Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,164,677 T→C K353R (AAG→AGG)  b3020 ← putative transport periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,164,6770TC100.0% 595.9 / NA 184K353R (AAG→AGG) b3020putative transport periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (101/83);  total (101/83)

Only 100 of 193 total aligned reads displayed.

AGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTT  >  NC_000913/3164588‑3164766
                                                                                         |                                                                                         
aGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCcttt                                                                                        <  1:4537031/93‑1 (MQ=255)
 gAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTg                                                                                       <  1:3912704/93‑1 (MQ=255)
 gAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTg                                                                                       <  1:2702078/93‑1 (MQ=255)
   aCAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCa                                                                                     >  2:333662/1‑93 (MQ=255)
   aCAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCa                                                                                     <  1:989510/93‑1 (MQ=255)
    cAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAg                                                                                    <  1:329210/93‑1 (MQ=255)
    cAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAg                                                                                    <  2:2604740/93‑1 (MQ=255)
    cAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAg                                                                                    <  1:2163282/93‑1 (MQ=255)
    cAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCCGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCCTTGGCCTCTGCAg                                                                                    >  1:1235808/1‑93 (MQ=255)
    cAGCTCAAAGCGTAGCGGATGAGAGGCGGCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAg                                                                                    <  1:3757587/93‑1 (MQ=255)
      gCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGtt                                                                                  >  2:2684398/1‑93 (MQ=255)
      gCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGtt                                                                                  >  2:655016/1‑93 (MQ=255)
       cTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTc                                                                                 <  1:1482165/93‑1 (MQ=255)
        tCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCa                                                                                <  1:4287633/93‑1 (MQ=255)
        tCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCa                                                                                <  1:3400407/93‑1 (MQ=255)
        tCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCAATCATTGGCCTTTGCAGTTCa                                                                                >  2:220538/1‑93 (MQ=255)
         cAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAATGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAt                                                                               <  2:3782169/93‑1 (MQ=255)
         cAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAt                                                                               <  2:3981660/93‑1 (MQ=255)
         cAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAt                                                                               <  2:2885420/93‑1 (MQ=255)
           aaGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATcc                                                                             >  1:3531182/1‑92 (MQ=255)
             gCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGaa                                                                           <  2:3143676/93‑1 (MQ=255)
             gCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGaa                                                                           >  2:3467910/1‑93 (MQ=255)
             gCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGaa                                                                           <  1:2607140/93‑1 (MQ=255)
              ggTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAAc                                                                          <  2:1720104/92‑1 (MQ=255)
               gTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAAcg                                                                         >  2:3382041/1‑93 (MQ=255)
                           agGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaa                                                             <  2:2773857/93‑1 (MQ=255)
                            ggCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaaa                                                            <  1:3154657/93‑1 (MQ=255)
                            ggCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaaa                                                            <  1:952252/93‑1 (MQ=255)
                            ggCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaaa                                                            <  1:2283706/93‑1 (MQ=255)
                            ggCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTaaa                                                            <  2:4258161/93‑1 (MQ=255)
                             gCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAg                                                           <  1:3032724/93‑1 (MQ=255)
                             gCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAg                                                           <  1:506961/93‑1 (MQ=255)
                              cgtcgtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGc                                                          >  1:4077147/1‑93 (MQ=255)
                              cgtcgtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGc                                                          <  2:4104743/93‑1 (MQ=255)
                               gtcgtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGcc                                                         <  2:4324777/93‑1 (MQ=255)
                               gtcgtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGcc                                                         <  1:4663626/93‑1 (MQ=255)
                                  gtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCttt                                                      <  1:2953994/93‑1 (MQ=255)
                                  gtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCttt                                                      >  2:150447/1‑93 (MQ=255)
                                  gtATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCttt                                                      >  1:4454828/1‑93 (MQ=255)
                                   tATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCtttt                                                     >  1:2724175/1‑93 (MQ=255)
