Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1873	183.1	9376148	98.2%	9207377	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,793,037	A→G	Q161R (CAG→CGG)	rfaF →	ADP‑heptose‑‑lps heptosyltransferase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,793,037	0	A	G	99.6%	870.2 / NA	274	Q161R (CAG→CGG)	rfaF	ADP‑heptose‑‑lps heptosyltransferase II
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (117/156); minor base C (1/0); total (118/156)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 289 total aligned reads displayed.

TGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGT  >  NC_000913/3792947‑3793126

tggtggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCggg                                                                                         >  2:299491/1‑93 (MQ=255)

tggtggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCggg                                                                                         >  2:557589/1‑93 (MQ=255)

 ggtggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGt                                                                                        <  1:1419043/93‑1 (MQ=255)

    ggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAg                                                                                     >  1:3356455/1‑93 (MQ=255)

    ggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAg                                                                                     >  1:2869123/1‑93 (MQ=255)

    ggAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAg                                                                                     >  1:2165756/1‑93 (MQ=255)

     gAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGc                                                                                    <  2:2931949/93‑1 (MQ=255)

      aaCGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGca                                                                                   >  2:1983172/1‑92 (MQ=255)

        cGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGaa                                                                                 <  1:3719086/93‑1 (MQ=255)

         gCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAg                                                                                <  1:2280978/93‑1 (MQ=255)

          cTATATAGCGCTGGCCTATGACAAAGGCATTAAGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAgg                                                                               <  2:463601/93‑1 (MQ=255)

          cTATATAGCGCTGGCCTATGACAAAGCCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAgg                                                                               <  2:1907763/93‑1 (MQ=255)

           tatataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGt                                                                              <  2:2849347/93‑1 (MQ=255)

            atataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTg                                                                             >  2:2233129/1‑93 (MQ=255)

            atataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTg                                                                             >  2:23536/1‑93 (MQ=255)

             tataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGa                                                                            <  1:1099907/93‑1 (MQ=255)

             tataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGa                                                                            <  1:1663350/93‑1 (MQ=255)

              ataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaa                                                                           >  1:1164183/1‑93 (MQ=255)

              ataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaa                                                                           >  2:3082930/1‑93 (MQ=255)

              ataGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaa                                                                           <  1:3277937/93‑1 (MQ=255)

               taGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaa                                                                          >  2:3426504/1‑93 (MQ=255)

               taGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaa                                                                          <  1:1711055/93‑1 (MQ=255)

                aGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaaa                                                                         <  1:19024/93‑1 (MQ=255)

                aGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaaa                                                                         <  1:593840/93‑1 (MQ=255)

                aGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaaa                                                                         <  2:3847004/93‑1 (MQ=255)

                 gcgcTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaaaa                                                                        >  1:3237142/1‑93 (MQ=255)

                 gcgcTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGaaaaa                                                                        <  1:4508422/93‑1 (MQ=255)

                  cgcTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAAt                                                                       <  1:150187/93‑1 (MQ=255)

                   gcTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATc                                                                      <  2:869999/93‑1 (MQ=255)

                    cTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCa                                                                     <  1:2002110/93‑1 (MQ=255)

                     tGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGAGAGCGAAGGTGAAAaataat                                                                    >  1:935723/1‑93 (MQ=255)

                       gCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCatat                                                                  <  2:2675069/93‑1 (MQ=255)

                       gCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCatat                                                                  <  1:741130/93‑1 (MQ=255)

                        ccTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCAtata                                                                 <  2:2586665/93‑1 (MQ=255)

                        ccTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCAtata                                                                 <  2:460898/93‑1 (MQ=255)

                        ccTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAAACAtata                                                                 >  2:2462103/1‑93 (MQ=255)

                          tATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATAcc                                                               <  2:3468936/93‑1 (MQ=255)

                          tATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATAcc                                                               >  2:307142/1‑93 (MQ=255)

                          tATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATAcc                                                               <  2:3604139/93‑1 (MQ=255)

