Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,160,251	A→G	T145T (ACT→ACC)	sufI ←	suppressor of ftsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,160,251	0	A	G	99.7%	911.4 / NA	289	T145T (ACT→ACC)	sufI	suppressor of ftsI
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (150/138); total (150/139)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

GTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGG  >  NC_000913/3160161‑3160340

 ttGGGGATAGTCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTa                                                                                        <  1:6991172/93‑1 (MQ=255)

 ttGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTa                                                                                        <  2:29286/93‑1 (MQ=255)

 ttGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTa                                                                                        <  2:4925252/93‑1 (MQ=255)

 ttGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGGTGGGGGTa                                                                                        >  1:1693575/1‑93 (MQ=255)

 gtGGGGATAGGCAGCGACTTGCGGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTa                                                                                        <  1:5375316/92‑1 (MQ=255)

  tGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTAt                                                                                       >  2:2840756/1‑93 (MQ=255)

      gATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGc                                                                                   <  1:5566278/93‑1 (MQ=255)

       aTAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCg                                                                                  >  1:2865594/1‑93 (MQ=255)

        tAGGCAGCGCCTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGt                                                                                 <  2:1963185/93‑1 (MQ=255)

        tAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGt                                                                                 >  1:4827869/1‑93 (MQ=255)

        tAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGt                                                                                 >  2:7198002/1‑93 (MQ=255)

        tAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGt                                                                                 <  2:7874489/93‑1 (MQ=255)

         aGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGGATTGGCGTg                                                                                >  2:6536056/1‑93 (MQ=255)

         aGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGGTGGGGGGATTGGCGTg                                                                                >  1:7636653/1‑93 (MQ=255)

          ggCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGa                                                                               <  2:5597765/93‑1 (MQ=255)

          ggCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGa                                                                               >  2:2635040/1‑93 (MQ=255)

            cAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATa                                                                             >  1:3095527/1‑93 (MQ=255)

            cAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATa                                                                             <  1:1393217/93‑1 (MQ=255)

                gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcac                                                                         <  2:5772661/93‑1 (MQ=255)

                gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcac                                                                         >  2:5816411/1‑93 (MQ=255)

                gACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcac                                                                         <  2:1749941/93‑1 (MQ=255)

                 cattGCGGACTTCCTCTACCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcaca                                                                        <  1:799326/91‑1 (MQ=255)

                 aCTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcaca                                                                        >  1:1802712/1‑93 (MQ=255)

                 aCTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACcaca                                                                        >  2:4603982/1‑93 (MQ=255)

                  cTTGCTGACTTCATCTTCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACag                                                                       <  1:2123506/93‑1 (MQ=255)

                  cTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACag                                                                       >  2:1452501/1‑93 (MQ=255)

                  cTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACag                                                                       >  1:105782/1‑93 (MQ=255)

                  cTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGGATTGGCGTGATACCACag                                                                       >  1:6576854/1‑93 (MQ=255)

                  cTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGCTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACag                                                                       >  2:6300825/1‑93 (MQ=255)

                   ttGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACaga                                                                      >  2:5920877/1‑93 (MQ=255)

                    tGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACagag                                                                     >  1:7767563/1‑93 (MQ=255)

                     gCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGCCCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGt                                                                    <  1:5219065/93‑1 (MQ=255)

                       tGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAg                                                                  >  2:3459649/1‑93 (MQ=255)

                         aCTTCATCTCCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCt                                                                <  2:4364631/93‑1 (MQ=255)

                               tCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTc                                                          >  1:665852/1‑93 (MQ=255)

                               tCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTc                                                          >  2:2191116/1‑93 (MQ=255)

                               tCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTc                                                          >  1:7216848/1‑93 (MQ=255)

                                cTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCt                                                         <  1:191484/93‑1 (MQ=255)

                                 ttCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTg                                                        >  2:766579/1‑93 (MQ=255)

                                 ttCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTg                                                        >  1:2463757/1‑93 (MQ=255)

                                  tCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTgg                                                       <  1:6778353/93‑1 (MQ=255)

                                      ccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGaa                                                   >  1:2769062/1‑93 (MQ=255)

