Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,273,491 T→C V190A (GTA→GCA)  yhaG → putative hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,273,4910TC95.5% 517.6 / 7.7 178V190A (GTA→GCA) yhaGputative hydrolase
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base C (65/105);  total (69/109)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

Only 100 of 188 total aligned reads displayed.

TGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCT  >  NC_000913/3273401‑3273582
                                                                                          |                                                                                           
tGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAc                                                                                           >  1:4175688/1‑93 (MQ=255)
tGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAc                                                                                           >  2:4162588/1‑93 (MQ=255)
tGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAc                                                                                           >  1:779922/1‑93 (MQ=255)
 gCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAcc                                                                                          <  2:2097351/93‑1 (MQ=255)
  cTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCt                                                                                         <  2:1883634/93‑1 (MQ=255)
     ccGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATc                                                                                      <  1:204203/93‑1 (MQ=255)
     ccGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATc                                                                                      >  1:1653739/1‑93 (MQ=255)
      cGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATcc                                                                                     <  1:896255/93‑1 (MQ=255)
          aTACCCGAACGTCGATGGCGTGGTGGGTCTGAATCATTTGTACGGTTGTGGCGTGGCTATTAACGCACCGGCGGCAGTTGCACCTATCCGTAc                                                                                 <  1:3273006/93‑1 (MQ=255)
             cccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCCGTTGCACCTATCCGTCCCAt                                                                              >  1:1308145/1‑93 (MQ=255)
              ccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGGAGTTGCACCTATCCGTACCCtt                                                                             >  2:897598/1‑93 (MQ=255)
              ccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCAtt                                                                             >  1:1926231/1‑93 (MQ=255)
              ccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCAtt                                                                             >  2:3699701/1‑93 (MQ=255)
              ccGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCAtt                                                                             <  1:644900/93‑1 (MQ=255)
               cGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTc                                                                            <  2:117383/93‑1 (MQ=255)
               cGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTc                                                                            >  2:2332142/1‑93 (MQ=255)
               cGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTc                                                                            >  2:1450780/1‑93 (MQ=255)
                gAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTca                                                                           <  2:2022057/93‑1 (MQ=255)
                 aaCGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTcac                                                                          <  1:112137/93‑1 (MQ=255)
                  aCGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTcaca                                                                         <  1:211712/93‑1 (MQ=255)
                  aCGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTcaca                                                                         <  2:1759645/93‑1 (MQ=255)
                   cGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACaa                                                                        <  1:4638708/93‑1 (MQ=255)
                    gTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCTATTAACGCACCGGCGGCAGTTGTCCCTATCCGTACCATTCACAat                                                                       <  2:2752570/93‑1 (MQ=255)
                      cGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAatat                                                                     <  2:4481158/93‑1 (MQ=255)
                       gATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATAtt                                                                    >  2:2800266/1‑93 (MQ=255)
                       gATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATAtt                                                                    <  1:933886/93‑1 (MQ=255)
                        aTGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATAttt                                                                   >  1:1732863/1‑93 (MQ=255)
                        aTGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGGGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATAttt                                                                   >  2:1865042/1‑93 (MQ=255)
                         tGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTc                                                                  <  1:896997/93‑1 (MQ=255)
                         tGGCGTGGGGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAAAGCACCGGCGGCAGTTGCACCTATCCGGACCATTCACAATATTTc                                                                  >  2:2494633/1‑93 (MQ=255)
                          ggCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCg                                                                 >  1:1636021/1‑93 (MQ=255)
                           gCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGc                                                                <  1:4246421/93‑1 (MQ=255)
                            cGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCt                                                               >  1:983358/1‑93 (MQ=255)
                               gctggGGCTGAATCATTTGTACGGTTGTGGCGTGGCAATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGaa                                                            <  1:2478742/91‑1 (MQ=255)
                                gtggGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAAt                                                           <  2:4388565/93‑1 (MQ=255)
                                 tggGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATc                                                          >  2:2393278/1‑93 (MQ=255)
                                 tggGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATc                                                          <  2:1431219/93‑1 (MQ=255)
                                  ggggCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATcc                                                         >  1:2800792/1‑93 (MQ=255)
                                  ggggCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATcc                                                         <  1:151888/93‑1 (MQ=255)
                                  ggggCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATcc                                                         <  1:2921579/93‑1 (MQ=255)
                                  ggggCTGAATCACTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATcc                                                         <  1:1983922/93‑1 (MQ=255)
                                       tGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGCATCCTAACt                                                    >  2:4344410/1‑93 (MQ=255)
                                        taaTCGTTTGTACGGTTGTGGCGTGGCGATTAACGGTCCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACtt                                                   <  1:1154768/92‑1 (MQ=255)
                                        gAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACtt                                                   >  2:1709139/1‑93 (MQ=255)
                                        gAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACtt                                                   <  2:3344855/93‑1 (MQ=255)
