Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,729	A→G	V15A (GTG→GCG)	bacA ←	BacA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,729	0	A	G	100.0%	466.6 / NA	150	V15A (GTG→GCG)	bacA	BacA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (56/94); total (56/94)

Only 100 of 154 total aligned reads displayed.

GTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACT  >  NC_000913/3201642‑3201818

gtgtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCAccc                                                                                      >  2:659871/1‑93 (MQ=255)

 tgtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCa                                                                                     <  1:502168/93‑1 (MQ=255)

  gtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCaa                                                                                    <  2:2694794/93‑1 (MQ=255)

    cGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAata                                                                                  >  1:3745311/1‑93 (MQ=255)

     gCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAatat                                                                                 <  2:2847428/93‑1 (MQ=255)

     gCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAatat                                                                                 <  2:3605840/93‑1 (MQ=255)

     ccccTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAatat                                                                                 <  1:2446361/92‑1 (MQ=255)

      cccTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAtt                                                                                <  1:1072122/93‑1 (MQ=255)

      cccTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAtt                                                                                >  1:1192910/1‑93 (MQ=255)

      cccTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAtt                                                                                <  2:1769102/93‑1 (MQ=255)

      cccTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAtt                                                                                <  1:687907/93‑1 (MQ=255)

       ccTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTg                                                                               >  1:4275539/1‑93 (MQ=255)

        cTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGc                                                                              >  1:1924450/1‑93 (MQ=255)

        cTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGc                                                                              <  2:1167968/93‑1 (MQ=255)

         tCAACCCCCAGCAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGcc                                                                             <  2:3701705/93‑1 (MQ=255)

           aaaCCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGc                                                                           <  1:3052498/93‑1 (MQ=255)

               cccAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATCTTGCCGCTATc                                                                       >  1:2782223/1‑93 (MQ=255)

               cccAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATc                                                                       <  1:324196/93‑1 (MQ=255)

                 caacaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcag                                                                     >  1:3590879/1‑93 (MQ=255)

                  aacaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagc                                                                    <  1:153668/93‑1 (MQ=255)

                   acaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagca                                                                   <  2:4481363/93‑1 (MQ=255)

                   acaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagca                                                                   >  1:1147228/1‑93 (MQ=255)

                   acaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagca                                                                   <  1:90129/93‑1 (MQ=255)

                   acaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagca                                                                   >  1:2088023/1‑93 (MQ=255)

                   acaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagca                                                                   >  1:3118735/1‑93 (MQ=255)

                    caaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagcag                                                                  <  2:4586388/93‑1 (MQ=255)

                     aaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCagcagc                                                                 <  2:2321009/93‑1 (MQ=255)

                       gTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCgg                                                               >  2:2386666/1‑92 (MQ=255)

                       gTGACCAACAATAATCATAAGGCCCGAGTTGAATACCGGCAGAAATTTTGCCACTCCTCCGACCTCACCCAGTATTGCCGCTATCAGCAGCGa                                                               <  2:3104819/93‑1 (MQ=255)

                        tGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAg                                                              >  2:2728655/1‑93 (MQ=255)

                           ccGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGc                                                           <  2:1660436/93‑1 (MQ=255)

                           ccGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGc                                                           >  2:593366/1‑93 (MQ=255)

                           ccGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGc                                                           <  1:2742336/93‑1 (MQ=255)

                            cGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCa                                                          >  1:4047356/1‑93 (MQ=255)

                              aCAAAAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCata                                                        <  2:2326076/93‑1 (MQ=255)

                                aataatCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATc                                                      >  2:3555544/1‑93 (MQ=255)

                                  taatCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGc                                                    <  1:1888353/93‑1 (MQ=255)

                                  taatCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGc                                                    >  1:235550/1‑93 (MQ=255)

                                  taatCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGc                                                    <  2:2175385/93‑1 (MQ=255)

                                  taatCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGc                                                    >  1:2320148/1‑93 (MQ=255)

                                  taatCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGc                                                    <  1:2926233/93‑1 (MQ=255)

                                    atCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtc                                                  >  1:3485806/1‑93 (MQ=255)

                                      cATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtcat                                                <  1:4214380/93‑1 (MQ=255)

                                       atatGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtcatc                                               >  1:3764360/1‑93 (MQ=255)

                                       atatGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtcatc                                               <  2:1059447/93‑1 (MQ=255)

                                        tatGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtcatca                                              >  2:4017465/1‑93 (MQ=255)

                                         atGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCaa                                             <  1:231839/93‑1 (MQ=255)

                                          tGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAAt                                            >  1:3237995/1‑93 (MQ=255)

