Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,211,779 T→C R364R (CGT→CGC)  rpoD → RNA polymerase sigma(70)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,211,7790TC100.0% 648.0 / NA 201R364R (CGT→CGC) rpoDRNA polymerase sigma(70)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (90/111);  total (90/111)

Only 100 of 217 total aligned reads displayed.

TGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATA  >  NC_000913/3211689‑3211868
                                                                                          |                                                                                         
tGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAt                                                                                         <  1:3855651/93‑1 (MQ=255)
tGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAt                                                                                         >  2:573078/1‑93 (MQ=255)
tGAAGAAGAGCATCGCGCCTTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAt                                                                                         >  2:4574048/1‑93 (MQ=255)
 gaagaaGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATg                                                                                        <  2:342994/93‑1 (MQ=255)
   agaagTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTc                                                                                      >  1:1719250/1‑93 (MQ=255)
      agTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCAt                                                                                   <  1:1053141/93‑1 (MQ=255)
       gTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAACCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATc                                                                                  <  1:2910990/93‑1 (MQ=255)
          caccgcgcgCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGt                                                                               <  2:1762065/90‑1 (MQ=255)
          cATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGt                                                                               <  1:2270830/93‑1 (MQ=255)
            tCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGATAAAGATATCAACCGTCGCATGTCCATCGGTGa                                                                             >  2:1972333/1‑93 (MQ=255)
              gcgcCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAg                                                                           >  2:3845777/1‑93 (MQ=255)
               cgcCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGc                                                                          <  1:2335876/93‑1 (MQ=255)
               cgcCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGc                                                                          <  1:860357/93‑1 (MQ=255)
                gcCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCg                                                                         >  2:1168063/1‑93 (MQ=255)
                  ccTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaa                                                                       >  1:1627083/1‑93 (MQ=255)
                  ccTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaa                                                                       >  2:4327613/1‑93 (MQ=255)
                   cTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaaa                                                                      >  1:3045819/1‑93 (MQ=255)
                   cTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaaa                                                                      >  2:4281595/1‑93 (MQ=255)
                   cTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaaa                                                                      >  2:3638540/1‑93 (MQ=255)
                   cTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaaa                                                                      <  1:1542641/93‑1 (MQ=255)
                    tGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAg                                                                     <  2:1352583/93‑1 (MQ=255)
                      cAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGcc                                                                   >  2:1113149/1‑93 (MQ=255)
                        aaaaCTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCccg                                                                 >  1:4305229/1‑93 (MQ=255)
                         aaaCTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCccgc                                                                >  1:3139063/1‑93 (MQ=255)
                         aaaCTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCccgc                                                                <  1:190109/93‑1 (MQ=255)
                            cTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGt                                                             >  1:3395025/1‑93 (MQ=255)
                             tGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTg                                                            <  1:3974408/93‑1 (MQ=255)
                               cagcagATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCg                                                          >  2:3020614/1‑93 (MQ=255)
                                 gcagATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaa                                                        <  2:1579521/93‑1 (MQ=255)
                                 gcagATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaa                                                        >  1:1532615/1‑93 (MQ=255)
                                  cagATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaag                                                       <  2:2040420/93‑1 (MQ=255)
                                   agATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaaga                                                      >  2:2182157/1‑93 (MQ=255)
                                    gATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaagca                                                     >  1:2058877/1‑91 (MQ=255)
                                    gATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaagaa                                                     >  1:168991/1‑93 (MQ=255)
                                     aTTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaa                                                    >  1:3171662/1‑93 (MQ=255)
                                      ttGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaag                                                   >  1:4260813/1‑93 (MQ=255)
                                      ttGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaag                                                   >  1:3021351/1‑93 (MQ=255)
                                      ttGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaag                                                   <  1:2464438/93‑1 (MQ=255)
                                      ttGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaag                                                   <  1:679086/93‑1 (MQ=255)
                                       tGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaaga                                                  <  2:2499764/93‑1 (MQ=255)
                                       tGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaaga                                                  <  1:3420584/93‑1 (MQ=255)
                                         aagaagaaCCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAgaga                                                <  2:796273/93‑1 (MQ=255)
                                         aagaagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAgaga                                                >  2:2817239/1‑93 (MQ=255)
                                         aagaagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAgaga                                                <  1:2215766/93‑1 (MQ=255)
                                          agaagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGAt                                               <  2:3913790/93‑1 (MQ=255)
