Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,353,950 A→G T562A (ACC→GCC)  gltB → glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,353,9500AG100.0% 616.9 / NA 197T562A (ACC→GCC) gltBglutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (96/101);  total (96/101)

Only 100 of 215 total aligned reads displayed.

ATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC  >  NC_000913/3353860‑3354039
                                                                                          |                                                                                         
   ttttACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGt                                                                                      <  1:1869035/92‑1 (MQ=255)
    tttACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTa                                                                                     >  1:1990010/1‑93 (MQ=255)
      tACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTAtg                                                                                   >  2:697135/1‑92 (MQ=255)
         gctcactCCCGCGAGCAGTTTGCCCAGGTCACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGt                                                                                <  2:1986310/89‑1 (MQ=255)
         gACTCCTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGt                                                                                <  1:972581/93‑1 (MQ=255)
         gACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGt                                                                                >  1:1894536/1‑93 (MQ=255)
          actactTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTc                                                                               >  2:3833631/1‑93 (MQ=255)
          actactTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTc                                                                               >  1:1627849/1‑93 (MQ=255)
            tactTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGt                                                                             >  1:4253849/1‑93 (MQ=255)
              ctTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGa                                                                           >  1:43516/1‑93 (MQ=255)
              ctTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGa                                                                           >  1:3859038/1‑93 (MQ=255)
               ttCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGaa                                                                          >  2:578719/1‑93 (MQ=255)
               ttCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCCGTCGTGaa                                                                          >  2:105430/1‑93 (MQ=255)
               tcccGCCAGCATTTTGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGaa                                                                          <  2:848758/91‑1 (MQ=255)
                tCCGCCAGCAGTTTGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGaaa                                                                         <  2:282669/93‑1 (MQ=255)
                    ccAGCAGTTTGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGaa                                                                     <  2:1766931/93‑1 (MQ=255)
                    ccAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGaa                                                                     >  2:1486093/1‑93 (MQ=255)
                    ccAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGaa                                                                     >  1:3349087/1‑93 (MQ=255)
                    ccAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGaa                                                                     >  2:1566670/1‑93 (MQ=255)
                      agcagTTTGCCCGGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACg                                                                   <  2:4290734/93‑1 (MQ=255)
                       gcatTTTGCCCAGCTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGt                                                                  <  1:2464126/93‑1 (MQ=255)
                        cagTTTGCCCGGGGGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTc                                                                 <  1:3585467/93‑1 (MQ=255)
                          gTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGACATGAACGTCtt                                                               >  1:752645/1‑93 (MQ=255)
                          gTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCtt                                                               >  2:2199402/1‑93 (MQ=255)
                          gTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCtt                                                               >  1:490809/1‑93 (MQ=255)
                          gTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCtt                                                               >  2:2649073/1‑93 (MQ=255)
                            ttGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCtttt                                                             <  1:1210274/93‑1 (MQ=255)
                            tcgCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCtttt                                                             <  2:4551650/91‑1 (MQ=255)
                              gCCCAGTTCACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGc                                                           <  1:2431742/93‑1 (MQ=255)
                              cccgaGGTTACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGc                                                           <  2:783828/89‑1 (MQ=255)
                                ccAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGa                                                         >  2:3599203/1‑93 (MQ=255)
                                 cAGTTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGaa                                                        <  1:3966133/93‑1 (MQ=255)
                                 cAGTTGACTAACCCGCCAATCGCCCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGaa                                                        <  1:428457/93‑1 (MQ=255)
                                   ggTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGc                                                      >  1:1372994/1‑93 (MQ=255)
                                   ggTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGc                                                      >  2:3158395/1‑93 (MQ=255)
                                    gtaaCTACCCCGCCAAGCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCa                                                     <  1:2130895/90‑1 (MQ=255)
                                    gTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCa                                                     >  2:619233/1‑93 (MQ=255)
                                      gACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCaga                                                   <  2:2991571/93‑1 (MQ=255)
                                      gACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCagg                                                   >  1:1003683/1‑92 (MQ=255)
                                        cTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAgg                                                 <  1:2377421/93‑1 (MQ=255)
                                         taccccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAggg                                                <  1:3818662/90‑1 (MQ=255)
                                          aaCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGc                                               <  1:2949490/93‑1 (MQ=255)
                                          aaCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGc                                               >  2:3603229/1‑93 (MQ=255)
