Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1696 279.3 15874187 97.8% 15524954 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,737,956 2 bp→CG coding (623‑624/1254 nt) avtA → alanine‑alpha‑ketoisovalerate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,737,9560GC100.0% 1245.5 / NA 395G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (188/207);  total (188/207)
*NC_0009133,737,9570CG99.8% 1262.2 / ‑5.5 396G208A (GGC→GCG) avtAalanine‑alpha‑ketoisovalerate transaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (187/208);  minor base A (1/0);  total (188/208)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 410 total aligned reads displayed.

GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737868‑3738043
                                                                                        ||                                                                                      
  aaCCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCa                                                                                   >  1:1586338/1‑93 (MQ=255)
    ccGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAac                                                                                 <  1:2589885/93‑1 (MQ=255)
     cGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAaca                                                                                <  2:293903/93‑1 (MQ=255)
      gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               <  2:372502/93‑1 (MQ=255)
      gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               >  2:4815324/1‑93 (MQ=255)
      gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               >  2:6568574/1‑93 (MQ=255)
      gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAacac                                                                               >  2:5385889/1‑93 (MQ=255)
      gggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATAAacac                                                                               >  1:2396943/1‑93 (MQ=255)
       ggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg                                                                              <  2:1701774/93‑1 (MQ=255)
       ggATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACg                                                                              >  2:2242038/1‑93 (MQ=255)
         atgttTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGc                                                                            <  2:665141/93‑1 (MQ=255)
          tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           <  1:7611921/93‑1 (MQ=255)
          tgatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCa                                                                           >  1:4282729/1‑93 (MQ=255)
           gatTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAt                                                                          <  2:3435763/93‑1 (MQ=255)
            atTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt                                                                         >  2:310344/1‑93 (MQ=255)
            atTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCAtt                                                                         <  2:7288916/93‑1 (MQ=255)
             tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        <  2:4925143/93‑1 (MQ=255)
             tttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTc                                                                        <  1:3910173/93‑1 (MQ=255)
              ttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                       <  1:4773165/93‑1 (MQ=255)
              ttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                       >  2:2561588/1‑93 (MQ=255)
              ttGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTcc                                                                       <  1:1012376/93‑1 (MQ=255)
                  gTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCGTGCCGCGCTGGCGAATCAACACGGCATTCCGCTg                                                                   <  1:4860416/93‑1 (MQ=255)
                   tctcCCGGCCGACGAATCCAACAGGCAATGTAATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTgg                                                                  <  1:691459/93‑1 (MQ=255)
                     tcCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtg                                                                >  1:6333462/1‑93 (MQ=255)
                        cGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgatt                                                             <  2:5788533/93‑1 (MQ=255)
                         ggCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattg                                                            >  1:5675966/1‑93 (MQ=255)
                           ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          >  1:4649609/1‑93 (MQ=255)
                           ccGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGtgattgat                                                          <  2:103125/93‑1 (MQ=255)
                            cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  1:4335048/1‑93 (MQ=255)
                            cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         <  1:4362461/93‑1 (MQ=255)
                            cgacgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATa                                                         >  1:1436481/1‑93 (MQ=255)
                             gacgaATCCAACAGGCAATGTGATTACTGACGAAGAGATGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATaa                                                        >  1:3344042/1‑93 (MQ=255)
                              acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  1:1549434/93‑1 (MQ=255)
                              acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  1:7427487/93‑1 (MQ=255)
                              acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       <  2:2424162/93‑1 (MQ=255)
                              acgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAAc                                                       >  2:1374191/1‑93 (MQ=255)
                               cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      >  2:3309813/1‑93 (MQ=255)
                               cgaATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACg                                                      <  1:1197018/93‑1 (MQ=255)
                                 aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCt                                                    <  1:6590012/93‑1 (MQ=255)
                                 aaTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCt                                                    >  2:3989949/1‑93 (MQ=255)
                                  aTCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCtt                                                   >  1:2819322/1‑93 (MQ=255)
                                   tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  >  2:3848157/1‑93 (MQ=255)
                                   tCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTa                                                  <  1:6012212/93‑1 (MQ=255)
                                    ccAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATACCGCTGGTGATTGATAACGCTTAt                                                 <  2:7589081/93‑1 (MQ=255)
                                      aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  2:5059238/93‑1 (MQ=255)
                                      aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  2:3795886/93‑1 (MQ=255)
                                      aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               >  1:2088370/1‑93 (MQ=255)
                                      aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               >  1:7905396/1‑93 (MQ=255)
                                      aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  1:2274777/93‑1 (MQ=255)
                                      aaCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATgg                                               <  1:3691484/93‑1 (MQ=255)
                                       aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              >  1:1715134/1‑93 (MQ=255)
