Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,556,990 G→A R364H (CGC→CAC)  rtcR → putative 2‑component regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,9900GA99.5% 719.9 / ‑5.5 202R364H (CGC→CAC) rtcRputative 2‑component regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (117/84);  minor base C (1/0);  total (118/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 216 total aligned reads displayed.

TGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGC  >  NC_000913/3556900‑3557079
                                                                                          |                                                                                         
tGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTAcgccagcacc                                                                                         >  2:2102832/1‑93 (MQ=255)
   gcCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAgg                                                                                      >  1:80745/1‑93 (MQ=255)
     cAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGaa                                                                                    >  2:251182/1‑93 (MQ=255)
      aGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAg                                                                                   >  2:3429849/1‑93 (MQ=255)
       gCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGa                                                                                  >  1:1367183/1‑93 (MQ=255)
       gCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGa                                                                                  >  1:1569680/1‑93 (MQ=255)
       gCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGa                                                                                  <  2:1442050/93‑1 (MQ=255)
        cTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGat                                                                                 <  1:2834187/93‑1 (MQ=255)
        cTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGat                                                                                 <  2:2989670/93‑1 (MQ=255)
         ttgTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGCCCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGata                                                                                <  1:489186/91‑1 (MQ=255)
          ggTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGatat                                                                               >  1:4409393/1‑93 (MQ=255)
              gCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaa                                                                           <  1:3352729/93‑1 (MQ=255)
              gCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaa                                                                           <  2:2698091/93‑1 (MQ=255)
                cGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaacc                                                                         >  1:279262/1‑93 (MQ=255)
                  aaGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaaccga                                                                       >  1:13062/1‑93 (MQ=255)
                   aGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaaccgaa                                                                      >  2:3673735/1‑93 (MQ=255)
                   aGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaaccgaa                                                                      <  2:625562/93‑1 (MQ=255)
                    ggCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGCAGATATTgaaccgaac                                                                     >  2:4426031/1‑93 (MQ=255)
                    ggCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaaccgaac                                                                     >  1:3501432/1‑93 (MQ=255)
                    ggCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaaccgaac                                                                     >  2:4026495/1‑93 (MQ=255)
                    ggCAAATTCGGCGAAGATCTGTACGCGCGGACCAATCTCTGGACCTGCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaaccgaac                                                                     <  2:4003613/93‑1 (MQ=255)
                     gCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTgaaccgaacc                                                                    >  1:2729682/1‑93 (MQ=255)
                       aaaTTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCTCCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTg                                                                  <  2:2804651/93‑1 (MQ=255)
                       aaaTTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAGGATATTGAACCGAACCTg                                                                  >  2:397356/1‑93 (MQ=255)
                       aaaTTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTg                                                                  >  1:1998052/1‑93 (MQ=255)
                       aaaTTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTg                                                                  <  1:3731017/93‑1 (MQ=255)
                       aaaTTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTg                                                                  >  1:1215443/1‑93 (MQ=255)
                         aTTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGa                                                                >  2:1216165/1‑93 (MQ=255)
                          tttCGCGAAGATCTGTACGCGCGGATTAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGAt                                                               <  2:4405648/93‑1 (MQ=255)
                          tttCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGAt                                                               <  2:3342205/93‑1 (MQ=255)
                            tCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTa                                                             <  1:2913338/93‑1 (MQ=255)
                            tCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTa                                                             <  2:3004029/93‑1 (MQ=255)
                             cgcgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTAt                                                            <  1:71266/93‑1 (MQ=255)
                              gcgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATg                                                           >  1:4221052/1‑93 (MQ=255)
                              gcgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATg                                                           <  1:4265618/93‑1 (MQ=255)
                              gcgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATg                                                           >  1:1077815/1‑93 (MQ=255)
                              gcgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATg                                                           <  1:1595323/93‑1 (MQ=255)
                               cgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGa                                                          >  1:406015/1‑93 (MQ=255)
                               cgAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGa                                                          <  1:650428/93‑1 (MQ=255)
                                gAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGaa                                                         <  1:141258/93‑1 (MQ=255)
                                gAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGaa                                                         <  2:567917/93‑1 (MQ=255)
                                gAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGaa                                                         <  2:1529775/93‑1 (MQ=255)
                                  aGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGt                                                       <  2:1643082/93‑1 (MQ=255)
                                    aTCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTgg                                                     >  1:3526746/1‑93 (MQ=255)
