Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,266,308 T→C S85P (TCT→CCT)  yhaC → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,266,3080TC99.4% 514.7 / ‑4.9 166S85P (TCT→CCT) yhaChypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (96/69);  total (97/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

Only 100 of 183 total aligned reads displayed.

ACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAA  >  NC_000913/3266218‑3266397
                                                                                          |                                                                                         
 cGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATgtctcgcctc                                                                                        <  2:915060/93‑1 (MQ=255)
  gTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCctct                                                                                       >  1:1422229/1‑93 (MQ=255)
  gTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCctct                                                                                       <  2:1182213/93‑1 (MQ=255)
     aCAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCg                                                                                    >  1:2837805/1‑93 (MQ=255)
      cAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGt                                                                                   >  1:1281490/1‑93 (MQ=255)
      cAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGt                                                                                   <  2:587258/93‑1 (MQ=255)
      cAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGt                                                                                   <  1:960626/93‑1 (MQ=255)
       aaCCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTc                                                                                  <  1:3463209/93‑1 (MQ=255)
       aaCCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTc                                                                                  >  1:1899912/1‑93 (MQ=255)
        aCCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCt                                                                                 >  1:3347116/1‑93 (MQ=255)
            ggtggAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGAt                                                                             >  2:3494565/1‑93 (MQ=255)
             gtggAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGAtt                                                                            <  2:3208060/93‑1 (MQ=255)
                  aaaTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTa                                                                       <  1:2362940/93‑1 (MQ=255)
                   aaTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAg                                                                      >  1:314949/1‑93 (MQ=255)
                   aaTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAg                                                                      <  1:514953/93‑1 (MQ=255)
                   aaTGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAg                                                                      >  2:3062537/1‑93 (MQ=255)
                    aTGCATTATCACTGGTGATTTTCAAAAAGAACTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAgg                                                                     >  2:2877133/1‑93 (MQ=255)
                      gCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGtt                                                                   <  2:960485/93‑1 (MQ=255)
                         ttATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTtata                                                                >  2:1935581/1‑93 (MQ=255)
                         ttATCACTGGGGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGGTTTTTTAGGTtata                                                                >  2:3834083/1‑93 (MQ=255)
                          tATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATaa                                                               <  1:2707100/93‑1 (MQ=255)
                          tATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATaa                                                               <  1:2856131/93‑1 (MQ=255)
                          tATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATaa                                                               <  2:429079/93‑1 (MQ=255)
                            tCACTGGTGATTTTCAAAAAGAATTAGCGGACTTACAAAATGCAATTCTTTATAATGTCTCGCCTCTTTGTCTTGATTTTTTAGTCTATAACg                                                             >  2:2990956/1‑93 (MQ=255)
                             cACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGc                                                            <  2:2241600/93‑1 (MQ=255)
                             cACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGc                                                            <  2:4313158/93‑1 (MQ=255)
                               cTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGccc                                                          <  2:4341697/93‑1 (MQ=255)
                                tGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGTGTATAACGCCCa                                                         >  1:4118036/1‑93 (MQ=255)
                                 ggTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCaa                                                        >  1:4489566/1‑93 (MQ=255)
                                   tgtttttCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAat                                                      >  2:653124/4‑93 (MQ=255)
                                    gATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAatt                                                     >  2:55790/1‑93 (MQ=255)
                                     aTTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAatta                                                    >  1:2008785/1‑93 (MQ=255)
                                      ttttCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAattat                                                   >  1:873123/1‑93 (MQ=255)
                                      ttttCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAattat                                                   >  1:715209/1‑93 (MQ=255)
                                       tttCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGCCTGGATTTTTTAGGTTATAACGCCCAAattatt                                                  >  2:327051/1‑93 (MQ=255)
                                          cAAAAAGAATTAGTGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCaa                                               <  1:3829123/93‑1 (MQ=255)
                                          cAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCaa                                               <  1:4208479/93‑1 (MQ=255)
                                          cAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCaa                                               <  1:4198493/93‑1 (MQ=255)