                                           tgCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATa                                           <  1:446940/92‑1 (MQ=255)

                                           ggCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATa                                           <  2:3333312/93‑1 (MQ=255)

                                           ggCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATa                                           <  1:4396182/93‑1 (MQ=255)

                                            gCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATaa                                          <  1:2783694/93‑1 (MQ=255)

                                            gCCCGTGATGGATACCGGCAGAAATTCTGTCAACCCTTCGACCGCGCCCAATATTGCCGCTATCAGTATGGAGTTCGTATCGCTCATCAATaa                                          <  2:1285470/93‑1 (MQ=255)

                                             cccGTGCTGGATGCCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATaaa                                         <  1:3158848/93‑1 (MQ=255)

                                             cccGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATaaa                                         <  1:199513/93‑1 (MQ=255)

                                              ccGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAc                                        <  2:1529773/93‑1 (MQ=255)

                                              ccGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAc                                        <  2:4595497/93‑1 (MQ=255)

                                              ccGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAc                                        <  2:1440838/93‑1 (MQ=255)

                                               cGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAcc                                       <  2:2497875/93‑1 (MQ=255)

                                                 tGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAcccc                                     <  1:4617891/93‑1 (MQ=255)

                                                 tGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAcccc                                     <  2:3385375/93‑1 (MQ=255)

                                                 tGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAcccc                                     <  1:224680/93‑1 (MQ=255)

                                                  gCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCt                                    >  1:1047077/1‑93 (MQ=255)

                                                  gCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTACATATCGCTCATCAATAAACCCCt                                    <  2:690155/93‑1 (MQ=255)

                                                    tGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTaa                                  >  1:1366169/1‑93 (MQ=255)

                                                     ggATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTaaa                                 >  2:1631569/1‑93 (MQ=255)

                                                      gATACCGGCAGAAATTCTGTCAATCCTTCGACCGCGCCCAATATTGCCGCTATCAGCAGCGAGTCCATATCGCTCATCAATAAACCCCTAAat                                <  1:3178372/93‑1 (MQ=255)

                                                      gATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAat                                >  1:2713262/1‑93 (MQ=255)

                                                      gATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAat                                <  2:323099/93‑1 (MQ=255)

                                                         aCCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAattat                             >  1:1934031/1‑93 (MQ=255)

                                                         aCCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAattat                             <  2:366154/93‑1 (MQ=255)

                                                          ccGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAattatt                            <  1:3799023/93‑1 (MQ=255)

                                                            ggCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTaa                          >  2:4467819/1‑93 (MQ=255)

                                                              cAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTaaaa                        <  2:1809135/93‑1 (MQ=255)

                                                              cAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTaaaa                        <  1:3320312/93‑1 (MQ=255)

                                                               aGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAAt                       <  2:3314726/93‑1 (MQ=255)

                                                                gAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATg                      <  2:1324311/93‑1 (MQ=255)

                                                                 aaaTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGt                     <  1:3082641/93‑1 (MQ=255)

                                                                 aaaTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGt                     <  1:2538472/93‑1 (MQ=255)

                                                                  aaTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTa                    <  1:2905424/93‑1 (MQ=255)

                                                                   aTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAc                   <  2:4173672/93‑1 (MQ=255)

                                                                    ttCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAcc                  >  2:658982/1‑93 (MQ=255)

                                                                    ttCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAcc                  <  1:619880/93‑1 (MQ=255)

                                                                      cTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGc                <  2:3089473/93‑1 (MQ=255)

                                                                      cTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGc                >  1:3895085/1‑93 (MQ=255)

                                                                      cTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGc                <  2:3382786/93‑1 (MQ=255)

                                                                       tGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCt               >  1:3074680/1‑93 (MQ=255)

                                                                       tGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGAGAGTGCATATCGCTCATCAATAAATCCCTAAATTATTAAAATGTACCGCt               >  1:819375/1‑93 (MQ=255)

                                                                        gTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCtt              >  1:4486331/1‑93 (MQ=255)

                                                                         tCAATGCTTCGAACGCAACCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAACCCTAAAATATTACAATGTACCGCTTg             >  2:524350/1‑93 (MQ=255)

                                                                         tCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATCTCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTg             <  1:3276114/93‑1 (MQ=255)

                                                                            aTCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTcc          >  1:398555/1‑93 (MQ=255)

                                                                             tCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTACAATGTACCGCTTGTCCg         >  2:2083497/1‑93 (MQ=255)

                                                                               cTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGaa       <  2:4087477/93‑1 (MQ=255)

                                                                               cTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGaa       <  1:3549452/93‑1 (MQ=255)

