Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,572,116 G→A G132G (GGC→GGT)  asd ← aspartate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,572,1160GA99.7% 1022.2 / ‑5.7 287G132G (GGC→GGT) asdaspartate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (145/141);  total (146/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01

Only 100 of 300 total aligned reads displayed.

CTGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCGCCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCAT  >  NC_000913/3572026‑3572205
                                                                                          |                                                                                         
 tGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTAccacca                                                                                        >  1:4369270/1‑93 (MQ=255)
  ggTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACcaa                                                                                       <  1:1145178/93‑1 (MQ=255)
    tAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCCAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACcaaca                                                                                     <  2:2745821/93‑1 (MQ=255)
       gTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACaaaa                                                                                  <  1:1315099/93‑1 (MQ=255)
        ttGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAg                                                                                 <  2:1492792/93‑1 (MQ=255)
         tGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGt                                                                                <  1:3147287/93‑1 (MQ=255)
          gCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTc                                                                               <  1:4406365/93‑1 (MQ=255)
            aaCGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCt                                                                             >  1:2478847/1‑93 (MQ=255)
               ggACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGAt                                                                          <  1:1845070/93‑1 (MQ=255)
                gACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATg                                                                         >  2:3717498/1‑93 (MQ=255)
                    cccAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCat                                                                     >  2:3187845/1‑93 (MQ=255)
                      cAATCAACAAGATCATTGGCGAATAAACCGCCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCatta                                                                   <  2:412268/93‑1 (MQ=255)
                      cAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCatta                                                                   <  1:540087/93‑1 (MQ=255)
                      cAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCatta                                                                   <  1:3956758/93‑1 (MQ=255)
                      cAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCatta                                                                   <  2:3404623/93‑1 (MQ=255)
                        aTCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACCTCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCattatt                                                                 <  1:2684339/93‑1 (MQ=255)
                        aTCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCattatt                                                                 >  1:288045/1‑93 (MQ=255)
                        aTCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCattatt                                                                 <  2:2907664/93‑1 (MQ=255)
                         tCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTAttt                                                                >  1:2359175/1‑93 (MQ=255)
                         acaacaaGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGTTACAGTTACCACCAACAAAAGTCCTGATGCCATTAttt                                                                <  2:2081782/92‑1 (MQ=255)
                           aacaaGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTaa                                                              <  2:1219081/93‑1 (MQ=255)
                           aacaaGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTaa                                                              >  2:3020682/1‑93 (MQ=255)
                           aacaaGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTaa                                                              <  1:2963350/93‑1 (MQ=255)
                            acaaGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAAt                                                             >  1:3753759/1‑93 (MQ=255)
                              aaGATCATTGGCGAATAAACCCCCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATcc                                                           <  1:3384413/93‑1 (MQ=255)
                               aGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCg                                                          >  2:1853419/1‑93 (MQ=255)
                               aGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCg                                                          >  1:2626053/1‑93 (MQ=255)
                                gATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGt                                                         >  1:4009084/1‑93 (MQ=255)
                                 aTCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTc                                                        <  2:2614188/93‑1 (MQ=255)
                                 aTCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTc                                                        >  2:409021/1‑93 (MQ=255)
                                 aTCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTc                                                        >  1:1824887/1‑93 (MQ=255)
                                 aTCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTc                                                        <  1:3882421/93‑1 (MQ=255)
                                   cATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCgg                                                      <  1:4132963/93‑1 (MQ=255)
                                   cATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCgg                                                      >  1:2376103/1‑93 (MQ=255)
                                    aTTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGt                                                     <  1:3277584/93‑1 (MQ=255)
                                    aTTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGt                                                     <  2:1924181/93‑1 (MQ=255)
                                     ttGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTa                                                    >  1:1701866/1‑93 (MQ=255)
                                      tGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTaa                                                   <  2:2242855/93‑1 (MQ=255)
                                      tGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTaa                                                   <  1:2755871/93‑1 (MQ=255)
                                       ggCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAAt                                                  <  2:4018237/93‑1 (MQ=255)
                                       ggCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAAt                                                  <  2:767725/93‑1 (MQ=255)
                                         cGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGa                                                >  2:2724997/1‑93 (MQ=255)
                                          gAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGAc                                               <  2:1036863/93‑1 (MQ=255)
