Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,705,464 T→C intergenic (‑130/+781) dppA ← / ← proK dipeptide transport protein/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,705,4640TC99.6% 723.6 / ‑5.1 228intergenic (‑130/+781)dppA/proKdipeptide transport protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (114/113);  total (115/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 231 total aligned reads displayed.

GCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTT  >  NC_000913/3705375‑3705553
                                                                                         |                                                                                         
gCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTa                                                                                        >  2:4638/1‑93 (MQ=255)
  aGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAAc                                                                                      <  2:568934/93‑1 (MQ=255)
  aGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAAc                                                                                      >  1:3989209/1‑93 (MQ=255)
  aGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAAc                                                                                      <  2:1299686/93‑1 (MQ=255)
   gTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACa                                                                                     >  1:4271239/1‑93 (MQ=255)
   gTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACa                                                                                     >  1:3367411/1‑93 (MQ=255)
    tGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAg                                                                                    >  2:2161766/1‑93 (MQ=255)
     gggTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGt                                                                                   <  1:2867031/93‑1 (MQ=255)
     gggTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGt                                                                                   <  2:3125905/93‑1 (MQ=255)
      ggTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTg                                                                                  <  2:3762640/93‑1 (MQ=255)
       gTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGc                                                                                 <  1:2734856/93‑1 (MQ=255)
       gTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGAGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGc                                                                                 >  2:2529188/1‑93 (MQ=255)
        ttGTCGCTGCCTGGCCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCg                                                                                <  2:222973/93‑1 (MQ=255)
        ttGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCg                                                                                <  1:4358026/93‑1 (MQ=255)
          gTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGa                                                                              >  1:3078775/1‑93 (MQ=255)
             gCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGAtcc                                                                           >  1:3182848/1‑93 (MQ=255)
             gCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGAtcc                                                                           >  2:188038/1‑93 (MQ=255)
             gCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGAtcc                                                                           <  2:2606658/93‑1 (MQ=255)
               tGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGAtcctc                                                                         >  2:3693758/1‑93 (MQ=255)
                gCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGAtcctcc                                                                        >  2:2443064/1‑93 (MQ=255)
                 ccTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCa                                                                       <  2:1304568/93‑1 (MQ=255)
                 ccTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCa                                                                       <  1:3815610/93‑1 (MQ=255)
                 ccTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCa                                                                       <  1:4649506/93‑1 (MQ=255)
                 ccTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCa                                                                       <  2:3594822/93‑1 (MQ=255)
                 ccTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCa                                                                       <  1:2555193/93‑1 (MQ=255)
                 ccTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAAAATCAGCGTAACAGTGCGGATCCTCCa                                                                       >  1:3390997/1‑93 (MQ=255)
                   tGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATa                                                                     <  2:4163631/93‑1 (MQ=255)
                   tGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATa                                                                     >  1:4065755/1‑93 (MQ=255)
                   tGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATa                                                                     >  1:156659/1‑93 (MQ=255)
                   tGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATa                                                                     >  1:2338944/1‑93 (MQ=255)
                   tGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATa                                                                     >  1:3951573/1‑93 (MQ=255)
                     aCCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATaaa                                                                   <  1:2821880/93‑1 (MQ=255)
                     aCCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATaaa                                                                   <  1:1817683/93‑1 (MQ=255)
                     aCCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATaaa                                                                   >  2:3026898/1‑93 (MQ=255)
                     aCCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATaaa                                                                   >  2:2131473/1‑93 (MQ=255)
                      ccTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATaaaa                                                                  >  1:2984948/1‑93 (MQ=255)
                      ccTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATaaaa                                                                  >  1:2772788/1‑93 (MQ=255)
                      ccTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATaaaa                                                                  <  2:1298146/93‑1 (MQ=255)
                      ccTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATaaaa                                                                  <  2:1598779/93‑1 (MQ=255)
                       cTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAAt                                                                 >  1:357864/1‑93 (MQ=255)
                        ttttttgttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAAtc                                                                >  1:973312/1‑92 (MQ=255)
                        ttttttgttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATg                                                                >  1:1035586/1‑93 (MQ=255)
                          ttttgttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGcc                                                              <  1:1449995/93‑1 (MQ=255)
                          ttttgttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGcc                                                              >  1:81348/1‑93 (MQ=255)