                                    aTCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTa                                                    >  1:3763149/1‑93 (MQ=255)
                                    aTCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTa                                                    <  1:4348420/93‑1 (MQ=255)
                                    aTCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGACTTTTa                                                    >  2:3231911/1‑93 (MQ=255)
                                     tCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAc                                                   >  1:1282988/1‑93 (MQ=255)
                                     tCCCGCCTGTTTCAGCAAGGCTTTTACCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAc                                                   >  2:230402/1‑93 (MQ=255)
                                      cccGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAcc                                                  >  2:3627209/1‑93 (MQ=255)
                                       ccGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACct                                                 >  2:3603972/1‑93 (MQ=255)
                                        cGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACctc                                                >  2:2886425/1‑93 (MQ=255)
                                         gCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACctct                                               <  1:1594622/93‑1 (MQ=255)
                                         cccTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACctct                                               <  2:2265006/92‑1 (MQ=255)
                                          ccTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTg                                              >  1:4495602/1‑93 (MQ=255)
                                          ccTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTg                                              >  1:90238/1‑93 (MQ=255)
                                           cTGTTTCAGCAAGGCTTGTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTgg                                             <  1:707533/93‑1 (MQ=255)
                                             gTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcg                                           >  2:87593/1‑93 (MQ=255)
                                             gTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcg                                           >  2:3430336/1‑93 (MQ=255)
                                             gTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcg                                           >  2:2830969/1‑93 (MQ=255)
                                              tttCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGGGCTAAAGACTTTTCCCTCTggcgg                                          >  1:3575375/1‑93 (MQ=255)
                                              tttCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcgg                                          <  2:868997/93‑1 (MQ=255)
                                              tttCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTggcgg                                          >  2:1809904/1‑93 (MQ=255)
                                                 cAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGt                                       >  2:360007/1‑93 (MQ=255)
                                                  aGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTc                                      >  2:3821392/1‑93 (MQ=255)
                                                   gCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCa                                     <  2:3290050/93‑1 (MQ=255)
                                                     aaGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGc                                   >  2:1853408/1‑93 (MQ=255)
                                                      aGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCg                                  >  1:3575439/1‑93 (MQ=255)
                                                      aGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCg                                  >  1:4369349/1‑93 (MQ=255)
                                                       ggCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCgg                                 >  1:2774743/1‑92 (MQ=255)
                                                           tttGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGtt                             >  1:1713370/1‑93 (MQ=255)
                                                                cATCGCGACGCGCTCACTCTTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCggg                        >  2:4572178/1‑93 (MQ=255)
                                                                cATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCggg                        <  1:3210661/93‑1 (MQ=255)
                                                                 aTCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGc                       >  1:2004530/1‑93 (MQ=255)
                                                                 aTCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGc                       >  2:1087449/1‑93 (MQ=255)
                                                                  tCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCg                      >  2:1330733/1‑93 (MQ=255)
                                                                  tCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCg                      <  2:1772803/93‑1 (MQ=255)
                                                                    gcgACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGtt                    >  1:2654450/1‑93 (MQ=255)
                                                                      gACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTACAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTtct                  >  2:2844114/1‑93 (MQ=255)
                                                                      gACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTtct                  <  2:1154382/93‑1 (MQ=255)
                                                                       aCGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTtctc                 >  1:1320461/1‑93 (MQ=255)
                                                                       aCGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTtctc                 <  2:4401988/93‑1 (MQ=255)
                                                                         gcgcTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCaa               >  2:2944724/1‑93 (MQ=255)
                                                                         gcgcTCACTCAGTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCaa               <  2:735656/93‑1 (MQ=255)
                                                                           gcTCACTCTTTGGCCTTTGCGGTTCCTCGCCCGTCGTCGCGCTACCGCCTTTTACCTCTGGCGGCGTCCGCGTGGTTGCGGGCGTTCTCAccc             <  1:4687077/93‑1 (MQ=255)
                                                                            ctcactcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAAccc            >  2:2466545/1‑93 (MQ=255)
                                                                             tcactcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAAcccc           >  1:142915/1‑93 (MQ=255)
