Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,202,607	T→C	C91C (TGT→TGC)	ygiH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,202,607	0	T	C	99.5%	665.5 / ‑3.9	212	coding (273/618 nt)	ygiH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (102/109); minor base . (0/1); total (102/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

Only 100 of 219 total aligned reads displayed.

CGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGA  >  NC_000913/3202517‑3202696

cGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCgcctgcct                                                                                         <  1:3832872/93‑1 (MQ=255)

    gTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGa                                                                                     <  1:2770172/93‑1 (MQ=255)

     ttCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGac                                                                                    >  2:2871858/1‑93 (MQ=255)

     ttCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGac                                                                                    >  1:1736597/1‑93 (MQ=255)

        tGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGAcaca                                                                                 >  1:4272684/1‑93 (MQ=255)

        tGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGAcaca                                                                                 <  2:570755/93‑1 (MQ=255)

        tGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGAcaca                                                                                 >  1:1825168/1‑93 (MQ=255)

         gAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACAt                                                                                <  2:557300/93‑1 (MQ=255)

         gAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACAt                                                                                >  2:507768/1‑93 (MQ=255)

          aaaGGAATGTTGCCCGTTCGGGGGGCCTATGGATTAAGTGTCAACCCCCTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATc                                                                               <  1:1923890/93‑1 (MQ=255)

          aaaGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATc                                                                               <  1:3683908/93‑1 (MQ=255)

          aaaGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATc                                                                               <  2:2314743/93‑1 (MQ=255)

          aaaGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATc                                                                               <  2:2351727/93‑1 (MQ=255)

          aaaGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATc                                                                               >  2:865481/1‑93 (MQ=255)

             ggAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTgg                                                                            >  1:3230578/1‑93 (MQ=255)

             ggAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTgg                                                                            <  2:3107314/93‑1 (MQ=255)

             ggAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTgg                                                                            <  1:514314/93‑1 (MQ=255)

                aTGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACCTCTGGccc                                                                         >  2:3138207/1‑93 (MQ=255)

                aTGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGccc                                                                         >  2:1615138/1‑93 (MQ=255)

                aTGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGccc                                                                         >  2:2598322/1‑93 (MQ=255)

                  gTTGCCCGTCTGGGGCGCGCATGAATTAGGCGTCAGCCCCTTCTAGCTAGGCGTAATTCACATCGCCGCCTGCCTTGGACACATCTGGGCCGt                                                                       >  2:4276055/1‑93 (MQ=255)

                    tGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGttt                                                                     <  2:1389442/93‑1 (MQ=255)

                      cccGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTttc                                                                   <  1:2393300/93‑1 (MQ=255)

                      cccGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTttc                                                                   <  2:3029883/93‑1 (MQ=255)

                      cccGTCGGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTttc                                                                   <  2:4469412/93‑1 (MQ=255)

                       ccGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTttct                                                                  >  2:2967413/1‑93 (MQ=255)

                       ccGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTttct                                                                  >  1:3134933/1‑93 (MQ=255)

                        cGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTttctt                                                                 >  1:4521703/1‑93 (MQ=255)

                         gTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTttcttc                                                                >  1:810767/1‑93 (MQ=255)

                         gTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTttcttc                                                                <  1:3361679/93‑1 (MQ=255)

                         gTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTttcttc                                                                <  1:3504685/93‑1 (MQ=255)

                          tCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCg                                                               >  2:3336494/1‑93 (MQ=255)

                          tCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCg                                                               <  1:1171985/93‑1 (MQ=255)

                             ggggCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGAt                                                            >  1:2950657/1‑93 (MQ=255)

                              gggCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGAtt                                                           <  2:43018/93‑1 (MQ=255)

                               ggCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGAttt                                                          >  2:912396/1‑93 (MQ=255)

                                gcgcGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTa                                                         <  1:1592805/93‑1 (MQ=255)

                                   cgTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAag                                                      <  1:2535375/93‑1 (MQ=255)

                                    gTATTAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAagg                                                     <  2:3878007/93‑1 (MQ=255)

                                    gTATGAATTAGTTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAagg                                                     <  2:2646906/93‑1 (MQ=255)

                                    gTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAagg                                                     <  1:3568648/93‑1 (MQ=255)

                                           ttAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGaaaa                                              <  2:1512536/93‑1 (MQ=255)

                                            tAGGTGTCATCCCCTTCTGTCTAGGCTTAATTACCATCGCCTCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAg                                             <  1:1040628/93‑1 (MQ=255)