                                                                                ttCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAac      <  1:601753/93‑1 (MQ=255)

                                                                                   gACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAactac   >  1:2618996/1‑93 (MQ=255)

                                                                                    aCCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAactact  <  1:4127003/93‑1 (MQ=255)

                                                                                    aCCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAactact  <  2:3248849/93‑1 (MQ=255)

                                                                                    aCCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAactact  <  1:1312293/93‑1 (MQ=255)

GTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACT  >  NC_000913/3201642‑3201818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCG  >  NC_000913/3201643‑3201821

TGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAA                                                                                       <  SRR4302535.505681/94‑1 (MQ=60)

 GTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAAT                                                                                      >  SRR4302535.4546166/1‑94 (MQ=60)

 GTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAAT                                                                                      <  SRR4302535.1461578/94‑1 (MQ=60)

  TCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATA                                                                                     >  SRR4302535.3771225/1‑94 (MQ=60)

   CGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAT                                                                                    >  SRR4302535.2250402/1‑94 (MQ=60)

    GCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATT                                                                                   >  SRR4302535.1201459/1‑94 (MQ=60)

    CCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATT                                                                                   <  SRR4302535.2463983/94‑1 (MQ=60)

     CCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTG                                                                                  <  SRR4302535.1079737/94‑1 (MQ=60)

     CCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTG                                                                                  <  SRR4302535.692726/94‑1 (MQ=60)

     CCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTG                                                                                  >  SRR4302535.4304805/1‑94 (MQ=60)

      CCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGC                                                                                 >  SRR4302535.1266303/1‑94 (MQ=60)

      CCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGC                                                                                 >  SRR4302535.1938320/1‑94 (MQ=60)

       CTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCC                                                                                >  SRR4302535.4501072/1‑94 (MQ=60)

       CTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCC                                                                                >  SRR4302535.2914592/1‑94 (MQ=60)

       CTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCC                                                                                >  SRR4302535.3620297/1‑94 (MQ=60)

        TCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCG                                                                               <  SRR4302535.3329129/94‑1 (MQ=60)

          AAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCT                                                                             <  SRR4302535.3073875/94‑1 (MQ=60)

           AACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTA                                                                            >  SRR4302535.4202239/1‑94 (MQ=60)

            ACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTAT                                                                           <  SRR4302535.3780127/94‑1 (MQ=60)

             CCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATCTTGCCGCTATC                                                                          >  SRR4302535.2802027/1‑94 (MQ=60)

              CCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCA                                                                         <  SRR4302535.326435/94‑1 (MQ=60)

               CCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAG                                                                        >  SRR4302535.3615784/1‑94 (MQ=60)

               CCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAG                                                                        >  SRR4302535.4038527/1‑94 (MQ=60)

                 CACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCA                                                                      <  SRR4302535.3044208/94‑1 (MQ=60)

                 AACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCA                                                                      <  SRR4302535.388197/94‑1 (MQ=60)

                 AACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCA                                                                      >  SRR4302535.3140448/1‑94 (MQ=60)

                 AACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCA                                                                      <  SRR4302535.154748/94‑1 (MQ=60)

                 AACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCA                                                                      >  SRR4302535.1155401/1‑94 (MQ=60)

                 AACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCA                                                                      >  SRR4302535.2103068/1‑94 (MQ=60)

                  ACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAG                                                                     <  SRR4302535.90764/94‑1 (MQ=60)

                   CAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGC                                                                    <  SRR4302535.1992267/94‑1 (MQ=60)

                     AGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGA                                                                  <  SRR4302535.267705/94‑1 (MQ=60)

                      ATGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAG                                                                 <  SRR4302535.1154791/94‑1 (MQ=60)

                          CCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCA                                                             <  SRR4302535.2761881/94‑1 (MQ=60)

                          CCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCA                                                             >  SRR4302535.4075061/1‑94 (MQ=60)

                             ACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATAT                                                          <  SRR4302535.3195441/94‑1 (MQ=60)

                             ACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATAT                                                          <  SRR4302535.4343987/94‑1 (MQ=60)

                               AATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCG                                                        <  SRR4302535.835925/94‑1 (MQ=60)

                                ATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTTAATCCTTCGACCGCACACAATATTGCCGCTATCAGCAGCGAGTGCATATCGC                                                       >  SRR4302535.2795697/1‑94 (MQ=60)

                                ATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGC                                                       >  SRR4302535.2336754/1‑94 (MQ=60)

                                ATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGC                                                       >  SRR4302535.237178/1‑94 (MQ=60)

                                 TAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCT                                                      <  SRR4302535.2946861/94‑1 (MQ=60)

                                 TAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCT                                                      <  SRR4302535.1901954/94‑1 (MQ=60)

