Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,339,422	T→C	D172D (GAT→GAC)	yrbH →	putative isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,339,422	0	T	C	100.0%	737.3 / NA	236	D172D (GAT→GAC)	yrbH	putative isomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (122/114); total (122/114)

Only 100 of 245 total aligned reads displayed.

TGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACT  >  NC_000913/3339332‑3339511

 gtgCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGcc                                                                                        >  2:1078410/1‑93 (MQ=255)

  tgCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGccc                                                                                       <  1:799286/93‑1 (MQ=255)

  tgCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGAGACGccc                                                                                       >  1:2410845/1‑93 (MQ=255)

   gCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGATCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCt                                                                                      <  1:3385055/93‑1 (MQ=255)

    cATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTc                                                                                     >  2:263000/1‑93 (MQ=255)

    cATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTc                                                                                     >  2:2525261/1‑93 (MQ=255)

     aTCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCg                                                                                    >  1:3973408/1‑93 (MQ=255)

      tCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGc                                                                                   >  1:743945/1‑93 (MQ=255)

      tCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGc                                                                                   <  1:3289387/93‑1 (MQ=255)

        tgtgtgTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTg                                                                                 >  2:961141/1‑93 (MQ=255)

        tgtgtgTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTg                                                                                 >  2:2934389/1‑93 (MQ=255)

         gtgtgtTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGt                                                                                >  2:4361511/1‑93 (MQ=255)

         gtgtgtTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGt                                                                                <  1:4472429/93‑1 (MQ=255)

          tgtgtTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTc                                                                               >  1:1984204/1‑93 (MQ=255)

          tgtgtTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGATGTc                                                                               >  2:4059386/1‑93 (MQ=255)

            tgtTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTcgc                                                                             >  1:3853891/1‑93 (MQ=255)

            tgtTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTcgc                                                                             >  1:838656/1‑93 (MQ=255)

              ttAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCgcgc                                                                           >  2:946923/1‑93 (MQ=255)

              ttAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCgcgc                                                                           >  2:616480/1‑93 (MQ=255)

                 aaGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGt                                                                        >  2:1818757/1‑93 (MQ=255)

                 aaGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGt                                                                        >  2:1173468/1‑93 (MQ=255)

                  aGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGtt                                                                       <  1:205248/93‑1 (MQ=255)

                  aGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGtt                                                                       <  2:1499149/93‑1 (MQ=255)

                   gTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTa                                                                      <  1:3133973/93‑1 (MQ=255)

                   gTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTa                                                                      >  2:3657137/1‑93 (MQ=255)

                    tAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTaa                                                                     >  1:361894/1‑93 (MQ=255)

                    tAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTaa                                                                     <  1:2187759/93‑1 (MQ=255)

                     aGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTaaa                                                                    <  1:912975/93‑1 (MQ=255)

                      gCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTaaaa                                                                   >  2:2260864/1‑93 (MQ=255)

                       cGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAg                                                                  >  1:2601793/1‑93 (MQ=255)

                        gAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGc                                                                 <  2:2853029/93‑1 (MQ=255)

                         aaaGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCa                                                                <  2:4216874/93‑1 (MQ=255)

                          aagaagCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCAc                                                               >  1:3653608/1‑93 (MQ=255)

                           agaagCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCAcg                                                              >  2:22698/1‑93 (MQ=255)

                            gaagCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCAcgc                                                             <  1:1753476/93‑1 (MQ=255)

                            gaagCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCAcgc                                                             <  2:3253491/93‑1 (MQ=255)

                             aagCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCAcgcg                                                            <  2:4649374/93‑1 (MQ=255)

                                ccTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCt                                                         <  2:1270035/93‑1 (MQ=255)

                                 cTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCtt                                                        <  2:1869300/93‑1 (MQ=255)

                                 cTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCtt                                                        >  1:185893/1‑93 (MQ=255)

                                 cTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCtt                                                        >  1:167443/1‑93 (MQ=255)

                                  tGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCttt                                                       >  1:1039167/1‑93 (MQ=255)

                                  tGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCttt                                                       >  1:3170531/1‑93 (MQ=255)

                                  tGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGACGCGCTGTTAAAAGCACGCGGCttt                                                       >  1:4527953/1‑93 (MQ=255)

                                   gTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTa                                                      >  1:3551606/1‑93 (MQ=255)

                                   gTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTa                                                      >  1:3574878/1‑93 (MQ=255)

                                   gTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTa                                                      <  2:1662504/93‑1 (MQ=255)

                                    tCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTAc                                                     <  1:3117641/93‑1 (MQ=255)

