Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,356,616 T→C A1450A (GCT→GCC)  gltB → glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,356,6160TC100.0% 597.7 / NA 191A1450A (GCT→GCC) gltBglutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (113/78);  total (113/78)

Only 100 of 200 total aligned reads displayed.

GACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAA  >  NC_000913/3356526‑3356705
                                                                                          |                                                                                         
gACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGccct                                                                                         >  1:1388403/1‑93 (MQ=255)
gACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGccct                                                                                         <  2:3193729/93‑1 (MQ=255)
 aCCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTg                                                                                        <  1:860715/93‑1 (MQ=255)
 aCCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTg                                                                                        <  2:354424/93‑1 (MQ=255)
   cggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGc                                                                                      >  2:524021/1‑93 (MQ=255)
   cggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGc                                                                                      >  2:2425123/1‑93 (MQ=255)
   cggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGc                                                                                      >  1:2073121/1‑93 (MQ=255)
   cgccggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGc                                                                                      <  2:4150693/90‑1 (MQ=255)
    ggcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCg                                                                                     >  1:3798336/1‑93 (MQ=255)
     gcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGa                                                                                    >  1:4607821/1‑93 (MQ=255)
     gcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGa                                                                                    >  1:1028770/1‑93 (MQ=255)
     gcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGa                                                                                    >  2:61315/1‑93 (MQ=255)
     gcggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGGCTTAAGCGTTGACGCCCTGGCGa                                                                                    >  2:2819347/1‑93 (MQ=255)
       ggTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATc                                                                                  <  2:1643435/93‑1 (MQ=255)
        gTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATcc                                                                                 >  1:1425051/1‑93 (MQ=255)
         tttCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCAGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCa                                                                                <  1:3297451/93‑1 (MQ=255)
          ttCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCAt                                                                               <  1:2313542/93‑1 (MQ=255)
            cGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGa                                                                             <  1:3513303/93‑1 (MQ=255)
             gCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGaa                                                                            <  2:1164906/93‑1 (MQ=255)
              cTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAag                                                                           <  1:1401108/93‑1 (MQ=255)
               ttACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAaga                                                                          <  2:675390/93‑1 (MQ=255)
               ttACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAaga                                                                          >  1:950241/1‑93 (MQ=255)
                tACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAatag                                                                         >  1:1771517/1‑93 (MQ=255)
                tACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAagag                                                                         <  2:3777579/93‑1 (MQ=255)
                  cGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCa                                                                       <  2:3683288/93‑1 (MQ=255)
                  cGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCa                                                                       <  1:3429599/93‑1 (MQ=255)
                   gTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCAt                                                                      >  2:2998661/1‑93 (MQ=255)
                    ttCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATc                                                                     >  2:1734663/1‑93 (MQ=255)
                    ttCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATc                                                                     <  1:163652/93‑1 (MQ=255)
                     tctcGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCt                                                                    >  2:3753406/1‑93 (MQ=255)
                       tcGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGc                                                                  <  1:3530056/93‑1 (MQ=255)
                        cGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCg                                                                 >  1:2456471/1‑93 (MQ=255)
                         gATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGt                                                                >  2:3943819/1‑93 (MQ=255)
                          aTGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTg                                                               >  2:1101161/1‑93 (MQ=255)
                          aTGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTg                                                               <  1:1157198/93‑1 (MQ=255)
                          aTGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTg                                                               >  1:1662482/1‑93 (MQ=255)
                           tGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTTACGCCCTGGCGATCCATGAAGAGCATCTGCGTgg                                                              >  2:4527194/1‑93 (MQ=255)
                           tGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGTCGCCCTGGCGATCCATGAAGAGCATCTGCGTgt                                                              >  1:3952256/1‑92 (MQ=255)
                            gAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGt                                                             >  2:107211/1‑93 (MQ=255)