                                                                              cactcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCa          >  1:2922553/1‑93 (MQ=255)
                                                                              cactcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCa          >  1:3620452/1‑93 (MQ=255)
                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         <  1:834270/93‑1 (MQ=255)
                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         <  2:4209514/93‑1 (MQ=255)
                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         >  1:1764442/1‑93 (MQ=255)
                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         >  1:180239/1‑93 (MQ=255)
                                                                               actcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAg         >  1:3452969/1‑93 (MQ=255)
                                                                                ctcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGt        <  2:4267524/93‑1 (MQ=255)
                                                                                ctcaTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGt        >  2:3505536/1‑93 (MQ=255)
                                                                                  caTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTAc      <  2:2079059/93‑1 (MQ=255)
                                                                                  caTTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTAc      <  2:804797/93‑1 (MQ=255)
                                                                                    ttGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCt    >  2:1429191/1‑93 (MQ=255)
                                                                                    ttGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCt    >  1:4147314/1‑93 (MQ=255)
                                                                                    ttGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCt    <  2:2393671/93‑1 (MQ=255)
                                                                                     tGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCtt   <  1:1994574/93‑1 (MQ=255)
                                                                                     tGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCtt   >  1:3106203/1‑93 (MQ=255)
                                                                                      ggCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCttt  <  1:4269625/93‑1 (MQ=255)
                                                                                         |                                                                                         
AGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTT  >  NC_000913/3164588‑3164766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTTTGCG  >  NC_000913/3164584‑3164770
                                                                                             |                                                                                             
TTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCC                                                                                               >  SRR4302535.2122103/1‑94 (MQ=60)
 TGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCT                                                                                              >  SRR4302535.659501/1‑94 (MQ=60)
 TGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCT                                                                                              <  SRR4302535.3852143/94‑1 (MQ=60)
    AGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTG                                                                                           <  SRR4302535.4568068/94‑1 (MQ=60)
     GAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGC                                                                                          <  SRR4302535.2721383/94‑1 (MQ=60)
     GAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGC                                                                                          >  SRR4302535.3120791/1‑94 (MQ=60)
     GAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGC                                                                                          <  SRR4302535.3939486/94‑1 (MQ=60)
      AACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCA                                                                                         <  SRR4302535.4560171/94‑1 (MQ=60)
      AACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCA                                                                                         >  SRR4302535.4283647/1‑94 (MQ=60)
       ACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAG                                                                                        <  SRR4302535.996536/94‑1 (MQ=60)
       ACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCCGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCCTTGGCCTCTGCAG                                                                                        >  SRR4302535.1244637/1‑94 (MQ=60)
        CAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGT                                                                                       <  SRR4302535.3783572/94‑1 (MQ=60)
        CAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGT                                                                                       <  SRR4302535.331493/94‑1 (MQ=60)
        CAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGT                                                                                       <  SRR4302535.2178801/94‑1 (MQ=60)
        CAGCTCAAAGCGTAGCGGATGAGAGGCGGCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGT                                                                                       <  SRR4302535.3783571/94‑1 (MQ=60)
          GCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTC                                                                                     >  SRR4302535.1627597/1‑94 (MQ=60)
           CTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCA                                                                                    <  SRR4302535.1492764/94‑1 (MQ=60)
           CTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCA                                                                                    >  SRR4302535.303236/1‑94 (MQ=60)
           CTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCA                                                                                    >  SRR4302535.1300510/1‑94 (MQ=60)
            TCAAAGCGTAGCGGATGAGAGTCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAT                                                                                   <  SRR4302535.1405665/94‑1 (MQ=60)
            TCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAT                                                                                   <  SRR4302535.4316966/94‑1 (MQ=60)
            TCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAT                                                                                   <  SRR4302535.4405968/94‑1 (MQ=60)
            TCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCAT                                                                                   <  SRR4302535.3424075/94‑1 (MQ=60)
              AAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCC                                                                                 >  SRR4302535.3555697/1‑94 (MQ=60)
