Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1696	279.3	15874187	97.8%	15524954	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,201,729	A→G	V15A (GTG→GCG)	bacA ←	BacA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,201,729	0	A	G	99.3%	895.3 / ‑3.6	287	V15A (GTG→GCG)	bacA	BacA
Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (127/158); total (129/158)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

Only 100 of 293 total aligned reads displayed.

GCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACT  >  NC_000913/3201639‑3201818

  cGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGTCAGAAATTCTGTCAATCCTTCGACCGCAcc                                                                                       >  2:6455130/1‑93 (MQ=255)

  cGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCAcc                                                                                       >  1:4867586/1‑93 (MQ=255)

   gtgtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCAccc                                                                                      >  2:5979777/1‑93 (MQ=255)

    tgtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCa                                                                                     >  1:3687555/1‑93 (MQ=255)

     gtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCaa                                                                                    <  1:1421138/93‑1 (MQ=255)

     gtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCaa                                                                                    >  1:7335922/1‑93 (MQ=255)

     gtCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCaa                                                                                    >  2:6084122/1‑93 (MQ=255)

       cGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAata                                                                                  >  2:2353581/1‑93 (MQ=255)

        gCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAatat                                                                                 <  1:7379474/93‑1 (MQ=255)

        gCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAatat                                                                                 >  1:5598693/1‑93 (MQ=255)

        gCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAatat                                                                                 <  1:5306392/93‑1 (MQ=255)

         cccTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAtt                                                                                >  2:230478/1‑93 (MQ=255)

         cccTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAtt                                                                                <  1:7796261/93‑1 (MQ=255)

          ccTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTg                                                                               <  1:6593887/93‑1 (MQ=255)

           cTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGc                                                                              >  1:2730324/1‑93 (MQ=255)

            tCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGCAATTCTGTCAATCCTTCGACCGCACCCAATATTGcc                                                                             >  1:880888/1‑93 (MQ=255)

            tCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGcc                                                                             >  2:3883336/1‑93 (MQ=255)

            tCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGcc                                                                             <  1:7864478/93‑1 (MQ=255)

              aaaCCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGc                                                                           <  2:7685550/93‑1 (MQ=255)

                 ccccAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTAt                                                                        >  2:3400861/1‑93 (MQ=255)

                  cccAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATc                                                                       >  1:6017838/1‑93 (MQ=255)

                   ccAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATca                                                                      <  1:6255697/93‑1 (MQ=255)

                   ccAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATca                                                                      <  1:397391/93‑1 (MQ=255)

                   ccAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATca                                                                      <  2:4096652/93‑1 (MQ=255)

                    caacaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcag                                                                     >  2:1952481/1‑93 (MQ=255)

                     aacaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagc                                                                    >  2:6906388/1‑93 (MQ=255)

                      acaaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagca                                                                   <  2:4662307/93‑1 (MQ=255)

                       caaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATcagcag                                                                  >  1:3622709/1‑93 (MQ=255)

                        aaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCagcagc                                                                 >  1:3367611/1‑93 (MQ=255)

                        aaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCagcagc                                                                 >  2:7377923/1‑93 (MQ=255)

                        aaGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCagcagc                                                                 <  2:4851405/93‑1 (MQ=255)

                          gTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGa                                                               >  1:955526/1‑93 (MQ=255)

                            gACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGt                                                             <  2:7654025/93‑1 (MQ=255)

                            gACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGt                                                             <  1:4227923/93‑1 (MQ=255)

                             aCCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTg                                                            >  2:1344780/1‑93 (MQ=255)

                             aCCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTg                                                            <  1:2164387/93‑1 (MQ=255)

                               cGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCa                                                          >  1:1688408/1‑93 (MQ=255)

                               cGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCa                                                          <  2:118296/93‑1 (MQ=255)

                                gACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCat                                                         <  1:4100245/93‑1 (MQ=255)

                                gACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCat                                                         <  1:3848906/93‑1 (MQ=255)

                                 aCAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCata                                                        >  2:6379643/1‑93 (MQ=255)

                                 aCAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCata                                                        >  1:4740336/1‑93 (MQ=255)

                                 aCAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCata                                                        >  1:3897080/1‑93 (MQ=255)

