR4302535.1263504/1‑94 (MQ=60)

                                                                      CTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGAGCAGTACGGGTGCCCAGTCAGCGCTTGGC                       >  SRR4302535.300914/1‑94 (MQ=60)

                                                                       TGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCG                      <  SRR4302535.633206/94‑1 (MQ=60)

                                                                          TGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACA                   >  SRR4302535.3940807/1‑94 (MQ=60)

                                                                           GAGCCGTGCGGTTGGGGGTATTGGCGTGATGCCACAGAGTAGCTGCGTTCTGGCGAGTGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACAT                  <  SRR4302535.1708351/94‑1 (MQ=60)

                                                                           GAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACAT                  <  SRR4302535.4395372/94‑1 (MQ=60)

                                                                            AGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATC                 >  SRR4302535.2587616/1‑94 (MQ=60)

                                                                             GCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCA                <  SRR4302535.1648234/94‑1 (MQ=60)

                                                                             GCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCA                <  SRR4302535.1177662/94‑1 (MQ=60)

                                                                              CCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCAT               <  SRR4302535.4049808/94‑1 (MQ=60)

                                                                               CGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATC              >  SRR4302535.2399643/1‑94 (MQ=60)

                                                                               CGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATC              <  SRR4302535.799018/94‑1 (MQ=60)

                                                                                 TGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCG            >  SRR4302535.302725/1‑94 (MQ=60)

                                                                                   CGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTG          <  SRR4302535.950991/94‑1 (MQ=60)

                                                                                    GGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGC         >  SRR4302535.1845816/1‑94 (MQ=60)

                                                                                    GGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTCCCCAGTCAGCGTTTGGCGACATCATCCGTGC         <  SRR4302535.723285/94‑1 (MQ=60)

                                                                                     GTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCC        >  SRR4302535.4276883/1‑94 (MQ=60)

                                                                                      TTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCG       <  SRR4302535.3943867/94‑1 (MQ=60)

                                                                                      TTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCG       >  SRR4302535.4137781/1‑94 (MQ=60)

                                                                                      TTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCG       >  SRR4302535.4270364/1‑94 (MQ=60)

                                                                                        GGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGA     >  SRR4302535.4093629/1‑94 (MQ=60)

                                                                                         GGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGAC    <  SRR4302535.1938838/94‑1 (MQ=60)

                                                                                         GGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGAC    <  SRR4302535.2272037/94‑1 (MQ=60)

                                                                                          GGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACC   >  SRR4302535.650970/1‑94 (MQ=60)

                                                                                           GGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCG  >  SRR4302535.3361512/1‑94 (MQ=60)

GGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGACCG  >  NC_000913/3160160‑3160344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`