Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,211,779 T→C R364R (CGT→CGC)  rpoD → RNA polymerase sigma(70)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,211,7790TC100.0% 1100.1 / NA 344R364R (CGT→CGC) rpoDRNA polymerase sigma(70)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (159/185);  total (159/185)

Only 100 of 375 total aligned reads displayed.

TGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATA  >  NC_000913/3211689‑3211868
                                                                                          |                                                                                         
tGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAt                                                                                         <  1:4840498/93‑1 (MQ=255)
 gaagaaGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATg                                                                                        >  1:4595639/1‑93 (MQ=255)
 gaagaaGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATg                                                                                        >  2:6948606/1‑93 (MQ=255)
  aagaagTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGt                                                                                       >  2:3551266/1‑93 (MQ=255)
   agaagTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTc                                                                                      >  1:1080784/1‑93 (MQ=255)
   agaagTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTc                                                                                      <  2:1204900/93‑1 (MQ=255)
    gaagTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTcc                                                                                     >  1:71493/1‑93 (MQ=255)
     aagTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCa                                                                                    <  1:6257415/93‑1 (MQ=255)
      agTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCAt                                                                                   <  2:74664/93‑1 (MQ=255)
      agTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCAt                                                                                   <  1:5244990/93‑1 (MQ=255)
       gTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATc                                                                                  >  1:2277994/1‑93 (MQ=255)
        tGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCg                                                                                 >  2:688005/1‑93 (MQ=255)
        tGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCg                                                                                 <  1:1000000/93‑1 (MQ=255)
         gCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCgg                                                                                >  2:367530/1‑93 (MQ=255)
          cATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGt                                                                               <  2:5045081/93‑1 (MQ=255)
            ccgcgCTCTGCAAATACTGCAGAAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGa                                                                             <  2:1551153/92‑1 (MQ=255)
             cgcgCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGaa                                                                            >  1:2487164/1‑93 (MQ=255)
                 cccTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGa                                                                        <  1:2998897/93‑1 (MQ=255)
                 cccTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGa                                                                        <  2:302610/93‑1 (MQ=255)
                  ccTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaa                                                                       >  2:21315/1‑93 (MQ=255)
                   cTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaaa                                                                      >  1:6334469/1‑93 (MQ=255)
                   cTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaaa                                                                      <  1:5971426/93‑1 (MQ=255)
                   cTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaaa                                                                      <  2:6981630/93‑1 (MQ=255)
                   cTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGaaa                                                                      <  1:4346911/93‑1 (MQ=255)
                    tGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAg                                                                     >  2:3975277/1‑93 (MQ=255)
                    tGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAg                                                                     >  1:2656556/1‑93 (MQ=255)
                    tGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAg                                                                     >  2:4982927/1‑93 (MQ=255)
                      cAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGcc                                                                   <  2:174520/93‑1 (MQ=255)
                       aaaaaCTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGccc                                                                  >  2:1703943/1‑93 (MQ=255)
                          aaCTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCccgcc                                                               <  2:5447648/93‑1 (MQ=255)
                            cTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGt                                                             <  2:6012096/93‑1 (MQ=255)
                             tGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGtc                                                            >  2:5782921/1‑92 (MQ=255)
                              gcagcaGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGc                                                           >  1:793584/1‑93 (MQ=255)
                                 gcagATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGCAGCGAAAGCCCGCCGTGCgaa                                                        >  2:7834748/1‑93 (MQ=255)
                                 gcagATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGCCCATCGGTGAAGCGAAAGCCCGCCGTGCgaa                                                        <  1:1827956/93‑1 (MQ=255)
                                  cagATTGAGGAATAAGCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaag                                                       <  1:7874017/93‑1 (MQ=255)
                                   agATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaaga                                                      >  2:2039283/1‑93 (MQ=255)
                                   agATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaaga                                                      <  1:7718999/93‑1 (MQ=255)
                                   agATTGAAGAAGAAACCGCCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaaga                                                      <  2:3087522/93‑1 (MQ=255)
                                    gATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCgaagaa                                                     >  2:5151873/1‑93 (MQ=255)
                                     aTTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaa                                                    <  1:4672879/93‑1 (MQ=255)
                                     aTTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaa                                                    <  1:1471612/93‑1 (MQ=255)
                                     aTTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaa                                                    <  1:6948607/93‑1 (MQ=255)
                                       tGAAGAAGAAGCCGGCGTTGCCGTTGAACAGTTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaaga                                                  <  1:7097080/93‑1 (MQ=255)
                                       tGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaaga                                                  >  1:671117/1‑93 (MQ=255)
                                       tGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaaga                                                  <  1:3261894/93‑1 (MQ=255)
                                       tGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaaga                                                  <  1:2774077/93‑1 (MQ=255)
                                       tGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGaagaaaga                                                  <  1:3584447/93‑1 (MQ=255)
                                         aagaagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAgaga                                                <  2:4267862/93‑1 (MQ=255)
