Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,186,089 T→C I225T (ATC→ACC)  yqiG → putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,186,0890TC99.5% 685.6 / ‑5.5 218I225T (ATC→ACC) yqiGputative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (106/111);  total (107/111)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 224 total aligned reads displayed.

TACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGA  >  NC_000913/3186000‑3186178
                                                                                         |                                                                                         
tACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAAcccc                                                                                        >  1:1733490/1‑93 (MQ=255)
 aCTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCa                                                                                       <  1:2146367/93‑1 (MQ=255)
 aCTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCa                                                                                       <  2:2702969/93‑1 (MQ=255)
 aCTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCa                                                                                       <  1:2868519/93‑1 (MQ=255)
  cTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAg                                                                                      >  1:324495/1‑93 (MQ=255)
  cTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAg                                                                                      <  2:1552061/93‑1 (MQ=255)
  cTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAg                                                                                      <  2:1099795/93‑1 (MQ=255)
  cTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAg                                                                                      <  2:535982/93‑1 (MQ=255)
  cTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAg                                                                                      <  1:690057/93‑1 (MQ=255)
  cTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGACGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAg                                                                                      >  1:2350171/1‑93 (MQ=255)
    gATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCa                                                                                    >  1:4556221/1‑93 (MQ=255)
      ttACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCaat                                                                                  >  1:3515224/1‑93 (MQ=255)
      ttACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCaat                                                                                  <  1:2767020/93‑1 (MQ=255)
      ttACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCaat                                                                                  >  1:1905202/1‑93 (MQ=255)
         ccAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCaatgaa                                                                               <  2:1275522/93‑1 (MQ=255)
         ccAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCaatgaa                                                                               >  1:1002407/1‑93 (MQ=255)
         ccAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCaatgaa                                                                               <  2:2401638/93‑1 (MQ=255)
         ccAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCaatgaa                                                                               <  1:2023259/93‑1 (MQ=255)
          cAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCaatgaat                                                                              <  2:2173898/93‑1 (MQ=255)
            gTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGc                                                                            <  2:4245677/93‑1 (MQ=255)
                gATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCaaaa                                                                        <  2:1947383/93‑1 (MQ=255)
                gATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCaaaa                                                                        >  2:2010838/1‑93 (MQ=255)
                gATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCaaaa                                                                        <  2:4297140/93‑1 (MQ=255)
                 aTCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAGCCCCAGCAATGAATGCAAaac                                                                       <  2:2869187/93‑1 (MQ=255)
                 aTCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAaac                                                                       >  2:412886/1‑93 (MQ=255)
                 aTCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAaac                                                                       <  2:2244859/93‑1 (MQ=255)
                    aGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAaactaa                                                                    >  2:2134653/1‑93 (MQ=255)
                     gTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAaactaac                                                                   <  2:1710530/93‑1 (MQ=255)
                       cATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAactaacta                                                                 >  1:2977087/1‑93 (MQ=255)
                       cATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAactaacta                                                                 >  1:856063/1‑93 (MQ=255)
                       cATTTAACAACGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAactaacta                                                                 <  2:37832/93‑1 (MQ=255)
                         tttAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACtata                                                               >  1:1837550/1‑93 (MQ=255)
                         tttAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACtata                                                               <  1:4375956/93‑1 (MQ=255)
                         tttAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACtata                                                               <  2:437590/93‑1 (MQ=255)
                         tttAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACtata                                                               <  1:2594704/93‑1 (MQ=255)
                         tttAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACtata                                                               >  1:3605898/1‑93 (MQ=255)
                          ttAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAg                                                              >  2:2521989/1‑93 (MQ=255)
                          ttAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAg                                                              <  1:3759777/93‑1 (MQ=255)
                          ttAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAg                                                              >  1:3078004/1‑93 (MQ=255)
                          ttAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAg                                                              <  2:4504931/93‑1 (MQ=255)
                             aCAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTc                                                           <  1:784817/93‑1 (MQ=255)
                             aCAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTc                                                           >  2:3123080/1‑93 (MQ=255)
                               aaTGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCaa                                                         <  2:2800938/93‑1 (MQ=255)
                                 tGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATa                                                       <  2:449991/93‑1 (MQ=255)
