Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,210,743	A→G	Q19R (CAA→CGA)	rpoD →	RNA polymerase sigma(70)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,210,743	0	A	G	99.6%	812.5 / ‑4.9	257	Q19R (CAA→CGA)	rpoD	RNA polymerase sigma(70)
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (124/132); total (124/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

Only 100 of 262 total aligned reads displayed.

CACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCA  >  NC_000913/3210653‑3210832

 acCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAgg                                                                                        >  2:1088944/1‑93 (MQ=255)

 acCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAgg                                                                                        >  1:1474465/1‑93 (MQ=255)

  ccACCCTCATGAAATAAGTGTGTATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGc                                                                                       <  2:1765757/93‑1 (MQ=255)

  ccAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGc                                                                                       <  1:4522153/93‑1 (MQ=255)

  ccAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGc                                                                                       >  1:4095213/1‑93 (MQ=255)

  ccAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGACACAGCTGAAACCTCTTGTCACTCGTGGTAAGGAGCGAGGc                                                                                       >  2:3230429/1‑93 (MQ=255)

   cAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCt                                                                                      >  1:159040/1‑93 (MQ=255)

   cAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCt                                                                                      >  1:3687325/1‑93 (MQ=255)

    aaCCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTa                                                                                     <  2:3973072/93‑1 (MQ=255)

    aaCCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTa                                                                                     >  1:1275285/1‑93 (MQ=255)

     aCCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTAt                                                                                    >  1:2291610/1‑93 (MQ=255)

      ccTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATc                                                                                   <  2:870884/93‑1 (MQ=255)

       cTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCt                                                                                  <  2:2174743/93‑1 (MQ=255)

        tCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTg                                                                                 >  1:4282638/1‑93 (MQ=255)

        tCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTg                                                                                 <  2:1174346/93‑1 (MQ=255)

         cATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGa                                                                                <  2:835064/93‑1 (MQ=255)

         cATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGa                                                                                <  2:2511039/93‑1 (MQ=255)

           tGAAATAAGTGTGGATACCGTCTTATGGAGCAGAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGAcc                                                                              >  1:4550163/1‑93 (MQ=255)

           tGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGAcc                                                                              <  1:2864383/93‑1 (MQ=255)

              aaTAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTAt                                                                           <  1:4010047/93‑1 (MQ=255)

               aTAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATg                                                                          >  2:3510055/1‑93 (MQ=255)

               aTAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATg                                                                          <  2:928539/93‑1 (MQ=255)

                tAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGc                                                                         <  1:4595729/93‑1 (MQ=255)

                tAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGc                                                                         >  2:3668671/1‑93 (MQ=255)

                tAAGAGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGc                                                                         <  1:575688/93‑1 (MQ=255)

                   gtgtGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGa                                                                      <  1:2025405/93‑1 (MQ=255)

                       ggATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTc                                                                  <  1:348015/93‑1 (MQ=255)

                        gATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCa                                                                 >  2:3492283/1‑93 (MQ=255)

                         aTACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCaa                                                                >  1:1826600/1‑93 (MQ=255)

                          tACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAAt                                                               >  1:1437018/1‑93 (MQ=255)

                            ccGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGa                                                             <  2:4488171/93‑1 (MQ=255)

                             cGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGAc                                                            >  2:1156442/1‑93 (MQ=255)

                               tCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCa                                                          >  2:2532087/1‑93 (MQ=255)

                               tCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCa                                                          >  1:178658/1‑93 (MQ=255)

                                 ttATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATc                                                        <  1:2401978/93‑1 (MQ=255)

                                 ttATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATc                                                        >  1:3605099/1‑93 (MQ=255)

                                 ttATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATc                                                        >  1:1636762/1‑93 (MQ=255)

                                  tATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCt                                                       <  1:3740185/93‑1 (MQ=255)

                                    tGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGc                                                     >  2:289338/1‑93 (MQ=255)

                                      gAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCg                                                   >  1:1834397/1‑93 (MQ=255)

                                      gAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCg                                                   >  2:4552121/1‑93 (MQ=255)

                                          aaaaCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAg                                               >  2:1933434/1‑93 (MQ=255)

                                          aaaaCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAg                                               >  2:1854116/1‑93 (MQ=255)

                                          aaaaCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAg                                               >  2:1637733/1‑93 (MQ=255)

                                          aaaaCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAg                                               >  2:1567080/1‑93 (MQ=255)

                                           aaaCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGa                                              <  1:2355838/93‑1 (MQ=255)

