Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,370,778	A→G	V145A (GTA→GCA)	nanA ←	N‑acetylneuraminate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,370,778	0	A	G	99.5%	687.0 / NA	219	V145A (GTA→GCA)	nanA	N‑acetylneuraminate lyase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (113/105); minor base C (0/1); total (113/106)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

CCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCA  >  NC_000913/3370689‑3370867

 cATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGcccc                                                                                       >  1:1965891/1‑93 (MQ=255)

 cATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGcccc                                                                                       >  1:4431356/1‑93 (MQ=255)

  aTCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCa                                                                                      >  2:1066142/1‑93 (MQ=255)

     tGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTc                                                                                   >  1:1107641/1‑93 (MQ=255)

     tGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTc                                                                                   >  2:2922922/1‑93 (MQ=255)

      gATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCa                                                                                  >  1:4494802/1‑93 (MQ=255)

       aTAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAg                                                                                 <  2:69746/93‑1 (MQ=255)

       aTAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAg                                                                                 >  2:4638649/1‑93 (MQ=255)

        tAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAgg                                                                                >  2:3033761/1‑93 (MQ=255)

        tAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAgg                                                                                >  2:2457319/1‑93 (MQ=255)

        tAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAgg                                                                                >  2:1760013/1‑93 (MQ=255)

          gagaTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGAAATGTAACAAGTGGGTTGTTCTGATCCAGGGCCAGTTTTGCCCCACTCAgggt                                                                              >  1:4410253/1‑92 (MQ=255)

            gaTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTg                                                                            >  1:1165288/1‑93 (MQ=255)

            gaTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTg                                                                            >  1:2241465/1‑93 (MQ=255)

             aTCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTgg                                                                           >  2:2230212/1‑93 (MQ=255)

               cGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGaa                                                                         <  1:815990/93‑1 (MQ=255)

               cGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGaa                                                                         <  1:169029/93‑1 (MQ=255)

                  cAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgt                                                                      <  2:1217196/93‑1 (MQ=255)

                   agagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgtt                                                                     >  2:1980710/1‑93 (MQ=255)

                   agagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgtt                                                                     <  1:2102407/93‑1 (MQ=255)

                   agagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgtt                                                                     <  2:3594341/93‑1 (MQ=255)

                    gagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgttg                                                                    >  1:3197295/1‑93 (MQ=255)

                    gagGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGGCAGTTTTCTTCCACTCAGGGCTGGAAtgttg                                                                    >  2:4302338/1‑93 (MQ=255)

                     agGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAtgttgt                                                                   <  1:3857545/93‑1 (MQ=255)

                      ggTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTa                                                                  <  1:977078/93‑1 (MQ=255)

                        tCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTaca                                                                >  1:1029213/1‑93 (MQ=255)

                          tGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAcacc                                                              >  1:3814246/1‑93 (MQ=255)

                            tttCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAcaccac                                                            <  2:599960/93‑1 (MQ=255)

                            tttCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAcaccac                                                            >  2:2624876/1‑93 (MQ=255)

                              tCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACAccacca                                                          >  1:4126341/1‑93 (MQ=255)

                               cAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCAt                                                         <  1:2706124/93‑1 (MQ=255)

                               cAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCAt                                                         <  1:695207/93‑1 (MQ=255)

                                aGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATc                                                        <  1:2494030/93‑1 (MQ=255)

                                 gcgcACCTCCGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCg                                                       <  1:1794500/93‑1 (MQ=255)

                                  cgcACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCgg                                                      >  1:2456663/1‑93 (MQ=255)

                                      ccTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCTGCaaa                                                  >  1:779040/1‑93 (MQ=255)

                                      ccTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCaaa                                                  >  1:880008/1‑93 (MQ=255)

                                      ccTACGCCAGGCAATGTAACAAGAGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCaaa                                                  >  2:4681578/1‑93 (MQ=255)

                                       cTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAAc                                                 <  2:3253770/93‑1 (MQ=255)

                                          cGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCAt                                              <  2:2211361/93‑1 (MQ=255)

                                           gCCAGGCANTGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATc                                             <  2:4396709/93‑1 (MQ=255)

                                            ccAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATcc                                            >  2:3566007/1‑93 (MQ=255)

                                             cAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccg                                           >  1:39032/1‑93 (MQ=255)

                                             cAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccg                                           >  2:572787/1‑93 (MQ=255)