                                       aCAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGc                                              <  2:7213310/93‑1 (MQ=255)
                                        cAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCg                                             >  1:893260/1‑93 (MQ=255)
                                           gCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTcc                                          <  2:7806003/93‑1 (MQ=255)
                                            cAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTccc                                         <  1:3268826/93‑1 (MQ=255)
                                              aTGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGt                                       <  1:503859/93‑1 (MQ=255)
                                               tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      >  2:4437564/1‑93 (MQ=255)
                                               tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      >  1:275792/1‑93 (MQ=255)
                                               tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      >  1:3125463/1‑93 (MQ=255)
                                               tgtgATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGtt                                      >  2:5630302/1‑93 (MQ=255)
                                                  gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTccc                                   <  2:3636632/93‑1 (MQ=255)
                                                  gATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTccc                                   >  1:5851029/1‑93 (MQ=255)
                                                    ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCgg                                 >  2:5925180/1‑93 (MQ=255)
                                                    ttACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCATATGGCGTCCCGTTCCCgg                                 >  1:7048025/1‑93 (MQ=255)
                                                     tACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCggg                                <  2:3525420/93‑1 (MQ=255)
                                                      aCTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGt                               <  2:1826238/93‑1 (MQ=255)
                                                       cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGtc                              >  1:1112076/1‑92 (MQ=255)
                                                       cTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTa                              <  1:4158712/93‑1 (MQ=255)
                                                         gACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatc                            >  2:6863072/1‑93 (MQ=255)
                                                         gACGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCCGTTatt                            >  1:1862265/1‑92 (MQ=255)
                                                          aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatca                           <  2:2738898/93‑1 (MQ=255)
                                                          aCGAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatca                           <  2:4042475/93‑1 (MQ=255)
                                                            gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         <  2:5240801/93‑1 (MQ=255)
                                                            gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTatcatc                         >  2:4858034/1‑93 (MQ=255)
                                                            gAAGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGCGATTGATAACGCTTATGGCGTCCCGTTGCCGGGTgtcatc                         >  2:6107008/1‑93 (MQ=255)
                                                             aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        <  1:7488792/93‑1 (MQ=255)
                                                             aaGAGTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCt                        <  2:1135579/93‑1 (MQ=255)
                                                              agagTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCtt                       >  2:1436685/1‑93 (MQ=255)
                                                                 gTTGCTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAg                    >  2:2871381/1‑93 (MQ=255)
                                                                     cTGAAGCTTGACGCTCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGaa                <  1:6788289/93‑1 (MQ=255)
                                                                     cTGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGaa                <  2:2029976/93‑1 (MQ=255)
                                                                      tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               <  1:3290167/93‑1 (MQ=255)
                                                                      tGAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAg               <  1:3546624/93‑1 (MQ=255)
                                                                       gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  1:1363639/93‑1 (MQ=255)
                                                                       gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  1:4478655/93‑1 (MQ=255)
                                                                       gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              >  1:522255/1‑93 (MQ=255)
                                                                       gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgc              <  2:4220315/93‑1 (MQ=255)
                                                                       gAAGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAga              <  1:2961497/93‑2 (MQ=255)
                                                                        aaGCTTGACGCGCTGGCGAATCAACGCGGCATTCCTTTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             <  1:4092880/93‑1 (MQ=255)
                                                                        aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             <  2:5568924/93‑1 (MQ=255)
                                                                        aaGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcg             >  2:7826945/1‑93 (MQ=255)
                                                                         aGCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgc            >  1:5489964/1‑93 (MQ=255)
                                                                          gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  1:3740143/1‑93 (MQ=255)
                                                                          gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  2:6248030/1‑93 (MQ=255)
                                                                          gCTTGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAgcgcg           >  1:5550558/1‑93 (MQ=255)
                                                                            ttGACGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGcc         >  1:7282034/1‑93 (MQ=255)
                                                                               aCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGc      <  1:7793050/93‑1 (MQ=255)
                                                                               aCGCGCTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGc      <  1:7170532/93‑1 (MQ=255)
                                                                                 gcgcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTa    <  2:7071606/93‑1 (MQ=255)
                                                                                   gcTGGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATg  <  2:7931310/93‑1 (MQ=255)
                                                                                        ||                                                                                      
GAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGCAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG  >  NC_000913/3737868‑3738043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 224 total aligned reads displayed.