                                  ggCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc                                                      <  1:4024260/93‑1 (MQ=255)
                                  ggCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc                                                      <  1:4407839/93‑1 (MQ=255)
                                  ggCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc                                                      <  2:748548/93‑1 (MQ=255)
                                  ggCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc                                                      <  1:3461990/93‑1 (MQ=255)
                                    cAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGa                                                    <  2:858913/93‑1 (MQ=255)
                                     aaaaGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGAt                                                   >  1:560990/1‑93 (MQ=255)
                                     aaaaGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGAt                                                   <  1:3259599/93‑1 (MQ=255)
                                     aaaaGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGAt                                                   <  2:3503869/93‑1 (MQ=255)
                                     aaaaGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGAt                                                   <  1:4218569/93‑1 (MQ=255)
                                      aaaGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATa                                                  >  2:1179148/1‑93 (MQ=255)
                                      aaaGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATa                                                  <  2:1380545/93‑1 (MQ=255)
                                        aGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTAAATACGATACt                                                <  1:4069167/93‑1 (MQ=255)
                                         gCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTg                                               >  2:358182/1‑93 (MQ=255)
                                           cAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGaa                                             >  1:394535/1‑93 (MQ=255)
                                           cAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGaa                                             <  2:2224841/93‑1 (MQ=255)
                                            aGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAAt                                            >  1:1317118/1‑93 (MQ=255)
                                            aGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAAt                                            >  1:3671742/1‑93 (MQ=255)
                                            aGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAAt                                            <  1:340877/93‑1 (MQ=255)
                                             ggCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATc                                           >  2:4542805/1‑93 (MQ=255)
                                             ggCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATc                                           <  1:2359930/93‑1 (MQ=255)
                                                 aCCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCctct                                       <  1:364540/93‑1 (MQ=255)
                                                  ccAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTa                                      >  2:4467044/1‑93 (MQ=255)
                                                   cAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTAt                                     <  1:1053370/93‑1 (MQ=255)
                                                    aaCCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTAtt                                    <  1:1545020/93‑1 (MQ=255)
                                                     aCCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTAttt                                   <  2:3485478/93‑1 (MQ=255)
                                                      ccTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTAtttt                                  <  2:3088841/93‑1 (MQ=255)
                                                        tGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCg                                >  1:124301/1‑93 (MQ=255)
                                                        tGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCg                                >  2:3264213/1‑93 (MQ=255)
                                                        tGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATACGTCAATACGATACTGAATCCTCTATTTTCg                                >  2:4209394/1‑93 (MQ=255)
                                                          gATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGAc                              <  2:2891856/93‑1 (MQ=255)
                                                          gATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGAc                              <  2:3194970/93‑1 (MQ=255)
                                                            ttttCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGActc                            >  2:1879324/1‑93 (MQ=255)
                                                              ttCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCtt                          >  1:3720414/1‑93 (MQ=255)
                                                               tCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCttt                         <  1:2656962/93‑1 (MQ=255)
                                                                ccGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTAAAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTc                        <  2:714954/93‑1 (MQ=255)
                                                                    gTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCAtt                    >  1:3167876/1‑93 (MQ=255)
                                                                    gTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCAtt                    <  1:1744543/93‑1 (MQ=255)
                                                                    gTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCAtt                    >  1:2538493/1‑93 (MQ=255)
                                                                     tatttattTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCAttt                   >  2:2825824/1‑93 (MQ=255)
                                                                      ttttatttGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCAtttt                  <  2:3368755/92‑1 (MQ=255)
                                                                      atttatttGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCAtttt                  >  1:1117777/1‑93 (MQ=255)
                                                                       tttatttGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCAttttt                 <  1:1797587/93‑1 (MQ=255)
                                                                       tttatttGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCAttttt                 >  2:3863835/1‑93 (MQ=255)
                                                                       tttatttGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCAttttt                 <  2:3445174/93‑1 (MQ=255)
                                                                        ttatttGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTc                >  1:1191648/1‑93 (MQ=255)
                                                                        ttatttGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTc                >  1:2894985/1‑93 (MQ=255)
                                                                         tatttGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCt               <  1:1700358/93‑1 (MQ=255)