                                              aGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgc                                          >  1:2550139/1‑93 (MQ=255)

                                                gCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgccg                                        >  1:259059/1‑93 (MQ=255)

                                                  aaTGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa                                      <  1:437452/93‑1 (MQ=255)

                                                  aaTGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa                                      >  1:564374/1‑93 (MQ=255)

                                                   aTGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAAt                                     <  1:2662851/93‑1 (MQ=255)

                                                    tGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATc                                    <  2:3121265/93‑1 (MQ=255)

                                                    tGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATc                                    <  1:1300971/93‑1 (MQ=255)

                                                    tGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATc                                    >  1:144790/1‑93 (MQ=255)

                                                     gTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCa                                   >  1:2738294/1‑93 (MQ=255)

                                                     gTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCa                                   >  1:3360638/1‑93 (MQ=255)

                                                      tAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaa                                  >  2:4239456/1‑93 (MQ=255)

                                                      tAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaa                                  <  2:1562407/93‑1 (MQ=255)

                                                        aCAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaata                                <  2:36804/93‑1 (MQ=255)

                                                        aCAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaata                                >  2:3705563/1‑93 (MQ=255)

                                                        aCAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaata                                <  1:2192771/93‑1 (MQ=255)

                                                        aCAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaata                                >  1:2001325/1‑93 (MQ=255)

                                                         cAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaataa                               >  1:888724/1‑93 (MQ=255)

                                                         cAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaataa                               >  2:62594/1‑93 (MQ=255)

                                                          aaGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaataat                              <  2:385455/93‑1 (MQ=255)

                                                          aaGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaataat                              >  1:3072141/1‑93 (MQ=255)

                                                           aGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAtt                             <  2:4039797/93‑1 (MQ=255)

                                                           aGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAtt                             >  2:2295117/1‑93 (MQ=255)

                                                           aGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAtt                             <  2:2324625/93‑1 (MQ=255)

                                                           aGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAtt                             <  1:1627914/93‑1 (MQ=255)

                                                           aGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAtt                             <  2:4541650/93‑1 (MQ=255)

                                                            gtgtgtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTg                            >  1:1848990/1‑93 (MQ=255)

                                                             tgtgtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGc                           >  2:2847/1‑93 (MQ=255)

                                                              gtgtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGcc                          <  1:1557569/93‑1 (MQ=255)

                                                               tgtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGccc                         <  1:415942/93‑1 (MQ=255)

                                                               tgtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGccc                         <  2:3451466/93‑1 (MQ=255)

                                                                gtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg                        >  2:2783055/1‑93 (MQ=255)

                                                                gtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg                        >  1:4196129/1‑93 (MQ=255)

                                                                gtTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg                        >  1:3299506/1‑93 (MQ=255)

                                                                   gatctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATTTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATa                     >  1:2516589/1‑93 (MQ=255)

                                                                   gatctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATa                     <  2:1459865/93‑1 (MQ=255)

                                                                     tctgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGt                   <  2:2047311/93‑1 (MQ=255)

                                                                      ctaatcCAGGTTCAGTTTTCCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg                  <  2:2575961/90‑1 (MQ=255)

                                                                       tgatcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGa                 <  1:3663988/93‑1 (MQ=255)

                                                                          tcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCg              >  2:81872/1‑93 (MQ=255)

                                                                          tcCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCg              <  1:2416109/93‑1 (MQ=255)

                                                                           ccAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACGATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGc             >  2:3782437/1‑93 (MQ=255)

                                                                           ccAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGc             >  1:1209263/1‑93 (MQ=255)

                                                                           ccAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGc             >  1:1902474/1‑93 (MQ=255)

                                                                            cAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCa            <  1:2115401/93‑1 (MQ=255)

                                                                              gggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt          >  2:2370341/1‑93 (MQ=255)

                                                                              gggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt          >  2:1899525/1‑93 (MQ=255)

                                                                              gggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt          >  1:4285144/1‑93 (MQ=255)

                                                                              gggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt          >  1:2909136/1‑93 (MQ=255)

                                                                              gggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgt          >  2:882591/1‑93 (MQ=255)

                                                                               ggTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgtg         <  1:3436916/93‑1 (MQ=255)

                                                                                gTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAgtgt        >  2:3529959/1‑93 (MQ=255)

                                                                                    gTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGttctt    >  2:4165509/1‑93 (MQ=255)

                                                                                    gTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGttctt    >  1:3573334/1‑93 (MQ=255)