                                      ccaGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGaa                                                   <  1:3317062/93‑1 (MQ=255)

                                       caGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCAAAGAGTAGCTGCGTTCTGGCGAAt                                                  <  1:4132683/93‑1 (MQ=255)

                                        aGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATg                                                 <  2:6603157/93‑1 (MQ=255)

                                         gCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATgg                                                <  2:1692741/93‑1 (MQ=255)

                                          ccACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATggg                                               >  1:5720889/1‑93 (MQ=255)

                                           cacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGc                                              <  1:2624075/93‑1 (MQ=255)

                                           cacaTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGc                                              >  2:965658/1‑93 (MQ=255)

                                             caTTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAg                                            >  2:7504715/1‑93 (MQ=255)

                                               ttCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTa                                          >  1:4248621/1‑93 (MQ=255)

                                               ttCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTa                                          <  1:7475123/93‑1 (MQ=255)

                                               ttCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTa                                          >  2:4167298/1‑93 (MQ=255)

                                               ttCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTa                                          <  1:6210967/93‑1 (MQ=255)

                                               ttCCGGCAAGGCCGTTATAGACCCGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTa                                          >  1:2591484/1‑93 (MQ=255)

                                               ttCCGGCAAGCCCGTTATAGCCCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTa                                          <  2:6226865/93‑1 (MQ=255)

                                                   ggCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                      >  2:6688790/1‑93 (MQ=255)

                                                   ggCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                      <  1:6417759/93‑1 (MQ=255)

                                                   ggCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                      >  2:5636832/1‑93 (MQ=255)

                                                   ggCAAGGCCGTGATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACggg                                      <  1:2009893/93‑1 (MQ=255)

                                                    gCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGt                                     <  2:68499/93‑1 (MQ=255)

                                                    gCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGt                                     <  2:7405227/93‑1 (MQ=255)

                                                    gCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGt                                     <  2:2304523/93‑1 (MQ=255)

                                                    gCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGt                                     <  2:3841109/93‑1 (MQ=255)

                                                      aaGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGc                                   >  2:780224/1‑93 (MQ=255)

                                                       aGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGcc                                  >  2:7058320/1‑93 (MQ=255)

                                                         gCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCa                                <  2:3685345/93‑1 (MQ=255)

                                                           cGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGt                              >  2:5022935/1‑93 (MQ=255)

                                                            gTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                             <  2:3122901/93‑1 (MQ=255)

                                                            gTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTc                             <  1:2065787/93‑1 (MQ=255)

                                                             ttATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                            >  1:4273025/1‑93 (MQ=255)

                                                             ttATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                            >  2:2350773/1‑93 (MQ=255)

                                                             ttATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                            >  1:3769641/1‑93 (MQ=255)

                                                             ttATAGACCTGCTGAGCAGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCa                            >  2:845544/1‑93 (MQ=255)

                                                              tataGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAg                           >  2:1224964/1‑93 (MQ=255)

                                                                taGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCg                         <  1:5882296/93‑1 (MQ=255)

                                                                  gACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGTCGAATGGGCAGTACGGGTGCCCAGTCAGCGtt                       >  1:4981621/1‑93 (MQ=255)

                                                                  gACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGtt                       <  2:3402110/93‑1 (MQ=255)

                                                                   aCCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGttt                      <  1:95684/93‑1 (MQ=255)

                                                                   aCCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGttt                      <  2:3309034/93‑1 (MQ=255)

                                                                     ctgctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCAAATGGGCAGTACGGGTGCCCAGTCAGCGTTTgg                    <  2:2746910/93‑1 (MQ=255)

                                                                      tgctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGc                   <  2:3165178/93‑1 (MQ=255)

                                                                      tgctgAGCCGTGCGGTTGGGGGGATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGc                   <  2:4881824/93‑1 (MQ=255)

                                                                        ctgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGa                 <  1:4515298/93‑1 (MQ=255)

                                                                         tgAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAc                >  1:1606653/1‑93 (MQ=255)

                                                                          gAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAca               <  1:4625022/93‑1 (MQ=255)

                                                                            gCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatc             <  2:5239587/93‑1 (MQ=255)