                                          agaagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGAt                                               >  1:2097124/1‑93 (MQ=255)
                                           gaagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATg                                              >  2:2289573/1‑93 (MQ=255)
                                            aagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATgg                                             <  1:59496/93‑1 (MQ=255)
                                               aaaCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTg                                          <  1:2376279/93‑1 (MQ=255)
                                                 aCCGGCCTGCCCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGaa                                        <  1:2545462/93‑1 (MQ=255)
                                                 aCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGaa                                        <  1:2237997/93‑1 (MQ=255)
                                                 aCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGaa                                        >  2:2021698/1‑93 (MQ=255)
                                                    ggCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCg                                     >  1:1263643/1‑93 (MQ=255)
                                                     gCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGa                                    >  2:3822531/1‑93 (MQ=255)
                                                     gCCTGACCATCGAGCAGCTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGa                                    <  2:2505866/93‑1 (MQ=255)
                                                     gCCTCACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGGTGAAGCGa                                    <  2:4543780/93‑1 (MQ=255)
                                                      ccTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGaa                                   <  1:1573036/93‑1 (MQ=255)
                                                       cTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAAc                                  <  1:2947632/93‑1 (MQ=255)
                                                       cTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAAc                                  <  2:2591372/93‑1 (MQ=255)
                                                        tGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACt                                 >  1:932504/1‑93 (MQ=255)
                                                          aCCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTa                               <  2:4441091/93‑1 (MQ=255)
                                                           ccATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTAc                              <  1:4535/93‑1 (MQ=255)
                                                           ccATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTAc                              >  1:3556539/1‑93 (MQ=255)
                                                            cATCGAGCAGGTTAAAGATATCAGCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACg                             <  2:3252589/93‑1 (MQ=255)
                                                            cATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACg                             <  2:1265594/93‑1 (MQ=255)
                                                            cATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACg                             >  2:4349018/1‑93 (MQ=255)
                                                            cATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACg                             >  1:2636836/1‑93 (MQ=255)
                                                              tCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTc                           >  1:534197/1‑93 (MQ=255)
                                                               cGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCt                          >  1:4478152/1‑93 (MQ=255)
                                                               cGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCt                          <  2:1201075/93‑1 (MQ=255)
                                                                gAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTg                         <  1:904546/93‑1 (MQ=255)
                                                                  gCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGt                       >  1:3536733/1‑93 (MQ=255)
                                                                  gCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGt                       >  2:2736461/1‑93 (MQ=255)
                                                                  gCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGt                       >  1:4084969/1‑93 (MQ=255)
                                                                   cAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGtt                      <  2:3608210/93‑1 (MQ=255)
                                                                    aGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTa                     <  2:3719394/93‑1 (MQ=255)
                                                                    aGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTa                     <  1:4050918/93‑1 (MQ=255)
                                                                    aGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTa                     >  2:2119510/1‑93 (MQ=255)
                                                                     ggTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTAt                    <  2:726168/93‑1 (MQ=255)
                                                                      gTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTAtt                   <  1:2394868/93‑1 (MQ=255)
                                                                       ttAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTAttt                  >  1:4206312/1‑93 (MQ=255)
                                                                         aaggATATCACGCGTCTCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCt                <  2:1098778/90‑1 (MQ=255)
                                                                          aaGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTa               <  2:1753213/93‑1 (MQ=255)
                                                                          aaGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTa               <  1:1772615/93‑1 (MQ=255)
                                                                           aGATCTCAACCGTCGCATGTCCATCGGTGAAGCAAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTAt              <  2:1820988/93‑1 (MQ=255)
                                                                           aGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTAt              >  1:4314608/1‑93 (MQ=255)
                                                                           aGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTAt              >  1:789267/1‑93 (MQ=255)
                                                                            gATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATc             <  2:712427/93‑1 (MQ=255)
                                                                              tatCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGc           >  1:757990/1‑93 (MQ=255)
                                                                                tCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTa         >  2:1672308/1‑93 (MQ=255)
                                                                                tCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTa         >  1:632669/1‑93 (MQ=255)
                                                                                 cagccGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTaa        <  1:1483145/90‑1 (MQ=255)
                                                                                 cAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTaa        <  2:4014005/93‑1 (MQ=255)
                                                                                  aaCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAg       <  2:3510566/93‑1 (MQ=255)