                           tttgttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGccc                                                             <  2:4557184/93‑1 (MQ=255)
                              gttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTc                                                          >  1:2787044/1‑93 (MQ=255)
                              gttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTc                                                          <  1:1698144/93‑1 (MQ=255)
                               ttttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCg                                                         >  2:233555/1‑93 (MQ=255)
                                tttttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGt                                                        <  2:4376692/93‑1 (MQ=255)
                                   ttgCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGAc                                                     <  2:491117/93‑1 (MQ=255)
                                     gCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCt                                                   <  2:1478649/93‑1 (MQ=255)
                                        cGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACa                                                >  1:2599298/1‑93 (MQ=255)
                                         ggTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACaa                                               >  2:4396566/1‑93 (MQ=255)
                                         ggTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACaa                                               >  2:3304485/1‑93 (MQ=255)
                                         ggTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACaa                                               >  1:3057560/1‑93 (MQ=255)
                                          gTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAAt                                              >  1:912107/1‑93 (MQ=255)
                                          gTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAAt                                              >  2:2790340/1‑93 (MQ=255)
                                          gTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAAt                                              >  2:2041902/1‑93 (MQ=255)
                                           tCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATc                                             <  2:2844116/93‑1 (MQ=255)
                                             gggTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTg                                           <  1:4519149/93‑1 (MQ=255)
                                             gggTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTg                                           >  2:1972837/1‑93 (MQ=255)
                                                 cAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCaa                                       <  1:3549000/93‑1 (MQ=255)
                                                  aaCGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAAc                                      >  2:1758644/1‑93 (MQ=255)
                                                  aaCGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAAc                                      <  2:2739753/93‑1 (MQ=255)
                                                  aaCGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAAc                                      >  2:2687167/1‑93 (MQ=255)
                                                   aCGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACa                                     >  2:3456495/1‑93 (MQ=255)
                                                   aCGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACa                                     >  1:1162009/1‑93 (MQ=255)
                                                     gTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGa                                   <  2:4387517/93‑1 (MQ=255)
                                                      ttATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGaa                                  >  1:1924163/1‑93 (MQ=255)
                                                        aTGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAAtg                                <  1:2038828/93‑1 (MQ=255)
                                                         tGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAAtgt                               <  2:556384/93‑1 (MQ=255)
                                                         tGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAAtgt                               >  1:644001/1‑93 (MQ=255)
                                                            ggTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGCATGTGaa                            >  1:2466069/1‑93 (MQ=255)
                                                            ggTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGaa                            <  2:1534514/93‑1 (MQ=255)
                                                            ggTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGaa                            <  2:170702/93‑1 (MQ=255)
                                                              tGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGaaaa                          <  2:3439883/93‑1 (MQ=255)
                                                                   aTGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCa                     >  2:1746263/1‑93 (MQ=255)
                                                                     gCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAAt                   >  1:3256048/1‑93 (MQ=255)
                                                                       cGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAACTGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATAc                 <  1:2490437/93‑1 (MQ=255)
                                                                       cGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATAc                 <  1:4486976/93‑1 (MQ=255)
                                                                        gTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACa                >  1:3678435/1‑93 (MQ=255)
                                                                        gTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACa                <  2:1830545/93‑1 (MQ=255)
                                                                         tACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAg               >  1:707612/1‑93 (MQ=255)
                                                                           cTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGcc             >  1:3288653/1‑93 (MQ=255)
                                                                           cTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGcc             >  1:2642542/1‑93 (MQ=255)
                                                                            tAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCa            >  1:4190296/1‑93 (MQ=255)
                                                                            tAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCa            <  1:4586161/93‑1 (MQ=255)
                                                                              aTTAACATCAGCGTAACAGTGCGTATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAc          <  1:1537136/93‑1 (MQ=255)
                                                                              aTTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAc          <  2:427536/93‑1 (MQ=255)
                                                                              aTTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAc          <  1:4040181/93‑1 (MQ=255)
                                                                               ttAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAcc         <  2:609740/93‑1 (MQ=255)
                                                                               ttAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAcc         <  2:3168755/93‑1 (MQ=255)