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737863‑3738047
                                                                                             |                                                                                            
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGC                                                                                              <  SRR4302543.1479573/94‑1 (MQ=60)
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGC                                                                                              <  SRR4302543.3870295/94‑1 (MQ=60)
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGC                                                                                              <  SRR4302543.5223692/94‑1 (MQ=60)
      AAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCA                                                                                       <  SRR4302543.4469654/94‑1 (MQ=60)
       AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAA                                                                                      >  SRR4302543.3209701/1‑94 (MQ=60)
       AACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAA                                                                                      <  SRR4302543.3448326/94‑1 (MQ=60)
        ACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAC                                                                                     <  SRR4302543.3923802/94‑1 (MQ=60)
        ACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAC                                                                                     >  SRR4302543.2442437/1‑94 (MQ=60)
         CCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    >  SRR4302543.7576099/1‑94 (MQ=60)
         CCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACA                                                                                    <  SRR4302543.2613429/94‑1 (MQ=60)
          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACAC                                                                                   <  SRR4302543.1828344/94‑1 (MQ=60)
          CGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATAAACAC                                                                                   >  SRR4302543.2418627/1‑94 (MQ=60)
           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  >  SRR4302543.7345026/1‑94 (MQ=60)
           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  <  SRR4302543.471064/94‑1 (MQ=60)
           GGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACG                                                                                  >  SRR4302543.1063733/1‑94 (MQ=60)
            GGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGG                                                                                 <  SRR4302543.6690249/94‑1 (MQ=60)
            GGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGG                                                                                 >  SRR4302543.6750427/1‑94 (MQ=60)
              ATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCA                                                                               >  SRR4302543.4321657/1‑94 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302543.2589076/94‑1 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              >  SRR4302543.4420921/1‑94 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302543.1567757/94‑1 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302543.4415027/94‑1 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302543.7228962/94‑1 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302543.7684193/94‑1 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302543.3187319/94‑1 (MQ=60)
               TGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302543.287079/94‑1 (MQ=60)
               TGATTTGCGCCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCAT                                                                              <  SRR4302543.1267741/94‑1 (MQ=60)
                GATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATT                                                                             <  SRR4302543.1482873/94‑1 (MQ=60)
                GATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATT                                                                             >  SRR4302543.2309812/1‑94 (MQ=60)
                GATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATT                                                                             <  SRR4302543.7414820/94‑1 (MQ=60)
                GATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATT                                                                             <  SRR4302543.4620911/94‑1 (MQ=60)
                 ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            >  SRR4302543.3723899/1‑94 (MQ=60)
                 ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            <  SRR4302543.4264840/94‑1 (MQ=60)
                 ATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTC                                                                            >  SRR4302543.4400409/1‑94 (MQ=60)
                  TTTGCGTCTCCCGGCCGCCGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302543.7092336/94‑1 (MQ=60)
                  TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302543.6880060/94‑1 (MQ=60)
                  TTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCC                                                                           <  SRR4302543.3944692/94‑1 (MQ=60)
                   TTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCG                                                                          <  SRR4302543.1021331/94‑1 (MQ=60)
                   TTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCG                                                                          <  SRR4302543.4816788/94‑1 (MQ=60)
                     GCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCT                                                                        <  SRR4302543.1397067/94‑1 (MQ=60)
                      CGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTG                                                                       >  SRR4302543.7848278/1‑94 (MQ=60)
                      CGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTG                                                                       >  SRR4302543.1586645/1‑94 (MQ=60)
                       GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGATGCGCTGGCG‑CATCAACACGGCATTCCGCTGG                                                                      >  SRR4302543.1600952/1‑94 (MQ=60)
                       GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGG                                                                      >  SRR4302543.7319274/1‑94 (MQ=60)
                       GTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCGTGCCGCGCTGGCG‑AATCAACACGGCATTCCGCTGG                                                                      <  SRR4302543.4904735/94‑1 (MQ=60)
                        TCTCCCGGCCGACGAATCCAACAGGCAATGTAATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGT                                                                     <  SRR4302543.697801/94‑1 (MQ=60)
                         CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    >  SRR4302543.1314060/1‑94 (MQ=60)
                         CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    >  SRR4302543.6393026/1‑94 (MQ=60)
                         CTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTG                                                                    <  SRR4302543.1476792/94‑1 (MQ=60)
                          TCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGA                                                                   >  SRR4302543.7870993/1‑94 (MQ=60)