                                                                            gCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatc             >  2:5133066/1‑93 (MQ=255)

                                                                            gCCGTACGGTTGGGGGTATTGGCGTGATACCACGGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAcatc             <  2:6657139/93‑1 (MQ=255)

                                                                               gTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACatcatc          >  1:3557101/1‑93 (MQ=255)

                                                                               gTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGCGCCCAGTCAGCGTTTGGCGACatcatc          >  2:2546176/1‑93 (MQ=255)

                                                                                tGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATcc         <  1:7072569/93‑1 (MQ=255)

                                                                                tGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATcc         <  2:2102397/93‑1 (MQ=255)

                                                                                  cGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGt       >  2:4057695/1‑93 (MQ=255)

                                                                                  cGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGt       <  1:7401278/93‑1 (MQ=255)

                                                                                  cGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGt       <  2:6979119/93‑1 (MQ=255)

                                                                                   ggTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTg      <  2:6439117/93‑1 (MQ=255)

                                                                                    gTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGc     <  1:237069/93‑1 (MQ=255)

                                                                                     ttGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGcc    <  1:3940109/93‑1 (MQ=255)

                                                                                      tGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCg   >  1:5622775/1‑93 (MQ=255)

GTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGG  >  NC_000913/3160161‑3160340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGA  >  NC_000913/3160159‑3160341

TGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGG                                                                                           >  SRR4302543.6410288/1‑94 (MQ=60)

 GGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGT                                                                                          >  SRR4302543.5627485/1‑94 (MQ=60)

 GGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGT                                                                                          <  SRR4302543.6250861/94‑1 (MQ=60)

  GTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGGTGGGGGTA                                                                                         >  SRR4302543.1708766/1‑94 (MQ=60)

   TTGGGGATAGTCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTAT                                                                                        <  SRR4302543.7056971/94‑1 (MQ=60)

   GTGGGGATAGGCAGCGACTTGCGGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTAT                                                                                        <  SRR4302543.5424009/94‑1 (MQ=60)

    TGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATT                                                                                       <  SRR4302543.2073956/94‑1 (MQ=60)

    TGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATT                                                                                       >  SRR4302543.7529495/1‑94 (MQ=60)

     gtggataaaCAGCGACTTGCTGTCTGCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGGCCTGCTGAGCCGTGCGGTTGGGGGTATTG                                                                                      <  SRR4302543.2797173/85‑1 (MQ=60)

     GGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTG                                                                                      >  SRR4302543.3241596/1‑94 (MQ=60)

     GGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTG                                                                                      <  SRR4302543.5941653/94‑1 (MQ=60)

        GATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCG                                                                                   <  SRR4302543.5616913/94‑1 (MQ=60)

        GATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCG                                                                                   >  SRR4302543.2891714/1‑94 (MQ=60)

         ATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGT                                                                                  >  SRR4302543.4871921/1‑94 (MQ=60)

         ATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGT                                                                                  >  SRR4302543.298092/1‑94 (MQ=60)

          TAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGGTGGGGGGATTGGCGTG                                                                                 >  SRR4302543.7709102/1‑94 (MQ=60)

           AGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGA                                                                                >  SRR4302543.4298617/1‑94 (MQ=60)

            GGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGAT                                                                               <  SRR4302543.2871012/94‑1 (MQ=60)

             GCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATA                                                                              >  SRR4302543.3123369/1‑94 (MQ=60)

              CAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATAC                                                                             <  SRR4302543.1405765/94‑1 (MQ=60)

               AGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACC                                                                            >  SRR4302543.653264/1‑94 (MQ=60)

                 CGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCAC                                                                          >  SRR4302543.4339827/1‑94 (MQ=60)

                 CGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCAC                                                                          <  SRR4302543.4873430/94‑1 (MQ=60)

                  GACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACA                                                                         >  SRR4302543.1818845/1‑94 (MQ=60)

                  GACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACA                                                                         >  SRR4302543.7285086/1‑94 (MQ=60)

                   cattgcgGACTTCCTCTACCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAG                                                                        <  SRR4302543.806357/87‑1 (MQ=60)