                                                                                    gTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGttctt    <  2:800643/93‑1 (MQ=255)

                                                                                     ttttGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGttcttc   <  2:2505847/93‑1 (MQ=255)

                                                                                      tttGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCa  >  1:3786874/1‑93 (MQ=255)

CCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCA  >  NC_000913/3370689‑3370867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAG  >  NC_000913/3370685‑3370870

TGCTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTG                                                                                              >  SRR4302535.2447770/1‑94 (MQ=60)

    CCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCC                                                                                          >  SRR4302535.4461719/1‑94 (MQ=60)

    CCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCC                                                                                          >  SRR4302535.1980062/1‑94 (MQ=60)

     CATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCA                                                                                         >  SRR4302535.1269773/1‑94 (MQ=60)

     CATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCA                                                                                         <  SRR4302535.37928/94‑1 (MQ=60)

     CATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCA                                                                                         >  SRR4302535.1636664/1‑94 (MQ=60)

        CTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTC                                                                                      >  SRR4302535.1115553/1‑94 (MQ=60)

         TGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCA                                                                                     <  SRR4302535.2825222/94‑1 (MQ=60)

         TGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCA                                                                                     >  SRR4302535.797519/1‑94 (MQ=60)

         TGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCA                                                                                     >  SRR4302535.4525655/1‑94 (MQ=60)

          GATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAG                                                                                    <  SRR4302535.4421830/94‑1 (MQ=60)

           ATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGG                                                                                   <  SRR4302535.1957031/94‑1 (MQ=60)

            TAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGG                                                                                  <  SRR4302535.3287110/94‑1 (MQ=60)

             AGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGC                                                                                 >  SRR4302535.217877/1‑94 (MQ=60)

             AGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGC                                                                                 >  SRR4302535.3488247/1‑94 (MQ=60)

             AGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGAAATGTAACAAGTGGGTTGTTCTGATCCAGGGCCAGTTTTGCCCCACTCAGGGT                                                                                 >  SRR4302535.4440434/1‑94 (MQ=60)

               AGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTG                                                                               >  SRR4302535.2257410/1‑94 (MQ=60)

               AGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTG                                                                               >  SRR4302535.1173611/1‑94 (MQ=60)

                   CGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAT                                                                           <  SRR4302535.821711/94‑1 (MQ=60)

                   CGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAT                                                                           <  SRR4302535.170203/94‑1 (MQ=60)

                   CGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAAT                                                                           <  SRR4302535.707761/94‑1 (MQ=60)

                    GCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATG                                                                          <  SRR4302535.4475698/94‑1 (MQ=60)

                     CCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGCCAGTTTTGCCCCACTCAGGGCTGGAATGT                                                                         >  SRR4302535.987041/1‑94 (MQ=60)

                      CAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTT                                                                        >  SRR4302535.383537/1‑94 (MQ=60)

                       AGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTG                                                                       <  SRR4302535.2701907/94‑1 (MQ=60)

                       AGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTG                                                                       >  SRR4302535.3219420/1‑94 (MQ=60)

                       AGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTG                                                                       <  SRR4302535.2117570/94‑1 (MQ=60)

                         AGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTA                                                                     <  SRR4302535.3884100/94‑1 (MQ=60)

                          GGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAC                                                                    <  SRR4302535.984015/94‑1 (MQ=60)

                          GGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTAC                                                                    >  SRR4302535.3793566/1‑94 (MQ=60)

                           GTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACA                                                                   >  SRR4302535.2628464/1‑94 (MQ=60)

                           GTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACA                                                                   >  SRR4302535.1036520/1‑94 (MQ=60)

                            TCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACAC                                                                  <  SRR4302535.1918381/94‑1 (MQ=60)

                             CTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACC                                                                 >  SRR4302535.3840564/1‑94 (MQ=60)

                                 TTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCA                                                             >  SRR4302535.4154577/1‑94 (MQ=60)

                                   CAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATC                                                           <  SRR4302535.700085/94‑1 (MQ=60)

                                   CAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATC                                                           <  SRR4302535.2725462/94‑1 (MQ=60)

                                    AGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCG                                                          <  SRR4302535.2512026/94‑1 (MQ=60)

                                     GCGCACCTCCGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGG                                                         <  SRR4302535.1807473/94‑1 (MQ=60)

                                     GCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGG                                                         >  SRR4302535.2474378/1‑94 (MQ=60)

