Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,525,330	T→C	T408T (ACT→ACC)	yrfF →	putative dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,525,330	0	T	C	99.6%	739.1 / ‑5.0	238	T408T (ACT→ACC)	yrfF	putative dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (110/127); total (111/127)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.68e-01

Only 100 of 247 total aligned reads displayed.

AGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATT  >  NC_000913/3525240‑3525419

    accaGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGa                                                                                     >  1:407438/1‑93 (MQ=255)

       aGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAAcc                                                                                  >  1:292579/1‑93 (MQ=255)

         cGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTg                                                                                <  2:1566914/93‑1 (MQ=255)

          gTAAAACAACTGGCTGACGCTGTCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTgg                                                                               <  1:4545835/93‑1 (MQ=255)

           tAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGa                                                                              <  2:4560270/93‑1 (MQ=255)

           tAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGa                                                                              <  2:2515536/93‑1 (MQ=255)

             aaaCAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAgc                                                                            <  1:1710319/93‑1 (MQ=255)

               acaacTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAgcgc                                                                          >  1:3277833/1‑93 (MQ=255)

                 agcTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGa                                                                        <  1:1739787/91‑1 (MQ=255)

                 aacTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGa                                                                        >  1:4604380/1‑93 (MQ=255)

                 aacTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGa                                                                        >  1:2218199/1‑93 (MQ=255)

                 aacTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAAACTCCGGAACCTGGAGCGCGa                                                                        >  1:2321387/1‑93 (MQ=255)

                  acTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGaa                                                                       <  2:2423781/93‑1 (MQ=255)

                    tGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGaaaa                                                                     <  2:466240/93‑1 (MQ=255)

                    tGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGaaaa                                                                     <  2:3813189/93‑1 (MQ=255)

                     ggCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAAc                                                                    >  2:4094987/1‑93 (MQ=255)

                     ggCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAAc                                                                    <  1:4478631/93‑1 (MQ=255)

                        tGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCaa                                                                 >  1:2087430/1‑93 (MQ=255)

                         gACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAAt                                                                >  2:3737424/1‑93 (MQ=255)

                          aCGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAAtt                                                               >  1:2958920/1‑93 (MQ=255)

                             cTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCAc                                                            >  1:1428442/1‑93 (MQ=255)

                             cTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCAc                                                            >  2:247351/1‑93 (MQ=255)

                              tGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGACCCTCCGGAACCTGGAGCGCGAAAACCAATTCAcc                                                           <  2:2968562/93‑1 (MQ=255)

                                gCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCtt                                                         <  2:3906018/93‑1 (MQ=255)

                                 cGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCttt                                                        <  2:3064518/93‑1 (MQ=255)

                                  gggCGGGTAGGCCATACATTGCGTAGTAGCGGTACGGGAATGTGTAATATTCGACCCTCGGGAACCTGGAGCGCGAAAACCAATTCCCCtttt                                                       <  2:3272608/91‑1 (MQ=255)

                                  gTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCtttt                                                       <  2:1178989/93‑1 (MQ=255)

                                   tGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGGGCGAAAACCACTTCACCttttt                                                      >  1:2604126/1‑93 (MQ=255)

                                   tGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCttttt                                                      <  1:495790/93‑1 (MQ=255)

                                   tGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCttttt                                                      <  2:2013997/93‑1 (MQ=255)

                                   tGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCttttt                                                      <  1:1049847/93‑1 (MQ=255)

                                   tGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCttttt                                                      <  1:2040794/93‑1 (MQ=255)

                                     cGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTa                                                    <  1:3483380/93‑1 (MQ=255)

                                      gggTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTAc                                                   <  1:3553362/93‑1 (MQ=255)

                                      gggTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTAc                                                   >  2:2355301/1‑93 (MQ=255)

                                       ggTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTAcc                                                  >  1:4247200/1‑93 (MQ=255)

                                         tAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGt                                                >  2:2862087/1‑93 (MQ=255)

                                          aGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGtt                                               <  2:4486754/93‑1 (MQ=255)

                                          aGGCGATACATTGCGTATTAGCGGTACGGGAATGAGCAATATTCGAACCTCCGGACCCTGGCGCGCGAAAACCAATTCACCTTTTTTAGCGtt                                               >  2:1618916/1‑93 (MQ=255)

                                           ggCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGttt                                              <  2:1483177/93‑1 (MQ=255)

                                           ggCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGttt                                              <  1:999777/93‑1 (MQ=255)

                                           ggCGATACATTGCGTAGTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGttt                                              <  1:2372324/93‑1 (MQ=255)