                 GCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAAC                                                                              <  SRR4302535.2625848/94‑1 (MQ=60)
                  CGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACG                                                                             >  SRR4302535.4485530/1‑94 (MQ=60)
                  CGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACG                                                                             >  SRR4302535.61226/1‑94 (MQ=60)
                   GTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGT                                                                            >  SRR4302535.4128237/1‑94 (MQ=60)
                     AGCGGAGGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCG                                                                          <  SRR4302535.953753/94‑1 (MQ=60)
                       GGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTC                                                                        <  SRR4302535.1744502/94‑1 (MQ=60)
                             AGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTA                                                                  >  SRR4302535.3435818/1‑94 (MQ=60)
                             AGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTA                                                                  <  SRR4302535.4305235/94‑1 (MQ=60)
                             AGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTA                                                                  >  SRR4302535.827121/1‑94 (MQ=60)
                               AGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAA                                                                <  SRR4302535.3981892/94‑1 (MQ=60)
                                GGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAG                                                               <  SRR4302535.2742057/94‑1 (MQ=60)
                                GGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAG                                                               <  SRR4302535.2300024/94‑1 (MQ=60)
                                GGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAG                                                               <  SRR4302535.3176571/94‑1 (MQ=60)
                                GGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAG                                                               <  SRR4302535.959004/94‑1 (MQ=60)
                                 GCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGC                                                              <  SRR4302535.3053953/94‑1 (MQ=60)
                                 GCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGC                                                              >  SRR4302535.4105045/1‑94 (MQ=60)
                                 GCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGC                                                              <  SRR4302535.99463/94‑1 (MQ=60)
                                 GCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGC                                                              <  SRR4302535.510506/94‑1 (MQ=60)
                                  CGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCC                                                             >  SRR4302535.1810250/1‑94 (MQ=60)
                                   GTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCT                                                            <  SRR4302535.4695160/94‑1 (MQ=60)
                                   GTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCT                                                            >  SRR4302535.2471260/1‑94 (MQ=60)
                                     CGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTT                                                          >  SRR4302535.4485378/1‑94 (MQ=60)
                                     CGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTT                                                          >  SRR4302535.506879/1‑94 (MQ=60)
                                      GTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTT                                                         <  SRR4302535.2974745/94‑1 (MQ=60)
                                      GTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTT                                                         >  SRR4302535.2743630/1‑94 (MQ=60)
                                       TATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTA                                                        >  SRR4302535.3789147/1‑94 (MQ=60)
                                        ATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAC                                                       <  SRR4302535.4378170/94‑1 (MQ=60)
                                        ATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAC                                                       >  SRR4302535.1292137/1‑94 (MQ=60)
                                        ATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAC                                                       <  SRR4302535.3373850/94‑1 (MQ=60)
                                        ATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAC                                                       >  SRR4302535.3133653/1‑94 (MQ=60)
                                        ATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTAC                                                       >  SRR4302535.3206265/1‑94 (MQ=60)
                                             GCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTG                                                  >  SRR4302535.90875/1‑94 (MQ=60)
                                             GCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTG                                                  >  SRR4302535.4526462/1‑94 (MQ=60)
                                             GCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTG                                                  <  SRR4302535.1606126/94‑1 (MQ=60)
                                               CTGTTTCAGCAAGGCTTGTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGC                                                <  SRR4302535.712498/94‑1 (MQ=60)
                                                TGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGGG                                               >  SRR4302535.3662061/1‑94 (MQ=60)
                                                 GTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGGGCTAAAGACTTTTCCCTCTGGCGG                                              >  SRR4302535.3600162/1‑94 (MQ=60)
                                                      AGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGTCTTTTACCTCTGGCGGCGTCG                                         >  SRR4302535.1876487/1‑94 (MQ=60)
                                                       GCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAG                                        >  SRR4302535.4397537/1‑94 (MQ=60)
                                                        CAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGC                                       <  SRR4302535.297221/94‑1 (MQ=60)
                                                         AAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCG                                      >  SRR4302535.4399251/1‑94 (MQ=60)