                                        gAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACtt                                                   <  1:2208216/93‑1 (MQ=255)
                                         aaTCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACttt                                                  <  1:3360911/93‑1 (MQ=255)
                                          aTCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTg                                                 <  2:3467599/93‑1 (MQ=255)
                                            cATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggc                                               <  2:4128106/93‑1 (MQ=255)
                                            cATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggc                                               <  2:3831152/93‑1 (MQ=255)
                                             aTTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcg                                              >  2:2120561/1‑93 (MQ=255)
                                             aTTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcg                                              >  1:3503658/1‑93 (MQ=255)
                                             aTTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcg                                              >  2:1072230/1‑93 (MQ=255)
                                              tttGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcgg                                             >  2:3126759/1‑93 (MQ=255)
                                              tttGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcgg                                             >  1:3569396/1‑93 (MQ=255)
                                               ttGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTggcggc                                            <  2:3390346/93‑1 (MQ=255)
                                                  tACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGaa                                         <  2:70464/93‑1 (MQ=255)
                                                   aCGGTTGTGGCGTGTCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAg                                        <  2:2696873/93‑1 (MQ=255)
                                                   aCGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAg                                        <  2:1161970/93‑1 (MQ=255)
                                                    cGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGt                                       <  1:2335245/93‑1 (MQ=255)
                                                    cGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGt                                       <  1:3466183/93‑1 (MQ=255)
                                                      gTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTaa                                     <  2:3147111/93‑1 (MQ=255)
                                                       ttGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAAt                                    <  1:49005/93‑1 (MQ=255)
                                                       ttGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAAt                                    <  1:578823/93‑1 (MQ=255)
                                                       ttGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAAt                                    <  1:2394382/93‑1 (MQ=255)
                                                       ttGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAAt                                    <  1:3411491/93‑1 (MQ=255)
                                                          tggcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGt                                 >  1:3822403/1‑93 (MQ=255)
                                                          tggcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGt                                 <  1:1105986/93‑1 (MQ=255)
                                                           ggcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTg                                <  2:2205398/93‑1 (MQ=255)
                                                            gcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCTAAGTAATGGTGa                               <  2:1557906/93‑1 (MQ=255)
                                                            gcgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGa                               >  1:2993261/1‑93 (MQ=255)
                                                             cgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGAt                              <  1:13975/93‑1 (MQ=255)
                                                             cgtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGAt                              <  2:3485517/93‑1 (MQ=255)
                                                              gtggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGAtt                             <  1:1765974/93‑1 (MQ=255)
                                                               tggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTg                            >  2:4374402/1‑93 (MQ=255)
                                                               tggcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTg                            <  1:3744936/93‑1 (MQ=255)
                                                                 gcgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGc                          <  2:2321559/93‑1 (MQ=255)
                                                                  cgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGcc                         <  2:1901467/93‑1 (MQ=255)
                                                                  cgATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGcc                         <  2:3600401/93‑1 (MQ=255)
                                                                   gATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCt                        <  2:3247893/93‑1 (MQ=255)
                                                                   gATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCt                        >  1:3601364/1‑93 (MQ=255)
                                                                     ttAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTgg                      >  2:2027048/1‑93 (MQ=255)
                                                                     ttAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTgg                      <  2:1212914/93‑1 (MQ=255)
                                                                     ttAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTgg                      <  1:3134475/93‑1 (MQ=255)
                                                                       aaCGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGt                    <  2:4016740/93‑1 (MQ=255)
                                                                        aCGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGtt                   <  2:612165/93‑1 (MQ=255)
                                                                          gCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTtgt                 <  2:3792658/93‑1 (MQ=255)
                                                                          gCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTtgt                 <  1:3984665/93‑1 (MQ=255)
                                                                          gCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTtgt                 >  1:4277602/1‑93 (MQ=255)
                                                                            aCCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGa               >  2:523918/1‑93 (MQ=255)
                                                                             ccGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGaa              >  1:3923234/1‑93 (MQ=255)
                                                                               ggcggcAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGaaaa            >  2:2172413/1‑93 (MQ=255)
                                                                                 cggcAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGt          <  2:2845107/93‑1 (MQ=255)
                                                                                  ggcAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGtt         <  2:3642005/93‑1 (MQ=255)
                                                                                   gcAGTTGCACCTATCCGTACCATTCACAATATTTCGCTCAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGGTg        >  2:1524422/1‑93 (MQ=255)
                                                                                      gTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAg     >  2:2443018/1‑93 (MQ=255)