                                         aagaagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAgaga                                                <  2:7897489/93‑1 (MQ=255)
                                          agaagaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAgagag                                               >  2:2329529/1‑92 (MQ=255)
                                              gaaACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGtt                                           >  1:615222/1‑93 (MQ=255)
                                                aaCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGa                                         <  2:1391460/93‑1 (MQ=255)
                                                 aCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGaa                                        <  2:2850984/93‑1 (MQ=255)
                                                 aCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGaa                                        >  1:3064427/1‑93 (MQ=255)
                                                 aCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGaa                                        >  2:6100328/1‑93 (MQ=255)
                                                   cGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGc                                      <  1:733777/93‑1 (MQ=255)
                                                   cGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGc                                      <  1:6461170/93‑1 (MQ=255)
                                                   cGGCCTGACCAGCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGc                                      <  1:4172258/93‑1 (MQ=255)
                                                    ggCCTGACCATCGAGCAGGTTAAAGATATCACCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCg                                     <  2:4384885/93‑1 (MQ=255)
                                                     gCCTGACCATCGAGCAGGTTAAAGATATCAACGGTCGCAGGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGa                                    >  2:2667398/1‑93 (MQ=255)
                                                        tGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACt                                 >  2:6625499/1‑93 (MQ=255)
                                                        tGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACt                                 >  2:2891453/1‑93 (MQ=255)
                                                        cgACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACt                                 <  1:2689941/92‑1 (MQ=255)
                                                         aaCCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACtt                                <  1:7473133/92‑1 (MQ=255)
                                                          aCCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATAGTTGAAGCGAACTTa                               <  2:3214439/93‑1 (MQ=255)
                                                           ctaacGAGCAGGTTAAAGATATCACCCGTCGAATGCCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTAc                              <  2:5611262/89‑1 (MQ=255)
                                                            cATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACg                             <  2:7705783/93‑1 (MQ=255)
                                                              tCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTc                           >  1:1631812/1‑93 (MQ=255)
                                                              tCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTc                           >  2:7291220/1‑93 (MQ=255)
                                                              tCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTc                           <  1:5759017/93‑1 (MQ=255)
                                                              tCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTc                           <  2:6223854/93‑1 (MQ=255)
                                                              tCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTc                           <  2:1335349/93‑1 (MQ=255)
                                                              tCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTc                           >  1:318704/1‑93 (MQ=255)
                                                               cGAGCAGGTTAAAGATATGCGCGGTCGCATGTCCAAAGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCt                          <  1:1271961/93‑1 (MQ=255)
                                                                gAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTg                         >  1:5681379/1‑93 (MQ=255)
                                                                 aGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTgg                        >  2:3850368/1‑93 (MQ=255)
                                                                 aGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTgg                        <  1:1549844/93‑1 (MQ=255)
                                                                  gCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGt                       >  2:1857054/1‑93 (MQ=255)
                                                                    aGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTa                     <  2:115019/93‑1 (MQ=255)
                                                                    aGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTa                     >  2:3417041/1‑93 (MQ=255)
                                                                      gTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTAtt                   >  2:4470985/1‑93 (MQ=255)
                                                                      gTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTAtt                   >  1:4863024/1‑93 (MQ=255)
                                                                       ttAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTAttt                  <  1:6100330/93‑1 (MQ=255)
                                                                        tAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTc                 <  1:3953667/93‑1 (MQ=255)
                                                                          aaGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTa               >  1:2025164/1‑93 (MQ=255)
                                                                           aGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTAt              >  2:4116365/1‑93 (MQ=255)
                                                                           aGATAGCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTAt              <  2:624641/93‑1 (MQ=255)
                                                                              tatCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGc           >  2:4750452/1‑93 (MQ=255)
                                                                              tatCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGc           >  2:4187074/1‑93 (MQ=255)
                                                                                 cAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTaa        <  1:5622895/93‑1 (MQ=255)
                                                                                   aCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGa      <  1:899777/93‑1 (MQ=255)
                                                                                   aCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGa      >  1:3404028/1‑93 (MQ=255)
                                                                                    ccGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGaa     <  1:4965658/93‑1 (MQ=255)
                                                                                    ccGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGaa     >  2:1289592/1‑93 (MQ=255)
                                                                                      gtcgcATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAAt   >  1:3862806/1‑93 (MQ=255)
                                                                                       tcggATGCCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATa  <  1:4526222/93‑1 (MQ=255)
                                                                                       tcgcATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATa  <  1:2399515/93‑1 (MQ=255)
                                                                                       tcgcATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATa  <  2:7096682/93‑1 (MQ=255)
                                                                                       tcgcATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATa  <  1:372014/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATA  >  NC_000913/3211689‑3211868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 206 total aligned reads displayed.
TCTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  >  NC_000913/3211687‑3211872
                                                                                            |                                                                                             
TCTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCA                                                                                              <  SRR4302543.5591546/94‑1 (MQ=60)
 CTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAT                                                                                             >  SRR4302543.7974207/1‑94 (MQ=60)
 CTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAT                                                                                             >  SRR4302543.7953287/1‑94 (MQ=60)
 CTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAT                                                                                             >  SRR4302543.278738/1‑94 (MQ=60)
 CTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAT                                                                                             <  SRR4302543.2855550/94‑1 (MQ=60)
 CTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAT                                                                                             <  SRR4302543.4234137/94‑1 (MQ=60)
 CTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCAT                                                                                             <  SRR4302543.4522452/94‑1 (MQ=60)
  TGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATG                                                                                            >  SRR4302543.308717/1‑94 (MQ=60)
  TGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATG                                                                                            >  SRR4302543.4637373/1‑94 (MQ=60)
  TGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATG                                                                                            <  SRR4302543.4884639/94‑1 (MQ=60)
   GAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGT                                                                                           <  SRR4302543.1625295/94‑1 (MQ=60)
    AAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTC                                                                                          >  SRR4302543.1090501/1‑94 (MQ=60)
    AAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTC                                                                                          <  SRR4302543.3104399/94‑1 (MQ=60)
    AAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTC                                                                                          >  SRR4302543.39170/1‑94 (MQ=60)
     AGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCC                                                                                         <  SRR4302543.1962904/94‑1 (MQ=60)
     AGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCC                                                                                         >  SRR4302543.2454514/1‑94 (MQ=60)
     AGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCC                                                                                         >  SRR4302543.72145/1‑94 (MQ=60)
       AAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCAT                                                                                       <  SRR4302543.6316438/94‑1 (MQ=60)
       AAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCAT                                                                                       <  SRR4302543.6461314/94‑1 (MQ=60)
       AAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCAT                                                                                       >  SRR4302543.747490/1‑94 (MQ=60)
        AGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATC                                                                                      <  SRR4302543.5292269/94‑1 (MQ=60)
        AGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATC                                                                                      >  SRR4302543.7174729/1‑94 (MQ=60)
        AGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATC                                                                                      >  SRR4302543.7174950/1‑94 (MQ=60)
        AGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATC                                                                                      >  SRR4302543.2298828/1‑94 (MQ=60)
         GTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCG                                                                                     <  SRR4302543.5470527/94‑1 (MQ=60)
         ATGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCG                                                                                     <  SRR4302543.5702579/94‑1 (MQ=60)
          TGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGG                                                                                    >  SRR4302543.7454356/1‑94 (MQ=60)
          TGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGG                                                                                    <  SRR4302543.1008831/94‑1 (MQ=60)
           GCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGT                                                                                   <  SRR4302543.3358327/94‑1 (MQ=60)
           GCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGT                                                                                   <  SRR4302543.2572812/94‑1 (MQ=60)
            CATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTG                                                                                  <  SRR4302543.7639694/94‑1 (MQ=60)
             ATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGA                                                                                 >  SRR4302543.46945/1‑94 (MQ=60)
             ATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGA                                                                                 >  SRR4302543.5689305/1‑94 (MQ=60)
             ATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGA                                                                                 >  SRR4302543.5803511/1‑94 (MQ=60)
             ATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGA                                                                                 >  SRR4302543.4886163/1‑94 (MQ=60)
              TCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAA                                                                                <  SRR4302543.6218812/94‑1 (MQ=60)
              TCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAA                                                                                >  SRR4302543.2509702/1‑94 (MQ=60)
                GCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGC                                                                              <  SRR4302543.806953/94‑1 (MQ=60)
                 CGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCG                                                                             <  SRR4302543.5944386/94‑1 (MQ=60)
                  GCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGA                                                                            >  SRR4302543.7637490/1‑94 (MQ=60)