                             aaaGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTc                                                            >  2:294260/1‑93 (MQ=255)
                              aaGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTCCGTGGTCt                                                           >  2:2240157/1‑93 (MQ=255)
                                 cggcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTAt                                                        <  2:2129378/93‑1 (MQ=255)
                                 cggcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTAt                                                        <  2:3440847/93‑1 (MQ=255)
                                   gcgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCa                                                      >  2:4468927/1‑93 (MQ=255)
                                    cgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACTCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCAc                                                     <  1:694783/93‑1 (MQ=255)
                                    cgATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCAc                                                     <  1:397504/93‑1 (MQ=255)
                                     gATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCAcc                                                    >  2:1588809/1‑93 (MQ=255)
                                      aTTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTGAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCg                                                   >  2:3828766/1‑93 (MQ=255)
                                      aTTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCg                                                   >  2:1230939/1‑93 (MQ=255)
                                          ccGCAAACGCGTTAACCGGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCa                                               <  2:2688662/93‑1 (MQ=255)
                                           cGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAt                                              >  1:1443076/1‑93 (MQ=255)
                                           cGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAt                                              <  2:2649880/93‑1 (MQ=255)
                                            gCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAtg                                             <  2:3517824/93‑1 (MQ=255)
                                            gCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAtg                                             <  1:544320/93‑1 (MQ=255)
                                               aaCGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgc                                          >  2:2247093/1‑93 (MQ=255)
                                                  gcgTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgcagc                                       >  2:2503008/1‑93 (MQ=255)
                                                   cgTTACCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTgcagca                                      <  1:3944094/93‑1 (MQ=255)
                                                    gTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGGAGCAt                                     >  2:4396051/1‑93 (MQ=255)
                                                    gTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAt                                     >  2:2074450/1‑93 (MQ=255)
                                                    gTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAt                                     >  2:4271367/1‑93 (MQ=255)
                                                    gTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATATGCAGCAt                                     >  1:2195766/1‑93 (MQ=255)
                                                     ttAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATa                                    >  2:1747178/1‑93 (MQ=255)
                                                       aaCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATAcc                                  <  1:3375727/93‑1 (MQ=255)
                                                         cccGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCgg                                <  1:2914440/93‑1 (MQ=255)
                                                         cccGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCgg                                >  2:3050343/1‑93 (MQ=255)
                                                          ccGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGc                               <  2:4687794/93‑1 (MQ=255)
                                                            ggAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGctc                             <  2:4315558/93‑1 (MQ=255)
                                                            ggAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGctc                             <  2:3622064/93‑1 (MQ=255)
                                                              aaCTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCtctc                           <  2:1951385/93‑1 (MQ=255)
                                                              aaCTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCtctc                           >  1:4343490/1‑93 (MQ=255)
                                                                cTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAg                         >  2:701595/1‑93 (MQ=255)
                                                                 tGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAgc                        <  2:808173/93‑1 (MQ=255)
                                                                 tGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAgc                        <  1:1598338/93‑1 (MQ=255)
                                                                   gTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAgcgc                      >  1:423530/1‑93 (MQ=255)
                                                                   gTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAgcgc                      <  1:3634302/93‑1 (MQ=255)
                                                                     cGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCgg                    <  1:837555/93‑1 (MQ=255)
                                                                     cGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCgg                    >  2:459703/1‑93 (MQ=255)
                                                                      gAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGt                   >  1:324965/1‑93 (MQ=255)
                                                                      gAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGt                   <  1:4575631/93‑1 (MQ=255)
                                                                      gAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGt                   >  1:3900491/1‑93 (MQ=255)
                                                                      caGGTCTTAAGCGTTGGCGCCCTCGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGt                   <  2:3894202/92‑1 (MQ=255)
