Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,572,523 (T)7→6 intergenic (‑12/‑181) asd ← / → yhgN aspartate‑semialdehyde dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,572,5170T.99.6% 1051.0 / ‑3.0 232intergenic (‑6/‑187)asd/yhgNaspartate‑semialdehyde dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base . (109/122);  total (109/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

GGCGTCGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAA  >  NC_000913/3572425‑3572605
                                                                                            |                                                                                        
gtggTCAAAGTCGCGCTCTCCAACCATGTGTCGCATGAGAAGGGAGCCTCCCATACCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCGt                                                                                          <  2:14948/90‑1 (MQ=255)
ggCGTCGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGt                                                                                          <  2:1630270/93‑1 (MQ=255)
  ggTCGAAGTCGCGCTCTTCAACCATGCGTGGCAGGAGAGCGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttt                                                                                        <  1:1257893/92‑1 (MQ=255)
   gTCGAGGTCTCGCTCTCCAACTGTGCGTTGCATGAGACCGGACCCGTCCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGtttt                                                                                       <  2:3031546/93‑1 (MQ=255)
   gTCGAGGTCGCGCTCTTCACCCATGCGTTGCATGAGAAGGGAGCCGATCATCCCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCGtttt                                                                                       <  2:3741543/93‑1 (MQ=255)
   gTCGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGtttt                                                                                       >  1:2754683/1‑93 (MQ=255)
      gAAGTCGCGCTCTTCAACCCTGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttttttc                                                                                    >  2:4576060/1‑92 (MQ=255)
      gAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttttttc                                                                                    >  2:2689154/1‑92 (MQ=255)
      gAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttttttc                                                                                    >  2:3104450/1‑92 (MQ=255)
       aggTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGACCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttttttcc                                                                                   <  1:1109587/91‑3 (MQ=255)
       aaGTCGCGCTCTACAACCATGGTTTGCATGAGAACGGACCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGttttttcc                                                                                   <  2:4130936/93‑3 (MQ=255)
             ccccctTCAACCATGCTTTGCATGAGAACGGAGCCAACCATACCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCaaa                                                                            <  1:3686495/89‑1 (MQ=255)
              gcTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAg                                                                           >  2:2556235/1‑93 (MQ=255)
               cttttCAACCATGCGTTGCAGGAGAACGGAGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGa                                                                          <  1:2149543/90‑1 (MQ=255)
               ctctTCACCCATGCTTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGa                                                                          <  1:1507576/93‑1 (MQ=255)
               ctctTCACCCATGCGTTGCATGAGAACGGACCCGACCATACCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGa                                                                          <  1:536490/93‑1 (MQ=255)
               ctctTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGa                                                                          >  2:3208502/1‑93 (MQ=255)
                tctTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAt                                                                         >  1:2343929/1‑93 (MQ=255)
                tctTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAt                                                                         >  1:2498293/1‑93 (MQ=255)
                tctTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAt                                                                         >  2:2212657/1‑93 (MQ=255)
                tctTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAt                                                                         >  2:1942683/1‑93 (MQ=255)
                tctTCAACCATGCGTTGCATGAGAACGGAACCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAt                                                                         <  1:3396147/93‑1 (MQ=255)
                 ctTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAtg                                                                        >  2:239941/1‑93 (MQ=255)
                  ttCAACCAAGCGTTTCAGGAAAACGGACCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAtgt                                                                       <  1:1489596/93‑1 (MQ=255)
                   taaaCCATGCATTGCATGAGAACGGAGCAGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAtgtg                                                                      <  1:4679293/91‑1 (MQ=255)
                    cAACCATGCGTTGCATGAGAACGGAGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAtgtgt                                                                     <  2:1303320/93‑1 (MQ=255)
                    cAACCATGCGTTGCATGAGAACGGAGCCGACAATACCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAtgtgt                                                                     <  2:1342167/93‑1 (MQ=255)
                     aaCCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAtgtgtg                                                                    >  1:2389653/1‑93 (MQ=255)
                     aaCCATGCGTTGCATGAGAACGGAGCCGACAATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAtgtgtg                                                                    >  2:3464213/1‑93 (MQ=255)
                     aaCCATGCGTTGCATGAGAACGGACCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAtgtgtg                                                                    <  1:2842444/93‑1 (MQ=255)
                       ccATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCt                                                                  >  1:1533968/1‑93 (MQ=255)
                          tGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGta                                                               >  2:3081627/1‑93 (MQ=255)
                           gCGTTGCATGAGACCGGAGCCGCCCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGtat                                                              <  1:1577254/93‑1 (MQ=255)