                          tATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATAcc                                                               <  1:3641652/93‑1 (MQ=255)

                          tATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGCTTCGGGTGAGCGAAGGTGAAAAGTCATATAcc                                                               >  2:1190082/1‑93 (MQ=255)

                            tGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTg                                                             >  1:3845942/1‑93 (MQ=255)

                            tGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTg                                                             <  2:3571143/93‑1 (MQ=255)

                              aCAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTa                                                           >  1:3933896/1‑93 (MQ=255)

                              aCAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTa                                                           >  1:2725489/1‑93 (MQ=255)

                               cAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTaa                                                          >  2:3896089/1‑93 (MQ=255)

                               cAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTaa                                                          <  2:1630620/93‑1 (MQ=255)

                                 aaGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGGAAAATCATATACCTGTAAtt                                                        >  2:1720024/1‑92 (MQ=255)

                                 aaGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATc                                                        <  2:4135590/93‑1 (MQ=255)

                                  aGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCa                                                       >  2:3948070/1‑93 (MQ=255)

                                   ggCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCaa                                                      <  2:3692941/93‑1 (MQ=255)

                                   ggCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCaa                                                      >  1:301612/1‑93 (MQ=255)

                                    gCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAt                                                     <  2:3813323/93‑1 (MQ=255)

                                     cATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAtt                                                    <  2:805913/93‑1 (MQ=255)

                                      aTTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAttt                                                   >  2:1119787/1‑93 (MQ=255)

                                      aTTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAttt                                                   <  2:949392/93‑1 (MQ=255)

                                        tATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCCGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTc                                                 >  1:1194248/1‑93 (MQ=255)

                                          tGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGc                                               <  1:4391772/93‑1 (MQ=255)

                                            cgcACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCtt                                             <  2:638938/93‑1 (MQ=255)

                                            cgcACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCtt                                             <  2:1754572/93‑1 (MQ=255)

                                            cgcACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCtt                                             <  2:1845614/93‑1 (MQ=255)

                                            cgcACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCtt                                             <  1:1853078/93‑1 (MQ=255)

                                             gcacagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCCGTTGCGGGTGAGCGAAGGTGGAAACTCATATACCTGTAATCAATTTTCGCttt                                            >  2:1006337/1‑93 (MQ=255)

                                             gcacagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCttt                                            >  1:2063159/1‑93 (MQ=255)

                                              cacagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtc                                           >  1:352799/1‑93 (MQ=255)

                                              cacagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtc                                           <  1:498566/93‑1 (MQ=255)

                                               acagcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtca                                          <  1:1278051/93‑1 (MQ=255)

                                                    acaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGa                                     >  2:1624696/1‑93 (MQ=255)

                                                     caAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGaa                                    <  1:2398630/93‑1 (MQ=255)

                                                       aGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACg                                  <  1:4164608/93‑1 (MQ=255)

                                                       aGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACg                                  >  1:2652428/1‑93 (MQ=255)

                                                        aaccTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGt                                 <  2:720156/90‑1 (MQ=255)

                                                          tCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTcc                               >  1:491648/1‑93 (MQ=255)

                                                            tGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCga                             <  2:2756015/93‑1 (MQ=255)

                                                            tGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCga                             <  1:2496505/93‑1 (MQ=255)

                                                            tGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCga                             <  2:3177910/93‑1 (MQ=255)

                                                            tGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCga                             <  1:3991255/93‑1 (MQ=255)

                                                                 cAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAtt                        <  2:4477850/93‑1 (MQ=255)

                                                                 cAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAtt                        >  1:2705422/1‑93 (MQ=255)

                                                                 cAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAtt                        <  1:3411330/93‑1 (MQ=255)

                                                                 cAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAtt                        <  1:1047522/93‑1 (MQ=255)

                                                                 cAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAtt                        >  1:4272323/1‑93 (MQ=255)

                                                                   gCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTgt                      >  1:1794924/1‑92 (MQ=255)