                                                                                  aaCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAg       <  2:1854034/93‑1 (MQ=255)
                                                                                  aaCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAg       >  1:3759523/1‑93 (MQ=255)
                                                                                   aCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGa      <  1:4161220/93‑1 (MQ=255)
                                                                                   aCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGa      <  2:94372/93‑1 (MQ=255)
                                                                                     cgtcgcATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGaaa    <  1:2406049/93‑1 (MQ=255)
                                                                                      gtcgcATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAAt   <  1:1856476/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATA  >  NC_000913/3211689‑3211868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  >  NC_000913/3211687‑3211872
                                                                                            |                                                                                             
TCTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATAAACCGTCGCA                                                                                              >  SRR4302535.1931831/1‑94 (MQ=60)
 CTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAT                                                                                             <  SRR4302535.2673551/94‑1 (MQ=60)
 CTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAT                                                                                             >  SRR4302535.650608/1‑94 (MQ=60)
  TGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATG                                                                                            <  SRR4302535.3882201/94‑1 (MQ=60)
   GAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGT                                                                                           >  SRR4302535.4362652/1‑94 (MQ=60)
    AAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTC                                                                                          <  SRR4302535.4526589/94‑1 (MQ=60)
    AAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTC                                                                                          >  SRR4302535.1731684/1‑94 (MQ=60)
        AGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATC                                                                                      <  SRR4302535.1060629/94‑1 (MQ=60)
        AGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATC                                                                                      <  SRR4302535.414055/94‑1 (MQ=60)
         GTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAACCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCG                                                                                     <  SRR4302535.2931551/94‑1 (MQ=60)
           GCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGT                                                                                   >  SRR4302535.2413683/1‑94 (MQ=60)
            CATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTG                                                                                  <  SRR4302535.2287022/94‑1 (MQ=60)
             ATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGA                                                                                 <  SRR4302535.868708/94‑1 (MQ=60)
              TCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAA                                                                                <  SRR4302535.3050366/94‑1 (MQ=60)
               CGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAG                                                                               <  SRR4302535.2236761/94‑1 (MQ=60)
                 CGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCG                                                                             <  SRR4302535.2352590/94‑1 (MQ=60)
                 CGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCG                                                                             <  SRR4302535.2368703/94‑1 (MQ=60)
                 CGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCG                                                                             <  SRR4302535.866431/94‑1 (MQ=60)
                 CGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCG                                                                             <  SRR4302535.293447/94‑1 (MQ=60)
                 CGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCG                                                                             <  SRR4302535.54342/94‑1 (MQ=60)
                  GCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGA                                                                            >  SRR4302535.166759/1‑94 (MQ=60)
                   CCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAA                                                                           <  SRR4302535.1967311/94‑1 (MQ=60)
                   CCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAA                                                                           >  SRR4302535.3854625/1‑94 (MQ=60)
                   CCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAA                                                                           >  SRR4302535.1638828/1‑94 (MQ=60)
                    CCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAA                                                                          >  SRR4302535.3067153/1‑94 (MQ=60)
                     CTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAG                                                                         <  SRR4302535.1553760/94‑1 (MQ=60)
                     CTGCAAAAACTGCAGCAGATAGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAG                                                                         <  SRR4302535.158884/94‑1 (MQ=60)
                        CAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCC                                                                      <  SRR4302535.4339488/94‑1 (MQ=60)
                         AAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCG                                                                     >  SRR4302535.4334683/1‑94 (MQ=60)
                          AAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGC                                                                    >  SRR4302535.3160907/1‑94 (MQ=60)
                          AAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGC                                                                    >  SRR4302535.2582933/1‑94 (MQ=60)
                           AAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCC                                                                   <  SRR4302535.191421/94‑1 (MQ=60)
                             ACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGT                                                                 >  SRR4302535.3418645/1‑94 (MQ=60)
                              CTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTG                                                                >  SRR4302535.1559589/1‑94 (MQ=60)
                               TGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGC                                                               <  SRR4302535.4001556/94‑1 (MQ=60)
                                  AGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAA                                                            >  SRR4302535.1543657/1‑94 (MQ=60)
                                  AGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAA                                                            <  SRR4302535.2397753/94‑1 (MQ=60)
                                     AGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGCA                                                         >  SRR4302535.2073710/1‑94 (MQ=60)