                           gCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGtat                                                              >  2:382383/1‑93 (MQ=255)
                             gTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATaa                                                            >  1:3479394/1‑93 (MQ=255)
                             gTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGCGTGCTGTATaa                                                            >  2:4290528/1‑93 (MQ=255)
                              ttGCATGAGAACGGCTGCGACCATAGCGCGCCCGCCGATCAATCCAACATTTTTCATACACG‑ATTTTTCCTCCAGAGATGTGTTCTTtatata                                                           >  2:3132402/1‑91 (MQ=255)
                              tggCATGAGAACGGACCCGCCCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATaaa                                                           <  2:1123885/91‑1 (MQ=255)
                               tGCATGAGAACGGAGCCGCCCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAAt                                                          <  2:176925/93‑1 (MQ=255)
                                 cATGAAACCGGAGCCGACCATCCCCCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAAtgt                                                        <  2:770870/93‑1 (MQ=255)
                                    gagaACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGcc                                                     >  1:2939755/1‑93 (MQ=255)
                                     agaAGGGAGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCg                                                    <  1:3895561/93‑1 (MQ=255)
                                      gaACGCAGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCgg                                                   <  2:3665041/93‑1 (MQ=255)
                                       aaCGAACCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGt                                                  <  1:2603085/93‑1 (MQ=255)
                                         cGGAGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGtct                                                <  1:961131/93‑1 (MQ=255)
                                           gAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTctcc                                              >  2:1146850/1‑93 (MQ=255)
                                             gCCGACCATCCCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTctcctc                                            <  1:926496/93‑1 (MQ=255)
                                             gCCAACAATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTctcctc                                            <  1:1185739/93‑1 (MQ=255)
                                                 aCCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTgg                                        >  2:3417598/1‑93 (MQ=255)
                                                  ccATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGc                                       >  1:2315788/1‑93 (MQ=255)
                                                   cATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGca                                      >  1:3640843/1‑93 (MQ=255)
                                                   cATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGca                                      >  1:3339175/1‑93 (MQ=255)
                                                   cATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGca                                      >  1:1816070/1‑93 (MQ=255)
                                                    aTACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGcac                                     >  2:3260556/1‑93 (MQ=255)
                                                     tACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGcaca                                    >  2:316106/1‑93 (MQ=255)
                                                     acccGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGcaca                                    <  1:3734314/91‑1 (MQ=255)
                                                      aCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACAt                                   >  2:2082099/1‑93 (MQ=255)
                                                           gcCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTc                              >  1:1260496/1‑93 (MQ=255)
                                                           gcCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTc                              >  2:4109712/1‑93 (MQ=255)
                                                           cccAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTc                              <  2:3612706/92‑1 (MQ=255)
                                                              aGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCAcc                           >  2:4001212/1‑93 (MQ=255)
                                                                ccGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCAt                         <  1:605228/93‑1 (MQ=255)
                                                                 cGATAAAACCAACATTTTTCATAGGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATa                        <  1:866264/93‑1 (MQ=255)
                                                                 cGATAAAACCAACATTTTTCATAAGCG‑GTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATa                        >  2:848586/1‑93 (MQ=255)
                                                                  gATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATAc                       >  2:4650640/1‑93 (MQ=255)
                                                                   aTAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACa                      <  1:3155661/93‑1 (MQ=255)
                                                                   aTAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACa                      <  2:2930384/93‑1 (MQ=255)
                                                                    tAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACaa                     >  2:533351/1‑93 (MQ=255)
                                                                     aaaaCCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACaaa                    >  1:2067424/1‑93 (MQ=255)
                                                                      aaaCCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACaaaa                   >  1:2808641/1‑93 (MQ=255)
                                                                      aaaCCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACaaaa                   <  1:1733918/93‑1 (MQ=255)
                                                                      aaaCCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACaaaa                   <  1:1069371/93‑1 (MQ=255)
                                                                      aaaCCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACaaaa                   >  2:605874/1‑93 (MQ=255)