                                  cAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCatat                                                       <  1:3748008/93‑1 (MQ=255)

                                  cAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCatat                                                       <  1:6985007/93‑1 (MQ=255)

                                    ataatCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCg                                                     >  1:4542576/1‑93 (MQ=255)

                                      aatCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCt                                                   <  2:4390132/93‑1 (MQ=255)

                                       atCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtc                                                  <  1:1963530/93‑1 (MQ=255)

                                        tCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtca                                                 >  2:7082057/1‑93 (MQ=255)

                                        tCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtca                                                 <  2:5859958/93‑1 (MQ=255)

                                        tCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtca                                                 >  1:1198737/1‑93 (MQ=255)

                                        tCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtca                                                 <  1:3652151/93‑1 (MQ=255)

                                          atatGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCtcatc                                               >  2:94763/1‑93 (MQ=255)

                                            atGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCaa                                             <  1:4390027/93‑1 (MQ=255)

                                             tGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAAt                                            >  1:7178869/1‑93 (MQ=255)

                                             tGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGAATATCGCTCATCAAt                                            >  1:2342504/1‑93 (MQ=255)

                                                cccGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATaaa                                         <  2:4007183/93‑1 (MQ=255)

                                                cccGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATaaa                                         <  2:2795692/93‑1 (MQ=255)

                                                 ccGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAc                                        >  1:2423591/1‑93 (MQ=255)

                                                   gTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAccc                                      >  2:1739207/1‑93 (MQ=255)

                                                   gTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAccc                                      >  1:5566295/1‑93 (MQ=255)

                                                    tGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAcccc                                     >  1:2837636/1‑93 (MQ=255)

                                                      cTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTa                                   <  2:4731809/93‑1 (MQ=255)

                                                       tGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTaa                                  >  1:2252753/1‑93 (MQ=255)

                                                       tGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTaa                                  >  1:4429230/1‑93 (MQ=255)

                                                       tGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTaa                                  >  1:1019922/1‑93 (MQ=255)

                                                       tGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTaa                                  >  2:4536205/1‑93 (MQ=255)

                                                         gATGCCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGATATCAGCAGCGAGTGCATATCGCTCATCAATAACCCCCTAAat                                <  2:2915847/93‑1 (MQ=255)

                                                         gATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAat                                >  2:5127211/1‑93 (MQ=255)

                                                           tACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAatta                              >  2:7175691/1‑93 (MQ=255)

                                                           tACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAatta                              <  2:6826923/93‑1 (MQ=255)

                                                            aCCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAattat                             <  1:5704225/93‑1 (MQ=255)

                                                             ccGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAattatt                            <  1:3110323/93‑1 (MQ=255)

                                                             ccGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAattatt                            <  2:6249226/93‑1 (MQ=255)

                                                               ggCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTaa                          <  2:2456786/93‑1 (MQ=255)

                                                               ggCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTaa                          <  1:4991439/93‑1 (MQ=255)

                                                                 cAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTaaaa                        <  2:1517413/93‑1 (MQ=255)

                                                                 cAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTaaaa                        <  2:549491/93‑1 (MQ=255)

                                                                  aGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCCATAAACCCCTAAATTATTAAAAt                       >  2:4302140/1‑93 (MQ=255)

                                                                  aGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAAt                       <  1:3327794/93‑1 (MQ=255)

                                                                   gAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATg                      >  2:3535035/1‑93 (MQ=255)

                                                                    aaaTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGt                     <  1:1881454/93‑1 (MQ=255)

                                                                    aaaTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGt                     >  1:5115072/1‑93 (MQ=255)

                                                                     aaTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTa                    <  2:7514667/93‑1 (MQ=255)

                                                                     aaTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTa                    >  1:2673135/1‑93 (MQ=255)

                                                                     aaTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTa                    <  1:6350825/93‑1 (MQ=255)

                                                                      aTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAc                   >  1:7632242/1‑93 (MQ=255)

                                                                      aTTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAc                   <  1:354407/93‑1 (MQ=255)

                                                                       ttCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAcc                  >  1:5755845/1‑93 (MQ=255)

                                                                         cTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGc                <  2:3310481/93‑1 (MQ=255)

                                                                            tCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTg             >  1:3015389/1‑93 (MQ=255)