                   CCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAA                                                                           <  SRR4302543.1465132/94‑1 (MQ=60)
                   CCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAA                                                                           <  SRR4302543.3026090/94‑1 (MQ=60)
                   CCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAA                                                                           >  SRR4302543.598389/1‑94 (MQ=60)
                   CCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAA                                                                           <  SRR4302543.2370787/94‑1 (MQ=60)
                   CCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAA                                                                           >  SRR4302543.2080038/1‑94 (MQ=60)
                    CCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAA                                                                          >  SRR4302543.6394042/1‑94 (MQ=60)
                     CTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAG                                                                         <  SRR4302543.6026115/94‑1 (MQ=60)
                     CTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAG                                                                         <  SRR4302543.4386318/94‑1 (MQ=60)
                     CTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAG                                                                         <  SRR4302543.7858596/94‑1 (MQ=60)
                     CTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAG                                                                         >  SRR4302543.2680639/1‑94 (MQ=60)
                        CAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCCACCGTCGCATGTTCATCGGTGAAGCGAAAGCCC                                                                      >  SRR4302543.7710809/1‑94 (MQ=60)
                        CAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCC                                                                      >  SRR4302543.7440260/1‑94 (MQ=60)
                        CAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCC                                                                      <  SRR4302543.5133865/94‑1 (MQ=60)
                        CAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCC                                                                      >  SRR4302543.485270/1‑94 (MQ=60)
                        CAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCC                                                                      >  SRR4302543.6635385/1‑94 (MQ=60)
                         AAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCAG                                                                     >  SRR4302543.3197085/1‑94 (MQ=60)
                            AACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCG                                                                  <  SRR4302543.3101774/94‑1 (MQ=60)
                             ACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGT                                                                 <  SRR4302543.1305133/94‑1 (MQ=60)
                             ACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGT                                                                 <  SRR4302543.2686570/94‑1 (MQ=60)
                               TGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGC                                                               >  SRR4302543.800560/1‑94 (MQ=60)
                               TGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCAAAAGCCGGCCGTGC                                                               >  SRR4302543.2674502/1‑94 (MQ=60)
                                  AGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAA                                                            <  SRR4302543.2704376/94‑1 (MQ=60)
                                  AGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAA                                                            <  SRR4302543.2704374/94‑1 (MQ=60)
                                   GCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAG                                                           <  SRR4302543.3852609/94‑1 (MQ=60)
                                   GCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGCCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAG                                                           <  SRR4302543.1844371/94‑1 (MQ=60)
                                    CAGATTGAGGAATAAGCCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGA                                                          <  SRR4302543.7948143/94‑1 (MQ=60)
                                    CAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGA                                                          >  SRR4302543.2474700/1‑94 (MQ=60)
                                     AGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAA                                                         <  SRR4302543.6444429/94‑1 (MQ=60)
                                     AGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAA                                                         <  SRR4302543.2859813/94‑1 (MQ=60)
                                     AGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAA                                                         >  SRR4302543.1743158/1‑94 (MQ=60)
                                     AGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAA                                                         <  SRR4302543.7791994/94‑1 (MQ=60)
                                     AGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAA                                                         <  SRR4302543.3534344/94‑1 (MQ=60)
                                       ATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAG                                                       <  SRR4302543.1484866/94‑1 (MQ=60)
                                       ATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAG                                                       <  SRR4302543.4715477/94‑1 (MQ=60)
                                       ATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAG                                                       >  SRR4302543.1519671/1‑94 (MQ=60)
                                       ATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAG                                                       <  SRR4302543.4359032/94‑1 (MQ=60)
                                       ATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAG                                                       <  SRR4302543.7013978/94‑1 (MQ=60)
                                       ATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAG                                                       <  SRR4302543.1140933/94‑1 (MQ=60)
                                       ATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAG                                                       <  SRR4302543.1904026/94‑1 (MQ=60)
                                        TTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGA                                                      <  SRR4302543.5282021/94‑1 (MQ=60)
                                        TTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGA                                                      <  SRR4302543.6201898/94‑1 (MQ=60)