                            CCGGCCGATGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATT                                                                 >  SRR4302543.2458620/1‑94 (MQ=60)
                             CGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTG                                                                <  SRR4302543.3875843/94‑1 (MQ=60)
                              GGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGA                                                               >  SRR4302543.4663850/1‑94 (MQ=60)
                               GCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGAT                                                              <  SRR4302543.5320724/94‑1 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             <  SRR4302543.1028167/94‑1 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             <  SRR4302543.4893184/94‑1 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             >  SRR4302543.4374370/1‑94 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             >  SRR4302543.1449466/1‑94 (MQ=60)
                                CCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATA                                                             >  SRR4302543.1383054/1‑94 (MQ=60)
                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302543.263181/1‑94 (MQ=60)
                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            <  SRR4302543.4401983/94‑1 (MQ=60)
                                 CGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGATGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAA                                                            >  SRR4302543.3374153/1‑94 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302543.2084424/94‑1 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           <  SRR4302543.4807057/94‑1 (MQ=60)
                                  GACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAAC                                                           >  SRR4302543.3313357/1‑94 (MQ=60)
                                   ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          <  SRR4302543.3770939/94‑1 (MQ=60)
                                   ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          <  SRR4302543.7498705/94‑1 (MQ=60)
                                   ACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACG                                                          <  SRR4302543.1563289/94‑1 (MQ=60)
                                    CGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGC                                                         <  SRR4302543.1207800/94‑1 (MQ=60)
                                    CGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGC                                                         >  SRR4302543.7658908/1‑94 (MQ=60)
                                     GAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCT                                                        <  SRR4302543.2869722/94‑1 (MQ=60)
                                      AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                       >  SRR4302543.2845125/1‑94 (MQ=60)
                                      AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                       <  SRR4302543.7541805/94‑1 (MQ=60)
                                      AATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTT                                                       <  SRR4302543.6652098/94‑1 (MQ=60)
                                       ATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTA                                                      >  SRR4302543.3083046/1‑94 (MQ=60)
                                       ATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTA                                                      >  SRR4302543.1153612/1‑94 (MQ=60)
                                       ATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTA                                                      >  SRR4302543.7912084/1‑94 (MQ=60)
                                       ATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCACTGGTGATTGATAACGCTTA                                                      <  SRR4302543.1656831/94‑1 (MQ=60)
                                       ATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCACTGGTGATTGATAACGCTTA                                                      <  SRR4302543.6348099/94‑1 (MQ=60)
                                       ATCCAACAGGCAATGCGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTA                                                      >  SRR4302543.5579073/1‑94 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302543.6435800/94‑1 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302543.571830/94‑1 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302543.6067712/94‑1 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302543.7725274/94‑1 (MQ=60)
                                        TCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302543.2381676/94‑1 (MQ=60)
                                        ACCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302543.438747/94‑1 (MQ=60)
                                        ACCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTAT                                                     <  SRR4302543.1608660/94‑1 (MQ=60)
                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302543.6595985/94‑1 (MQ=60)
                                         CCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATG                                                    <  SRR4302543.5878147/94‑1 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGTTTGATAACGCTTATGG                                                   >  SRR4302543.7114353/1‑94 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302543.2107029/1‑94 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302543.7979684/1‑94 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302543.7625949/1‑94 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   <  SRR4302543.3665665/94‑1 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302543.2801322/1‑94 (MQ=60)
                                          CAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGAATTCCGCTGGTGATTGATAACGCTTATGG                                                   >  SRR4302543.159472/1‑94 (MQ=60)
                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAAGACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302543.1403810/94‑1 (MQ=60)
                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302543.84875/94‑1 (MQ=60)
                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302543.2295580/94‑1 (MQ=60)
                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  >  SRR4302543.1730463/1‑94 (MQ=60)
                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302543.3723925/94‑1 (MQ=60)
                                           AACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302543.3503529/94‑1 (MQ=60)
                                           AACAGGCAATGTGAGTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGC                                                  <  SRR4302543.2295579/94‑1 (MQ=60)
                                            ACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCG                                                 >  SRR4302543.901151/1‑94 (MQ=60)
                                             CAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                                <  SRR4302543.3336309/94‑1 (MQ=60)
                                             CAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                                <  SRR4302543.1866132/94‑1 (MQ=60)
                                             CAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                                >  SRR4302543.7994921/1‑94 (MQ=60)