                                            tAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAg                                             <  1:1303229/93‑1 (MQ=255)

                                            tAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAg                                             <  2:1165538/93‑1 (MQ=255)

                                             aGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAgg                                            <  2:2077786/93‑1 (MQ=255)

                                             aGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAgg                                            <  2:766678/93‑1 (MQ=255)

                                              ggTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGc                                           >  2:1766056/1‑93 (MQ=255)

                                              ggTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGc                                           >  2:3244176/1‑93 (MQ=255)

                                              ggTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGc                                           >  1:1710875/1‑93 (MQ=255)

                                                tgtCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGTCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGt                                         <  2:2539864/93‑1 (MQ=255)

                                                 gtCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGtt                                        <  2:3986844/93‑1 (MQ=255)

                                                  tCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTg                                       <  1:3459933/93‑1 (MQ=255)

                                                    aGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCt                                     <  1:3188993/93‑1 (MQ=255)

                                                    aGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCt                                     >  2:294865/1‑93 (MQ=255)

                                                    aGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCt                                     <  1:1001471/93‑1 (MQ=255)

                                                    aGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTG‑CCTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTa                                    <  2:21769/93‑1 (MQ=255)

                                                     gCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTa                                    >  1:2091108/1‑93 (MQ=255)

                                                     gCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTa                                    >  1:4066934/1‑93 (MQ=255)

                                                     gCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTa                                    >  1:4179412/1‑93 (MQ=255)

                                                      ccccTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTAc                                   <  2:3636809/93‑1 (MQ=255)

                                                      ccccTTCTGGCTAGGCTTAATTGCCATCGCCACCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTAc                                   <  1:4684717/93‑1 (MQ=255)

                                                       cccTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTAcc                                  >  1:1672171/1‑93 (MQ=255)

                                                       cccTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTAcc                                  >  1:2380200/1‑93 (MQ=255)

                                                        ccTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCg                                 >  1:3248617/1‑93 (MQ=255)

                                                        ccTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCg                                 >  1:3922419/1‑93 (MQ=255)

                                                         cTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGc                                >  1:2736749/1‑93 (MQ=255)

                                                          ttCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCt                               >  1:1635194/1‑93 (MQ=255)

                                                          ttCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCt                               >  2:4476081/1‑93 (MQ=255)

                                                            cTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCttt                             <  1:2856056/93‑1 (MQ=255)

                                                             tGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCtttt                            <  2:709482/93‑1 (MQ=255)

                                                             tGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCtttt                            >  2:3082914/1‑93 (MQ=255)

                                                              ggCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTg                           <  1:2158538/93‑1 (MQ=255)

                                                               gCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTgg                          <  2:1931431/93‑1 (MQ=255)

                                                               gCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTgg                          <  2:1726493/93‑1 (MQ=255)

                                                               gCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCAGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTgg                          >  1:2268424/1‑93 (MQ=255)

                                                                 tAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTg                        <  1:2659728/93‑1 (MQ=255)

                                                                 tAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTg                        >  2:1695999/1‑93 (MQ=255)

                                                                  aGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGc                       >  1:4140045/1‑93 (MQ=255)

                                                                   ggCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGcc                      >  2:1052461/1‑93 (MQ=255)

                                                                   ggCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGcc                      >  2:2464354/1‑93 (MQ=255)

                                                                    gCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCa                     <  1:2408954/93‑1 (MQ=255)

                                                                     cTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGCAAAGGCGTTGCTACCGCTTTTGGTGCCAt                    >  2:605074/1‑93 (MQ=255)

                                                                      ttAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATc                   <  1:4184023/93‑1 (MQ=255)

                                                                      ttAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATc                   >  2:1233974/1‑93 (MQ=255)

                                                                      ttAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATc                   >  2:3571629/1‑93 (MQ=255)

                                                                            gCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCAccc             >  1:3036659/1‑93 (MQ=255)

                                                                             ccATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCa            >  2:4608277/1‑93 (MQ=255)

                                                                              cATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCAt           >  1:2072051/1‑93 (MQ=255)

                                                                               aTCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCAtt          <  2:3935857/93‑1 (MQ=255)

                                                                               aTCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCAtt          >  2:1002051/1‑93 (MQ=255)

                                                                                tCGCCGCCTGCCTTGGACACATCTGTCCCGTTTTCTTCGGATTTAAAGGAGGGAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTg         >  1:2283293/1‑93 (MQ=255)

