Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,356,616 T→C A1450A (GCT→GCC)  gltB → glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,356,6160TC100.0% 1045.8 / NA 334A1450A (GCT→GCC) gltBglutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (166/168);  total (166/168)

Only 100 of 349 total aligned reads displayed.

GACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAA  >  NC_000913/3356526‑3356705
                                                                                          |                                                                                         
gACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGccct                                                                                         >  2:5471527/1‑93 (MQ=255)
 aCCGGCGGTTTCGGTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTg                                                                                        <  1:4649159/93‑1 (MQ=255)
 aCCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTg                                                                                        <  1:7679192/93‑1 (MQ=255)
  ccGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTgg                                                                                       >  1:1207217/1‑93 (MQ=255)
  ccGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTgg                                                                                       >  1:3989562/1‑93 (MQ=255)
  ccGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTgg                                                                                       >  1:6839317/1‑93 (MQ=255)
   cggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGc                                                                                      <  1:5073358/93‑1 (MQ=255)
     gcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGa                                                                                    >  1:7212267/1‑93 (MQ=255)
       ggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATc                                                                                  <  2:5675433/93‑1 (MQ=255)
        gTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATcc                                                                                 >  2:3988302/1‑93 (MQ=255)
        gTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATcc                                                                                 >  1:1768028/1‑93 (MQ=255)
        gTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATcc                                                                                 >  2:1876458/1‑93 (MQ=255)
         tttCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCa                                                                                <  2:2253112/93‑1 (MQ=255)
         tttCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCa                                                                                >  1:7274303/1‑93 (MQ=255)
         tttCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCa                                                                                >  1:1305007/1‑93 (MQ=255)
          ttCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCCAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCAt                                                                               >  1:2308558/1‑93 (MQ=255)
          ttCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCAt                                                                               <  2:6600202/93‑1 (MQ=255)
          ttCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCAt                                                                               <  1:7837760/93‑1 (MQ=255)
           tCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATg                                                                              <  2:5812022/93‑1 (MQ=255)
             gCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGaa                                                                            <  2:799158/93‑1 (MQ=255)
             gCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGaa                                                                            <  2:4764630/93‑1 (MQ=255)
             gCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGGCTTAAGCGTTGACGCCCTGGCGATCCATGaa                                                                            >  1:5541044/1‑93 (MQ=255)
              cTTACGTTCTCGATGAAAGCGGCTATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAag                                                                           <  2:4297671/93‑1 (MQ=255)
              cTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAag                                                                           >  2:5945812/1‑93 (MQ=255)
               ttACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAaga                                                                          >  1:2803481/1‑93 (MQ=255)
                 aCGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGc                                                                        <  1:5652369/93‑1 (MQ=255)
                   gTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCAt                                                                      <  1:2456195/93‑1 (MQ=255)
                   cttCTCGATGAAAGCGGCGATTTCCGCAAACGCTTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCAt                                                                      <  1:2736472/92‑1 (MQ=255)
                    ttCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTCAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATc                                                                     <  1:3374624/93‑1 (MQ=255)
                     tctcGATGAAAGCGGCGATTTCCGCAAACGCGTTAACACGGAACTGGTCGAGGTCTTAATCGTTGACGCCCTGGCGATCCATGAAGAGCATCt                                                                    >  2:4865544/1‑93 (MQ=255)
                      ctcGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTg                                                                   >  1:2940582/1‑93 (MQ=255)
                       tcGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGc                                                                  <  2:1087106/93‑1 (MQ=255)
                        cGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCg                                                                 <  1:4402372/93‑1 (MQ=255)
                         gATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGt                                                                >  1:6492083/1‑93 (MQ=255)
                         gATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGt                                                                >  1:6388668/1‑93 (MQ=255)
                          aTGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTg                                                               <  2:2488353/93‑1 (MQ=255)
                            gAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGt                                                             <  1:2075201/93‑1 (MQ=255)
                            gAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGt                                                             >  2:7080473/1‑93 (MQ=255)
                              aaGCGGCGATTTCCGCAAACGCGTTAACCCGGACCTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATTCATGAAGAGCATCTGCGTGGTCt                                                           <  2:816555/93‑1 (MQ=255)
                              aaGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCt                                                           >  1:7778082/1‑93 (MQ=255)
                                gcggcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTa                                                         >  2:2894656/1‑93 (MQ=255)