                                      CGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGC                                                        <  SRR4302535.3914092/94‑1 (MQ=60)

                                      CGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGC                                                        <  SRR4302535.4143612/94‑1 (MQ=60)

                                       GCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCA                                                       >  SRR4302535.4197122/1‑94 (MQ=60)

                                       GCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCA                                                       >  SRR4302535.3458471/1‑94 (MQ=60)

                                        CACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAA                                                      >  SRR4302535.4704634/1‑94 (MQ=60)

                                         ACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCTGCAAA                                                     >  SRR4302535.784511/1‑94 (MQ=60)

                                         ACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAA                                                     >  SRR4302535.886239/1‑94 (MQ=60)

                                             ACGCCGGGCAATGTAACAAGTGTGTTGATCTGATGCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACCCCACCATCGGCAAACCAT                                                 <  SRR4302535.2124210/94‑1 (MQ=60)

                                             ACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCAT                                                 <  SRR4302535.2233428/94‑1 (MQ=60)

                                              CGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATC                                                <  SRR4302535.1289495/94‑1 (MQ=60)

                                               GCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCC                                               <  SRR4302535.1772238/94‑1 (MQ=60)

                                               GCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCC                                               >  SRR4302535.802146/1‑94 (MQ=60)

                                               GCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCC                                               <  SRR4302535.1836896/94‑1 (MQ=60)

                                                CCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCG                                              >  SRR4302535.39332/1‑94 (MQ=60)

                                                CCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCG                                              <  SRR4302535.2508481/94‑1 (MQ=60)

                                                 CAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGC                                             >  SRR4302535.2568496/1‑94 (MQ=60)

                                                  AGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCC                                            >  SRR4302535.1688295/1‑94 (MQ=60)

                                                   GGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCG                                           >  SRR4302535.260850/1‑94 (MQ=60)

                                                     CAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAA                                         >  SRR4302535.568326/1‑94 (MQ=60)

                                                     CAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAA                                         >  SRR4302535.4317099/1‑94 (MQ=60)

                                                      AATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAAT                                        <  SRR4302535.2751090/94‑1 (MQ=60)

                                                      AATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAAT                                        <  SRR4302535.440510/94‑1 (MQ=60)

                                                       ATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATC                                       >  SRR4302535.145801/1‑94 (MQ=60)

                                                       ATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATC                                       <  SRR4302535.2681920/94‑1 (MQ=60)

                                                        TGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCA                                      >  SRR4302535.2757822/1‑94 (MQ=60)

                                                        TGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCA                                      <  SRR4302535.1310249/94‑1 (MQ=60)

                                                        TGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCA                                      >  SRR4302535.3384029/1‑94 (MQ=60)

                                                           AACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATA                                   >  SRR4302535.4588888/1‑94 (MQ=60)

                                                           AACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATA                                   <  SRR4302535.4164704/94‑1 (MQ=60)

                                                           AACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATA                                   <  SRR4302535.2811805/94‑1 (MQ=60)

                                                           AACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATA                                   >  SRR4302535.2015744/1‑94 (MQ=60)

                                                            ACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAA                                  >  SRR4302535.2163007/1‑94 (MQ=60)

                                                            ACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAA                                  >  SRR4302535.895019/1‑94 (MQ=60)

                                                            ACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAA                                  >  SRR4302535.1362706/1‑94 (MQ=60)

                                                            ACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAA                                  <  SRR4302535.2208398/94‑1 (MQ=60)

                                                             CAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAT                                 >  SRR4302535.3093590/1‑94 (MQ=60)

                                                             CAAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAT                                 >  SRR4302535.2475641/1‑94 (MQ=60)

                                                              AAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATT                                >  SRR4302535.4461528/1‑94 (MQ=60)

                                                              AAGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATT                                >  SRR4302535.3863531/1‑94 (MQ=60)

                                                               AGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTG                               <  SRR4302535.1639666/94‑1 (MQ=60)

                                                               AGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTG                               >  SRR4302535.1862314/1‑94 (MQ=60)

                                                               AGTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTG                               <  SRR4302535.2558212/94‑1 (MQ=60)

                                                                GTGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGC                              <  SRR4302535.4003061/94‑1 (MQ=60)

                                                                 TGTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTAGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCC                             <  SRR4302535.3324382/94‑1 (MQ=60)

                                                                  GTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCC                            <  SRR4302535.1568797/94‑1 (MQ=60)