                                          aaCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGc                                               >  1:1969911/1‑93 (MQ=255)
                                           aCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGcc                                              >  2:3131905/1‑93 (MQ=255)
                                           aCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGcc                                              >  2:2446815/1‑93 (MQ=255)
                                           aCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGcc                                              >  2:4469189/1‑93 (MQ=255)
                                            cccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCa                                             <  2:2705950/93‑1 (MQ=255)
                                            cccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCa                                             <  2:3275879/93‑1 (MQ=255)
                                            cccGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCa                                             >  1:3023731/1‑93 (MQ=255)
                                            cccGCCAACCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCa                                             <  2:3364034/93‑1 (MQ=255)
                                              cGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAgg                                           <  2:3513182/93‑1 (MQ=255)
                                               gCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGgc                                          <  1:1134060/93‑1 (MQ=255)
                                                ccAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGgcg                                         >  1:4598236/1‑93 (MQ=255)
                                                 cAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGgcgc                                        >  2:3040369/1‑93 (MQ=255)
                                                  aaTCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCa                                       >  2:891352/1‑93 (MQ=255)
                                                   aTCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCAc                                      >  2:4641653/1‑93 (MQ=255)
                                                    tCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGgcgcgcg                                     >  2:1704945/1‑90 (MQ=255)
                                                    tCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCAcc                                     <  2:2083484/93‑1 (MQ=255)
                                                     cGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCg                                    <  2:4020245/93‑1 (MQ=255)
                                                      gACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGt                                   <  2:204767/93‑1 (MQ=255)
                                                       aCCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGtt                                  >  1:2857481/1‑93 (MQ=255)
                                                       aCCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGtt                                  >  1:4633827/1‑93 (MQ=255)
                                                        cccGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGttt                                 >  1:2762834/1‑93 (MQ=255)
                                                          cGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTaa                               <  2:4080487/93‑1 (MQ=255)
                                                          cGCTGCGTGAAGCGCATGTGATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTaa                               <  2:3349249/93‑1 (MQ=255)
                                                           gCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAg                              <  2:3724857/93‑1 (MQ=255)
                                                            cTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGc                             <  2:1935056/93‑1 (MQ=255)
                                                               cGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCttt                          <  1:2594519/93‑1 (MQ=255)
                                                                gTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTa                         >  1:1514235/1‑93 (MQ=255)
                                                                 ggAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTaa                        <  1:887232/92‑1 (MQ=255)
                                                                   aaGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAAt                      <  2:3960897/93‑1 (MQ=255)
                                                                   aaGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAAt                      >  2:3489046/1‑93 (MQ=255)
                                                                       gcATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGcc                  <  1:1719115/93‑1 (MQ=255)
                                                                        cATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCg                 >  1:2225876/1‑93 (MQ=255)
                                                                        cATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCg                 >  1:3466084/1‑93 (MQ=255)
                                                                        cATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCg                 >  2:1088682/1‑93 (MQ=255)
                                                                        cATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCg                 >  1:937507/1‑93 (MQ=255)
                                                                        cATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTATAAATCGCCg                 >  2:1595337/1‑93 (MQ=255)
                                                                         aTGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGa                >  1:552615/1‑93 (MQ=255)
                                                                         aTGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGa                >  1:2711891/1‑93 (MQ=255)
                                                                         aTGTTATGTCGCGCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGa                <  2:119396/93‑1 (MQ=255)
                                                                          tGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAt               >  1:4164138/1‑93 (MQ=255)
                                                                           gTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAtt              >  1:128114/1‑93 (MQ=255)
                                                                            ttATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTc             <  1:4536148/93‑1 (MQ=255)
                                                                            ttATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTc             <  2:2690365/93‑1 (MQ=255)
                                                                            ttATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTc             <  2:4196994/93‑1 (MQ=255)
                                                                            ttATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTc             <  2:1800613/93‑1 (MQ=255)
                                                                              aTGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTg           >  2:4231459/1‑93 (MQ=255)
                                                                              aTGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTg           >  1:4232363/1‑93 (MQ=255)
                                                                               tGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGc          <  1:2930819/93‑1 (MQ=255)