                   ACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAG                                                                        <  SRR4302543.5799844/94‑1 (MQ=60)

                   ACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAG                                                                        >  SRR4302543.106754/1‑94 (MQ=60)

                   ACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGGATTGGCGTGATACCACAG                                                                        >  SRR4302543.6638787/1‑94 (MQ=60)

                    CTTGCTGACTTCATCTTCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGA                                                                       <  SRR4302543.2142518/94‑1 (MQ=60)

                    CTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGA                                                                       <  SRR4302543.3700433/94‑1 (MQ=60)

                     TTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAG                                                                      <  SRR4302543.2488432/94‑1 (MQ=60)

                     TTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAG                                                                      >  SRR4302543.7840849/1‑94 (MQ=60)

                      TGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGT                                                                     >  SRR4302543.5626450/1‑94 (MQ=60)

                       GCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGCCCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTA                                                                    <  SRR4302543.5266071/94‑1 (MQ=60)

                        CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAG                                                                   >  SRR4302543.5796323/1‑94 (MQ=60)

                        CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAG                                                                   <  SRR4302543.7730834/94‑1 (MQ=60)

                        CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAG                                                                   <  SRR4302543.4183831/94‑1 (MQ=60)

                        CTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAG                                                                   <  SRR4302543.6642726/94‑1 (MQ=60)

                         TGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGC                                                                  >  SRR4302543.2014289/1‑94 (MQ=60)

                         TGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGGATTGGCGTGATACCACAGAGTAGC                                                                  >  SRR4302543.3888229/1‑94 (MQ=60)

                          GACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCT                                                                 <  SRR4302543.1681192/94‑1 (MQ=60)

                          GACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCT                                                                 <  SRR4302543.4545801/94‑1 (MQ=60)

                          GACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCT                                                                 >  SRR4302543.6527999/1‑94 (MQ=60)

                           ACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTG                                                                >  SRR4302543.947420/1‑94 (MQ=60)

                           ACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTG                                                                >  SRR4302543.1963948/1‑94 (MQ=60)

                           ACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTG                                                                >  SRR4302543.2572318/1‑94 (MQ=60)

                             TTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGTGGGTATTGGCGTGATACCACAGAGTAGCTGCG                                                              <  SRR4302543.5184264/94‑1 (MQ=60)

                             TTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCG                                                              >  SRR4302543.3430090/1‑94 (MQ=60)

                              TCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGT                                                             <  SRR4302543.5300392/94‑1 (MQ=60)

                               CATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTT                                                            >  SRR4302543.7648135/1‑94 (MQ=60)

                               CATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTT                                                            >  SRR4302543.7319010/1‑94 (MQ=60)

                                CTCTTCCCCCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTC                                                           <  SRR4302543.6943666/94‑1 (MQ=60)

                                ATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTC                                                           >  SRR4302543.671979/1‑94 (MQ=60)

                                ATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTC                                                           >  SRR4302543.7286797/1‑94 (MQ=60)

                                  CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTG                                                         <  SRR4302543.193102/94‑1 (MQ=60)

                                  CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTG                                                         >  SRR4302543.2485976/1‑94 (MQ=60)

                                  CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTG                                                         <  SRR4302543.7717487/94‑1 (MQ=60)

                                  CTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTG                                                         <  SRR4302543.5466490/94‑1 (MQ=60)

                                   TTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGG                                                        <  SRR4302543.4164321/94‑1 (MQ=60)

                                    TCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGC                                                       >  SRR4302543.3666394/1‑94 (MQ=60)

                                    TCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGC                                                       <  SRR4302543.6842373/94‑1 (MQ=60)

                                     CCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCG                                                      >  SRR4302543.5748114/1‑94 (MQ=60)

                                      CACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGA                                                     <  SRR4302543.5614446/94‑1 (MQ=60)

                                       ACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAA                                                    >  SRR4302543.2794261/1‑94 (MQ=60)

                                       ACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAA                                                    <  SRR4302543.5241108/94‑1 (MQ=60)

                                        CCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAT                                                   <  SRR4302543.3346995/94‑1 (MQ=60)

                                        CCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAT                                                   <  SRR4302543.2462100/94‑1 (MQ=60)