                                           aaaCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGa                                              <  1:2633712/93‑1 (MQ=255)

                                           aaaCCCGCAGTCACAGCTGAAACTTCTTGGCACGCGTGGGAAGGAGCGAGGCTATCTGACCTATTCCGAGGACAATGACCATCTTCCGGAAGa                                              >  2:2030576/1‑93 (MQ=255)

                                            aaCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGat                                             <  1:729156/93‑1 (MQ=255)

                                             aCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGata                                            <  2:514281/93‑1 (MQ=255)

                                             aCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGata                                            <  1:2262517/93‑1 (MQ=255)

                                             aCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGata                                            <  1:4460518/93‑1 (MQ=255)

                                              cccGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGatat                                           <  1:3007181/93‑1 (MQ=255)

                                               ccGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtc                                          >  1:2245802/1‑93 (MQ=255)

                                               ccGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtc                                          <  1:4372871/93‑1 (MQ=255)

                                               ccGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtc                                          >  1:1024816/1‑93 (MQ=255)

                                                 gCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtcgt                                        <  2:760217/93‑1 (MQ=255)

                                                 gCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATAtcgt                                        >  2:4659086/1‑93 (MQ=255)

                                                    gTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGa                                     >  2:4085896/1‑93 (MQ=255)

                                                     tCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGAt                                    <  1:4034053/93‑1 (MQ=255)

                                                      cacaGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGAtt                                   >  1:2133808/1‑93 (MQ=255)

                                                        caGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtca                                 <  2:3369248/93‑1 (MQ=255)

                                                          gCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcaga                               >  2:2330342/1‑93 (MQ=255)

                                                          gCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcaga                               <  2:1283651/93‑1 (MQ=255)

                                                            tGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatc                             >  2:3934270/1‑93 (MQ=255)

                                                            tGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatc                             <  2:3341705/93‑1 (MQ=255)

                                                             gAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatca                            >  1:3295021/1‑93 (MQ=255)

                                                             gAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatca                            >  1:656117/1‑93 (MQ=255)

                                                             gAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatca                            >  1:2181643/1‑93 (MQ=255)

                                                             gAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatca                            <  2:2697932/93‑1 (MQ=255)

                                                               aaCTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATtcagatcaga                          <  2:1905205/93‑1 (MQ=255)

                                                                aCTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTcagatcagat                         <  2:4274412/93‑1 (MQ=255)

                                                                aCTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTcagatcagat                         <  1:947228/93‑1 (MQ=255)

                                                                aCTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTcagatcagat                         <  2:704356/93‑1 (MQ=255)

                                                                 cttcttGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCagatcagatc                        >  1:3055736/1‑93 (MQ=255)

                                                                 cttcttGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCagatcagatc                        <  1:397384/93‑1 (MQ=255)

                                                                  ttcttGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCagatcagatca                       >  1:977687/1‑92 (MQ=255)

                                                                  ttcttGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCg                       >  2:1431320/1‑93 (MQ=255)

                                                                    tttGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGaa                     <  2:2415548/92‑1 (MQ=255)

                                                                     ttGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAg                    <  2:1242615/93‑1 (MQ=255)

                                                                     ttGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCCTCGATTCAGATCAGATCGAAg                    <  1:4647641/93‑1 (MQ=255)

                                                                     ttGTCACCCGTGGTAAGGAGCGAGGATATCTGACCTATGCCGAGGCCAATGACCATCTGCCGGAAGATATCGTCGATTGAGATCAGATCAAAg                    >  2:2079144/1‑93 (MQ=255)

                                                                       gTCACCCGTGGTAAGTAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAc                  <  2:3141009/93‑1 (MQ=255)

                                                                        tCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAca                 <  1:888379/93‑1 (MQ=255)

                                                                         cACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAcat                <  2:4424573/93‑1 (MQ=255)

                                                                           cccGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAcatca              <  2:3764024/93‑1 (MQ=255)

                                                                           cccGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAcatca              <  2:1776633/93‑1 (MQ=255)

                                                                            ccGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAcatcat             >  1:1326880/1‑93 (MQ=255)

                                                                             cGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACatcatc            <  2:2837084/93‑1 (MQ=255)

                                                                             cGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACatcatc            <  1:3580337/93‑1 (MQ=255)

                                                                              gTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATcc           >  1:1037019/1‑93 (MQ=255)

                                                                                 gTAAGGAGCGAGGGTATCTGACCTATGCGGAGGCCAATGACCATCTGCCGGAAGCTATCGTCGATTCACATCAGATCAAAGACATCATCCaat        >  2:661216/1‑92 (MQ=255)