                                gcggcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTa                                                         <  1:1601734/93‑1 (MQ=255)
                                gcggcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTa                                                         <  1:4999869/93‑1 (MQ=255)
                                   gcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGCAGAGCATCTGCGTGGTCTTATCa                                                      >  2:3206374/1‑93 (MQ=255)
                                   gcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCa                                                      >  2:4030347/1‑93 (MQ=255)
                                   gcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCa                                                      >  2:3133464/1‑93 (MQ=255)
                                    cgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCAc                                                     <  1:3567228/93‑1 (MQ=255)
                                     gATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCAcc                                                    <  2:5172778/93‑1 (MQ=255)
                                     gATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCAcc                                                    >  2:7638601/1‑93 (MQ=255)
                                      aTTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCg                                                   <  1:4276077/93‑1 (MQ=255)
                                      aTTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCg                                                   >  2:6214917/1‑93 (MQ=255)
                                       tttCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGa                                                  <  1:4078552/93‑1 (MQ=255)
                                       tttCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGa                                                  <  1:3247703/93‑1 (MQ=255)
                                       tttCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGa                                                  <  2:6901113/93‑1 (MQ=255)
                                          ccGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCa                                               <  2:1812439/93‑1 (MQ=255)
                                           cGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAt                                              <  2:2113290/93‑1 (MQ=255)
                                            gCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAtg                                             >  1:5814164/1‑93 (MQ=255)
                                             cAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAtgt                                            >  2:6147825/1‑93 (MQ=255)
                                             cAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAtgt                                            >  2:4696329/1‑93 (MQ=255)
                                              aaaCGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAtgtg                                           <  1:3185088/93‑1 (MQ=255)
                                               aaCGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgc                                          >  1:2601306/1‑93 (MQ=255)
                                               aaCGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgc                                          <  1:1374951/93‑1 (MQ=255)
                                                   cgTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgcagca                                      <  2:2965468/93‑1 (MQ=255)
                                                    gTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAt                                     >  2:1572772/1‑93 (MQ=255)
                                                    gTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAt                                     >  1:1478786/1‑93 (MQ=255)
                                                    gTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAt                                     >  2:536861/1‑93 (MQ=255)
                                                     ttAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATa                                    >  2:3700217/1‑93 (MQ=255)
                                                     ttAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATa                                    >  1:6213541/1‑93 (MQ=255)
                                                     ttAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATa                                    >  1:2208448/1‑93 (MQ=255)
                                                     ttAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATa                                    >  2:7277697/1‑93 (MQ=255)
                                                      tAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATAc                                   <  1:1143160/93‑1 (MQ=255)
                                                       aaCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATAcc                                  <  2:419561/93‑1 (MQ=255)
                                                       aaCCCGGAACTGGTCGAGGTCTTAAGCGTCGCCGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATAcc                                  <  1:5247611/93‑1 (MQ=255)
                                                           cGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGct                              <  2:145044/93‑1 (MQ=255)
                                                            ggAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGctc                             <  1:6251539/93‑1 (MQ=255)
                                                              aaCTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCtctc                           >  1:363371/1‑93 (MQ=255)
                                                               aCTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCa                          <  1:6007156/93‑1 (MQ=255)
                                                               aCTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCa                          <  2:2280940/93‑1 (MQ=255)
                                                               aCTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCa                          >  2:2797793/1‑93 (MQ=255)
                                                                 tGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAgc                        >  1:1652678/1‑93 (MQ=255)
                                                                  ggTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAgcg                       >  2:2008838/1‑93 (MQ=255)
                                                                      gAGGTCTTAAGCGTTGATGCCCTGGCGATCCATGAATAGCATCTGCGTGGTCTTATCACCGAGCATGTGCTGCATACCGGCTCTCAGCGCGGt                   <  2:4977556/93‑1 (MQ=255)
                                                                      gAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGt                   <  1:4292515/93‑1 (MQ=255)
                                                                      gAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGt                   >  2:5439307/1‑93 (MQ=255)
                                                                       aGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTg                  >  2:7425797/1‑93 (MQ=255)
                                                                       aGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTg                  >  1:1908230/1‑93 (MQ=255)