                                        CCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAT                                                   >  SRR4302543.393907/1‑94 (MQ=60)

                                        CCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAAT                                                   <  SRR4302543.1857988/94‑1 (MQ=60)

                                         CAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATG                                                  <  SRR4302543.1580411/94‑1 (MQ=60)

                                         CAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCAAAGAGTAGCTGCGTTCTGGCGAATG                                                  <  SRR4302543.4169968/94‑1 (MQ=60)

                                          CGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGG                                                 <  SRR4302543.2735112/94‑1 (MQ=60)

                                          AGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGG                                                 >  SRR4302543.2275879/1‑94 (MQ=60)

                                           GCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGG                                                >  SRR4302543.5773090/1‑94 (MQ=60)

                                           GCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGG                                                >  SRR4302543.6199217/1‑94 (MQ=60)

                                            CCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGC                                               >  SRR4302543.6738436/1‑94 (MQ=60)

                                             CACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCA                                              <  SRR4302543.2647832/94‑1 (MQ=60)

                                             CACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCA                                              >  SRR4302543.2638811/1‑94 (MQ=60)

                                             CACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCA                                              >  SRR4302543.3984905/1‑94 (MQ=60)

                                              CCATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAG                                             <  SRR4302543.1786277/94‑1 (MQ=60)

                                              ACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAG                                             <  SRR4302543.4683728/94‑1 (MQ=60)

                                               CATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGT                                            <  SRR4302543.7244632/94‑1 (MQ=60)

                                                ATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTA                                           >  SRR4302543.5022799/1‑94 (MQ=60)

                                                ATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTA                                           >  SRR4302543.4287179/1‑94 (MQ=60)

                                                ATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTA                                           >  SRR4302543.4301734/1‑94 (MQ=60)

                                                ATTCCGGCAAGGCCGTTATAGACCCGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTA                                           >  SRR4302543.2615032/1‑94 (MQ=60)

                                                 TTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTAC                                          <  SRR4302543.7546696/94‑1 (MQ=60)

                                                 TTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTAC                                          <  SRR4302543.7887098/94‑1 (MQ=60)

                                                 TTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTAC                                          <  SRR4302543.6269611/94‑1 (MQ=60)

                                                 TTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTAC                                          <  SRR4302543.2054059/94‑1 (MQ=60)

                                                     GGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGT                                      <  SRR4302543.6478212/94‑1 (MQ=60)

                                                     GGCAAGGCCGTGATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGT                                      <  SRR4302543.2027954/94‑1 (MQ=60)

                                                      GCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTG                                     <  SRR4302543.5699742/94‑1 (MQ=60)

                                                           GCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAG                                >  SRR4302543.2285000/1‑94 (MQ=60)

                                                           GCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAG                                <  SRR4302543.4602013/94‑1 (MQ=60)

                                                           GCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGGATTGGCGTGATcccccagaggagctgggtgtgggcgaaggggcaggccgggtgacagg                                >  SRR4302543.2284999/1‑47 (MQ=60)

                                                            CCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGT                               <  SRR4302543.730574/94‑1 (MQ=60)

                                                            CCGTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGT                               <  SRR4302543.7534813/94‑1 (MQ=60)

                                                              GTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCA                             >  SRR4302543.4311859/1‑94 (MQ=60)

                                                              GTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCA                             <  SRR4302543.2084258/94‑1 (MQ=60)

                                                              GTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCA                             >  SRR4302543.3802830/1‑94 (MQ=60)

                                                              GTTATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCA                             <  SRR4302543.2136410/94‑1 (MQ=60)

                                                               TTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAG                            <  SRR4302543.6804886/94‑1 (MQ=60)

                                                                TATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGC                           >  SRR4302543.758352/1‑94 (MQ=60)

                                                                TATAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGC                           >  SRR4302543.824105/1‑94 (MQ=60)

                                                                  TAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGT                         <  SRR4302543.5935906/94‑1 (MQ=60)

                                                                  TAGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGT                         <  SRR4302543.2712838/94‑1 (MQ=60)

                                                                   AGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGTCGAATGGGCAGTACGGGTGCCCAGTCAGCGTT                        >  SRR4302543.5026969/1‑94 (MQ=60)