                                     tCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGa                                                    >  1:2540043/1‑93 (MQ=255)
                                     tCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGa                                                    >  2:2859011/1‑93 (MQ=255)
                                     tCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGa                                                    <  2:1069350/93‑1 (MQ=255)
                                      cTGTACGCGCTGATCAATCTCTGGCCCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAGCCTGGATTATGAAGTGGAg                                                   <  2:1263153/93‑1 (MQ=255)
                                       tGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAgc                                                  >  2:2378980/1‑93 (MQ=255)
                                       tGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAgc                                                  >  1:213064/1‑93 (MQ=255)
                                       tGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAgc                                                  <  2:475963/93‑1 (MQ=255)
                                         tACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAgcgc                                                >  2:4349308/1‑93 (MQ=255)
                                          aCGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGcc                                               <  2:1228051/93‑1 (MQ=255)
                                            gcgcgGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCAc                                             <  1:3701726/93‑1 (MQ=255)
                                                ggATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCct                                         >  1:2968362/1‑93 (MQ=255)
                                                 gATCAATTTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCctc                                        >  2:4608084/1‑93 (MQ=255)
                                                 gATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCATCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCctc                                        <  1:4524482/93‑1 (MQ=255)
                                                    cAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCctcact                                     >  1:1691099/1‑93 (MQ=255)
                                                    cAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCctcact                                     >  1:2253707/1‑93 (MQ=255)
                                                     aaTCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCctcactc                                    >  1:2587015/1‑93 (MQ=255)
                                                       tctcTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCtcactcac                                  <  1:2171987/93‑1 (MQ=255)
                                                        ctctGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAGCCTGGATTATGAAGTGGAGCGCCACGCCTcactcact                                 <  1:1965125/93‑1 (MQ=255)
                                                         tctGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTg                                >  1:2074411/1‑93 (MQ=255)
                                                         tctGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTg                                <  2:3222024/93‑1 (MQ=255)
                                                          ctGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTgg                               >  1:899184/1‑93 (MQ=255)
                                                           tGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGc                              >  2:1569021/1‑93 (MQ=255)
                                                           tGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGc                              >  1:551428/1‑93 (MQ=255)
                                                            ggACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCg                             >  2:3841453/1‑93 (MQ=255)
                                                             gACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGa                            >  2:3663419/1‑93 (MQ=255)
                                                              gccTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGAc                           <  2:2200667/92‑1 (MQ=255)
                                                              aCCTTCACCCTGCCGGGTCTACGCCAGCAACAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGaa                           >  2:1614223/1‑92 (MQ=255)
                                                               ccTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACa                          >  1:2467525/1‑93 (MQ=255)
                                                                cTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAg                         >  2:3555847/1‑93 (MQ=255)
                                                                cTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAg                         >  2:2707919/1‑93 (MQ=255)
                                                                cTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAg                         >  1:3985523/1‑93 (MQ=255)
                                                                   cACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAgcgt                      >  1:974020/1‑93 (MQ=255)
                                                                    aCCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAgcgtg                     >  1:3072305/1‑93 (MQ=255)
                                                                    aCCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTAAGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAgcgtg                     >  2:22910/1‑93 (MQ=255)
                                                                     cccTGCCGGTTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAgcgtgc                    <  1:4093953/93‑1 (MQ=255)
                                                                     cccTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAgcgtgc                    >  1:2404264/1‑93 (MQ=255)
                                                                       cTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAgcgtgcgt                  >  1:3426487/1‑93 (MQ=255)
                                                                       cTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAgcgtgcgt                  >  1:4371248/1‑93 (MQ=255)
                                                                        tGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGtt                 <  1:2154394/93‑1 (MQ=255)
                                                                        tGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGtt                 <  1:4608084/93‑1 (MQ=255)
                                                                        tGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGtt                 <  2:4340904/93‑1 (MQ=255)
                                                                         tccGGGCCTACGCCCACACCGGGAAGCTATTCGACGGAACCTGGTTTGTCAGGTGGCGCGCCACGCCTCACTTACAGGCGCCAGCGAGCGttt                >  2:1742257/2‑93 (MQ=255)
                                                                         gCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCTCTCACTGGCGACAGCGTGCGttt                <  1:4158860/93‑1 (MQ=255)
                                                                         gCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGttt                <  1:2641090/93‑1 (MQ=255)
                                                                           cGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTa              >  2:310335/1‑93 (MQ=255)
                                                                            gggTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTaa             >  1:3924264/1‑93 (MQ=255)
                                                                            gggTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTaa             <  1:2374937/93‑1 (MQ=255)
                                                                              gTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAaca           <  1:4462057/93‑1 (MQ=255)