                                    tCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTAc                                                     >  2:2083242/1‑93 (MQ=255)

                                    tCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTAc                                                     <  1:1620931/93‑1 (MQ=255)

                                     ccGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTAct                                                    <  2:4153204/93‑1 (MQ=255)

                                     ccGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTAct                                                    <  1:1483334/93‑1 (MQ=255)

                                       gTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTActgc                                                  <  2:1938192/93‑1 (MQ=255)

                                       gTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTActgc                                                  <  1:3254082/93‑1 (MQ=255)

                                       gTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCACTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTActgc                                                  >  1:2212642/1‑93 (MQ=255)

                                        ttAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTActgct                                                 >  2:4615245/1‑93 (MQ=255)

                                         tAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTActgctg                                                <  1:4049475/93‑1 (MQ=255)

                                          aGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTCATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGa                                               <  1:2352732/93‑1 (MQ=255)

                                            ggCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAg                                             >  2:2956368/1‑93 (MQ=255)

                                             gCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGa                                            <  2:4209699/93‑1 (MQ=255)

                                              cTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGAt                                           >  2:1114586/1‑93 (MQ=255)

                                               tGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGAtt                                          <  1:2796329/93‑1 (MQ=255)

                                                ggCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGAttt                                         <  2:3735919/93‑1 (MQ=255)

                                                 gCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGAtttt                                        <  1:1222692/93‑1 (MQ=255)

                                                 gCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGAtttt                                        >  1:4684045/1‑93 (MQ=255)

                                                  cACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTg                                       <  2:2321079/93‑1 (MQ=255)

                                                  cACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTg                                       <  2:4304142/93‑1 (MQ=255)

                                                   aCCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTgc                                      >  2:2906307/1‑93 (MQ=255)

                                                    ccGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTgcg                                     >  1:1376065/1‑93 (MQ=255)

                                                     cGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTgcgc                                    >  2:3495023/1‑93 (MQ=255)

                                                      gACCAGCAGCACCGCCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGct                                   <  1:1507021/93‑1 (MQ=255)

                                                      gACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGct                                   >  1:4308753/1‑93 (MQ=255)

                                                      gACCAGCAGCACCACCGCCACGCTGGTTATGGGAGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGct                                   >  1:3529763/1‑93 (MQ=255)

                                                       aCCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGctc                                  <  2:780852/93‑1 (MQ=255)

                                                        ccAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGctct                                 >  1:4208574/1‑93 (MQ=255)

                                                        ccAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGctct                                 <  1:749730/93‑1 (MQ=255)

                                                           gcagcaCCACCGCCGCGCGGGTTATGGGCGGCGCCCTCGCTGTCGCGCTGTTAAAATCACGCGGCTTTAGTGCTGAAGATTTTGCGCCCTcac                              <  2:3918463/93‑1 (MQ=255)

                                                           gcagcaCCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTcac                              >  2:2346519/1‑93 (MQ=255)

                                                            cagcaCCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTcaca                             <  2:2199919/93‑1 (MQ=255)

                                                              gcaCCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACAcc                           >  2:1797265/1‑93 (MQ=255)

                                                              gcaCCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACAcc                           >  1:1774551/1‑93 (MQ=255)

                                                                accaccGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCa                         >  2:67284/1‑93 (MQ=255)

                                                                 ccaccGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAg                        <  1:4505722/93‑1 (MQ=255)

                                                                  caccGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAgg                       >  2:1386171/1‑93 (MQ=255)

                                                                       ccACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGgcggcg                  >  1:637216/1‑93 (MQ=255)

                                                                         aCGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCa                >  2:121580/1‑93 (MQ=255)

                                                                          cGCTGGGTATGGGCGGCGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACGCCCAGGCGGCGCat               <  2:3213256/93‑2 (MQ=255)

                                                                           gCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACt              >  2:3536338/1‑93 (MQ=255)

                                                                            cTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTg             >  2:1022452/1‑93 (MQ=255)

                                                                             tGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTgg            <  2:753691/93‑1 (MQ=255)

                                                                             tGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTgg            >  2:358563/1‑93 (MQ=255)

                                                                              ggTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTggg           <  1:3016509/93‑1 (MQ=255)

                                                                               gTTATGGGCGCCGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGt          <  2:4014900/93‑1 (MQ=255)

                                                                               gTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGt          >  2:190512/1‑93 (MQ=255)

                                                                                ttATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTc         <  2:2458561/93‑1 (MQ=255)

                                                                                    gggCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTaa     >  2:4433796/1‑93 (MQ=255)

                                                                                    gggCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTaa     <  1:782224/93‑1 (MQ=255)