                                           aaTAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACg                                              >  1:578568/1‑93 (MQ=255)
                                              aaaCCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTcc                                           <  1:3647161/93‑1 (MQ=255)
                                               aaCCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCt                                          <  1:2221695/93‑1 (MQ=255)
                                               aaCCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCt                                          >  1:2560262/1‑93 (MQ=255)
                                                accaccCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTg                                         >  2:4521229/1‑93 (MQ=255)
                                                  cccccAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAt                                       <  1:1458963/91‑1 (MQ=255)
                                                  caccCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAt                                       >  2:610193/1‑93 (MQ=255)
                                                  caccCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAt                                       <  2:3958968/93‑1 (MQ=255)
                                                  caccCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAt                                       <  2:3467461/93‑1 (MQ=255)
                                                  caccCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAt                                       >  1:3089411/1‑93 (MQ=255)
                                                   accCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAtt                                      >  1:4283048/1‑93 (MQ=255)
                                                   accCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAtt                                      <  1:1347700/93‑1 (MQ=255)
                                                   accCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAtt                                      >  1:3044618/1‑93 (MQ=255)
                                                       aaCGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACg                                  >  2:2212836/1‑93 (MQ=255)
                                                       aaCGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACg                                  >  2:3835910/1‑93 (MQ=255)
                                                        aCGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACgg                                 <  1:661839/93‑1 (MQ=255)
                                                        aCGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACgg                                 <  1:4286915/93‑1 (MQ=255)
                                                        aCGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACgg                                 <  1:19415/93‑1 (MQ=255)
                                                        aCGACATCAACATCAGGCTCACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACgg                                 >  2:4563937/1‑93 (MQ=255)
                                                          gACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACgggg                               >  2:1773329/1‑93 (MQ=255)
                                                           gcatcaacatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTAAATCCGTCGGTAATGACGTCCTGATTGACGGGGt                              <  2:3374350/92‑1 (MQ=255)
                                                             atcaacatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCa                            >  2:1313550/1‑93 (MQ=255)
                                                             atcaacatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCa                            >  1:4619800/1‑93 (MQ=255)
                                                              tcaacatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCaa                           >  2:490130/1‑93 (MQ=255)
                                                              tcaacatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCaa                           >  2:2372147/1‑93 (MQ=255)
                                                              tcaacatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCaa                           <  1:2022651/93‑1 (MQ=255)
                                                               caacatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAg                          >  2:1455091/1‑93 (MQ=255)
                                                                aacatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGa                         <  2:1742081/93‑1 (MQ=255)
                                                                 acatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGaa                        <  2:4304091/93‑1 (MQ=255)
                                                                 acatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGaa                        <  2:940168/93‑1 (MQ=255)
                                                                 acatcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGaa                        <  2:1529372/93‑1 (MQ=255)
                                                                  catcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAat                       >  1:1087980/1‑93 (MQ=255)
                                                                   atcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAatg                      >  2:1156190/1‑93 (MQ=255)
                                                                    tcaGGCTTACGGTACAGTTACCACCGACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAatca                     <  1:4529860/93‑3 (MQ=255)
                                                                    tcaGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAatga                     >  2:3221882/1‑93 (MQ=255)
                                                                     caGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAatgat                    <  1:4162077/93‑1 (MQ=255)
                                                                        gCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAatgatgat                 >  1:2151291/1‑93 (MQ=255)
                                                                               gTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCa          >  1:787328/1‑93 (MQ=255)
                                                                               gTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCa          >  1:350351/1‑93 (MQ=255)
                                                                                 aCAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATc        <  1:231315/93‑1 (MQ=255)
                                                                                  cAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCt       <  2:3491807/93‑1 (MQ=255)
                                                                                  cAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCt       >  2:2152008/1‑93 (MQ=255)
                                                                                  cAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCt       <  1:1596250/93‑1 (MQ=255)
                                                                                  cAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCt       >  2:728489/1‑93 (MQ=255)
                                                                                  cAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCt       <  2:1659248/93‑1 (MQ=255)
                                                                                   aGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCtt      <  1:3636347/93‑1 (MQ=255)
                                                                                   aGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCtt      <  2:299550/93‑1 (MQ=255)