                                                                    ccATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGt                     <  1:1037513/93‑1 (MQ=255)

                                                                     cATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGtt                    <  2:2166566/93‑1 (MQ=255)

                                                                     cATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGtt                    <  2:3132273/93‑1 (MQ=255)

                                                                      aTTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGttt                   >  1:2973440/1‑93 (MQ=255)

                                                                         gTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTg                <  2:4424625/93‑1 (MQ=255)

                                                                          ttATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGc               >  1:3850266/1‑93 (MQ=255)

                                                                             tGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCg            >  2:3237445/1‑93 (MQ=255)

                                                                               gCCGCAGTTGCGGGTGATCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCggg          <  1:2932290/93‑1 (MQ=255)

                                                                               gCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCggg          >  1:256847/1‑93 (MQ=255)

                                                                               gCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCggg          <  2:136793/93‑1 (MQ=255)

                                                                                ccGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGt         >  2:3396826/1‑93 (MQ=255)

                                                                                  gCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGc       >  2:4305380/1‑93 (MQ=255)

                                                                                   cAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCg      >  1:1930369/1‑93 (MQ=255)

                                                                                    aGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCgg     >  2:3642657/1‑93 (MQ=255)

                                                                                     gTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGa    >  1:4461794/1‑93 (MQ=255)

                                                                                     gTTGCGGGTGAGCGAAGGCGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTCGCCGGGTGCGGa    >  2:2607176/1‑93 (MQ=255)

                                                                                      ttGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAg   <  1:1333136/93‑1 (MQ=255)

TGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGT  >  NC_000913/3792947‑3793126

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGG  >  NC_000913/3792944‑3793130

TAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCG                                                                                               <  SRR4302535.942456/94‑1 (MQ=60)

TAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCG                                                                                               >  SRR4302535.3245305/1‑94 (MQ=60)

    GGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGTACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTG                                                                                           <  SRR4302535.3330598/94‑1 (MQ=60)

    GGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTG                                                                                           <  SRR4302535.1429174/94‑1 (MQ=60)

      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         >  SRR4302535.2889442/1‑94 (MQ=60)

      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         >  SRR4302535.3379824/1‑94 (MQ=60)

      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         >  SRR4302535.2181283/1‑94 (MQ=60)

      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         >  SRR4302535.2181282/1‑94 (MQ=60)

      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         >  SRR4302535.1984669/1‑94 (MQ=60)

      TGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAG                                                                                         <  SRR4302535.1934368/94‑1 (MQ=60)

        GAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCG                                                                                       <  SRR4302535.2918911/94‑1 (MQ=60)

        GAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCG                                                                                       <  SRR4302535.1048083/94‑1 (MQ=60)

         AACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGA                                                                                      <  SRR4302535.4525185/94‑1 (MQ=60)

          ACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAA                                                                                     >  SRR4302535.3892238/1‑94 (MQ=60)

           CGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAG                                                                                    <  SRR4302535.1173965/94‑1 (MQ=60)

           CGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAG                                                                                    <  SRR4302535.2797363/94‑1 (MQ=60)

           CGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAG                                                                                    <  SRR4302535.3184846/94‑1 (MQ=60)

           CGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAG                                                                                    <  SRR4302535.3744851/94‑1 (MQ=60)

           CGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAG                                                                                    <  SRR4302535.511377/94‑1 (MQ=60)

            GCTATATAGCGCTGGCCTATGACAAAGTCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGG                                                                                   <  SRR4302535.129945/94‑1 (MQ=60)

            GCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGG                                                                                   <  SRR4302535.1112410/94‑1 (MQ=60)

            GCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGG                                                                                   <  SRR4302535.2297272/94‑1 (MQ=60)

            GCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGG                                                                                   >  SRR4302535.741113/1‑94 (MQ=60)

             CTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGT                                                                                  >  SRR4302535.610239/1‑94 (MQ=60)

              TATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTG                                                                                 <  SRR4302535.849982/94‑1 (MQ=60)

              TATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTG                                                                                 >  SRR4302535.3409986/1‑94 (MQ=60)

              TATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTG                                                                                 >  SRR4302535.1590893/1‑94 (MQ=60)

                TATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAA                                                                               <  SRR4302535.1675367/94‑1 (MQ=60)

                TATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAA                                                                               <  SRR4302535.1107762/94‑1 (MQ=60)

                TATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAA                                                                               >  SRR4302535.3784592/1‑94 (MQ=60)

                TATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAA                                                                               >  SRR4302535.1172496/1‑94 (MQ=60)

                TATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAA                                                                               >  SRR4302535.1934945/1‑94 (MQ=60)

                 ATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAA                                                                              <  SRR4302535.3300706/94‑1 (MQ=60)

                  TAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAA                                                                             <  SRR4302535.1723422/94‑1 (MQ=60)

                   AGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAA                                                                            <  SRR4302535.598005/94‑1 (MQ=60)

                   AGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAA                                                                            >  SRR4302535.2446236/1‑94 (MQ=60)

                   AGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAA                                                                            <  SRR4302535.19165/94‑1 (MQ=60)

                   AGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAA                                                                            >  SRR4302535.3259616/1‑94 (MQ=60)

                    GCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAAT                                                                           <  SRR4302535.4539359/94‑1 (MQ=60)

                     CGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATC                                                                          <  SRR4302535.1713226/94‑1 (MQ=60)

                     CGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATC                                                                          <  SRR4302535.151233/94‑1 (MQ=60)

                     CGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATC                                                                          >  SRR4302535.2724469/1‑94 (MQ=60)

                      GCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCA                                                                         <  SRR4302535.1072171/94‑1 (MQ=60)

                       CTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCAT                                                                        <  SRR4302535.2016537/94‑1 (MQ=60)

                       CTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGAGAGCGAAGGTGAAAAATAAT                                                                        >  SRR4302535.942350/1‑94 (MQ=60)

                        TGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATA                                                                       <  SRR4302535.1954885/94‑1 (MQ=60)

                         GGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATAT                                                                      <  SRR4302535.3535513/94‑1 (MQ=60)

                         GGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATAT                                                                      >  SRR4302535.2288289/1‑94 (MQ=60)

                          GCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATA                                                                     <  SRR4302535.1900297/94‑1 (MQ=60)

                          GCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATA                                                                     <  SRR4302535.746337/94‑1 (MQ=60)

                            CTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACC                                                                   >  SRR4302535.1173579/1‑94 (MQ=60)

                            CTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACC                                                                   <  SRR4302535.2509408/94‑1 (MQ=60)

                             TATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCT                                                                  <  SRR4302535.3666874/94‑1 (MQ=60)

                              ATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTG                                                                 >  SRR4302535.3872422/1‑94 (MQ=60)

                              ATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTG                                                                 >  SRR4302535.4516156/1‑94 (MQ=60)

                              ATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTG                                                                 >  SRR4302535.4571755/1‑94 (MQ=60)

                               TGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGT                                                                >  SRR4302535.3609875/1‑94 (MQ=60)

                                GACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTA                                                               >  SRR4302535.2744958/1‑94 (MQ=60)

                                GACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTA                                                               >  SRR4302535.3960742/1‑94 (MQ=60)

                                  CAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAAT                                                             <  SRR4302535.2674614/94‑1 (MQ=60)

                                   AAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATC                                                            >  SRR4302535.501524/1‑94 (MQ=60)

                                     AGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAA                                                          >  SRR4302535.303713/1‑94 (MQ=60)

                                     AGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGAGACAAATCATATACCTGTAATCAA                                                          >  SRR4302535.2730741/1‑94 (MQ=60)

                                      GGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAAT                                                         <  SRR4302535.1376065/94‑1 (MQ=60)

                                       GCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATT                                                        >  SRR4302535.4323818/1‑94 (MQ=60)