                                                                                    gggCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTaa     <  1:3324320/93‑1 (MQ=255)

                                                                                     ggCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTaaa    <  1:2296882/93‑1 (MQ=255)

                                                                                      gCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAAc   >  1:3738445/1‑93 (MQ=255)

TGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACT  >  NC_000913/3339332‑3339511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTG  >  NC_000913/3339329‑3339515

AGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGAC                                                                                               >  SRR4302535.758996/1‑94 (MQ=60)

AGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGAC                                                                                               >  SRR4302535.7491/1‑94 (MQ=60)

AGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGG‑TATGGGCGACG                                                                                              >  SRR4302535.458735/1‑94 (MQ=60)

 GATGTGCATCTGTGTGTTAAAGTAGCGATAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACG                                                                                              <  SRR4302535.547965/94‑1 (MQ=60)

 GATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACG                                                                                              >  SRR4302535.2492779/1‑94 (MQ=60)

  ATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGC                                                                                             >  SRR4302535.2214853/1‑94 (MQ=60)

    GTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCC                                                                                           >  SRR4302535.3690750/1‑94 (MQ=60)

    GTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGAGACGCCC                                                                                           >  SRR4302535.2428198/1‑94 (MQ=60)

     TGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCT                                                                                          <  SRR4302535.1469278/94‑1 (MQ=60)

     TGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCT                                                                                          <  SRR4302535.804898/94‑1 (MQ=60)

     TGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCT                                                                                          <  SRR4302535.283371/94‑1 (MQ=60)

      GCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGATCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTC                                                                                         <  SRR4302535.3408609/94‑1 (MQ=60)

      GCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTC                                                                                         >  SRR4302535.179790/1‑94 (MQ=60)

      GCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTC                                                                                         >  SRR4302535.509943/1‑94 (MQ=60)

       CATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCAGCGCCACGCTGGTTATGGGCGACGCCCTCG                                                                                        >  SRR4302535.4539393/1‑94 (MQ=60)

       CATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCG                                                                                        >  SRR4302535.4000549/1‑94 (MQ=60)

       CATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCG                                                                                        <  SRR4302535.3154006/94‑1 (MQ=60)

       CATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCG                                                                                        >  SRR4302535.2550399/1‑94 (MQ=60)

        ATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGC                                                                                       >  SRR4302535.749168/1‑94 (MQ=60)

         TCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCT                                                                                      <  SRR4302535.80476/94‑1 (MQ=60)

         TCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCT                                                                                      <  SRR4302535.3312257/94‑1 (MQ=60)

          CTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTG                                                                                     <  SRR4302535.1259817/94‑1 (MQ=60)

            GTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTC                                                                                   <  SRR4302535.4503124/94‑1 (MQ=60)

            GTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTC                                                                                   >  SRR4302535.3343153/1‑94 (MQ=60)

            GTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTC                                                                                   >  SRR4302535.1998490/1‑94 (MQ=60)

            GTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTC                                                                                   >  SRR4302535.3877387/1‑94 (MQ=60)

            GGGTGTTAAAGTAGCGAAAAAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTC                                                                                   <  SRR4302535.3619944/94‑1 (MQ=60)

              GTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGC                                                                                 >  SRR4302535.3880431/1‑94 (MQ=60)

              GTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGC                                                                                 >  SRR4302535.844545/1‑94 (MQ=60)

              GTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGC                                                                                 <  SRR4302535.1107339/94‑1 (MQ=60)

               TGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCG                                                                                >  SRR4302535.3474283/1‑94 (MQ=60)

                 TTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCT                                                                              <  SRR4302535.1807803/94‑1 (MQ=60)

                  TAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGTTGTCGCGCTG                                                                             >  SRR4302535.3181241/1‑94 (MQ=60)

                   AAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGT                                                                            <  SRR4302535.3382774/94‑1 (MQ=60)

                     AGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGCCCAGCAGCACCACCGCCACGCTGGATATGGGCGACGCCCTCGCTGTCGCGCTGTTA                                                                          >  SRR4302535.446427/1‑94 (MQ=60)

                     AGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTA                                                                          >  SRR4302535.4558593/1‑94 (MQ=60)

                     AGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTA                                                                          <  SRR4302535.206655/94‑1 (MQ=60)

                      GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAA                                                                         <  SRR4302535.3155793/94‑1 (MQ=60)

                      GTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAA                                                                         >  SRR4302535.364391/1‑94 (MQ=60)

                       TAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAA                                                                        <  SRR4302535.2203342/94‑1 (MQ=60)

                       GAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAA                                                                        <  SRR4302535.3739562/94‑1 (MQ=60)