                                                                  GTGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCC                            <  SRR4302535.1490910/94‑1 (MQ=60)

                                                                   TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                           >  SRR4302535.4224845/1‑94 (MQ=60)

                                                                   TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                           <  SRR4302535.418857/94‑1 (MQ=60)

                                                                   TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                           >  SRR4302535.3322452/1‑94 (MQ=60)

                                                                   TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                           <  SRR4302535.841702/94‑1 (MQ=60)

                                                                   TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                           >  SRR4302535.2522842/1‑94 (MQ=60)

                                                                   TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                           >  SRR4302535.2640336/1‑94 (MQ=60)

                                                                   TGTTGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCG                           <  SRR4302535.3174317/94‑1 (MQ=60)

                                                                      TGATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATTTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATA                        >  SRR4302535.2534727/1‑94 (MQ=60)

                                                                       GATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAG                       <  SRR4302535.1182375/94‑1 (MQ=60)

                                                                        ATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGT                      >  SRR4302535.333224/1‑94 (MQ=60)

                                                                        ATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGT                      >  SRR4302535.2257544/1‑94 (MQ=60)

                                                                        ATCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGT                      <  SRR4302535.929848/94‑1 (MQ=60)

                                                                         TCTGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTG                     <  SRR4302535.1161996/94‑1 (MQ=60)

                                                                           TGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGAT                   <  SRR4302535.3689352/94‑1 (MQ=60)

                                                                           TGATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGAT                   <  SRR4302535.1995361/94‑1 (MQ=60)

                                                                            GATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATC                  <  SRR4302535.2591422/94‑1 (MQ=60)

                                                                             ATCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCG                 >  SRR4302535.2454880/1‑94 (MQ=60)

                                                                              TCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGC                >  SRR4302535.1916166/1‑94 (MQ=60)

                                                                              TCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGC                <  SRR4302535.2433500/94‑1 (MQ=60)

                                                                              TCCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGC                >  SRR4302535.1217951/1‑94 (MQ=60)

                                                                               CCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCA               >  SRR4302535.639196/1‑94 (MQ=60)

                                                                               CCAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCA               <  SRR4302535.4240410/94‑1 (MQ=60)

                                                                                CAGGTTCAGGTTTGCCCCGCTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAG              <  SRR4302535.2328021/94‑1 (MQ=60)

                                                                                CAGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAG              <  SRR4302535.2130669/94‑1 (MQ=60)

                                                                                 AGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGT             <  SRR4302535.4337831/94‑1 (MQ=60)

                                                                                 AGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGT             >  SRR4302535.4314459/1‑94 (MQ=60)

                                                                                 AGGGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGT             >  SRR4302535.2929694/1‑94 (MQ=60)

                                                                                   GGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAACCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT           <  SRR4302535.3460821/94‑1 (MQ=60)

                                                                                   GGTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT           <  SRR4302535.887800/94‑1 (MQ=60)

                                                                                    GTCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTT          >  SRR4302535.108396/1‑94 (MQ=60)

                                                                                     TCAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTC         <  SRR4302535.2995090/94‑1 (MQ=60)

                                                                                      CAGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCT        <  SRR4302535.1888924/94‑1 (MQ=60)

                                                                                       AGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTT       >  SRR4302535.3598112/1‑94 (MQ=60)

                                                                                       AGTTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTT       <  SRR4302535.2046055/94‑1 (MQ=60)

                                                                                        GTTTTGCCCCACTCAGGGCTGGAATGTTGTGCACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTC      <  SRR4302535.3631616/94‑1 (MQ=60)

                                                                                         TTTTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCA     >  SRR4302535.3812996/1‑94 (MQ=60)

                                                                                          TTTGCCCCACGCAGGGCTGCAATGTTGTCCACCACCATAGGCAAACCATCCGCAGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAA    <  SRR4302535.1662215/94‑1 (MQ=60)

                                                                                           TTGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAA   <  SRR4302535.3477981/94‑1 (MQ=60)

                                                                                            TGCCCCACTCAGGGCTGTAATGTTGTACGCCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAG  <  SRR4302535.4539291/94‑1 (MQ=60)

                                                                                            TGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAG  <  SRR4302535.4618400/94‑1 (MQ=60)

                                                                                            TGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAG  >  SRR4302535.784700/1‑94 (MQ=60)

                                                                                            TGCCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAG  <  SRR4302535.4438575/94‑1 (MQ=60)

TGCTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAG  >  NC_000913/3370685‑3370870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`