                                                                       aaCCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACaaaaa                  >  2:2779961/1‑93 (MQ=255)
                                                                         ccAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagc                <  2:2969568/93‑1 (MQ=255)
                                                                         ccAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagc                >  2:38798/1‑93 (MQ=255)
                                                                         ccAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagc                >  1:266876/1‑93 (MQ=255)
                                                                          cAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagca               >  1:1420720/1‑93 (MQ=255)
                                                                          cAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagca               <  2:3370846/93‑1 (MQ=255)
                                                                          cAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagca               >  2:1668659/1‑93 (MQ=255)
                                                                          cAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagca               >  1:4224925/1‑93 (MQ=255)
                                                                          cAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagca               <  1:2044398/93‑1 (MQ=255)
                                                                          cAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagca               >  1:4433000/1‑93 (MQ=255)
                                                                           aaCATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagcag              >  1:1877262/1‑93 (MQ=255)
                                                                            aCATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAagcagc             <  1:4553833/93‑1 (MQ=255)
                                                                             cATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGcc            <  1:728489/93‑1 (MQ=255)
                                                                                ttttCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCaaa         <  2:1906085/93‑1 (MQ=255)
                                                                                ttttCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCaaa         <  2:2634375/93‑1 (MQ=255)
                                                                                  ttCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGt       <  1:4375028/93‑1 (MQ=255)
                                                                                  ttCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGt       <  1:1631241/93‑1 (MQ=255)
                                                                                  ttCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGt       <  1:2265402/93‑1 (MQ=255)
                                                                                  ttCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGt       <  2:4271488/93‑1 (MQ=255)
                                                                                  ttCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGt       <  1:873629/93‑1 (MQ=255)
                                                                                   tCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTc      >  2:2935106/1‑93 (MQ=255)
                                                                                   tCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTc      <  2:4395669/93‑1 (MQ=255)
                                                                                   tCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTc      <  1:4275250/93‑1 (MQ=255)
                                                                                      tAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCa   >  1:4072054/1‑93 (MQ=255)
                                                                                       aaGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCaa  >  2:4543635/1‑93 (MQ=255)
                                                                                       aaGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCaa  <  2:2431921/93‑1 (MQ=255)
                                                                                       aaGCG‑TTTTTTCCTGCAAAGATGGGTGCTGTAGAAATGTGCCGGTCTCCTCTTGACACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCaa  <  2:900431/93‑1 (MQ=255)
                                                                                            |                                                                                        
GGCGTCGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAA  >  NC_000913/3572425‑3572605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAAT  >  NC_000913/3572430‑3572616
                                                                                             |                                                                                             
CGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTC                                                                                               >  SRR4302535.340650/1‑94 (MQ=60)
CGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTC                                                                                               >  SRR4302535.927987/1‑94 (MQ=60)
 gaagtcctgttcttaAACCATGCTTTGCATAAGAACGAACCGGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTCC                                                                                              <  SRR4302535.2234810/79‑1 (MQ=57)
 GAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTCC                                                                                              >  SRR4302535.4215646/1‑94 (MQ=60)
  AGGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGACCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCT                                                                                            <  SRR4302535.1117512/94‑1 (MQ=60)
  AAGGCGCGCTCTTCACCCATGCGTGGCAGGAGAACGGAGCCGCCCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCT                                                                                            <  SRR4302535.2351897/94‑1 (MQ=60)
   AGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTG                                                                                           >  SRR4302535.2931748/1‑94 (MQ=60)
   AGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTG                                                                                           <  SRR4302535.2947883/94‑1 (MQ=60)
   AGCCGCCCTCTTCAACCATGCGTTGCAGGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTG                                                                                           <  SRR4302535.3970215/94‑1 (MQ=60)