                                                                                    gTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCttt     >  1:3223560/1‑93 (MQ=255)
                                                                                     ttACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTc    <  2:3619276/93‑1 (MQ=255)
                                                                                      tACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCa   <  2:12650/93‑1 (MQ=255)
                                                                                      tACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCa   <  2:4628237/93‑1 (MQ=255)
                                                                                      tACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCa   <  1:4264960/93‑1 (MQ=255)
                                                                                      tACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCa   <  1:3577594/93‑1 (MQ=255)
                                                                                      tACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCa   <  1:3822218/93‑1 (MQ=255)
                                                                                      tACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCa   <  2:4070836/93‑1 (MQ=255)
                                                                                       accaccAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCAt  <  1:1269477/93‑1 (MQ=255)
                                                                                       accaccAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCAt  <  1:1744115/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCGCCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCAT  >  NC_000913/3572026‑3572205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCCTGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCGCCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCATGCGC  >  NC_000913/3572023‑3572209
                                                                                             |                                                                                             
GGCCTGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCA                                                                                               <  SRR4302535.2013076/94‑1 (MQ=60)
GGCCTGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCA                                                                                               <  SRR4302535.1721783/94‑1 (MQ=60)
GGCCTGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCA                                                                                               >  SRR4302535.1193834/1‑94 (MQ=60)
  CCTGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACC                                                                                             >  SRR4302535.1136032/1‑94 (MQ=60)
   CTGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCA                                                                                            >  SRR4302535.4399172/1‑94 (MQ=60)
    TGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAA                                                                                           >  SRR4302535.3861647/1‑94 (MQ=60)
    TGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAA                                                                                           >  SRR4302535.4098694/1‑94 (MQ=60)
     GGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAAC                                                                                          >  SRR4302535.1383219/1‑94 (MQ=60)
     GGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAAC                                                                                          <  SRR4302535.1153339/94‑1 (MQ=60)
      GTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACA                                                                                         >  SRR4302535.2230160/1‑94 (MQ=60)
      GTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACA                                                                                         >  SRR4302535.4029203/1‑94 (MQ=60)
       TAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAA                                                                                        <  SRR4302535.1542735/94‑1 (MQ=60)
          GTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAG                                                                                     >  SRR4302535.2483376/1‑94 (MQ=60)
          GTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAG                                                                                     <  SRR4302535.1324495/94‑1 (MQ=60)
          GTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAG                                                                                     >  SRR4302535.453453/1‑94 (MQ=60)
          GTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAG                                                                                     <  SRR4302535.2948091/94‑1 (MQ=60)
          GTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAG                                                                                     >  SRR4302535.634691/1‑94 (MQ=60)
           TTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGT                                                                                    <  SRR4302535.2724505/94‑1 (MQ=60)
            TGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTC                                                                                   >  SRR4302535.1295689/1‑94 (MQ=60)
            TGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTC                                                                                   <  SRR4302535.3169168/94‑1 (MQ=60)
            TGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTC                                                                                   >  SRR4302535.1544443/1‑94 (MQ=60)
            TGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTC                                                                                   >  SRR4302535.4696882/1‑94 (MQ=60)
             GCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCC                                                                                  <  SRR4302535.4436521/94‑1 (MQ=60)
              CAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACTTCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCT                                                                                 <  SRR4302535.2534565/94‑1 (MQ=60)
              CAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCT                                                                                 >  SRR4302535.2496727/1‑94 (MQ=60)
              CAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCT                                                                                 <  SRR4302535.237909/94‑1 (MQ=60)
               AACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTG                                                                                <  SRR4302535.578028/94‑1 (MQ=60)
               AACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTG                                                                                >  SRR4302535.1715344/1‑94 (MQ=60)
               AACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTG                                                                                <  SRR4302535.1151570/94‑1 (MQ=60)
               AACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTG                                                                                <  SRR4302535.3261641/94‑1 (MQ=60)
                 CGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGAT                                                                              <  SRR4302535.3667986/94‑1 (MQ=60)
                 CGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGAT                                                                              <  SRR4302535.3583279/94‑1 (MQ=60)
                  GGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATG                                                                             <  SRR4302535.1858370/94‑1 (MQ=60)