                                                                         gTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGaa                <  1:4536673/93‑1 (MQ=255)
                                                                         gTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGaa                >  1:7585717/1‑93 (MQ=255)
                                                                          tCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAag               >  2:3765441/1‑93 (MQ=255)
                                                                            ttAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAagag             >  2:6564777/1‑93 (MQ=255)
                                                                            ttAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAagag             >  2:1176827/1‑93 (MQ=255)
                                                                            ttAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAagag             >  2:3362484/1‑93 (MQ=255)
                                                                             tAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAgaga            >  1:3851969/1‑93 (MQ=255)
                                                                             tAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAgaga            >  2:2506850/1‑93 (MQ=255)
                                                                              aaGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAt           <  1:864181/93‑1 (MQ=255)
                                                                                 cGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCt        <  2:7274302/93‑1 (MQ=255)
                                                                                 cGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCt        <  1:6648662/93‑1 (MQ=255)
                                                                                  gTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTg       <  2:6373186/93‑1 (MQ=255)
                                                                                  gTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTg       >  1:5340299/1‑93 (MQ=255)
                                                                                   ttGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTgg      >  1:4704568/1‑93 (MQ=255)
                                                                                          |                                                                                         
GACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAA  >  NC_000913/3356526‑3356705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 202 total aligned reads displayed.
CATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  NC_000913/3356523‑3356709
                                                                                             |                                                                                             
CATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCC                                                                                               <  SRR4302543.372477/94‑1 (MQ=60)
CATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCC                                                                                               >  SRR4302543.1756080/1‑94 (MQ=60)
 ATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCC                                                                                              >  SRR4302543.1111149/1‑94 (MQ=60)
 ATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCC                                                                                              >  SRR4302543.1472823/1‑94 (MQ=60)
 ATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGCATTAAGCGTTGACGCCC                                                                                              >  SRR4302543.4300463/1‑94 (MQ=60)
  TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                             >  SRR4302543.3640885/1‑94 (MQ=60)
  TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                             <  SRR4302543.7293907/94‑1 (MQ=60)
  TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                             <  SRR4302543.68897/94‑1 (MQ=60)
   GACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTG                                                                                            <  SRR4302543.3960134/94‑1 (MQ=60)
    ACCGGCGGTTTCGGTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           <  SRR4302543.4691467/94‑1 (MQ=60)
    ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           <  SRR4302543.2699245/94‑1 (MQ=60)
    ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           >  SRR4302543.6903872/1‑94 (MQ=60)
    ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           >  SRR4302543.1218077/1‑94 (MQ=60)
    ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           <  SRR4302543.3010008/94‑1 (MQ=60)
    ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           >  SRR4302543.4025023/1‑94 (MQ=60)
    ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                           <  SRR4302543.7751934/94‑1 (MQ=60)
     CCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGC                                                                                          >  SRR4302543.2082628/1‑94 (MQ=60)
     CCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGC                                                                                          >  SRR4302543.5098518/1‑94 (MQ=60)
     CCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGAGTTCTGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGC                                                                                          >  SRR4302543.7876721/1‑94 (MQ=60)
      CGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCG                                                                                         <  SRR4302543.5119395/94‑1 (MQ=60)
      CGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCG                                                                                         <  SRR4302543.7770057/94‑1 (MQ=60)
      CGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCG                                                                                         <  SRR4302543.7770058/94‑1 (MQ=60)
      CGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCG                                                                                         <  SRR4302543.1397705/94‑1 (MQ=60)
      CGGCGGTTTCGCTTACGTTCTCGATGAAAGCAGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCG                                                                                         >  SRR4302543.1220799/1‑94 (MQ=60)
       GGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGA                                                                                        >  SRR4302543.7282180/1‑94 (MQ=60)
        GCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGAT                                                                                       <  SRR4302543.6532905/94‑1 (MQ=60)
         CGGTTTCGCTTACGTTCTCGATGGAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATC                                                                                      >  SRR4302543.3327739/1‑94 (MQ=60)
          gtttttgattaccTTCTCGATGAAAGCGGCGATTTGCCCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCC                                                                                     <  SRR4302543.5934317/81‑1 (MQ=60)
          GGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCC                                                                                     <  SRR4302543.2258107/94‑1 (MQ=60)
          GGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCC                                                                                     >  SRR4302543.1783839/1‑94 (MQ=60)
          GGTTTCGCTTACGTTCTCGATGAAAGCGACGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCC                                                                                     >  SRR4302543.1351708/1‑94 (MQ=60)
           GTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCA                                                                                    >  SRR4302543.7344566/1‑94 (MQ=60)
           GTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCA                                                                                    >  SRR4302543.1316596/1‑94 (MQ=60)
           GTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCA                                                                                    >  SRR4302543.1645067/1‑94 (MQ=60)
            TTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCCAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCAT                                                                                   >  SRR4302543.2329576/1‑94 (MQ=60)
            TTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCAT                                                                                   <  SRR4302543.7489321/94‑1 (MQ=60)
             TTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATG                                                                                  <  SRR4302543.7911526/94‑1 (MQ=60)
               CGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAA                                                                                <  SRR4302543.4734135/94‑1 (MQ=60)
               CGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAA                                                                                <  SRR4302543.6684011/94‑1 (MQ=60)
               CGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAA                                                                                >  SRR4302543.7686497/1‑94 (MQ=60)
               CGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAA                                                                                >  SRR4302543.6975783/1‑94 (MQ=60)
               CGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAA                                                                                >  SRR4302543.508671/1‑94 (MQ=60)
               CGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGGCTTAAGCGTTGACGCCCTGGCGATCCATGAA                                                                                >  SRR4302543.5591426/1‑94 (MQ=60)
                GCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAG                                                                               <  SRR4302543.5280064/94‑1 (MQ=60)
                 CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGA                                                                              >  SRR4302543.7183852/1‑94 (MQ=60)
                 CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGA                                                                              <  SRR4302543.1758892/94‑1 (MQ=60)
                 CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGA                                                                              >  SRR4302543.2829118/1‑94 (MQ=60)
                 CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTCGACGCCCTGGCGATGCATGAAGA                                                                              >  SRR4302543.1851498/1‑94 (MQ=60)
                  TTACGTTCTCGATGAAAGCGGCGATTCCCGCAACCGCGTTACCCCGGAACTGGTCGAGGTCTTAAGGGTTGACGCCCTGGCGATCCATGAAGAG                                                                             <  SRR4302543.1822389/94‑1 (MQ=60)
                   TACGTGCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGC                                                                            <  SRR4302543.35471/94‑1 (MQ=60)
                    ACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCA                                                                           <  SRR4302543.5703905/94‑1 (MQ=60)
                      GTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATC                                                                         <  SRR4302543.2478343/94‑1 (MQ=60)
                      GTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATC                                                                         >  SRR4302543.579230/1‑94 (MQ=60)
                      GTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATC                                                                         <  SRR4302543.2761369/94‑1 (MQ=60)
                      GTTCTCGATGAAAGCGGCGATTTCCACAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGActctc                                                                         >  SRR4302543.3753807/1‑89 (MQ=60)
                      CTTCTCGATGAAAGCGGCGATTTCCGCAAACGCTTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATC                                                                         <  SRR4302543.2761370/94‑1 (MQ=60)
                       TTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCT                                                                        >  SRR4302543.1613120/1‑94 (MQ=60)
                       TTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTCAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCT                                                                        <  SRR4302543.3404921/94‑1 (MQ=60)
                        TCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTG                                                                       <  SRR4302543.6181432/94‑1 (MQ=60)
                        TCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTG                                                                       <  SRR4302543.1344485/94‑1 (MQ=60)
                        TCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTG                                                                       >  SRR4302543.2967281/1‑94 (MQ=60)
                        TCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTG                                                                       >  SRR4302543.2967282/1‑94 (MQ=60)
                         CTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGC                                                                      >  SRR4302543.6275019/1‑94 (MQ=60)
                         CTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGACCTGGCGATCCATGAAGAGCATCTGC                                                                      >  SRR4302543.3188713/1‑94 (MQ=60)
                           CGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGT                                                                    >  SRR4302543.6448803/1‑94 (MQ=60)
                           CGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGT                                                                    >  SRR4302543.6553244/1‑94 (MQ=60)
                           CGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGT                                                                    <  SRR4302543.4442280/94‑1 (MQ=60)
                           CGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGT                                                                    >  SRR4302543.1657825/1‑94 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                  >  SRR4302543.4456483/1‑94 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                  <  SRR4302543.4539677/94‑1 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                  >  SRR4302543.941058/1‑94 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                  >  SRR4302543.2912907/1‑94 (MQ=60)