                                                                                  gccgccTGCCTTGGACACATCTGGCCCTTTTTCTTCGGATTTAAAGGAGGAAAAGGCTTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGc       <  2:945660/93‑1 (MQ=255)

                                                                                   ccgccTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCt      <  1:830135/93‑1 (MQ=255)

                                                                                     gcctgcctTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTgg    <  1:4594339/93‑1 (MQ=255)

                                                                                     gcctgcctTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTgg    <  1:2613093/93‑1 (MQ=255)

                                                                                      ccTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTggg   >  1:876248/1‑93 (MQ=255)

                                                                                       cTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCCCCCATTGGCTGGGa  <  1:466177/93‑1 (MQ=255)

                                                                                       cTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGa  >  2:4149663/1‑93 (MQ=255)

                                                                                       cTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGa  <  2:2796863/93‑1 (MQ=255)

CGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGA  >  NC_000913/3202517‑3202696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTCGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGATCTC  >  NC_000913/3202514‑3202700

TTTCGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGC                                                                                               <  SRR4302535.479663/94‑1 (MQ=60)

 TTCGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCC                                                                                              >  SRR4302535.2517302/1‑94 (MQ=60)

 TTCGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCC                                                                                              >  SRR4302535.2824428/1‑94 (MQ=60)

  TCGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCT                                                                                             <  SRR4302535.4293648/94‑1 (MQ=60)

  TCGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCT                                                                                             <  SRR4302535.3893697/94‑1 (MQ=60)

   CGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTT                                                                                            <  SRR4302535.3859304/94‑1 (MQ=60)

    GACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTG                                                                                           <  SRR4302535.2342307/94‑1 (MQ=60)

       GTTCTGGAATGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGTTTAATTGCCATCGCCGCCTGCCTTGGAC                                                                                        <  SRR4302535.1249839/94‑1 (MQ=60)

       GTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGAC                                                                                        >  SRR4302535.1749150/1‑94 (MQ=60)

       GTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGAC                                                                                        <  SRR4302535.2789900/94‑1 (MQ=60)

       GTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGAC                                                                                        >  SRR4302535.3244423/1‑94 (MQ=60)

          CTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACA                                                                                     >  SRR4302535.1838344/1‑94 (MQ=60)

          CTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACA                                                                                     >  SRR4302535.4301932/1‑94 (MQ=60)

           TGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACAT                                                                                    >  SRR4302535.962318/1‑94 (MQ=60)

           TGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACAT                                                                                    >  SRR4302535.2108114/1‑94 (MQ=60)

            GAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATC                                                                                   >  SRR4302535.1060322/1‑94 (MQ=60)

             AAAGGAATGTTGCCCGTTCGGGGGGCCTATGGATTAAGTGTCAACCCCCTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCT                                                                                  <  SRR4302535.1937756/94‑1 (MQ=53)

             AAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCT                                                                                  <  SRR4302535.3709397/94‑1 (MQ=60)

              AAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTG                                                                                 <  SRR4302535.2752879/94‑1 (MQ=60)

              AAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTG                                                                                 <  SRR4302535.2721563/94‑1 (MQ=60)

               AGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGG                                                                                >  SRR4302535.3252975/1‑94 (MQ=60)

               AGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGG                                                                                >  SRR4302535.4177711/1‑94 (MQ=60)

                GGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGC                                                                               <  SRR4302535.517908/94‑1 (MQ=60)

                 GAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCC                                                                              >  SRR4302535.4342110/1‑94 (MQ=60)

                  AATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCC                                                                             >  SRR4302535.4718948/1‑94 (MQ=60)

                  AATGTTGCCCGTCTGGGGCGCGTATGAATTAGGGGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACAGCTGGCCG                                                                             >  SRR4302535.2608546/1‑94 (MQ=60)

                      TTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTT                                                                         <  SRR4302535.2986456/94‑1 (MQ=60)

                       TGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTT                                                                        >  SRR4302535.3192517/1‑94 (MQ=60)

                       TGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTT                                                                        >  SRR4302535.3564779/1‑94 (MQ=60)

                        GCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTC                                                                       >  SRR4302535.2674622/1‑94 (MQ=60)

                         CCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCT                                                                      >  SRR4302535.3156755/1‑94 (MQ=60)

                         CCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCT                                                                      <  SRR4302535.2410501/94‑1 (MQ=60)

                         CCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCT                                                                      >  SRR4302535.1673999/1‑94 (MQ=60)

                          CCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTT                                                                     <  SRR4302535.4566012/94‑1 (MQ=60)

                          CCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTT                                                                     >  SRR4302535.4552691/1‑94 (MQ=60)