     gcgccttctcCAACCATGCGTGGCGTGAAAACGGACCCGCCCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCA                                                                                         <  SRR4302535.4107921/84‑1 (MQ=55)
     TCGCTCTCTTCAACCATGCGTGTCATGAGAACGGACCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCA                                                                                         <  SRR4302535.2072829/94‑1 (MQ=60)
        ccccCTTCAACCATGCTTTGCATGAGAACGGAGCCAACCATACCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAG                                                                                      <  SRR4302535.3711999/90‑1 (MQ=60)
        CGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAG                                                                                      >  SRR4302535.1688206/1‑94 (MQ=60)
        CGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAG                                                                                      <  SRR4302535.2403930/94‑1 (MQ=60)
        CGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAG                                                                                      >  SRR4302535.2043290/1‑94 (MQ=60)
        CGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAG                                                                                      >  SRR4302535.1592512/1‑94 (MQ=60)
         GCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCTGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGA                                                                                     >  SRR4302535.855962/1‑94 (MQ=60)
          CTTTTCAACCATGCGTTGCAGGAGAACGGAGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAT                                                                                    <  SRR4302535.2165003/94‑1 (MQ=60)
          CTCTTCACCCATGCTTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAT                                                                                    <  SRR4302535.1518415/94‑1 (MQ=60)
          CTCTTCACCCATGCGTTGCATGAGAACGGACCCGACCATACCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAT                                                                                    <  SRR4302535.540238/94‑1 (MQ=60)
          CTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAT                                                                                    >  SRR4302535.2360700/1‑94 (MQ=60)
          CTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAT                                                                                    >  SRR4302535.4317840/1‑94 (MQ=60)
          CTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGAT                                                                                    >  SRR4302535.2516316/1‑94 (MQ=60)
           TCTTCAACCATGCGTTGCATGAGAACGGAACCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATG                                                                                   <  SRR4302535.3419774/94‑1 (MQ=60)
            CTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGT                                                                                  >  SRR4302535.2847218/1‑94 (MQ=60)
            CTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGT                                                                                  >  SRR4302535.475151/1‑94 (MQ=60)
            CTCCACCCATGCGTTACATGAGAACGTACCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGT                                                                                  <  SRR4302535.1036020/94‑1 (MQ=60)
             TTCACCCATGCGTTGCAGGAGAACGGACCCGACAATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTG                                                                                 <  SRR4302535.2459716/94‑1 (MQ=60)
             TTCAACCAAGCGTTTCAGGAAAACGGACCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTG                                                                                 <  SRR4302535.1500286/94‑1 (MQ=60)
              TAAACCATGCATTGCATGAGAACGGAGCAGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGT                                                                                <  SRR4302535.4710874/94‑1 (MQ=60)
               CAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTG                                                                               >  SRR4302535.2406832/1‑94 (MQ=60)
                AACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGC                                                                              >  SRR4302535.2734276/1‑94 (MQ=60)
                AACCATGCGTTGCATGAGAACGGACCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGC                                                                              <  SRR4302535.2862601/94‑1 (MQ=60)
                 ACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCT                                                                             >  SRR4302535.1545019/1‑94 (MQ=60)
                    ctcctttcCATGAAAACGGAGCCGACCATCCCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTA                                                                          <  SRR4302535.3169314/86‑1 (MQ=60)
                    cggggttgcgggagaagggccCCGACCATCCCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTA                                                                          <  SRR4302535.2791820/73‑1 (MQ=60)
                     TGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTAT                                                                         >  SRR4302535.1269355/1‑94 (MQ=60)
                      GCGTTGCATGAGACCGGAGCCGCCCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATA                                                                        <  SRR4302535.1588636/94‑1 (MQ=60)
                       CTTTGCATGAGAACGGACCCGACCATCCCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAA                                                                       <  SRR4302535.1584145/94‑1 (MQ=60)
                       CGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAA                                                                       >  SRR4302535.2250893/1‑94 (MQ=60)
                       CGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAA                                                                       >  SRR4302535.3503567/1‑94 (MQ=60)
                       CGTGGCTTGAGAGCGGAGCCGACATTCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAA                                                                       <  SRR4302535.1539323/94‑1 (MQ=60)
                        GTGGCATGAGAACGGAGCGGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAA                                                                      <  SRR4302535.877779/94‑1 (MQ=60)
                           GCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGT                                                                   >  SRR4302535.1975653/1‑94 (MQ=60)