                                                                               aTCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTcc          <  2:2084084/93‑1 (MQ=255)

                                                                                tCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCg         >  2:268042/1‑93 (MQ=255)

                                                                                accTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCg         <  1:2474589/92‑1 (MQ=255)

                                                                                  cTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGaa       >  2:4997198/1‑93 (MQ=255)

                                                                                  cTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGaa       >  2:311080/1‑93 (MQ=255)

                                                                                  cTTAGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGaa       <  2:5344643/93‑1 (MQ=255)

                                                                                   ttCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAac      >  2:6542475/1‑93 (MQ=255)

                                                                                    tCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAact     >  2:1794985/1‑93 (MQ=255)

                                                                                       aCCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAactact  <  2:3688897/93‑1 (MQ=255)

GCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACT  >  NC_000913/3201639‑3201818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCG  >  NC_000913/3201636‑3201821

TTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCG                                                                                              >  SRR4302543.2975886/1‑94 (MQ=60)

TTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCG                                                                                              <  SRR4302543.1476795/94‑1 (MQ=60)

TTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCCGCAGAAATTCTGTAAATCCTCCGACCG                                                                                              >  SRR4302543.4098390/1‑94 (MQ=60)

   GCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCAC                                                                                           >  SRR4302543.7771648/1‑94 (MQ=60)

    CCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACC                                                                                          >  SRR4302543.4911964/1‑94 (MQ=60)

    CCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACC                                                                                          <  SRR4302543.4384280/94‑1 (MQ=60)

    CCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACC                                                                                          >  SRR4302543.476674/1‑94 (MQ=60)

     CGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCC                                                                                         >  SRR4302543.4641030/1‑94 (MQ=60)

     CGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCC                                                                                         >  SRR4302543.4984931/1‑94 (MQ=60)

     CGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCC                                                                                         <  SRR4302543.7482712/94‑1 (MQ=60)

      GTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCA                                                                                        <  SRR4302543.2441568/94‑1 (MQ=60)

      GTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCA                                                                                        >  SRR4302543.3719961/1‑94 (MQ=60)

       TGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAA                                                                                       >  SRR4302543.5945822/1‑94 (MQ=60)

       TGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAA                                                                                       >  SRR4302543.7406499/1‑94 (MQ=60)

        GTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAAT                                                                                      <  SRR4302543.1434002/94‑1 (MQ=60)

         TCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATA                                                                                     <  SRR4302543.2862880/94‑1 (MQ=60)

         TCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATA                                                                                     <  SRR4302543.2885736/94‑1 (MQ=60)

          CGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAT                                                                                    <  SRR4302543.7449507/94‑1 (MQ=60)

          CGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAT                                                                                    <  SRR4302543.5807042/94‑1 (MQ=60)

          CGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAT                                                                                    <  SRR4302543.326355/94‑1 (MQ=60)

          CGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAT                                                                                    >  SRR4302543.5649694/1‑94 (MQ=60)

          CGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATAT                                                                                    >  SRR4302543.4242154/1‑94 (MQ=60)

           GCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATT                                                                                   <  SRR4302543.7450403/94‑1 (MQ=60)

           GCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATT                                                                                   <  SRR4302543.5354310/94‑1 (MQ=60)

            CCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTG                                                                                  >  SRR4302543.168768/1‑94 (MQ=60)

            CCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTG                                                                                  <  SRR4302543.7869727/94‑1 (MQ=60)

             CCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGC                                                                                 >  SRR4302543.2755173/1‑94 (MQ=60)

             CCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGC                                                                                 <  SRR4302543.6656017/94‑1 (MQ=60)

              CTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGCAATTCTGTCAATCCTTCGACCGCACCCAATATTGCC                                                                                >  SRR4302543.888706/1‑94 (MQ=60)

              CTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCC                                                                                >  SRR4302543.37074/1‑94 (MQ=60)

              CTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCC                                                                                >  SRR4302543.37075/1‑94 (MQ=60)

              CTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCC                                                                                >  SRR4302543.888707/1‑94 (MQ=60)

               TCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCG                                                                               <  SRR4302543.7938539/94‑1 (MQ=60)

               CCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCG                                                                               <  SRR4302543.711366/94‑1 (MQ=60)

                CAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGC                                                                              >  SRR4302543.3753706/1‑94 (MQ=60)

                   ACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTAT                                                                           <  SRR4302543.2324064/94‑1 (MQ=60)

                   ACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTAT                                                                           >  SRR4302543.3378149/1‑94 (MQ=60)

                    CCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATC                                                                          >  SRR4302543.6073554/1‑94 (MQ=60)

                     CCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCA                                                                         <  SRR4302543.6509471/94‑1 (MQ=60)

                     CCCAACAAGTGACCGACAATAATCATAGGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCA                                                                         <  SRR4302543.543109/94‑1 (MQ=60)

                      CCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAG                                                                        <  SRR4302543.401063/94‑1 (MQ=60)

                      CCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAG                                                                        <  SRR4302543.6314706/94‑1 (MQ=60)

                       CAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGC                                                                       <  SRR4302543.4579319/94‑1 (MQ=60)

                        AACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCA                                                                      >  SRR4302543.2297039/1‑94 (MQ=60)

                         ACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAG                                                                     >  SRR4302543.4466708/1‑94 (MQ=60)

                         ACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAG                                                                     >  SRR4302543.3654630/1‑94 (MQ=60)

                          CAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGC                                                                    <  SRR4302543.6684680/94‑1 (MQ=60)

                          CAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGC                                                                    >  SRR4302543.2210809/1‑94 (MQ=60)

                          CAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGC                                                                    >  SRR4302543.3397868/1‑94 (MQ=60)

                          CAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACAGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGC                                                                    >  SRR4302543.7987461/1‑94 (MQ=60)

                           AAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCG                                                                   >  SRR4302543.3682504/1‑94 (MQ=60)

                            AGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGA                                                                  >  SRR4302543.963903/1‑94 (MQ=60)

                            AGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGA                                                                  <  SRR4302543.3353870/94‑1 (MQ=60)

                             GTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAG                                                                 <  SRR4302543.5645103/94‑1 (MQ=60)

                              TGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGT                                                                <  SRR4302543.6223189/94‑1 (MQ=60)

                              TGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGT                                                                <  SRR4302543.1238866/94‑1 (MQ=60)

                               GACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTG                                                               <  SRR4302543.4266279/94‑1 (MQ=60)

                                ACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGC                                                              <  SRR4302543.29731/94‑1 (MQ=60)

                                ACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGC                                                              <  SRR4302543.2183781/94‑1 (MQ=60)

                                 CCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCA                                                             <  SRR4302543.2709896/94‑1 (MQ=60)

                                 CCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCA                                                             >  SRR4302543.1703566/1‑94 (MQ=60)

                                 CCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCA                                                             >  SRR4302543.4007113/1‑94 (MQ=60)

                                  CGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCAT                                                            <  SRR4302543.57361/94‑1 (MQ=60)

                                  CGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCAT                                                            >  SRR4302543.3871428/1‑94 (MQ=60)

                                   GACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATA                                                           >  SRR4302543.4783650/1‑94 (MQ=60)

                                   GACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATA                                                           <  SRR4302543.4137098/94‑1 (MQ=60)

                                   GACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATA                                                           >  SRR4302543.1118274/1‑94 (MQ=60)

                                   GACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATA                                                           <  SRR4302543.3882904/94‑1 (MQ=60)

                                   GACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATA                                                           >  SRR4302543.3931495/1‑94 (MQ=60)

                                    ACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATAT                                                          <  SRR4302543.4845141/94‑1 (MQ=60)

                                    ACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATAT                                                          <  SRR4302543.2024501/94‑1 (MQ=60)

                                    ACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATAT                                                          <  SRR4302543.3625559/94‑1 (MQ=60)

                                     CAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATC                                                         <  SRR4302543.3780982/94‑1 (MQ=60)

                                     CAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATC                                                         <  SRR4302543.7050749/94‑1 (MQ=60)

                                     CAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATC                                                         >  SRR4302543.1863819/1‑94 (MQ=60)

                                      AATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCG                                                        >  SRR4302543.4583940/1‑94 (MQ=60)

                                          ATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCA                                                    >  SRR4302543.1959089/1‑94 (MQ=60)

                                          ATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCA                                                    <  SRR4302543.1981140/94‑1 (MQ=60)

