Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1873 183.1 9376148 98.2% 9207377 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,737,956 2 bp→CG coding (623‑624/1254 nt) avtA → alanine‑alpha‑ketoisovalerate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,737,9560GC100.0% 692.9 / NA 220G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (109/111);  total (109/111)
*NC_0009133,737,9570CG99.6% 695.2 / NA 220G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (109/110);  total (109/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 231 total aligned reads displayed.

AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043
                                                                                       ||                                                                                      
aaaCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATc                                                                                    >  2:859700/1‑93 (MQ=255)
  aCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGCTGCTGAAGCTTGACGCGCTGGCGAATCaa                                                                                  >  2:1808441/1‑93 (MQ=255)
   ccGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 >  2:4522966/1‑93 (MQ=255)
   ccGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 <  1:1772380/93‑1 (MQ=255)
   ccGGGATGATTTGCGCCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 <  1:4645428/93‑1 (MQ=255)
   acGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 <  1:159573/92‑1 (MQ=255)
    cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                <  2:530607/93‑1 (MQ=255)
    cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                <  1:1565658/93‑1 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacat                                                                               >  1:4542211/1‑92 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               >  2:103808/1‑93 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               <  2:2942999/93‑1 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               <  1:4372775/93‑1 (MQ=255)
     gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGAGCTGGCGAATCAacag                                                                               >  1:993035/1‑92 (MQ=255)
      ggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg                                                                              >  2:752556/1‑93 (MQ=255)
      ggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg                                                                              >  1:3452435/1‑93 (MQ=255)
        atgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc                                                                            <  2:4316514/93‑1 (MQ=255)
         tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           >  2:1146564/1‑93 (MQ=255)
         tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           <  1:2487088/93‑1 (MQ=255)
          gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt                                                                          >  1:3238869/1‑93 (MQ=255)
           atTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt                                                                         <  2:2639019/93‑1 (MQ=255)
            tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        <  2:3999876/93‑1 (MQ=255)
             ttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                       <  1:417998/93‑1 (MQ=255)
             ttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                       <  2:2185363/93‑1 (MQ=255)
               gCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGc                                                                     <  2:2577143/93‑1 (MQ=255)
                  tctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTgg                                                                  <  2:3668095/93‑1 (MQ=255)
                   ctcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTggg                                                                 >  2:4474938/1‑92 (MQ=255)
                    tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtg                                                                >  1:709347/1‑93 (MQ=255)
                      ccGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgat                                                              <  2:4148787/93‑1 (MQ=255)
                        ggCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATTAACACGGCATTCCGCTGGtgattg                                                            >  1:2671317/1‑93 (MQ=255)
                         gCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattga                                                           <  1:4074874/93‑1 (MQ=255)
                          ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          >  2:3383221/1‑93 (MQ=255)
                           cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         <  1:1130176/93‑1 (MQ=255)
                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTTGCGAATCAACACGGCATTCCGCTGGGGATTGATaa                                                        >  1:2345923/1‑93 (MQ=255)
                            gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa                                                        <  1:433936/93‑1 (MQ=255)
                              cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      >  1:3798435/1‑93 (MQ=255)
                               gaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGc                                                     >  2:1956256/1‑93 (MQ=255)
                                aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCt                                                    <  1:4481975/93‑1 (MQ=255)
                                  tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  >  2:1570961/1‑93 (MQ=255)
                                  tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  2:2650615/93‑1 (MQ=255)
                                  tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  >  1:4093152/1‑93 (MQ=255)
                                  tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  1:2440918/93‑1 (MQ=255)
                                   ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 <  2:3141213/93‑1 (MQ=255)
                                   ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAt                                                 <  1:2143614/93‑1 (MQ=255)
                                    cAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATg                                                <  2:2166621/93‑1 (MQ=255)
                                     aaCAGGCAATGTGATTACTGACTAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  1:1224641/93‑1 (MQ=255)
                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               >  1:3064079/1‑93 (MQ=255)
                                     aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  2:2476962/93‑1 (MQ=255)
                                      aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              <  1:2716873/93‑1 (MQ=255)
                                          gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc                                          >  1:114992/1‑93 (MQ=255)
                                           cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTccc                                         >  2:4646937/1‑93 (MQ=255)
                                            aaTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCg                                        <  2:4437377/93‑1 (MQ=255)
                                             aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt                                       >  1:475687/1‑93 (MQ=255)
                                               gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc                                     >  2:3954060/1‑93 (MQ=255)
                                               gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc                                     >  2:4058240/1‑93 (MQ=255)
                                               gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc                                     >  2:1487760/1‑93 (MQ=255)