                                             cGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGa                                            <  2:4214560/93‑1 (MQ=255)

                                              gATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGATCCTCCGGAACCTGGAGCGCGAAAACCAATTCCCCTTTTTTACCGTTTGAc                                           <  2:3635745/93‑1 (MQ=255)

                                              gATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGAc                                           >  2:3947150/1‑93 (MQ=255)

                                               aTACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACt                                          <  2:2758298/93‑1 (MQ=255)

                                               aTACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACt                                          >  2:2324805/1‑93 (MQ=255)

                                               aTACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACt                                          >  2:1233181/1‑93 (MQ=255)

                                               aTACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACt                                          >  2:2553750/1‑93 (MQ=255)

                                                tACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTg                                         <  2:2745696/93‑1 (MQ=255)

                                                tACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTg                                         >  1:3462382/1‑93 (MQ=255)

                                                tACATTGCGTATTAGCGGTACGGGAATCTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTg                                         >  2:913027/1‑93 (MQ=255)

                                                   aTTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTc                                      >  2:4350973/1‑93 (MQ=255)

                                                   aTTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTc                                      >  2:2910645/1‑93 (MQ=255)

                                                   aTTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTc                                      >  2:1836736/1‑93 (MQ=255)

                                                   aTTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTCTTTACCGTTTGACTGCTc                                      >  2:1961111/1‑93 (MQ=255)

                                                      gCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCa                                   >  2:1288928/1‑93 (MQ=255)

                                                       cGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAg                                  >  1:1365441/1‑93 (MQ=255)

                                                        gTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGa                                 >  1:2812768/1‑93 (MQ=255)

                                                        gTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGa                                 >  1:3839024/1‑93 (MQ=255)

                                                         tATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGat                                <  2:1916075/93‑1 (MQ=255)

                                                         tATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGat                                >  2:1390589/1‑93 (MQ=255)

                                                         tATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGat                                >  1:755149/1‑93 (MQ=255)

                                                          aTTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatc                               <  1:2143903/93‑1 (MQ=255)

                                                          aTTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatc                               <  2:3354922/93‑1 (MQ=255)

                                                           ttcgCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatca                              <  2:3290105/90‑1 (MQ=255)

                                                           ttAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatca                              >  1:213342/1‑93 (MQ=255)

                                                           ttAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatca                              <  1:1289368/93‑1 (MQ=255)

                                                           ttAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatca                              <  2:2116739/93‑1 (MQ=255)

                                                            tAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGGACCTGGAGCGCGAAAACCAATTCAAATTTTTTACCGTTTGACTGCTCGCAGatcat                             >  2:3155925/1‑93 (MQ=255)

                                                            tAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatcat                             >  1:4350028/1‑93 (MQ=255)

                                                            tAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatcat                             >  1:4090249/1‑93 (MQ=255)

                                                              gCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCt                           >  1:623308/1‑93 (MQ=255)

                                                              gCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCt                           <  2:626119/93‑1 (MQ=255)

                                                              gCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCt                           <  1:2100916/93‑1 (MQ=255)

                                                              gCGGTACGGGAATGTGTAATATTCGAACCCCCGGAACCTGGAGCCAGAACACCAATTCACCTTTTTTACCGCTTGACTGCTCGCAGATCATCt                           >  2:690571/1‑93 (MQ=255)

                                                               cGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTg                          <  1:1886676/93‑1 (MQ=255)

                                                                  tACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGaa                       >  2:501590/1‑93 (MQ=255)

                                                                  tACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGaa                       <  2:4433840/93‑1 (MQ=255)

                                                                   aCGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAac                      <  1:552885/93‑1 (MQ=255)

                                                                   aCGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAac                      <  2:188897/93‑1 (MQ=255)

                                                                   aCGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAac                      >  2:2083763/1‑93 (MQ=255)

                                                                    cGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAacg                     <  1:929667/93‑1 (MQ=255)

                                                                     gggAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAacga                    <  1:1440447/93‑1 (MQ=255)

                                                                      ggAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAacgac                   <  2:541700/93‑1 (MQ=255)

                                                                       gAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAacgacg                  <  2:1077479/93‑1 (MQ=255)

                                                                        aaTGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTATCGTTTGACTGCTCGCAGATCATCTGGAACGACGc                 >  2:1684216/1‑93 (MQ=255)

                                                                         aTGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGcc                >  1:834816/1‑93 (MQ=255)

                                                                           gtgtAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCg              <  1:1610099/93‑1 (MQ=255)

                                                                             gtAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCt            >  1:646542/1‑93 (MQ=255)