                                          ATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCA                                                    >  SRR4302543.1209532/1‑94 (MQ=60)

                                           TCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCAT                                                   <  SRR4302543.3676849/94‑1 (MQ=60)

                                           TCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCAT                                                   <  SRR4302543.3973700/94‑1 (MQ=60)

                                           TCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCAT                                                   <  SRR4302543.3684236/94‑1 (MQ=60)

                                             ATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCA                                                 >  SRR4302543.4124257/1‑94 (MQ=60)

                                             ATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCA                                                 >  SRR4302543.937614/1‑94 (MQ=60)

                                               ATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAAT                                               >  SRR4302543.5311046/1‑94 (MQ=60)

                                               ATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAAT                                               >  SRR4302543.7248548/1‑94 (MQ=60)

                                               ATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAAT                                               <  SRR4302543.4429822/94‑1 (MQ=60)

                                               ATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGAATATCGCTCATCAAT                                               >  SRR4302543.2363753/1‑94 (MQ=60)

                                                TGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATA                                              <  SRR4302543.7390334/94‑1 (MQ=60)

                                                TGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATA                                              <  SRR4302543.4965361/94‑1 (MQ=60)

                                                 GGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAA                                             <  SRR4302543.4267765/94‑1 (MQ=60)

                                                   CCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAAC                                           >  SRR4302543.2445468/1‑94 (MQ=60)

                                                     CGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCC                                         >  SRR4302543.5616930/1‑94 (MQ=60)

                                                     CGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCC                                         <  SRR4302543.1913051/94‑1 (MQ=60)

                                                     CGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCC                                         >  SRR4302543.5616931/1‑94 (MQ=60)

                                                     CGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCC                                         >  SRR4302543.2568938/1‑94 (MQ=60)

                                                     CGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCC                                         >  SRR4302543.3900272/1‑94 (MQ=60)

                                                      GTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCC                                        <  SRR4302543.3445632/94‑1 (MQ=60)

                                                      GTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCC                                        >  SRR4302543.2493657/1‑94 (MQ=60)

                                                      GTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCC                                        >  SRR4302543.2863580/1‑94 (MQ=60)

                                                         CTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAA                                     >  SRR4302543.4469357/1‑94 (MQ=60)

                                                         CTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAA                                     >  SRR4302543.2273236/1‑94 (MQ=60)

                                                         CTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAA                                     >  SRR4302543.1028946/1‑94 (MQ=60)

                                                           GGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAAT                                   <  SRR4302543.98191/94‑1 (MQ=60)

                                                           GGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAAT                                   >  SRR4302543.1438638/1‑94 (MQ=60)

                                                           GGATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAAT                                   <  SRR4302543.3440897/94‑1 (MQ=60)

                                                            GATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATT                                  >  SRR4302543.5063538/1‑94 (MQ=60)

                                                            GATACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATT                                  <  SRR4302543.5244671/94‑1 (MQ=60)

                                                              TACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTAT                                <  SRR4302543.2420715/94‑1 (MQ=60)

                                                               ACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT                               <  SRR4302543.6397189/94‑1 (MQ=60)

                                                               ACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT                               >  SRR4302543.7713665/1‑94 (MQ=60)

                                                               ACCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT                               <  SRR4302543.5756252/94‑1 (MQ=60)

                                                                CCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTA                              <  SRR4302543.4071354/94‑1 (MQ=60)

                                                                CCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTA                              <  SRR4302543.5595837/94‑1 (MQ=60)

                                                                CCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTA                              >  SRR4302543.4796069/1‑94 (MQ=60)

                                                                CCGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTA                              <  SRR4302543.3138299/94‑1 (MQ=60)

                                                                 CGGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAA                             >  SRR4302543.5796073/1‑94 (MQ=60)

                                                                  GGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAA                            <  SRR4302543.5036877/94‑1 (MQ=60)

                                                                  GGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAA                            <  SRR4302543.2068237/94‑1 (MQ=60)

                                                                  GGCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAA                            <  SRR4302543.7119394/94‑1 (MQ=60)

                                                                   GCAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAA                           <  SRR4302543.816996/94‑1 (MQ=60)

                                                                    CAGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAAT                          <  SRR4302543.3393585/94‑1 (MQ=60)