                                               gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc                                     >  2:1072865/1‑93 (MQ=255)
                                               gtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTc                                     >  1:1692319/1‑93 (MQ=255)
                                                tgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTcc                                    >  2:327532/1‑93 (MQ=255)
                                                 gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTccc                                   >  1:3488661/1‑93 (MQ=255)
                                                   ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 <  1:1820158/93‑1 (MQ=255)
                                                   ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 >  2:3931639/1‑93 (MQ=255)
                                                   ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 <  2:2763622/93‑1 (MQ=255)
                                                     aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGt                               >  2:1223300/1‑93 (MQ=255)
                                                      cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  1:1496797/93‑1 (MQ=255)
                                                      cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              >  1:2869656/1‑93 (MQ=255)
                                                        gACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatc                            <  2:3420772/93‑1 (MQ=255)
                                                         aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatca                           <  2:3619630/93‑1 (MQ=255)
                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        >  2:4163804/1‑93 (MQ=255)
                                                            aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGCGTATCATCt                        >  2:3785387/1‑93 (MQ=255)
                                                              gagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCCTTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTc                      >  1:1030095/1‑93 (MQ=255)
                                                              gagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTc                      >  2:1293471/1‑93 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGGCGAATCAACTCGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    <  2:3875703/93‑1 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    <  2:4346562/93‑1 (MQ=255)
                                                                gTTGCTGAAGCTTGACGCGCTGCCGAATCAACACGCCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    <  2:3037674/93‑1 (MQ=255)
                                                                 ttGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGt                   <  2:1394985/93‑1 (MQ=255)
                                                                   gCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGa                 <  2:664848/93‑1 (MQ=255)
                                                                     tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGCCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               >  1:1934362/1‑93 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCATTGAAgc              >  2:3522010/1‑93 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  1:307721/1‑93 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  1:1292769/93‑1 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  1:224709/1‑93 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  2:2840680/1‑93 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  1:537478/93‑1 (MQ=255)
                                                                      gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  1:740224/1‑93 (MQ=255)
                                                                       aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             >  1:2989334/1‑93 (MQ=255)
                                                                       aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             >  1:3498195/1‑93 (MQ=255)
                                                                        aGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgc            >  2:2336438/1‑93 (MQ=255)
                                                                        aGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgc            <  1:1389570/93‑1 (MQ=255)
                                                                         gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  1:2560267/1‑93 (MQ=255)
                                                                         gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  2:3477710/1‑93 (MQ=255)
                                                                         gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           <  1:3730650/93‑1 (MQ=255)
                                                                         gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  2:3165927/1‑93 (MQ=255)
                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         <  2:425934/93‑1 (MQ=255)
                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         <  1:3534119/93‑1 (MQ=255)
                                                                           ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         >  1:3887433/1‑93 (MQ=255)
                                                                            tGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGccc        >  1:4433722/1‑93 (MQ=255)
                                                                               cgcgCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCt     <  1:2105947/93‑1 (MQ=255)
                                                                                gcgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGGTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTa    >  1:3271438/1‑93 (MQ=255)
                                                                                ccgcTGGCGAATCAACACGGCATTCCGCTGTTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTa    <  2:4466032/92‑1 (MQ=255)
                                                                                 cgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAt   >  1:3268166/1‑93 (MQ=255)
                                                                                       ||                                                                                      
AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737869‑3738043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737863‑3738047
                                                                                             |                                                                                            
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGC                                                                                              <  SRR4302535.283672/94‑1 (MQ=60)
       AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAA                                                                                      >  SRR4302535.1559399/1‑94 (MQ=60)
       AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAA                                                                                      >  SRR4302535.1323178/1‑94 (MQ=60)
        ACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAC                                                                                     <  SRR4302535.4240177/94‑1 (MQ=60)
         CCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    <  SRR4302535.1785223/94‑1 (MQ=60)
         CCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    >  SRR4302535.4337508/1‑94 (MQ=60)
         CCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    <  SRR4302535.1428171/94‑1 (MQ=60)
         CCGGGATGATTTGCGCCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    <  SRR4302535.4676907/94‑1 (MQ=60)
         ACGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    <  SRR4302535.160692/94‑1 (MQ=60)