                                                                       aGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTg                  <  1:2681678/93‑1 (MQ=255)
                                                                        ggTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGa                 >  1:2562017/1‑93 (MQ=255)
                                                                        ggTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGa                 >  1:1266870/1‑93 (MQ=255)
                                                                        ggTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGa                 >  2:1985015/1‑93 (MQ=255)
                                                                         gTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCCGCGCGGTGaa                >  2:399191/1‑93 (MQ=255)
                                                                         gTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGaa                >  2:3232296/1‑93 (MQ=255)
                                                                         gTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGaa                >  1:1815253/1‑93 (MQ=255)
                                                                          tCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAag               <  2:1347433/93‑1 (MQ=255)
                                                                           cTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAaga              >  2:1563829/1‑93 (MQ=255)
                                                                           cTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAaga              <  2:914872/93‑1 (MQ=255)
                                                                           cTTAAGCGTTCACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAaga              <  2:1422427/93‑1 (MQ=255)
                                                                             tAAGCGTGGTCGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAgaga            <  1:3055651/93‑1 (MQ=255)
                                                                               aGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAtt          >  1:1935222/1‑93 (MQ=255)
                                                                                gCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTc         >  1:1503363/1‑93 (MQ=255)
                                                                                  gTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTg       >  1:4205343/1‑93 (MQ=255)
                                                                                  gTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTg       <  1:1606945/93‑1 (MQ=255)
                                                                                   ttGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTgg      <  2:212193/93‑1 (MQ=255)
                                                                                   ttGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTgg      <  2:1498957/93‑1 (MQ=255)
                                                                                       cGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGaa  <  1:2297095/93‑1 (MQ=255)
                                                                                          |                                                                                         
GACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAA  >  NC_000913/3356526‑3356705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTG  >  NC_000913/3356525‑3356708
                                                                                           |                                                                                            
TGACCGGGGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                            <  SRR4302535.3905786/94‑1 (MQ=60)
TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                            <  SRR4302535.1796425/94‑1 (MQ=60)
TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                            <  SRR4302535.2934753/94‑1 (MQ=60)
TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                            <  SRR4302535.4308486/94‑1 (MQ=60)
TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                            >  SRR4302535.1398313/1‑94 (MQ=60)
TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCT                                                                                            <  SRR4302535.2915662/94‑1 (MQ=60)
  ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                          <  SRR4302535.866795/94‑1 (MQ=60)
  ACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGG                                                                                          <  SRR4302535.2250070/94‑1 (MQ=60)
   CCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGC                                                                                         >  SRR4302535.2088055/1‑94 (MQ=60)
    CGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCG                                                                                        >  SRR4302535.3824522/1‑94 (MQ=60)
    CGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCG                                                                                        >  SRR4302535.2330298/1‑94 (MQ=60)
     GGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGA                                                                                       >  SRR4302535.1036073/1‑94 (MQ=60)
     GGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGA                                                                                       >  SRR4302535.4639133/1‑94 (MQ=60)
       CGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGGCTTAAGCGTTGAAGCCCTGGCGATC                                                                                     >  SRR4302535.4424218/1‑94 (MQ=60)
        GGTTTCGCTTACGTTCTCGATGAGAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGGCTTAAGCGTTGACGCCCTGGCGATCC                                                                                    >  SRR4302535.4108034/1‑94 (MQ=60)
        GGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCC                                                                                    >  SRR4302535.1435225/1‑94 (MQ=60)
          TTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCAGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCAT                                                                                  <  SRR4302535.3320377/94‑1 (MQ=60)
           TTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATG                                                                                 <  SRR4302535.2330097/94‑1 (MQ=60)
             CGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAA                                                                               <  SRR4302535.2319205/94‑1 (MQ=60)
             CGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAA                                                                               <  SRR4302535.3537715/94‑1 (MQ=60)
              GCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAG                                                                              >  SRR4302535.1476410/1‑94 (MQ=60)
              GCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAG                                                                              >  SRR4302535.3631692/1‑94 (MQ=60)
              GCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAG                                                                              <  SRR4302535.1616223/94‑1 (MQ=60)
               CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGA                                                                             <  SRR4302535.1411107/94‑1 (MQ=60)
               CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGA                                                                             >  SRR4302535.1280304/1‑94 (MQ=60)
               CTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGA                                                                             >  SRR4302535.956970/1‑94 (MQ=60)
                TTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAATAG                                                                            >  SRR4302535.1784354/1‑94 (MQ=60)
                   CGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCAT                                                                         <  SRR4302535.3453437/94‑1 (MQ=60)
                     TTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCT                                                                       >  SRR4302535.1736903/1‑94 (MQ=60)
                     TTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCT                                                                       <  SRR4302535.164794/94‑1 (MQ=60)
                     TTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCT                                                                       <  SRR4302535.56490/94‑1 (MQ=60)
                        TCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCG                                                                    <  SRR4302535.3554566/94‑1 (MQ=60)
                        TCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCG                                                                    >  SRR4302535.2474186/1‑94 (MQ=60)
                          GATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTG                                                                  >  SRR4302535.1674493/1‑94 (MQ=60)
                           ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGTCGCCCTGGCGATCCATGAAGAGCATCTGCGTGT                                                                 >  SRR4302535.3979240/1‑94 (MQ=60)
                           ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                 >  SRR4302535.3277338/1‑94 (MQ=60)
                           ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                 >  SRR4302535.289971/1‑94 (MQ=60)
                           ATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGG                                                                 <  SRR4302535.1165472/94‑1 (MQ=60)
                                    GCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCAC                                                        >  SRR4302535.1548308/1‑94 (MQ=60)
                                     CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACTCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                       <  SRR4302535.699658/94‑1 (MQ=60)
                                     CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                       >  SRR4302535.140890/1‑94 (MQ=60)
                                     CGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACC                                                       <  SRR4302535.400291/94‑1 (MQ=60)
                                      GATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCG                                                      >  SRR4302535.488269/1‑94 (MQ=60)
                                       ATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGA                                                     <  SRR4302535.119526/94‑1 (MQ=60)
                                       ATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGA                                                     >  SRR4302535.2017172/1‑94 (MQ=60)
                                           CCGCAAACGCGTTAACCCGGAACTGGTCTAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAT                                                 <  SRR4302535.2495549/94‑1 (MQ=60)
                                           CCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAT                                                 >  SRR4302535.1453397/1‑94 (MQ=60)
                                           CCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAT                                                 >  SRR4302535.4251820/1‑94 (MQ=60)
                                           CCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCAT                                                 >  SRR4302535.3980679/1‑94 (MQ=60)
                                             GCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGT                                               <  SRR4302535.548124/94‑1 (MQ=60)
                                             GCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGT                                               <  SRR4302535.112494/94‑1 (MQ=60)
                                              CAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTG                                              >  SRR4302535.3038723/1‑94 (MQ=60)
                                              CAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGCG                                              >  SRR4302535.909734/1‑94 (MQ=60)
                                                AACGCGTTAACCCGGAACTGGTCGACGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCA                                            >  SRR4302535.3951616/1‑94 (MQ=60)
                                                  CGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGC                                          <  SRR4302535.860571/94‑1 (MQ=60)
                                                   GCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCA                                         >  SRR4302535.1638214/1‑94 (MQ=60)
                                                    CGTTACCCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCAT                                        <  SRR4302535.3971023/94‑1 (MQ=60)
                                                    CGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATATGCAGCAT                                        >  SRR4302535.2211413/1‑94 (MQ=60)
                                                      TTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATAC                                      <  SRR4302535.1413774/94‑1 (MQ=60)
                                                      TTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATAC                                      <  SRR4302535.3972595/94‑1 (MQ=60)
                                                        AACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCG                                    <  SRR4302535.3399204/94‑1 (MQ=60)