                                           aaaaaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAAc                                              >  2:1424028/1‑93 (MQ=255)
                                            aaaaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACg                                             >  1:553029/1‑93 (MQ=255)
                                             aaaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGa                                            >  1:4460479/1‑93 (MQ=255)
                                             aaaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGa                                            >  2:2696496/1‑93 (MQ=255)
                                              aaGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGAt                                           >  1:2919790/1‑93 (MQ=255)
                                               aGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATc                                          >  1:2609901/1‑93 (MQ=255)
                                               aGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATc                                          <  1:679583/93‑1 (MQ=255)
                                                gAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCg                                         <  2:1242592/93‑1 (MQ=255)
                                                gAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCg                                         <  1:2424132/93‑1 (MQ=255)
                                                gAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCg                                         >  1:304155/1‑93 (MQ=255)
                                                 aaTTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCgg                                        >  2:1151247/1‑93 (MQ=255)
                                                 aaTTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCgg                                        >  1:1064949/1‑93 (MQ=255)
                                                  aTTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGa                                       >  2:397150/1‑93 (MQ=255)
                                                  aTTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGa                                       >  2:780816/1‑93 (MQ=255)
                                                   ttAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGAc                                      <  2:186703/93‑1 (MQ=255)
                                                    tAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAGATTATTCAACGATCGGACa                                     <  2:4576873/93‑1 (MQ=255)
                                                    tAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACa                                     >  1:3710455/1‑93 (MQ=255)
                                                       cGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATa                                  <  1:1564437/93‑1 (MQ=255)
                                                        gTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATat                                 >  2:3202985/1‑92 (MQ=255)
                                                        gTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATAc                                 >  1:3253094/1‑93 (MQ=255)
                                                         tACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACt                                <  1:2934903/93‑1 (MQ=255)
                                                         tACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACt                                >  2:1541625/1‑93 (MQ=255)
                                                            ttACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTtgt                             <  2:134046/93‑1 (MQ=255)
                                                            ttACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTtgt                             >  1:1191245/1‑93 (MQ=255)
                                                            ttACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTtgt                             >  1:1770534/1‑93 (MQ=255)
                                                            ttACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTtgt                             <  2:4101149/93‑1 (MQ=255)
                                                            ttACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTtgt                             <  2:22038/93‑1 (MQ=255)
                                                              aCAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGa                           >  2:1462214/1‑93 (MQ=255)
                                                                aaaaTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAAc                         >  1:1584654/1‑93 (MQ=255)
                                                                aaaaTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAAc                         <  2:3111758/93‑1 (MQ=255)
                                                                aaaaTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAAc                         >  1:3322760/1‑93 (MQ=255)
                                                                aaaaTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAAc                         >  2:1118362/1‑93 (MQ=255)
                                                                aaaaTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGAGAAc                         >  2:4047002/1‑93 (MQ=255)
                                                                   aTGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTa                      >  1:2058575/1‑93 (MQ=255)
                                                                      tAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCa                   <  1:1362371/93‑1 (MQ=255)
                                                                       aaTTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCAt                  >  1:4032819/1‑93 (MQ=255)
                                                                       aaTTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCAt                  >  1:1434857/1‑93 (MQ=255)
                                                                        aTTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCAtt                 >  1:252733/1‑93 (MQ=255)
                                                                         ttCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTa                >  2:2305416/1‑93 (MQ=255)
                                                                          tCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTaa               <  1:478201/93‑1 (MQ=255)
                                                                          tCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTaa               <  1:721424/93‑1 (MQ=255)
                                                                           cTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAt              >  1:1645492/1‑93 (MQ=255)
                                                                           cTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAt              >  1:3405965/1‑93 (MQ=255)