                                                                                 gTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCaaa        >  1:1669964/1‑93 (MQ=255)

                                                                                 gTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCaaa        <  1:3154617/93‑1 (MQ=255)

                                                                                 gTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCaaa        <  2:2294635/93‑1 (MQ=255)

                                                                                    aGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGa     <  2:544906/93‑1 (MQ=255)

                                                                                     ggAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGAt    >  1:2517128/1‑93 (MQ=255)

                                                                                     ggAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGAt    <  2:2995192/93‑1 (MQ=255)

                                                                                      gAGCGAGGCTATCTGACGTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATc   <  2:4365095/93‑1 (MQ=255)

                                                                                      gAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATc   <  2:4134457/93‑1 (MQ=255)

CACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCA  >  NC_000913/3210653‑3210832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGA  >  NC_000913/3210651‑3210836

AACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGA                                                                                              >  SRR4302535.4536650/1‑94 (MQ=60)

  CACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAACCCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGG                                                                                            <  SRR4302535.3612896/94‑1 (MQ=60)

  CACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGG                                                                                            <  SRR4302535.3736603/94‑1 (MQ=60)

  CACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGG                                                                                            >  SRR4302535.1485022/1‑94 (MQ=60)

   ACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGC                                                                                           >  SRR4302535.4123239/1‑94 (MQ=60)

   ACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGC                                                                                           >  SRR4302535.2103976/1‑94 (MQ=60)

    CCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCT                                                                                          >  SRR4302535.3712838/1‑94 (MQ=60)

    CCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCT                                                                                          <  SRR4302535.4553141/94‑1 (MQ=60)

    CCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCT                                                                                          >  SRR4302535.160157/1‑94 (MQ=60)

     CAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTA                                                                                         >  SRR4302535.103259/1‑94 (MQ=60)

     CAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTA                                                                                         >  SRR4302535.1284391/1‑94 (MQ=60)

      AACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTAT                                                                                        <  SRR4302535.1476880/94‑1 (MQ=60)

      AACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTAT                                                                                        >  SRR4302535.3510210/1‑94 (MQ=60)

      AACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTAT                                                                                        >  SRR4302535.2308003/1‑94 (MQ=60)

       ACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATC                                                                                       <  SRR4302535.1460103/94‑1 (MQ=60)

         CTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTG                                                                                     <  SRR4302535.1422289/94‑1 (MQ=60)

         CTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTG                                                                                     <  SRR4302535.4028024/94‑1 (MQ=60)

         CTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTG                                                                                     >  SRR4302535.4311937/1‑94 (MQ=60)

           CATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGAC                                                                                   <  SRR4302535.4046583/94‑1 (MQ=60)

            ATGAAATAAGTGTGGATACCGTCTTATGGAGCAGAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACC                                                                                  >  SRR4302535.4581266/1‑94 (MQ=60)

            ATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACC                                                                                  >  SRR4302535.1104544/1‑94 (MQ=60)

             TGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCT                                                                                 <  SRR4302535.2884677/94‑1 (MQ=60)

               AAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTAT                                                                               >  SRR4302535.4428423/1‑94 (MQ=60)

               AAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTAT                                                                               <  SRR4302535.932097/94‑1 (MQ=60)

                AATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATG                                                                              <  SRR4302535.4037478/94‑1 (MQ=60)

                AATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATG                                                                              >  SRR4302535.2038001/1‑94 (MQ=60)

                 ATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGC                                                                             >  SRR4302535.2286927/1‑94 (MQ=60)

                  TAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCC                                                                            <  SRR4302535.4626998/94‑1 (MQ=60)

                  TAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCC                                                                            <  SRR4302535.1376208/94‑1 (MQ=60)

                  TAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCC                                                                            <  SRR4302535.1811871/94‑1 (MQ=60)

                  TAAGAGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCC                                                                            <  SRR4302535.579729/94‑1 (MQ=60)

                   AAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCG                                                                           >  SRR4302535.3447118/1‑94 (MQ=60)

                     GTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAG                                                                         <  SRR4302535.2040000/94‑1 (MQ=60)

                         GGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCA                                                                     <  SRR4302535.350412/94‑1 (MQ=60)

                          GATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAA                                                                    >  SRR4302535.1839786/1‑94 (MQ=60)

                           ATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAAT                                                                   >  SRR4302535.1447288/1‑94 (MQ=60)

                           ATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAAT                                                                   <  SRR4302535.1614248/94‑1 (MQ=60)

                            TACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATG                                                                  <  SRR4302535.1676303/94‑1 (MQ=60)

                            TACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATG                                                                  >  SRR4302535.801369/1‑94 (MQ=60)