                                                                               aaTATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCa          >  1:3422560/1‑93 (MQ=255)

                                                                               aaTATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGAAGCCCGCTCa          >  2:83701/1‑93 (MQ=255)

                                                                                 tatTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCAtt        <  1:2618953/93‑1 (MQ=255)

                                                                                   ttCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGc      <  1:1008064/93‑1 (MQ=255)

                                                                                   ttCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGc      <  1:1165994/93‑1 (MQ=255)

                                                                                     cGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCa    >  2:43916/1‑93 (MQ=255)

                                                                                      gAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCAt   >  2:1262236/1‑93 (MQ=255)

                                                                                      gAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCAt   >  2:2230307/1‑93 (MQ=255)

                                                                                      gAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCAt   <  1:1521525/93‑1 (MQ=255)

                                                                                       aaCCTCCGGAATCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCAtt  <  1:287858/93‑1 (MQ=255)

AGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATT  >  NC_000913/3525240‑3525419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCA  >  NC_000913/3525237‑3525423

TTAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACC                                                                                               <  SRR4302535.3942276/94‑1 (MQ=60)

TGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACC                                                                                               >  SRR4302535.275770/1‑94 (MQ=60)

TGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACC                                                                                               <  SRR4302535.188556/94‑1 (MQ=60)

TGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACC                                                                                               >  SRR4302535.51872/1‑94 (MQ=60)

TGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACC                                                                                               >  SRR4302535.1998934/1‑94 (MQ=60)

TGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACC                                                                                               <  SRR4302535.1312922/94‑1 (MQ=60)

 GAAGCCACCAGCGTAAAAGAGCTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCT                                                                                              <  SRR4302535.76821/94‑1 (MQ=60)

  AAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTC                                                                                             <  SRR4302535.1113803/94‑1 (MQ=60)

   AGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCC                                                                                            >  SRR4302535.4556725/1‑94 (MQ=60)

   AGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCC                                                                                            <  SRR4302535.346641/94‑1 (MQ=60)

      CACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGA                                                                                         >  SRR4302535.441564/1‑94 (MQ=60)

      CACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGA                                                                                         >  SRR4302535.410293/1‑94 (MQ=60)

      CACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGA                                                                                         >  SRR4302535.1920828/1‑94 (MQ=60)

       ACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAA                                                                                        <  SRR4302535.2627174/94‑1 (MQ=60)

         CAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACC                                                                                      >  SRR4302535.294626/1‑94 (MQ=60)

          AGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCT                                                                                     >  SRR4302535.1703345/1‑94 (MQ=60)

           GCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGTTACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTG                                                                                    <  SRR4302535.2575975/94‑1 (MQ=60)

           GCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTG                                                                                    <  SRR4302535.3558532/94‑1 (MQ=60)

            CGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGG                                                                                   <  SRR4302535.4311335/94‑1 (MQ=60)

             GTAAAACAACTGGCTGACGCTGTCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGA                                                                                  <  SRR4302535.4576919/94‑1 (MQ=60)

               AAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGCAATGTGTAATATTCGAACCTCCGGAAGCTGGAGC                                                                                >  SRR4302535.1467395/1‑94 (MQ=60)

                AAACAGCTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCG                                                                               <  SRR4302535.681011/94‑1 (MQ=60)

                AAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCG                                                                               <  SRR4302535.1722683/94‑1 (MQ=60)

                 AACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGC                                                                              >  SRR4302535.3300602/1‑94 (MQ=60)

                 AACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGC                                                                              >  SRR4302535.2901432/1‑94 (MQ=60)

                 AACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGC                                                                              >  SRR4302535.924056/1‑94 (MQ=60)

                 AACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGC                                                                              >  SRR4302535.644245/1‑94 (MQ=60)

                   CAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGCATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGA                                                                            >  SRR4302535.2305735/1‑94 (MQ=60)

                   CAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGA                                                                            >  SRR4302535.2233984/1‑94 (MQ=60)

                   CAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGA                                                                            >  SRR4302535.4635682/1‑94 (MQ=60)

                   CAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAAACTCCGGAACCTGGAGCGCGA                                                                            >  SRR4302535.2338001/1‑94 (MQ=60)

                    AGCTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAA                                                                           <  SRR4302535.1752359/94‑1 (MQ=60)

                    AACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAA                                                                           >  SRR4302535.596102/1‑94 (MQ=60)

                    AACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAA                                                                           >  SRR4302535.4585417/1‑94 (MQ=60)

                     ACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAA                                                                          >  SRR4302535.2068423/1‑94 (MQ=60)