                  GGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATG                                                                             >  SRR4302535.3080417/1‑94 (MQ=60)
                  GGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATG                                                                             >  SRR4302535.1709576/1‑94 (MQ=60)
                   GACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGC                                                                            <  SRR4302535.3672040/94‑1 (MQ=60)
                     CACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCA                                                                          >  SRR4302535.2870121/1‑94 (MQ=60)
                     CACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCA                                                                          >  SRR4302535.4361187/1‑94 (MQ=60)
                     CACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCA                                                                          <  SRR4302535.3426868/94‑1 (MQ=60)
                      ACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCAT                                                                         >  SRR4302535.2664547/1‑94 (MQ=60)
                      ACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCAT                                                                         >  SRR4302535.2664546/1‑94 (MQ=60)
                       ACCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATT                                                                        <  SRR4302535.2141022/94‑1 (MQ=60)
                        CCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTA                                                                       <  SRR4302535.2637995/94‑1 (MQ=60)
                        CCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTA                                                                       <  SRR4302535.1352730/94‑1 (MQ=60)
                         CAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTAT                                                                      <  SRR4302535.1981897/94‑1 (MQ=60)
                         CAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTAT                                                                      <  SRR4302535.3983773/94‑1 (MQ=60)
                         CAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTAT                                                                      <  SRR4302535.543856/94‑1 (MQ=60)
                          AATCAACAAGATCATTGGCGAATAAGCCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATT                                                                     <  SRR4302535.3118113/94‑1 (MQ=60)
                          AATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATT                                                                     <  SRR4302535.3905794/94‑1 (MQ=60)
                          AATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATT                                                                     >  SRR4302535.241465/1‑94 (MQ=60)
                          AATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATT                                                                     >  SRR4302535.290063/1‑94 (MQ=60)
                           ATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACCTCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTT                                                                    <  SRR4302535.2703534/94‑1 (MQ=60)
                           ATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTT                                                                    <  SRR4302535.984294/94‑1 (MQ=60)
                           ATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTT                                                                    <  SRR4302535.3425087/94‑1 (MQ=60)
                           ATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTT                                                                    >  SRR4302535.2376104/1‑94 (MQ=60)
                           ATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTT                                                                    <  SRR4302535.1942227/94‑1 (MQ=60)
                             CAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAA                                                                  >  SRR4302535.1777173/1‑94 (MQ=60)
                             CAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAA                                                                  >  SRR4302535.4177554/1‑94 (MQ=60)
                              AACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAAT                                                                 >  SRR4302535.3779732/1‑94 (MQ=60)
                              AACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAAT                                                                 <  SRR4302535.2984150/94‑1 (MQ=60)
                                CAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCC                                                               >  SRR4302535.3307774/1‑94 (MQ=60)
                                CAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCC                                                               <  SRR4302535.2615705/94‑1 (MQ=60)
                                 AAGATCATTGGCGAATAAACCCCCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCG                                                              <  SRR4302535.3407961/94‑1 (MQ=60)
                                 AAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCG                                                              >  SRR4302535.2644872/1‑94 (MQ=60)
                                 AAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCG                                                              <  SRR4302535.238809/94‑1 (MQ=60)
                                 AAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCG                                                              >  SRR4302535.397729/1‑94 (MQ=60)
                                 AAGAACATTGGCGAATAAACCACCCAACGACATCAACAGCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCG                                                              <  SRR4302535.2246020/94‑1 (MQ=60)
                                  AGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGT                                                             >  SRR4302535.4036509/1‑94 (MQ=60)
                                  AGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGT                                                             >  SRR4302535.588666/1‑94 (MQ=60)
                                  AGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGC                                                             >  SRR4302535.1299963/1‑94 (MQ=60)
                                   GATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTC                                                            >  SRR4302535.1838062/1‑94 (MQ=60)
                                   GATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTC                                                            <  SRR4302535.1327662/94‑1 (MQ=60)
                                    ATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCG                                                           <  SRR4302535.3909059/94‑1 (MQ=60)
                                    ATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCG                                                           >  SRR4302535.4306501/1‑94 (MQ=60)
                                     TCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGG                                                          >  SRR4302535.2393172/1‑94 (MQ=60)
                                      CATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGT                                                         <  SRR4302535.4161239/94‑1 (MQ=60)
                                      CATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGA                                                         >  SRR4302535.4499598/1‑94 (MQ=60)