                                        TTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGA                                                      >  SRR4302543.677291/1‑94 (MQ=60)
                                         tgaagaagaagccggcgttgccgttgaacagtTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAG                                                     <  SRR4302543.7165996/62‑1 (MQ=60)
                                         TGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAG                                                     <  SRR4302543.3306597/94‑1 (MQ=60)
                                         TGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAG                                                     <  SRR4302543.3616105/94‑1 (MQ=60)
                                         TGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAG                                                     <  SRR4302543.3291374/94‑1 (MQ=60)
                                         TGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAG                                                     <  SRR4302543.2799331/94‑1 (MQ=60)
                                         TGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAG                                                     <  SRR4302543.7165997/94‑1 (MQ=60)
                                          GAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGA                                                    <  SRR4302543.4569793/94‑1 (MQ=60)
                                          GAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGA                                                    <  SRR4302543.922047/94‑1 (MQ=60)
                                           AAGAAGAAACCGGCCTGACCATCGAGCAGGTTTAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGAT                                                   <  SRR4302543.3941162/94‑1 (MQ=60)
                                           AAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGAT                                                   <  SRR4302543.7916254/94‑1 (MQ=60)
                                           AAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGAT                                                   <  SRR4302543.1596407/94‑1 (MQ=60)
                                            AGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCACCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATG                                                  <  SRR4302543.4590576/94‑1 (MQ=60)
                                            AGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATG                                                  <  SRR4302543.4428277/94‑1 (MQ=60)
                                             GAAGGACCCGGCCTGACCATCGGGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGG                                                 <  SRR4302543.5395743/94‑1 (MQ=60)
                                              AAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGT                                                <  SRR4302543.1237701/94‑1 (MQ=60)
                                              AAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGT                                                >  SRR4302543.2855644/1‑94 (MQ=60)
                                               AGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTT                                               >  SRR4302543.5906509/1‑94 (MQ=60)
                                               AGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTT                                               >  SRR4302543.620847/1‑94 (MQ=60)
                                               AGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTT                                               >  SRR4302543.4904191/1‑94 (MQ=60)
                                                GAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTG                                              <  SRR4302543.1062753/94‑1 (MQ=60)
                                                GAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTG                                              >  SRR4302543.6919394/1‑94 (MQ=60)
                                                 AAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGA                                             <  SRR4302543.3704284/94‑1 (MQ=60)
                                                  AACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAA                                            >  SRR4302543.34991/1‑94 (MQ=60)
                                                  AACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAA                                            >  SRR4302543.3091991/1‑94 (MQ=60)
                                                  AACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAA                                            >  SRR4302543.6428868/1‑94 (MQ=60)
                                                  AACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACAGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAA                                            >  SRR4302543.3497794/1‑94 (MQ=60)
                                                   ACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAG                                           <  SRR4302543.3287772/94‑1 (MQ=60)
                                                     CGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCG                                         <  SRR4302543.6158878/94‑1 (MQ=60)
                                                     CGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCG                                         <  SRR4302543.740349/94‑1 (MQ=60)
                                                     CGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCG                                         <  SRR4302543.6522049/94‑1 (MQ=60)
                                                     CGGCCTGACCAGCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCG                                         <  SRR4302543.4210040/94‑1 (MQ=60)
                                                      GGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGA                                        >  SRR4302543.577954/1‑94 (MQ=60)
                                                      GGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGA                                        >  SRR4302543.4830856/1‑94 (MQ=60)
                                                       GCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAA                                       >  SRR4302543.715004/1‑94 (MQ=60)
                                                       GCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAA                                       <  SRR4302543.4908967/94‑1 (MQ=60)
                                                       GCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAA                                       <  SRR4302543.7130512/94‑1 (MQ=60)
                                                        GCTGACCATCGAGCAGGTTAAAGATATCAGCCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAAC                                      <  SRR4302543.4271993/94‑1 (MQ=60)
                                                        CCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAAC                                      >  SRR4302543.341862/1‑94 (MQ=60)
                                                        CCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAAC                                      <  SRR4302543.3057081/94‑1 (MQ=60)