                                                                              gTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAaca           <  1:3276440/93‑1 (MQ=255)
                                                                               tCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAacac          >  2:200090/1‑93 (MQ=255)
                                                                                cTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACAcc         >  2:3914943/1‑93 (MQ=255)
                                                                                 tACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCg        >  2:4146170/1‑93 (MQ=255)
                                                                                 tACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCg        >  2:486564/1‑93 (MQ=255)
                                                                                  aCGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGa       <  2:2627883/93‑1 (MQ=255)
                                                                                    gccagcaccagGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAg     >  2:1880893/1‑93 (MQ=255)
                                                                                       agcaccagGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAgcgc  <  2:2661894/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGC  >  NC_000913/3556900‑3557079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCG  >  NC_000913/3556897‑3557083
                                                                                             |                                                                                             
ATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCA                                                                                               <  SRR4302535.4343320/94‑1 (MQ=60)
 gtgGCGCCAGCTGGTGGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCAC                                                                                              <  SRR4302535.4159903/91‑1 (MQ=60)
 TTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCAC                                                                                              <  SRR4302535.1332672/94‑1 (MQ=60)
  TTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACC                                                                                             <  SRR4302535.4339711/94‑1 (MQ=60)
   TGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCA                                                                                            <  SRR4302535.2190681/94‑1 (MQ=60)
    GCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAG                                                                                           <  SRR4302535.2639669/94‑1 (MQ=60)
     CGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGG                                                                                          >  SRR4302535.81320/1‑94 (MQ=60)
        CAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAG                                                                                       >  SRR4302535.1298623/1‑94 (MQ=60)
        CAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAG                                                                                       <  SRR4302535.2168627/94‑1 (MQ=60)
         AGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGA                                                                                      >  SRR4302535.1376956/1‑94 (MQ=60)
         AGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGA                                                                                      >  SRR4302535.1581012/1‑94 (MQ=60)
          GCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGAT                                                                                     >  SRR4302535.2798404/1‑94 (MQ=60)
           CTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATA                                                                                    >  SRR4302535.2130290/1‑94 (MQ=60)
           CTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATA                                                                                    <  SRR4302535.1696955/94‑1 (MQ=60)
           CTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATA                                                                                    <  SRR4302535.2854301/94‑1 (MQ=60)
           CTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATA                                                                                    >  SRR4302535.2825927/1‑94 (MQ=60)
            TTGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGCCCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATAT                                                                                   <  SRR4302535.492622/94‑1 (MQ=60)
            TGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATAT                                                                                   >  SRR4302535.4439566/1‑94 (MQ=60)
              GTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTG                                                                                 >  SRR4302535.294477/1‑94 (MQ=60)
                 GCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAAC                                                                              <  SRR4302535.3376079/94‑1 (MQ=60)
                  CCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACC                                                                             >  SRR4302535.281210/1‑94 (MQ=60)
                    GAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGA                                                                           >  SRR4302535.3552469/1‑94 (MQ=60)
                    GAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGA                                                                           >  SRR4302535.13161/1‑94 (MQ=60)
                     AAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAA                                                                          >  SRR4302535.4491208/1‑94 (MQ=60)
                     AAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAA                                                                          >  SRR4302535.4599796/1‑94 (MQ=60)
                      AGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAAC                                                                         >  SRR4302535.405281/1‑94 (MQ=60)
                      AGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAAC                                                                         <  SRR4302535.756244/94‑1 (MQ=60)
                      AGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAAC                                                                         >  SRR4302535.3525773/1‑94 (MQ=60)
                       GGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACC                                                                        >  SRR4302535.2749170/1‑94 (MQ=60)
                       GGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACC                                                                        >  SRR4302535.412122/1‑94 (MQ=60)
                       GGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACC                                                                        >  SRR4302535.3847585/1‑94 (MQ=60)
                         CAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTG                                                                      >  SRR4302535.3736574/1‑94 (MQ=60)
                         CAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTG                                                                      <  SRR4302535.2693658/94‑1 (MQ=60)
                         CAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTG                                                                      >  SRR4302535.1224160/1‑94 (MQ=60)
                         CAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTG                                                                      >  SRR4302535.2012442/1‑94 (MQ=60)
                         CAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCAGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTG                                                                      <  SRR4302535.2535449/94‑1 (MQ=60)