                             ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                  <  SRR4302543.1183490/94‑1 (MQ=60)
                              TGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGT                                                                 <  SRR4302543.1576683/94‑1 (MQ=60)
                               GAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTC                                                                <  SRR4302543.2093739/94‑1 (MQ=60)
                               GAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTC                                                                <  SRR4302543.7912206/94‑1 (MQ=60)
                               GAAAGCGGCGATTCCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTC                                                                <  SRR4302543.6508526/94‑1 (MQ=60)
                                AAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCT                                                               <  SRR4302543.4257686/94‑1 (MQ=60)
                                AAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCT                                                               >  SRR4302543.7851432/1‑94 (MQ=60)
                                  AGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTA                                                             >  SRR4302543.2268746/1‑94 (MQ=60)
                                  AGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTA                                                             >  SRR4302543.4225673/1‑94 (MQ=60)
                                   GCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTAT                                                            <  SRR4302543.5045375/94‑1 (MQ=60)
                                   GCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTAT                                                            <  SRR4302543.7509171/94‑1 (MQ=60)
                                   GCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTAT                                                            <  SRR4302543.1616033/94‑1 (MQ=60)
                                     GGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCA                                                          <  SRR4302543.1940066/94‑1 (MQ=60)
                                       CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                        >  SRR4302543.4092002/1‑94 (MQ=60)
                                       CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                        >  SRR4302543.4325256/1‑94 (MQ=60)
                                       CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                        <  SRR4302543.1199969/94‑1 (MQ=60)
                                       CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                        <  SRR4302543.3598779/94‑1 (MQ=60)
                                       CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                        <  SRR4302543.2538580/94‑1 (MQ=60)
                                        GATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCG                                                       >  SRR4302543.7906345/1‑94 (MQ=60)
                                        GATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCG                                                       <  SRR4302543.806007/94‑1 (MQ=60)
                                         ATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGA                                                      >  SRR4302543.4865895/1‑94 (MQ=60)
                                         ATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGA                                                      <  SRR4302543.4314942/94‑1 (MQ=60)
                                         ATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGA                                                      >  SRR4302543.4049889/1‑94 (MQ=60)
                                          TTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAG                                                     <  SRR4302543.4115115/94‑1 (MQ=60)
                                          TTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAG                                                     <  SRR4302543.3277062/94‑1 (MQ=60)
                                          TTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAG                                                     <  SRR4302543.6319028/94‑1 (MQ=60)
                                           TTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGGCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGC                                                    <  SRR4302543.1508440/94‑1 (MQ=60)
                                            TCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCA                                                   <  SRR4302543.6356517/94‑1 (MQ=60)
                                             CCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAT                                                  <  SRR4302543.2179609/94‑1 (MQ=60)
                                             CCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAT                                                  <  SRR4302543.5755625/94‑1 (MQ=60)
                                              CGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATG                                                 <  SRR4302543.5561555/94‑1 (MQ=60)
                                              CGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATG                                                 <  SRR4302543.3900415/94‑1 (MQ=60)
                                              CGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATG                                                 >  SRR4302543.5867033/1‑94 (MQ=60)
                                                 AAACGCGTTAGCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGC                                              <  SRR4302543.2324232/94‑1 (MQ=60)
                                                 AAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGC                                              >  SRR4302543.3248510/1‑94 (MQ=60)
                                                 AAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGC                                              <  SRR4302543.3213814/94‑1 (MQ=60)
                                                 AAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGC                                              >  SRR4302543.2624933/1‑94 (MQ=60)
                                                 AAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGC                                              >  SRR4302543.1354841/1‑94 (MQ=60)
                                                  AACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCA                                             <  SRR4302543.1387305/94‑1 (MQ=60)
                                                   ACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAG                                            <  SRR4302543.4117495/94‑1 (MQ=60)
                                                    CGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGC                                           >  SRR4302543.4638574/1‑94 (MQ=60)
                                                    CGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGC                                           >  SRR4302543.6837833/1‑94 (MQ=60)
                                                    CGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGC                                           >  SRR4302543.335212/1‑94 (MQ=60)