                      CTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAA                                                                         <  SRR4302535.4517495/94‑1 (MQ=60)

                       TGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAAC                                                                        <  SRR4302535.1439794/94‑1 (MQ=60)

                       TGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAAC                                                                        <  SRR4302535.1392281/94‑1 (MQ=60)

                        GGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACC                                                                       <  SRR4302535.4509379/94‑1 (MQ=60)

                         GCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTCCGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCA                                                                      <  SRR4302535.1646689/94‑1 (MQ=60)

                          CTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAA                                                                     >  SRR4302535.2102462/1‑94 (MQ=60)

                            GACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATT                                                                   >  SRR4302535.2979700/1‑94 (MQ=60)

                               GCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCAC                                                                >  SRR4302535.1438635/1‑94 (MQ=60)

                               GCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCAC                                                                <  SRR4302535.4275548/94‑1 (MQ=60)

                                CTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACC                                                               >  SRR4302535.296723/1‑94 (MQ=60)

                                 TGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCT                                                              <  SRR4302535.470295/94‑1 (MQ=60)

                                    CGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTT                                                           >  SRR4302535.2801912/1‑94 (MQ=60)

                                     GTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGGGCGAAAACCACTTCACCTTTTT                                                          >  SRR4302535.2622821/1‑94 (MQ=60)

                                     GTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTT                                                          <  SRR4302535.3011781/94‑1 (MQ=60)

                                     GTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTT                                                          <  SRR4302535.2410537/94‑1 (MQ=60)

                                      TGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTT                                                         <  SRR4302535.499272/94‑1 (MQ=60)

                                      TGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTT                                                         <  SRR4302535.2055492/94‑1 (MQ=60)

                                      TGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTT                                                         <  SRR4302535.1057314/94‑1 (MQ=60)

                                       GCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTA                                                        <  SRR4302535.4030547/94‑1 (MQ=60)

                                        CGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTAC                                                       <  SRR4302535.3507567/94‑1 (MQ=60)

                                         GGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACC                                                      <  SRR4302535.2157420/94‑1 (MQ=60)

                                         GGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACC                                                      >  SRR4302535.4276288/1‑94 (MQ=60)

                                         GGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACC                                                      >  SRR4302535.2933343/1‑94 (MQ=60)

                                         GGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACC                                                      <  SRR4302535.3578019/94‑1 (MQ=60)

                                           GTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGT                                                    >  SRR4302535.1048190/1‑94 (MQ=60)

                                             AGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTT                                                  >  SRR4302535.4664748/1‑94 (MQ=60)

                                              GGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTG                                                 <  SRR4302535.1006885/94‑1 (MQ=60)

                                              GGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTG                                                 >  SRR4302535.1734124/1‑94 (MQ=60)

                                              GGCGATACATTGCGTAGTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTG                                                 <  SRR4302535.2389365/94‑1 (MQ=60)

                                                 GATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACT                                              >  SRR4302535.808039/1‑94 (MQ=60)

                                                  ATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTG                                             >  SRR4302535.2112277/1‑94 (MQ=60)

                                                  ATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTG                                             >  SRR4302535.3486451/1‑94 (MQ=60)

                                                      ATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCG                                         <  SRR4302535.1005259/94‑1 (MQ=60)

                                                      ATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCG                                         >  SRR4302535.1487752/1‑94 (MQ=60)

                                                      ATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCG                                         <  SRR4302535.3731828/94‑1 (MQ=60)

                                                         GCGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAG                                      >  SRR4302535.1375204/1‑94 (MQ=60)

                                                          CGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGA                                     >  SRR4302535.3865485/1‑94 (MQ=60)

                                                          CGTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGA                                     >  SRR4302535.2832756/1‑94 (MQ=60)

                                                          CGTATTAGCGATACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGA                                     <  SRR4302535.2095012/94‑1 (MQ=60)

                                                           GTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGAT                                    <  SRR4302535.1599476/94‑1 (MQ=60)

                                                           GTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGAT                                    >  SRR4302535.1310026/1‑94 (MQ=60)

                                                           GTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGAT                                    <  SRR4302535.4003898/94‑1 (MQ=60)

                                                           GTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGAT                                    >  SRR4302535.714588/1‑94 (MQ=60)

                                                           GTATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGAT                                    >  SRR4302535.760466/1‑94 (MQ=60)

                                                           GTATTAGCGGTACGGGAATGTGTAATATTCGAAACTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGAT                                    >  SRR4302535.2142395/1‑94 (MQ=60)

                                                             ATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCA                                  <  SRR4302535.2159326/94‑1 (MQ=60)