                                                                           tttGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTgg             >  1:1590025/1‑93 (MQ=255)
                                                                              gTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCAt          >  1:3435032/1‑93 (MQ=255)
                                                                               tttCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCAtt         >  1:750643/1‑93 (MQ=255)
                                                                                 tCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTc       <  1:1749048/93‑1 (MQ=255)
                                                                                   gCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGt     >  1:990468/1‑93 (MQ=255)
                                                                                    cccAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGtt    <  2:4051070/93‑1 (MQ=255)
                                                                                     ccAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTg   <  2:2305335/93‑1 (MQ=255)
                                                                                      cAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGa  <  2:1464613/93‑1 (MQ=255)
                                                                                      cAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGa  <  1:4264849/93‑1 (MQ=255)
                                                                                         |                                                                                         
TACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGA  >  NC_000913/3186000‑3186178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAG  >  NC_000913/3185998‑3186182
                                                                                           |                                                                                             
CGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACAAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCC                                                                                             <  SRR4302535.1934851/94‑1 (MQ=60)
 GTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCC                                                                                            >  SRR4302535.1746018/1‑94 (MQ=60)
 GTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCC                                                                                            >  SRR4302535.3600657/1‑94 (MQ=60)
   ACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAG                                                                                          <  SRR4302535.2161808/94‑1 (MQ=60)
   ACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAG                                                                                          <  SRR4302535.2888836/94‑1 (MQ=60)
   ACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAG                                                                                          >  SRR4302535.326739/1‑94 (MQ=60)
   ACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGACGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAG                                                                                          >  SRR4302535.2367018/1‑94 (MQ=60)
    CTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGC                                                                                         <  SRR4302535.694893/94‑1 (MQ=60)
     TGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCA                                                                                        >  SRR4302535.4587336/1‑94 (MQ=60)
     TGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCA                                                                                        <  SRR4302535.4255923/94‑1 (MQ=60)
      GATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGCATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAA                                                                                       <  SRR4302535.2668820/94‑1 (MQ=60)
       ATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAAT                                                                                      >  SRR4302535.3539650/1‑94 (MQ=60)
       ATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAAT                                                                                      >  SRR4302535.1918914/1‑94 (MQ=60)
       ATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAAT                                                                                      >  SRR4302535.565433/1‑94 (MQ=60)
       ATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAAT                                                                                      >  SRR4302535.1029800/1‑94 (MQ=60)
        TTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATG                                                                                     <  SRR4302535.2786725/94‑1 (MQ=60)
          ACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAA                                                                                   >  SRR4302535.1009525/1‑94 (MQ=60)
           CCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAAT                                                                                  <  SRR4302535.1565868/94‑1 (MQ=60)
           CCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAAT                                                                                  <  SRR4302535.2037842/94‑1 (MQ=60)
                 CGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAA                                                                            >  SRR4302535.4116201/1‑94 (MQ=60)
                 CGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAA                                                                            >  SRR4302535.1120836/1‑94 (MQ=60)
                  GATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAAC                                                                           <  SRR4302535.3855496/94‑1 (MQ=60)
                  GATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAAC                                                                           <  SRR4302535.2633154/94‑1 (MQ=60)
                   ATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGCTTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACT                                                                          >  SRR4302535.3288752/1‑94 (MQ=60)
                   ATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACT                                                                          >  SRR4302535.758841/1‑94 (MQ=60)
                   ATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACT                                                                          >  SRR4302535.2371517/1‑94 (MQ=60)
                      AGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAAC                                                                       <  SRR4302535.440505/94‑1 (MQ=60)
                      AGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAAC                                                                       <  SRR4302535.182410/94‑1 (MQ=60)
                      AGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAAC                                                                       >  SRR4302535.1295477/1‑94 (MQ=60)
                        TCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTA                                                                     >  SRR4302535.2997973/1‑94 (MQ=60)
                        TCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTA                                                                     <  SRR4302535.73484/94‑1 (MQ=60)
                        TCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTA                                                                     >  SRR4302535.862097/1‑94 (MQ=60)