                                  AATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTC                                                     >  SRR4302535.3510005/1‑94 (MQ=60)

                                   ATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCA                                                    >  SRR4302535.3092107/1‑94 (MQ=60)

                                     CATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATC                                                  <  SRR4302535.4243223/94‑1 (MQ=60)

                                     CATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATC                                                  >  SRR4302535.3790364/1‑94 (MQ=60)

                                        ATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAAT                                               >  SRR4302535.3260473/1‑94 (MQ=60)

                                        ATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAAT                                               <  SRR4302535.233438/94‑1 (MQ=60)

                                          TGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAA                                             <  SRR4302535.450069/94‑1 (MQ=60)

                                          GGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAA                                             <  SRR4302535.4426265/94‑1 (MQ=60)

                                          GGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAA                                             >  SRR4302535.2341340/1‑94 (MQ=60)

                                          GGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAA                                             <  SRR4302535.65619/94‑1 (MQ=60)

                                           GCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAA                                            <  SRR4302535.2331337/94‑1 (MQ=60)

                                           GCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAA                                            <  SRR4302535.2803502/94‑1 (MQ=60)

                                            CCCGTGCTGGATGCCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAC                                           <  SRR4302535.3180769/94‑1 (MQ=60)

                                            CCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAC                                           <  SRR4302535.200880/94‑1 (MQ=60)

                                             CCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACC                                          <  SRR4302535.3263390/94‑1 (MQ=60)

                                                TGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCT                                       <  SRR4302535.226228/94‑1 (MQ=60)

                                                TGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCT                                       <  SRR4302535.4649274/94‑1 (MQ=60)

                                                TGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCT                                       >  SRR4302535.1054502/1‑94 (MQ=60)

                                                  CTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAA                                     >  SRR4302535.1375937/1‑94 (MQ=60)

                                                    GGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAAT                                   >  SRR4302535.2732647/1‑94 (MQ=60)

                                                     GATACCGGCAGAAATTCTGTCAATCCTTCGACCGCGCCCAATATTGCCGCTATCAGCAGCGAGTCCATATCGCTCATCAATAAACCCCTAAATT                                  <  SRR4302535.3200354/94‑1 (MQ=60)

                                                     GATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATT                                  >  SRR4302535.3567360/1‑94 (MQ=60)

                                                       TACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTAT                                >  SRR4302535.1947960/1‑94 (MQ=60)

                                                         CCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTA                              <  SRR4302535.3825212/94‑1 (MQ=60)

                                                            GCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAA                           <  SRR4302535.1910056/94‑1 (MQ=60)

                                                             CAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAAT                          <  SRR4302535.3343414/94‑1 (MQ=60)

                                                                AAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTA                       <  SRR4302535.2556757/94‑1 (MQ=60)

                                                                AAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTA                       <  SRR4302535.3104124/94‑1 (MQ=60)

                                                                 AATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGATTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAC                      <  SRR4302535.2539375/94‑1 (MQ=60)

                                                                 AATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAC                      <  SRR4302535.2925961/94‑1 (MQ=60)

                                                                   TTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCG                    <  SRR4302535.624191/94‑1 (MQ=60)

                                                                    TCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGC                   >  SRR4302535.3921781/1‑94 (MQ=60)

                                                                     CTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCT                  >  SRR4302535.3096139/1‑94 (MQ=60)

                                                                     CTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGAGAGTGCATATCGCTCATCAATAAATCCCTAAATTATTAAAATGTACCGCT                  >  SRR4302535.825129/1‑94 (MQ=60)

                                                                      TGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTT                 >  SRR4302535.4517126/1‑94 (MQ=60)

                                                                        TCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATCTCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGT               <  SRR4302535.3298873/94‑1 (MQ=60)

                                                                        TCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGT               <  SRR4302535.3140220/94‑1 (MQ=60)

                                                                          AATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCC             >  SRR4302535.401347/1‑94 (MQ=60)

                                                                           ATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCG            >  SRR4302535.515727/1‑94 (MQ=60)

                                                                              CTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAAC         <  SRR4302535.3574087/94‑1 (MQ=60)

                                                                               TTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACT        <  SRR4302535.605961/94‑1 (MQ=60)

                                                                                 CGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTAC      >  SRR4302535.2637774/1‑94 (MQ=60)

                                                                                   ACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTG    <  SRR4302535.1321674/94‑1 (MQ=60)

                                                                                   ACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTG    <  SRR4302535.4155244/94‑1 (MQ=60)

                                                                                     CGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCG  <  SRR4302535.2838003/94‑1 (MQ=60)

TGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCG  >  NC_000913/3201643‑3201821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`