                                                             ATTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCA                                  >  SRR4302535.214805/1‑94 (MQ=60)

                                                              TTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCAT                                 >  SRR4302535.4379785/1‑94 (MQ=60)

                                                              TTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCAT                                 >  SRR4302535.4118235/1‑94 (MQ=60)

                                                              TTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCAT                                 <  SRR4302535.1298555/94‑1 (MQ=60)

                                                              TTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCAT                                 <  SRR4302535.2121755/94‑1 (MQ=60)

                                                              TTAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCAT                                 <  SRR4302535.535758/94‑1 (MQ=60)

                                                               TAGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATC                                >  SRR4302535.1387367/1‑94 (MQ=60)

                                                                AGCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCT                               >  SRR4302535.627639/1‑94 (MQ=60)

                                                                 GCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAACCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTG                              <  SRR4302535.2209936/94‑1 (MQ=60)

                                                                 GCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTG                              <  SRR4302535.2116065/94‑1 (MQ=60)

                                                                 GCGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTG                              <  SRR4302535.1110708/94‑1 (MQ=60)

                                                                  CGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGG                             <  SRR4302535.1283223/94‑1 (MQ=60)

                                                                  CGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGG                             <  SRR4302535.3463431/94‑1 (MQ=60)

                                                                  CGGTACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGG                             <  SRR4302535.1900265/94‑1 (MQ=60)

                                                                     TACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAAC                          <  SRR4302535.4114932/94‑1 (MQ=60)

                                                                      ACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACG                         <  SRR4302535.556756/94‑1 (MQ=60)

                                                                      ACGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACG                         <  SRR4302535.557042/94‑1 (MQ=60)

                                                                       CGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGA                        >  SRR4302535.891695/1‑94 (MQ=60)

                                                                       CGGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGA                        <  SRR4302535.936244/94‑1 (MQ=60)

                                                                        GGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGAC                       >  SRR4302535.4378284/1‑94 (MQ=60)

                                                                        GGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGAC                       <  SRR4302535.1450750/94‑1 (MQ=60)

                                                                        GGGAATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGAC                       >  SRR4302535.1766231/1‑94 (MQ=60)

                                                                           AATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCCCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCC                    <  SRR4302535.1206210/94‑1 (MQ=60)

                                                                           AATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCC                    >  SRR4302535.840671/1‑94 (MQ=60)

                                                                            ATGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCC                   <  SRR4302535.2828060/94‑1 (MQ=60)

                                                                             TGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCG                  >  SRR4302535.3556557/1‑94 (MQ=60)

                                                                             TGTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCG                  >  SRR4302535.2888027/1‑94 (MQ=60)

                                                                              GTGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGC                 <  SRR4302535.1621705/94‑1 (MQ=60)

                                                                               TGTAATATTCTAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCT                <  SRR4302535.3405165/94‑1 (MQ=60)

                                                                               TGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCT                <  SRR4302535.1777842/94‑1 (MQ=60)

                                                                               TGTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCT                >  SRR4302535.651052/1‑94 (MQ=60)

                                                                                GTAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTC               >  SRR4302535.3499194/1‑94 (MQ=60)

                                                                                 TAATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCA              >  SRR4302535.3446364/1‑94 (MQ=60)

                                                                                   ATATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATT            >  SRR4302535.2350952/1‑94 (MQ=60)

                                                                                    TATTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTG           <  SRR4302535.2637731/94‑1 (MQ=60)

                                                                                    TATTCGAACCTCCAGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTG           <  SRR4302535.3139288/94‑1 (MQ=60)

                                                                                      TTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCC         <  SRR4302535.1174321/94‑1 (MQ=60)

                                                                                      TTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCC         <  SRR4302535.2245626/94‑1 (MQ=60)

                                                                                      TTCGAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCC         <  SRR4302535.1015227/94‑1 (MQ=60)

                                                                                         GAACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATT      <  SRR4302535.1532484/94‑1 (MQ=60)

                                                                                          AACCTCCGGAATCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTA     <  SRR4302535.289874/94‑1 (MQ=60)

                                                                                           ACCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAC    <  SRR4302535.2136659/94‑1 (MQ=60)

                                                                                            CCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACC   <  SRR4302535.1331904/94‑1 (MQ=60)

                                                                                            CCTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACC   >  SRR4302535.2465561/1‑94 (MQ=60)

                                                                                             CTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCA  >  SRR4302535.1277288/1‑94 (MQ=60)

                                                                                             CTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCA  >  SRR4302535.145740/1‑94 (MQ=60)

TGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCA  >  NC_000913/3525237‑3525423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`