                         CATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTAT                                                                    >  SRR4302535.1760042/1‑94 (MQ=60)
                          ATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATA                                                                   >  SRR4302535.4413293/1‑94 (MQ=60)
                          ATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATA                                                                   >  SRR4302535.3630912/1‑94 (MQ=60)
                          ATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATA                                                                   >  SRR4302535.221391/1‑94 (MQ=60)
                          ATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATA                                                                   <  SRR4302535.1715472/94‑1 (MQ=60)
                          ATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATA                                                                   >  SRR4302535.1850807/1‑94 (MQ=60)
                           TTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAG                                                                  <  SRR4302535.2613345/94‑1 (MQ=60)
                           TTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAG                                                                  <  SRR4302535.4405897/94‑1 (MQ=60)
                           TTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAG                                                                  >  SRR4302535.3099473/1‑94 (MQ=60)
                           TTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAG                                                                  <  SRR4302535.1409255/94‑1 (MQ=60)
                            TTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGG                                                                 <  SRR4302535.3785764/94‑1 (MQ=60)
                            TTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGG                                                                 <  SRR4302535.3785765/94‑1 (MQ=60)
                            TTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGG                                                                 >  SRR4302535.2058272/1‑94 (MQ=60)
                              AACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCACAGCAATGAATGCAAAACTAACTATAGGTC                                                               >  SRR4302535.3434428/1‑94 (MQ=60)
                               ACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCA                                                              <  SRR4302535.2571165/94‑1 (MQ=60)
                               ACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCA                                                              <  SRR4302535.1831953/94‑1 (MQ=60)
                               ACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCA                                                              >  SRR4302535.3053448/1‑94 (MQ=60)
                               ACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCA                                                              <  SRR4302535.790323/94‑1 (MQ=60)
                                 AATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAAT                                                            >  SRR4302535.3606531/1‑94 (MQ=60)
                                 AATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAAT                                                            >  SRR4302535.3652379/1‑94 (MQ=60)
                                  ATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATA                                                           >  SRR4302535.4114096/1‑94 (MQ=60)
                                   TGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAC                                                          <  SRR4302535.1900767/94‑1 (MQ=60)
                                    GGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACG                                                         <  SRR4302535.3486059/94‑1 (MQ=60)
                                    GGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACG                                                         <  SRR4302535.4438003/94‑1 (MQ=60)
                                    GGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACG                                                         <  SRR4302535.4051795/94‑1 (MQ=60)
                                      CAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGAT                                                       >  SRR4302535.564913/1‑94 (MQ=60)
                                       AAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATA                                                      <  SRR4302535.3282254/94‑1 (MQ=60)
                                       AAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATA                                                      <  SRR4302535.4247436/94‑1 (MQ=60)
                                       AAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATA                                                      >  SRR4302535.36794/1‑94 (MQ=60)
                                        AAAGCCAGGCGACCAACCTGCATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATAC                                                     <  SRR4302535.4063903/94‑1 (MQ=60)
                                         AAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACT                                                    >  SRR4302535.4491226/1‑94 (MQ=60)
                                          AGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTAAATACGATACTG                                                   <  SRR4302535.4097015/94‑1 (MQ=60)
                                            CCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAA                                                 >  SRR4302535.397289/1‑94 (MQ=60)
                                            CCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAA                                                 >  SRR4302535.175579/1‑94 (MQ=60)
                                             CAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAAT                                                >  SRR4302535.3697170/1‑94 (MQ=60)
                                             CAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAAT                                                >  SRR4302535.1326528/1‑94 (MQ=60)
                                              AGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATC                                               <  SRR4302535.343241/94‑1 (MQ=60)
                                               GGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCC                                              <  SRR4302535.2376865/94‑1 (MQ=60)
                                                   ACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTA                                          <  SRR4302535.367049/94‑1 (MQ=60)
                                                     CAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATT                                        <  SRR4302535.1060860/94‑1 (MQ=60)
                                                      AACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTT                                       <  SRR4302535.1556146/94‑1 (MQ=60)
                                                      AACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTT                                       <  SRR4302535.3280445/94‑1 (MQ=60)
                                                      AACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTT                                       <  SRR4302535.880485/94‑1 (MQ=60)
                                                       ACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTT                                      >  SRR4302535.2743801/1‑94 (MQ=60)
                                                        CCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTC                                     <  SRR4302535.4602580/94‑1 (MQ=60)