                          AAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGG                                                                     <  SRR4302535.3756853/94‑1 (MQ=60)
                           AATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGA                                                                    >  SRR4302535.4267012/1‑94 (MQ=60)
                               TCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTAT                                                                <  SRR4302535.2933917/94‑1 (MQ=60)
                                CGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATG                                                               >  SRR4302535.1085483/1‑94 (MQ=60)
                                CGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATG                                                               <  SRR4302535.71788/94‑1 (MQ=60)
                                CGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATG                                                               >  SRR4302535.4249936/1‑94 (MQ=60)
                                 GCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGA                                                              >  SRR4302535.408857/1‑94 (MQ=60)
                                 GCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGA                                                              <  SRR4302535.4294829/94‑1 (MQ=60)
                                 GCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGA                                                              <  SRR4302535.1606829/94‑1 (MQ=60)
                                  CGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAA                                                             <  SRR4302535.654966/94‑1 (MQ=60)
                                  CGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAA                                                             >  SRR4302535.955607/1‑94 (MQ=60)
                                  CGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAA                                                             >  SRR4302535.200291/1‑94 (MQ=60)
                                  CGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAA                                                             >  SRR4302535.4651438/1‑94 (MQ=60)
                                   GAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAG                                                            <  SRR4302535.142250/94‑1 (MQ=60)
                                   GAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAG                                                            <  SRR4302535.4372966/94‑1 (MQ=60)
                                     AGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTG                                                          >  SRR4302535.2983304/1‑94 (MQ=60)
                                     AGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTG                                                          >  SRR4302535.808263/1‑94 (MQ=60)
                                      GATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGG                                                         >  SRR4302535.3551234/1‑94 (MQ=60)
                                      GATCTGTACGCGCGGATCAATCTCTGGACCTGCTCCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGG                                                         <  SRR4302535.3470971/94‑1 (MQ=60)
                                       ATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGA                                                        <  SRR4302535.4502351/94‑1 (MQ=60)
                                       ATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGA                                                        >  SRR4302535.2558338/1‑94 (MQ=60)
                                        TCTGTACGCGCGGATCAATCTCTGGCCCTTCGCCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAG                                                       <  SRR4302535.4186625/94‑1 (MQ=60)
                                         CTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGC                                                      >  SRR4302535.214527/1‑94 (MQ=60)
                                         CTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGC                                                      >  SRR4302535.3039913/1‑94 (MQ=60)
                                         CTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGC                                                      <  SRR4302535.3591466/94‑1 (MQ=60)
                                         CTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGC                                                      >  SRR4302535.3970409/1‑94 (MQ=60)
                                          TGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCG                                                     <  SRR4302535.1524306/94‑1 (MQ=60)
                                          TGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCG                                                     >  SRR4302535.2591052/1‑94 (MQ=60)
                                               GCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACG                                                <  SRR4302535.3727363/94‑1 (MQ=60)
                                                  CGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCT                                             >  SRR4302535.4206234/1‑94 (MQ=60)
                                                  CGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCT                                             >  SRR4302535.2989192/1‑94 (MQ=60)
                                                  CGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCT                                             >  SRR4302535.871763/1‑94 (MQ=60)
                                                   GGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCACCACGCCTC                                            >  SRR4302535.1699802/1‑94 (MQ=60)
                                                    GATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCATCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCA                                           <  SRR4302535.4555479/94‑1 (MQ=60)
                                                     ATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCAC                                          >  SRR4302535.3373790/1‑94 (MQ=60)
                                                     ATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCAC                                          <  SRR4302535.4496002/94‑1 (MQ=60)
                                                      TCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACT                                         >  SRR4302535.2269759/1‑94 (MQ=60)
                                                      TCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACT                                         >  SRR4302535.1703336/1‑94 (MQ=60)
                                                       CAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTC                                        >  SRR4302535.4430012/1‑94 (MQ=60)
                                                       CAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTC                                        <  SRR4302535.1335627/94‑1 (MQ=60)
                                                       CAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTC                                        >  SRR4302535.2605593/1‑94 (MQ=60)
                                                        AATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCA                                       >  SRR4302535.1718006/1‑94 (MQ=60)
                                                        AATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCA                                       >  SRR4302535.1406094/1‑94 (MQ=60)
                                                         ATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCAC                                      >  SRR4302535.3381140/1‑94 (MQ=60)
                                                          TCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACT                                     <  SRR4302535.2187531/94‑1 (MQ=60)
                                                           CTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAGCCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTG                                    <  SRR4302535.1979290/94‑1 (MQ=60)