                                                                                      gTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAg     >  2:815336/1‑93 (MQ=255)
                                                                                      gTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGATGCAg     >  2:3319516/1‑93 (MQ=255)
                                                                                       ttGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGc    >  2:3250303/1‑93 (MQ=255)
                                                                                         gTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCt  >  2:4242384/1‑93 (MQ=255)
                                                                                          |                                                                                           
TGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCT  >  NC_000913/3273401‑3273582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGA  >  NC_000913/3273400‑3273584
                                                                                           |                                                                                             
CTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAC                                                                                             >  SRR4302535.785396/1‑94 (MQ=60)
CTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAC                                                                                             >  SRR4302535.4204269/1‑94 (MQ=60)
CTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCAC                                                                                             <  SRR4302535.2258587/94‑1 (MQ=60)
 TGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACC                                                                                            >  SRR4302535.2083211/1‑94 (MQ=60)
   CTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTA                                                                                          >  SRR4302535.715327/1‑94 (MQ=60)
    TACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTAT                                                                                         <  SRR4302535.4090134/94‑1 (MQ=60)
     ACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATC                                                                                        >  SRR4302535.1665706/1‑94 (MQ=60)
      CCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCC                                                                                       <  SRR4302535.205602/94‑1 (MQ=60)
      CCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCC                                                                                       >  SRR4302535.3909608/1‑94 (MQ=60)
       CGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCG                                                                                      <  SRR4302535.902610/94‑1 (MQ=60)
           ATACCCGAACGTCGATGGCGTGGTGGGTCTGAATCATTTGTACGGTTGTGGCGTGGCTATTAACGCACCGGCGGCAGTTGCACCTATCCGTACC                                                                                  <  SRR4302535.3295740/94‑1 (MQ=60)
             ACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCCGTTGCACCTATCCGTCCCAT                                                                                >  SRR4302535.1317488/1‑94 (MQ=60)
              CCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATT                                                                               >  SRR4302535.1940114/1‑94 (MQ=60)
               CCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTC                                                                              >  SRR4302535.1141000/1‑94 (MQ=60)
               CCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTC                                                                              <  SRR4302535.649400/94‑1 (MQ=60)
                 GAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCAC                                                                            <  SRR4302535.2850624/94‑1 (MQ=60)
                  AACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACA                                                                           <  SRR4302535.112925/94‑1 (MQ=60)
                   ACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAA                                                                          >  SRR4302535.2383133/1‑94 (MQ=60)
                   ACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAA                                                                          <  SRR4302535.213167/94‑1 (MQ=60)
                    CGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAAT                                                                         <  SRR4302535.4670168/94‑1 (MQ=60)
                    CGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAAT                                                                         >  SRR4302535.15356/1‑94 (MQ=60)
                     GTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATA                                                                        >  SRR4302535.3606431/1‑94 (MQ=60)
                     GTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATA                                                                        <  SRR4302535.3338171/94‑1 (MQ=60)
                        GATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTT                                                                     >  SRR4302535.1745387/1‑94 (MQ=60)
                        GATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTT                                                                     >  SRR4302535.198872/1‑94 (MQ=60)
                        GATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTT                                                                     <  SRR4302535.940495/94‑1 (MQ=60)
                         ATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTT                                                                    >  SRR4302535.3481612/1‑94 (MQ=60)
                          TGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCG                                                                   >  SRR4302535.1647844/1‑94 (MQ=60)
                          TGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCG                                                                   <  SRR4302535.903356/94‑1 (MQ=60)
                          TGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCG                                                                   <  SRR4302535.942272/94‑1 (MQ=60)
                            GCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCT                                                                 >  SRR4302535.990342/1‑94 (MQ=60)
                            GCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCT                                                                 <  SRR4302535.3580071/94‑1 (MQ=60)
                            GCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCT                                                                 <  SRR4302535.4397142/94‑1 (MQ=60)
                            GCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCT                                                                 <  SRR4302535.4275495/94‑1 (MQ=60)
                             CGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTG                                                                >  SRR4302535.3430908/1‑94 (MQ=60)
                                GGTGGGTCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAAT                                                             <  SRR4302535.895405/94‑1 (MQ=60)
                                GCTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCAATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAAT                                                             <  SRR4302535.2496622/94‑1 (MQ=60)
                                  TGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCC                                                           >  SRR4302535.2820714/1‑94 (MQ=60)
                                   GGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCT                                                          <  SRR4302535.2942189/94‑1 (MQ=60)
                                   GGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCT                                                          <  SRR4302535.152950/94‑1 (MQ=60)
                                   GGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCT                                                          <  SRR4302535.2249816/94‑1 (MQ=60)