                           GCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGT                                                                   >  SRR4302535.144994/1‑94 (MQ=60)
                           GCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGT                                                                   >  SRR4302535.3709048/1‑94 (MQ=60)
                            CTTGAAAACGGAGCAGACAATGCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTG                                                                  <  SRR4302535.1095602/94‑1 (MQ=60)
                            CATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTG                                                                  <  SRR4302535.2670881/94‑1 (MQ=60)
                            CATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTG                                                                  <  SRR4302535.2306702/94‑1 (MQ=60)
                            CATGACACCGGAGCCGACAATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTG                                                                  <  SRR4302535.2641291/94‑1 (MQ=60)
                             ATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGC                                                                 >  SRR4302535.2573570/1‑94 (MQ=60)
                              TGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCC                                                                >  SRR4302535.2960430/1‑94 (MQ=60)
                              TGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCC                                                                <  SRR4302535.1481763/94‑1 (MQ=60)
                               GAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCG                                                               >  SRR4302535.1751745/1‑94 (MQ=60)
                                AGAAGGGAGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGG                                                              <  SRR4302535.3922258/94‑1 (MQ=60)
                                AGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGG                                                              >  SRR4302535.2312722/1‑94 (MQ=60)
                                  AACGAACCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTC                                                            <  SRR4302535.2621771/94‑1 (MQ=60)
                                   acggatcggtccatcCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCT                                                           <  SRR4302535.2073074/79‑1 (MQ=60)
                                   ACGGACCCGCCCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCT                                                           <  SRR4302535.950933/94‑1 (MQ=60)
                                    CGGAGCCGACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTC                                                          <  SRR4302535.967959/94‑1 (MQ=60)
                                       acccacCCATACCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTC                                                       <  SRR4302535.2451035/88‑1 (MQ=60)
                                        acccacaatcCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCT                                                      <  SRR4302535.1794848/84‑1 (MQ=60)
                                        GCCGACCATCCCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCT                                                      <  SRR4302535.933059/94‑1 (MQ=60)
                                        GCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCT                                                      <  SRR4302535.652697/94‑1 (MQ=60)
                                        GCCAACAATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCT                                                      <  SRR4302535.1194208/94‑1 (MQ=60)
                                         CCGACAAGACCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTT                                                     <  SRR4302535.4300717/94‑1 (MQ=60)
                                          CGACCATCCCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTG                                                    <  SRR4302535.4619528/94‑1 (MQ=60)
                                           CACCATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGG                                                   <  SRR4302535.4060771/94‑1 (MQ=60)
                                            ACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGC                                                  >  SRR4302535.2332357/1‑94 (MQ=60)
                                             CCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCA                                                 >  SRR4302535.3362434/1‑94 (MQ=60)
                                             CCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCA                                                 >  SRR4302535.3666064/1‑94 (MQ=60)
                                             CCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCA                                                 >  SRR4302535.1829199/1‑94 (MQ=60)
                                              CATCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCAC                                                <  SRR4302535.3369974/94‑1 (MQ=60)
                                                tccccCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACAT                                              <  SRR4302535.1132964/89‑1 (MQ=60)
                                                acccgccCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACAT                                              <  SRR4302535.3760160/87‑1 (MQ=60)
                                                TGCCGCCCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACAT                                              <  SRR4302535.307810/94‑1 (MQ=60)
                                                TCCCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACAT                                              <  SRR4302535.1362488/94‑1 (MQ=60)
                                                  CCGC‑CCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTT                                           <  SRR4302535.936550/94‑1 (MQ=60)
                                                   CGCGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTT                                           <  SRR4302535.1214690/94‑1 (MQ=60)
                                                    cccCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTT                                          <  SRR4302535.3426200/91‑1 (MQ=60)
                                                     CGCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTC                                         >  SRR4302535.1269516/1‑94 (MQ=60)
                                                      CCCAGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCA                                        <  SRR4302535.3735923/94‑1 (MQ=60)