                          CCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTT                                                                     >  SRR4302535.3440822/1‑94 (MQ=60)

                           CGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTC                                                                    >  SRR4302535.816435/1‑94 (MQ=60)

                            GTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCG                                                                   <  SRR4302535.3385073/94‑1 (MQ=60)

                            GTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCG                                                                   <  SRR4302535.3529040/94‑1 (MQ=60)

                             TCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGG                                                                  <  SRR4302535.2206712/94‑1 (MQ=60)

                             TCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGG                                                                  <  SRR4302535.1180355/94‑1 (MQ=60)

                             TCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGG                                                                  <  SRR4302535.4680423/94‑1 (MQ=60)

                               TGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGAT                                                                >  SRR4302535.2971386/1‑94 (MQ=60)

                                   GCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAA                                                            <  SRR4302535.1604301/94‑1 (MQ=60)

                                     GCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAG                                                          <  SRR4302535.2087486/94‑1 (MQ=60)

                                      CGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGG                                                         <  SRR4302535.2553638/94‑1 (MQ=60)

                                       GTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGA                                                        <  SRR4302535.3593393/94‑1 (MQ=60)

                                       GTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGA                                                        <  SRR4302535.4057071/94‑1 (MQ=60)

                                        TATGAATTAGGTGTCAGCCCCTTCTGGCTAGTCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAG                                                       <  SRR4302535.2401347/94‑1 (MQ=60)

                                        TATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAG                                                       <  SRR4302535.826471/94‑1 (MQ=60)

                                         ATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGG                                                      <  SRR4302535.1727040/94‑1 (MQ=60)

                                            AATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAA                                                   >  SRR4302535.781475/1‑94 (MQ=60)

                                             ATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAA                                                  <  SRR4302535.1631067/94‑1 (MQ=60)

                                              TTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAG                                                 <  SRR4302535.1030327/94‑1 (MQ=60)

                                               TAGGTGTCATCCCCTTCTGTCTAGGCTTAATTACCATCGCCTCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGG                                                <  SRR4302535.1048008/94‑1 (MQ=60)

                                               TAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGG                                                <  SRR4302535.470080/94‑1 (MQ=60)

                                               TAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGG                                                <  SRR4302535.1312533/94‑1 (MQ=60)

                                                AGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGC                                               >  SRR4302535.447903/1‑94 (MQ=60)

                                                AGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGC                                               >  SRR4302535.1723242/1‑94 (MQ=60)

                                                  GTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGT                                             <  SRR4302535.426971/94‑1 (MQ=60)

                                                   TGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTT                                            <  SRR4302535.2049708/94‑1 (MQ=60)

                                                     TCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGC                                          <  SRR4302535.3483989/94‑1 (MQ=60)

                                                      CAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCT                                         >  SRR4302535.2823478/1‑94 (MQ=60)

                                                       AGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTA                                        >  SRR4302535.3938240/1‑94 (MQ=60)

                                                       AGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTA                                        >  SRR4302535.2106177/1‑94 (MQ=60)

                                                       AGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTA                                        >  SRR4302535.4208017/1‑94 (MQ=60)

                                                       AGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTA                                        >  SRR4302535.4094763/1‑94 (MQ=60)

                                                       AGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTA                                        <  SRR4302535.3211045/94‑1 (MQ=60)

                                                       AGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTA                                        <  SRR4302535.1008587/94‑1 (MQ=60)

                                                       AGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTA                                        <  SRR4302535.3083053/94‑1 (MQ=60)

                                                         CCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACC                                      >  SRR4302535.1684274/1‑94 (MQ=60)

                                                         CCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACC                                      <  SRR4302535.840139/94‑1 (MQ=60)

                                                         CCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACC                                      <  SRR4302535.2286593/94‑1 (MQ=60)

                                                         CCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACC                                      <  SRR4302535.1530730/94‑1 (MQ=60)

                                                         CCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACC                                      >  SRR4302535.2397293/1‑94 (MQ=60)

                                                         CCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACC                                      <  SRR4302535.3791380/94‑1 (MQ=60)

                                                         CCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACC                                      <  SRR4302535.1802837/94‑1 (MQ=60)

                                                         CCCCTTCTGGCTAGGCTTAATTGCCATCGCCACCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACC                                      <  SRR4302535.4716417/94‑1 (MQ=60)

                                                          CCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCG                                     >  SRR4302535.3271184/1‑94 (MQ=60)

                                                          CCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCG                                     >  SRR4302535.3949230/1‑94 (MQ=60)

                                                           CCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGC                                    >  SRR4302535.2756271/1‑94 (MQ=60)