                                                      CGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAT                                         <  SRR4302543.5961829/94‑1 (MQ=60)
                                                      CGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAT                                         >  SRR4302543.1492111/1‑94 (MQ=60)
                                                       GTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATA                                        >  SRR4302543.6272203/1‑94 (MQ=60)
                                                       GTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATA                                        >  SRR4302543.2228342/1‑94 (MQ=60)
                                                       GTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATA                                        >  SRR4302543.1689953/1‑94 (MQ=60)
                                                         TAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACC                                      <  SRR4302543.3165605/94‑1 (MQ=60)
                                                         TAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACC                                      <  SRR4302543.1153576/94‑1 (MQ=60)
                                                          AACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCG                                     <  SRR4302543.7289766/94‑1 (MQ=60)
                                                          AACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCG                                     >  SRR4302543.6791898/1‑94 (MQ=60)
                                                          AACCCGGAACTGGTCGAGGTCTTAAGCGTCGCCGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCG                                     <  SRR4302543.5294912/94‑1 (MQ=60)
                                                          AACCCGGAACTGGTCGAGGTCTTAAGCGGTGACGCCCTGGCGATCCATGAAGAGAATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCG                                     >  SRR4302543.2485204/1‑94 (MQ=60)
                                                            CCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGC                                   >  SRR4302543.3645717/1‑94 (MQ=60)
                                                            CCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGC                                   >  SRR4302543.4356928/1‑94 (MQ=60)
                                                             CCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCT                                  <  SRR4302543.255382/94‑1 (MQ=60)
                                                             CCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCT                                  >  SRR4302543.5488086/1‑94 (MQ=60)
                                                              CGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTC                                 >  SRR4302543.4328717/1‑94 (MQ=60)
                                                              CGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTC                                 <  SRR4302543.4011648/94‑1 (MQ=60)
                                                              CGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTC                                 <  SRR4302543.7327248/94‑1 (MQ=60)
                                                              CGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGAATGTGCAGCATACCGGCTC                                 >  SRR4302543.5700573/1‑94 (MQ=60)
                                                               GGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCT                                <  SRR4302543.6310512/94‑1 (MQ=60)
                                                               GGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCT                                <  SRR4302543.6386284/94‑1 (MQ=60)
                                                               GGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCT                                >  SRR4302543.5330759/1‑94 (MQ=60)
                                                               GGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCT                                >  SRR4302543.5187179/1‑94 (MQ=60)
                                                               GGAACTGGTCGAGGTCTTAAGCATTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCT                                <  SRR4302543.4426693/94‑1 (MQ=60)
                                                                GAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTC                               >  SRR4302543.366736/1‑94 (MQ=60)
                                                                 AACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCA                              <  SRR4302543.2332437/94‑1 (MQ=60)
                                                                 AACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCA                              <  SRR4302543.5640045/94‑1 (MQ=60)
                                                                  ACTGGTCGAGGTTTTAAGCGTTGCCGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAG                             <  SRR4302543.2186909/94‑1 (MQ=60)
                                                                  ACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAG                             >  SRR4302543.6581316/1‑94 (MQ=60)
                                                                  ACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAG                             <  SRR4302543.6062474/94‑1 (MQ=60)
                                                                   CTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGC                            >  SRR4302543.1667460/1‑94 (MQ=60)
                                                                    TGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCG                           >  SRR4302543.6581231/1‑94 (MQ=60)
                                                                     GGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGC                          >  SRR4302543.7605123/1‑94 (MQ=60)
                                                                     GGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGC                          >  SRR4302543.3317544/1‑94 (MQ=60)
                                                                        CGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGT                       <  SRR4302543.2807087/94‑1 (MQ=60)
                                                                        CGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGT                       >  SRR4302543.1689516/1‑94 (MQ=60)
                                                                         GAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTG                      >  SRR4302543.1925419/1‑94 (MQ=60)
                                                                         GAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTG                      <  SRR4302543.4331542/94‑1 (MQ=60)
                                                                           GGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAA                    <  SRR4302543.3558939/94‑1 (MQ=60)
                                                                           GGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAA                    >  SRR4302543.4394562/1‑94 (MQ=60)
                                                                           GGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAA                    >  SRR4302543.7657808/1‑94 (MQ=60)