                                                                                 tcgctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGctc        <  1:3291337/93‑1 (MQ=255)
                                                                                 tcgctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGctc        <  2:2947564/93‑1 (MQ=255)
                                                                                 gcgctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGctc        <  2:1652713/92‑1 (MQ=255)
                                                                                  cgctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGctct       <  2:3096410/93‑1 (MQ=255)
                                                                                   gctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTa      >  1:1858797/1‑93 (MQ=255)
                                                                                    ctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTAc     <  1:2176436/93‑1 (MQ=255)
                                                                                    ctcgcCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTAc     <  1:4304619/93‑1 (MQ=255)
                                                                                    cgcgccgcCAGTATCGCGCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTAc     <  1:127438/91‑1 (MQ=255)
                                                                                      cgccgcCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTc   <  1:650840/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC  >  NC_000913/3353860‑3354039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  >  NC_000913/3353857‑3354043
                                                                                             |                                                                                             
CGCCTTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCG                                                                                               >  SRR4302535.2040508/1‑94 (MQ=60)
  CATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCC                                                                                             >  SRR4302535.1653928/1‑94 (MQ=60)
    TTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAG                                                                                           <  SRR4302535.4638454/94‑1 (MQ=60)
     TATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGT                                                                                          <  SRR4302535.3877851/94‑1 (MQ=60)
     TATTTACGACTACTGCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGT                                                                                          <  SRR4302535.3425624/94‑1 (MQ=60)
      TTTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTA                                                                                         <  SRR4302535.1882509/94‑1 (MQ=60)
      ATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTA                                                                                         >  SRR4302535.2004336/1‑94 (MQ=60)
        TTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATC                                                                                       >  SRR4302535.4520765/1‑94 (MQ=60)
          acgccacCTTCCGCCAGCAGTTTGCCCGGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGG                                                                                     <  SRR4302535.3317621/87‑1 (MQ=60)
           CGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGT                                                                                    >  SRR4302535.1908173/1‑94 (MQ=60)
            GACTCCTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTC                                                                                   <  SRR4302535.979479/94‑1 (MQ=60)
            GACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTC                                                                                   <  SRR4302535.3048708/94‑1 (MQ=60)
            GACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTC                                                                                   >  SRR4302535.1639600/1‑94 (MQ=60)
             ACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCGTGTTATGTCGCTCGCCGCCAGTATCCGTCG                                                                                  >  SRR4302535.1340394/1‑94 (MQ=60)
             ACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCG                                                                                  >  SRR4302535.4503190/1‑94 (MQ=60)
              CTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGT                                                                                 >  SRR4302535.4282996/1‑94 (MQ=60)
                ACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGA                                                                               >  SRR4302535.43844/1‑94 (MQ=60)
                ACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGA                                                                               >  SRR4302535.2751236/1‑94 (MQ=60)
                ACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGA                                                                               >  SRR4302535.3885597/1‑94 (MQ=60)
                ACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGA                                                                               >  SRR4302535.433318/1‑94 (MQ=60)
                   TCCGCCAGCAGTTTGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAAT                                                                            <  SRR4302535.850505/94‑1 (MQ=60)
                      GCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAA                                                                         >  SRR4302535.3372417/1‑94 (MQ=60)
                      GCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTCCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAA                                                                         >  SRR4302535.3329104/1‑94 (MQ=60)
                       CCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAAC                                                                        <  SRR4302535.4087520/94‑1 (MQ=60)
                        CAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACG                                                                       >  SRR4302535.615888/1‑94 (MQ=60)
                          GCATTTTGCCCAGCTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTC                                                                     <  SRR4302535.2481893/94‑1 (MQ=60)
                          GCAGTTTGCCAAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTC                                                                     <  SRR4302535.1919918/94‑1 (MQ=60)
                           CAGTTTGCCCGGGGGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCT                                                                    <  SRR4302535.3610341/94‑1 (MQ=60)
                            GGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTT                                                                   <  SRR4302535.4260882/94‑1 (MQ=60)
                            AGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGACATGAACGTCTT                                                                   >  SRR4302535.757944/1‑94 (MQ=60)
                            AGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTT                                                                   >  SRR4302535.494251/1‑94 (MQ=60)
                            AGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTT                                                                   >  SRR4302535.2458010/1‑94 (MQ=60)
                             GTTTGCCCAGGTGACTATCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTT                                                                  <  SRR4302535.1525974/94‑1 (MQ=60)