                                       ATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTA                                                        <  SRR4302535.3300353/94‑1 (MQ=60)
                                       ATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTA                                                        >  SRR4302535.4218247/1‑94 (MQ=60)
                                       ATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTA                                                        >  SRR4302535.1714169/1‑94 (MQ=60)
                                         TGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAAT                                                      <  SRR4302535.2775514/94‑1 (MQ=60)
                                          GGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATG                                                     <  SRR4302535.2792520/94‑1 (MQ=60)
                                          GGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATG                                                     <  SRR4302535.703351/94‑1 (MQ=60)
                                           GCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGA                                                    <  SRR4302535.3006478/94‑1 (MQ=60)
                                            CGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGAC                                                   <  SRR4302535.1843265/94‑1 (MQ=60)
                                            CGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGAC                                                   >  SRR4302535.4308360/1‑94 (MQ=60)
                                            CGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGAC                                                   >  SRR4302535.4315144/1‑94 (MQ=60)
                                            CGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGAC                                                   <  SRR4302535.4719843/94‑1 (MQ=60)
                                            CGAATAAACCACCCAACGACATCAACATCAGGCTTACGGGACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTGATGAC                                                   >  SRR4302535.4409903/1‑94 (MQ=60)
                                             GAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACG                                                  >  SRR4302535.1257110/1‑94 (MQ=60)
                                             GAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACG                                                  <  SRR4302535.3250472/94‑1 (MQ=60)
                                             GAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACG                                                  >  SRR4302535.582635/1‑94 (MQ=60)
                                              AATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGT                                                 >  SRR4302535.2248270/1‑94 (MQ=60)
                                              AATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGT                                                 >  SRR4302535.4356888/1‑94 (MQ=60)
                                              AATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGA                                                 >  SRR4302535.3777688/1‑94 (MQ=60)
                                                TAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCC                                               <  SRR4302535.1703269/94‑1 (MQ=60)
                                                 AAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCT                                              <  SRR4302535.573966/94‑1 (MQ=60)
                                                 AAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCT                                              <  SRR4302535.3672413/94‑1 (MQ=60)
                                                 AAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCT                                              >  SRR4302535.3068690/1‑94 (MQ=60)
                                                 AAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCT                                              >  SRR4302535.1843788/1‑94 (MQ=60)
                                                 AAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCT                                              <  SRR4302535.2499757/94‑1 (MQ=60)
                                                 AAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCT                                              >  SRR4302535.2578694/1‑94 (MQ=60)
                                                  AACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTG                                             <  SRR4302535.2237512/94‑1 (MQ=60)
                                                    CCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAT                                           >  SRR4302535.3110928/1‑94 (MQ=60)
                                                    CCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAT                                           >  SRR4302535.2989671/1‑94 (MQ=60)
                                                    CCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGAT                                           >  SRR4302535.4416223/1‑94 (MQ=60)
                                                     CCCCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATT                                          <  SRR4302535.1469418/94‑1 (MQ=60)
                                                     CACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATT                                          >  SRR4302535.3065944/1‑94 (MQ=60)
                                                     CACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATT                                          >  SRR4302535.4312350/1‑94 (MQ=60)
                                                      ACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTG                                         <  SRR4302535.1357320/94‑1 (MQ=60)
                                                      ACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTG                                         <  SRR4302535.2035411/94‑1 (MQ=60)
                                                      ACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTG                                         <  SRR4302535.4277090/94‑1 (MQ=60)
                                                        CCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGAC                                       >  SRR4302535.3783494/1‑94 (MQ=60)
                                                        CCAACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGAC                                       >  SRR4302535.1253045/1‑94 (MQ=60)
                                                          AACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGG                                     >  SRR4302535.2217484/1‑94 (MQ=60)
                                                           ACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGG                                    <  SRR4302535.3162625/94‑1 (MQ=60)
                                                           ACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGG                                    <  SRR4302535.4316240/94‑1 (MQ=60)
                                                           ACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGG                                    <  SRR4302535.19559/94‑1 (MQ=60)
                                                           ACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGG                                    <  SRR4302535.3619438/94‑1 (MQ=60)
                                                           ACGACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGG                                    <  SRR4302535.666458/94‑1 (MQ=60)
                                                             GACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGT                                  >  SRR4302535.4236702/1‑94 (MQ=60)
                                                             GACATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGT                                  <  SRR4302535.2347683/94‑1 (MQ=60)