                                             CAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGT                                                >  SRR4302543.3835276/1‑94 (MQ=60)
                                              AGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTC                                               >  SRR4302543.1339840/1‑94 (MQ=60)
                                              AGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTC                                               >  SRR4302543.3361588/1‑94 (MQ=60)
                                               GGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCC                                              >  SRR4302543.3988538/1‑94 (MQ=60)
                                                GCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCC                                             <  SRR4302543.5930448/94‑1 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            >  SRR4302543.3517253/1‑94 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            >  SRR4302543.7552217/1‑94 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            <  SRR4302543.4399501/94‑1 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            >  SRR4302543.3746692/1‑94 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            <  SRR4302543.3298361/94‑1 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            >  SRR4302543.3965694/1‑94 (MQ=60)
                                                 CAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCG                                            >  SRR4302543.4867890/1‑94 (MQ=60)
                                                  AATGTGATTACTGACGTAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTACGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                           >  SRR4302543.7727512/1‑94 (MQ=60)
                                                  AATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                           <  SRR4302543.7921493/94‑1 (MQ=60)
                                                  AATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGT                                           >  SRR4302543.2821207/1‑94 (MQ=60)
                                                   ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTT                                          >  SRR4302543.3153578/1‑94 (MQ=60)
                                                   ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTT                                          >  SRR4302543.278227/1‑94 (MQ=60)
                                                   ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTT                                          <  SRR4302543.508537/94‑1 (MQ=60)
                                                   ATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGCGATTGATAACGCTTATGGCGTCCCGTC                                          >  SRR4302543.5553597/1‑94 (MQ=60)
                                                    TGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTC                                         <  SRR4302543.5668867/94‑1 (MQ=60)
                                                    TGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTC                                         <  SRR4302543.3641208/94‑1 (MQ=60)
                                                     GTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCC                                        <  SRR4302543.1472737/94‑1 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       <  SRR4302543.556838/94‑1 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       >  SRR4302543.3309551/1‑94 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       <  SRR4302543.4583836/94‑1 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       >  SRR4302543.5904270/1‑94 (MQ=60)
                                                      TGATTACTGACGAAGAGTTGCTGAAGCTTGACGAGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCC                                       <  SRR4302543.4583838/94‑1 (MQ=60)
                                                       GATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCG                                      <  SRR4302543.5043948/94‑1 (MQ=60)
                                                        ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGG                                     <  SRR4302543.6466673/94‑1 (MQ=60)
                                                        ATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCATATGGCGTCCCGTTCCCGG                                     >  SRR4302543.7116372/1‑94 (MQ=60)
                                                           ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTC                                  >  SRR4302543.1122125/1‑94 (MQ=60)
                                                           ACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTA                                  >  SRR4302543.719579/1‑94 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 >  SRR4302543.5871114/1‑94 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 <  SRR4302543.2182018/94‑1 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 >  SRR4302543.4137308/1‑94 (MQ=60)
                                                            CTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTAT                                 <  SRR4302543.4196331/94‑1 (MQ=60)
                                                             TGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCcgttatt                                >  SRR4302543.1878994/1‑87 (MQ=60)
                                                             TGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATC                                >  SRR4302543.4352122/1‑94 (MQ=60)
                                                             TGACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATC                                >  SRR4302543.6039047/1‑94 (MQ=60)
                                                              GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGTTTATGGCGTCCCGTTCCCGGGTATCA                               <  SRR4302543.1153916/94‑1 (MQ=60)
                                                              GACGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCA                               >  SRR4302543.4837287/1‑94 (MQ=60)
                                                                CGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATC                             <  SRR4302543.5148947/94‑1 (MQ=60)
                                                                CGAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATC                             >  SRR4302543.3135925/1‑94 (MQ=60)
                                                                 GAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCT                            <  SRR4302543.326502/94‑1 (MQ=60)
                                                                 GAAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCT                            >  SRR4302543.3418074/1‑94 (MQ=60)
                                                                  AAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTT                           >  SRR4302543.4332618/1‑94 (MQ=60)
                                                                  AAGAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTT                           >  SRR4302543.1200535/1‑94 (MQ=60)
                                                                    GAGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCA                         <  SRR4302543.2965862/94‑1 (MQ=60)
                                                                     AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTCTCATCTTCAG                        >  SRR4302543.1061162/1‑94 (MQ=60)