          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAT                                                                                   >  SRR4302535.4573280/1‑94 (MQ=60)
          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAC                                                                                   <  SRR4302535.1068893/94‑1 (MQ=60)
          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAC                                                                                   <  SRR4302535.1576961/94‑1 (MQ=60)
          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGAGCTGGCG‑AATCAACAG                                                                                   >  SRR4302535.1000087/1‑94 (MQ=60)
           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  >  SRR4302535.3476432/1‑94 (MQ=60)
           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  <  SRR4302535.4402693/94‑1 (MQ=60)
           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  <  SRR4302535.3671232/94‑1 (MQ=60)
            GGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGG                                                                                 >  SRR4302535.79657/1‑94 (MQ=60)
             GATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGC                                                                                <  SRR4302535.1147350/94‑1 (MQ=60)
              ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               <  SRR4302535.2970923/94‑1 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              >  SRR4302535.3261356/1‑94 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302535.2505023/94‑1 (MQ=60)
                GATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATT                                                                             >  SRR4302535.2470586/1‑94 (MQ=60)
                GATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATT                                                                             >  SRR4302535.4522340/1‑94 (MQ=60)
                 ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            >  SRR4302535.849777/1‑94 (MQ=60)
                 AGTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            >  SRR4302535.4454450/1‑94 (MQ=60)
                  TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302535.3480335/94‑1 (MQ=60)
                   TTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCG                                                                          <  SRR4302535.420928/94‑1 (MQ=60)
                   TTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCG                                                                          >  SRR4302535.3254187/1‑94 (MQ=60)
                      CGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTG                                                                       <  SRR4302535.4131441/94‑1 (MQ=60)
                       GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGG                                                                      >  SRR4302535.3097181/1‑94 (MQ=60)
                       GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACCGCATTCCGCTGG                                                                      >  SRR4302535.3528562/1‑94 (MQ=60)
                         CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    >  SRR4302535.714326/1‑94 (MQ=60)
                            CCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATT                                                                 <  SRR4302535.3292596/94‑1 (MQ=60)
                             CGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATTAACACGGCATTCCGCTGGTGATTG                                                                >  SRR4302535.2690429/1‑94 (MQ=60)
                               GCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGAT                                                              <  SRR4302535.4102755/94‑1 (MQ=60)
                               GCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGAT                                                              <  SRR4302535.4472577/94‑1 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATTAACAAGGCATTCCGCTGGTGATTGATA                                                             >  SRR4302535.2156957/1‑94 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             <  SRR4302535.1573806/94‑1 (MQ=60)
                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTTGCG‑AATCAACACGGCATTCCGCTGGGGATTGATAA                                                            >  SRR4302535.2362735/1‑94 (MQ=60)
                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            <  SRR4302535.1138244/94‑1 (MQ=60)
                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302535.2207362/1‑94 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302535.3331594/94‑1 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302535.497360/94‑1 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302535.436958/94‑1 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302535.3802324/94‑1 (MQ=60)
                                   ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          >  SRR4302535.3824621/1‑94 (MQ=60)
                                      AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                       <  SRR4302535.4512737/94‑1 (MQ=60)
                                      AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                       >  SRR4302535.1210294/1‑94 (MQ=60)
                                       ATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTA                                                      >  SRR4302535.4121161/1‑94 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGTTAACGCTTAT                                                     <  SRR4302535.4115593/94‑1 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302535.2458503/94‑1 (MQ=60)
                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302535.4382580/94‑1 (MQ=60)
                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302535.2159033/94‑1 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302535.3085497/1‑94 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC‑CTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302535.1909205/94‑1 (MQ=60)
                                           AACAGGCAATGTGATTACTGACTAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302535.1233400/94‑1 (MQ=60)
                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302535.722464/94‑1 (MQ=60)
                                            ACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                                 <  SRR4302535.2736281/94‑1 (MQ=60)
                                              AGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTC                                               >  SRR4302535.2684523/1‑94 (MQ=60)
                                              AGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTC                                               >  SRR4302535.3516637/1‑94 (MQ=60)
                                               GGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCC                                              >  SRR4302535.115799/1‑94 (MQ=60)
                                               GGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCC                                              <  SRR4302535.479209/94‑1 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            <  SRR4302535.2755130/94‑1 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            <  SRR4302535.17908/94‑1 (MQ=60)
                                                  AATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                           >  SRR4302535.479023/1‑94 (MQ=60)
                                                    TGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTC                                         >  SRR4302535.1704560/1‑94 (MQ=60)
                                                     GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                        >  SRR4302535.3116686/1‑94 (MQ=60)