                                     AGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAA                                                         >  SRR4302535.170165/1‑94 (MQ=60)
                                     AGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAA                                                         <  SRR4302535.3432892/94‑1 (MQ=60)
                                     AGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAA                                                         <  SRR4302535.301571/94‑1 (MQ=60)
                                      GATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAA                                                        >  SRR4302535.3193620/1‑94 (MQ=60)
                                      GATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAA                                                        >  SRR4302535.391943/1‑94 (MQ=60)
                                       ATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAG                                                       >  SRR4302535.3042508/1‑94 (MQ=60)
                                       ATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAG                                                       >  SRR4302535.4289995/1‑94 (MQ=60)
                                        TTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGA                                                      >  SRR4302535.3293702/1‑94 (MQ=60)
                                        TTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGA                                                      <  SRR4302535.2482207/94‑1 (MQ=60)
                                        TTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGA                                                      <  SRR4302535.683838/94‑1 (MQ=60)
                                         TGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAG                                                     <  SRR4302535.4274323/94‑1 (MQ=60)
                                         TGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAG                                                     <  SRR4302535.3444373/94‑1 (MQ=60)
                                         TGAAGAAGAAACCGGCCTGACCAACGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAG                                                     <  SRR4302535.4356894/94‑1 (MQ=60)
                                          GAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGA                                                    <  SRR4302535.2775361/94‑1 (MQ=60)
                                          GAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGA                                                    >  SRR4302535.61050/1‑94 (MQ=60)
                                           AAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGAT                                                   >  SRR4302535.2112248/1‑94 (MQ=60)
                                           AAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGAT                                                   <  SRR4302535.2231543/94‑1 (MQ=60)
                                           AAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGAT                                                   <  SRR4302535.4306011/94‑1 (MQ=60)
                                            AGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATG                                                  >  SRR4302535.3904929/1‑94 (MQ=60)
                                            AGAAGAAACCGGCCTGACCACCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATG                                                  <  SRR4302535.2673421/94‑1 (MQ=60)
                                              AAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGT                                                <  SRR4302535.59934/94‑1 (MQ=60)
                                              AAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGT                                                <  SRR4302535.1745495/94‑1 (MQ=60)
                                                GAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTG                                              <  SRR4302535.3145398/94‑1 (MQ=60)
                                                GAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCACATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTG                                              >  SRR4302535.869618/1‑94 (MQ=60)
                                                 AAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGA                                             <  SRR4302535.2393349/94‑1 (MQ=60)
                                                   ACCGGCCTGCCCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAG                                           <  SRR4302535.2563786/94‑1 (MQ=60)
                                                   ACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAG                                           <  SRR4302535.2253920/94‑1 (MQ=60)
                                                    CCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGC                                          <  SRR4302535.2441246/94‑1 (MQ=60)
                                                     CGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCG                                         >  SRR4302535.1272684/1‑94 (MQ=60)
                                                     CGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCG                                         <  SRR4302535.4233713/94‑1 (MQ=60)
                                                     CGGCCTGACCATCGAACAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCG                                         <  SRR4302535.4315077/94‑1 (MQ=60)
                                                        CCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAAC                                      <  SRR4302535.1584389/94‑1 (MQ=60)
                                                         CTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACT                                     >  SRR4302535.939099/1‑94 (MQ=60)
                                                         CTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACT                                     <  SRR4302535.2968344/94‑1 (MQ=60)
                                                         CTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACT                                     >  SRR4302535.1550573/1‑94 (MQ=60)
                                                         CTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACT                                     >  SRR4302535.1884983/1‑94 (MQ=60)
                                                          TGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTT                                    >  SRR4302535.211246/1‑94 (MQ=60)
                                                            ACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTAC                                  >  SRR4302535.1312645/1‑94 (MQ=60)
                                                            ACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTAC                                  >  SRR4302535.3581208/1‑94 (MQ=60)
                                                            ACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTAC                                  <  SRR4302535.2004866/94‑1 (MQ=60)
                                                             CCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAATTTACG                                 >  SRR4302535.698187/1‑94 (MQ=60)
                                                             CCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACG                                 >  SRR4302535.2655725/1‑94 (MQ=60)
                                                             CCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACG                                 >  SRR4302535.2286143/1‑94 (MQ=60)
                                                             CCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACG                                 <  SRR4302535.1722440/94‑1 (MQ=60)
                                                             CCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACG                                 <  SRR4302535.4577/94‑1 (MQ=60)
                                                              CATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGT                                >  SRR4302535.845274/1‑94 (MQ=60)