                                                                     AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        <  SRR4302543.2269641/94‑1 (MQ=60)
                                                                     AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        >  SRR4302543.6967018/1‑94 (MQ=60)
                                                                     AGTTGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAG                        >  SRR4302543.7806846/1‑94 (MQ=60)
                                                                        TGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGA                     >  SRR4302543.5950676/1‑94 (MQ=60)
                                                                        TGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGA                     >  SRR4302543.578806/1‑94 (MQ=60)
                                                                        TGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGA                     <  SRR4302543.720913/94‑1 (MQ=60)
                                                                        TGCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGA                     <  SRR4302543.5142413/94‑1 (MQ=60)
                                                                         GCTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAA                    <  SRR4302543.4191403/94‑1 (MQ=60)
                                                                          CTGAAGCTTGACGCTCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                   <  SRR4302543.6852396/94‑1 (MQ=60)
                                                                          CTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                   >  SRR4302543.3370938/1‑94 (MQ=60)
                                                                          CTGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAG                   >  SRR4302543.1966876/1‑94 (MQ=60)
                                                                           TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  <  SRR4302543.3319864/94‑1 (MQ=60)
                                                                           TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  <  SRR4302543.4326377/94‑1 (MQ=60)
                                                                           TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  >  SRR4302543.7535770/1‑94 (MQ=60)
                                                                           TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  >  SRR4302543.527096/1‑94 (MQ=60)
                                                                           TGAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGC                  <  SRR4302543.3578110/94‑1 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302543.1375857/94‑1 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302543.1841539/94‑1 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302543.280317/94‑1 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302543.4145162/94‑1 (MQ=60)
                                                                            GAAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCG                 <  SRR4302543.4519322/94‑1 (MQ=60)
                                                                             AAGCTTGACGCGCTGGCG‑AATCAACGCGGCATTCCTTTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGC                <  SRR4302543.4129595/94‑1 (MQ=60)
                                                                             AAGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGC                >  SRR4302543.5539751/1‑94 (MQ=60)
                                                                              AGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG               >  SRR4302543.3773046/1‑94 (MQ=60)
                                                                              AGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG               >  SRR4302543.6955456/1‑94 (MQ=60)
                                                                              AGCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCG               >  SRR4302543.5601037/1‑94 (MQ=60)
                                                                               GCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGC              >  SRR4302543.4294276/1‑94 (MQ=60)
                                                                               GCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGC              >  SRR4302543.5618481/1‑94 (MQ=60)
                                                                               GCTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGAGCGC              >  SRR4302543.2189453/1‑94 (MQ=60)
                                                                                CTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCC             >  SRR4302543.3058269/1‑94 (MQ=60)
                                                                                CTTGACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCC             >  SRR4302543.7352355/1‑94 (MQ=60)
                                                                                   GACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGC          >  SRR4302543.7491034/1‑94 (MQ=60)
                                                                                   GACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGC          >  SRR4302543.7424725/1‑94 (MQ=60)
                                                                                   GACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGC          >  SRR4302543.826848/1‑94 (MQ=60)
                                                                                    ACGCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCT         <  SRR4302543.7240162/94‑1 (MQ=60)
                                                                                      GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       <  SRR4302543.5134083/94‑1 (MQ=60)
                                                                                      GCGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTAT       <  SRR4302543.6254026/94‑1 (MQ=60)
                                                                                       CGCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATG      <  SRR4302543.5392785/94‑1 (MQ=60)
                                                                                        GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG     <  SRR4302543.4815992/94‑1 (MQ=60)
                                                                                        GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG     <  SRR4302543.2760940/94‑1 (MQ=60)
                                                                                        GCTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGG     <  SRR4302543.6329098/94‑1 (MQ=60)
                                                                                         CTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGA    >  SRR4302543.2057460/1‑94 (MQ=60)
                                                                                         CTGGCG‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGCATCATCTTCAGTGAAGCGCGCCCGCTATGGA    >  SRR4302543.7030815/1‑94 (MQ=60)
                                                                                            GGCGAATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  <  SRR4302543.6959069/94‑1 (MQ=60)
                                                                                             |                                                                                            
GCGAAGAAACCGGGATGATTTGCGTCTCCCGGCCGACGAATCCAACAGGCAATGTGATTACTGACGAAGAGTTGCTGAAGCTTGACGCGCTGGGC‑AATCAACACGGCATTCCGCTGGTGATTGATAACGCTTATGGCGTCCCGTTCCCGGGTATCATCTTCAGTGAAGCGCGCCCGCTATGGAAT  >  NC_000913/3737863‑3738047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