                             ACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGA                                                                 >  SRR4302535.3104149/1‑94 (MQ=60)

                               CGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACC                                                               >  SRR4302535.245449/1‑94 (MQ=60)

                                GTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCA                                                              >  SRR4302535.179898/1‑94 (MQ=60)

                                GTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCA                                                              >  SRR4302535.1639663/1‑94 (MQ=60)

                                 TCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCAT                                                             <  SRR4302535.2378318/94‑1 (MQ=60)

                                  CTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATC                                                            >  SRR4302535.1648585/1‑94 (MQ=60)

                                  CTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATC                                                            >  SRR4302535.1843016/1‑94 (MQ=60)

                                  CTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATC                                                            >  SRR4302535.4119178/1‑94 (MQ=60)

                                  CTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATC                                                            <  SRR4302535.2446924/94‑1 (MQ=60)

                                  CTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATC                                                            >  SRR4302535.3630107/1‑94 (MQ=60)

                                   TTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCT                                                           <  SRR4302535.2419255/94‑1 (MQ=60)

                                   TTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCT                                                           <  SRR4302535.686534/94‑1 (MQ=60)

                                   TTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCT                                                           >  SRR4302535.3757515/1‑94 (MQ=60)

                                   TTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACATATGCCGAGGTCAATTACCATCT                                                           >  SRR4302535.2297901/1‑94 (MQ=60)

                                    TATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTG                                                          <  SRR4302535.2361502/94‑1 (MQ=60)

                                    TATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTG                                                          >  SRR4302535.2988429/1‑94 (MQ=60)

                                    TATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTG                                                          <  SRR4302535.3766071/94‑1 (MQ=60)

                                     ATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGC                                                         >  SRR4302535.2181820/1‑94 (MQ=60)

                                       GGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCG                                                       >  SRR4302535.1847634/1‑94 (MQ=60)

                                             AAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGAT                                                 <  SRR4302535.2652585/94‑1 (MQ=60)

                                             AAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGAT                                                 <  SRR4302535.2372730/94‑1 (MQ=60)

                                              AACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATA                                                <  SRR4302535.734270/94‑1 (MQ=60)

                                              AACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATA                                                >  SRR4302535.1839152/1‑94 (MQ=60)

                                               ACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATAT                                               <  SRR4302535.2278635/94‑1 (MQ=60)

                                               ACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATAT                                               <  SRR4302535.4491111/94‑1 (MQ=60)

                                               ACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATAT                                               <  SRR4302535.2249890/94‑1 (MQ=60)

                                               ACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATAT                                               <  SRR4302535.456811/94‑1 (MQ=60)

                                                CCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATC                                              <  SRR4302535.3028247/94‑1 (MQ=60)

                                                CCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATC                                              >  SRR4302535.3035474/1‑94 (MQ=60)

                                                CCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATC                                              <  SRR4302535.1783926/94‑1 (MQ=60)

                                                CCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATC                                              >  SRR4302535.1032096/1‑94 (MQ=60)

                                                CCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATC                                              >  SRR4302535.2261772/1‑94 (MQ=60)

                                                CCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATC                                              <  SRR4302535.45196/94‑1 (MQ=60)

                                                CCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATC                                              >  SRR4302535.653161/1‑94 (MQ=60)

                                                 CCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCG                                             <  SRR4302535.3088851/94‑1 (MQ=60)

                                                 CCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCG                                             <  SRR4302535.4402789/94‑1 (MQ=60)

                                                   CCAGTCACAGCTGAAACTTCTTGTCTCCCGTGTTAAGGAGCGATGCTATCTGACCTATGCCGATGTCAATTTCCATCTGCCGGAAGATATCGTC                                           <  SRR4302535.3545350/94‑1 (MQ=60)

                                                     AGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCATTGACCATCTGCCGGAAGATATCGTCGA                                         <  SRR4302535.640195/94‑1 (MQ=60)

                                                       TCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATT                                       <  SRR4302535.4061670/94‑1 (MQ=60)

                                                       TCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATT                                       >  SRR4302535.2149186/1‑94 (MQ=60)

                                                        CACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTC                                      >  SRR4302535.226044/1‑94 (MQ=60)

                                                         ACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGCCCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCA                                     <  SRR4302535.4440275/94‑1 (MQ=60)

                                                          CAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAG                                    <  SRR4302535.3814985/94‑1 (MQ=60)

                                                          CAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAG                                    <  SRR4302535.4674862/94‑1 (MQ=60)

                                                           AGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGA                                   >  SRR4302535.4191034/1‑94 (MQ=60)