                                                        CCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAAC                                      <  SRR4302543.6346407/94‑1 (MQ=60)
                                                        CCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTAAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAAC                                      <  SRR4302543.4271994/94‑1 (MQ=60)
                                                         CTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACT                                     >  SRR4302543.2973331/1‑94 (MQ=60)
                                                         CTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACT                                     >  SRR4302543.6328316/1‑94 (MQ=60)
                                                         CTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACT                                     >  SRR4302543.2727634/1‑94 (MQ=60)
                                                         CTGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACT                                     >  SRR4302543.3626312/1‑94 (MQ=60)
                                                          TGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTT                                    <  SRR4302543.3976903/94‑1 (MQ=60)
                                                          TGACCAACGAGCAGGTTAAAGATATCAACCTTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGATCTT                                    <  SRR4302543.2714331/94‑1 (MQ=60)
                                                          CGACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTT                                    <  SRR4302543.2714332/94‑1 (MQ=60)
                                                           GACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTG                                   >  SRR4302543.2057622/1‑94 (MQ=60)
                                                           AACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTA                                   <  SRR4302543.7544691/94‑1 (MQ=60)
                                                            ACCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTAC                                  <  SRR4302543.3995853/94‑1 (MQ=60)
                                                             CCATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACG                                 <  SRR4302543.6620576/94‑1 (MQ=60)
                                                              CATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGC                                >  SRR4302543.610839/1‑94 (MQ=60)
                                                               ATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGCACTTACGTC                               >  SRR4302543.809090/1‑94 (MQ=60)
                                                               ATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTC                               >  SRR4302543.809096/1‑94 (MQ=60)
                                                               ATCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTC                               >  SRR4302543.1646359/1‑94 (MQ=60)
                                                                TCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCT                              <  SRR4302543.5811487/94‑1 (MQ=60)
                                                                 CGAGCAGGTTAAAGATATGCGCGGTCGCATGTCCAAAGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTG                             <  SRR4302543.1283324/94‑1 (MQ=60)
                                                                 CGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTG                             >  SRR4302543.5733238/1‑94 (MQ=60)
                                                                 CGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTG                             <  SRR4302543.5644429/94‑1 (MQ=60)
                                                                 CGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTAAGTCTG                             >  SRR4302543.2676778/1‑94 (MQ=60)
                                                                 CGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATAGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTG                             <  SRR4302543.1283325/94‑1 (MQ=60)
                                                                   AGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGT                           <  SRR4302543.1563705/94‑1 (MQ=60)
                                                                    GCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTT                          >  SRR4302543.4072978/1‑94 (MQ=60)
                                                                    GCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTT                          <  SRR4302543.3289538/94‑1 (MQ=60)
                                                                     CAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTA                         >  SRR4302543.7938998/1‑94 (MQ=60)
                                                                     CAGGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTA                         >  SRR4302543.6308002/1‑94 (MQ=60)
                                                                       GGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATT                       >  SRR4302543.4907359/1‑94 (MQ=60)
                                                                       GGTTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATT                       >  SRR4302543.108127/1‑94 (MQ=60)
                                                                         TTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTC                     <  SRR4302543.6158127/94‑1 (MQ=60)
                                                                         TTAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTC                     <  SRR4302543.6158126/94‑1 (MQ=60)
                                                                          TAAAGATATCAGCCGTCGCATGTCCATCGGTGAAGCGAAAGCACGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCT                    <  SRR4302543.633470/94‑1 (MQ=60)
                                                                          TAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCT                    <  SRR4302543.7391726/94‑1 (MQ=60)
                                                                          TAAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCT                    <  SRR4302543.3988655/94‑1 (MQ=60)
                                                                           AAAGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTA                   >  SRR4302543.2043365/1‑94 (MQ=60)
                                                                           AAAGAGATCAACCGTCGCATGTCCATCGGTCAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTA                   <  SRR4302543.6786322/94‑1 (MQ=60)
                                                                             AGATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATC                 <  SRR4302543.1526956/94‑1 (MQ=60)
                                                                              GATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCG                <  SRR4302543.31/94‑1 (MQ=60)
                                                                              GATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCG                >  SRR4302543.1755669/1‑94 (MQ=60)