                                                                            tttATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATg             >  1:4171169/1‑93 (MQ=255)
                                                                            tttATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATg             >  2:2895095/1‑93 (MQ=255)
                                                                             ttATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGa            <  1:581091/93‑1 (MQ=255)
                                                                             ttATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGa            >  1:3608826/1‑93 (MQ=255)
                                                                              tataATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGaa           >  1:3554681/1‑93 (MQ=255)
                                                                                taATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAAcc         <  2:3971192/93‑1 (MQ=255)
                                                                                taATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAAcc         >  1:2172674/1‑93 (MQ=255)
                                                                                 aaTGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCg        >  2:3162089/1‑93 (MQ=255)
                                                                                  aTGTCTCGCCTCTTCGTCTGAATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGt       <  1:3131995/93‑1 (MQ=255)
                                                                                   tGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGtt      <  2:2406115/93‑1 (MQ=255)
                                                                                   tGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGtt      >  2:498253/1‑93 (MQ=255)
                                                                                   tGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGtt      >  1:2531796/1‑93 (MQ=255)
                                                                                    gtctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTa     <  2:2662225/93‑1 (MQ=255)
                                                                                     tctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaa    <  2:4268206/93‑1 (MQ=255)
                                                                                     tctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaa    >  2:3451573/1‑93 (MQ=255)
                                                                                     tctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaa    <  1:1353421/93‑1 (MQ=255)
                                                                                      ctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaaa   >  2:4671153/1‑93 (MQ=255)
                                                                                      ctcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaaa   <  1:3699306/93‑1 (MQ=255)
                                                                                       tcgcctcTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTaaaa  <  2:4059206/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAA  >  NC_000913/3266218‑3266397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAAAACC  >  NC_000913/3266219‑3266401
                                                                                         |                                                                                             
CGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCT                                                                                           >  SRR4302535.644938/1‑94 (MQ=60)
CGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCT                                                                                           >  SRR4302535.1432384/1‑94 (MQ=60)
  TTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTC                                                                                         <  SRR4302535.2793050/94‑1 (MQ=60)
   TACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCG                                                                                        >  SRR4302535.2857940/1‑94 (MQ=60)
    ACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGT                                                                                       >  SRR4302535.1290636/1‑94 (MQ=60)
    ACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGT                                                                                       >  SRR4302535.3553145/1‑94 (MQ=60)
     CAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTC                                                                                      <  SRR4302535.967451/94‑1 (MQ=60)
     CAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTC                                                                                      >  SRR4302535.1913584/1‑94 (MQ=60)
      AACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCT                                                                                     <  SRR4302535.3487280/94‑1 (MQ=60)
      AACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCT                                                                                     >  SRR4302535.3370434/1‑94 (MQ=60)
       ACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTG                                                                                    >  SRR4302535.1403514/1‑94 (MQ=60)
        CCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGG                                                                                   >  SRR4302535.3439682/1‑94 (MQ=60)
         CTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGA                                                                                  >  SRR4302535.1750911/1‑94 (MQ=60)
                 AAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAG                                                                          >  SRR4302535.317126/1‑94 (MQ=60)
                 AAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAG                                                                          <  SRR4302535.2379911/94‑1 (MQ=60)
                  AATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGG                                                                         <  SRR4302535.3388523/94‑1 (MQ=60)
                  AATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGG                                                                         <  SRR4302535.518553/94‑1 (MQ=60)
                   ATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGT                                                                        >  SRR4302535.2994929/1‑94 (MQ=60)
                        TTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAA                                                                   <  SRR4302535.1579985/94‑1 (MQ=60)
                         TATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAAC                                                                  <  SRR4302535.2876369/94‑1 (MQ=60)
                         TATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAAC                                                                  <  SRR4302535.2726445/94‑1 (MQ=60)