                                       GCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATT                                                        <  SRR4302535.4389151/94‑1 (MQ=60)

                                       GCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATT                                                        >  SRR4302535.1270988/1‑94 (MQ=60)

                                        CATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTT                                                       >  SRR4302535.3744660/1‑94 (MQ=60)

                                        CATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTT                                                       >  SRR4302535.4181313/1‑94 (MQ=60)

                                        CATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTT                                                       <  SRR4302535.4236806/94‑1 (MQ=60)

                                         ATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTT                                                      >  SRR4302535.9379/1‑94 (MQ=60)

                                          TTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTC                                                     <  SRR4302535.1400847/94‑1 (MQ=60)

                                          TTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCCGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTC                                                     >  SRR4302535.1202803/1‑94 (MQ=60)

                                            ATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGC                                                   >  SRR4302535.4440605/1‑94 (MQ=60)

                                             TGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCT                                                  <  SRR4302535.4421821/94‑1 (MQ=60)

                                             TGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCT                                                  <  SRR4302535.411444/94‑1 (MQ=60)

                                             TGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCT                                                  <  SRR4302535.1439542/94‑1 (MQ=60)

                                             TGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCT                                                  <  SRR4302535.2799511/94‑1 (MQ=60)

                                             TGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCT                                                  <  SRR4302535.3204117/94‑1 (MQ=60)

                                              GCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTT                                                 >  SRR4302535.1740139/1‑94 (MQ=60)

                                               CGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTT                                                >  SRR4302535.2903081/1‑94 (MQ=60)

                                               CGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTT                                                <  SRR4302535.1866426/94‑1 (MQ=60)

                                               CGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTT                                                >  SRR4302535.2078030/1‑94 (MQ=60)

                                                GCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTC                                               <  SRR4302535.3010858/94‑1 (MQ=60)

                                                GCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTC                                               >  SRR4302535.1854035/1‑94 (MQ=60)

                                                GCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTC                                               >  SRR4302535.355232/1‑94 (MQ=60)

                                                 CACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCA                                              <  SRR4302535.502063/94‑1 (MQ=60)

                                                 CACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCA                                              >  SRR4302535.3422994/1‑94 (MQ=60)

                                                  ACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCAT                                             <  SRR4302535.1287180/94‑1 (MQ=60)

                                                   CAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATC                                            <  SRR4302535.1620138/94‑1 (MQ=60)

                                                     GCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAG                                          <  SRR4302535.3290232/94‑1 (MQ=60)

                                                      CACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGGGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGA                                         >  SRR4302535.1904268/1‑94 (MQ=60)

                                                       ACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGCAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAA                                        >  SRR4302535.3876139/1‑94 (MQ=60)

                                                        CAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAC                                       <  SRR4302535.2415878/94‑1 (MQ=60)

                                                         AAGATCTGCCGCAGCTATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACG                                      <  SRR4302535.1171089/94‑1 (MQ=60)

                                                         AAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACG                                      >  SRR4302535.4423834/1‑94 (MQ=60)

                                                         AAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACG                                      >  SRR4302535.2671430/1‑94 (MQ=60)

                                                          AGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATGATATACCTGTAATCAATTTTCGCTTTCATCAGAATGT                                     >  SRR4302535.1791900/1‑94 (MQ=60)

                                                          AGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGT                                     <  SRR4302535.4193125/94‑1 (MQ=60)

                                                           GATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTC                                    <  SRR4302535.464961/94‑1 (MQ=60)

                                                           GATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTC                                    <  SRR4302535.4244530/94‑1 (MQ=60)

                                                            ATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCC                                   >  SRR4302535.3665869/1‑94 (MQ=60)

                                                            ATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCC                                   >  SRR4302535.495096/1‑94 (MQ=60)

                                                            ATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCC                                   <  SRR4302535.2235468/94‑1 (MQ=60)

                                                              CTGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGA                                 >  SRR4302535.1338333/1‑94 (MQ=60)

                                                               TGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGAT                                <  SRR4302535.4018554/94‑1 (MQ=60)