                                                     GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                        >  SRR4302535.4698923/1‑94 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       >  SRR4302535.3512881/1‑94 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       >  SRR4302535.3509018/1‑94 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       <  SRR4302535.2682680/94‑1 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       <  SRR4302535.4604495/94‑1 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       >  SRR4302535.2904438/1‑94 (MQ=60)
                                                       GATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCG                                      >  SRR4302535.4082939/1‑94 (MQ=60)
                                                        ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGG                                     <  SRR4302535.1730945/94‑1 (MQ=60)
                                                        ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGG                                     <  SRR4302535.2406796/94‑1 (MQ=60)
                                                        ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGG                                     >  SRR4302535.2692266/1‑94 (MQ=60)
                                                        ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGG                                     >  SRR4302535.2108847/1‑94 (MQ=60)
                                                         TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                    <  SRR4302535.4479529/94‑1 (MQ=60)
                                                         TTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGG                                    <  SRR4302535.1833307/94‑1 (MQ=60)
                                                           ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                  >  SRR4302535.1585986/1‑94 (MQ=60)
                                                           ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                  >  SRR4302535.2889978/1‑94 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 <  SRR4302535.1507561/94‑1 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 <  SRR4302535.1770276/94‑1 (MQ=60)
                                                              GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCA                               <  SRR4302535.3622341/94‑1 (MQ=60)
                                                                   AGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCCTTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTC                          >  SRR4302535.1037404/1‑94 (MQ=60)
                                                                   AGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTC                          >  SRR4302535.3072319/1‑94 (MQ=60)
                                                                   AGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGAATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTC                          >  SRR4302535.3289974/1‑94 (MQ=60)
                                                                    GAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA                         <  SRR4302535.3840737/94‑1 (MQ=60)
                                                                     AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        >  SRR4302535.2320639/1‑94 (MQ=60)
                                                                          CTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGCCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                   >  SRR4302535.1948293/1‑94 (MQ=60)
                                                                           TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  >  SRR4302535.745426/1‑94 (MQ=60)
                                                                           TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  >  SRR4302535.226257/1‑94 (MQ=60)
                                                                           TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  >  SRR4302535.309852/1‑94 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 >  SRR4302535.3010292/1‑94 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 >  SRR4302535.3522501/1‑94 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302535.541232/94‑1 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302535.1301981/94‑1 (MQ=60)
                                                                              AGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG               >  SRR4302535.2578699/1‑94 (MQ=60)
                                                                              AGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG               <  SRR4302535.1399487/94‑1 (MQ=60)
                                                                              AGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG               >  SRR4302535.68711/1‑94 (MQ=60)
                                                                               GCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGC              <  SRR4302535.3756484/94‑1 (MQ=60)
                                                                                CTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCC             >  SRR4302535.3914098/1‑94 (MQ=60)
                                                                                CTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCC             >  SRR4302535.2531565/1‑94 (MQ=60)
                                                                                 TTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCC            >  SRR4302535.4464102/1‑94 (MQ=60)
                                                                                 TTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCC            <  SRR4302535.3558655/94‑1 (MQ=60)
                                                                                  TGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG           >  SRR4302535.2410149/1‑94 (MQ=60)
                                                                                  TGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCG           >  SRR4302535.4043725/1‑94 (MQ=60)
                                                                                     CGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTA        <  SRR4302535.1254439/94‑1 (MQ=60)
                                                                                     CGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTA        >  SRR4302535.1661121/1‑94 (MQ=60)
                                                                                     CGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTA        >  SRR4302535.2068292/1‑94 (MQ=60)
                                                                                     CGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTA        <  SRR4302535.2121139/94‑1 (MQ=60)
                                                                                     CGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGGTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTA        >  SRR4302535.3294163/1‑94 (MQ=60)
                                                                                      GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       >  SRR4302535.3290870/1‑94 (MQ=60)
                                                                                        GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG     <  SRR4302535.1239700/94‑1 (MQ=60)
                                                                                        GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCACTATGG     >  SRR4302535.3613254/1‑94 (MQ=60)
                                                                                         CTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA    >  SRR4302535.2782577/1‑94 (MQ=60)
                                                                                            GGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  <  SRR4302535.276193/94‑1 (MQ=60)
                                                                                             |                                                                                            
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737863‑3738047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