                                                          CCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGC                                  <  SRR4302535.2935025/94‑1 (MQ=60)
                                                          CCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGC                                  >  SRR4302535.1932562/1‑94 (MQ=60)
                                                           CCGGAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCT                                 >  SRR4302535.3170686/1‑94 (MQ=60)
                                                              GAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTC                              >  SRR4302535.4373196/1‑94 (MQ=60)
                                                              GAACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTC                              >  SRR4302535.2651024/1‑94 (MQ=60)
                                                               AACTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCA                             <  SRR4302535.719559/94‑1 (MQ=60)
                                                                 CTGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGC                           <  SRR4302535.3870907/94‑1 (MQ=60)
                                                                  TGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCG                          >  SRR4302535.3579203/1‑94 (MQ=60)
                                                                  TGGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCG                          <  SRR4302535.1609866/94‑1 (MQ=60)
                                                                   GGTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGC                         >  SRR4302535.426485/1‑94 (MQ=60)
                                                                    GTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCG                        <  SRR4302535.3659486/94‑1 (MQ=60)
                                                                    GTCGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCG                        >  SRR4302535.1650484/1‑94 (MQ=60)
                                                                      CGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGT                      <  SRR4302535.843434/94‑1 (MQ=60)
                                                                      CGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGT                      >  SRR4302535.327213/1‑94 (MQ=60)
                                                                      CGAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGT                      >  SRR4302535.3927210/1‑94 (MQ=60)
                                                                       GAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTG                     <  SRR4302535.1440886/94‑1 (MQ=60)
                                                                       GAGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTG                     <  SRR4302535.4606812/94‑1 (MQ=60)
                                                                        AGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGA                    <  SRR4302535.2700862/94‑1 (MQ=60)
                                                                        AGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGA                    >  SRR4302535.2580460/1‑94 (MQ=60)
                                                                        AGGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGA                    >  SRR4302535.1275929/1‑94 (MQ=60)
                                                                         GGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAA                   >  SRR4302535.1828377/1‑94 (MQ=60)
                                                                         GGTCTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTATCAGCGCGGTGAA                   >  SRR4302535.4619453/1‑94 (MQ=60)
                                                                            CTTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAG                >  SRR4302535.3409310/1‑94 (MQ=60)
                                                                             TTAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGA               >  SRR4302535.3332031/1‑94 (MQ=60)
                                                                              TAAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAT              <  SRR4302535.3536629/94‑1 (MQ=60)
                                                                              TAAGCGTGGTCGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGAT              <  SRR4302535.3077041/94‑1 (MQ=60)
                                                                               AAGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATT             >  SRR4302535.1949160/1‑94 (MQ=60)
                                                                                AGCGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTC            >  SRR4302535.1514178/1‑94 (MQ=60)
                                                                                  CGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTG          >  SRR4302535.4361464/1‑94 (MQ=60)
                                                                                  CGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTG          >  SRR4302535.1800698/1‑94 (MQ=60)
                                                                                  CGTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTG          >  SRR4302535.4234123/1‑94 (MQ=60)
                                                                                   GTTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGG         <  SRR4302535.1618538/94‑1 (MQ=60)
                                                                                   GTTGACGCCCTGGCGATCCATGAAGAACATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCTGTGAAGAGATTATGG         >  SRR4302535.2180043/1‑94 (MQ=60)
                                                                                    TTGACGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGC        <  SRR4302535.3660680/94‑1 (MQ=60)
                                                                                        CGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAAC    >  SRR4302535.2178908/1‑94 (MQ=60)
                                                                                        CGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAAC    <  SRR4302535.2313534/94‑1 (MQ=60)
                                                                                        CGCCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAAC    >  SRR4302535.1137875/1‑94 (MQ=60)
                                                                                          CCCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTG  <  SRR4302535.78193/94‑1 (MQ=60)
                                                                                           |                                                                                            
TGACCGGCGGTTTCGCTTACGTTCTCGATGAAAGCGGCGATTTCCGCAAACGCGTTAACCCGGAACTGGTCGAGGTCTTAAGCGTTGACGCTCTGGCGATCCATGAAGAGCATCTGCGTGGTCTTATCACCGAGCATGTGCAGCATACCGGCTCTCAGCGCGGTGAAGAGATTCTGGCGAACTG  >  NC_000913/3356525‑3356708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