                                                         AGCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCA                                     >  SRR4302535.117208/1‑94 (MQ=60)
                                                          GCCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCAT                                    <  SRR4302535.1521235/94‑1 (MQ=60)
                                                           CCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATA                                   <  SRR4302535.609460/94‑1 (MQ=60)
                                                           CCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATA                                   >  SRR4302535.2779100/1‑94 (MQ=60)
                                                           CCGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATA                                   <  SRR4302535.1729631/94‑1 (MQ=60)
                                                            CGATAAAACCAACATTTTTCATAGGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATAC                                  <  SRR4302535.872390/94‑1 (MQ=60)
                                                            CGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCTTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATAC                                  >  SRR4302535.567793/1‑94 (MQ=60)
                                                            CGATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATAC                                  <  SRR4302535.2489790/94‑1 (MQ=60)
                                                              ATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAA                                <  SRR4302535.4404929/94‑1 (MQ=60)
                                                              ATAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAA                                <  SRR4302535.3177578/94‑1 (MQ=60)
                                                               TAAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAA                               >  SRR4302535.2082326/1‑94 (MQ=60)
                                                                AAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAA                              >  SRR4302535.2828609/1‑94 (MQ=60)
                                                                AAAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAA                              >  SRR4302535.3004085/1‑94 (MQ=60)
                                                                 AAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAA                             <  SRR4302535.1076972/94‑1 (MQ=60)
                                                                 AAACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAA                             <  SRR4302535.1746449/94‑1 (MQ=60)
                                                                  AACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAG                            >  SRR4302535.3277631/1‑94 (MQ=60)
                                                                  AACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAG                            >  SRR4302535.131736/1‑94 (MQ=60)
                                                                  AACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAG                            <  SRR4302535.808742/94‑1 (MQ=60)
                                                                   ACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGC                           >  SRR4302535.4439098/1‑94 (MQ=60)
                                                                   ACCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGC                           >  SRR4302535.268729/1‑94 (MQ=60)
                                                                    CCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAACAAGCA                          >  SRR4302535.1508934/1‑94 (MQ=60)
                                                                    CCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCA                          >  SRR4302535.4253833/1‑94 (MQ=60)
                                                                    CCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCA                          >  SRR4302535.4463376/1‑94 (MQ=60)
                                                                    CCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCA                          >  SRR4302535.3887170/1‑94 (MQ=60)
                                                                    CCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCA                          >  SRR4302535.1430864/1‑94 (MQ=60)
                                                                    CCAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCA                          >  SRR4302535.1150787/1‑94 (MQ=60)
                                                                     CAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACACAAAGCAG                         >  SRR4302535.3718308/1‑94 (MQ=60)
                                                                     CAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAG                         >  SRR4302535.1890785/1‑94 (MQ=60)
                                                                     CAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAG                         >  SRR4302535.2759340/1‑94 (MQ=60)
                                                                     CAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAG                         <  SRR4302535.931892/94‑1 (MQ=60)
                                                                     CAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAG                         >  SRR4302535.4450816/1‑94 (MQ=60)
                                                                     CAACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAG                         <  SRR4302535.2059125/94‑1 (MQ=60)
                                                                      AACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGC                        >  SRR4302535.2366391/1‑94 (MQ=60)
                                                                       ACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCC                       >  SRR4302535.4644377/1‑94 (MQ=60)
                                                                       ACATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCC                       <  SRR4302535.4584944/94‑1 (MQ=60)
                                                                        CATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCA                      <  SRR4302535.733601/94‑1 (MQ=60)
                                                                         ATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAA                     >  SRR4302535.2095723/1‑94 (MQ=60)
                                                                         ATTTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAA                     >  SRR4302535.3170125/1‑94 (MQ=60)