                                                                               ttAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAcc         <  1:1061067/93‑1 (MQ=255)
                                                                                 aaCATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCgg       >  2:1455103/1‑93 (MQ=255)
                                                                                  aCATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAATCggg      >  1:736781/1‑93 (MQ=255)
                                                                                   cATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACCGAATGTGAAAACGTCAATACAGCCAACCGTGa     >  2:3569155/1‑93 (MQ=255)
                                                                                   cATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGa     <  1:4504183/93‑1 (MQ=255)
                                                                                    aTCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGAt    <  2:2887315/93‑1 (MQ=255)
                                                                                    aTCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGAt    >  1:1283884/1‑93 (MQ=255)
                                                                                     tCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGAtt   >  2:4078235/1‑93 (MQ=255)
                                                                                         |                                                                                         
GCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTT  >  NC_000913/3705375‑3705553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTAC  >  NC_000913/3705371‑3705555
                                                                                             |                                                                                           
CTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGC                                                                                             >  SRR4302535.3951859/1‑94 (MQ=60)
CTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGC                                                                                             >  SRR4302535.837393/1‑94 (MQ=60)
CTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGC                                                                                             >  SRR4302535.583236/1‑94 (MQ=60)
 TTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCG                                                                                            <  SRR4302535.1845573/94‑1 (MQ=60)
 TTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCG                                                                                            >  SRR4302535.2564829/1‑94 (MQ=60)
     CAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAAC                                                                                        >  SRR4302535.2502277/1‑94 (MQ=60)
     CAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAAC                                                                                        <  SRR4302535.1739895/94‑1 (MQ=60)
     CAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAAC                                                                                        >  SRR4302535.4016493/1‑94 (MQ=60)
      AGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACA                                                                                       >  SRR4302535.3390842/1‑94 (MQ=60)
      AGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACA                                                                                       >  SRR4302535.752932/1‑94 (MQ=60)
      AGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACA                                                                                       >  SRR4302535.4300479/1‑94 (MQ=60)
         GGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTG                                                                                    <  SRR4302535.2887340/94‑1 (MQ=60)
           GTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCG                                                                                  <  SRR4302535.2754369/94‑1 (MQ=60)
           GTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCG                                                                                  >  SRR4302535.4141667/1‑94 (MQ=60)
            TTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGG                                                                                 <  SRR4302535.4387861/94‑1 (MQ=60)
            TTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGG                                                                                 <  SRR4302535.3403482/94‑1 (MQ=60)
             TGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGA                                                                                >  SRR4302535.3100247/1‑94 (MQ=60)
                CGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCC                                                                             >  SRR4302535.3204840/1‑94 (MQ=60)
                 GCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCT                                                                            >  SRR4302535.2496615/1‑94 (MQ=60)
                   TGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCC                                                                          >  SRR4302535.1466182/1‑94 (MQ=60)
                    GCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAAAATCAGCGTAACAGTGCGGATCCTCCA                                                                         >  SRR4302535.3414588/1‑94 (MQ=60)
                     CCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCAT                                                                        <  SRR4302535.2573595/94‑1 (MQ=60)
                     CCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCAT                                                                        >  SRR4302535.2641531/1‑94 (MQ=60)
                     CCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCAT                                                                        <  SRR4302535.4680996/94‑1 (MQ=60)
                     CCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCAT                                                                        >  SRR4302535.1424819/1‑94 (MQ=60)
                     CCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCAT                                                                        <  SRR4302535.3841935/94‑1 (MQ=60)
                      CTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATC                                                                       >  SRR4302535.4534211/1‑94 (MQ=60)
                      CTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATA                                                                       >  SRR4302535.1958314/1‑94 (MQ=60)
                      CTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATA                                                                       >  SRR4302535.2355680/1‑94 (MQ=60)
                      CTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATA                                                                       >  SRR4302535.4093575/1‑94 (MQ=60)
                      CTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATA                                                                       >  SRR4302535.157766/1‑94 (MQ=60)
                      CTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATA                                                                       >  SRR4302535.3978553/1‑94 (MQ=60)
                       TGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAA                                                                      >  SRR4302535.46288/1‑94 (MQ=60)
                       TGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAA                                                                      >  SRR4302535.2587397/1‑94 (MQ=60)
                         ACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAA                                                                    >  SRR4302535.3005885/1‑94 (MQ=60)