                                                           CTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTG                                    >  SRR4302535.2089353/1‑94 (MQ=60)
                                                            TCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGG                                   >  SRR4302535.905553/1‑94 (MQ=60)
                                                            TCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGG                                   <  SRR4302535.2081096/94‑1 (MQ=60)
                                                            TCTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGG                                   <  SRR4302535.2890719/94‑1 (MQ=60)
                                                             CTGGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGC                                  >  SRR4302535.555288/1‑94 (MQ=60)
                                                               GGACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGA                                >  SRR4302535.2479710/1‑94 (MQ=60)
                                                                 ACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACA                              >  SRR4302535.2485313/1‑94 (MQ=60)
                                                                 ACCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACA                              >  SRR4302535.158383/1‑94 (MQ=60)
                                                                  CCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAG                             >  SRR4302535.4012761/1‑94 (MQ=60)
                                                                  CCTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAG                             >  SRR4302535.259586/1‑94 (MQ=60)
                                                                   CTTCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGA                            >  SRR4302535.55410/1‑94 (MQ=60)
                                                                     TCACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGT                          >  SRR4302535.980933/1‑94 (MQ=60)
                                                                      CACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGTCACGCCTCACTCACTGGCGACAGCGTG                         <  SRR4302535.4138842/94‑1 (MQ=60)
                                                                      CACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTG                         >  SRR4302535.3093755/1‑94 (MQ=60)
                                                                      CACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTG                         >  SRR4302535.21815/1‑94 (MQ=60)
                                                                       ACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGC                        >  SRR4302535.2182961/1‑94 (MQ=60)
                                                                       ACCCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGC                        >  SRR4302535.2421563/1‑94 (MQ=60)
                                                                        CCCTGCCGGTTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCG                       <  SRR4302535.4121969/94‑1 (MQ=60)
                                                                         CCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGT                      >  SRR4302535.1129067/1‑94 (MQ=60)
                                                                         CCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGT                      >  SRR4302535.3450311/1‑94 (MQ=60)
                                                                         CCTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGT                      >  SRR4302535.4401159/1‑94 (MQ=60)
                                                                          CTGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTT                     >  SRR4302535.717193/1‑94 (MQ=60)
                                                                           TGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTT                    <  SRR4302535.4639398/94‑1 (MQ=60)
                                                                           TGCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTT                    <  SRR4302535.2169877/94‑1 (MQ=60)
                                                                            GCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCTCTCACTGGCGACAGCGTGCGTTTT                   <  SRR4302535.4187325/94‑1 (MQ=60)
                                                                            GCCGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTT                   <  SRR4302535.2660009/94‑1 (MQ=60)
                                                                              CGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAA                 >  SRR4302535.2417938/1‑94 (MQ=60)
                                                                              CGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAA                 >  SRR4302535.2391589/1‑94 (MQ=60)
                                                                              CGGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAA                 >  SRR4302535.3951081/1‑94 (MQ=60)
                                                                               GGGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAAC                <  SRR4302535.2391999/94‑1 (MQ=60)
                                                                                GGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACA               >  SRR4302535.623100/1‑94 (MQ=60)
                                                                                GGTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACA               >  SRR4302535.3530328/1‑94 (MQ=60)
                                                                                 GTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACAC              <  SRR4302535.3299200/94‑1 (MQ=60)
                                                                                 GTCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACAC              <  SRR4302535.4492660/94‑1 (MQ=60)
                                                                                  TCTACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACC             <  SRR4302535.3097460/94‑1 (MQ=60)
                                                                                     ACGCCAGCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAA          >  SRR4302535.997243/1‑94 (MQ=60)
                                                                                       GCCAGCACCACGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCCCCACGCCTCCCTCACTGGCGACAGCGTGCGTTTTAACACCGAAGC        <  SRR4302535.1486975/94‑1 (MQ=60)
                                                                                           GCACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGG    <  SRR4302535.4635660/94‑1 (MQ=60)
                                                                                            CACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGC   <  SRR4302535.573153/94‑1 (MQ=60)
                                                                                            CACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGC   >  SRR4302535.3877512/1‑94 (MQ=60)
                                                                                            CACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGC   >  SRR4302535.3068317/1‑94 (MQ=60)
                                                                                             ACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCG  >  SRR4302535.142200/1‑94 (MQ=60)
                                                                                             ACCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCG  >  SRR4302535.1256448/1‑94 (MQ=60)
                                                                                             |                                                                                             
ATTTGCGCCAGCTGGTTGCCGAAGGCAAATTTCGCGAAGATCTGTACGCGCGGATCAATCTCTGGACCTTCACCCTGCCGGGTCTACGCCAGCGCCAGGAAGATATTGAACCGAACCTGGATTATGAAGTGGAGCGCCACGCCTCACTCACTGGCGACAGCGTGCGTTTTAACACCGAAGCGCGGCG  >  NC_000913/3556897‑3557083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