                                                               TGCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGAT                                <  SRR4302535.2514515/94‑1 (MQ=60)

                                                                GCCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATG                               <  SRR4302535.2628017/94‑1 (MQ=60)

                                                                 CCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGA                              >  SRR4302535.296587/1‑94 (MQ=60)

                                                                 CCGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGA                              <  SRR4302535.2542979/94‑1 (MQ=60)

                                                                  cgtcGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAT                             <  SRR4302535.399786/90‑1 (MQ=60)

                                                                  CGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAT                             >  SRR4302535.2063714/1‑94 (MQ=60)

                                                                  CGCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGAT                             >  SRR4302535.2657942/1‑94 (MQ=60)

                                                                   GCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATT                            >  SRR4302535.2724754/1‑94 (MQ=60)

                                                                   GCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATT                            <  SRR4302535.1816075/94‑1 (MQ=60)

                                                                   GCAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATT                            >  SRR4302535.4301569/1‑94 (MQ=60)

                                                                    CAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTG                           <  SRR4302535.1054950/94‑1 (MQ=60)

                                                                    CAGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTG                           <  SRR4302535.3435062/94‑1 (MQ=60)

                                                                     AGCCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGT                          >  SRR4302535.1807900/1‑94 (MQ=60)

                                                                       CCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTT                        <  SRR4302535.1044881/94‑1 (MQ=60)

                                                                       CCATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTT                        <  SRR4302535.2070455/94‑1 (MQ=60)

                                                                        CATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTT                       >  SRR4302535.2994312/1‑94 (MQ=60)

                                                                        CATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTT                       >  SRR4302535.2523471/1‑94 (MQ=60)

                                                                        CATTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTT                       >  SRR4302535.50303/1‑94 (MQ=60)

                                                                          TTGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTT                     <  SRR4302535.2963554/94‑1 (MQ=60)

                                                                           TGTTATGGCGGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTG                    <  SRR4302535.3300806/94‑1 (MQ=60)

                                                                           TGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTG                    >  SRR4302535.3907706/1‑94 (MQ=60)

                                                                           TGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTG                    <  SRR4302535.1486003/94‑1 (MQ=60)

                                                                           TGTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTG                    >  SRR4302535.2728549/1‑94 (MQ=60)

                                                                            GTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGC                   >  SRR4302535.3876761/1‑94 (MQ=60)

                                                                            GTTATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGC                   <  SRR4302535.2395967/94‑1 (MQ=60)

                                                                               ATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCG                >  SRR4302535.3830301/1‑94 (MQ=60)

                                                                               ATGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCG                >  SRR4302535.1058034/1‑94 (MQ=60)

                                                                                TGGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGG               >  SRR4302535.2152418/1‑94 (MQ=60)

                                                                                 GGCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGG              >  SRR4302535.258617/1‑94 (MQ=60)

                                                                                  GCCGCAGTTGCGGGTGATCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGT             <  SRR4302535.2952936/94‑1 (MQ=60)

                                                                                  GCCGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGT             <  SRR4302535.3961386/94‑1 (MQ=60)

                                                                                    CGCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGC           >  SRR4302535.3285691/1‑94 (MQ=60)

                                                                                     GCAGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCG          >  SRR4302535.1944281/1‑94 (MQ=60)

                                                                                       AGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGG        >  SRR4302535.3743690/1‑94 (MQ=60)

                                                                                       AGTTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGA        >  SRR4302535.4492395/1‑94 (MQ=60)

                                                                                         TTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGT      <  SRR4302535.1342644/94‑1 (MQ=60)

                                                                                         TTGCGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGT      >  SRR4302535.1240255/1‑94 (MQ=60)

                                                                                            CGGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTG   <  SRR4302535.4646219/94‑1 (MQ=60)

                                                                                             GGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGATTTTGACCGGGTGCGGAGTTTGG  >  SRR4302535.772412/1‑94 (MQ=60)

TAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGG  >  NC_000913/3792944‑3793130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`