                                                                     AGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATG                         <  SRR4302543.5714876/94‑1 (MQ=60)

                                                                     AGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATG                         <  SRR4302543.4475930/94‑1 (MQ=60)

                                                                     AGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATG                         >  SRR4302543.7904858/1‑94 (MQ=60)

                                                                     AGAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATG                         <  SRR4302543.3357808/94‑1 (MQ=60)

                                                                      GAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGT                        >  SRR4302543.5161347/1‑94 (MQ=60)

                                                                      GAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGT                        <  SRR4302543.7267297/94‑1 (MQ=60)

                                                                      GAAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGT                        >  SRR4302543.3910377/1‑94 (MQ=60)

                                                                       AAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTA                       <  SRR4302543.1898361/94‑1 (MQ=60)

                                                                       AAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTA                       <  SRR4302543.3612407/94‑1 (MQ=60)

                                                                       AAATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTA                       >  SRR4302543.2697371/1‑94 (MQ=60)

                                                                        AATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAC                      <  SRR4302543.6410538/94‑1 (MQ=60)

                                                                        AATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAC                      <  SRR4302543.6410539/94‑1 (MQ=60)

                                                                        AATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAC                      >  SRR4302543.7704661/1‑94 (MQ=60)

                                                                        AATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTAC                      <  SRR4302543.1293247/94‑1 (MQ=60)

                                                                         ATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACC                     <  SRR4302543.357679/94‑1 (MQ=60)

                                                                         ATTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACC                     >  SRR4302543.5808297/1‑94 (MQ=60)

                                                                          TTCTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCG                    <  SRR4302543.1735041/94‑1 (MQ=60)

                                                                            CTGTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCT                  >  SRR4302543.4176857/1‑94 (MQ=60)

                                                                              GTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTG                <  SRR4302543.2222200/94‑1 (MQ=60)

                                                                              GTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTG                >  SRR4302543.3042673/1‑94 (MQ=60)

                                                                              GTCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTG                >  SRR4302543.2468886/1‑94 (MQ=60)

                                                                               TCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGT               >  SRR4302543.700187/1‑94 (MQ=60)

                                                                               TCAATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGT               <  SRR4302543.7505926/94‑1 (MQ=60)

                                                                                 AATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCC             <  SRR4302543.5095271/94‑1 (MQ=60)

                                                                                  ATCCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCG            >  SRR4302543.7899955/1‑94 (MQ=60)

                                                                                   ACCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGA           <  SRR4302543.2496957/94‑1 (MQ=60)

                                                                                    CCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAA          >  SRR4302543.7413697/1‑94 (MQ=60)

                                                                                    CCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAA          <  SRR4302543.3827796/94‑1 (MQ=60)

                                                                                    CCTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAA          >  SRR4302543.2633003/1‑94 (MQ=60)

                                                                                     CTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAAC         <  SRR4302543.5942014/94‑1 (MQ=60)

                                                                                     CTTCGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAAC         >  SRR4302543.2793351/1‑94 (MQ=60)

                                                                                        CGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGACCTAC      >  SRR4302543.6192529/1‑94 (MQ=60)

                                                                                        CGACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTAC      <  SRR4302543.3076162/94‑1 (MQ=60)

                                                                                         GACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAANNGTACCGCTTGTNCNNANTNCN     >  SRR4302543.4653580/1‑94 (MQ=60)

                                                                                         GACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACT     >  SRR4302543.3748429/1‑94 (MQ=60)

                                                                                         GACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGAACCGCTTGTCCGAACTACT     >  SRR4302543.2702937/1‑94 (MQ=60)

                                                                                          ACCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTG    >  SRR4302543.5692695/1‑94 (MQ=60)

                                                                                           CCGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGC   <  SRR4302543.6226912/94‑1 (MQ=60)

                                                                                           CCGCACCCAATATTGCCGCTATCAGCAGCGAGGGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGC   >  SRR4302543.4962515/1‑94 (MQ=60)

                                                                                            CGCACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCG  <  SRR4302543.7574402/94‑1 (MQ=60)

TTCGCCGTGTCGCCCTCAAACCCCAACAAGTGACCGACAATAATCATATGGCCCGTGCTGGATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAATGTACCGCTTGTCCGAACTACTGCG  >  NC_000913/3201636‑3201821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`