                                                               CATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCA                                >  SRR4302535.4651190/1‑94 (MQ=60)
                                                               CATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCA                                >  SRR4302535.1725118/1‑94 (MQ=60)
                                                                ATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAA                               >  SRR4302535.1826129/1‑94 (MQ=60)
                                                                ATCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAA                               >  SRR4302535.1952217/1‑94 (MQ=60)
                                                                 TCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAG                              >  SRR4302535.3342888/1‑94 (MQ=60)
                                                                 TCAACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAG                              <  SRR4302535.2037230/94‑1 (MQ=60)
                                                                   AACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAA                            >  SRR4302535.3829899/1‑94 (MQ=60)
                                                                   AACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAA                            >  SRR4302535.4313492/1‑94 (MQ=60)
                                                                    ACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAAT                           >  SRR4302535.1095720/1‑94 (MQ=60)
                                                                    ACATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAAT                           <  SRR4302535.2944174/94‑1 (MQ=60)
                                                                     CATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATG                          >  SRR4302535.3575844/1‑94 (MQ=60)
                                                                     CATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATG                          >  SRR4302535.2125592/1‑94 (MQ=60)
                                                                     CATCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATG                          >  SRR4302535.4562508/1‑94 (MQ=60)
                                                                       TCAGGCTTACGGTACAGTTACCACCGACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATCAT                        <  SRR4302535.4560873/94‑1 (MQ=60)
                                                                       TCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGAT                        <  SRR4302535.2711588/94‑1 (MQ=60)
                                                                       TCAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGAT                        <  SRR4302535.3238504/94‑1 (MQ=60)
                                                                        CAGGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATG                       <  SRR4302535.4190566/94‑1 (MQ=60)
                                                                          GGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGAT                     <  SRR4302535.463464/94‑1 (MQ=60)
                                                                          GGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGAT                     >  SRR4302535.3956371/1‑94 (MQ=60)
                                                                          GGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGAT                     >  SRR4302535.2166756/1‑94 (MQ=60)
                                                                          GGCTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGAT                     <  SRR4302535.3984261/94‑1 (MQ=60)
                                                                            CTTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGG                   <  SRR4302535.2372557/94‑1 (MQ=60)
                                                                             TTACGGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGC                  >  SRR4302535.3767434/1‑94 (MQ=60)
                                                                                 GGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCA              >  SRR4302535.792850/1‑94 (MQ=60)
                                                                                 GGTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCA              >  SRR4302535.352766/1‑94 (MQ=60)
                                                                                  GTACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCAT             <  SRR4302535.4144131/94‑1 (MQ=60)
                                                                                    ACAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCT           <  SRR4302535.232912/94‑1 (MQ=60)
                                                                                     CAGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTT          <  SRR4302535.1607765/94‑1 (MQ=60)
                                                                                      AGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTT         >  SRR4302535.3245904/1‑94 (MQ=60)
                                                                                      AGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTT         >  SRR4302535.3210117/1‑94 (MQ=60)
                                                                                      AGTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTT         <  SRR4302535.3661545/94‑1 (MQ=60)
                                                                                       GTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTC        <  SRR4302535.695495/94‑1 (MQ=60)
                                                                                       GTTACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTC        >  SRR4302535.36071/1‑94 (MQ=60)
                                                                                         TACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCAT      <  SRR4302535.1396713/94‑1 (MQ=60)
                                                                                         TACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCAT      <  SRR4302535.3848585/94‑1 (MQ=60)
                                                                                         TACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCAT      <  SRR4302535.4294167/94‑1 (MQ=60)
                                                                                         TACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCAT      <  SRR4302535.3602396/94‑1 (MQ=60)
                                                                                          ACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCATG     <  SRR4302535.1756734/94‑1 (MQ=60)
                                                                                          ACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCATG     <  SRR4302535.1278549/94‑1 (MQ=60)
                                                                                          ACCACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCATG     >  SRR4302535.3178935/1‑94 (MQ=60)
                                                                                            CACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCATGCG   >  SRR4302535.4078438/1‑94 (MQ=60)
                                                                                             ACCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCATGCGC  >  SRR4302535.3922945/1‑94 (MQ=60)
                                                                                             |                                                                                             
GGCCTGGTAGGTTGCAACGGACACCCAATCAACAAGATCATTGGCGAATAAACCACCCAACGACATCAACATCAGGCTTACGGTACAGTTACCGCCAACAAAAGTCCTGATGCCATTATTTAATCCGTCGGTAATGACGTCCTGATTGACGGGGTCAAGAATGATGATGGCGTCATCTTTCATGCGC  >  NC_000913/3572023‑3572209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