                                                               ATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTC                               >  SRR4302535.537933/1‑94 (MQ=60)
                                                                TCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCT                              >  SRR4302535.4508898/1‑94 (MQ=60)
                                                                  GAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGG                            <  SRR4302535.910966/94‑1 (MQ=60)
                                                                  GAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGG                            <  SRR4302535.1606446/94‑1 (MQ=60)
                                                                   AGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGT                           >  SRR4302535.4112915/1‑94 (MQ=60)
                                                                   AGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGT                           >  SRR4302535.3561284/1‑94 (MQ=60)
                                                                    GCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTT                          <  SRR4302535.3391310/94‑1 (MQ=60)
                                                                    GCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTT                          <  SRR4302535.1788392/94‑1 (MQ=60)
                                                                      AGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTAT                        <  SRR4302535.2510385/94‑1 (MQ=60)
                                                                      AGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTAT                        <  SRR4302535.4078651/94‑1 (MQ=60)
                                                                        GTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTT                      >  SRR4302535.4235097/1‑94 (MQ=60)
                                                                        GTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTT                      <  SRR4302535.2412080/94‑1 (MQ=60)
                                                                        GTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTT                      <  SRR4302535.2110520/94‑1 (MQ=60)
                                                                        GTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTT                      <  SRR4302535.2352878/94‑1 (MQ=60)
                                                                         TTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTC                     >  SRR4302535.1499056/1‑94 (MQ=60)
                                                                          TAAAGATATCAACCGTCGCATGCCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCT                    <  SRR4302535.530733/94‑1 (MQ=60)
                                                                            AAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTAT                  >  SRR4302535.794808/1‑94 (MQ=60)
                                                                            AAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTAT                  <  SRR4302535.1785462/94‑1 (MQ=60)
                                                                            AAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTAT                  >  SRR4302535.4344122/1‑94 (MQ=60)
                                                                            AAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTAT                  <  SRR4302535.3553652/94‑1 (MQ=60)
                                                                             AGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATC                 <  SRR4302535.779036/94‑1 (MQ=60)
                                                                             AGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATC                 >  SRR4302535.4088847/1‑94 (MQ=60)
                                                                              GATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCG                <  SRR4302535.1082886/94‑1 (MQ=60)
                                                                               ATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGC               >  SRR4302535.763328/1‑94 (MQ=60)
                                                                                TATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCT              >  SRR4302535.229096/1‑94 (MQ=60)
                                                                                 ATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTttagagaaaaatacttttcgtttttttttttttt             >  SRR4302535.501517/1‑60 (MQ=60)
                                                                                 ATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTA             >  SRR4302535.637077/1‑94 (MQ=60)
                                                                                  TCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAA            <  SRR4302535.612593/94‑1 (MQ=60)
                                                                                   CAGCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           <  SRR4302535.1493751/94‑1 (MQ=60)
                                                                                   CAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           <  SRR4302535.4059698/94‑1 (MQ=60)
                                                                                   CAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           >  SRR4302535.3785510/1‑94 (MQ=60)
                                                                                     ACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAA         <  SRR4302535.923195/94‑1 (MQ=60)
                                                                                     ACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAA         <  SRR4302535.4189703/94‑1 (MQ=60)
                                                                                       CGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAAT       <  SRR4302535.316134/94‑1 (MQ=60)
                                                                                       CGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAAT       <  SRR4302535.2423367/94‑1 (MQ=60)
                                                                                        GTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATA      <  SRR4302535.3563130/94‑1 (MQ=60)
                                                                                        GTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATA      <  SRR4302535.1869846/94‑1 (MQ=60)
                                                                                         TCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATAC     <  SRR4302535.3615202/94‑1 (MQ=60)
                                                                                          CGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACA    >  SRR4302535.1202373/1‑94 (MQ=60)
                                                                                           GCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACAC   >  SRR4302535.3172086/1‑94 (MQ=60)
                                                                                           GCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACAC   <  SRR4302535.4022979/94‑1 (MQ=60)
                                                                                           GCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACAC   >  SRR4302535.4239655/1‑94 (MQ=60)
                                                                                           GCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACAC   >  SRR4302535.1855946/1‑94 (MQ=60)
                                                                                            CATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  <  SRR4302535.1870860/94‑1 (MQ=60)
                                                                                            |                                                                                             
TCTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  >  NC_000913/3211687‑3211872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