                                   GGGGCTGAATCACTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCT                                                          <  SRR4302535.1998204/94‑1 (MQ=60)
                                    GGGTTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTA                                                         <  SRR4302535.3955818/94‑1 (MQ=60)
                                    GGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTA                                                         <  SRR4302535.512213/94‑1 (MQ=60)
                                      GCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAAC                                                       <  SRR4302535.636939/94‑1 (MQ=60)
                                        TGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTT                                                     >  SRR4302535.1330698/1‑94 (MQ=60)
                                         TAATCGTTTGTACGGTTGTGGCGTGGCGATTAACGGTCCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTT                                                    <  SRR4302535.1163023/94‑1 (MQ=60)
                                         GAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTT                                                    >  SRR4302535.1711621/1‑94 (MQ=60)
                                         GAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTT                                                    <  SRR4302535.2223945/94‑1 (MQ=60)
                                         GAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTT                                                    <  SRR4302535.2020857/94‑1 (MQ=60)
                                          AATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTG                                                   <  SRR4302535.3384303/94‑1 (MQ=60)
                                             CATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCG                                                >  SRR4302535.3528009/1‑94 (MQ=60)
                                             CATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCG                                                >  SRR4302535.3008785/1‑94 (MQ=60)
                                              ATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGG                                               >  SRR4302535.3594150/1‑94 (MQ=60)
                                                  GTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAA                                           <  SRR4302535.916034/94‑1 (MQ=60)
                                                     CGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTA                                        <  SRR4302535.2351959/94‑1 (MQ=60)
                                                     CGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTA                                        <  SRR4302535.3490268/94‑1 (MQ=60)
                                                        TTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATG                                     <  SRR4302535.582891/94‑1 (MQ=60)
                                                        TTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATG                                     <  SRR4302535.3435226/94‑1 (MQ=60)
                                                        TTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATG                                     <  SRR4302535.49375/94‑1 (MQ=60)
                                                        TTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATG                                     <  SRR4302535.2411592/94‑1 (MQ=60)
                                                          GTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGT                                   >  SRR4302535.3848770/1‑94 (MQ=60)
                                                          GTGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGG                                   >  SRR4302535.118886/1‑94 (MQ=60)
                                                           TGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTG                                  <  SRR4302535.1113887/94‑1 (MQ=60)
                                                           TGGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTG                                  <  SRR4302535.3022132/94‑1 (MQ=60)
                                                            GGCGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGA                                 >  SRR4302535.3014250/1‑94 (MQ=60)
                                                              CGTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATT                               <  SRR4302535.14076/94‑1 (MQ=60)
                                                               GTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTG                              <  SRR4302535.2568381/94‑1 (MQ=60)
                                                               GTGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTG                              <  SRR4302535.1778777/94‑1 (MQ=60)
                                                                TGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGG                             <  SRR4302535.3770846/94‑1 (MQ=60)
                                                                TGGCGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGG                             >  SRR4302535.149708/1‑94 (MQ=60)
                                                                   CGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCT                          >  SRR4302535.1654106/1‑94 (MQ=60)
                                                                   CGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCT                          <  SRR4302535.2147591/94‑1 (MQ=60)
                                                                   CGATTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCT                          >  SRR4302535.3626354/1‑94 (MQ=60)
                                                                      TTAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGG                       <  SRR4302535.3156297/94‑1 (MQ=60)
                                                                       TAACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGT                      <  SRR4302535.4678348/94‑1 (MQ=60)
                                                                         ACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTG                    <  SRR4302535.1782561/94‑1 (MQ=60)
                                                                         ACGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTG                    >  SRR4302535.3276863/1‑94 (MQ=60)
                                                                          CGCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGT                   >  SRR4302535.4306878/1‑94 (MQ=60)
                                                                           GCACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTG                  <  SRR4302535.4011890/94‑1 (MQ=60)
                                                                             ACCGGCGGCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAA                >  SRR4302535.3950046/1‑94 (MQ=60)
                                                                                    GCAGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGC         <  SRR4302535.4294775/94‑1 (MQ=60)
                                                                                      AGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAG       >  SRR4302535.3735124/1‑94 (MQ=60)
                                                                                      AGTTGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACCTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGCTGCAC       >  SRR4302535.3826560/1‑94 (MQ=60)
                                                                                         TGCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCT    <  SRR4302535.1491235/94‑1 (MQ=60)
                                                                                          GCACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTG   >  SRR4302535.3761833/1‑94 (MQ=60)
                                                                                           CACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGA  >  SRR4302535.1604652/1‑94 (MQ=60)
                                                                                           |                                                                                             
CTGCTACCGAAATACCCGAACGTCGATGGCGTGGTGGGGCTGAATCATTTGTACGGTTGTGGCGTGGCGATTAACGCACCGGCGGCAGTTGTACCTATCCGTACCATTCACAATATTTCGCTGAATCCTAACTTTGGCGGCGAAGTAATGGTGATTGGCCTGGGTTGTGAAAAGTTGCAGCCTGA  >  NC_000913/3273400‑3273584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