                                                         AAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCG                                      >  SRR4302535.3600226/1‑94 (MQ=60)
                                                         AAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCG                                      <  SRR4302535.2788265/94‑1 (MQ=60)
                                                          AGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGT                                     >  SRR4302535.2936065/1‑94 (MQ=60)
                                                          AGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGG                                     >  SRR4302535.2794498/1‑94 (MQ=60)
                                                            GCTTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGG                                   <  SRR4302535.3479795/94‑1 (MQ=60)
                                                              TTTTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTT                                 >  SRR4302535.1725757/1‑94 (MQ=60)
                                                                TTGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGC                               <  SRR4302535.3559765/94‑1 (MQ=60)
                                                                 TGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCG                              >  SRR4302535.1474515/1‑94 (MQ=60)
                                                                 TGCCATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCG                              <  SRR4302535.904367/94‑1 (MQ=60)
                                                                    CATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGC                           >  SRR4302535.2018973/1‑94 (MQ=60)
                                                                    CATCGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGC                           <  SRR4302535.3232899/94‑1 (MQ=60)
                                                                       CGCGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTT                        >  SRR4302535.2673467/1‑94 (MQ=60)
                                                                         CGACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCT                      >  SRR4302535.1494612/1‑94 (MQ=60)
                                                                          GACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTC                     >  SRR4302535.1329893/1‑94 (MQ=60)
                                                                           ACGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCA                    >  SRR4302535.3258427/1‑94 (MQ=60)
                                                                            CGCGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAA                   >  SRR4302535.4352996/1‑94 (MQ=60)
                                                                              CGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACC                 >  SRR4302535.2017667/1‑94 (MQ=60)
                                                                              CGCTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACC                 >  SRR4302535.2037964/1‑94 (MQ=60)
                                                                               GCTCACTCTTTGGCCTTTGCGGTTCCTCGCCCGTCGTCGCGCTACCGCCTTTTACCTCTGGCGGCGTCCGCGTGGTTGCGGGCGTTCTCACCCC                <  SRR4302535.4718885/94‑1 (MQ=44)
                                                                                CTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCC               >  SRR4302535.2297248/1‑94 (MQ=60)
                                                                                CTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCC               >  SRR4302535.143911/1‑94 (MQ=60)
                                                                                CTCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCC               <  SRR4302535.3633923/94‑1 (MQ=60)
                                                                                 TCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCA              >  SRR4302535.3865651/1‑94 (MQ=60)
                                                                                 TCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCA              >  SRR4302535.2943164/1‑94 (MQ=60)
                                                                                 TCACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCA              >  SRR4302535.3645550/1‑94 (MQ=60)
                                                                                  CACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAG             >  SRR4302535.1777229/1‑94 (MQ=60)
                                                                                  CACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAG             >  SRR4302535.3476971/1‑94 (MQ=60)
                                                                                  CACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAG             >  SRR4302535.181493/1‑94 (MQ=60)
                                                                                   ACTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGT            <  SRR4302535.840118/94‑1 (MQ=60)
                                                                                    CTCATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTA           >  SRR4302535.2664847/1‑94 (MQ=60)
                                                                                      CATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACC         >  SRR4302535.4712358/1‑94 (MQ=60)
                                                                                       ATTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCT        >  SRR4302535.4175713/1‑94 (MQ=60)
                                                                                        TTGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTT       >  SRR4302535.3127828/1‑94 (MQ=60)
                                                                                         TGGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTT      <  SRR4302535.2008938/94‑1 (MQ=60)
                                                                                          GGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTTT     <  SRR4302535.4298857/94‑1 (MQ=60)
                                                                                          GGCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTTT     >  SRR4302535.951051/1‑94 (MQ=60)
                                                                                           GCCTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTTTG    >  SRR4302535.2886725/1‑94 (MQ=60)
                                                                                             CTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTTTGCG  >  SRR4302535.2851990/1‑94 (MQ=60)
                                                                                             CTTTGAAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTTTGCT  >  SRR4302535.2551371/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTGTAGAACAGCTCAAAGCGTAGCGGATGAGAGGCGTCGTATCCCGCCTGTTTCAGCAAGGCTTTTGCCATCGCGACGCGCTCACTCATTGGCTTTTGCAGTTCATCGAACGTCGTCGCGCTAAAGCCTTTTACCTCTGGCGGCGTCAGCGTGGTTGCGGGCGTTCTCAACCCCAGTACCTTTTGCG  >  NC_000913/3164584‑3164770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