                        AGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAA                                                                       <  SRR4302535.919463/94‑1 (MQ=60)

                        AGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAA                                                                       <  SRR4302535.2005292/94‑1 (MQ=60)

                         GCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAG                                                                      <  SRR4302535.3594777/94‑1 (MQ=60)

                         GCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAG                                                                      >  SRR4302535.2620472/1‑94 (MQ=60)

                         GCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAG                                                                      <  SRR4302535.1454483/94‑1 (MQ=60)

                           GAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCA                                                                    >  SRR4302535.746321/1‑94 (MQ=60)

                            AAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTACAACCAC                                                                   >  SRR4302535.154350/1‑94 (MQ=60)

                            AAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCAC                                                                   >  SRR4302535.3678905/1‑94 (MQ=60)

                             AAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACG                                                                  >  SRR4302535.4697218/1‑94 (MQ=60)

                             AAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACG                                                                  <  SRR4302535.3431734/94‑1 (MQ=60)

                             AAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACG                                                                  <  SRR4302535.1650183/94‑1 (MQ=60)

                               GAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCG                                                                <  SRR4302535.1766147/94‑1 (MQ=60)

                               GAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCG                                                                <  SRR4302535.2128419/94‑1 (MQ=60)

                                AAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGG                                                               <  SRR4302535.2356578/94‑1 (MQ=60)

                                 AGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGC                                                              >  SRR4302535.1000449/1‑94 (MQ=60)

                                   CCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTT                                                            >  SRR4302535.168606/1‑94 (MQ=60)

                                   CCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTT                                                            >  SRR4302535.187176/1‑94 (MQ=60)

                                   CCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTT                                                            >  SRR4302535.678819/1‑94 (MQ=60)

                                   CCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGCCGCGCTGTTAAAAGCACGCGGCTT                                                            >  SRR4302535.4533055/1‑94 (MQ=60)

                                    CTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTT                                                           >  SRR4302535.1046540/1‑94 (MQ=60)

                                    CTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTT                                                           >  SRR4302535.3192487/1‑94 (MQ=60)

                                    CTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTT                                                           >  SRR4302535.1580637/1‑94 (MQ=60)

                                    CTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGACGCGCTGTTAAAAGCACGCGGCTTT                                                           >  SRR4302535.4558963/1‑94 (MQ=60)

                                     TGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTA                                                          >  SRR4302535.3599664/1‑94 (MQ=60)

                                     TGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTA                                                          <  SRR4302535.1894943/94‑1 (MQ=60)

                                     TGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTA                                                          >  SRR4302535.3576252/1‑94 (MQ=60)

                                      GTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTAC                                                         >  SRR4302535.3583660/1‑94 (MQ=60)

                                       TCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACT                                                        <  SRR4302535.3139345/94‑1 (MQ=60)

                                       TCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACT                                                        <  SRR4302535.1632626/94‑1 (MQ=60)

                                       TCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACT                                                        <  SRR4302535.821552/94‑1 (MQ=60)

                                        CCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTG                                                       >  SRR4302535.986293/1‑94 (MQ=60)

                                        CCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTG                                                       <  SRR4302535.1493943/94‑1 (MQ=60)

                                         CGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCACTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGC                                                      >  SRR4302535.2228398/1‑94 (MQ=60)

                                          GTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCT                                                     >  SRR4302535.304755/1‑94 (MQ=60)

                                          GTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCT                                                     >  SRR4302535.2286462/1‑94 (MQ=60)

                                          GTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCT                                                     >  SRR4302535.835867/1‑94 (MQ=60)

                                          GTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCT                                                     <  SRR4302535.3276691/94‑1 (MQ=60)

                                           TTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTG                                                    >  SRR4302535.4196405/1‑94 (MQ=60)

                                            TAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGA                                                   >  SRR4302535.2801892/1‑94 (MQ=60)

                                            TAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGA                                                   <  SRR4302535.4077199/94‑1 (MQ=60)

                                             AGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTCATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAA                                                  <  SRR4302535.2369598/94‑1 (MQ=60)

                                              GGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAG                                                 <  SRR4302535.324392/94‑1 (MQ=60)

                                                  TGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTT                                             <  SRR4302535.2816230/94‑1 (MQ=60)

                                                   GGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTT                                            >  SRR4302535.4715727/1‑94 (MQ=60)

                                                    GCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTG                                           <  SRR4302535.784364/94‑1 (MQ=60)

                                                    GCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTG                                           <  SRR4302535.1231444/94‑1 (MQ=60)

                                                     CACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGC                                          <  SRR4302535.2169006/94‑1 (MQ=60)