                             GTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTT                                                                  <  SRR4302535.4284359/94‑1 (MQ=60)
                              TTTCCCCGGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTT                                                                 <  SRR4302535.2186655/94‑1 (MQ=60)
                              TGTGCCCAGGTACCTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTT                                                                 <  SRR4302535.910895/94‑1 (MQ=60)
                               TTGCCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTG                                                                <  SRR4302535.1218967/94‑1 (MQ=60)
                               TTGCCCAGGTAACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTG                                                                <  SRR4302535.2547782/94‑1 (MQ=60)
                                 GCCCAGTTCACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCG                                                              <  SRR4302535.2449263/94‑1 (MQ=60)
                                 GCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCG                                                              >  SRR4302535.2080825/1‑94 (MQ=60)
                                  cgaaggtttcaccCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGGTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCTA                                                             <  SRR4302535.3538628/81‑1 (MQ=60)
                                  CCCAGGTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGA                                                             <  SRR4302535.2869979/94‑1 (MQ=60)
                                   CCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAA                                                            >  SRR4302535.2076609/1‑94 (MQ=60)
                                    CAGTTGACTACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAG                                                           <  SRR4302535.3993225/94‑1 (MQ=60)
                                    CAGTTGACTAACCCGCCAATCGCCCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAG                                                           <  SRR4302535.431436/94‑1 (MQ=60)
                                    CAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAG                                                           >  SRR4302535.2678704/1‑94 (MQ=60)
                                     AGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGC                                                          >  SRR4302535.1382804/1‑94 (MQ=60)
                                       gaggctacCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAG                                                        <  SRR4302535.1183705/86‑1 (MQ=60)
                                       GTAACTACCCCGCCAAGCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAG                                                        <  SRR4302535.2146259/94‑1 (MQ=60)
                                        TGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGG                                                       >  SRR4302535.1010808/1‑94 (MQ=60)
                                        TGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGA                                                       <  SRR4302535.1985633/94‑1 (MQ=60)
                                         GACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGTGAAGCAGAG                                                      >  SRR4302535.3821144/1‑94 (MQ=60)
                                          ACAAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGG                                                     <  SRR4302535.2308492/94‑1 (MQ=60)
                                           CTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGG                                                    <  SRR4302535.2394498/94‑1 (MQ=60)
                                            TACCCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGC                                                   <  SRR4302535.3845012/94‑1 (MQ=60)
                                            TAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGC                                                   >  SRR4302535.1984102/1‑94 (MQ=60)
                                            TAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGC                                                   >  SRR4302535.3137240/1‑94 (MQ=60)
                                             AACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCC                                                  <  SRR4302535.2970211/94‑1 (MQ=60)
                                             AACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCC                                                  <  SRR4302535.3940363/94‑1 (MQ=60)
                                              ACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCA                                                 >  SRR4302535.3044902/1‑94 (MQ=60)
                                                CCGCCAGTCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGG                                               <  SRR4302535.2232936/94‑1 (MQ=60)
                                                 CGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGC                                              >  SRR4302535.128642/1‑94 (MQ=60)
                                                  GCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCG                                             <  SRR4302535.1142153/94‑1 (MQ=60)
                                                  GCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCG                                             >  SRR4302535.4629517/1‑94 (MQ=60)
                                                   CCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGC                                            >  SRR4302535.1891863/1‑94 (MQ=60)
                                                         GACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTT                                      >  SRR4302535.2877723/1‑94 (MQ=60)
                                                         GACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTT                                      >  SRR4302535.4665260/1‑94 (MQ=60)
                                                          ACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTT                                     >  SRR4302535.2782518/1‑94 (MQ=60)
                                                            CCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAA                                   >  SRR4302535.1614636/1‑94 (MQ=60)
                                                            CCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAA                                   >  SRR4302535.4084402/1‑94 (MQ=60)
                                                             CGCTGCGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAG                                  <  SRR4302535.934576/94‑1 (MQ=60)
                                                                  CGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTA                             >  SRR4302535.1525141/1‑94 (MQ=60)
                                                                  CGTGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTA                             <  SRR4302535.2613158/94‑1 (MQ=60)
                                                                  CGTGAAGCGCATGTTATGGCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGCCTTTTGCGAAGCAGAGGGCCAGGCGCGCCGTGTAAGCTTTA                             >  SRR4302535.2532393/1‑94 (MQ=60)
                                                                    TGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAA                           <  SRR4302535.3515692/94‑1 (MQ=60)
                                                                    GGAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAA                           <  SRR4302535.893514/94‑1 (MQ=60)