                         ACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAA                                                                    <  SRR4302535.2841917/94‑1 (MQ=60)
                         ACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAA                                                                    >  SRR4302535.2792531/1‑94 (MQ=60)
                         ACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAA                                                                    <  SRR4302535.1830819/94‑1 (MQ=60)
                          CCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGTTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAAT                                                                   <  SRR4302535.1864182/94‑1 (MQ=60)
                          CCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAAT                                                                   >  SRR4302535.360339/1‑94 (MQ=60)
                           CTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATG                                                                  >  SRR4302535.1042940/1‑94 (MQ=60)
                           CTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATC                                                                  >  SRR4302535.980220/1‑94 (MQ=60)
                             TTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCC                                                                >  SRR4302535.4036148/1‑94 (MQ=60)
                             TTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCC                                                                >  SRR4302535.81927/1‑94 (MQ=60)
                              TTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCC                                                               >  SRR4302535.3984330/1‑94 (MQ=60)
                              TTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCC                                                               <  SRR4302535.1460365/94‑1 (MQ=60)
                                 TGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTC                                                            <  SRR4302535.961688/94‑1 (MQ=60)
                                 TGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTC                                                            >  SRR4302535.2806875/1‑94 (MQ=60)
                                  GTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCG                                                           <  SRR4302535.1710417/94‑1 (MQ=60)
                                  GTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCG                                                           >  SRR4302535.305420/1‑94 (MQ=60)
                                     TTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGA                                                        >  SRR4302535.594362/1‑94 (MQ=60)
                                       TTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACC                                                      <  SRR4302535.2882845/94‑1 (MQ=60)
                                          CCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTAC                                                   <  SRR4302535.2747653/94‑1 (MQ=60)
                                          CCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTAC                                                   <  SRR4302535.4075213/94‑1 (MQ=60)
                                           CCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACA                                                  >  SRR4302535.2617959/1‑94 (MQ=60)
                                            CGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAA                                                 >  SRR4302535.3078952/1‑94 (MQ=60)
                                            CGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAA                                                 >  SRR4302535.4222496/1‑94 (MQ=60)
                                             GGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAAT                                                >  SRR4302535.918593/1‑94 (MQ=60)
                                               TCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCT                                              <  SRR4302535.233468/94‑1 (MQ=60)
                                               TCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCT                                              <  SRR4302535.3097156/94‑1 (MQ=60)
                                                CGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTG                                             <  SRR4302535.4060597/94‑1 (MQ=60)
                                                CGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCACTCGTGACCTACAATCTG                                             >  SRR4302535.4383265/1‑94 (MQ=60)
                                                 GGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGT                                            <  SRR4302535.4550124/94‑1 (MQ=60)
                                                    TCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAA                                         >  SRR4302535.4545131/1‑94 (MQ=60)
                                                     CAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAAC                                        <  SRR4302535.3573634/94‑1 (MQ=60)
                                                     CAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAAC                                        >  SRR4302535.2760975/1‑94 (MQ=60)
                                                     CAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAAC                                        >  SRR4302535.726284/1‑94 (MQ=60)
                                                     CAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAAC                                        <  SRR4302535.61365/94‑1 (MQ=60)
                                                      AACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACA                                       >  SRR4302535.1170313/1‑94 (MQ=60)
                                                      AACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACA                                       >  SRR4302535.4295326/1‑94 (MQ=60)
                                                       ACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAG                                      <  SRR4302535.2994610/94‑1 (MQ=60)
                                                         GTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAA                                    >  SRR4302535.1282746/1‑94 (MQ=60)
                                                         GTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAA                                    >  SRR4302535.1938032/1‑94 (MQ=60)
                                                         GTTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAA                                    >  SRR4302535.3874411/1‑94 (MQ=60)
                                                          TTATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAAT                                   <  SRR4302535.3245858/94‑1 (MQ=60)
                                                           TATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATG                                  <  SRR4302535.3486034/94‑1 (MQ=60)
                                                            ATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGT                                 <  SRR4302535.3478031/94‑1 (MQ=60)
                                                            ATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGT                                 >  SRR4302535.648497/1‑94 (MQ=60)
                                                            ATGAGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGT                                 <  SRR4302535.2053508/94‑1 (MQ=60)
                                                               AGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGCATGTGAA                              >  SRR4302535.2483846/1‑94 (MQ=60)
                                                               AGGTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAA                              >  SRR4302535.4455627/1‑94 (MQ=60)