                                                                           TTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAG                   <  SRR4302535.3682650/94‑1 (MQ=60)
                                                                           TTTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAG                   <  SRR4302535.1832797/94‑1 (MQ=60)
                                                                            TTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGT                  <  SRR4302535.3551317/94‑1 (MQ=60)
                                                                            TTTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGT                  <  SRR4302535.3503/94‑1 (MQ=60)
                                                                             TTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTC                 <  SRR4302535.4404962/94‑1 (MQ=60)
                                                                             TTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTC                 <  SRR4302535.1000909/94‑1 (MQ=60)
                                                                             TTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTC                 <  SRR4302535.1570928/94‑1 (MQ=60)
                                                                             TTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTC                 <  SRR4302535.879820/94‑1 (MQ=60)
                                                                             TTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTC                 <  SRR4302535.48234/94‑1 (MQ=60)
                                                                             TTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTC                 <  SRR4302535.2281543/94‑1 (MQ=60)
                                                                             TTCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTC                 <  SRR4302535.1643023/94‑1 (MQ=60)
                                                                              TCATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCG                <  SRR4302535.4304515/94‑1 (MQ=60)
                                                                               CATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGC               >  SRR4302535.4075292/1‑94 (MQ=60)
                                                                               CAGAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGC               >  SRR4302535.4561874/1‑94 (MQ=60)
                                                                                ATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCA              >  SRR4302535.4099920/1‑94 (MQ=60)
                                                                                ATAAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCA              <  SRR4302535.1799232/94‑1 (MQ=60)
                                                                                  AAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAG            <  SRR4302535.638754/94‑1 (MQ=60)
                                                                                  AAGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAG            >  SRR4302535.1916814/1‑94 (MQ=60)
                                                                                   AGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGT           <  SRR4302535.3426129/94‑1 (MQ=60)
                                                                                   AGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGT           <  SRR4302535.304168/94‑1 (MQ=60)
                                                                                   AGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGT           <  SRR4302535.310785/94‑1 (MQ=60)
                                                                                   AGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGT           >  SRR4302535.1002683/1‑94 (MQ=60)
                                                                                   AGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGT           <  SRR4302535.26788/94‑1 (MQ=60)
                                                                                   AGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGT           >  SRR4302535.2381273/1‑94 (MQ=60)
                                                                                   AGCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGT           <  SRR4302535.4364720/94‑1 (MQ=60)
                                                                                    GCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTG          >  SRR4302535.1797509/1‑94 (MQ=60)
                                                                                    GCG‑TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTG          >  SRR4302535.1157349/1‑94 (MQ=60)
                                                                                      cgTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGA         <  SRR4302535.2902468/92‑1 (MQ=60)
                                                                                       GTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAA        <  SRR4302535.2882991/94‑1 (MQ=60)
                                                                                       GTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAA        >  SRR4302535.3658249/1‑94 (MQ=60)
                                                                                       GTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAA        >  SRR4302535.2318133/1‑94 (MQ=60)
                                                                                        TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAA       <  SRR4302535.4073637/94‑1 (MQ=60)
                                                                                        TTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAA       >  SRR4302535.4174086/1‑94 (MQ=60)
                                                                                          TTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATT     <  SRR4302535.619442/94‑1 (MQ=60)
                                                                                           TTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTA    >  SRR4302535.353180/1‑94 (MQ=60)
                                                                                            TTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAA   <  SRR4302535.2348752/94‑1 (MQ=60)
                                                                                             TCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAAT  <  SRR4302535.2517420/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGAAGTCGCGCTCTTCAACCATGCGTTGCATGAGAACGGAGCCGACCATACCGCGCCAGCCGATAAAACCAACATTTTTCATAAGCGTTTTTTTCCTGCAAAGATGTGTGCTGTATAAATGTGCCGGTCTCCTCTTGGCACATCTTTCACCATACAAAAAGCAGCCAAAGTCGCAAGTGAAATTAAT  >  NC_000913/3572430‑3572616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