                                                                     GAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAAT                          >  SRR4302535.4273037/1‑94 (MQ=60)
                                                                     GAAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAAT                          <  SRR4302535.4334262/94‑1 (MQ=60)
                                                                      AAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATC                         <  SRR4302535.4666879/94‑1 (MQ=60)
                                                                      AAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATC                         <  SRR4302535.1000880/94‑1 (MQ=60)
                                                                      AAGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATC                         <  SRR4302535.2395315/94‑1 (MQ=60)
                                                                       AGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCG                        <  SRR4302535.1873134/94‑1 (MQ=60)
                                                                       AGCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCG                        <  SRR4302535.2985352/94‑1 (MQ=60)
                                                                        GCGCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGC                       <  SRR4302535.2965124/94‑1 (MQ=60)
                                                                          GCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCG                     >  SRR4302535.961633/1‑94 (MQ=60)
                                                                          GCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCG                     >  SRR4302535.3490169/1‑94 (MQ=60)
                                                                          GCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCG                     >  SRR4302535.2241711/1‑94 (MQ=60)
                                                                          GCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCG                     >  SRR4302535.944147/1‑94 (MQ=60)
                                                                          GCATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCG                     <  SRR4302535.1731549/94‑1 (MQ=60)
                                                                           CATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGA                    >  SRR4302535.556486/1‑94 (MQ=60)
                                                                           CATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGA                    >  SRR4302535.2731271/1‑94 (MQ=60)
                                                                            ATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAT                   >  SRR4302535.757881/1‑94 (MQ=60)
                                                                            ATGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAT                   >  SRR4302535.4192648/1‑94 (MQ=60)
                                                                            AGGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGAT                   <  SRR4302535.1750094/94‑1 (MQ=60)
                                                                             TGTTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATT                  >  SRR4302535.129018/1‑94 (MQ=60)
                                                                               TTATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCT                <  SRR4302535.4567183/94‑1 (MQ=60)
                                                                                TATGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTG               >  SRR4302535.4261328/1‑94 (MQ=60)
                                                                                  TGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCT             <  SRR4302535.4346402/94‑1 (MQ=60)
                                                                                  TGTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCT             <  SRR4302535.2951459/94‑1 (MQ=60)
                                                                                   GTCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTC            <  SRR4302535.838305/94‑1 (MQ=60)
                                                                                    TCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCT           <  SRR4302535.50044/94‑1 (MQ=60)
                                                                                    TCGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCT           <  SRR4302535.3314222/94‑1 (MQ=60)
                                                                                     CGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTA          >  SRR4302535.1414909/1‑94 (MQ=60)
                                                                                     CGCTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTA          >  SRR4302535.1872201/1‑94 (MQ=60)
                                                                                       CTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACT        <  SRR4302535.4334069/94‑1 (MQ=60)
                                                                                       CTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACT        >  SRR4302535.4595902/1‑94 (MQ=60)
                                                                                       CTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACT        <  SRR4302535.2191992/94‑1 (MQ=60)
                                                                                       CTCGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACT        >  SRR4302535.1017303/1‑94 (MQ=60)
                                                                                       CGCGCCGCCAGTATCGCGCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACT        <  SRR4302535.128338/94‑1 (MQ=60)
                                                                                         CGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC      <  SRR4302535.2906796/94‑1 (MQ=60)
                                                                                         CGCCGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC      <  SRR4302535.655379/94‑1 (MQ=60)
                                                                                         CGCCGCCAGTATCGGTCGTGAAATGAACGGCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCC      <  SRR4302535.1274921/94‑1 (MQ=60)
                                                                                            CGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGAT   <  SRR4302535.2645625/94‑1 (MQ=60)
                                                                                            CGCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGAT   <  SRR4302535.3967139/94‑1 (MQ=60)
                                                                                             GCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  <  SRR4302535.3502913/94‑1 (MQ=60)
                                                                                             GCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  <  SRR4302535.2036647/94‑1 (MQ=60)
                                                                                             GCCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  >  SRR4302535.1664835/1‑94 (MQ=60)
                                                                                             CCCAGGATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  <  SRR4302535.92714/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGCATTATTTACGACTACTTCCGCCAGCAGTTTGCCCAGGTGACTAACCCGCCAATCGACCCGCTGCGTGAAGCGCATGTTATGTCGCTCGCCACCAGTATCGGTCGTGAAATGAACGTCTTTTGCGAAGCAGAGGGCCAGGCGCACCGTTTAAGCTTTAAATCGCCGATTCTGCTCTACTCCGATT  >  NC_000913/3353857‑3354043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