                         TATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAAC                                                                  <  SRR4302535.4334873/94‑1 (MQ=60)
                             ACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCC                                                              >  SRR4302535.725228/1‑94 (MQ=60)
                              CTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGTGTATAACGCCCA                                                             >  SRR4302535.4146221/1‑94 (MQ=60)
                               TGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAA                                                            >  SRR4302535.4520383/1‑94 (MQ=60)
                                  TGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATT                                                         >  SRR4302535.1317160/1‑94 (MQ=60)
                                  TGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATT                                                         <  SRR4302535.2910168/94‑1 (MQ=60)
                                   GATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTA                                                        >  SRR4302535.2023252/1‑94 (MQ=60)
                                    ATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTAT                                                       >  SRR4302535.879312/1‑94 (MQ=60)
                                    ATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTAT                                                       >  SRR4302535.2626025/1‑94 (MQ=60)
                                    ATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTAT                                                       >  SRR4302535.720228/1‑94 (MQ=60)
                                       TTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCA                                                    >  SRR4302535.4215557/1‑94 (MQ=60)
                                         CAAAAAGAATTAGTGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAAC                                                  <  SRR4302535.3855533/94‑1 (MQ=60)
                                         CAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAAC                                                  <  SRR4302535.4237283/94‑1 (MQ=60)
                                         CAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAAC                                                  <  SRR4302535.2862796/94‑1 (MQ=60)
                                         CAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAAC                                                  <  SRR4302535.4227224/94‑1 (MQ=60)
                                          AAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACG                                                 >  SRR4302535.2034160/1‑94 (MQ=60)
                                          AAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACG                                                 >  SRR4302535.556900/1‑94 (MQ=60)
                                           AAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGA                                                >  SRR4302535.4491072/1‑94 (MQ=60)
                                            AAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGAT                                               <  SRR4302535.1368473/94‑1 (MQ=60)
                                            AAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGAT                                               >  SRR4302535.2940391/1‑94 (MQ=60)
                                             AAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATC                                              >  SRR4302535.2628629/1‑94 (MQ=60)
                                              AGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCG                                             >  SRR4302535.306267/1‑94 (MQ=60)
                                              AGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCG                                             <  SRR4302535.684343/94‑1 (MQ=60)
                                              AGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCG                                             >  SRR4302535.4036342/1‑94 (MQ=60)
                                               GAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGG                                            >  SRR4302535.1072526/1‑94 (MQ=60)
                                               GAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGG                                            <  SRR4302535.2441598/94‑1 (MQ=60)
                                                AATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGA                                           >  SRR4302535.2408021/1‑94 (MQ=60)
                                                  TTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACA                                         >  SRR4302535.3736156/1‑94 (MQ=60)
                                                    AGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAAT                                       <  SRR4302535.4140302/94‑1 (MQ=60)
                                                    AGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAAT                                       >  SRR4302535.545373/1‑94 (MQ=60)
                                                      CGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATAC                                     <  SRR4302535.1575731/94‑1 (MQ=60)
                                                      CGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATAC                                     >  SRR4302535.3161581/1‑94 (MQ=60)
                                                      CGTACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATAC                                     >  SRR4302535.3275696/1‑94 (MQ=60)
                                                        TACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTT                                   <  SRR4302535.1229716/94‑1 (MQ=60)
                                                        TACTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTT                                   <  SRR4302535.2955558/94‑1 (MQ=60)
                                                          CTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGT                                 >  SRR4302535.4148631/1‑94 (MQ=60)
                                                          CTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGT                                 >  SRR4302535.1199774/1‑94 (MQ=60)
                                                          CTTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGT                                 >  SRR4302535.1783366/1‑94 (MQ=60)
                                                           TTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTG                                <  SRR4302535.3195618/94‑1 (MQ=60)
                                                           TTACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTG                                <  SRR4302535.3761830/94‑1 (MQ=60)