                                                           CCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGC                                    >  SRR4302535.2756272/1‑94 (MQ=60)

                                                           CCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGC                                    >  SRR4302535.3480998/1‑94 (MQ=60)

                                                            CTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCT                                   >  SRR4302535.1647009/1‑94 (MQ=60)

                                                               CTGGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTT                                <  SRR4302535.2876293/94‑1 (MQ=60)

                                                                 GGCTAGGCTTAATTGCTATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGG                              <  SRR4302535.1939196/94‑1 (MQ=60)

                                                                 GGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGG                              <  SRR4302535.2174047/94‑1 (MQ=60)

                                                                 GGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGG                              <  SRR4302535.3280565/94‑1 (MQ=60)

                                                                 GGCTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCAGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGG                              >  SRR4302535.2284595/1‑94 (MQ=60)

                                                                   CTAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTG                            <  SRR4302535.1683644/94‑1 (MQ=60)

                                                                    TAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGC                           <  SRR4302535.2678782/94‑1 (MQ=60)

                                                                    TAGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGC                           >  SRR4302535.4168393/1‑94 (MQ=60)

                                                                     AGGCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCC                          >  SRR4302535.3913765/1‑94 (MQ=60)

                                                                       GCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCAT                        <  SRR4302535.3511908/94‑1 (MQ=60)

                                                                       GCTTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCAT                        <  SRR4302535.2426297/94‑1 (MQ=60)

                                                                         TTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCG                      <  SRR4302535.4212651/94‑1 (MQ=60)

                                                                         TTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCG                      <  SRR4302535.534481/94‑1 (MQ=60)

                                                                         TTAATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCG                      >  SRR4302535.2916654/1‑94 (MQ=60)

                                                                            ATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCAC                   <  SRR4302535.4138360/94‑1 (MQ=60)

                                                                            ATTGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTCCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCAC                   >  SRR4302535.2525860/1‑94 (MQ=60)

                                                                              TGCCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCC                 >  SRR4302535.3057927/1‑94 (MQ=60)

                                                                                CCATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCAT               >  SRR4302535.2086983/1‑94 (MQ=60)

                                                                                 CATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATT              >  SRR4302535.242352/1‑94 (MQ=60)

                                                                                  ATCGCCGCCTGCCTTGGACATATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTG             <  SRR4302535.3147448/94‑1 (MQ=60)

                                                                                  ATCGCCGCCTGCCTTGGACACATCTGTCCCGTTTTCTTCGGATTTAAAGGAGGGAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTG             >  SRR4302535.2299607/1‑94 (MQ=60)

                                                                                  ATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTG             <  SRR4302535.3266550/94‑1 (MQ=60)

                                                                                  ATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTG             <  SRR4302535.186007/94‑1 (MQ=60)

                                                                                  ATCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTG             >  SRR4302535.1691448/1‑94 (MQ=60)

                                                                                   TCGCCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGG            <  SRR4302535.3617333/94‑1 (MQ=60)

                                                                                      CCGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTG         <  SRR4302535.835949/94‑1 (MQ=60)

                                                                                       CGCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGG        >  SRR4302535.2310851/1‑94 (MQ=60)

                                                                                        GCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGG       <  SRR4302535.4625604/94‑1 (MQ=60)

                                                                                        GCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGG       >  SRR4302535.882455/1‑94 (MQ=60)

                                                                                        GCCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGG       <  SRR4302535.2631836/94‑1 (MQ=60)

                                                                                         CCTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGA      >  SRR4302535.4208202/1‑94 (MQ=60)

                                                                                          CTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCCCCCATTGGCTGGGAT     <  SRR4302535.469440/94‑1 (MQ=60)

                                                                                          CTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGAT     >  SRR4302535.4489103/1‑94 (MQ=60)

                                                                                          CTGCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGAT     >  SRR4302535.2943106/1‑94 (MQ=60)

                                                                                            GCCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGATCT   <  SRR4302535.1470293/94‑1 (MQ=60)

                                                                                             CCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGATCTC  >  SRR4302535.1194434/1‑94 (MQ=60)

                                                                                             CCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGATCTC  >  SRR4302535.3739279/1‑94 (MQ=60)

                                                                                             CCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGATCTC  >  SRR4302535.950893/1‑94 (MQ=60)

                                                                                             CCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGATCTC  >  SRR4302535.2005613/1‑94 (MQ=60)

TTTCGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCGTTGCTACCGCTTTTGGTGCCATCGCACCCATTGGCTGGGATCTC  >  NC_000913/3202514‑3202700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`