                                                            GCTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGAT                                  >  SRR4302535.948641/1‑94 (MQ=60)

                                                             CTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATC                                 <  SRR4302535.3266927/94‑1 (MQ=60)

                                                             CTGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATC                                 <  SRR4302535.4590451/94‑1 (MQ=60)

                                                              TGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCA                                >  SRR4302535.660698/1‑94 (MQ=60)

                                                              TGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCA                                >  SRR4302535.2197204/1‑94 (MQ=60)

                                                              TGAAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCA                                >  SRR4302535.3317927/1‑94 (MQ=60)

                                                              TGAAACTTATTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCA                                <  SRR4302535.271529/94‑1 (MQ=60)

                                                                AAACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGA                              <  SRR4302535.2092579/94‑1 (MQ=60)

                                                                 AACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAT                             <  SRR4302535.2317876/94‑1 (MQ=60)

                                                                 AACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGAT                             >  SRR4302535.3001647/1‑94 (MQ=60)

                                                                  ACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATC                            >  SRR4302535.3077126/1‑94 (MQ=60)

                                                                  ACTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATC                            <  SRR4302535.953939/94‑1 (MQ=60)

                                                                   CTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCG                           <  SRR4302535.256168/94‑1 (MQ=60)

                                                                   CTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCG                           <  SRR4302535.2626649/94‑1 (MQ=60)

                                                                   CTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCG                           <  SRR4302535.400169/94‑1 (MQ=60)

                                                                   CTTCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCA                           >  SRR4302535.984633/1‑94 (MQ=60)

                                                                     TCTTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAA                         >  SRR4302535.3200531/1‑94 (MQ=60)

                                                                       TTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGA                       <  SRR4302535.1746962/94‑1 (MQ=60)

                                                                       TTGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCCTCGATTCAGATCAGATCGAAGA                       <  SRR4302535.4679123/94‑1 (MQ=60)

                                                                        TGTCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGAC                      >  SRR4302535.1888131/1‑94 (MQ=60)

                                                                          TCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACAT                    <  SRR4302535.894671/94‑1 (MQ=60)

                                                                          TCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACAT                    <  SRR4302535.156210/94‑1 (MQ=60)

                                                                          TCACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACAT                    <  SRR4302535.493698/94‑1 (MQ=60)

                                                                           CACCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATA                   >  SRR4302535.4015507/1‑94 (MQ=60)

                                                                             CCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCAT                 <  SRR4302535.4502796/94‑1 (MQ=60)

                                                                             CCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCAT                 <  SRR4302535.4379731/94‑1 (MQ=60)

                                                                             CCCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCAT                 >  SRR4302535.1336350/1‑94 (MQ=60)

                                                                              CCGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATC                <  SRR4302535.765466/94‑1 (MQ=60)

                                                                               CGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCC               <  SRR4302535.3605151/94‑1 (MQ=60)

                                                                               CGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCC               >  SRR4302535.1044385/1‑94 (MQ=60)

                                                                               CGTGGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCC               <  SRR4302535.2273099/94‑1 (MQ=60)

                                                                                  GGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAA            >  SRR4302535.1682049/1‑94 (MQ=60)

                                                                                  GGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAA            >  SRR4302535.4630050/1‑94 (MQ=60)

                                                                                  GGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAA            <  SRR4302535.4599925/94‑1 (MQ=60)

                                                                                  GGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAA            >  SRR4302535.4045889/1‑94 (MQ=60)

                                                                                  GGTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAA            >  SRR4302535.110841/1‑94 (MQ=60)

                                                                                   GTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAAT           <  SRR4302535.3176531/94‑1 (MQ=60)

                                                                                   GTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAAT           <  SRR4302535.132423/94‑1 (MQ=60)

                                                                                   GTAAGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAAT           <  SRR4302535.968157/94‑1 (MQ=60)

                                                                                      AGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGAT        >  SRR4302535.4263375/1‑94 (MQ=60)

                                                                                      AGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGAT        >  SRR4302535.2535272/1‑94 (MQ=60)

                                                                                      AGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGAT        <  SRR4302535.3671902/94‑1 (MQ=60)

                                                                                      AGGAGCGAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGAT        >  SRR4302535.3645458/1‑94 (MQ=60)

                                                                                            GAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGA  >  SRR4302535.311321/1‑94 (MQ=60)

                                                                                            GAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGA  >  SRR4302535.2509171/1‑94 (MQ=60)

AACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACCCGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATATCGTCGATTCAGATCAGATCGAAGACATCATCCAAATGATCAACGA  >  NC_000913/3210651‑3210836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`