                                                                              GATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCG                <  SRR4302543.7027205/94‑1 (MQ=60)
                                                                               ATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGC               >  SRR4302543.3881194/1‑94 (MQ=60)
                                                                               ATATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGC               >  SRR4302543.4662492/1‑94 (MQ=60)
                                                                                TATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCT              >  SRR4302543.4882048/1‑94 (MQ=60)
                                                                                 ATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTA             >  SRR4302543.4954329/1‑94 (MQ=60)
                                                                                 ATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTA             <  SRR4302543.1780763/94‑1 (MQ=60)
                                                                                 ATCAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTA             >  SRR4302543.4712487/1‑94 (MQ=60)
                                                                                   CAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           >  SRR4302543.2475460/1‑94 (MQ=60)
                                                                                   CAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           <  SRR4302543.2005143/94‑1 (MQ=60)
                                                                                   CAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           >  SRR4302543.4003637/1‑94 (MQ=60)
                                                                                   CAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           <  SRR4302543.4589790/94‑1 (MQ=60)
                                                                                   CAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           <  SRR4302543.5674137/94‑1 (MQ=60)
                                                                                   CAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           <  SRR4302543.6300366/94‑1 (MQ=60)
                                                                                   CAACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAG           >  SRR4302543.1258838/1‑94 (MQ=60)
                                                                                    AACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGA          >  SRR4302543.3434516/1‑94 (MQ=60)
                                                                                     ACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCGGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAA         >  SRR4302543.2392254/1‑94 (MQ=60)
                                                                                     ACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAA         <  SRR4302543.907720/94‑1 (MQ=60)
                                                                                     ACCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAA         <  SRR4302543.7353307/94‑1 (MQ=60)
                                                                                      CCGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAA        <  SRR4302543.5010856/94‑1 (MQ=60)
                                                                                       CGTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAAT       >  SRR4302543.3896926/1‑94 (MQ=60)
                                                                                        GTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATA      >  SRR4302543.5188039/1‑94 (MQ=60)
                                                                                        GTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATA      >  SRR4302543.1487667/1‑94 (MQ=60)
                                                                                        GTCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATA      <  SRR4302543.4948653/94‑1 (MQ=60)
                                                                                         TCGGATGCCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATAC     <  SRR4302543.4567409/94‑1 (MQ=60)
                                                                                         TCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATAC     <  SRR4302543.375441/94‑1 (MQ=60)
                                                                                         TCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATAC     <  SRR4302543.375443/94‑1 (MQ=60)
                                                                                         TCGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATAC     <  SRR4302543.2421214/94‑1 (MQ=60)
                                                                                          CGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACA    <  SRR4302543.2907103/94‑1 (MQ=60)
                                                                                          CGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACA    >  SRR4302543.5428120/1‑94 (MQ=60)
                                                                                          CGCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACA    >  SRR4302543.7016124/1‑94 (MQ=60)
                                                                                           GCATGTCCATCGGTGAAGCGACAGCCCGCCGTGCGAAGAAAGAGATGGTGGAAGAGAACTTCCTCCTGGTTATTTCTTTCGCCAAGACACACAC   >  SRR4302543.1064154/1‑94 (MQ=44)
                                                                                           GCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACAC   >  SRR4302543.1064153/1‑94 (MQ=60)
                                                                                           GCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACAC   <  SRR4302543.1378559/94‑1 (MQ=60)
                                                                                           GCATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACAC   >  SRR4302543.1001048/1‑94 (MQ=60)
                                                                                            CATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  <  SRR4302543.1859192/94‑1 (MQ=60)
                                                                                            CATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  >  SRR4302543.6525843/1‑94 (MQ=60)
                                                                                            CATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  >  SRR4302543.6402060/1‑94 (MQ=60)
                                                                                            CATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  <  SRR4302543.6181113/94‑1 (MQ=60)
                                                                                            CATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  <  SRR4302543.3576181/94‑1 (MQ=60)
                                                                                            |                                                                                             
TCTGAAGAAGTGCATCGCGCCCTGCAAAAACTGCAGCAGATTGAAGAAGAAACCGGCCTGACCATCGAGCAGGTTAAAGATATCAACCGTCGTATGTCCATCGGTGAAGCGAAAGCCCGCCGTGCGAAGAAAGAGATGGTTGAAGCGAACTTACGTCTGGTTATTTCTATCGCTAAGAAATACACC  >  NC_000913/3211687‑3211872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