                                                         CTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCG                                    >  SRR4302535.125173/1‑94 (MQ=60)
                                                         CTGGATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCG                                    <  SRR4302535.4045923/94‑1 (MQ=60)
                                                            GATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACT                                 <  SRR4302535.1994820/94‑1 (MQ=60)
                                                             ATTTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTC                                >  SRR4302535.762150/1‑94 (MQ=60)
                                                               TTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTT                              >  SRR4302535.3505449/1‑94 (MQ=60)
                                                               TTTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTT                              >  SRR4302535.3746190/1‑94 (MQ=60)
                                                                TTCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTT                             <  SRR4302535.3760013/94‑1 (MQ=60)
                                                                 TCCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTC                            <  SRR4302535.2675997/94‑1 (MQ=60)
                                                                  CCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCC                           >  SRR4302535.1146148/1‑94 (MQ=60)
                                                                  CCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCC                           >  SRR4302535.3355904/1‑94 (MQ=60)
                                                                  CCGCGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTCCGACTCTTTCC                           >  SRR4302535.2189718/1‑94 (MQ=60)
                                                                     CGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATT                        >  SRR4302535.3189822/1‑94 (MQ=60)
                                                                     CGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATT                        >  SRR4302535.2883000/1‑94 (MQ=60)
                                                                     CGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATT                        >  SRR4302535.173591/1‑94 (MQ=60)
                                                                     CGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATT                        >  SRR4302535.2556779/1‑94 (MQ=60)
                                                                     CGTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATT                        >  SRR4302535.3109905/1‑94 (MQ=60)
                                                                      TTATTTATTTGTTTCGCCCGACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTT                       <  SRR4302535.3221175/94‑1 (MQ=60)
                                                                      GTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTT                       >  SRR4302535.1210025/1‑94 (MQ=60)
                                                                      GTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTT                       >  SRR4302535.2937233/1‑94 (MQ=60)
                                                                      GTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTT                       >  SRR4302535.1667181/1‑94 (MQ=60)
                                                                      GTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTT                       <  SRR4302535.1757166/94‑1 (MQ=60)
                                                                      GTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTT                       <  SRR4302535.1789812/94‑1 (MQ=60)
                                                                      GTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTT                       >  SRR4302535.792183/1‑94 (MQ=60)
                                                                      GTATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTT                       >  SRR4302535.3707133/1‑94 (MQ=60)
                                                                       TATTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTT                      >  SRR4302535.1125750/1‑94 (MQ=60)
                                                                         TTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTC                    <  SRR4302535.1810581/94‑1 (MQ=60)
                                                                         TTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTC                    >  SRR4302535.2915460/1‑94 (MQ=60)
                                                                         TTTATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTC                    >  SRR4302535.1200181/1‑94 (MQ=60)
                                                                           TATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTG                  <  SRR4302535.2952567/94‑1 (MQ=60)
                                                                           TATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTG                  <  SRR4302535.1712643/94‑1 (MQ=60)
                                                                            ATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGG                 >  SRR4302535.1601500/1‑94 (MQ=60)
                                                                            ATTTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGG                 <  SRR4302535.3462079/94‑1 (MQ=60)
                                                                              TTGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCA               <  SRR4302535.2400025/94‑1 (MQ=60)
                                                                               TGTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCAT              >  SRR4302535.3458905/1‑94 (MQ=60)
                                                                                GTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATT             <  SRR4302535.2895780/94‑1 (MQ=60)
                                                                                GTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATT             >  SRR4302535.755934/1‑94 (MQ=60)
                                                                                GTTTCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATT             <  SRR4302535.4527854/94‑1 (MQ=60)
                                                                                   TCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCG          <  SRR4302535.1306466/94‑1 (MQ=60)
                                                                                   TCGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCG          <  SRR4302535.1761700/94‑1 (MQ=60)
                                                                                    CGCCCAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGT         >  SRR4302535.997501/1‑94 (MQ=60)
                                                                                        CAACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAA     <  SRR4302535.4294055/94‑1 (MQ=60)
                                                                                          ACCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAA   <  SRR4302535.3676901/94‑1 (MQ=60)
                                                                                           CCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAG  >  SRR4302535.1084675/1‑94 (MQ=60)
                                                                                           |                                                                                             
CGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCATTTTTCTGGCATTTCGTTGAAAAG  >  NC_000913/3185998‑3186182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