                                                                   AGACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTT                        <  SRR4302543.4738049/94‑1 (MQ=60)

                                                                    GACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTT                       <  SRR4302543.7942289/94‑1 (MQ=60)

                                                                    GACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTT                       >  SRR4302543.2484513/1‑94 (MQ=60)

                                                                    GACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTT                       <  SRR4302543.6877435/94‑1 (MQ=60)

                                                                     GCCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTG                      <  SRR4302543.2638405/94‑1 (MQ=60)

                                                                     ACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTG                      <  SRR4302543.6334254/94‑1 (MQ=60)

                                                                     ACCTGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTG                      <  SRR4302543.96573/94‑1 (MQ=60)

                                                                        TGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGTGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCG                   <  SRR4302543.5418625/94‑1 (MQ=60)

                                                                        TGCTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCG                   >  SRR4302543.1467681/1‑94 (MQ=60)

                                                                          CTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAC                 <  SRR4302543.4556367/94‑1 (MQ=60)

                                                                          CTGAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGAC                 >  SRR4302543.1620983/1‑94 (MQ=60)

                                                                            GAGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACAT               <  SRR4302543.4667041/94‑1 (MQ=60)

                                                                             AGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATC              >  SRR4302543.6647119/1‑94 (MQ=60)

                                                                             AGCCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATC              <  SRR4302543.2087959/94‑1 (MQ=60)

                                                                               CCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCGGCGTTTGGCGACATCAT            >  SRR4302543.1750587/1‑94 (MQ=60)

                                                                               CCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCAT            <  SRR4302543.457142/94‑1 (MQ=60)

                                                                               CCGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCAT            <  SRR4302543.1654860/94‑1 (MQ=60)

                                                                                CGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATC           >  SRR4302543.3588618/1‑94 (MQ=60)

                                                                                CGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATC           >  SRR4302543.1310172/1‑94 (MQ=60)

                                                                                CGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATC           >  SRR4302543.3245776/1‑94 (MQ=60)

                                                                                CGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATC           >  SRR4302543.3420668/1‑94 (MQ=60)

                                                                                CGTGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATC           >  SRR4302543.4205301/1‑94 (MQ=60)

                                                                                CGTGCGGTTGGGGGAATTGGCGTGATACCACAGAGCAGCTCCGTTCTGCCGAATGGGCAGTACGGGGGCCCGGCCAGCGTTTGGCaacatattc           >  SRR4302543.6797828/1‑85 (MQ=48)

                                                                                  TGCGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCG         <  SRR4302543.7141239/94‑1 (MQ=60)

                                                                                   GCGGTTGGGGGTATTGGCGTGATAACACAGAGTAGCTGCGTTCTGACGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCAACATCATCaat        <  SRR4302543.7103112/94‑4 (MQ=60)

                                                                                    CGGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTG       <  SRR4302543.7472327/94‑1 (MQ=60)

                                                                                     GGTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGC      >  SRR4302543.3072703/1‑94 (MQ=60)

                                                                                      GTTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCC     <  SRR4302543.239120/94‑1 (MQ=60)

                                                                                       TTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCG    >  SRR4302543.5674016/1‑94 (MQ=60)

                                                                                       TTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCG    <  SRR4302543.3974947/94‑1 (MQ=60)

                                                                                       TTGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCG    <  SRR4302543.1890645/94‑1 (MQ=60)

                                                                                        TGGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGG   <  SRR4302543.7128069/94‑1 (MQ=60)

                                                                                         GGGGGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGA  >  SRR4302543.3103685/1‑94 (MQ=60)

                                                                                         GGGGGTATTGGCGTGATACCACAGAGTAGCTGCGATCTTGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGA  >  SRR4302543.583118/1‑94 (MQ=60)

TGGTTGGGGATAGGCAGCGACTTGCTGACTTCATCTTCCACCAGCCACATTCCGGCAAGGCCGTTATAGACCTGCTGAGCCGTGCGGTTGGGAGTATTGGCGTGATACCACAGAGTAGCTGCGTTCTGGCGAATGGGCAGTACGGGTGCCCAGTCAGCGTTTGGCGACATCATCCGTGCCGGA  >  NC_000913/3160159‑3160341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`