                                                                 GTGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAA                            <  SRR4302535.16720/94‑1 (MQ=60)
                                                                  TGGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAAC                           <  SRR4302535.1049934/94‑1 (MQ=60)
                                                                   GGGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACG                          <  SRR4302535.1152812/94‑1 (MQ=60)
                                                                    GGGATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGT                         <  SRR4302535.3015518/94‑1 (MQ=60)
                                                                      GATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCA                       <  SRR4302535.2697566/94‑1 (MQ=60)
                                                                      GATGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCA                       <  SRR4302535.1363816/94‑1 (MQ=60)
                                                                        TGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAAT                     >  SRR4302535.3278671/1‑94 (MQ=60)
                                                                        TGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAAT                     <  SRR4302535.330417/94‑1 (MQ=60)
                                                                        TGCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAAT                     <  SRR4302535.3735762/94‑1 (MQ=60)
                                                                         GCCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATA                    <  SRR4302535.3165944/94‑1 (MQ=60)
                                                                          CCGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATAC                   <  SRR4302535.4591146/94‑1 (MQ=60)
                                                                           CGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAACTGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACA                  <  SRR4302535.2508400/94‑1 (MQ=60)
                                                                           CGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACA                  <  SRR4302535.4517778/94‑1 (MQ=60)
                                                                           CGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACA                  >  SRR4302535.3703895/1‑94 (MQ=60)
                                                                           CGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACA                  <  SRR4302535.3657413/94‑1 (MQ=60)
                                                                           CGTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACA                  <  SRR4302535.2820643/94‑1 (MQ=60)
                                                                            GTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAG                 >  SRR4302535.875159/1‑94 (MQ=60)
                                                                            GTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAG                 >  SRR4302535.3601348/1‑94 (MQ=60)
                                                                            GTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAG                 <  SRR4302535.2839359/94‑1 (MQ=60)
                                                                            GTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAG                 >  SRR4302535.712578/1‑94 (MQ=60)
                                                                            GTACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAG                 <  SRR4302535.335671/94‑1 (MQ=60)
                                                                              ACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCC               <  SRR4302535.1563516/94‑1 (MQ=60)
                                                                              ACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCC               >  SRR4302535.3311517/1‑94 (MQ=60)
                                                                              ACTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCC               >  SRR4302535.2661467/1‑94 (MQ=60)
                                                                               CTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCA              <  SRR4302535.788181/94‑1 (MQ=60)
                                                                               CTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCA              >  SRR4302535.4218975/1‑94 (MQ=60)
                                                                               CTAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCA              >  SRR4302535.670328/1‑94 (MQ=60)
                                                                                TAATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAA             <  SRR4302535.4617388/94‑1 (MQ=60)
                                                                                 AATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAC            <  SRR4302535.1585478/94‑1 (MQ=60)
                                                                                 AATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAAC            >  SRR4302535.2793759/1‑94 (MQ=60)
                                                                                  ATTAACATCAGCGTAACAGTGCGTATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACC           <  SRR4302535.1548213/94‑1 (MQ=60)
                                                                                  ATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACC           >  SRR4302535.3207331/1‑94 (MQ=60)
                                                                                  ATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACC           <  SRR4302535.4067837/94‑1 (MQ=60)
                                                                                  ATTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACC           >  SRR4302535.2441142/1‑94 (MQ=60)
                                                                                   TTAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCG          <  SRR4302535.1068621/94‑1 (MQ=60)
                                                                                    TAACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGG         <  SRR4302535.205016/94‑1 (MQ=60)
                                                                                     AACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAATCGGG        >  SRR4302535.741959/1‑94 (MQ=60)
                                                                                      ACATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGA       >  SRR4302535.4528071/1‑94 (MQ=60)
                                                                                       CATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGAT      <  SRR4302535.4535109/94‑1 (MQ=60)
                                                                                       CATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGAT      >  SRR4302535.1293035/1‑94 (MQ=60)
                                                                                        ATCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATT     >  SRR4302535.2563789/1‑94 (MQ=60)
                                                                                         TCAGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTT    <  SRR4302535.3718695/94‑1 (MQ=60)
                                                                                           AGCGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTAC  <  SRR4302535.1188897/94‑1 (MQ=60)
                                                                                             |                                                                                           
CTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTAC  >  NC_000913/3705371‑3705555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