                                                            TACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGA                               >  SRR4302535.1625752/1‑94 (MQ=60)
                                                             ACAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAA                              <  SRR4302535.779605/94‑1 (MQ=60)
                                                              CAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAAC                             >  SRR4302535.1865473/1‑94 (MQ=60)
                                                              CAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAAC                             >  SRR4302535.3345879/1‑94 (MQ=60)
                                                              CAAAATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAAC                             >  SRR4302535.1596086/1‑94 (MQ=60)
                                                                 AATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTA                          >  SRR4302535.2073405/1‑94 (MQ=60)
                                                                  ATGTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATCACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTAC                         <  SRR4302535.2534840/94‑1 (MQ=60)
                                                                    GTAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCA                       >  SRR4302535.1463472/1‑94 (MQ=60)
                                                                     TAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCAT                      <  SRR4302535.1372115/94‑1 (MQ=60)
                                                                     TAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCAT                      >  SRR4302535.4060407/1‑94 (MQ=60)
                                                                     TAATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCAT                      >  SRR4302535.1445105/1‑94 (MQ=60)
                                                                      AATTCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATT                     >  SRR4302535.254475/1‑94 (MQ=60)
                                                                         TCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAT                  <  SRR4302535.726488/94‑1 (MQ=60)
                                                                         TCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAT                  >  SRR4302535.3429668/1‑94 (MQ=60)
                                                                         TCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAT                  >  SRR4302535.1657383/1‑94 (MQ=60)
                                                                         TCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAT                  <  SRR4302535.481555/94‑1 (MQ=60)
                                                                         TCTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAAT                  <  SRR4302535.4492293/94‑1 (MQ=60)
                                                                          CTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATG                 >  SRR4302535.4199727/1‑94 (MQ=60)
                                                                          CTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATG                 <  SRR4302535.702266/94‑1 (MQ=60)
                                                                          CTTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATG                 <  SRR4302535.2310444/94‑1 (MQ=60)
                                                                           TTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGA                >  SRR4302535.3633853/1‑94 (MQ=60)
                                                                           TTTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGA                <  SRR4302535.3088031/94‑1 (MQ=60)
                                                                            TTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAA               >  SRR4302535.3579339/1‑94 (MQ=60)
                                                                            TTATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAA               <  SRR4302535.585174/94‑1 (MQ=60)
                                                                             TATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAAC              >  SRR4302535.3693862/1‑94 (MQ=60)
                                                                              ATAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACC             >  SRR4302535.2188219/1‑94 (MQ=60)
                                                                               TAATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCG            <  SRR4302535.3305226/94‑1 (MQ=60)
                                                                                AATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGT           >  SRR4302535.3624189/1‑94 (MQ=60)
                                                                                 ATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTT          >  SRR4302535.2628835/1‑94 (MQ=60)
                                                                                 ATGTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTT          >  SRR4302535.2550038/1‑94 (MQ=60)
                                                                                 ATGTCTCGCCTCTTCGTCTGAATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTT          <  SRR4302535.3153807/94‑1 (MQ=60)
                                                                                   GTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAA        <  SRR4302535.517772/94‑1 (MQ=60)
                                                                                   GTCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAA        >  SRR4302535.4600923/1‑94 (MQ=60)
                                                                                    TCTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAA       <  SRR4302535.1363105/94‑1 (MQ=60)
                                                                                     CTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAA      <  SRR4302535.2151024/94‑1 (MQ=60)
                                                                                     CTCGCCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAA      <  SRR4302535.3724930/94‑1 (MQ=60)
                                                                                         CCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAAAACC  <  SRR4302535.4120261/94‑1 (MQ=60)
                                                                                         CCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAAAACC  >  SRR4302535.445448/1‑94 (MQ=60)
                                                                                         |                                                                                             
CGTTACAACCTGGTGGAAAATGCATTATCACTGGTGATTTTCAAAAAGAATTAGCGTACTTACAAAATGTAATTCTTTATAATGTCTCGTCTCTTCGTCTGGATTTTTTAGGTTATAACGCCCAAATTATTCAACGATCGGACAATACTTGTGAACTTACCATTAATGAACCGTTAAAAAACC  >  NC_000913/3266219‑3266401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