                                                      ACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCG                                         >  SRR4302535.1385891/1‑94 (MQ=60)

                                                        CGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCT                                       >  SRR4302535.4338231/1‑94 (MQ=60)

                                                        CGACCAGCAGCACCACCGCCACGCTGGTTATGGGAGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCT                                       >  SRR4302535.3554272/1‑94 (MQ=60)

                                                         GACCAGCAGCACCGCCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTC                                      <  SRR4302535.1517857/94‑1 (MQ=60)

                                                         GACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTC                                      >  SRR4302535.4089481/1‑94 (MQ=60)

                                                         GACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTC                                      <  SRR4302535.1517856/94‑1 (MQ=60)

                                                          GCCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCT                                     <  SRR4302535.3559469/94‑1 (MQ=60)

                                                          ACCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCT                                     >  SRR4302535.4237378/1‑94 (MQ=60)

                                                           CCAGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTC                                    <  SRR4302535.755013/94‑1 (MQ=60)

                                                             AGCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCAC                                  <  SRR4302535.3892342/94‑1 (MQ=60)

                                                              GCAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACA                                 <  SRR4302535.4074772/94‑1 (MQ=60)

                                                               CAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACAC                                <  SRR4302535.171322/94‑1 (MQ=60)

                                                               CAGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACAC                                >  SRR4302535.753511/1‑94 (MQ=60)

                                                                AGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACC                               >  SRR4302535.878816/1‑94 (MQ=60)

                                                                AGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACC                               <  SRR4302535.75737/94‑1 (MQ=60)

                                                                AGCACCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACC                               >  SRR4302535.1787407/1‑94 (MQ=60)

                                                                AGCACCACCGCCACGCTGGTTATGGGCGACGACCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACC                               <  SRR4302535.2729378/94‑1 (MQ=60)

                                                                    CCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGG                           <  SRR4302535.36475/94‑1 (MQ=60)

                                                                    CCACCGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGG                           <  SRR4302535.4536653/94‑1 (MQ=60)

                                                                        CGCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGC                       >  SRR4302535.1704756/1‑94 (MQ=60)

                                                                         GCCACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCG                      >  SRR4302535.641664/1‑94 (MQ=60)

                                                                           CACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCA                    <  SRR4302535.2300227/94‑1 (MQ=60)

                                                                           CACGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCA                    >  SRR4302535.4430087/1‑94 (MQ=60)

                                                                             CGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACT                  <  SRR4302535.328008/94‑1 (MQ=60)

                                                                             CGCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACT                  <  SRR4302535.241209/94‑1 (MQ=60)

                                                                              GCTGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTG                 >  SRR4302535.3961672/1‑94 (MQ=60)

                                                                                TGGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGG               >  SRR4302535.2386761/1‑94 (MQ=60)

                                                                                 GGTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGT              <  SRR4302535.3037644/94‑1 (MQ=60)

                                                                                  GTTATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTC             <  SRR4302535.4310114/94‑1 (MQ=60)

                                                                                    TATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACATCCAGGCGGCGCACTGGGTCGT           >  SRR4302535.3238688/1‑94 (MQ=60)

                                                                                     ATGGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTA          <  SRR4302535.3126353/94‑1 (MQ=60)

                                                                                       GGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAA        <  SRR4302535.3347451/94‑1 (MQ=60)

                                                                                       GGGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAA        <  SRR4302535.787713/94‑1 (MQ=60)

                                                                                        GGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAAC       >  SRR4302535.3764321/1‑94 (MQ=60)

                                                                                        GGCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAAC       <  SRR4302535.2313321/94‑1 (MQ=60)

                                                                                         GCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGTCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACT      <  SRR4302535.1786215/94‑1 (MQ=60)

                                                                                         GCGACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACT      <  SRR4302535.333735/94‑1 (MQ=60)

                                                                                           GACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTC    >  SRR4302535.2985655/1‑94 (MQ=60)

                                                                                            ACGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCT   <  SRR4302535.3696447/94‑1 (MQ=60)

                                                                                             CGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTG  >  SRR4302535.4288576/1‑94 (MQ=60)

                                                                                             CGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTC  >  SRR4302535.694061/1‑94 (MQ=60)

AGATGTGCATCTGTGTGTTAAAGTAGCGAAAGAAGCCTGTCCGTTAGGGCTGGCACCGACCAGCAGCACCACCGCCACGCTGGTTATGGGCGATGCCCTCGCTGTCGCGCTGTTAAAAGCACGCGGCTTTACTGCTGAAGATTTTGCGCTCTCACACCCAGGCGGCGCACTGGGTCGTAAACTTCTG  >  NC_000913/3339329‑3339515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`