                                                                           GGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAA                    <  SRR4302543.5208685/94‑1 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   >  SRR4302543.3684785/1‑94 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   <  SRR4302543.6176063/94‑1 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   >  SRR4302543.4724160/1‑94 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   >  SRR4302543.365402/1‑94 (MQ=60)
                                                                            GTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAG                   <  SRR4302543.4577970/94‑1 (MQ=60)
                                                                             TCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGA                  <  SRR4302543.4674508/94‑1 (MQ=60)
                                                                              CTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAG                 >  SRR4302543.2370648/1‑94 (MQ=60)
                                                                              CTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAG                 >  SRR4302543.327561/1‑94 (MQ=60)
                                                                              CTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAG                 >  SRR4302543.5721670/1‑94 (MQ=60)
                                                                              CTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAG                 <  SRR4302543.2038143/94‑1 (MQ=60)
                                                                               TTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGA                <  SRR4302543.559084/94‑1 (MQ=60)
                                                                               TTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGA                >  SRR4302543.3886003/1‑94 (MQ=60)
                                                                                TAAGCGTTGACGCCCTGGCGATCCATGAAGAGCCTCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAT               <  SRR4302543.7690613/94‑1 (MQ=60)
                                                                                TAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAT               <  SRR4302543.7137820/94‑1 (MQ=60)
                                                                                TAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAT               <  SRR4302543.6256482/94‑1 (MQ=60)
                                                                                 AAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATT              <  SRR4302543.871891/94‑1 (MQ=60)
                                                                                  AGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTC             <  SRR4302543.4935834/94‑1 (MQ=60)
                                                                                  AGCGCTGCCGCCCTGGCGATCCATGAAGAGCATCTGCGTGGACTTATCACCGAGCAAGTGCAGCATACCGGCTCGCAGCGCGGTGAAGAGATTC             <  SRR4302543.1999585/94‑1 (MQ=60)
                                                                                   GCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCT            <  SRR4302543.1307353/94‑1 (MQ=60)
                                                                                    CGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTG           <  SRR4302543.6291036/94‑1 (MQ=60)
                                                                                    CGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTG           <  SRR4302543.536996/94‑1 (MQ=60)
                                                                                    CGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTG           >  SRR4302543.5388610/1‑94 (MQ=60)
                                                                                    CGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTG           <  SRR4302543.6711420/94‑1 (MQ=60)
                                                                                    CGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTG           <  SRR4302543.5928768/94‑1 (MQ=60)
                                                                                     GTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGG          >  SRR4302543.4747551/1‑94 (MQ=60)
                                                                                     GTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGG          >  SRR4302543.1482966/1‑94 (MQ=60)
                                                                                     GTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGG          >  SRR4302543.2789435/1‑94 (MQ=60)
                                                                                     GTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGG          >  SRR4302543.4884182/1‑94 (MQ=60)
                                                                                      TTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGC         >  SRR4302543.3735464/1‑94 (MQ=60)
                                                                                       TGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCG        >  SRR4302543.2766748/1‑94 (MQ=60)
                                                                                       TGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCG        >  SRR4302543.1002210/1‑94 (MQ=60)
                                                                                       TGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCG        >  SRR4302543.7764615/1‑94 (MQ=60)
                                                                                          CGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAAC     <  SRR4302543.4623138/94‑1 (MQ=60)
                                                                                           GCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACT    >  SRR4302543.347573/1‑94 (MQ=60)
                                                                                            CCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTG   <  SRR4302543.1142042/94‑1 (MQ=60)
                                                                                             CCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  SRR4302543.7690365/1‑94 (MQ=60)
                                                                                             CCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  SRR4302543.2186381/1‑94 (MQ=60)
                                                                                             CCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  SRR4302543.660888/1‑94 (MQ=60)
                                                                                             CCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  <  SRR4302543.2353162/94‑1 (MQ=60)
                                                                                             CCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  SRR4302543.5294721/1‑94 (MQ=60)
                                                                                             CCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  SRR4302543.4933951/1‑94 (MQ=60)
                                                                                             CCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  SRR4302543.1091017/1‑94 (MQ=60)
                                                                                             CCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGAGGACTGG  >  SRR4302543.6567847/1‑94 (MQ=60)
                                                                                             |                                                                                             
CATGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTGG  >  NC_000913/3356523‑3356709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